AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44464

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44464.t1 MTR_4g097080 96.667 510 11 3 1 510 1 504 0.0 1021
MS.gene44464.t1 MTR_5g015490 67.816 522 125 14 1 510 1 491 0.0 643
MS.gene44464.t1 MTR_2g099110 52.758 417 170 9 107 510 50 452 2.97e-143 419
MS.gene44464.t1 MTR_2g082090 31.937 382 239 6 135 508 210 578 1.54e-59 206
MS.gene44464.t1 MTR_2g089100 29.004 462 275 9 81 510 4 444 1.68e-59 203
MS.gene44464.t1 MTR_5g094450 30.366 382 251 5 135 509 159 532 5.10e-59 204
MS.gene44464.t1 MTR_5g097480 30.469 384 244 7 135 509 175 544 8.76e-59 203
MS.gene44464.t1 MTR_1g029420 31.088 386 239 8 135 509 223 592 4.40e-58 202
MS.gene44464.t1 MTR_3g056110 29.397 398 250 9 125 509 163 542 1.36e-54 192
MS.gene44464.t1 MTR_4g095500 28.243 478 285 11 80 510 3 469 7.84e-54 188
MS.gene44464.t1 MTR_3g089055 29.737 380 252 6 135 509 196 565 2.04e-53 189
MS.gene44464.t1 MTR_3g089055 29.737 380 252 6 135 509 201 570 2.05e-53 189
MS.gene44464.t1 MTR_5g015950 27.842 431 258 8 126 510 24 447 2.28e-51 181
MS.gene44464.t1 MTR_3g053270 27.842 431 258 8 126 510 24 447 2.28e-51 181
MS.gene44464.t1 MTR_7g027190 29.873 395 236 10 136 509 299 673 6.16e-51 184
MS.gene44464.t1 MTR_4g133660 30.570 386 240 10 135 508 173 542 4.49e-50 180
MS.gene44464.t1 MTR_8g020840 32.494 397 233 13 133 509 167 548 2.66e-49 178
MS.gene44464.t1 MTR_2g026250 29.494 356 236 6 159 509 251 596 8.52e-49 177
MS.gene44464.t1 MTR_4g102790 26.478 389 256 8 136 510 291 663 1.15e-42 161
MS.gene44464.t1 MTR_7g074650 28.191 376 242 11 140 510 441 793 1.24e-42 162
MS.gene44464.t1 MTR_4g077760 29.443 377 246 8 138 509 194 555 2.02e-42 159
MS.gene44464.t1 MTR_3g065980 26.943 386 250 7 138 509 173 540 5.82e-41 155
MS.gene44464.t1 MTR_2g097310 26.842 380 260 9 137 510 272 639 9.80e-40 152
MS.gene44464.t1 MTR_2g097410 29.659 381 252 8 137 510 371 742 1.50e-39 153
MS.gene44464.t1 MTR_4g064200 28.084 381 254 10 137 510 284 651 3.37e-39 151
MS.gene44464.t1 MTR_4g064160 27.368 380 260 7 137 510 316 685 4.67e-39 151
MS.gene44464.t1 MTR_8g442410 28.796 382 250 12 138 509 156 525 4.72e-39 149
MS.gene44464.t1 MTR_4g064150 27.273 385 262 7 135 510 359 734 8.60e-39 150
MS.gene44464.t1 MTR_2g097350 26.247 381 261 9 137 510 274 641 1.39e-38 149
MS.gene44464.t1 MTR_4g064120 27.937 383 253 10 135 506 252 622 2.45e-38 148
MS.gene44464.t1 MTR_7g062120 24.672 381 273 5 135 510 370 741 8.63e-38 148
MS.gene44464.t1 MTR_4g064180 27.937 383 253 10 135 506 252 622 9.53e-37 144
MS.gene44464.t1 MTR_2g097390 25.587 383 261 9 137 510 275 642 1.64e-34 137
MS.gene44464.t1 MTR_4g104020 25.575 391 254 10 137 509 149 520 2.32e-34 135
MS.gene44464.t1 MTR_3g022830 23.888 427 254 10 138 509 26 436 5.86e-34 133
MS.gene44464.t1 MTR_4g076020 26.842 380 233 14 140 510 87 430 2.08e-33 132
MS.gene44464.t1 MTR_2g097467 24.409 381 267 8 137 510 289 655 6.37e-33 133
MS.gene44464.t1 MTR_1g069725 27.441 379 243 12 137 508 109 462 2.54e-32 129
MS.gene44464.t1 MTR_2g097463 25.330 379 266 8 137 510 290 656 6.49e-31 127
MS.gene44464.t1 MTR_2g097473 24.538 379 269 7 137 510 289 655 1.20e-30 126
MS.gene44464.t1 MTR_3g072710 26.478 389 262 11 136 508 119 499 1.22e-28 119
MS.gene44464.t1 MTR_7g057230 25.995 427 267 11 103 508 258 656 5.01e-28 118
MS.gene44464.t1 MTR_1g096030 25.686 401 251 13 136 509 79 459 1.09e-27 115
MS.gene44464.t1 MTR_2g034260 23.940 401 284 7 124 509 190 584 2.08e-27 116
MS.gene44464.t1 MTR_2g097380 26.053 380 228 13 140 510 227 562 4.54e-27 115
MS.gene44464.t1 MTR_5g058860 26.992 389 258 12 136 508 118 496 1.65e-26 113
MS.gene44464.t1 MTR_6g047750 25.505 396 250 12 134 509 292 662 2.91e-26 113
MS.gene44464.t1 MTR_2g034250 23.632 402 285 8 124 509 190 585 8.07e-26 111
MS.gene44464.t1 MTR_5g009080 30.612 196 112 3 160 347 79 258 7.55e-25 107
MS.gene44464.t1 MTR_5g009080 30.323 155 99 4 357 509 331 478 1.66e-12 70.1
MS.gene44464.t1 MTR_4g076140 31.308 214 135 3 136 347 47 250 2.73e-24 106
MS.gene44464.t1 MTR_2g034280 24.752 404 275 11 124 509 183 575 4.49e-24 106
MS.gene44464.t1 MTR_1g086970 25.000 360 249 9 159 509 131 478 7.74e-24 104
MS.gene44464.t1 MTR_8g093070 26.615 387 245 14 136 510 138 497 4.07e-23 102
MS.gene44464.t1 MTR_7g109580 22.616 451 325 9 67 509 120 554 6.43e-23 102
MS.gene44464.t1 MTR_4g026485 22.691 379 262 13 138 509 270 624 5.14e-20 94.0
MS.gene44464.t1 MTR_8g077940 23.529 459 313 14 63 509 109 541 2.90e-17 85.1
MS.gene44464.t1 MTR_0092s0100 21.883 393 274 12 127 509 362 731 5.11e-17 84.7
MS.gene44464.t1 MTR_0092s0100 21.883 393 274 12 127 509 359 728 5.92e-17 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44464.t1 AT4G37650 57.353 544 184 13 1 510 1 530 0.0 549
MS.gene44464.t1 AT2G04890 31.746 378 236 7 137 509 53 413 1.44e-61 207
MS.gene44464.t1 AT1G50600 29.439 428 276 8 91 509 116 526 2.14e-58 202
MS.gene44464.t1 AT1G50600 29.673 428 275 9 91 509 187 597 6.06e-58 202
MS.gene44464.t1 AT1G50600 29.439 428 276 8 91 509 191 601 6.98e-58 202
MS.gene44464.t1 AT3G49950 32.308 390 242 7 135 510 28 409 5.66e-56 192
MS.gene44464.t1 AT5G48150 28.382 377 256 5 138 509 123 490 2.07e-54 191
MS.gene44464.t1 AT5G48150 28.382 377 256 5 138 509 123 490 2.07e-54 191
MS.gene44464.t1 AT5G48150 28.382 377 256 5 138 509 123 490 2.07e-54 191
MS.gene44464.t1 AT5G48150 28.382 377 256 5 138 509 123 490 2.07e-54 191
MS.gene44464.t1 AT1G21450 31.328 399 238 9 127 509 215 593 6.44e-54 191
MS.gene44464.t1 AT1G66350 31.117 376 230 10 138 509 156 506 2.47e-48 174
MS.gene44464.t1 AT3G03450 28.534 382 247 7 136 509 182 545 3.02e-47 172
MS.gene44464.t1 AT4G17230 27.344 384 258 6 135 510 156 526 9.18e-46 168
MS.gene44464.t1 AT4G17230 27.344 384 258 6 135 510 156 526 9.18e-46 168
MS.gene44464.t1 AT5G17490 29.101 378 240 7 138 509 161 516 6.84e-45 165
MS.gene44464.t1 AT3G54220 29.024 379 238 12 140 510 296 651 1.13e-42 161
MS.gene44464.t1 AT1G14920 28.533 375 247 8 138 509 173 529 3.10e-42 158
MS.gene44464.t1 AT2G01570 27.467 375 251 8 138 509 225 581 7.99e-42 158
MS.gene44464.t1 AT2G29060 28.640 419 268 12 109 510 252 656 1.44e-41 158
MS.gene44464.t1 AT2G29060 28.640 419 268 12 109 510 289 693 2.03e-41 158
MS.gene44464.t1 AT3G13840 30.928 388 236 13 133 509 144 510 4.35e-41 154
MS.gene44464.t1 AT5G66770 29.189 370 245 8 145 509 227 584 3.56e-40 153
MS.gene44464.t1 AT1G07530 26.632 383 264 8 135 510 394 766 1.05e-38 150
MS.gene44464.t1 AT2G29065 26.425 386 268 9 135 510 250 629 2.09e-38 149
MS.gene44464.t1 AT2G37650 26.247 381 265 7 135 510 333 702 2.27e-38 149
MS.gene44464.t1 AT2G37650 26.247 381 265 7 135 510 345 714 2.56e-38 149
MS.gene44464.t1 AT1G50420 25.792 442 242 8 136 509 56 479 2.18e-37 144
MS.gene44464.t1 AT1G63100 27.506 389 255 9 136 508 276 653 4.14e-36 142
MS.gene44464.t1 AT3G46600 26.963 382 262 7 135 510 2 372 4.49e-36 138
MS.gene44464.t1 AT1G63100 27.506 389 255 9 136 508 251 628 4.82e-36 142
MS.gene44464.t1 AT3G46600 26.963 382 262 7 135 510 210 580 2.68e-35 139
MS.gene44464.t1 AT3G46600 26.963 382 262 7 135 510 178 548 4.91e-35 138
MS.gene44464.t1 AT5G59450 24.852 507 338 11 21 510 119 599 3.17e-34 136
MS.gene44464.t1 AT3G50650 29.248 359 231 9 160 509 198 542 2.74e-33 133
MS.gene44464.t1 AT5G41920 27.676 383 244 13 136 509 42 400 2.24e-32 128
MS.gene44464.t1 AT5G52510 24.874 398 255 9 134 509 265 640 3.62e-32 130
MS.gene44464.t1 AT1G07520 26.684 386 266 9 135 510 316 694 4.74e-32 130
MS.gene44464.t1 AT1G07520 26.684 386 266 9 135 510 317 695 5.27e-32 130
MS.gene44464.t1 AT1G55580 29.017 417 237 14 137 509 44 445 5.74e-32 128
MS.gene44464.t1 AT1G07520 26.684 386 266 9 135 510 152 530 8.18e-32 129
MS.gene44464.t1 AT1G07520 26.684 386 266 9 135 510 152 530 8.18e-32 129
MS.gene44464.t1 AT4G08250 28.859 298 198 8 217 508 187 476 4.19e-26 111
MS.gene44464.t1 AT3G60630 25.497 302 204 9 217 509 333 622 5.48e-15 78.2
MS.gene44464.t1 AT4G00150 24.282 383 241 15 139 509 209 554 1.28e-14 77.0
MS.gene44464.t1 AT4G36710 22.038 422 286 14 96 509 99 485 5.40e-12 68.6
MS.gene44464.t1 AT2G45160 23.362 351 230 12 172 509 315 639 1.61e-11 67.4

Find 122 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATCAATTATGTGAAGTTT+AGG 0.248165 8.1:+25376900 None:intergenic
AAACAAATCACGGTGGTTTC+CGG 0.257711 8.1:+25376578 None:intergenic
AAGAGTGTTTAAGATGGTTT+AGG 0.258495 8.1:-25376455 MS.gene44464:CDS
TATCAATTATGTGAAGTTTA+GGG 0.283325 8.1:+25376901 None:intergenic
ATTCTTCGTCTTCTTCTTGT+TGG 0.301725 8.1:+25377483 None:intergenic
AGGAGGAGGTTGCATGGTGG+TGG 0.328666 8.1:-25376285 MS.gene44464:CDS
TTGTCCATGGACTAACTTCT+TGG 0.329314 8.1:+25376976 None:intergenic
TAAGTAGTGTTTGTTGTGTT+TGG 0.339821 8.1:+25377341 None:intergenic
ATGAAGTTTGTGATGATGTT+AGG 0.340214 8.1:-25376239 MS.gene44464:CDS
GCTGATTTAGAAGTTTGTTT+TGG 0.347659 8.1:-25376501 MS.gene44464:CDS
AGAGTGTTTAAGATGGTTTA+GGG 0.373474 8.1:-25376454 MS.gene44464:CDS
TGGAAGAGGTATTGTTGATT+TGG 0.374691 8.1:-25376361 MS.gene44464:CDS
ATCACGGTGGTTTCCGGCTC+CGG 0.383080 8.1:+25376584 None:intergenic
GTTAAATGGATACGTTGTTT+AGG 0.388354 8.1:-25377631 None:intergenic
AACTCTTTACATTCGATATC+CGG 0.397888 8.1:-25376603 MS.gene44464:CDS
TCCACATGAGTTGTTGGATT+CGG 0.400997 8.1:+25377174 None:intergenic
TACTTCAACTTTGAATTCTC+CGG 0.412778 8.1:-25377260 MS.gene44464:CDS
CATTAAAGAAGATGAAGCTT+TGG 0.420300 8.1:-25376640 MS.gene44464:CDS
GTTAGTCCATGGACAACGTT+TGG 0.420735 8.1:-25376969 MS.gene44464:CDS
AAAATGCACGTGCGGTTTCA+AGG 0.430548 8.1:+25377213 None:intergenic
CGGTGGTGTGGGCCAGTGTA+TGG 0.430711 8.1:-25376125 MS.gene44464:CDS
CTTCCACGACAAGAATATTC+CGG 0.431546 8.1:+25376155 None:intergenic
GCATGGTGGTGGGTTTAATA+CGG 0.431980 8.1:-25376274 MS.gene44464:CDS
GGAGGAGGTTGCATGGTGGT+GGG 0.435450 8.1:-25376284 MS.gene44464:CDS
TGTTTATATCTTTGCTTCGT+GGG 0.441689 8.1:-25376560 MS.gene44464:CDS
TTAGCATCCACATGAGTTGT+TGG 0.445709 8.1:+25377168 None:intergenic
TTGTTTATATCTTTGCTTCG+TGG 0.451946 8.1:-25376561 MS.gene44464:CDS
TGCTTCGTGGGTTAGAACCT+AGG 0.461690 8.1:-25376548 MS.gene44464:CDS
CGTCTCTCCATTGACTCATA+AGG 0.462546 8.1:+25376327 None:intergenic
CAGCGTCACGGTGGAGGAGG+AGG 0.463327 8.1:-25376299 MS.gene44464:CDS
TTTGGTTCGGATTTCGTGGA+AGG 0.470121 8.1:-25376483 MS.gene44464:CDS
CACCGCAGTCAGCGGTGGCT+CGG 0.470184 8.1:-25376784 MS.gene44464:CDS
GATTCGGTTTGTGTTGTTGT+CGG 0.471253 8.1:+25377190 None:intergenic
GCTATCTTAGAAGCATTAGA+AGG 0.474912 8.1:-25376927 MS.gene44464:CDS
TAAGGTGGTGGTGGAGTTGA+AGG 0.476215 8.1:+25377559 None:intergenic
ATGTTAGGGCTTTGTTGAGA+AGG 0.482294 8.1:-25376224 MS.gene44464:CDS
CGGTGACTACAGTGGTTAAA+CGG 0.484162 8.1:+25376802 None:intergenic
GTGGAGTTGAAGGAAGGATC+AGG 0.484573 8.1:+25377569 None:intergenic
TGAAGTTTGTGATGATGTTA+GGG 0.489537 8.1:-25376238 MS.gene44464:CDS
AGAGCTGCTAGAAGTTGTTA+AGG 0.490994 8.1:+25377541 None:intergenic
TCATGAAGGAAATCGGTTCA+AGG 0.493998 8.1:-25376752 MS.gene44464:CDS
GCAACACTTATTGCACTCAA+TGG 0.496242 8.1:-25376875 MS.gene44464:CDS
TTGAATTCTCCGGCAACTCA+TGG 0.501115 8.1:-25377250 MS.gene44464:CDS
GCTTCGTGGGTTAGAACCTA+GGG 0.504275 8.1:-25376547 MS.gene44464:CDS
GTAATTATCAGTTGTGGTGG+GGG 0.506791 8.1:+25377304 None:intergenic
TTGATGCTAGAAAGAGAAGC+TGG 0.507592 8.1:-25376381 MS.gene44464:CDS
AGCGGGTGGCCAACGCTTCG+AGG 0.508942 8.1:+25376844 None:intergenic
ATAGTAGTAGTGGATGCATA+AGG 0.510603 8.1:+25377371 None:intergenic
AGAGTAATTATCAGTTGTGG+TGG 0.511198 8.1:+25377301 None:intergenic
TGTTCCTCCGATGGTGATGC+CGG 0.512206 8.1:-25376174 MS.gene44464:CDS
GAGTAATTATCAGTTGTGGT+GGG 0.513221 8.1:+25377302 None:intergenic
TTTGATCTGTGTCACCGTAT+GGG 0.514015 8.1:+25377132 None:intergenic
CGGTGGAGGAGGAGGTTGCA+TGG 0.514673 8.1:-25376291 MS.gene44464:CDS
GAAGGATAAGCCGGTGGTGT+GGG 0.516813 8.1:-25376136 MS.gene44464:CDS
GGAAGGATAAGCCGGTGGTG+TGG 0.518070 8.1:-25376137 MS.gene44464:CDS
CGGAATATTCTTGTCGTGGA+AGG 0.518332 8.1:-25376154 MS.gene44464:CDS
CTAGAAAGAGAAGCTGGAAG+AGG 0.518793 8.1:-25376375 MS.gene44464:CDS
AAACAGCGTCACGGTGGAGG+AGG 0.519490 8.1:-25376302 MS.gene44464:CDS
CATACACTGGCCCACACCAC+CGG 0.526670 8.1:+25376126 None:intergenic
TTGATCTGTGTCACCGTATG+GGG 0.527505 8.1:+25377133 None:intergenic
TTGTTGAGAAGGTATAAAGA+AGG 0.530989 8.1:-25376213 MS.gene44464:CDS
AGAAGTTGTTAAGGTGGTGG+TGG 0.532155 8.1:+25377550 None:intergenic
ACCTCAAGGCCTCCATACAC+TGG 0.532917 8.1:+25376113 None:intergenic
TGCTTCTAAGATAGCACCGT+TGG 0.532927 8.1:+25376935 None:intergenic
GCCAACACTCCTCGAAGCGT+TGG 0.539050 8.1:-25376853 MS.gene44464:CDS
ACGGAGGTGAGGCGTATCAT+CGG 0.541385 8.1:+25376821 None:intergenic
TGTTGAGAAGGTATAAAGAA+GGG 0.543842 8.1:-25376212 MS.gene44464:CDS
TCAACAACAGTTAAAACCCT+AGG 0.548008 8.1:+25376531 None:intergenic
CATCTTCCTCGTTGAATCGA+AGG 0.549319 8.1:+25377010 None:intergenic
GTTTAGTCGCATGAACGACA+CGG 0.549663 8.1:-25377081 MS.gene44464:CDS
ACGTGTCCAAACGTTGTCCA+TGG 0.550032 8.1:+25376963 None:intergenic
AGTAATTATCAGTTGTGGTG+GGG 0.552091 8.1:+25377303 None:intergenic
GAAGGAAATCGGTTCAAGGA+TGG 0.552770 8.1:-25376748 MS.gene44464:CDS
GCCAACGCTTCGAGGAGTGT+TGG 0.553618 8.1:+25376852 None:intergenic
TTTAGTCGCATGAACGACAC+GGG 0.557326 8.1:-25377080 MS.gene44464:CDS
TTGTGATCCTTATGAGTCAA+TGG 0.560449 8.1:-25376334 MS.gene44464:CDS
ATTCAAAGTTGAAGTAATCG+TGG 0.563080 8.1:+25377267 None:intergenic
TTGATATGTTGTTTGTCTTG+AGG 0.563090 8.1:+25377514 None:intergenic
GTGAGGCGTATCATCGGAGC+GGG 0.565125 8.1:+25376827 None:intergenic
GTGGTGGTGGAGTTGAAGGA+AGG 0.565220 8.1:+25377563 None:intergenic
TGGAGTAGTGATAGTAGTAG+TGG 0.565967 8.1:+25377361 None:intergenic
GCCAGTGTATGGAGGCCTTG+AGG 0.566308 8.1:-25376114 None:intergenic
GTGAAACAGCGTCACGGTGG+AGG 0.572256 8.1:-25376305 MS.gene44464:CDS
GAATATTCCGGCATCACCAT+CGG 0.580354 8.1:+25376167 None:intergenic
TTTCAAGAACAAGTAGTGAG+AGG 0.582987 8.1:-25376404 MS.gene44464:CDS
AGTTCCAAGAAGTTAGTCCA+TGG 0.584447 8.1:-25376980 MS.gene44464:CDS
TTACATTCGATATCCGGAGC+CGG 0.585693 8.1:-25376597 MS.gene44464:CDS
ATGGACAACGTTTGGACACG+TGG 0.586613 8.1:-25376961 MS.gene44464:CDS
TTCAAAGTTGAAGTAATCGT+GGG 0.586851 8.1:+25377268 None:intergenic
ATGACACAGTGTTCCTCCGA+TGG 0.587322 8.1:-25376183 MS.gene44464:CDS
TTTGGACACGTGGCAGCCAA+CGG 0.587329 8.1:-25376951 MS.gene44464:CDS
CTTGTCGTGGAAGGATAAGC+CGG 0.587868 8.1:-25376145 MS.gene44464:CDS
GTGTTGTTTAGAAGAAGATG+AGG 0.594437 8.1:+25377406 None:intergenic
GAAAGAGTAATTATCAGTTG+TGG 0.594879 8.1:+25377298 None:intergenic
GCAAAGATATAAACAAATCA+CGG 0.596997 8.1:+25376568 None:intergenic
GTAGTCACCGCAGTCAGCGG+TGG 0.597985 8.1:-25376789 MS.gene44464:CDS
CACCGAGCCACCGCTGACTG+CGG 0.600692 8.1:+25376782 None:intergenic
AGGCGTATCATCGGAGCGGG+TGG 0.604096 8.1:+25376830 None:intergenic
TGGTGTGGGCCAGTGTATGG+AGG 0.609272 8.1:-25376122 MS.gene44464:CDS
ATGCCGGAATATTCTTGTCG+TGG 0.614564 8.1:-25376158 MS.gene44464:CDS
ACCGAATCCAACAACTCATG+TGG 0.616002 8.1:-25377175 MS.gene44464:CDS
AACGAGCTTAGTTCCCCATA+CGG 0.616114 8.1:-25377146 MS.gene44464:CDS
GCTAGAAGTTGTTAAGGTGG+TGG 0.616229 8.1:+25377547 None:intergenic
AAGTTGAAGTAATCGTGGGA+GGG 0.628358 8.1:+25377272 None:intergenic
GCTGCTAGAAGTTGTTAAGG+TGG 0.637704 8.1:+25377544 None:intergenic
AAAGTTGAAGTAATCGTGGG+AGG 0.640378 8.1:+25377271 None:intergenic
TGAGAAGGTATAAAGAAGGG+TGG 0.652792 8.1:-25376209 MS.gene44464:CDS
ACTGTAGTCACCGCAGTCAG+CGG 0.657652 8.1:-25376792 MS.gene44464:CDS
ACAGTGGTTAAACGGAGGTG+AGG 0.666216 8.1:+25376810 None:intergenic
CAAAACCTTAACATCCGCGT+CGG 0.669594 8.1:-25377045 MS.gene44464:CDS
GTCGTGGAAGGATAAGCCGG+TGG 0.674105 8.1:-25376142 MS.gene44464:CDS
TATTCCGGCATCACCATCGG+AGG 0.676733 8.1:+25376170 None:intergenic
CATGTTCCTTCGATTCAACG+AGG 0.690083 8.1:-25377016 MS.gene44464:CDS
GGTGAGGCGTATCATCGGAG+CGG 0.690322 8.1:+25376826 None:intergenic
TGGAGGAGGAGGTTGCATGG+TGG 0.690855 8.1:-25376288 MS.gene44464:CDS
GTTGTCGGAAAATGCACGTG+CGG 0.703618 8.1:+25377205 None:intergenic
GCTGACTGCGGTGACTACAG+TGG 0.705744 8.1:+25376794 None:intergenic
AGAGACGTGAAACAGCGTCA+CGG 0.722636 8.1:-25376311 MS.gene44464:CDS
GACGTGAAACAGCGTCACGG+TGG 0.727045 8.1:-25376308 MS.gene44464:CDS
ATGCACGTGCGGTTTCAAGG+AGG 0.744530 8.1:+25377216 None:intergenic
TGACTACAGTGGTTAAACGG+AGG 0.748940 8.1:+25376805 None:intergenic
AAGATATAAACAAATCACGG+TGG 0.755243 8.1:+25376571 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATGATTTTGAATTTAAA+CGG + chr8.1:25377046-25377065 None:intergenic 10.0%
!! ATATCAATTATGTGAAGTTT+AGG + chr8.1:25376845-25376864 None:intergenic 20.0%
!! TATCAATTATGTGAAGTTTA+GGG + chr8.1:25376844-25376863 None:intergenic 20.0%
! GCAAAGATATAAACAAATCA+CGG + chr8.1:25377177-25377196 None:intergenic 25.0%
!! CTTCAACAACTTTTATTACA+TGG - chr8.1:25376283-25376302 MS.gene44464:CDS 25.0%
!!! GTTTTAAAGAGTGTTTAAGA+TGG - chr8.1:25377281-25377300 MS.gene44464:CDS 25.0%
!!! TTTAGAAGTTTGTTTTGGTT+CGG - chr8.1:25377246-25377265 MS.gene44464:CDS 25.0%
AACTCTTTACATTCGATATC+CGG - chr8.1:25377139-25377158 MS.gene44464:CDS 30.0%
AAGATATAAACAAATCACGG+TGG + chr8.1:25377174-25377193 None:intergenic 30.0%
ATTCAAAGTTGAAGTAATCG+TGG + chr8.1:25376478-25376497 None:intergenic 30.0%
CAAAAAGTCATGAAGGAAAT+CGG - chr8.1:25376983-25377002 MS.gene44464:CDS 30.0%
CATTAAAGAAGATGAAGCTT+TGG - chr8.1:25377102-25377121 MS.gene44464:CDS 30.0%
GAAAGAGTAATTATCAGTTG+TGG + chr8.1:25376447-25376466 None:intergenic 30.0%
TACTTCAACTTTGAATTCTC+CGG - chr8.1:25376482-25376501 MS.gene44464:CDS 30.0%
TGTTTATATCTTTGCTTCGT+GGG - chr8.1:25377182-25377201 MS.gene44464:CDS 30.0%
TTCAAAGTTGAAGTAATCGT+GGG + chr8.1:25376477-25376496 None:intergenic 30.0%
TTGTTTATATCTTTGCTTCG+TGG - chr8.1:25377181-25377200 MS.gene44464:CDS 30.0%
! AGAGTGTTTAAGATGGTTTA+GGG - chr8.1:25377288-25377307 MS.gene44464:CDS 30.0%
! ATGAAGTTTGTGATGATGTT+AGG - chr8.1:25377503-25377522 MS.gene44464:CDS 30.0%
! TGAAGTTTGTGATGATGTTA+GGG - chr8.1:25377504-25377523 MS.gene44464:CDS 30.0%
! TGTTGAGAAGGTATAAAGAA+GGG - chr8.1:25377530-25377549 MS.gene44464:CDS 30.0%
! TTGATATGTTGTTTGTCTTG+AGG + chr8.1:25376231-25376250 None:intergenic 30.0%
! TTGTTGAGAAGGTATAAAGA+AGG - chr8.1:25377529-25377548 MS.gene44464:CDS 30.0%
!! AAGAGTGTTTAAGATGGTTT+AGG - chr8.1:25377287-25377306 MS.gene44464:CDS 30.0%
!! GCTGATTTAGAAGTTTGTTT+TGG - chr8.1:25377241-25377260 MS.gene44464:CDS 30.0%
!! TAAGTAGTGTTTGTTGTGTT+TGG + chr8.1:25376404-25376423 None:intergenic 30.0%
AGAGTAATTATCAGTTGTGG+TGG + chr8.1:25376444-25376463 None:intergenic 35.0%
AGTAATTATCAGTTGTGGTG+GGG + chr8.1:25376442-25376461 None:intergenic 35.0%
ATTCTTCGTCTTCTTCTTGT+TGG + chr8.1:25376262-25376281 None:intergenic 35.0%
GAGTAATTATCAGTTGTGGT+GGG + chr8.1:25376443-25376462 None:intergenic 35.0%
TCAACAACAGTTAAAACCCT+AGG + chr8.1:25377214-25377233 None:intergenic 35.0%
TTGTGATCCTTATGAGTCAA+TGG - chr8.1:25377408-25377427 MS.gene44464:CDS 35.0%
TTTCAAGAACAAGTAGTGAG+AGG - chr8.1:25377338-25377357 MS.gene44464:CDS 35.0%
! CAAGGATGGAAAAATTTGCT+AGG - chr8.1:25377008-25377027 MS.gene44464:CDS 35.0%
!! ATAGTAGTAGTGGATGCATA+AGG + chr8.1:25376374-25376393 None:intergenic 35.0%
!! GAAAAATTTGCTAGGCTCAT+GGG - chr8.1:25377016-25377035 MS.gene44464:CDS 35.0%
!! GCTATCTTAGAAGCATTAGA+AGG - chr8.1:25376815-25376834 MS.gene44464:CDS 35.0%
!! GTGTTGTTTAGAAGAAGATG+AGG + chr8.1:25376339-25376358 None:intergenic 35.0%
!! TGGAAGAGGTATTGTTGATT+TGG - chr8.1:25377381-25377400 MS.gene44464:CDS 35.0%
!!! GGTTTTAACTGTTGTTGAAG+AGG - chr8.1:25377216-25377235 MS.gene44464:CDS 35.0%
AAACAAATCACGGTGGTTTC+CGG + chr8.1:25377167-25377186 None:intergenic 40.0%
AAAGTTGAAGTAATCGTGGG+AGG + chr8.1:25376474-25376493 None:intergenic 40.0%
AAGTTGAAGTAATCGTGGGA+GGG + chr8.1:25376473-25376492 None:intergenic 40.0%
AGAGCTGCTAGAAGTTGTTA+AGG + chr8.1:25376204-25376223 None:intergenic 40.0%
AGTTCCAAGAAGTTAGTCCA+TGG - chr8.1:25376762-25376781 MS.gene44464:CDS 40.0%
CTTCCACGACAAGAATATTC+CGG + chr8.1:25377590-25377609 None:intergenic 40.0%
GCAACACTTATTGCACTCAA+TGG - chr8.1:25376867-25376886 MS.gene44464:CDS 40.0%
GTAATTATCAGTTGTGGTGG+GGG + chr8.1:25376441-25376460 None:intergenic 40.0%
TCCACATGAGTTGTTGGATT+CGG + chr8.1:25376571-25376590 None:intergenic 40.0%
TGGAGTAGTGATAGTAGTAG+TGG + chr8.1:25376384-25376403 None:intergenic 40.0%
TTAGCATCCACATGAGTTGT+TGG + chr8.1:25376577-25376596 None:intergenic 40.0%
TTGATGCTAGAAAGAGAAGC+TGG - chr8.1:25377361-25377380 MS.gene44464:CDS 40.0%
TTTGATCTGTGTCACCGTAT+GGG + chr8.1:25376613-25376632 None:intergenic 40.0%
! GATTCGGTTTGTGTTGTTGT+CGG + chr8.1:25376555-25376574 None:intergenic 40.0%
! TCATGAAGGAAATCGGTTCA+AGG - chr8.1:25376990-25377009 MS.gene44464:CDS 40.0%
! TGAGAAGGTATAAAGAAGGG+TGG - chr8.1:25377533-25377552 MS.gene44464:CDS 40.0%
! TTGTCCATGGACTAACTTCT+TGG + chr8.1:25376769-25376788 None:intergenic 40.0%
! TTTTGATCTGTGTCACCGTA+TGG + chr8.1:25376614-25376633 None:intergenic 40.0%
!! ATGTTAGGGCTTTGTTGAGA+AGG - chr8.1:25377518-25377537 MS.gene44464:CDS 40.0%
!! GGAAAAATTTGCTAGGCTCA+TGG - chr8.1:25377015-25377034 MS.gene44464:CDS 40.0%
!!! TTGTTTTGGTTCGGATTTCG+TGG - chr8.1:25377255-25377274 MS.gene44464:CDS 40.0%
AAAATGCACGTGCGGTTTCA+AGG + chr8.1:25376532-25376551 None:intergenic 45.0%
AACGAGCTTAGTTCCCCATA+CGG - chr8.1:25376596-25376615 MS.gene44464:CDS 45.0%
ACCGAATCCAACAACTCATG+TGG - chr8.1:25376567-25376586 MS.gene44464:CDS 45.0%
ATGCCGGAATATTCTTGTCG+TGG - chr8.1:25377584-25377603 MS.gene44464:CDS 45.0%
CAAAACCTTAACATCCGCGT+CGG - chr8.1:25376697-25376716 MS.gene44464:CDS 45.0%
CATCTTCCTCGTTGAATCGA+AGG + chr8.1:25376735-25376754 None:intergenic 45.0%
CATGTTCCTTCGATTCAACG+AGG - chr8.1:25376726-25376745 MS.gene44464:CDS 45.0%
CGGAATATTCTTGTCGTGGA+AGG - chr8.1:25377588-25377607 MS.gene44464:CDS 45.0%
CGGTGACTACAGTGGTTAAA+CGG + chr8.1:25376943-25376962 None:intergenic 45.0%
CTAGAAAGAGAAGCTGGAAG+AGG - chr8.1:25377367-25377386 MS.gene44464:CDS 45.0%
GAATATTCCGGCATCACCAT+CGG + chr8.1:25377578-25377597 None:intergenic 45.0%
GCTAGAAGTTGTTAAGGTGG+TGG + chr8.1:25376198-25376217 None:intergenic 45.0%
GCTGCTAGAAGTTGTTAAGG+TGG + chr8.1:25376201-25376220 None:intergenic 45.0%
GTTAGTCCATGGACAACGTT+TGG - chr8.1:25376773-25376792 MS.gene44464:CDS 45.0%
GTTTAGTCGCATGAACGACA+CGG - chr8.1:25376661-25376680 MS.gene44464:CDS 45.0%
TGACTACAGTGGTTAAACGG+AGG + chr8.1:25376940-25376959 None:intergenic 45.0%
TTACATTCGATATCCGGAGC+CGG - chr8.1:25377145-25377164 MS.gene44464:CDS 45.0%
TTGAATTCTCCGGCAACTCA+TGG - chr8.1:25376492-25376511 MS.gene44464:CDS 45.0%
TTGATCTGTGTCACCGTATG+GGG + chr8.1:25376612-25376631 None:intergenic 45.0%
TTTAGTCGCATGAACGACAC+GGG - chr8.1:25376662-25376681 MS.gene44464:CDS 45.0%
! AGAAGTTGTTAAGGTGGTGG+TGG + chr8.1:25376195-25376214 None:intergenic 45.0%
! CTCGGTGCAAAAAGTCATGA+AGG - chr8.1:25376976-25376995 MS.gene44464:CDS 45.0%
! GAAGGAAATCGGTTCAAGGA+TGG - chr8.1:25376994-25377013 MS.gene44464:CDS 45.0%
! GTTTTGTGACCATGAGTTGC+CGG + chr8.1:25376504-25376523 None:intergenic 45.0%
! TTTTTCCGACGCGGATGTTA+AGG + chr8.1:25376705-25376724 None:intergenic 45.0%
!! CGCGGATGTTAAGGTTTTGT+AGG + chr8.1:25376696-25376715 None:intergenic 45.0%
!! CGTCTCTCCATTGACTCATA+AGG + chr8.1:25377418-25377437 None:intergenic 45.0%
!! GCATGGTGGTGGGTTTAATA+CGG - chr8.1:25377468-25377487 MS.gene44464:CDS 45.0%
!! TGCTTCTAAGATAGCACCGT+TGG + chr8.1:25376810-25376829 None:intergenic 45.0%
!! TTTGGTTCGGATTTCGTGGA+AGG - chr8.1:25377259-25377278 MS.gene44464:CDS 45.0%
!!! GGAACATGTTTTTTCCGACG+CGG + chr8.1:25376714-25376733 None:intergenic 45.0%
ACAGTGGTTAAACGGAGGTG+AGG + chr8.1:25376935-25376954 None:intergenic 50.0%
ACGTGTCCAAACGTTGTCCA+TGG + chr8.1:25376782-25376801 None:intergenic 50.0%
AGAGACGTGAAACAGCGTCA+CGG - chr8.1:25377431-25377450 MS.gene44464:CDS 50.0%
ATGACACAGTGTTCCTCCGA+TGG - chr8.1:25377559-25377578 MS.gene44464:CDS 50.0%
ATGGACAACGTTTGGACACG+TGG - chr8.1:25376781-25376800 MS.gene44464:CDS 50.0%
CTTGTCGTGGAAGGATAAGC+CGG - chr8.1:25377597-25377616 MS.gene44464:CDS 50.0%
GTGGAGTTGAAGGAAGGATC+AGG + chr8.1:25376176-25376195 None:intergenic 50.0%
GTTGTCGGAAAATGCACGTG+CGG + chr8.1:25376540-25376559 None:intergenic 50.0%
!! GCTTCGTGGGTTAGAACCTA+GGG - chr8.1:25377195-25377214 MS.gene44464:CDS 50.0%
!! TAAGGTGGTGGTGGAGTTGA+AGG + chr8.1:25376186-25376205 None:intergenic 50.0%
!! TGCTTCGTGGGTTAGAACCT+AGG - chr8.1:25377194-25377213 MS.gene44464:CDS 50.0%
ACGGAGGTGAGGCGTATCAT+CGG + chr8.1:25376924-25376943 None:intergenic 55.0%
ACTGTAGTCACCGCAGTCAG+CGG - chr8.1:25376950-25376969 MS.gene44464:CDS 55.0%
ATGCACGTGCGGTTTCAAGG+AGG + chr8.1:25376529-25376548 None:intergenic 55.0%
GAAGGATAAGCCGGTGGTGT+GGG - chr8.1:25377606-25377625 MS.gene44464:CDS 55.0%
TATTCCGGCATCACCATCGG+AGG + chr8.1:25377575-25377594 None:intergenic 55.0%
TTTGGACACGTGGCAGCCAA+CGG - chr8.1:25376791-25376810 MS.gene44464:CDS 55.0%
! TGTTCCTCCGATGGTGATGC+CGG - chr8.1:25377568-25377587 MS.gene44464:CDS 55.0%
!! GTGGTGGTGGAGTTGAAGGA+AGG + chr8.1:25376182-25376201 None:intergenic 55.0%
AAACAGCGTCACGGTGGAGG+AGG - chr8.1:25377440-25377459 MS.gene44464:CDS 60.0%
AGGAGGAGGTTGCATGGTGG+TGG - chr8.1:25377457-25377476 MS.gene44464:CDS 60.0%
ATCACGGTGGTTTCCGGCTC+CGG + chr8.1:25377161-25377180 None:intergenic 60.0%
CATACACTGGCCCACACCAC+CGG + chr8.1:25377619-25377638 None:intergenic 60.0%
GACGTGAAACAGCGTCACGG+TGG - chr8.1:25377434-25377453 MS.gene44464:CDS 60.0%
GCCAACACTCCTCGAAGCGT+TGG - chr8.1:25376889-25376908 MS.gene44464:CDS 60.0%
GCTGACTGCGGTGACTACAG+TGG + chr8.1:25376951-25376970 None:intergenic 60.0%
GGAAGGATAAGCCGGTGGTG+TGG - chr8.1:25377605-25377624 MS.gene44464:CDS 60.0%
GGAGGAGGTTGCATGGTGGT+GGG - chr8.1:25377458-25377477 MS.gene44464:CDS 60.0%
GGTGAGGCGTATCATCGGAG+CGG + chr8.1:25376919-25376938 None:intergenic 60.0%
GTCGTGGAAGGATAAGCCGG+TGG - chr8.1:25377600-25377619 MS.gene44464:CDS 60.0%
GTGAAACAGCGTCACGGTGG+AGG - chr8.1:25377437-25377456 MS.gene44464:CDS 60.0%
GTGAGGCGTATCATCGGAGC+GGG + chr8.1:25376918-25376937 None:intergenic 60.0%
TGGAGGAGGAGGTTGCATGG+TGG - chr8.1:25377454-25377473 MS.gene44464:CDS 60.0%
TGGTGTGGGCCAGTGTATGG+AGG - chr8.1:25377620-25377639 MS.gene44464:CDS 60.0%
! GCCAACGCTTCGAGGAGTGT+TGG + chr8.1:25376893-25376912 None:intergenic 60.0%
AGGCGTATCATCGGAGCGGG+TGG + chr8.1:25376915-25376934 None:intergenic 65.0%
CGGTGGAGGAGGAGGTTGCA+TGG - chr8.1:25377451-25377470 MS.gene44464:CDS 65.0%
CGGTGGTGTGGGCCAGTGTA+TGG - chr8.1:25377617-25377636 MS.gene44464:CDS 65.0%
GTAGTCACCGCAGTCAGCGG+TGG - chr8.1:25376953-25376972 MS.gene44464:CDS 65.0%
AGCGGGTGGCCAACGCTTCG+AGG + chr8.1:25376901-25376920 None:intergenic 70.0%
CACCGCAGTCAGCGGTGGCT+CGG - chr8.1:25376958-25376977 MS.gene44464:CDS 70.0%
CAGCGTCACGGTGGAGGAGG+AGG - chr8.1:25377443-25377462 MS.gene44464:CDS 70.0%
! CACCGAGCCACCGCTGACTG+CGG + chr8.1:25376963-25376982 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 25376116 25377648 25376116 ID=MS.gene44464
chr8.1 mRNA 25376116 25377648 25376116 ID=MS.gene44464.t1;Parent=MS.gene44464
chr8.1 exon 25376116 25377648 25376116 ID=MS.gene44464.t1.exon1;Parent=MS.gene44464.t1
chr8.1 CDS 25376116 25377648 25376116 ID=cds.MS.gene44464.t1;Parent=MS.gene44464.t1
Gene Sequence

>MS.gene44464

ATGGATACGTTGTTTAGGCTTGTCAATTTTCAACAACAACAACAACAACAATATCAACCTGATCCTTCCTTCAACTCCACCACCACCTTAACAACTTCTAGCAGCTCTAGATCCTCAAGACAAACAACATATCAATATTACAACCAACAAGAAGAAGACGAAGAATGCTTCAACAACTTTTATTACATGGATCATAATAACAACAATGATGAAGATTTATCCTCATCTTCTTCTAAACAACACTATTATACTTATCCTTATGCATCCACTACTACTATCACTACTCCAAACACAACAAACACTACTTATAATACTATCAACACCCCCACCACAACTGATAATTACTCTTTCTCACCCTCCCACGATTACTTCAACTTTGAATTCTCCGGCAACTCATGGTCACAAAACATCCTCCTTGAAACCGCACGTGCATTTTCCGACAACAACACAAACCGAATCCAACAACTCATGTGGATGCTAAACGAGCTTAGTTCCCCATACGGTGACACAGATCAAAAACTTTCTTCTTATTTTCTTCAAGCTTTGTTTAGTCGCATGAACGACACGGGAGATAGAACCTACAAAACCTTAACATCCGCGTCGGAAAAAACATGTTCCTTCGATTCAACGAGGAAGATGTTGTTGAAGTTCCAAGAAGTTAGTCCATGGACAACGTTTGGACACGTGGCAGCCAACGGTGCTATCTTAGAAGCATTAGAAGGTAACCCTAAACTTCACATAATTGATATTAGCAACACTTATTGCACTCAATGGCCAACACTCCTCGAAGCGTTGGCCACCCGCTCCGATGATACGCCTCACCTCCGTTTAACCACTGTAGTCACCGCAGTCAGCGGTGGCTCGGTGCAAAAAGTCATGAAGGAAATCGGTTCAAGGATGGAAAAATTTGCTAGGCTCATGGGAGTGCCGTTTAAATTCAAAATCATTTTCAGTGATTTAAGAGAGTTGAATTTGTGTGATTTAGACATTAAAGAAGATGAAGCTTTGGCTATAAACTGTGTAAACTCTTTACATTCGATATCCGGAGCCGGAAACCACCGTGATTTGTTTATATCTTTGCTTCGTGGGTTAGAACCTAGGGTTTTAACTGTTGTTGAAGAGGAAGCTGATTTAGAAGTTTGTTTTGGTTCGGATTTCGTGGAAGGTTTTAAAGAGTGTTTAAGATGGTTTAGGGTTTATTTTGAAGCACTTGATGAAAGTTTTTCAAGAACAAGTAGTGAGAGGTTGATGCTAGAAAGAGAAGCTGGAAGAGGTATTGTTGATTTGGTTGCTTGTGATCCTTATGAGTCAATGGAGAGACGTGAAACAGCGTCACGGTGGAGGAGGAGGTTGCATGGTGGTGGGTTTAATACGGTGTCGTTTAGCGATGAAGTTTGTGATGATGTTAGGGCTTTGTTGAGAAGGTATAAAGAAGGGTGGTCGATGACACAGTGTTCCTCCGATGGTGATGCCGGAATATTCTTGTCGTGGAAGGATAAGCCGGTGGTGTGGGCCAGTGTATGGAGGCCTTGA
Protein sequence

>MS.gene44464.t1

MDTLFRLVNFQQQQQQQYQPDPSFNSTTTLTTSSSSRSSRQTTYQYYNQQEEDEECFNNFYYMDHNNNNDEDLSSSSSKQHYYTYPYASTTTITTPNTTNTTYNTINTPTTTDNYSFSPSHDYFNFEFSGNSWSQNILLETARAFSDNNTNRIQQLMWMLNELSSPYGDTDQKLSSYFLQALFSRMNDTGDRTYKTLTSASEKTCSFDSTRKMLLKFQEVSPWTTFGHVAANGAILEALEGNPKLHIIDISNTYCTQWPTLLEALATRSDDTPHLRLTTVVTAVSGGSVQKVMKEIGSRMEKFARLMGVPFKFKIIFSDLRELNLCDLDIKEDEALAINCVNSLHSISGAGNHRDLFISLLRGLEPRVLTVVEEEADLEVCFGSDFVEGFKECLRWFRVYFEALDESFSRTSSERLMLEREAGRGIVDLVACDPYESMERRETASRWRRRLHGGGFNTVSFSDEVCDDVRALLRRYKEGWSMTQCSSDGDAGIFLSWKDKPVVWASVWRP