Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43448.t1 | ONM08445.1 | 70.8 | 96 | 6 | 1 | 38 | 111 | 614 | 709 | 5.10E-26 | 127.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43448.t1 | Q53RK8 | 53.3 | 120 | 10 | 1 | 38 | 111 | 612 | 731 | 5.3e-24 | 111.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43448.t1 | A0A1D6L1P0 | 70.8 | 96 | 6 | 1 | 38 | 111 | 614 | 709 | 3.7e-26 | 127.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene43448 | 0.814676 | 1.36E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene43448 | 0.806471 | 8.10E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene43448 | 0.804677 | 1.93E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene43448 | 0.809896 | 1.51E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene43448 | 0.816727 | 4.76E-52 | -1.69E-46 |
| MS.gene058783 | MS.gene43448 | 0.838027 | 3.67E-57 | -1.69E-46 |
| MS.gene058979 | MS.gene43448 | 0.802473 | 5.52E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43448.t1 | MTR_4g134170 | 60.000 | 120 | 2 | 1 | 38 | 111 | 583 | 702 | 3.78e-36 | 129 |
| MS.gene43448.t1 | MTR_6g005320 | 59.167 | 120 | 3 | 1 | 38 | 111 | 543 | 662 | 7.98e-35 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43448.t1 | AT2G33730 | 51.667 | 120 | 12 | 1 | 38 | 111 | 610 | 729 | 2.09e-30 | 113 |
Find 30 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATAGATCCTGGCTTGAATT+TGG | 0.231521 | 8.4:+611735 | None:intergenic |
| AGATTAGTCTTGAAGGATTT+AGG | 0.252142 | 8.4:-612022 | MS.gene43448:CDS |
| AAGAAAATCAGCGCTCTTCT+TGG | 0.298072 | 8.4:+612113 | None:intergenic |
| GCTTCATGCCTTGCCAATTC+AGG | 0.302855 | 8.4:+611760 | None:intergenic |
| CAGTCCTGTTCCACCTGAAT+TGG | 0.333615 | 8.4:-611773 | MS.gene43448:CDS |
| CTTGCCAATTCAGGTGGAAC+AGG | 0.427377 | 8.4:+611769 | None:intergenic |
| AACTAGCACATTATATCTCT+TGG | 0.431019 | 8.4:+611998 | None:intergenic |
| CTAGTTGCAACTGATGTTGC+TGG | 0.452182 | 8.4:-611981 | MS.gene43448:CDS |
| CAGAGGGAGATTAGTCTTGA+AGG | 0.474519 | 8.4:-612029 | MS.gene43448:CDS |
| GTTGATACATGAGCCACATC+AGG | 0.495060 | 8.4:+611942 | None:intergenic |
| CTTTGCATGGAGGGAATCAC+AGG | 0.505844 | 8.4:-612054 | MS.gene43448:CDS |
| TATCGCGTGACCACTTTGCA+TGG | 0.515234 | 8.4:-612067 | MS.gene43448:CDS |
| ATTTCGCCTAGGTGGTCTGT+CGG | 0.525141 | 8.4:+611711 | None:intergenic |
| GCGTGACCACTTTGCATGGA+GGG | 0.527382 | 8.4:-612063 | MS.gene43448:CDS |
| GGTCTGTCGGGAATAGATCC+TGG | 0.531479 | 8.4:+611724 | None:intergenic |
| TTTCGCCTAGGTGGTCTGTC+GGG | 0.533463 | 8.4:+611712 | None:intergenic |
| TGCTAGAATTTGGACAAGGA+AGG | 0.542178 | 8.4:-612091 | MS.gene43448:CDS |
| GCAACTGATGTTGCTGGACG+TGG | 0.547602 | 8.4:-611975 | MS.gene43448:CDS |
| TCATGCCTTGCCAATTCAGG+TGG | 0.578546 | 8.4:+611763 | None:intergenic |
| GGAGGGAATCACAGGATCAG+AGG | 0.582546 | 8.4:-612046 | MS.gene43448:CDS |
| GAAGCTTCCAAATTCAAGCC+AGG | 0.584125 | 8.4:-611742 | MS.gene43448:CDS |
| GAGGGAATCACAGGATCAGA+GGG | 0.596478 | 8.4:-612045 | MS.gene43448:CDS |
| CTGTTCCACCTGAATTGGCA+AGG | 0.607946 | 8.4:-611768 | MS.gene43448:CDS |
| CGCGTGACCACTTTGCATGG+AGG | 0.615726 | 8.4:-612064 | MS.gene43448:CDS |
| GTGATTCCCTCCATGCAAAG+TGG | 0.625177 | 8.4:+612057 | None:intergenic |
| AAAACAGTGTCATTTCGCCT+AGG | 0.628001 | 8.4:+611700 | None:intergenic |
| ACAGTGTCATTTCGCCTAGG+TGG | 0.641491 | 8.4:+611703 | None:intergenic |
| TGGTATTGACATTCCTGATG+TGG | 0.647427 | 8.4:-611955 | MS.gene43448:CDS |
| TTCTTGCTAGAATTTGGACA+AGG | 0.654083 | 8.4:-612095 | MS.gene43448:CDS |
| CTATTCCCGACAGACCACCT+AGG | 0.700511 | 8.4:-611717 | MS.gene43448:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACTAGCACATTATATCTCT+TGG | + | chr8.4:611862-611881 | None:intergenic | 30.0% | |
| ! | CTGATTTTCTTGCTAGAATT+TGG | - | chr8.4:611756-611775 | MS.gene43448:CDS | 30.0% |
| !! | AGATTAGTCTTGAAGGATTT+AGG | - | chr8.4:611835-611854 | MS.gene43448:intron | 30.0% |
| AAATGTACACCCATCGTATT+GGG | - | chr8.4:611960-611979 | MS.gene43448:CDS | 35.0% | |
| GTATCAACTATGATATGCCT+GGG | - | chr8.4:611930-611949 | MS.gene43448:intron | 35.0% | |
| GTGTACATTTCAATATTCCC+AGG | + | chr8.4:611950-611969 | None:intergenic | 35.0% | |
| TGTATCAACTATGATATGCC+TGG | - | chr8.4:611929-611948 | MS.gene43448:intron | 35.0% | |
| TTCTTGCTAGAATTTGGACA+AGG | - | chr8.4:611762-611781 | MS.gene43448:CDS | 35.0% | |
| ! | AATAGATCCTGGCTTGAATT+TGG | + | chr8.4:612125-612144 | None:intergenic | 35.0% |
| AAGAAAATCAGCGCTCTTCT+TGG | + | chr8.4:611747-611766 | None:intergenic | 40.0% | |
| GAAATGTACACCCATCGTAT+TGG | - | chr8.4:611959-611978 | MS.gene43448:CDS | 40.0% | |
| TACAACATTCCTGACTCTTC+AGG | - | chr8.4:612015-612034 | MS.gene43448:CDS | 40.0% | |
| TGCTAGAATTTGGACAAGGA+AGG | - | chr8.4:611766-611785 | MS.gene43448:CDS | 40.0% | |
| !! | TGGTATTGACATTCCTGATG+TGG | - | chr8.4:611902-611921 | MS.gene43448:intron | 40.0% |
| CTAGTTGCAACTGATGTTGC+TGG | - | chr8.4:611876-611895 | MS.gene43448:intron | 45.0% | |
| GAAGCTTCCAAATTCAAGCC+AGG | - | chr8.4:612115-612134 | MS.gene43448:CDS | 45.0% | |
| GTTGATACATGAGCCACATC+AGG | + | chr8.4:611918-611937 | None:intergenic | 45.0% | |
| !! | CAGAGGGAGATTAGTCTTGA+AGG | - | chr8.4:611828-611847 | MS.gene43448:intron | 45.0% |
| ACCAGTACGCCCAATACGAT+GGG | + | chr8.4:611972-611991 | None:intergenic | 50.0% | |
| ATTTCGCCTAGGTGGTCTGT+CGG | + | chr8.4:612149-612168 | None:intergenic | 50.0% | |
| CAGTCCTGTTCCACCTGAAT+TGG | - | chr8.4:612084-612103 | MS.gene43448:CDS | 50.0% | |
| CATCAGTGTCCTGAAGAGTC+AGG | + | chr8.4:612027-612046 | None:intergenic | 50.0% | |
| CTGTTCCACCTGAATTGGCA+AGG | - | chr8.4:612089-612108 | MS.gene43448:CDS | 50.0% | |
| CTTGCCAATTCAGGTGGAAC+AGG | + | chr8.4:612091-612110 | None:intergenic | 50.0% | |
| CTTTGCATGGAGGGAATCAC+AGG | - | chr8.4:611803-611822 | MS.gene43448:intron | 50.0% | |
| GAGGGAATCACAGGATCAGA+GGG | - | chr8.4:611812-611831 | MS.gene43448:intron | 50.0% | |
| GCTTCATGCCTTGCCAATTC+AGG | + | chr8.4:612100-612119 | None:intergenic | 50.0% | |
| GTGATTCCCTCCATGCAAAG+TGG | + | chr8.4:611803-611822 | None:intergenic | 50.0% | |
| TACTGGTCGTGCAGGAAAGA+CGG | - | chr8.4:611985-612004 | MS.gene43448:CDS | 50.0% | |
| TATCGCGTGACCACTTTGCA+TGG | - | chr8.4:611790-611809 | MS.gene43448:CDS | 50.0% | |
| TCATGCCTTGCCAATTCAGG+TGG | + | chr8.4:612097-612116 | None:intergenic | 50.0% | |
| ACCCATCGTATTGGGCGTAC+TGG | - | chr8.4:611968-611987 | MS.gene43448:CDS | 55.0% | |
| ACTGGTCGTGCAGGAAAGAC+GGG | - | chr8.4:611986-612005 | MS.gene43448:CDS | 55.0% | |
| CTATTCCCGACAGACCACCT+AGG | - | chr8.4:612140-612159 | MS.gene43448:CDS | 55.0% | |
| GACCAGTACGCCCAATACGA+TGG | + | chr8.4:611973-611992 | None:intergenic | 55.0% | |
| GCAACTGATGTTGCTGGACG+TGG | - | chr8.4:611882-611901 | MS.gene43448:intron | 55.0% | |
| GCGTGACCACTTTGCATGGA+GGG | - | chr8.4:611794-611813 | MS.gene43448:intron | 55.0% | |
| GGAGGGAATCACAGGATCAG+AGG | - | chr8.4:611811-611830 | MS.gene43448:intron | 55.0% | |
| GGTCTGTCGGGAATAGATCC+TGG | + | chr8.4:612136-612155 | None:intergenic | 55.0% | |
| TTTCGCCTAGGTGGTCTGTC+GGG | + | chr8.4:612148-612167 | None:intergenic | 55.0% | |
| CGCGTGACCACTTTGCATGG+AGG | - | chr8.4:611793-611812 | MS.gene43448:CDS | 60.0% | |
| TCGTGCAGGAAAGACGGGTG+TGG | - | chr8.4:611991-612010 | MS.gene43448:CDS | 60.0% | |
| ! | ATTGGGCGTACTGGTCGTGC+AGG | - | chr8.4:611977-611996 | MS.gene43448:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 611705 | 612174 | 611705 | ID=MS.gene43448 |
| chr8.4 | mRNA | 611705 | 612174 | 611705 | ID=MS.gene43448.t1;Parent=MS.gene43448 |
| chr8.4 | exon | 611950 | 612174 | 611950 | ID=MS.gene43448.t1.exon1;Parent=MS.gene43448.t1 |
| chr8.4 | CDS | 611950 | 612174 | 611950 | ID=cds.MS.gene43448.t1;Parent=MS.gene43448.t1 |
| chr8.4 | exon | 611705 | 611812 | 611705 | ID=MS.gene43448.t1.exon2;Parent=MS.gene43448.t1 |
| chr8.4 | CDS | 611705 | 611812 | 611705 | ID=cds.MS.gene43448.t1;Parent=MS.gene43448.t1 |
| Gene Sequence |
| Protein sequence |
