Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44489.t1 | XP_003608464.1 | 98.8 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 3.90E-36 | 160.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44489.t1 | O64818 | 81.8 | 77 | 14 | 0 | 1 | 77 | 1 | 77 | 8.0e-30 | 130.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44489.t1 | G7JCT6 | 98.8 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 2.8e-36 | 160.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050320 | MS.gene44489 | 0.808575 | 2.89E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene44489 | 0.81538 | 9.52E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene44489 | 0.804945 | 1.69E-49 | -1.69E-46 |
| MS.gene051746 | MS.gene44489 | 0.800662 | 1.30E-48 | -1.69E-46 |
| MS.gene051748 | MS.gene44489 | 0.819635 | 1.05E-52 | -1.69E-46 |
| MS.gene051903 | MS.gene44489 | 0.803882 | 2.82E-49 | -1.69E-46 |
| MS.gene053109 | MS.gene44489 | 0.821356 | 4.22E-53 | -1.69E-46 |
| MS.gene054403 | MS.gene44489 | 0.81385 | 2.08E-51 | -1.69E-46 |
| MS.gene056752 | MS.gene44489 | 0.811983 | 5.33E-51 | -1.69E-46 |
| MS.gene057057 | MS.gene44489 | 0.805404 | 1.36E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene44489 | 0.812754 | 3.62E-51 | -1.69E-46 |
| MS.gene059171 | MS.gene44489 | 0.80823 | 3.43E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44489.t1 | MTR_4g095390 | 98.750 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 2.23e-54 | 163 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44489.t1 | AT2G23090 | 81.818 | 77 | 14 | 0 | 1 | 77 | 1 | 77 | 1.39e-43 | 135 |
Find 15 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGCTTCCCTTCATGCTTC+TGG | 0.193112 | 8.1:+25842096 | None:intergenic |
| CAAGTGAAGAGATCAGATTT+TGG | 0.307415 | 8.1:+25842135 | None:intergenic |
| CTCGAGAAGCAGAAGAATGC+TGG | 0.496068 | 8.1:-25843850 | MS.gene44489:CDS |
| ATCTTAAACCAGAAGCATGA+AGG | 0.503983 | 8.1:-25842104 | None:intergenic |
| CATGCTTCTGGTTTAAGATG+AGG | 0.513870 | 8.1:+25842108 | None:intergenic |
| CAGCATTCTTCTGCTTCTCG+AGG | 0.532542 | 8.1:+25843851 | None:intergenic |
| TCTCTCTCAGCCATGGGTGG+AGG | 0.540024 | 8.1:-25843910 | None:intergenic |
| TTGACCGTTACCTCCACCCA+TGG | 0.542711 | 8.1:+25843900 | None:intergenic |
| TCAGCCATGGGTGGAGGTAA+CGG | 0.548723 | 8.1:-25843904 | None:intergenic |
| GGGTCTCTCTCAGCCATGGG+TGG | 0.552601 | 8.1:-25843913 | None:intergenic |
| CTTGCACTTAACTTCTGATG+TGG | 0.570945 | 8.1:+25842179 | None:intergenic |
| AAGCAGAAGAATGCTGGCAA+AGG | 0.595797 | 8.1:-25843844 | MS.gene44489:intron |
| AAGCTAAGATGGCTCGCGAG+AGG | 0.616608 | 8.1:-25843876 | MS.gene44489:CDS |
| CACATCAGAAGTTAAGTGCA+AGG | 0.621035 | 8.1:-25842178 | MS.gene44489:CDS |
| TAAGTGCAAGGAGCATGCTG+AGG | 0.676702 | 8.1:-25842166 | MS.gene44489:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAAATTTATCATTACAA+CGG | - | chr8.1:25843687-25843706 | MS.gene44489:intron | 10.0% |
| !!! | CTATGTTTTATAAATTTTTA+AGG | + | chr8.1:25843182-25843201 | None:intergenic | 10.0% |
| !!! | TTTTTTTTTTTTGTTTCAAA+GGG | - | chr8.1:25842487-25842506 | MS.gene44489:intron | 10.0% |
| !!! | TTTTTTTTTTTTTGTTTCAA+AGG | - | chr8.1:25842486-25842505 | MS.gene44489:intron | 10.0% |
| !! | AAAATGGAAATTAGAAATTA+GGG | - | chr8.1:25842218-25842237 | MS.gene44489:intron | 15.0% |
| !! | AAACAAATTGATGAAATTAT+TGG | + | chr8.1:25842464-25842483 | None:intergenic | 15.0% |
| !! | ATGAAATTGAATAATATCAA+TGG | - | chr8.1:25842981-25843000 | MS.gene44489:intron | 15.0% |
| !!! | AATGAAAATTTACTTTTCAA+GGG | + | chr8.1:25842671-25842690 | None:intergenic | 15.0% |
| !! | AATATCAATGGTACTTAATT+TGG | - | chr8.1:25842993-25843012 | MS.gene44489:intron | 20.0% |
| !! | ACAACATTATAATCAGTAAA+GGG | + | chr8.1:25843760-25843779 | None:intergenic | 20.0% |
| !! | ACTTGAAATTCATTATGTTA+GGG | - | chr8.1:25842799-25842818 | MS.gene44489:intron | 20.0% |
| !! | CAAAATGGAAATTAGAAATT+AGG | - | chr8.1:25842217-25842236 | MS.gene44489:intron | 20.0% |
| !! | CAATAATGAATACTAATGTA+AGG | + | chr8.1:25843225-25843244 | None:intergenic | 20.0% |
| !! | TACTTGAAATTCATTATGTT+AGG | - | chr8.1:25842798-25842817 | MS.gene44489:intron | 20.0% |
| !! | TTGAATATTAAACCCAAAAA+TGG | - | chr8.1:25843066-25843085 | MS.gene44489:intron | 20.0% |
| !!! | AATTTCCATTTTGAAATTGT+CGG | + | chr8.1:25842210-25842229 | None:intergenic | 20.0% |
| !!! | ACACAAAATTATCCATTTTT+GGG | + | chr8.1:25843081-25843100 | None:intergenic | 20.0% |
| !!! | ACATATTGAAGAAAACTTTT+TGG | + | chr8.1:25843516-25843535 | None:intergenic | 20.0% |
| !!! | ACATATTGAATGAAACTTTT+TGG | + | chr8.1:25843617-25843636 | None:intergenic | 20.0% |
| !!! | CAATGAAAATTTACTTTTCA+AGG | + | chr8.1:25842672-25842691 | None:intergenic | 20.0% |
| !!! | CATATTGAAGAAAACTTTTT+GGG | + | chr8.1:25843515-25843534 | None:intergenic | 20.0% |
| !!! | CATATTGAATGAAACTTTTT+GGG | + | chr8.1:25843616-25843635 | None:intergenic | 20.0% |
| !!! | CTTGAAAAGTAAATTTTCAT+TGG | - | chr8.1:25842670-25842689 | MS.gene44489:intron | 20.0% |
| !!! | GATTTTTTCGTTGAAAAATT+GGG | - | chr8.1:25842244-25842263 | MS.gene44489:intron | 20.0% |
| !!! | TGATTTTTTCGTTGAAAAAT+TGG | - | chr8.1:25842243-25842262 | MS.gene44489:intron | 20.0% |
| !!! | TTTCATTGGTTAAACATTTT+GGG | - | chr8.1:25842684-25842703 | MS.gene44489:intron | 20.0% |
| !!! | TTTTCATTGGTTAAACATTT+TGG | - | chr8.1:25842683-25842702 | MS.gene44489:intron | 20.0% |
| ! | AAGTTTCATTCAATATGTCT+TGG | - | chr8.1:25843619-25843638 | MS.gene44489:intron | 25.0% |
| ! | CACAACATTATAATCAGTAA+AGG | + | chr8.1:25843761-25843780 | None:intergenic | 25.0% |
| ! | CAGAACTTTGATTGTAAAAT+TGG | - | chr8.1:25842595-25842614 | MS.gene44489:intron | 25.0% |
| ! | GAACAAGTTAAAGAATTTGA+GGG | - | chr8.1:25842883-25842902 | MS.gene44489:intron | 25.0% |
| ! | TGCCAAATATCAATATGTTT+GGG | + | chr8.1:25843256-25843275 | None:intergenic | 25.0% |
| ! | TTAAGAAATTCTGACTTCTT+TGG | - | chr8.1:25843118-25843137 | MS.gene44489:intron | 25.0% |
| ! | TTAATTATACGTGTTAGATC+TGG | - | chr8.1:25842282-25842301 | MS.gene44489:intron | 25.0% |
| ! | TTCCCAAACATATTGATATT+TGG | - | chr8.1:25843251-25843270 | MS.gene44489:intron | 25.0% |
| !! | CAAAAATGGATAATTTTGTG+TGG | - | chr8.1:25843080-25843099 | MS.gene44489:intron | 25.0% |
| !! | CACACAAAATTATCCATTTT+TGG | + | chr8.1:25843082-25843101 | None:intergenic | 25.0% |
| !! | TTATAGATCTGAAGTATATG+TGG | - | chr8.1:25842396-25842415 | MS.gene44489:intron | 25.0% |
| !! | TTATCATTACAACGGTTTTA+AGG | - | chr8.1:25843695-25843714 | MS.gene44489:intron | 25.0% |
| !!! | AATTTTTGTTTTGTACATGC+AGG | - | chr8.1:25842316-25842335 | MS.gene44489:intron | 25.0% |
| !!! | ATTTCCATTTTGAAATTGTC+GGG | + | chr8.1:25842209-25842228 | None:intergenic | 25.0% |
| !!! | CTTTTTTGTTTTTTTCAAGC+TGG | + | chr8.1:25842345-25842364 | None:intergenic | 25.0% |
| !!! | TATTTTGATTCCATGATGTT+TGG | - | chr8.1:25842722-25842741 | MS.gene44489:intron | 25.0% |
| AATTCATTATGTTAGGGAGT+TGG | - | chr8.1:25842805-25842824 | MS.gene44489:intron | 30.0% | |
| ACATATTGATATTTGGCACT+TGG | - | chr8.1:25843258-25843277 | MS.gene44489:intron | 30.0% | |
| AGAGATTAACAGAAGTTTCA+AGG | - | chr8.1:25842629-25842648 | MS.gene44489:intron | 30.0% | |
| ATAAAGGGAAAGGAATCTAA+GGG | - | chr8.1:25842841-25842860 | MS.gene44489:intron | 30.0% | |
| ATTAACAGAAGTTTCAAGGA+TGG | - | chr8.1:25842633-25842652 | MS.gene44489:intron | 30.0% | |
| ATTCATTATGTTAGGGAGTT+GGG | - | chr8.1:25842806-25842825 | MS.gene44489:intron | 30.0% | |
| CAAAAAAGCTATGTCAATTC+AGG | - | chr8.1:25842357-25842376 | MS.gene44489:intron | 30.0% | |
| CACAGTATGTAAGTTCATTT+CGG | - | chr8.1:25843311-25843330 | MS.gene44489:intron | 30.0% | |
| CGAACAAGTTAAAGAATTTG+AGG | - | chr8.1:25842882-25842901 | MS.gene44489:intron | 30.0% | |
| CTATTGTTAAGTTTCTACAG+AGG | + | chr8.1:25843042-25843061 | None:intergenic | 30.0% | |
| GATCAAACTGCAAAAATGTA+TGG | + | chr8.1:25842933-25842952 | None:intergenic | 30.0% | |
| GTGCCAAATATCAATATGTT+TGG | + | chr8.1:25843257-25843276 | None:intergenic | 30.0% | |
| TAGTCATTGAATATGCACAT+AGG | - | chr8.1:25843654-25843673 | MS.gene44489:CDS | 30.0% | |
| TATAAAGGGAAAGGAATCTA+AGG | - | chr8.1:25842840-25842859 | MS.gene44489:intron | 30.0% | |
| TGTAAGTTCATTACGAGTAT+TGG | - | chr8.1:25843360-25843379 | MS.gene44489:intron | 30.0% | |
| ! | AAGTGAAGAGATCAGATTTT+GGG | + | chr8.1:25843872-25843891 | None:intergenic | 30.0% |
| ! | TTGGCTTATGAGCATTTTAA+CGG | - | chr8.1:25842741-25842760 | MS.gene44489:intron | 30.0% |
| AAAACTGAGCCCCAAATTAA+GGG | + | chr8.1:25842528-25842547 | None:intergenic | 35.0% | |
| GAAATTCTGACTTCTTTGGT+TGG | - | chr8.1:25843122-25843141 | MS.gene44489:intron | 35.0% | |
| GGGAGAAAATATAAAGGGAA+AGG | - | chr8.1:25842831-25842850 | MS.gene44489:intron | 35.0% | |
| GGGTTGGGAGAAAATATAAA+GGG | - | chr8.1:25842826-25842845 | MS.gene44489:intron | 35.0% | |
| TAAAACTGAGCCCCAAATTA+AGG | + | chr8.1:25842529-25842548 | None:intergenic | 35.0% | |
| TAAAGGGAAAGGAATCTAAG+GGG | - | chr8.1:25842842-25842861 | MS.gene44489:intron | 35.0% | |
| TAACGGTCAAAAAGCTAAGA+TGG | - | chr8.1:25842118-25842137 | MS.gene44489:CDS | 35.0% | |
| TGTTCCCGACAATTTCAAAA+TGG | - | chr8.1:25842202-25842221 | MS.gene44489:CDS | 35.0% | |
| TGTTTGGATCCCCTTAATTT+GGG | - | chr8.1:25842515-25842534 | MS.gene44489:intron | 35.0% | |
| ! | CAAGTGAAGAGATCAGATTT+TGG | + | chr8.1:25843873-25843892 | None:intergenic | 35.0% |
| ! | GTTTGACAAGTTCTGTTTTG+AGG | - | chr8.1:25843437-25843456 | MS.gene44489:intron | 35.0% |
| ! | TAAGTTTCTACAGAGGCTTT+TGG | + | chr8.1:25843035-25843054 | None:intergenic | 35.0% |
| ! | TGGGTTGGGAGAAAATATAA+AGG | - | chr8.1:25842825-25842844 | MS.gene44489:intron | 35.0% |
| !! | ACAATCAAAGTTCTGAACAG+AGG | + | chr8.1:25842590-25842609 | None:intergenic | 35.0% |
| AAACTGAGCCCCAAATTAAG+GGG | + | chr8.1:25842527-25842546 | None:intergenic | 40.0% | |
| AGCAACATACAGACTGTAGT+TGG | - | chr8.1:25843415-25843434 | MS.gene44489:intron | 40.0% | |
| ATTATGTTAGGGAGTTGGGT+TGG | - | chr8.1:25842810-25842829 | MS.gene44489:intron | 40.0% | |
| CACATCAGAAGTTAAGTGCA+AGG | - | chr8.1:25843827-25843846 | MS.gene44489:intron | 40.0% | |
| CTGTTTGGATCCCCTTAATT+TGG | - | chr8.1:25842514-25842533 | MS.gene44489:intron | 40.0% | |
| CTTGCACTTAACTTCTGATG+TGG | + | chr8.1:25843829-25843848 | None:intergenic | 40.0% | |
| GCATTATGCAGTTTACACGA+TGG | + | chr8.1:25843482-25843501 | None:intergenic | 40.0% | |
| GTACTATGCAGTTTACACAG+TGG | + | chr8.1:25843586-25843605 | None:intergenic | 40.0% | |
| GTTTCAAAGGGTTCTCTGTT+TGG | - | chr8.1:25842499-25842518 | MS.gene44489:intron | 40.0% | |
| TGCATTGCTGTAGTATGGAA+TGG | - | chr8.1:25843732-25843751 | MS.gene44489:intron | 40.0% | |
| TGCTCATAAGCCAAACATCA+TGG | + | chr8.1:25842735-25842754 | None:intergenic | 40.0% | |
| TGTAAGTTCATTTCGGTGAC+TGG | - | chr8.1:25843318-25843337 | MS.gene44489:intron | 40.0% | |
| TTATGTTAGGGAGTTGGGTT+GGG | - | chr8.1:25842811-25842830 | MS.gene44489:intron | 40.0% | |
| ! | CTTCTTTGGTTGGCGTATTT+TGG | - | chr8.1:25843132-25843151 | MS.gene44489:intron | 40.0% |
| ! | GTTTGGATCCCCTTAATTTG+GGG | - | chr8.1:25842516-25842535 | MS.gene44489:intron | 40.0% |
| ! | TGATTGATGATTGCAGTGCA+AGG | - | chr8.1:25843779-25843798 | MS.gene44489:intron | 40.0% |
| !! | CATGCTTCTGGTTTAAGATG+AGG | + | chr8.1:25843900-25843919 | None:intergenic | 40.0% |
| !! | GCTTATGAGCATTTTAACGG+AGG | - | chr8.1:25842744-25842763 | MS.gene44489:intron | 40.0% |
| AAGCAGAAGAATGCTGGCAA+AGG | - | chr8.1:25842161-25842180 | MS.gene44489:CDS | 45.0% | |
| GTCACTGCATTGCTGTAGTA+TGG | - | chr8.1:25843727-25843746 | MS.gene44489:intron | 45.0% | |
| !! | GAGATCAGATTTTGGGTGTC+TGG | + | chr8.1:25843865-25843884 | None:intergenic | 45.0% |
| !!! | TATGTTTTATAAATTTTTAA+GGG | + | chr8.1:25843181-25843200 | None:intergenic | 5.0% |
| CAGCATTCTTCTGCTTCTCG+AGG | + | chr8.1:25842157-25842176 | None:intergenic | 50.0% | |
| CTCGAGAAGCAGAAGAATGC+TGG | - | chr8.1:25842155-25842174 | MS.gene44489:CDS | 50.0% | |
| TGTCGGGAACAGAGAGAGAA+TGG | + | chr8.1:25842193-25842212 | None:intergenic | 50.0% | |
| ! | TAAGTGCAAGGAGCATGCTG+AGG | - | chr8.1:25843839-25843858 | MS.gene44489:intron | 50.0% |
| AAGCTAAGATGGCTCGCGAG+AGG | - | chr8.1:25842129-25842148 | MS.gene44489:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 25842107 | 25843920 | 25842107 | ID=MS.gene44489 |
| chr8.1 | mRNA | 25842107 | 25843920 | 25842107 | ID=MS.gene44489.t1;Parent=MS.gene44489 |
| chr8.1 | exon | 25843845 | 25843920 | 25843845 | ID=MS.gene44489.t1.exon1;Parent=MS.gene44489.t1 |
| chr8.1 | CDS | 25843845 | 25843920 | 25843845 | ID=cds.MS.gene44489.t1;Parent=MS.gene44489.t1 |
| chr8.1 | exon | 25843649 | 25843689 | 25843649 | ID=MS.gene44489.t1.exon2;Parent=MS.gene44489.t1 |
| chr8.1 | CDS | 25843649 | 25843689 | 25843649 | ID=cds.MS.gene44489.t1;Parent=MS.gene44489.t1 |
| chr8.1 | exon | 25842107 | 25842232 | 25842107 | ID=MS.gene44489.t1.exon3;Parent=MS.gene44489.t1 |
| chr8.1 | CDS | 25842107 | 25842232 | 25842107 | ID=cds.MS.gene44489.t1;Parent=MS.gene44489.t1 |
| Gene Sequence |
| Protein sequence |
