Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42736.t1 | ACJ84005.1 | 92 | 75 | 6 | 0 | 22 | 96 | 117 | 191 | 2.80E-33 | 151 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42736.t1 | P49332 | 60.0 | 90 | 30 | 1 | 7 | 96 | 110 | 193 | 1.5e-22 | 106.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42736.t1 | B7FGX6 | 92.0 | 75 | 6 | 0 | 22 | 96 | 117 | 191 | 2.1e-33 | 151.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051265 | MS.gene42736 | 0.807392 | 5.17E-50 | -1.69E-46 |
| MS.gene053998 | MS.gene42736 | 0.809767 | 1.61E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene42736 | 0.805655 | 1.20E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene42736 | 0.836711 | 7.97E-57 | -1.69E-46 |
| MS.gene055358 | MS.gene42736 | 0.817914 | 2.58E-52 | -1.69E-46 |
| MS.gene059128 | MS.gene42736 | 0.812372 | 4.38E-51 | -1.69E-46 |
| MS.gene059584 | MS.gene42736 | 0.806874 | 6.65E-50 | -1.69E-46 |
| MS.gene060167 | MS.gene42736 | 0.805946 | 1.04E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene42736 | 0.803465 | 3.44E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42736.t1 | MTR_8g056940 | 92.000 | 75 | 6 | 0 | 22 | 96 | 117 | 191 | 1.57e-45 | 145 |
| MS.gene42736.t1 | MTR_3g467430 | 61.905 | 84 | 32 | 0 | 11 | 94 | 111 | 194 | 1.32e-29 | 105 |
| MS.gene42736.t1 | MTR_3g467420 | 63.014 | 73 | 27 | 0 | 22 | 94 | 122 | 194 | 3.17e-26 | 96.7 |
| MS.gene42736.t1 | MTR_2g070150 | 61.333 | 75 | 29 | 0 | 22 | 96 | 117 | 191 | 6.80e-26 | 95.5 |
| MS.gene42736.t1 | MTR_2g070140 | 60.000 | 75 | 30 | 0 | 22 | 96 | 101 | 175 | 1.05e-25 | 95.1 |
| MS.gene42736.t1 | MTR_2g070200 | 60.000 | 75 | 30 | 0 | 22 | 96 | 118 | 192 | 3.30e-24 | 91.7 |
| MS.gene42736.t1 | MTR_2g070070 | 64.516 | 62 | 22 | 0 | 33 | 94 | 128 | 189 | 5.18e-24 | 90.9 |
| MS.gene42736.t1 | MTR_2g070060 | 56.000 | 75 | 33 | 0 | 22 | 96 | 117 | 191 | 8.13e-24 | 90.5 |
| MS.gene42736.t1 | MTR_2g070180 | 57.333 | 75 | 32 | 0 | 22 | 96 | 117 | 191 | 1.28e-23 | 90.1 |
| MS.gene42736.t1 | MTR_2g070210 | 57.534 | 73 | 31 | 0 | 24 | 96 | 121 | 193 | 6.89e-23 | 88.2 |
| MS.gene42736.t1 | MTR_2g070110 | 59.375 | 64 | 26 | 0 | 33 | 96 | 127 | 190 | 2.27e-22 | 86.7 |
| MS.gene42736.t1 | MTR_4g124130 | 57.534 | 73 | 31 | 0 | 22 | 94 | 118 | 190 | 1.13e-21 | 85.1 |
| MS.gene42736.t1 | MTR_4g043640 | 62.903 | 62 | 17 | 2 | 36 | 95 | 29 | 86 | 2.75e-21 | 80.5 |
| MS.gene42736.t1 | MTR_5g076900 | 46.512 | 86 | 46 | 0 | 11 | 96 | 108 | 193 | 5.59e-21 | 83.2 |
| MS.gene42736.t1 | MTR_2g070130 | 55.882 | 68 | 30 | 0 | 27 | 94 | 122 | 189 | 2.08e-20 | 81.6 |
| MS.gene42736.t1 | MTR_2g070120 | 46.667 | 90 | 43 | 1 | 12 | 96 | 102 | 191 | 3.51e-20 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42736.t1 | AT1G78370 | 60.000 | 75 | 30 | 0 | 20 | 94 | 115 | 189 | 1.12e-25 | 95.1 |
| MS.gene42736.t1 | AT1G78360 | 56.790 | 81 | 35 | 0 | 16 | 96 | 112 | 192 | 2.42e-25 | 94.4 |
| MS.gene42736.t1 | AT1G78340 | 62.667 | 75 | 28 | 0 | 22 | 96 | 117 | 191 | 6.71e-25 | 93.2 |
| MS.gene42736.t1 | AT1G17180 | 54.762 | 84 | 38 | 0 | 11 | 94 | 106 | 189 | 9.06e-25 | 93.2 |
| MS.gene42736.t1 | AT1G17170 | 60.494 | 81 | 31 | 1 | 14 | 94 | 109 | 188 | 2.26e-24 | 92.0 |
| MS.gene42736.t1 | AT1G53680 | 58.904 | 73 | 30 | 0 | 22 | 94 | 122 | 194 | 3.60e-24 | 91.7 |
| MS.gene42736.t1 | AT1G78380 | 51.163 | 86 | 42 | 0 | 11 | 96 | 106 | 191 | 3.94e-24 | 91.3 |
| MS.gene42736.t1 | AT1G78320 | 55.882 | 68 | 30 | 0 | 27 | 94 | 95 | 162 | 4.35e-19 | 77.8 |
| MS.gene42736.t1 | AT1G78320 | 55.882 | 68 | 30 | 0 | 27 | 94 | 122 | 189 | 1.08e-18 | 77.4 |
| MS.gene42736.t1 | AT1G17190 | 47.887 | 71 | 37 | 0 | 24 | 94 | 120 | 190 | 2.73e-17 | 73.6 |
| MS.gene42736.t1 | AT3G43800 | 47.887 | 71 | 36 | 1 | 25 | 94 | 124 | 194 | 1.66e-14 | 66.2 |
Find 17 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAAGCTCAAGCCAGATTT+TGG | 0.160142 | 8.2:-54762912 | MS.gene42736:CDS |
| CTTGGAGACCAACCTTATTT+TGG | 0.247910 | 8.2:-54762469 | MS.gene42736:CDS |
| GGAGTTGATTGAAGGCTTAA+AGG | 0.261757 | 8.2:-54762506 | MS.gene42736:CDS |
| CTTTGATCCCTTCTCATAGT+TGG | 0.321127 | 8.2:-54762417 | MS.gene42736:CDS |
| GTGATGTTGGAGGAAGGATA+TGG | 0.346197 | 8.2:-54762563 | MS.gene42736:intron |
| GACAAAATCAGCCCAAAATC+TGG | 0.350630 | 8.2:+54762900 | None:intergenic |
| AATGTGTCACCACCAAAATA+AGG | 0.439985 | 8.2:+54762457 | None:intergenic |
| TGAGATTGAATTGGCAAAGA+AGG | 0.443100 | 8.2:-54762527 | MS.gene42736:CDS |
| GAAGAGAGATGAGATTGAAT+TGG | 0.453197 | 8.2:-54762536 | MS.gene42736:CDS |
| GCAAAGAAGGAGTTGATTGA+AGG | 0.480756 | 8.2:-54762514 | MS.gene42736:CDS |
| TGATGTTGGAGGAAGGATAT+GGG | 0.485671 | 8.2:-54762562 | MS.gene42736:intron |
| AAGGAATTAGAGAATGTGCT+TGG | 0.497037 | 8.2:-54762487 | MS.gene42736:CDS |
| TATAAAACCAACTATGAGAA+GGG | 0.541792 | 8.2:+54762410 | None:intergenic |
| TTTATGCAACTTCAAAGTTG+AGG | 0.544531 | 8.2:-54762377 | MS.gene42736:CDS |
| GTTGGAGGAAGGATATGGGC+TGG | 0.551186 | 8.2:-54762558 | MS.gene42736:intron |
| GTATAAAACCAACTATGAGA+AGG | 0.581803 | 8.2:+54762409 | None:intergenic |
| TGTCACCACCAAAATAAGGT+TGG | 0.621870 | 8.2:+54762461 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGATATTGTTATTTATTTAC+TGG | + | chr8.2:54762444-54762463 | None:intergenic | 15.0% |
| !! | AATGTGTTAAAAAAAAATGC+AGG | - | chr8.2:54762656-54762675 | MS.gene42736:intron | 20.0% |
| !!! | ATTGTTATTTATTTACTGGT+AGG | + | chr8.2:54762440-54762459 | None:intergenic | 20.0% |
| ! | AAATATGTTAAGCCATTGAA+TGG | + | chr8.2:54762414-54762433 | None:intergenic | 25.0% |
| ! | AATATGTTAAGCCATTGAAT+GGG | + | chr8.2:54762413-54762432 | None:intergenic | 25.0% |
| ! | CAACCAAGTCTTTAATAATA+TGG | + | chr8.2:54762595-54762614 | None:intergenic | 25.0% |
| ! | GATCCATATTATTAAAGACT+TGG | - | chr8.2:54762589-54762608 | MS.gene42736:intron | 25.0% |
| ! | TATAAAACCAACTATGAGAA+GGG | + | chr8.2:54762838-54762857 | None:intergenic | 25.0% |
| ! | TGTTAAAAAAAAATGCAGGT+TGG | - | chr8.2:54762660-54762679 | MS.gene42736:intron | 25.0% |
| GTATAAAACCAACTATGAGA+AGG | + | chr8.2:54762839-54762858 | None:intergenic | 30.0% | |
| GTATAGGAAACATTCATCAA+TGG | - | chr8.2:54762540-54762559 | MS.gene42736:CDS | 30.0% | |
| TTTATGCAACTTCAAAGTTG+AGG | - | chr8.2:54762868-54762887 | MS.gene42736:intron | 30.0% | |
| !!! | ACAAGAAGGTAAGTTTTGTT+AGG | - | chr8.2:54762369-54762388 | MS.gene42736:CDS | 30.0% |
| AAGGAATTAGAGAATGTGCT+TGG | - | chr8.2:54762758-54762777 | MS.gene42736:intron | 35.0% | |
| AATGTGTCACCACCAAAATA+AGG | + | chr8.2:54762791-54762810 | None:intergenic | 35.0% | |
| AGGAAACATTCATCAATGGA+TGG | - | chr8.2:54762544-54762563 | MS.gene42736:CDS | 35.0% | |
| ATACTCATATCTCACTAGGA+AGG | + | chr8.2:54762524-54762543 | None:intergenic | 35.0% | |
| GAAGAGAGATGAGATTGAAT+TGG | - | chr8.2:54762709-54762728 | MS.gene42736:intron | 35.0% | |
| TCCTAGTGAGATATGAGTAT+AGG | - | chr8.2:54762524-54762543 | MS.gene42736:CDS | 35.0% | |
| TCCTATACTCATATCTCACT+AGG | + | chr8.2:54762528-54762547 | None:intergenic | 35.0% | |
| TGAGATTGAATTGGCAAAGA+AGG | - | chr8.2:54762718-54762737 | MS.gene42736:intron | 35.0% | |
| !!! | ATTTTGGTGGTGACACATTT+GGG | - | chr8.2:54762792-54762811 | MS.gene42736:intron | 35.0% |
| !!! | TATTTTGGTGGTGACACATT+TGG | - | chr8.2:54762791-54762810 | MS.gene42736:intron | 35.0% |
| AGTGTGAAAAACTGGTTGCT+TGG | - | chr8.2:54762894-54762913 | MS.gene42736:CDS | 40.0% | |
| CATATCTCACTAGGAAGGAA+AGG | + | chr8.2:54762519-54762538 | None:intergenic | 40.0% | |
| CTTTGATCCCTTCTCATAGT+TGG | - | chr8.2:54762828-54762847 | MS.gene42736:intron | 40.0% | |
| GAATGGGACACAAAGAACTT+TGG | + | chr8.2:54762397-54762416 | None:intergenic | 40.0% | |
| GACAAAATCAGCCCAAAATC+TGG | + | chr8.2:54762348-54762367 | None:intergenic | 40.0% | |
| GGTTGCATTAATGTGCAAAG+AGG | - | chr8.2:54762610-54762629 | MS.gene42736:intron | 40.0% | |
| GTGTGAAAAACTGGTTGCTT+GGG | - | chr8.2:54762895-54762914 | MS.gene42736:CDS | 40.0% | |
| GTTCTTTGTGTCCCATTCAA+TGG | - | chr8.2:54762399-54762418 | MS.gene42736:CDS | 40.0% | |
| TGATGTTGGAGGAAGGATAT+GGG | - | chr8.2:54762683-54762702 | MS.gene42736:intron | 40.0% | |
| TGTCACCACCAAAATAAGGT+TGG | + | chr8.2:54762787-54762806 | None:intergenic | 40.0% | |
| ! | AGAAAGCTCAAGCCAGATTT+TGG | - | chr8.2:54762333-54762352 | MS.gene42736:CDS | 40.0% |
| ! | GAAAGCTCAAGCCAGATTTT+GGG | - | chr8.2:54762334-54762353 | MS.gene42736:CDS | 40.0% |
| ! | GCAAAGAAGGAGTTGATTGA+AGG | - | chr8.2:54762731-54762750 | MS.gene42736:intron | 40.0% |
| ! | GGAGTTGATTGAAGGCTTAA+AGG | - | chr8.2:54762739-54762758 | MS.gene42736:intron | 40.0% |
| ! | TGAGGAAGAGTGTGAAAAAC+TGG | - | chr8.2:54762886-54762905 | MS.gene42736:intron | 40.0% |
| !! | AAAATGCAGGTTGGTGATGT+TGG | - | chr8.2:54762669-54762688 | MS.gene42736:intron | 40.0% |
| !! | CTTGGAGACCAACCTTATTT+TGG | - | chr8.2:54762776-54762795 | MS.gene42736:intron | 40.0% |
| !! | GGCTGATTTTGTCAACAAGA+AGG | - | chr8.2:54762355-54762374 | MS.gene42736:CDS | 40.0% |
| GTGATGTTGGAGGAAGGATA+TGG | - | chr8.2:54762682-54762701 | MS.gene42736:intron | 45.0% | |
| !!! | GGAGACCAACCTTATTTTGG+TGG | - | chr8.2:54762779-54762798 | MS.gene42736:intron | 45.0% |
| !! | AGGTTGGTGATGTTGGAGGA+AGG | - | chr8.2:54762676-54762695 | MS.gene42736:intron | 50.0% |
| !! | ATGCAGGTTGGTGATGTTGG+AGG | - | chr8.2:54762672-54762691 | MS.gene42736:intron | 50.0% |
| GTTGGAGGAAGGATATGGGC+TGG | - | chr8.2:54762687-54762706 | MS.gene42736:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 54762330 | 54762937 | 54762330 | ID=MS.gene42736 |
| chr8.2 | mRNA | 54762330 | 54762937 | 54762330 | ID=MS.gene42736.t1;Parent=MS.gene42736 |
| chr8.2 | exon | 54762891 | 54762937 | 54762891 | ID=MS.gene42736.t1.exon1;Parent=MS.gene42736.t1 |
| chr8.2 | CDS | 54762891 | 54762937 | 54762891 | ID=cds.MS.gene42736.t1;Parent=MS.gene42736.t1 |
| chr8.2 | exon | 54762330 | 54762573 | 54762330 | ID=MS.gene42736.t1.exon2;Parent=MS.gene42736.t1 |
| chr8.2 | CDS | 54762330 | 54762573 | 54762330 | ID=cds.MS.gene42736.t1;Parent=MS.gene42736.t1 |
| Gene Sequence |
| Protein sequence |
