AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052613


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 11 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGTCTCCTCAGGTTTCTA+TGG 0.325287 3.1:-61881009 MS.gene052613:intron
GGAGAGAGCTCCAAGGTAAC+AGG 0.385509 3.1:+61880973 None:intergenic
ACATTAATAAGTACTTATAC+TGG 0.437619 3.1:+61880925 None:intergenic
TTTGAAGGTACCGCGGAAAA+CGG 0.439053 3.1:-61882049 None:intergenic
TGATCATTGTCCTGTTACCT+TGG 0.459781 3.1:-61880983 MS.gene052613:CDS
GCATGTGAAATGCATGGAAA+AGG 0.488169 3.1:-61881975 MS.gene052613:CDS
CATGTGAAATGCATGGAAAA+GGG 0.549839 3.1:-61881974 MS.gene052613:CDS
TAGTTCCAACTCTCAAAATG+AGG 0.586067 3.1:-61880950 MS.gene052613:CDS
ATTGTTGCATGTGAAATGCA+TGG 0.619220 3.1:-61881981 MS.gene052613:CDS
GGAAAAGGGATAGAGTTACA+AGG 0.646371 3.1:-61881960 MS.gene052613:intron
GGAACTAGGAGAGAGCTCCA+AGG 0.654013 3.1:+61880966 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTTAATTAATTTATTAT+CGG + chr3.1:61881281-61881300 None:intergenic 0.0%
!!! TTTTAATTTATTTATTTTTT+TGG + chr3.1:61881564-61881583 None:intergenic 0.0%
!! ATTGAAAATGATAATAACAA+TGG + chr3.1:61881912-61881931 None:intergenic 15.0%
!!! AAAATTTGATATTCGTTTTT+AGG - chr3.1:61881085-61881104 MS.gene052613:intron 15.0%
!!! AAATTTGATATTCGTTTTTA+GGG - chr3.1:61881086-61881105 MS.gene052613:intron 15.0%
!!! ATGATTTATGAAATTTTGAA+TGG - chr3.1:61881478-61881497 MS.gene052613:intron 15.0%
!! ATAAATCATACATAATCCAT+AGG + chr3.1:61881467-61881486 None:intergenic 20.0%
!! TTTAAGTAATAAGATGTCTA+AGG + chr3.1:61881313-61881332 None:intergenic 20.0%
!!! AATTTGATATTCGTTTTTAG+GGG - chr3.1:61881087-61881106 MS.gene052613:intron 20.0%
!!! GTTTAAAAGTTGAAGTTTTT+TGG - chr3.1:61881514-61881533 MS.gene052613:intron 20.0%
!!! TTTTAACTTAGATAAGCTAA+AGG + chr3.1:61881761-61881780 None:intergenic 20.0%
! ACATCATAAAACATATCCTA+TGG - chr3.1:61881448-61881467 MS.gene052613:intron 25.0%
! TACCCATTATTATATGGATA+CGG - chr3.1:61881215-61881234 MS.gene052613:intron 25.0%
! TTTGACTACCCATTATTATA+TGG - chr3.1:61881209-61881228 MS.gene052613:intron 25.0%
!! AATTTTGAATGGATGATGTA+TGG - chr3.1:61881489-61881508 MS.gene052613:intron 25.0%
ATCAATGAAGAATCTTAGGT+GGG + chr3.1:61881365-61881384 None:intergenic 30.0%
GAACCATATACATAAAAGGA+GGG + chr3.1:61881736-61881755 None:intergenic 30.0%
GGTTATCAATGAAGAATCTT+AGG + chr3.1:61881369-61881388 None:intergenic 30.0%
GTCCGTATCCATATAATAAT+GGG + chr3.1:61881220-61881239 None:intergenic 30.0%
GTTGTATCTAACCTGTTTAA+TGG - chr3.1:61881824-61881843 MS.gene052613:intron 30.0%
TAACTTAGATAAGCTAAAGG+AGG + chr3.1:61881758-61881777 None:intergenic 30.0%
TATCAATGAAGAATCTTAGG+TGG + chr3.1:61881366-61881385 None:intergenic 30.0%
TATCCACGGGTATAATAAAT+GGG - chr3.1:61881184-61881203 MS.gene052613:intron 30.0%
TGTCCGTATCCATATAATAA+TGG + chr3.1:61881221-61881240 None:intergenic 30.0%
TTATTATATGGATACGGACA+CGG - chr3.1:61881221-61881240 MS.gene052613:intron 30.0%
! CGGTAAAATTTGCTTCAATA+AGG + chr3.1:61881342-61881361 None:intergenic 30.0%
! GTTTTATGATGTGAATGAAG+TGG + chr3.1:61881440-61881459 None:intergenic 30.0%
AAAAAATTCATGGGTACCCT+GGG - chr3.1:61881580-61881599 MS.gene052613:intron 35.0%
ATAAAAGGAGGGATGTCAAT+GGG + chr3.1:61881725-61881744 None:intergenic 35.0%
ATACCCATTTATTATACCCG+TGG + chr3.1:61881190-61881209 None:intergenic 35.0%
ATTGTTGCATGTGAAATGCA+TGG - chr3.1:61881000-61881019 MS.gene052613:intron 35.0%
CATGTGAAATGCATGGAAAA+GGG - chr3.1:61881007-61881026 MS.gene052613:intron 35.0%
CGGGTATCATATTTATCCAA+CGG - chr3.1:61881661-61881680 MS.gene052613:intron 35.0%
GGAACCATATACATAAAAGG+AGG + chr3.1:61881737-61881756 None:intergenic 35.0%
GGAGGAACCATATACATAAA+AGG + chr3.1:61881740-61881759 None:intergenic 35.0%
GGGTATCATATTTATCCAAC+GGG - chr3.1:61881662-61881681 MS.gene052613:intron 35.0%
GGTATCATATTTATCCAACG+GGG - chr3.1:61881663-61881682 MS.gene052613:intron 35.0%
GTATCCACGGGTATAATAAA+TGG - chr3.1:61881183-61881202 MS.gene052613:intron 35.0%
TAAAAAATTCATGGGTACCC+TGG - chr3.1:61881579-61881598 MS.gene052613:intron 35.0%
TAATGGCTACCCCAAAAATT+AGG - chr3.1:61881841-61881860 MS.gene052613:intron 35.0%
TAGTTCCAACTCTCAAAATG+AGG - chr3.1:61882031-61882050 MS.gene052613:CDS 35.0%
TGATGTGAATGAAGTGGAAA+CGG + chr3.1:61881434-61881453 None:intergenic 35.0%
TTTGATTGTACTCATACCCA+CGG - chr3.1:61881245-61881264 MS.gene052613:intron 35.0%
! ACAGCAAAGCCTAATTTTTG+GGG + chr3.1:61881853-61881872 None:intergenic 35.0%
! CATCCCTCCTTTTATGTATA+TGG - chr3.1:61881730-61881749 MS.gene052613:intron 35.0%
! GACAGCAAAGCCTAATTTTT+GGG + chr3.1:61881854-61881873 None:intergenic 35.0%
! TGACAGCAAAGCCTAATTTT+TGG + chr3.1:61881855-61881874 None:intergenic 35.0%
! TTCTTTTCGTGTGATGATAC+TGG - chr3.1:61881049-61881068 MS.gene052613:intron 35.0%
!! TCATTTTGAGAGTTGGAACT+AGG + chr3.1:61882032-61882051 None:intergenic 35.0%
!! TTGATATTCGTTTTTAGGGG+TGG - chr3.1:61881090-61881109 MS.gene052613:intron 35.0%
!! TTGTGGTAATTGGTGTTGTT+GGG - chr3.1:61881802-61881821 MS.gene052613:intron 35.0%
!! TTTGTGGTAATTGGTGTTGT+TGG - chr3.1:61881801-61881820 MS.gene052613:intron 35.0%
!!! GCTGTCATTTTCATATGCAT+GGG - chr3.1:61881868-61881887 MS.gene052613:intron 35.0%
!!! TGCTGTCATTTTCATATGCA+TGG - chr3.1:61881867-61881886 MS.gene052613:intron 35.0%
AATGAAGAATCTTAGGTGGG+CGG + chr3.1:61881362-61881381 None:intergenic 40.0%
ATCACTTCCATAGAAACCTG+AGG + chr3.1:61881982-61882001 None:intergenic 40.0%
ATCATATTTATCCAACGGGG+CGG - chr3.1:61881666-61881685 MS.gene052613:intron 40.0%
ATTACCACAAAGTTGCACTC+TGG + chr3.1:61881792-61881811 None:intergenic 40.0%
CATAAAAGGAGGGATGTCAA+TGG + chr3.1:61881726-61881745 None:intergenic 40.0%
GAGTGCAACTTTGTGGTAAT+TGG - chr3.1:61881792-61881811 MS.gene052613:intron 40.0%
GCATGTGAAATGCATGGAAA+AGG - chr3.1:61881006-61881025 MS.gene052613:intron 40.0%
GGAAAAGGGATAGAGTTACA+AGG - chr3.1:61881021-61881040 MS.gene052613:intron 40.0%
TGATCATTGTCCTGTTACCT+TGG - chr3.1:61881998-61882017 MS.gene052613:CDS 40.0%
TTGATTGTACTCATACCCAC+GGG - chr3.1:61881246-61881265 MS.gene052613:intron 40.0%
!! TGGATCCTCATTTTGAGAGT+TGG + chr3.1:61882039-61882058 None:intergenic 40.0%
!!! TTTTGGGGTAGCCATTAAAC+AGG + chr3.1:61881838-61881857 None:intergenic 40.0%
AACCTGAGGAGACAACAACA+AGG + chr3.1:61881968-61881987 None:intergenic 45.0%
ATCATGCTATCCGTACCCAT+GGG - chr3.1:61881697-61881716 MS.gene052613:intron 45.0%
GAATCCAGAGTGCAACTTTG+TGG - chr3.1:61881785-61881804 MS.gene052613:intron 45.0%
GTTGTCTCCTCAGGTTTCTA+TGG - chr3.1:61881972-61881991 MS.gene052613:CDS 45.0%
TATCATGCTATCCGTACCCA+TGG - chr3.1:61881696-61881715 MS.gene052613:intron 45.0%
TCATATTTATCCAACGGGGC+GGG - chr3.1:61881667-61881686 MS.gene052613:intron 45.0%
TCCGTGGGTAAAATCCATCA+CGG - chr3.1:61881137-61881156 MS.gene052613:intron 45.0%
! CTATTCTCATCCGTTTTCCG+CGG + chr3.1:61880945-61880964 None:intergenic 45.0%
!! AAAAAATTCTAAATAAAAAT+AGG + chr3.1:61881390-61881409 None:intergenic 5.0%
!! AAATAAATTAAAAAATTCAT+GGG - chr3.1:61881571-61881590 MS.gene052613:intron 5.0%
!! AATTTAATTAATTTATTATC+GGG + chr3.1:61881280-61881299 None:intergenic 5.0%
!! TAAATAAATTAAAAAATTCA+TGG - chr3.1:61881570-61881589 MS.gene052613:intron 5.0%
CATATTTATCCAACGGGGCG+GGG - chr3.1:61881668-61881687 MS.gene052613:intron 50.0%
CCCTCGTGGTATCCACTATA+CGG + chr3.1:61881628-61881647 None:intergenic 50.0%
CCGTGGGTAAAATCCATCAC+GGG - chr3.1:61881138-61881157 MS.gene052613:intron 50.0%
CCTCGTGGTATCCACTATAC+GGG + chr3.1:61881627-61881646 None:intergenic 50.0%
GCAAAAATACCCATGCCCAC+GGG - chr3.1:61881112-61881131 MS.gene052613:intron 50.0%
GGATGTCAATGGGTACCCAT+GGG + chr3.1:61881715-61881734 None:intergenic 50.0%
GGCAAAAATACCCATGCCCA+CGG - chr3.1:61881111-61881130 MS.gene052613:intron 50.0%
GGTAAAATCCATCACGGGCA+CGG - chr3.1:61881143-61881162 MS.gene052613:intron 50.0%
GGTAGCTAAACGGGTATCCA+CGG - chr3.1:61881170-61881189 MS.gene052613:intron 50.0%
GTAAAATCCATCACGGGCAC+GGG - chr3.1:61881144-61881163 MS.gene052613:intron 50.0%
GTAGCTAAACGGGTATCCAC+GGG - chr3.1:61881171-61881190 MS.gene052613:intron 50.0%
TAAAATCCATCACGGGCACG+GGG - chr3.1:61881145-61881164 MS.gene052613:intron 50.0%
TATCGGGTATGAGTACCCGT+GGG + chr3.1:61881264-61881283 None:intergenic 50.0%
TCAATGGGTACCCATGGGTA+CGG + chr3.1:61881710-61881729 None:intergenic 50.0%
TTATCGGGTATGAGTACCCG+TGG + chr3.1:61881265-61881284 None:intergenic 50.0%
! ATTTTACCCACGGATACCCG+TGG + chr3.1:61881131-61881150 None:intergenic 50.0%
! TAGTGGATACCACGAGGGTA+CGG - chr3.1:61881630-61881649 MS.gene052613:intron 50.0%
! TTTTACCCACGGATACCCGT+GGG + chr3.1:61881130-61881149 None:intergenic 50.0%
!! CCGTATAGTGGATACCACGA+GGG - chr3.1:61881625-61881644 MS.gene052613:intron 50.0%
!! GACCTTGTTGTTGTCTCCTC+AGG - chr3.1:61881963-61881982 MS.gene052613:CDS 50.0%
!!! CCCGTGATGGATTTTACCCA+CGG + chr3.1:61881141-61881160 None:intergenic 50.0%
ATCCACTATACGGGTACCCG+CGG + chr3.1:61881618-61881637 None:intergenic 55.0%
CTGGGATACCTCAATACCCG+CGG - chr3.1:61881598-61881617 MS.gene052613:intron 55.0%
GGAACTAGGAGAGAGCTCCA+AGG + chr3.1:61882018-61882037 None:intergenic 55.0%
GGAGAGAGCTCCAAGGTAAC+AGG + chr3.1:61882011-61882030 None:intergenic 55.0%
GGGATGTCAATGGGTACCCA+TGG + chr3.1:61881716-61881735 None:intergenic 55.0%
TGGGATACCTCAATACCCGC+GGG - chr3.1:61881599-61881618 MS.gene052613:intron 55.0%
! AGTGGATACCACGAGGGTAC+GGG - chr3.1:61881631-61881650 MS.gene052613:intron 55.0%
!! CCCGTATAGTGGATACCACG+AGG - chr3.1:61881624-61881643 MS.gene052613:intron 55.0%
!! GCGGGTATTGAGGTATCCCA+GGG + chr3.1:61881599-61881618 None:intergenic 55.0%
TCCACTATACGGGTACCCGC+GGG + chr3.1:61881617-61881636 None:intergenic 60.0%
! GTGGATACCACGAGGGTACG+GGG - chr3.1:61881632-61881651 MS.gene052613:intron 60.0%
!! CGCGGGTATTGAGGTATCCC+AGG + chr3.1:61881600-61881619 None:intergenic 60.0%
AATCCATCACGGGCACGGGG+CGG - chr3.1:61881148-61881167 MS.gene052613:intron 65.0%
ACCCGCGGGTACCCGTATAG+TGG - chr3.1:61881613-61881632 MS.gene052613:intron 65.0%
CACGGGGCGGGTAGCTAAAC+GGG - chr3.1:61881161-61881180 MS.gene052613:intron 65.0%
CCACGGATACCCGTGGGCAT+GGG + chr3.1:61881124-61881143 None:intergenic 65.0%
CCATGCCCACGGGTATCCGT+GGG - chr3.1:61881122-61881141 MS.gene052613:intron 65.0%
GCACGGGGCGGGTAGCTAAA+CGG - chr3.1:61881160-61881179 MS.gene052613:intron 65.0%
TTATCCAACGGGGCGGGGAC+TGG - chr3.1:61881673-61881692 MS.gene052613:intron 65.0%
! ACGGGTACCCGCGGGTATTG+AGG + chr3.1:61881609-61881628 None:intergenic 65.0%
! GATACCACGAGGGTACGGGG+CGG - chr3.1:61881635-61881654 MS.gene052613:intron 65.0%
ATCCATCACGGGCACGGGGC+GGG - chr3.1:61881149-61881168 MS.gene052613:intron 70.0%
CCCACGGATACCCGTGGGCA+TGG + chr3.1:61881125-61881144 None:intergenic 70.0%
CCCATGCCCACGGGTATCCG+TGG - chr3.1:61881121-61881140 MS.gene052613:intron 70.0%
GATACCAGTCCCCGCCCCGT+TGG + chr3.1:61881680-61881699 None:intergenic 70.0%
! ACGAGGGTACGGGGCGGACA+CGG - chr3.1:61881641-61881660 MS.gene052613:intron 70.0%
GTGTCCGCCCCGTACCCTCG+TGG + chr3.1:61881642-61881661 None:intergenic 75.0%
TACCCGCCCCGTGCCCGTGA+TGG + chr3.1:61881154-61881173 None:intergenic 75.0%
! CGAGGGTACGGGGCGGACAC+GGG - chr3.1:61881642-61881661 MS.gene052613:intron 75.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 61880939 61882064 61880939 ID=MS.gene052613
chr3.1 mRNA 61880939 61882064 61880939 ID=MS.gene052613.t1;Parent=MS.gene052613
chr3.1 exon 61881961 61882064 61881961 ID=MS.gene052613.t1.exon1;Parent=MS.gene052613.t1
chr3.1 CDS 61881961 61882064 61881961 ID=cds.MS.gene052613.t1;Parent=MS.gene052613.t1
chr3.1 exon 61880939 61881018 61880939 ID=MS.gene052613.t1.exon2;Parent=MS.gene052613.t1
chr3.1 CDS 61880939 61881018 61880939 ID=cds.MS.gene052613.t1;Parent=MS.gene052613.t1
Gene Sequence

>MS.gene052613

GTACCGCGGAAAACGGATGAGAATAGACTATTTCATAGTTTCAGAAAAACTTAAAGAAAAGATTGTTGCATGTGAAATGCATGGAAAAGGGATAGAGTTACAAGGTAAGTTTCTTTTCGTGTGATGATACTGGTTGCTTACTCAACAAAATTTGATATTCGTTTTTAGGGGTGGCAAAAATACCCATGCCCACGGGTATCCGTGGGTAAAATCCATCACGGGCACGGGGCGGGTAGCTAAACGGGTATCCACGGGTATAATAAATGGGTATTTGACTACCCATTATTATATGGATACGGACACGGATTTGATTGTACTCATACCCACGGGTACTCATACCCGATAATAAATTAATTAAATTACTAAAATATCCTTAGACATCTTATTACTTAAATTGTTACCTTATTGAAGCAAATTTTACCGCCCACCTAAGATTCTTCATTGATAACCTATTTTTATTTAGAATTTTTTTAATTCATCTTATTAAGTTCACCGTTTCCACTTCATTCACATCATAAAACATATCCTATGGATTATGTATGATTTATGAAATTTTGAATGGATGATGTATGGATGTTTAAAAGTTGAAGTTTTTTGGTAATTAAAAAAGTTATTATTTGTACCAAAAAAATAAATAAATTAAAAAATTCATGGGTACCCTGGGATACCTCAATACCCGCGGGTACCCGTATAGTGGATACCACGAGGGTACGGGGCGGACACGGGTATCATATTTATCCAACGGGGCGGGGACTGGTATCATGCTATCCGTACCCATGGGTACCCATTGACATCCCTCCTTTTATGTATATGGTTCCTCCTTTAGCTTATCTAAGTTAAAAAACTGAATCCAGAGTGCAACTTTGTGGTAATTGGTGTTGTTGGGTTGTATCTAACCTGTTTAATGGCTACCCCAAAAATTAGGCTTTGCTGTCATTTTCATATGCATGGGTTTCTTGTGCATATCTCTCCATTGTTATTATCATTTTCAATAACTTTTCATTAGCTTATGTTTCAGAAGTATGACCTTGTTGTTGTCTCCTCAGGTTTCTATGGAAGTGATCATTGTCCTGTTACCTTGGAGCTCTCTCCTAGTTCCAACTCTCAAAATGAGGATCCAGTATAA

Protein sequence

>MS.gene052613.t1

VPRKTDENRLFHSFRKTURKDCCMUNAWKRDRVTRFLWKUSLSCYLGALSUFQLSKUGSSI