AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43756

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43756.t1 MTR_5g069710 96.091 307 12 0 1 307 1 307 0.0 617
MS.gene43756.t1 MTR_3g462790 66.565 329 81 9 1 307 1 322 1.02e-148 420
MS.gene43756.t1 MTR_7g067080 58.974 312 113 8 1 303 7 312 2.89e-117 340
MS.gene43756.t1 MTR_5g081860 62.871 202 68 3 4 203 2 198 2.85e-86 260
MS.gene43756.t1 MTR_1g111830 50.282 177 79 2 27 200 22 192 2.54e-56 182
MS.gene43756.t1 MTR_4g111975 44.538 238 94 5 29 233 8 240 4.68e-56 184
MS.gene43756.t1 MTR_8g063600 44.550 211 107 4 22 230 3 205 7.16e-51 170
MS.gene43756.t1 MTR_8g063870 36.000 250 128 5 23 270 2 221 1.65e-42 147
MS.gene43756.t1 MTR_4g100630 58.182 110 36 2 90 196 57 159 9.60e-32 120
MS.gene43756.t1 MTR_2g100930 73.239 71 19 0 127 197 104 174 1.29e-31 121
MS.gene43756.t1 MTR_6g092540 52.632 114 49 1 126 239 91 199 4.73e-31 118
MS.gene43756.t1 MTR_8g101650 69.333 75 22 1 123 197 96 169 3.04e-30 115
MS.gene43756.t1 MTR_4g015130 80.645 62 12 0 142 203 3 64 5.08e-30 109
MS.gene43756.t1 MTR_5g037080 70.513 78 23 0 126 203 96 173 1.47e-28 112
MS.gene43756.t1 MTR_4g107230 66.667 81 27 0 122 202 74 154 9.06e-27 106
MS.gene43756.t1 MTR_3g104370 48.780 123 49 4 129 240 88 207 3.25e-26 103
MS.gene43756.t1 MTR_5g088010 53.846 91 38 2 124 211 52 141 6.49e-25 100
MS.gene43756.t1 MTR_1g033620 59.551 89 35 1 148 236 21 108 1.21e-23 97.4
MS.gene43756.t1 MTR_0036s0260 64.179 67 24 0 132 198 28 94 2.01e-23 95.9
MS.gene43756.t1 MTR_5g088060 59.259 81 29 2 134 211 274 353 4.90e-23 99.0
MS.gene43756.t1 MTR_5g488160 59.259 81 29 2 134 211 274 353 4.90e-23 99.0
MS.gene43756.t1 MTR_2g090305 64.179 67 24 0 132 198 28 94 9.96e-23 93.6
MS.gene43756.t1 MTR_5g027570 55.814 86 35 2 128 211 88 172 1.45e-22 94.4
MS.gene43756.t1 MTR_5g075760 35.882 170 89 7 23 188 7 160 6.30e-22 92.0
MS.gene43756.t1 MTR_5g075790 35.088 171 88 6 23 188 16 168 2.30e-21 90.9
MS.gene43756.t1 MTR_1g033600 35.338 133 69 2 26 146 9 136 1.57e-20 86.3
MS.gene43756.t1 MTR_5g027550 56.322 87 34 2 128 211 73 158 3.78e-20 87.4
MS.gene43756.t1 MTR_1g022290 49.315 73 37 0 24 96 2 74 8.12e-20 82.8
MS.gene43756.t1 MTR_8g077360 51.389 72 34 1 25 96 1 71 7.25e-19 79.7
MS.gene43756.t1 MTR_8g077380 51.389 72 34 1 25 96 1 71 7.36e-19 79.7
MS.gene43756.t1 MTR_8g077390 51.389 72 34 1 25 96 1 71 7.36e-19 79.7
MS.gene43756.t1 MTR_3g116720 52.308 65 31 0 29 93 16 80 7.53e-19 79.7
MS.gene43756.t1 MTR_1g048660 56.250 64 28 0 133 196 13 76 8.25e-19 80.1
MS.gene43756.t1 MTR_3g111880 54.545 66 30 0 26 91 4 69 1.43e-18 79.3
MS.gene43756.t1 MTR_5g020170 46.875 64 34 0 28 91 18 81 1.63e-18 79.3
MS.gene43756.t1 MTR_6g004250 47.297 74 39 0 24 97 5 78 9.47e-18 76.6
MS.gene43756.t1 MTR_3g013440 45.455 88 36 2 24 110 5 81 5.92e-16 72.4
MS.gene43756.t1 MTR_8g077420 49.231 65 32 1 25 89 1 64 1.07e-15 70.9
MS.gene43756.t1 MTR_5g020540 50.820 61 29 1 29 89 4 63 5.55e-15 68.9
MS.gene43756.t1 MTR_3g070110 37.984 129 67 2 80 201 49 171 1.34e-14 72.0
MS.gene43756.t1 MTR_7g089210 53.333 60 28 0 32 91 15 74 3.14e-14 67.4
MS.gene43756.t1 MTR_1g083180 52.055 73 30 2 134 202 63 134 2.76e-13 67.4
MS.gene43756.t1 MTR_6g043480 46.774 62 32 1 117 178 93 153 9.65e-12 63.9
MS.gene43756.t1 MTR_3g111920 44.444 63 35 0 29 91 3 65 9.73e-12 60.5
MS.gene43756.t1 MTR_8g072990 43.056 72 36 2 129 196 97 167 2.12e-11 63.2
MS.gene43756.t1 MTR_6g043490 53.191 47 22 0 132 178 107 153 2.50e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43756.t1 AT2G38090 65.865 208 58 2 5 201 5 210 8.76e-99 293
MS.gene43756.t1 AT5G58900 68.224 214 53 4 5 204 1 213 3.64e-95 283
MS.gene43756.t1 AT5G01200 61.500 200 62 5 16 201 17 215 3.23e-78 239
MS.gene43756.t1 AT5G05790 61.275 204 68 5 5 201 1 200 8.41e-78 238
MS.gene43756.t1 AT5G05790 61.275 204 68 5 5 201 1 200 8.41e-78 238
MS.gene43756.t1 AT3G11280 67.052 173 48 2 29 201 33 196 8.69e-78 238
MS.gene43756.t1 AT3G11280 67.052 173 48 2 29 201 33 196 8.69e-78 238
MS.gene43756.t1 AT1G49010 51.232 203 72 5 23 200 2 202 7.91e-55 181
MS.gene43756.t1 AT5G08520 50.273 183 86 3 22 200 5 186 5.84e-54 178
MS.gene43756.t1 AT5G04760 41.892 222 116 3 26 244 3 214 1.52e-50 167
MS.gene43756.t1 AT5G23650 41.538 195 88 3 18 197 2 185 6.00e-42 148
MS.gene43756.t1 AT5G47390 60.185 108 35 3 92 197 60 161 1.21e-31 121
MS.gene43756.t1 AT3G10580 41.573 178 80 7 23 197 2 158 1.22e-31 119
MS.gene43756.t1 AT3G10580 41.573 178 80 7 23 197 2 158 1.88e-31 119
MS.gene43756.t1 AT4G09450 39.572 187 93 5 28 214 6 172 1.42e-30 114
MS.gene43756.t1 AT5G61620 48.819 127 63 1 127 253 103 227 1.47e-28 112
MS.gene43756.t1 AT5G56840 50.000 104 51 1 98 200 57 160 3.97e-27 106
MS.gene43756.t1 AT1G70000 70.833 72 21 0 126 197 92 163 1.32e-26 105
MS.gene43756.t1 AT1G70000 70.833 72 21 0 126 197 92 163 1.32e-26 105
MS.gene43756.t1 AT3G16350 72.222 72 20 0 127 198 132 203 1.98e-25 105
MS.gene43756.t1 AT1G74840 70.833 72 21 0 127 198 91 162 6.64e-25 100
MS.gene43756.t1 AT1G74840 70.833 72 21 0 127 198 91 162 8.72e-25 100
MS.gene43756.t1 AT1G19000 52.727 110 48 1 128 237 98 203 2.27e-24 100
MS.gene43756.t1 AT1G19000 52.727 110 48 1 128 237 98 203 2.27e-24 100
MS.gene43756.t1 AT1G75250 55.714 70 31 0 24 93 7 76 2.00e-21 88.2
MS.gene43756.t1 AT1G75250 55.714 70 31 0 24 93 7 76 2.04e-21 87.4
MS.gene43756.t1 AT3G10590 35.912 181 98 6 26 197 4 175 4.92e-21 89.4
MS.gene43756.t1 AT2G21650 52.941 68 32 0 29 96 14 81 9.25e-18 77.4
MS.gene43756.t1 AT4G39250 49.231 65 33 0 29 93 14 78 1.44e-16 74.3
MS.gene43756.t1 AT1G19510 51.613 62 30 0 32 93 15 76 2.13e-15 71.2
MS.gene43756.t1 AT2G18328 52.542 59 28 0 33 91 15 73 4.95e-13 63.9
MS.gene43756.t1 AT4G36570 56.818 44 19 0 29 72 12 55 1.03e-11 59.7

Find 59 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGACCAAAATCTTCAAAA+AGG 0.271482 5.1:+54075221 MS.gene43756:CDS
TATTTCATAAGACAGCTTTC+AGG 0.304151 5.1:+54074927 MS.gene43756:CDS
CACCTTTGCCATACTTCTTT+AGG 0.307378 5.1:-54074833 None:intergenic
TTAGATTGGGTGAACAGTTC+TGG 0.315891 5.1:+54074143 MS.gene43756:CDS
CCTTTCTTCCTTTCCTGCTC+AGG 0.322349 5.1:-54074215 None:intergenic
CTTCACCCTTTACCTTAGAT+TGG 0.332398 5.1:+54074129 MS.gene43756:CDS
CTACTAGGCCTAAAGAAGTA+TGG 0.375050 5.1:+54074825 MS.gene43756:CDS
GCTGGGTTGATTCCAGTTCC+TGG 0.382171 5.1:+54074092 MS.gene43756:CDS
CGGTGAAGTGGATCTATTAC+CGG 0.382561 5.1:-54075036 None:intergenic
ACATCACCGACCGTCTTTCC+TGG 0.388704 5.1:-54074023 None:intergenic
AGAACTGTTCACCCAATCTA+AGG 0.390954 5.1:-54074141 None:intergenic
GATGTTTGTAATATAGAAGC+TGG 0.393501 5.1:+54074074 MS.gene43756:CDS
AACATTATTCACATGCATAA+AGG 0.404368 5.1:+54075303 MS.gene43756:CDS
TGATAAGATGAAGTGGGAAA+TGG 0.405855 5.1:+54073839 None:intergenic
AAGTTTGTTGGTGCTGCTGC+TGG 0.410002 5.1:-54075082 None:intergenic
TGATGTGATGAGACAATATA+AGG 0.419767 5.1:+54074040 MS.gene43756:CDS
AGAAGTATGGCAAAGGTGAT+TGG 0.420618 5.1:+54074838 MS.gene43756:CDS
TTCACCCTTTACCTTAGATT+GGG 0.424588 5.1:+54074130 MS.gene43756:CDS
GCTATCTTCCATACACCAAT+TGG 0.448071 5.1:-54073895 None:intergenic
TGCTCATGAGCTGGATTGAA+AGG 0.456365 5.1:-54075152 None:intergenic
TTGTGTTCTTCTTCAGTCCA+TGG 0.457416 5.1:-54074242 None:intergenic
CATACACACAAAACTCCAAT+TGG 0.463612 5.1:+54073880 MS.gene43756:CDS
TATGATGAATTCAGAGGAAG+TGG 0.470044 5.1:+54074167 MS.gene43756:CDS
GCATGGCTTGCTACTTGAGT+TGG 0.471843 5.1:-54074897 None:intergenic
GGAGTGTTATAACCAGGAAC+TGG 0.478409 5.1:-54074104 None:intergenic
TTCACCCAATCTAAGGTAAA+GGG 0.481797 5.1:-54074134 None:intergenic
ACAAAACTCCAATTGGTGTA+TGG 0.484625 5.1:+54073887 MS.gene43756:CDS
GTTCACCCAATCTAAGGTAA+AGG 0.487408 5.1:-54074135 None:intergenic
TCCTGCAGATTATTTCTACT+AGG 0.501666 5.1:+54074810 MS.gene43756:intron
ATGAAAACTTGCTCATGAGC+TGG 0.506678 5.1:-54075161 None:intergenic
TCTAAGGTAAAGGGTGAAGT+AGG 0.513528 5.1:-54074125 None:intergenic
TTAGAGCTCCTGAGCAGGAA+AGG 0.515723 5.1:+54074207 MS.gene43756:CDS
CCTGAGCAGGAAAGGAAGAA+AGG 0.521387 5.1:+54074215 MS.gene43756:CDS
CATAGAATTCTGCGGTGAAG+TGG 0.521435 5.1:-54075048 None:intergenic
TTTAGTTAGAGCTCCTGAGC+AGG 0.523673 5.1:+54074202 MS.gene43756:CDS
GAAAACTTGATAAGATGAAG+TGG 0.527680 5.1:+54073832 None:intergenic
GCTACTTTATGCCATCGATC+CGG 0.541949 5.1:-54073990 None:intergenic
ATAAAGATACGCCGGATCGA+TGG 0.544701 5.1:+54073979 MS.gene43756:CDS
TTGTTGCCGTCGAAGTTTGT+TGG 0.545215 5.1:-54075094 None:intergenic
TTCATAAGACAGCTTTCAGG+AGG 0.585837 5.1:+54074930 MS.gene43756:CDS
CTCTTCTGGAGTCCATTGTG+TGG 0.588727 5.1:-54073922 None:intergenic
AAAGTAGCTGAAATGATACC+AGG 0.595280 5.1:+54074005 MS.gene43756:CDS
ATGTTTGTAATATAGAAGCT+GGG 0.604659 5.1:+54074075 MS.gene43756:CDS
AAAACTTGATAAGATGAAGT+GGG 0.612600 5.1:+54073833 None:intergenic
GGCCTAAAGAAGTATGGCAA+AGG 0.616426 5.1:+54074831 MS.gene43756:CDS
AAAGGAAGAAAGGAGTGCCA+TGG 0.621552 5.1:+54074225 MS.gene43756:CDS
TGAAATGATACCAGGAAAGA+CGG 0.622184 5.1:+54074013 MS.gene43756:CDS
TCTGGTTATGATGAATTCAG+AGG 0.623212 5.1:+54074161 MS.gene43756:CDS
ATGATACCAGGAAAGACGGT+CGG 0.635685 5.1:+54074017 MS.gene43756:CDS
GAAGTAGGAGTGTTATAACC+AGG 0.644855 5.1:-54074110 None:intergenic
TTTCAGGAGGAAAAGACAAG+AGG 0.650765 5.1:+54074943 MS.gene43756:CDS
GCAGCACCAACAAACTTCGA+CGG 0.651047 5.1:+54075088 MS.gene43756:CDS
TTTGTGTGTATGATGCAGGA+GGG 0.665325 5.1:-54073869 None:intergenic
AGTTCATGATAAAGATACGC+CGG 0.676724 5.1:+54073971 MS.gene43756:CDS
AAGATAGCATGACCACACAA+TGG 0.678472 5.1:+54073910 MS.gene43756:CDS
GTCTTCGTATCCACATAACA+TGG 0.678504 5.1:+54075256 MS.gene43756:CDS
GAAAGTAACATAGAATTCTG+CGG 0.686589 5.1:-54075056 None:intergenic
ACTTGTTCTTCAGAAGACAC+CGG 0.691433 5.1:+54075017 MS.gene43756:CDS
TCATAGGAGTTAAAACCATG+AGG 0.695559 5.1:-54075188 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATTCTAACTTAAGA+TGG - chr5.1:54074791-54074810 None:intergenic 15.0%
!! AATAACAAAACAAATACAAA+AGG - chr5.1:54074280-54074299 None:intergenic 15.0%
!!! TTAGTATTTTTTCTTTACTA+GGG + chr5.1:54074349-54074368 MS.gene43756:intron 15.0%
!! ATATCATCTTAAGTTAGAAT+AGG + chr5.1:54074713-54074732 MS.gene43756:intron 20.0%
!! GTTAATCTTTCAGAAAAAAT+TGG + chr5.1:54074993-54075012 MS.gene43756:CDS 20.0%
!!! ATGATATAAGTTAGTTTTGT+AGG + chr5.1:54074738-54074757 MS.gene43756:intron 20.0%
!!! GTTAGTATTTTTTCTTTACT+AGG + chr5.1:54074348-54074367 MS.gene43756:intron 20.0%
!!! GTTGAATATTTTCATTTTTC+AGG + chr5.1:54074565-54074584 MS.gene43756:intron 20.0%
!!! TGATATAAGTTAGTTTTGTA+GGG + chr5.1:54074739-54074758 MS.gene43756:intron 20.0%
!!! TTAAGAAAGTATAGGAATAA+TGG + chr5.1:54074473-54074492 MS.gene43756:intron 20.0%
! AAGACCAAAATCTTCAAAAA+GGG + chr5.1:54075222-54075241 MS.gene43756:CDS 25.0%
! ATGTTTGTAATATAGAAGCT+GGG + chr5.1:54074075-54074094 MS.gene43756:CDS 25.0%
! ATTTGAAAAACCATGTTATG+TGG - chr5.1:54075269-54075288 None:intergenic 25.0%
! CTTTACAAAATCATCTTGTA+AGG - chr5.1:54074670-54074689 None:intergenic 25.0%
!! TCAAATATTTTGTTCTCTTC+TGG - chr5.1:54073939-54073958 None:intergenic 25.0%
!! TTATGAAATACTTTTGAGCA+TGG - chr5.1:54074917-54074936 None:intergenic 25.0%
!!! ATAATGGATGGAATTTACAA+AGG + chr5.1:54074489-54074508 MS.gene43756:intron 25.0%
!!! GTTTTTTAGCTTTCACTTTA+AGG + chr5.1:54074598-54074617 MS.gene43756:intron 25.0%
!!! TCATTTTTCAGGTTGTAATA+AGG + chr5.1:54074576-54074595 MS.gene43756:intron 25.0%
CAAAATCATCTTGTAAGGTA+AGG - chr5.1:54074665-54074684 None:intergenic 30.0%
CAAGACCAAAATCTTCAAAA+AGG + chr5.1:54075221-54075240 MS.gene43756:CDS 30.0%
CTAACTTAAGATGATATTCG+AGG - chr5.1:54074710-54074729 None:intergenic 30.0%
GAAAGTAACATAGAATTCTG+CGG - chr5.1:54075059-54075078 None:intergenic 30.0%
GATGTTTGTAATATAGAAGC+TGG + chr5.1:54074074-54074093 MS.gene43756:CDS 30.0%
TACAAAGGTAATTTAGACCT+AGG + chr5.1:54074504-54074523 MS.gene43756:intron 30.0%
TATTTCATAAGACAGCTTTC+AGG + chr5.1:54074927-54074946 MS.gene43756:CDS 30.0%
TGATGTGATGAGACAATATA+AGG + chr5.1:54074040-54074059 MS.gene43756:CDS 30.0%
! AAGATGATATTCGAGGATTT+TGG - chr5.1:54074703-54074722 None:intergenic 30.0%
! GAAAGTATAGGAATAATGGA+TGG + chr5.1:54074477-54074496 MS.gene43756:intron 30.0%
! TTGTGAGCTTAAGAAAGTAT+AGG + chr5.1:54074465-54074484 MS.gene43756:intron 30.0%
AAAGGTAATTTAGACCTAGG+AGG + chr5.1:54074507-54074526 MS.gene43756:intron 35.0%
AAAGTAGCTGAAATGATACC+AGG + chr5.1:54074005-54074024 MS.gene43756:CDS 35.0%
ACAAAACTCCAATTGGTGTA+TGG + chr5.1:54073887-54073906 MS.gene43756:CDS 35.0%
AGTTCATGATAAAGATACGC+CGG + chr5.1:54073971-54073990 MS.gene43756:CDS 35.0%
CATACACACAAAACTCCAAT+TGG + chr5.1:54073880-54073899 MS.gene43756:CDS 35.0%
TAACTTAAGATGGCAACACA+AGG - chr5.1:54074781-54074800 None:intergenic 35.0%
TATGATGAATTCAGAGGAAG+TGG + chr5.1:54074167-54074186 MS.gene43756:CDS 35.0%
TCATAGGAGTTAAAACCATG+AGG - chr5.1:54075191-54075210 None:intergenic 35.0%
TCTAACACTAGTAGCTCAAT+TGG - chr5.1:54074408-54074427 None:intergenic 35.0%
TCTGGTTATGATGAATTCAG+AGG + chr5.1:54074161-54074180 MS.gene43756:CDS 35.0%
TGAAATGATACCAGGAAAGA+CGG + chr5.1:54074013-54074032 MS.gene43756:CDS 35.0%
TTCACCCTTTACCTTAGATT+GGG + chr5.1:54074130-54074149 MS.gene43756:CDS 35.0%
! ACTAGTAGCTCAATTGGTTT+GGG - chr5.1:54074402-54074421 None:intergenic 35.0%
! TTCACCCAATCTAAGGTAAA+GGG - chr5.1:54074137-54074156 None:intergenic 35.0%
!! CAAGCCCTTTTTGAAGATTT+TGG - chr5.1:54075229-54075248 None:intergenic 35.0%
!! GTCTTGCATTTTAACCTCAT+AGG - chr5.1:54075207-54075226 None:intergenic 35.0%
!! TCCTGCAGATTATTTCTACT+AGG + chr5.1:54074810-54074829 MS.gene43756:intron 35.0%
!!! TCATGGTTTTAACTCCTATG+AGG + chr5.1:54075190-54075209 MS.gene43756:CDS 35.0%
AAGATAGCATGACCACACAA+TGG + chr5.1:54073910-54073929 MS.gene43756:CDS 40.0%
ACTTGTTCTTCAGAAGACAC+CGG + chr5.1:54075017-54075036 MS.gene43756:CDS 40.0%
AGAACTGTTCACCCAATCTA+AGG - chr5.1:54074144-54074163 None:intergenic 40.0%
CACCTTTGCCATACTTCTTT+AGG - chr5.1:54074836-54074855 None:intergenic 40.0%
CACTAGTAGCTCAATTGGTT+TGG - chr5.1:54074403-54074422 None:intergenic 40.0%
CTACTAGGCCTAAAGAAGTA+TGG + chr5.1:54074825-54074844 MS.gene43756:CDS 40.0%
CTTCACCCTTTACCTTAGAT+TGG + chr5.1:54074129-54074148 MS.gene43756:CDS 40.0%
GCCTAGTAGAAATAATCTGC+AGG - chr5.1:54074814-54074833 None:intergenic 40.0%
GCTATCTTCCATACACCAAT+TGG - chr5.1:54073898-54073917 None:intergenic 40.0%
GTAATTTAGACCTAGGAGGT+AGG + chr5.1:54074511-54074530 MS.gene43756:intron 40.0%
GTCTTCGTATCCACATAACA+TGG + chr5.1:54075256-54075275 MS.gene43756:CDS 40.0%
TTAGATTGGGTGAACAGTTC+TGG + chr5.1:54074143-54074162 MS.gene43756:CDS 40.0%
TTCATAAGACAGCTTTCAGG+AGG + chr5.1:54074930-54074949 MS.gene43756:CDS 40.0%
TTGTGTTCTTCTTCAGTCCA+TGG - chr5.1:54074245-54074264 None:intergenic 40.0%
TTTCAGGAGGAAAAGACAAG+AGG + chr5.1:54074943-54074962 MS.gene43756:CDS 40.0%
TTTGTGTGTATGATGCAGGA+GGG - chr5.1:54073872-54073891 None:intergenic 40.0%
! AATTGTTGTTCCTACCTCCT+AGG - chr5.1:54074524-54074543 None:intergenic 40.0%
! AGAAGTATGGCAAAGGTGAT+TGG + chr5.1:54074838-54074857 MS.gene43756:CDS 40.0%
! AGCTCATGAGCAAGTTTTCA+TGG + chr5.1:54075163-54075182 MS.gene43756:CDS 40.0%
! ATGAAAACTTGCTCATGAGC+TGG - chr5.1:54075164-54075183 None:intergenic 40.0%
! CAAGTTTTCATGGATCCTCA+TGG + chr5.1:54075173-54075192 MS.gene43756:CDS 40.0%
! GTTCACCCAATCTAAGGTAA+AGG - chr5.1:54074138-54074157 None:intergenic 40.0%
! TCTAAGGTAAAGGGTGAAGT+AGG - chr5.1:54074128-54074147 None:intergenic 40.0%
! TTTTGTGTGTATGATGCAGG+AGG - chr5.1:54073873-54073892 None:intergenic 40.0%
!! GAAGTAGGAGTGTTATAACC+AGG - chr5.1:54074113-54074132 None:intergenic 40.0%
!! GAGTTTTGTGTGTATGATGC+AGG - chr5.1:54073876-54073895 None:intergenic 40.0%
ATAAAGATACGCCGGATCGA+TGG + chr5.1:54073979-54073998 MS.gene43756:CDS 45.0%
ATGATACCAGGAAAGACGGT+CGG + chr5.1:54074017-54074036 MS.gene43756:CDS 45.0%
CATAGAATTCTGCGGTGAAG+TGG - chr5.1:54075051-54075070 None:intergenic 45.0%
CGGTGAAGTGGATCTATTAC+CGG - chr5.1:54075039-54075058 None:intergenic 45.0%
TGCTCATGAGCTGGATTGAA+AGG - chr5.1:54075155-54075174 None:intergenic 45.0%
TTGTTGCCGTCGAAGTTTGT+TGG - chr5.1:54075097-54075116 None:intergenic 45.0%
TTTAGTTAGAGCTCCTGAGC+AGG + chr5.1:54074202-54074221 MS.gene43756:CDS 45.0%
! CGACGGCAACAAATTTTCGT+TGG + chr5.1:54075105-54075124 MS.gene43756:CDS 45.0%
! CGGCAACAAATTTTCGTTGG+AGG + chr5.1:54075108-54075127 MS.gene43756:CDS 45.0%
! GCTACTTTATGCCATCGATC+CGG - chr5.1:54073993-54074012 None:intergenic 45.0%
! GGAGTGTTATAACCAGGAAC+TGG - chr5.1:54074107-54074126 None:intergenic 45.0%
! GGCCTAAAGAAGTATGGCAA+AGG + chr5.1:54074831-54074850 MS.gene43756:CDS 45.0%
! TGGTTTGGGCTAACGGTTAA+AGG - chr5.1:54074388-54074407 None:intergenic 45.0%
!! AAAGGAAGAAAGGAGTGCCA+TGG + chr5.1:54074225-54074244 MS.gene43756:CDS 45.0%
!! GCTCAATTGGTTTGGGCTAA+CGG - chr5.1:54074395-54074414 None:intergenic 45.0%
!! TATTTATAAATATATTTAGA+GGG + chr5.1:54074543-54074562 MS.gene43756:intron 5.0%
!! TTATTTATAAATATATTTAG+AGG + chr5.1:54074542-54074561 MS.gene43756:intron 5.0%
CCTGAGCAGGAAAGGAAGAA+AGG + chr5.1:54074215-54074234 MS.gene43756:CDS 50.0%
CCTTTCTTCCTTTCCTGCTC+AGG - chr5.1:54074218-54074237 None:intergenic 50.0%
GCAGCACCAACAAACTTCGA+CGG + chr5.1:54075088-54075107 MS.gene43756:CDS 50.0%
TTAGAGCTCCTGAGCAGGAA+AGG + chr5.1:54074207-54074226 MS.gene43756:CDS 50.0%
!! AAGTTTGTTGGTGCTGCTGC+TGG - chr5.1:54075085-54075104 None:intergenic 50.0%
!! CTCTTCTGGAGTCCATTGTG+TGG - chr5.1:54073925-54073944 None:intergenic 50.0%
!! GCATGGCTTGCTACTTGAGT+TGG - chr5.1:54074900-54074919 None:intergenic 50.0%
ACATCACCGACCGTCTTTCC+TGG - chr5.1:54074026-54074045 None:intergenic 55.0%
!! GCTGGGTTGATTCCAGTTCC+TGG + chr5.1:54074092-54074111 MS.gene43756:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 54073846 54075322 54073846 ID=MS.gene43756
chr5.1 mRNA 54073846 54075322 54073846 ID=MS.gene43756.t1;Parent=MS.gene43756
chr5.1 exon 54073846 54074264 54073846 ID=MS.gene43756.t1.exon1;Parent=MS.gene43756.t1
chr5.1 CDS 54073846 54074264 54073846 ID=cds.MS.gene43756.t1;Parent=MS.gene43756.t1
chr5.1 exon 54074818 54075322 54074818 ID=MS.gene43756.t1.exon2;Parent=MS.gene43756.t1
chr5.1 CDS 54074818 54075322 54074818 ID=cds.MS.gene43756.t1;Parent=MS.gene43756.t1
Gene Sequence

>MS.gene43756

ATGAAGTGGGAAATGGAAGTTCTCCCTCCTGCATCATACACACAAAACTCCAATTGGTGTATGGAAGATAGCATGACCACACAATGGACTCCAGAAGAGAACAAAATATTTGAAAATGCTCTTGCAGTTCATGATAAAGATACGCCGGATCGATGGCATAAAGTAGCTGAAATGATACCAGGAAAGACGGTCGGTGATGTGATGAGACAATATAAGGAATTAGAAGATGATGTTTGTAATATAGAAGCTGGGTTGATTCCAGTTCCTGGTTATAACACTCCTACTTCACCCTTTACCTTAGATTGGGTGAACAGTTCTGGTTATGATGAATTCAGAGGAAGTGGAAAGAGATCTTCTTTAGTTAGAGCTCCTGAGCAGGAAAGGAAGAAAGGAGTGCCATGGACTGAAGAAGAACACAAGTAAATTTTTTACCTTTTGTATTTGTTTTGTTATTATTGTTCTTATTGTTTAGAAAGAAAAATAATAGTATATTAGTTGTTATGTTAGTATTTTTTCTTTACTAGGGTTTAAATTCTAAACCTTTAACCGTTAGCCCAAACCAATTGAGCTACTAGTGTTAGATAGATATAGAAGAATGTATGTATGTATGTTTGAAACATTGTGAGCTTAAGAAAGTATAGGAATAATGGATGGAATTTACAAAGGTAATTTAGACCTAGGAGGTAGGAACAACAATTATTTATAAATATATTTAGAGGGTTGAATATTTTCATTTTTCAGGTTGTAATAAGGTTTTTTAGCTTTCACTTTAAGGCTAGTGATAAGTCTAAATAATGCTTACAAAATGTAGACAATCCTTACCTTACAAGATGATTTTGTAAAGTCGAGTTAGACCAAAATCCTCGAATATCATCTTAAGTTAGAATAGGTTATGATATAAGTTAGTTTTGTAGGGCTGATGTAGACTAAAACCTTGTGTTGCCATCTTAAGTTAGAATATATTTCCTGCAGATTATTTCTACTAGGCCTAAAGAAGTATGGCAAAGGTGATTGGAGAAATATTTCGCGCAATTTCGTCATCACAAGAACACCAACTCAAGTAGCAAGCCATGCTCAAAAGTATTTCATAAGACAGCTTTCAGGAGGAAAAGACAAGAGGAGAGCAAGCATACATGACATAACAACAGTTAATCTTTCAGAAAAAATTGGAACTTGTTCTTCAGAAGACACCGGTAATAGATCCACTTCACCGCAGAATTCTATGTTACTTTCACACCAGCAGCAGCACCAACAAACTTCGACGGCAACAAATTTTCGTTGGAGGAATGATCAACAAAATGCTATGCCTTTCAATCCAGCTCATGAGCAAGTTTTCATGGATCCTCATGGTTTTAACTCCTATGAGGTTAAAATGCAAGACCAAAATCTTCAAAAAGGGCTTGTTCATGAGTCTTCGTATCCACATAACATGGTTTTTCAAATGCAACATTCATCACAACATTATTCACATGCATAA
Protein sequence

>MS.gene43756.t1

MKWEMEVLPPASYTQNSNWCMEDSMTTQWTPEENKIFENALAVHDKDTPDRWHKVAEMIPGKTVGDVMRQYKELEDDVCNIEAGLIPVPGYNTPTSPFTLDWVNSSGYDEFRGSGKRSSLVRAPEQERKKGVPWTEEEHKLFLLGLKKYGKGDWRNISRNFVITRTPTQVASHAQKYFIRQLSGGKDKRRASIHDITTVNLSEKIGTCSSEDTGNRSTSPQNSMLLSHQQQHQQTSTATNFRWRNDQQNAMPFNPAHEQVFMDPHGFNSYEVKMQDQNLQKGLVHESSYPHNMVFQMQHSSQHYSHA