Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43810.t1 | XP_003615675.1 | 97.6 | 207 | 5 | 0 | 1 | 207 | 156 | 362 | 2.80E-102 | 381.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43810.t1 | F4IQJ2 | 50.5 | 222 | 95 | 4 | 1 | 207 | 153 | 374 | 1.7e-44 | 180.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43810.t1 | G7KE89 | 97.6 | 207 | 5 | 0 | 1 | 207 | 156 | 362 | 2.0e-102 | 381.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene43810 | 0.818618 | 1.78E-52 | -1.69E-46 |
| MS.gene049873 | MS.gene43810 | 0.826563 | 2.53E-54 | -1.69E-46 |
| MS.gene052613 | MS.gene43810 | 0.805482 | 1.31E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene43810 | 0.80643 | 8.26E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene43810 | 0.803057 | 4.18E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene43810 | 0.832634 | 8.45E-56 | -1.69E-46 |
| MS.gene061040 | MS.gene43810 | -0.815789 | 7.72E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43810.t1 | MTR_5g070860 | 97.585 | 207 | 5 | 0 | 1 | 207 | 156 | 362 | 9.66e-146 | 410 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43810.t1 | AT2G18410 | 50.476 | 210 | 89 | 4 | 1 | 195 | 153 | 362 | 2.33e-60 | 193 |
| MS.gene43810.t1 | AT2G18410 | 44.030 | 134 | 65 | 3 | 1 | 124 | 153 | 286 | 1.27e-27 | 106 |
Find 0 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTAGGATTTTAAATTG+TGG | + | 37956:1442-1461 | MS.gene43810:intron | 15.0% |
| !!! | AATTTAGGATTTTAAATTGT+GGG | + | 37956:1443-1462 | MS.gene43810:intron | 15.0% |
| !! | AAAATATATAATCTCACCTA+GGG | - | 37956:2121-2140 | MS.gene43810:intergenic | 20.0% |
| !! | AGTAGATAATATTTATGTCA+CGG | + | 37956:827-846 | MS.gene43810:intron | 20.0% |
| !! | ATATATCTAAATCATCATCT+GGG | - | 37956:2182-2201 | MS.gene43810:intergenic | 20.0% |
| !! | TTCAACCTATTATTTATTTG+CGG | - | 37956:1823-1842 | MS.gene43810:intergenic | 20.0% |
| !!! | ATTCAAAGGTATTTTTTCTA+AGG | + | 37956:1352-1371 | MS.gene43810:intron | 20.0% |
| !!! | ATTTGCTCTTATTTTGAATA+CGG | + | 37956:702-721 | MS.gene43810:intron | 20.0% |
| !!! | GATCAAATTTCAAGTATATT+TGG | + | 37956:905-924 | MS.gene43810:intron | 20.0% |
| !!! | GTTGAAACTTTGTTTTTAAT+AGG | + | 37956:1837-1856 | MS.gene43810:intron | 20.0% |
| !!! | TGTTATATGTAATTTGCAAT+TGG | + | 37956:1581-1600 | MS.gene43810:intron | 20.0% |
| ! | ATTGACCGCAAATAAATAAT+AGG | + | 37956:1815-1834 | MS.gene43810:intron | 25.0% |
| ! | CATATATCTAAATCATCATC+TGG | - | 37956:2183-2202 | MS.gene43810:intergenic | 25.0% |
| ! | CTACCATCATAAATTTGAAT+CGG | - | 37956:2033-2052 | MS.gene43810:intergenic | 25.0% |
| ! | GAAAATATATAATCTCACCT+AGG | - | 37956:2122-2141 | MS.gene43810:intergenic | 25.0% |
| ! | GAAATGAAAATTTAGATGCT+AGG | + | 37956:1374-1393 | MS.gene43810:intron | 25.0% |
| ! | GTAGATAATATTTATGTCAC+GGG | + | 37956:828-847 | MS.gene43810:intron | 25.0% |
| ! | GTGCTTCTAACATTAAATTT+AGG | + | 37956:1428-1447 | MS.gene43810:intron | 25.0% |
| ! | TACAAATCTATATCATACCT+TGG | - | 37956:1933-1952 | MS.gene43810:intergenic | 25.0% |
| ! | TTCTTTATCGTGAACTTATT+TGG | + | 37956:1121-1140 | MS.gene43810:intron | 25.0% |
| !! | ATATGCTCTCTTTTATAAAG+GGG | - | 37956:1331-1350 | MS.gene43810:intergenic | 25.0% |
| !! | GATATGCTCTCTTTTATAAA+GGG | - | 37956:1332-1351 | MS.gene43810:intergenic | 25.0% |
| !! | GGTAAAATTTTTCTCCAATA+AGG | - | 37956:1035-1054 | MS.gene43810:intergenic | 25.0% |
| !! | TCTGACATTAAATTTTCCTT+GGG | - | 37956:1056-1075 | MS.gene43810:intergenic | 25.0% |
| !! | TGATATGCTCTCTTTTATAA+AGG | - | 37956:1333-1352 | MS.gene43810:intergenic | 25.0% |
| !! | TTTCAATCAAAGCATCAAAT+AGG | - | 37956:1402-1421 | MS.gene43810:intergenic | 25.0% |
| AGCAAAAATAAGATCTCTTC+AGG | - | 37956:1303-1322 | MS.gene43810:intergenic | 30.0% | |
| ATAATATTTATGTCACGGGT+TGG | + | 37956:832-851 | MS.gene43810:intron | 30.0% | |
| CAAAATAAGAGCAAATACCA+TGG | - | 37956:698-717 | MS.gene43810:intergenic | 30.0% | |
| GAATTGCTAATCTTCAAAAC+TGG | + | 37956:1143-1162 | MS.gene43810:intron | 30.0% | |
| GATTTAAACACCGTAAGAAA+TGG | - | 37956:1962-1981 | MS.gene43810:intergenic | 30.0% | |
| TATCGTGTGTGTATATAAGA+AGG | - | 37956:1679-1698 | MS.gene43810:intergenic | 30.0% | |
| TGTATTTGAGCCATTTCTTA+CGG | + | 37956:1949-1968 | MS.gene43810:intron | 30.0% | |
| ! | AAAGAGAGCATATCATTCAA+AGG | + | 37956:1338-1357 | MS.gene43810:intron | 30.0% |
| ! | GAAAAGTTTAAAGTTGAAGC+AGG | + | 37956:1198-1217 | MS.gene43810:CDS | 30.0% |
| ! | GTCTGACATTAAATTTTCCT+TGG | - | 37956:1057-1076 | MS.gene43810:intergenic | 30.0% |
| ! | TATGCTCTCTTTTATAAAGG+GGG | - | 37956:1330-1349 | MS.gene43810:intergenic | 30.0% |
| ! | TTGGAGAAAAATTTTACCCA+AGG | + | 37956:1037-1056 | MS.gene43810:CDS | 30.0% |
| ACCTCTTTCAAAGGGTAAAA+AGG | + | 37956:2086-2105 | MS.gene43810:CDS | 35.0% | |
| ACTGTACTGTACAATCAACT+AGG | - | 37956:1538-1557 | MS.gene43810:intergenic | 35.0% | |
| ATCACACACCATATCTTGTT+TGG | - | 37956:1776-1795 | MS.gene43810:intergenic | 35.0% | |
| TCTGCTGTAATGATCCAAAT+CGG | - | 37956:1469-1488 | MS.gene43810:intergenic | 35.0% | |
| TCTTTCAAAGGGTAAAAAGG+AGG | + | 37956:2089-2108 | MS.gene43810:CDS | 35.0% | |
| TTTCTCCAATAAGGAGTTCT+CGG | - | 37956:1026-1045 | MS.gene43810:intergenic | 35.0% | |
| ! | AAGTTTAAAGTTGAAGCAGG+AGG | + | 37956:1201-1220 | MS.gene43810:CDS | 35.0% |
| ! | ATGCTCTCTTTTATAAAGGG+GGG | - | 37956:1329-1348 | MS.gene43810:intergenic | 35.0% |
| ! | TGCTTAAGCTCTTCTCTTTT+AGG | + | 37956:1997-2016 | MS.gene43810:intron | 35.0% |
| ! | TTTTAAATTGTGGGCCGATT+TGG | + | 37956:1452-1471 | MS.gene43810:intron | 35.0% |
| !! | AGCTCTTCTCTTTTAGGAAA+TGG | + | 37956:2003-2022 | MS.gene43810:intron | 35.0% |
| !! | ATCATACCTTGGTGTTCAAA+AGG | - | 37956:1922-1941 | MS.gene43810:intergenic | 35.0% |
| !! | GCACCGATTCAAATTTATGA+TGG | + | 37956:2027-2046 | MS.gene43810:CDS | 35.0% |
| !! | TCCTTTTTACCCTTTGAAAG+AGG | - | 37956:2090-2109 | MS.gene43810:intergenic | 35.0% |
| AAATGGACGAGTTAGAGTGA+CGG | + | 37956:1087-1106 | MS.gene43810:CDS | 40.0% | |
| ACAATTCAATCTCCAGCTGT+CGG | + | 37956:1861-1880 | MS.gene43810:CDS | 40.0% | |
| ACTAAGTGCTTAGCATGTGT+AGG | - | 37956:1649-1668 | MS.gene43810:intergenic | 40.0% | |
| ACTCTCACATTCTAGACTTC+TGG | - | 37956:793-812 | MS.gene43810:intergenic | 40.0% | |
| ATTGCATTCTGACCTTCATG+AGG | + | 37956:931-950 | MS.gene43810:CDS | 40.0% | |
| CACAGTCACAAACAGTTGTA+AGG | + | 37956:1737-1756 | MS.gene43810:intron | 40.0% | |
| CTGTACAATCAACTAGGTTG+CGG | - | 37956:1532-1551 | MS.gene43810:intergenic | 40.0% | |
| GACAGAGACCAAACAAGATA+TGG | + | 37956:1765-1784 | MS.gene43810:intron | 40.0% | |
| GGAAACTAAAGACTGACCTT+CGG | - | 37956:1282-1301 | MS.gene43810:intergenic | 40.0% | |
| TTCTTCCGAGAACTCCTTAT+TGG | + | 37956:1018-1037 | MS.gene43810:CDS | 40.0% | |
| ACTTCTGCTTCAGCAGAAGA+TGG | + | 37956:1234-1253 | MS.gene43810:CDS | 45.0% | |
| ATTCTGACCTTCATGAGGAG+AGG | + | 37956:936-955 | MS.gene43810:CDS | 45.0% | |
| GCAACTACATCTGACTGTGA+GGG | - | 37956:1501-1520 | MS.gene43810:intergenic | 45.0% | |
| GTCAGACTCAAACGCAGAAA+TGG | + | 37956:1070-1089 | MS.gene43810:CDS | 45.0% | |
| TCTGGGTCTTCATCAGAATC+TGG | - | 37956:2165-2184 | MS.gene43810:intergenic | 45.0% | |
| TCTTGAGTACATGTCCTCCA+TGG | + | 37956:970-989 | MS.gene43810:CDS | 45.0% | |
| TGATTCTACACTAGCCACCA+TGG | - | 37956:990-1009 | MS.gene43810:intergenic | 45.0% | |
| TGCAACTACATCTGACTGTG+AGG | - | 37956:1502-1521 | MS.gene43810:intergenic | 45.0% | |
| TTCTGACCTTCATGAGGAGA+GGG | + | 37956:937-956 | MS.gene43810:CDS | 45.0% | |
| ! | GTGCTTCCTTTTGAACACCA+AGG | + | 37956:1913-1932 | MS.gene43810:CDS | 45.0% |
| !! | GGAGAAGGAGCAAGTTGATA+GGG | + | 37956:1882-1901 | MS.gene43810:CDS | 45.0% |
| AAAAAGGAGGACTCAGCCCT+AGG | + | 37956:2102-2121 | MS.gene43810:CDS | 50.0% | |
| AGCATGTGTAGGCATGGCAT+CGG | - | 37956:1638-1657 | MS.gene43810:intergenic | 50.0% | |
| CTAAGCAATCTGCGTAGCCA+TGG | + | 37956:678-697 | MS.gene43810:CDS | 50.0% | |
| CTCACTGCTGCTCTCTTCAA+GGG | - | 37956:2061-2080 | MS.gene43810:intergenic | 50.0% | |
| CTTGAAGAGAGCAGCAGTGA+GGG | + | 37956:2060-2079 | MS.gene43810:CDS | 50.0% | |
| GAAGATGGAACAACCGTTGC+AGG | + | 37956:1249-1268 | MS.gene43810:CDS | 50.0% | |
| GGCTACGCAGATTGCTTAGA+AGG | - | 37956:677-696 | MS.gene43810:intergenic | 50.0% | |
| GTGCTTAGCATGTGTAGGCA+TGG | - | 37956:1644-1663 | MS.gene43810:intergenic | 50.0% | |
| TGAGTACATGTCCTCCATGG+TGG | + | 37956:973-992 | MS.gene43810:CDS | 50.0% | |
| TTCTACACTAGCCACCATGG+AGG | - | 37956:987-1006 | MS.gene43810:intergenic | 50.0% | |
| TTGAAGAGAGCAGCAGTGAG+GGG | + | 37956:2061-2080 | MS.gene43810:CDS | 50.0% | |
| !! | CGGAGAAGGAGCAAGTTGAT+AGG | + | 37956:1881-1900 | MS.gene43810:CDS | 50.0% |
| !! | GGAGCAAGTTGATAGGGCAA+AGG | + | 37956:1888-1907 | MS.gene43810:CDS | 50.0% |
| CAATCTCCAGCTGTCGGAGA+AGG | + | 37956:1867-1886 | MS.gene43810:CDS | 55.0% | |
| CCTCACTGCTGCTCTCTTCA+AGG | - | 37956:2062-2081 | MS.gene43810:intergenic | 55.0% | |
| CCTTGAAGAGAGCAGCAGTG+AGG | + | 37956:2059-2078 | MS.gene43810:CDS | 55.0% | |
| CTTCGGCAGTAAGCCTGCAA+CGG | - | 37956:1265-1284 | MS.gene43810:intergenic | 55.0% | |
| GCATCTTTGCAGTCAGTCGC+AGG | + | 37956:651-670 | MS.gene43810:CDS | 55.0% | |
| TGAAGAGAGCAGCAGTGAGG+GGG | + | 37956:2062-2081 | MS.gene43810:CDS | 55.0% | |
| !! | GAGGGGGCACCTCTTTCAAA+GGG | + | 37956:2078-2097 | MS.gene43810:CDS | 55.0% |
| !! | TGAGGGGGCACCTCTTTCAA+AGG | + | 37956:2077-2096 | MS.gene43810:CDS | 55.0% |
| CGTTGCAGGCTTACTGCCGA+AGG | + | 37956:1263-1282 | MS.gene43810:CDS | 60.0% | |
| CTGCAGCCCTCTCCTCATGA+AGG | - | 37956:946-965 | MS.gene43810:intergenic | 60.0% | |
| CTTGCTCCTTCTCCGACAGC+TGG | - | 37956:1876-1895 | MS.gene43810:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 37956 | gene | 630 | 2203 | 630 | ID=MS.gene43810 |
| 37956 | mRNA | 630 | 2203 | 630 | ID=MS.gene43810.t1;Parent=MS.gene43810 |
| 37956 | exon | 630 | 699 | 630 | ID=MS.gene43810.t1.exon1;Parent=MS.gene43810.t1 |
| 37956 | CDS | 630 | 699 | 630 | ID=cds.MS.gene43810.t1;Parent=MS.gene43810.t1 |
| 37956 | exon | 906 | 1108 | 906 | ID=MS.gene43810.t1.exon2;Parent=MS.gene43810.t1 |
| 37956 | CDS | 906 | 1108 | 906 | ID=cds.MS.gene43810.t1;Parent=MS.gene43810.t1 |
| 37956 | exon | 1195 | 1284 | 1195 | ID=MS.gene43810.t1.exon3;Parent=MS.gene43810.t1 |
| 37956 | CDS | 1195 | 1284 | 1195 | ID=cds.MS.gene43810.t1;Parent=MS.gene43810.t1 |
| 37956 | exon | 1859 | 1934 | 1859 | ID=MS.gene43810.t1.exon4;Parent=MS.gene43810.t1 |
| 37956 | CDS | 1859 | 1934 | 1859 | ID=cds.MS.gene43810.t1;Parent=MS.gene43810.t1 |
| 37956 | exon | 2019 | 2203 | 2019 | ID=MS.gene43810.t1.exon5;Parent=MS.gene43810.t1 |
| 37956 | CDS | 2019 | 2203 | 2019 | ID=cds.MS.gene43810.t1;Parent=MS.gene43810.t1 |
| Gene Sequence |
| Protein sequence |
