AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44826

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44826.t1 MTR_5g068860 95.538 493 19 1 1 490 19 511 0.0 931
MS.gene44826.t1 MTR_1g115415 53.250 400 185 1 82 481 71 468 6.15e-155 451
MS.gene44826.t1 MTR_8g105560 43.128 422 184 4 82 502 49 415 1.60e-112 345
MS.gene44826.t1 MTR_4g070060 27.606 355 249 4 115 463 235 587 1.42e-34 137
MS.gene44826.t1 MTR_4g084630 23.548 310 199 4 155 462 46 319 2.95e-31 124
MS.gene44826.t1 MTR_4g084630 23.182 220 160 3 120 330 43 262 6.48e-17 82.4
MS.gene44826.t1 MTR_2g034600 28.425 292 199 7 185 473 210 494 1.12e-29 122
MS.gene44826.t1 MTR_2g034600 21.986 282 171 7 118 354 211 488 3.23e-13 72.4
MS.gene44826.t1 MTR_2g034600 28.425 292 199 7 185 473 275 559 1.90e-29 122
MS.gene44826.t1 MTR_2g034600 21.986 282 171 7 118 354 276 553 3.00e-13 72.4
MS.gene44826.t1 MTR_2g034600 28.425 292 199 7 185 473 271 555 2.19e-29 122
MS.gene44826.t1 MTR_2g034600 21.986 282 171 7 118 354 272 549 3.55e-13 72.4
MS.gene44826.t1 MTR_5g094610 25.982 331 196 9 150 471 191 481 5.86e-25 108
MS.gene44826.t1 MTR_5g094610 24.153 236 166 5 113 340 221 451 4.75e-12 68.6
MS.gene44826.t1 MTR_6g003960 24.924 329 241 6 135 461 263 587 5.02e-23 103
MS.gene44826.t1 MTR_2g049780 25.379 264 156 7 202 463 82 306 4.47e-17 82.4
MS.gene44826.t1 MTR_5g041630 25.461 271 158 7 190 458 61 289 5.97e-14 73.2
MS.gene44826.t1 MTR_2g067230 23.022 278 167 6 190 466 73 304 1.08e-13 72.8
MS.gene44826.t1 MTR_7g081270 23.774 265 182 7 206 461 26 279 8.10e-12 66.2
MS.gene44826.t1 MTR_3g092710 22.604 407 266 14 98 461 91 491 3.36e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44826.t1 AT4G02990 72.473 465 119 2 34 489 55 519 0.0 675
MS.gene44826.t1 AT4G02990 72.473 465 119 2 34 489 55 519 0.0 675
MS.gene44826.t1 AT2G44020 49.876 403 200 1 82 484 76 476 2.89e-145 427
MS.gene44826.t1 AT2G21710 26.854 391 276 7 114 499 241 626 8.54e-40 153
MS.gene44826.t1 AT2G21710 24.460 278 194 6 81 343 274 550 9.81e-18 87.0
MS.gene44826.t1 AT4G38160 24.638 276 205 3 198 470 54 329 2.08e-32 127
MS.gene44826.t1 AT4G38160 20.705 227 167 5 116 330 39 264 2.40e-14 74.7
MS.gene44826.t1 AT4G38160 24.638 276 205 3 198 470 54 329 2.08e-32 127
MS.gene44826.t1 AT4G38160 20.705 227 167 5 116 330 39 264 2.40e-14 74.7
MS.gene44826.t1 AT4G38160 24.638 276 205 3 198 470 54 329 2.77e-32 127
MS.gene44826.t1 AT4G38160 20.705 227 167 5 116 330 39 264 1.91e-14 75.5
MS.gene44826.t1 AT4G38160 24.638 276 205 3 198 470 54 329 6.28e-32 126
MS.gene44826.t1 AT4G38160 20.705 227 167 5 116 330 39 264 4.07e-14 74.3
MS.gene44826.t1 AT4G14605 29.434 265 177 7 201 462 211 468 3.99e-27 114
MS.gene44826.t1 AT4G14605 29.434 265 177 7 201 462 211 468 3.99e-27 114
MS.gene44826.t1 AT2G21710 25.182 274 203 2 153 425 284 556 3.50e-26 112
MS.gene44826.t1 AT2G21710 24.460 278 194 6 81 343 274 550 1.41e-17 86.3
MS.gene44826.t1 AT2G21710 25.368 272 201 2 153 423 284 554 4.21e-26 112
MS.gene44826.t1 AT2G21710 24.460 278 194 6 81 343 274 550 1.05e-17 86.7
MS.gene44826.t1 AT1G78930 23.404 329 247 5 135 461 249 574 7.66e-25 108
MS.gene44826.t1 AT5G55580 23.529 306 223 7 143 443 38 337 4.98e-20 92.4
MS.gene44826.t1 AT5G55580 23.932 234 167 5 111 337 106 335 1.48e-12 69.7
MS.gene44826.t1 AT5G55580 24.036 337 242 9 143 471 144 474 1.40e-19 92.0
MS.gene44826.t1 AT5G55580 23.932 234 167 5 111 337 212 441 3.18e-12 69.3
MS.gene44826.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene44826.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene44826.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene44826.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene44826.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene44826.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene44826.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene44826.t1 AT2G34620 26.006 323 193 7 145 463 12 292 5.56e-17 82.0
MS.gene44826.t1 AT5G55580 23.711 194 141 4 151 340 185 375 6.56e-14 73.9
MS.gene44826.t1 AT1G62120 22.378 286 186 6 189 441 91 373 4.61e-13 71.6
MS.gene44826.t1 AT1G61980 22.930 314 201 9 189 464 87 397 1.64e-12 69.7
MS.gene44826.t1 AT1G61980 22.930 314 201 9 189 464 87 397 1.64e-12 69.7
MS.gene44826.t1 AT1G61980 22.930 314 201 9 189 464 87 397 1.64e-12 69.7
MS.gene44826.t1 AT3G18870 27.517 149 104 2 314 461 123 268 5.16e-12 67.0
MS.gene44826.t1 AT2G03050 24.510 204 149 5 265 465 71 272 3.10e-11 64.7
MS.gene44826.t1 AT2G03050 24.510 204 149 5 265 465 72 273 3.28e-11 64.7
MS.gene44826.t1 AT5G54180 24.051 395 232 11 94 466 145 493 5.90e-11 65.1

Find 128 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTAGATTCCCGGAGATTT+TGG 0.122651 5.2:-58404929 MS.gene44826:CDS
GCTTGATTACCTTGGTAAAT+TGG 0.181028 5.2:-58405199 MS.gene44826:CDS
GAGACACAACCTTATATTCT+TGG 0.218625 5.2:-58404820 MS.gene44826:CDS
AATTGCCATGTAATTGTATT+AGG 0.259846 5.2:-58405602 MS.gene44826:CDS
TTCCCAGCATTCTTCACTTA+TGG 0.279890 5.2:-58404394 MS.gene44826:CDS
GGAAAGGCCTTTGGTAGATT+TGG 0.285905 5.2:-58404422 MS.gene44826:CDS
TTATATTCTTGGATTCGATT+TGG 0.301999 5.2:-58404809 MS.gene44826:CDS
ACATCTGTTGCTTATTTGAT+TGG 0.305827 5.2:-58404988 MS.gene44826:CDS
GCTCAATTCTGTGCTTGATT+TGG 0.309060 5.2:-58404650 MS.gene44826:CDS
AGCATTCTTCACTTATGGTT+TGG 0.313050 5.2:-58404389 MS.gene44826:CDS
AGTTTGAATCCCAACACTTC+AGG 0.346326 5.2:+58405027 None:intergenic
GCCGGTGGTCAAGTATCTTC+AGG 0.347359 5.2:-58405097 MS.gene44826:CDS
TTCATAACATCAACCGTAAC+AGG 0.355753 5.2:+58405324 None:intergenic
GAAATGCCAATTCTAACATT+TGG 0.356044 5.2:-58405660 MS.gene44826:CDS
GAATGACCAAATGTTAGAAT+TGG 0.365180 5.2:+58405654 None:intergenic
GGACTATGACACTATTGATA+TGG 0.372055 5.2:-58404263 MS.gene44826:CDS
GATTTGGATGTTGAGGATTT+CGG 0.372881 5.2:-58404634 MS.gene44826:CDS
AAGATTCAATGTTCTGTTGC+TGG 0.373247 5.2:-58405561 MS.gene44826:CDS
ACCTTGGTAAATTGGGAGTT+AGG 0.378790 5.2:-58405191 MS.gene44826:CDS
CTTGATTACCTTGGTAAATT+GGG 0.382917 5.2:-58405198 MS.gene44826:CDS
AAATCGAATCCAAGAATATA+AGG 0.400009 5.2:+58404811 None:intergenic
ACCCGCATCCCCAAAATCTC+CGG 0.400611 5.2:+58404919 None:intergenic
TAACAACTATCCTCTTGTAC+TGG 0.404481 5.2:-58405253 MS.gene44826:CDS
TGGCGAAATTCAAAGGAAAC+GGG 0.417191 5.2:-58405481 MS.gene44826:CDS
AGGCATCTTAACTAGATTCC+CGG 0.419846 5.2:-58404938 MS.gene44826:CDS
TGATATGGAAGAGATGGAAA+TGG 0.426007 5.2:-58404248 MS.gene44826:CDS
TATGAGTTCTTGCGAGGAAT+CGG 0.426180 5.2:-58405381 MS.gene44826:CDS
ATGTAGTGTCAAGAAGAATA+TGG 0.433633 5.2:-58405229 MS.gene44826:CDS
GACCGGTGCTGCTGATTCGC+GGG 0.440005 5.2:-58405520 MS.gene44826:CDS
TATCTGCAAGCTTCGGCTCT+AGG 0.443828 5.2:+58404683 None:intergenic
GTAATTGTATTAGGAAGATA+AGG 0.444282 5.2:-58405593 MS.gene44826:CDS
GTGGAGTATCTGGAAAGCTT+GGG 0.446638 5.2:-58404874 MS.gene44826:CDS
GTTGCTTATTTGATTGGAAT+CGG 0.449333 5.2:-58404982 MS.gene44826:CDS
GCTCAGTATCCTGACATCAT+TGG 0.449340 5.2:-58404712 MS.gene44826:CDS
TCAAGACCATCAAGTTCATC+AGG 0.450633 5.2:+58405351 None:intergenic
TGTGGAGTATCTGGAAAGCT+TGG 0.452920 5.2:-58404875 MS.gene44826:CDS
CTGGTAGGACGTATAGTTAT+AGG 0.454647 5.2:-58405542 MS.gene44826:CDS
ATTGTTCCTGATGAACTTGA+TGG 0.456166 5.2:-58405357 MS.gene44826:CDS
AATTTCGCCAGAGTCCCGTT+TGG 0.461902 5.2:+58405494 None:intergenic
CTGGCGAAATTCAAAGGAAA+CGG 0.464517 5.2:-58405482 MS.gene44826:CDS
CCGGTGGTCAAGTATCTTCA+GGG 0.466032 5.2:-58405096 MS.gene44826:CDS
CGGGGATCCAAACGGGACTC+TGG 0.468895 5.2:-58405501 MS.gene44826:CDS
TGATTCGCGGGGATCCAAAC+GGG 0.468998 5.2:-58405508 MS.gene44826:CDS
GAGAGGTATCCTGAAGTGTT+GGG 0.470423 5.2:-58405036 MS.gene44826:CDS
CACTATTGATATGGAAGAGA+TGG 0.470517 5.2:-58404254 MS.gene44826:CDS
GCCGAACATTAAACTCTTCG+AGG 0.470544 5.2:+58404758 None:intergenic
CTTTCCAGATACTCCACAAA+AGG 0.473709 5.2:+58404880 None:intergenic
CTTCTCTTATCTGCAAGCTT+CGG 0.475890 5.2:+58404676 None:intergenic
CATAACATGGTTGCTAAGAA+AGG 0.477153 5.2:-58404346 MS.gene44826:CDS
GCTTCATTCTAGTGTGGTTG+TGG 0.477857 5.2:-58405127 MS.gene44826:CDS
AAACCATAAGTGAAGAATGC+TGG 0.479920 5.2:+58404391 None:intergenic
GAAAGGATTGAAGTGTTCTC+TGG 0.480186 5.2:-58404329 MS.gene44826:CDS
GGACCGGTGCTGCTGATTCG+CGG 0.485919 5.2:-58405521 MS.gene44826:CDS
CCCTCGAAGAGTTTAATGTT+CGG 0.492782 5.2:-58404759 MS.gene44826:CDS
CTGATTCGCGGGGATCCAAA+CGG 0.493660 5.2:-58405509 MS.gene44826:CDS
CAAATGAGGAAGATGATTGT+TGG 0.494500 5.2:-58404514 MS.gene44826:CDS
GTGTTGGGATTCAAACTTGA+GGG 0.498220 5.2:-58405021 MS.gene44826:CDS
AAGTGAGATGGAAAGGCCTT+TGG 0.499414 5.2:-58404431 MS.gene44826:CDS
ATCTGAAATTAAGAAAGATA+AGG 0.501088 5.2:-58405424 MS.gene44826:CDS
AATGAATTCATGTCAAATGA+TGG 0.505787 5.2:+58404223 None:intergenic
GTTCCTGTGCTTGATTACCT+TGG 0.506625 5.2:-58405207 MS.gene44826:CDS
TCAGGGGATGGATATCAAAC+CGG 0.510690 5.2:-58405079 MS.gene44826:CDS
AAGGCCTTTCCATCTCACTT+TGG 0.510909 5.2:+58404434 None:intergenic
TGAGAGGTATCCTGAAGTGT+TGG 0.512556 5.2:-58405037 MS.gene44826:CDS
CCACGTTAACGCAGTTCTTG+CGG 0.514225 5.2:-58405164 MS.gene44826:CDS
TGTTACGGTTGATGTTATGA+AGG 0.514841 5.2:-58405322 MS.gene44826:CDS
TGGTCTTGAACTTCCTGTTA+CGG 0.514972 5.2:-58405337 MS.gene44826:CDS
GAATCTGATTCATCGCTCCT+TGG 0.517997 5.2:+58404193 None:intergenic
TTAGCAACCATGTTATGCCT+AGG 0.519862 5.2:+58404352 None:intergenic
AGTCTAGCAATAGCTAGCCT+TGG 0.521140 5.2:+58404847 None:intergenic
TGTTTAAAGCAAGTAGTTGA+GGG 0.521469 5.2:+58404488 None:intergenic
TGTGCTTGATTTGGATGTTG+AGG 0.522040 5.2:-58404641 MS.gene44826:CDS
AGGTCGGTTCCAATGATGTC+AGG 0.523721 5.2:+58404703 None:intergenic
AACCATAAGTGAAGAATGCT+GGG 0.531139 5.2:+58404392 None:intergenic
AATTTCAGATAAACTCGGAC+GGG 0.532219 5.2:+58405437 None:intergenic
CCCGCATCCCCAAAATCTCC+GGG 0.534445 5.2:+58404920 None:intergenic
ATCTTCCTAATACAATTACA+TGG 0.538869 5.2:+58405597 None:intergenic
TCCCCGCGAATCAGCAGCAC+CGG 0.539690 5.2:+58405518 None:intergenic
TGCAAGCTTCGGCTCTAGGT+CGG 0.547317 5.2:+58404687 None:intergenic
AATTCAAAGGAAACGGGGAG+GGG 0.548403 5.2:-58405475 MS.gene44826:CDS
CGATTACTTCCAAAGTGAGA+TGG 0.549323 5.2:-58404443 MS.gene44826:CDS
GGGACTCTGGCGAAATTCAA+AGG 0.550251 5.2:-58405488 MS.gene44826:CDS
TTCTTAATTTCAGATAAACT+CGG 0.551846 5.2:+58405432 None:intergenic
AAATTCAAAGGAAACGGGGA+GGG 0.552308 5.2:-58405476 MS.gene44826:CDS
TATTTGATTGGAATCGGTGT+TGG 0.555605 5.2:-58404976 MS.gene44826:CDS
ATGTTTAAAGCAAGTAGTTG+AGG 0.555715 5.2:+58404487 None:intergenic
CCCTGAAGATACTTGACCAC+CGG 0.559238 5.2:+58405096 None:intergenic
AACAACTATCCTCTTGTACT+GGG 0.560648 5.2:-58405252 MS.gene44826:CDS
CCGCAAGAACTGCGTTAACG+TGG 0.564848 5.2:+58405164 None:intergenic
AGGATACTGAGCAATTATAG+AGG 0.570014 5.2:+58404723 None:intergenic
ACACTAGAATGAAGCACTTG+TGG 0.573884 5.2:+58405135 None:intergenic
AAATCAACGTGCTTCAGCAT+AGG 0.578326 5.2:+58404565 None:intergenic
ACACTACATCCCAGTACAAG+AGG 0.579075 5.2:+58405243 None:intergenic
GGTCAAGTATCTTCAGGGGA+TGG 0.581823 5.2:-58405091 MS.gene44826:CDS
TGTGGTTGTGGATCTTGTGC+CGG 0.581899 5.2:-58405115 MS.gene44826:CDS
ACTTCCAAAGTGAGATGGAA+AGG 0.582725 5.2:-58404438 MS.gene44826:CDS
TGAATTCATTGATGCGGCCA+AGG 0.585206 5.2:-58404210 MS.gene44826:CDS
TTGACATGAATTCATTGATG+CGG 0.585388 5.2:-58404216 MS.gene44826:CDS
ATATTCCTCGTGTTCTTGAG+AGG 0.588108 5.2:-58405053 MS.gene44826:CDS
TGAGAAATTTGAGCAACGAA+TGG 0.590339 5.2:-58404284 MS.gene44826:CDS
AGAACACGAGGAATATCATC+CGG 0.593566 5.2:+58405060 None:intergenic
GATTGAAGTGTTCTCTGGCA+TGG 0.595640 5.2:-58404324 MS.gene44826:CDS
ATAAGCAACAGATGTACTCA+TGG 0.595928 5.2:+58404996 None:intergenic
TCCTAACTCCCAATTTACCA+AGG 0.597942 5.2:+58405190 None:intergenic
CCGAACATTAAACTCTTCGA+GGG 0.604717 5.2:+58404759 None:intergenic
AGGACGTATAGTTATAGGAC+CGG 0.612646 5.2:-58405537 MS.gene44826:CDS
TTACCAAGGTAATCAAGCAC+AGG 0.615617 5.2:+58405204 None:intergenic
TTCAATGTTCTGTTGCTGGT+AGG 0.617163 5.2:-58405557 MS.gene44826:CDS
TGGAAAGCTTGGGTATTCCA+AGG 0.618103 5.2:-58404864 MS.gene44826:CDS
AACTCAACCAAATCTACCAA+AGG 0.619874 5.2:+58404415 None:intergenic
TAATTTCAGATAAACTCGGA+CGG 0.623058 5.2:+58405436 None:intergenic
ACAAGTGCTTCATTCTAGTG+TGG 0.623981 5.2:-58405133 MS.gene44826:CDS
ATTGTTTATGAGTTCTTGCG+AGG 0.628501 5.2:-58405387 MS.gene44826:CDS
TGGAGTCAACTATAAAACCT+AGG 0.628708 5.2:-58404369 MS.gene44826:CDS
ACCGGTGCTGCTGATTCGCG+GGG 0.640590 5.2:-58405519 MS.gene44826:CDS
TATAAAACCTAGGCATAACA+TGG 0.645601 5.2:-58404359 MS.gene44826:CDS
AATCATTGAGAAGATGCCGC+AGG 0.648015 5.2:-58404608 MS.gene44826:CDS
GGTGTTGGAAGAAGAGAACT+CGG 0.656801 5.2:-58404961 MS.gene44826:CDS
CGGTGGTCAAGTATCTTCAG+GGG 0.659657 5.2:-58405095 MS.gene44826:CDS
AGTGTTGGGATTCAAACTTG+AGG 0.668806 5.2:-58405022 MS.gene44826:CDS
GGTTGTGGATCTTGTGCCGG+TGG 0.671700 5.2:-58405112 MS.gene44826:CDS
GAAATTCAAAGGAAACGGGG+AGG 0.688989 5.2:-58405477 MS.gene44826:CDS
TCAGCATAGGTGTAGTACTG+AGG 0.702066 5.2:+58404578 None:intergenic
ATAAACAATTTCACGCAACG+TGG 0.704806 5.2:+58405401 None:intergenic
GGCGAAATTCAAAGGAAACG+GGG 0.719631 5.2:-58405480 MS.gene44826:CDS
GTACTGAGGCTAACTACCTG+CGG 0.724109 5.2:+58404592 None:intergenic
GGATACCTCTCAAGAACACG+AGG 0.733931 5.2:+58405048 None:intergenic
GTTGGAAGAAGAGAACTCGG+AGG 0.795387 5.2:-58404958 MS.gene44826:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTTAATTTCAGATAAACT+CGG + chr5.2:58404366-58404385 None:intergenic 20.0%
!!! ATCTGAAATTAAGAAAGATA+AGG - chr5.2:58404371-58404390 MS.gene44826:CDS 20.0%
! AAATCGAATCCAAGAATATA+AGG + chr5.2:58404987-58405006 None:intergenic 25.0%
! AATGAATTCATGTCAAATGA+TGG + chr5.2:58405575-58405594 None:intergenic 25.0%
! AATTGCCATGTAATTGTATT+AGG - chr5.2:58404193-58404212 MS.gene44826:CDS 25.0%
! ATCTTCCTAATACAATTACA+TGG + chr5.2:58404201-58404220 None:intergenic 25.0%
! GTAATTGTATTAGGAAGATA+AGG - chr5.2:58404202-58404221 MS.gene44826:CDS 25.0%
! TTATATTCTTGGATTCGATT+TGG - chr5.2:58404986-58405005 MS.gene44826:CDS 25.0%
!! TTGATTTTCTTAAGGATTGT+GGG - chr5.2:58405246-58405265 MS.gene44826:CDS 25.0%
ATGTAGTGTCAAGAAGAATA+TGG - chr5.2:58404566-58404585 MS.gene44826:CDS 30.0%
CTTGATTACCTTGGTAAATT+GGG - chr5.2:58404597-58404616 MS.gene44826:CDS 30.0%
GAATGACCAAATGTTAGAAT+TGG + chr5.2:58404144-58404163 None:intergenic 30.0%
GTTGCTTATTTGATTGGAAT+CGG - chr5.2:58404813-58404832 MS.gene44826:CDS 30.0%
TAATTTCAGATAAACTCGGA+CGG + chr5.2:58404362-58404381 None:intergenic 30.0%
TATAAAACCTAGGCATAACA+TGG - chr5.2:58405436-58405455 MS.gene44826:CDS 30.0%
TTGACATGAATTCATTGATG+CGG - chr5.2:58405579-58405598 MS.gene44826:CDS 30.0%
! ACATCTGTTGCTTATTTGAT+TGG - chr5.2:58404807-58404826 MS.gene44826:CDS 30.0%
! ATGTTTAAAGCAAGTAGTTG+AGG + chr5.2:58405311-58405330 None:intergenic 30.0%
! TGTTTAAAGCAAGTAGTTGA+GGG + chr5.2:58405310-58405329 None:intergenic 30.0%
! TTGATTTTCTTCACAGTTTG+GGG - chr5.2:58404502-58404521 MS.gene44826:CDS 30.0%
!! GTTGATTTTCTTAAGGATTG+TGG - chr5.2:58405245-58405264 MS.gene44826:CDS 30.0%
!! GTTGATTTTCTTCACAGTTT+GGG - chr5.2:58404501-58404520 MS.gene44826:CDS 30.0%
!!! TGTTGATTTTCTTCACAGTT+TGG - chr5.2:58404500-58404519 MS.gene44826:CDS 30.0%
AAACCATAAGTGAAGAATGC+TGG + chr5.2:58405407-58405426 None:intergenic 35.0%
AACAACTATCCTCTTGTACT+GGG - chr5.2:58404543-58404562 MS.gene44826:CDS 35.0%
AACCATAAGTGAAGAATGCT+GGG + chr5.2:58405406-58405425 None:intergenic 35.0%
AACTCAACCAAATCTACCAA+AGG + chr5.2:58405383-58405402 None:intergenic 35.0%
AAGATTCAATGTTCTGTTGC+TGG - chr5.2:58404234-58404253 MS.gene44826:CDS 35.0%
AATTTCAGATAAACTCGGAC+GGG + chr5.2:58404361-58404380 None:intergenic 35.0%
AGCATTCTTCACTTATGGTT+TGG - chr5.2:58405406-58405425 MS.gene44826:CDS 35.0%
AGGATACTGAGCAATTATAG+AGG + chr5.2:58405075-58405094 None:intergenic 35.0%
ATAAACAATTTCACGCAACG+TGG + chr5.2:58404397-58404416 None:intergenic 35.0%
ATAAGCAACAGATGTACTCA+TGG + chr5.2:58404802-58404821 None:intergenic 35.0%
ATTGTTCCTGATGAACTTGA+TGG - chr5.2:58404438-58404457 MS.gene44826:CDS 35.0%
ATTGTTTATGAGTTCTTGCG+AGG - chr5.2:58404408-58404427 MS.gene44826:CDS 35.0%
CAAATGAGGAAGATGATTGT+TGG - chr5.2:58405281-58405300 MS.gene44826:CDS 35.0%
CACTATTGATATGGAAGAGA+TGG - chr5.2:58405541-58405560 MS.gene44826:CDS 35.0%
CATAACATGGTTGCTAAGAA+AGG - chr5.2:58405449-58405468 MS.gene44826:CDS 35.0%
GAGACACAACCTTATATTCT+TGG - chr5.2:58404975-58404994 MS.gene44826:CDS 35.0%
GATTTGGATGTTGAGGATTT+CGG - chr5.2:58405161-58405180 MS.gene44826:CDS 35.0%
GCTTGATTACCTTGGTAAAT+TGG - chr5.2:58404596-58404615 MS.gene44826:CDS 35.0%
GGACTATGACACTATTGATA+TGG - chr5.2:58405532-58405551 MS.gene44826:CDS 35.0%
TAACAACTATCCTCTTGTAC+TGG - chr5.2:58404542-58404561 MS.gene44826:CDS 35.0%
TATTTGATTGGAATCGGTGT+TGG - chr5.2:58404819-58404838 MS.gene44826:CDS 35.0%
TGAGAAATTTGAGCAACGAA+TGG - chr5.2:58405511-58405530 MS.gene44826:CDS 35.0%
TGATATGGAAGAGATGGAAA+TGG - chr5.2:58405547-58405566 MS.gene44826:CDS 35.0%
TGGAGTCAACTATAAAACCT+AGG - chr5.2:58405426-58405445 MS.gene44826:CDS 35.0%
TTCATAACATCAACCGTAAC+AGG + chr5.2:58404474-58404493 None:intergenic 35.0%
! TGTTACGGTTGATGTTATGA+AGG - chr5.2:58404473-58404492 MS.gene44826:CDS 35.0%
!!! GAAGCACGTTGATTTTCTTA+AGG - chr5.2:58405238-58405257 MS.gene44826:CDS 35.0%
AAATCAACGTGCTTCAGCAT+AGG + chr5.2:58405233-58405252 None:intergenic 40.0%
AAATTCAAAGGAAACGGGGA+GGG - chr5.2:58404319-58404338 MS.gene44826:CDS 40.0%
ACAAGTGCTTCATTCTAGTG+TGG - chr5.2:58404662-58404681 MS.gene44826:CDS 40.0%
ACCTTGGTAAATTGGGAGTT+AGG - chr5.2:58404604-58404623 MS.gene44826:CDS 40.0%
ACTTCCAAAGTGAGATGGAA+AGG - chr5.2:58405357-58405376 MS.gene44826:CDS 40.0%
AGAACACGAGGAATATCATC+CGG + chr5.2:58404738-58404757 None:intergenic 40.0%
AGGACGTATAGTTATAGGAC+CGG - chr5.2:58404258-58404277 MS.gene44826:CDS 40.0%
AGGCATCTTAACTAGATTCC+CGG - chr5.2:58404857-58404876 MS.gene44826:CDS 40.0%
AGTTTGAATCCCAACACTTC+AGG + chr5.2:58404771-58404790 None:intergenic 40.0%
ATATTCCTCGTGTTCTTGAG+AGG - chr5.2:58404742-58404761 MS.gene44826:CDS 40.0%
CCCTCGAAGAGTTTAATGTT+CGG - chr5.2:58405036-58405055 MS.gene44826:CDS 40.0%
CCGAACATTAAACTCTTCGA+GGG + chr5.2:58405039-58405058 None:intergenic 40.0%
CGATTACTTCCAAAGTGAGA+TGG - chr5.2:58405352-58405371 MS.gene44826:CDS 40.0%
CTGGCGAAATTCAAAGGAAA+CGG - chr5.2:58404313-58404332 MS.gene44826:CDS 40.0%
CTGGTAGGACGTATAGTTAT+AGG - chr5.2:58404253-58404272 MS.gene44826:CDS 40.0%
CTTCTCTTATCTGCAAGCTT+CGG + chr5.2:58405122-58405141 None:intergenic 40.0%
CTTTCCAGATACTCCACAAA+AGG + chr5.2:58404918-58404937 None:intergenic 40.0%
GCTCAATTCTGTGCTTGATT+TGG - chr5.2:58405145-58405164 MS.gene44826:CDS 40.0%
GTGTTGGGATTCAAACTTGA+GGG - chr5.2:58404774-58404793 MS.gene44826:CDS 40.0%
TATGAGTTCTTGCGAGGAAT+CGG - chr5.2:58404414-58404433 MS.gene44826:CDS 40.0%
TCAAGACCATCAAGTTCATC+AGG + chr5.2:58404447-58404466 None:intergenic 40.0%
TCCTAACTCCCAATTTACCA+AGG + chr5.2:58404608-58404627 None:intergenic 40.0%
TGGCGAAATTCAAAGGAAAC+GGG - chr5.2:58404314-58404333 MS.gene44826:CDS 40.0%
TTACCAAGGTAATCAAGCAC+AGG + chr5.2:58404594-58404613 None:intergenic 40.0%
TTAGCAACCATGTTATGCCT+AGG + chr5.2:58405446-58405465 None:intergenic 40.0%
TTCAATGTTCTGTTGCTGGT+AGG - chr5.2:58404238-58404257 MS.gene44826:CDS 40.0%
TTCCCAGCATTCTTCACTTA+TGG - chr5.2:58405401-58405420 MS.gene44826:CDS 40.0%
! AACTAGATTCCCGGAGATTT+TGG - chr5.2:58404866-58404885 MS.gene44826:CDS 40.0%
! ACACTAGAATGAAGCACTTG+TGG + chr5.2:58404663-58404682 None:intergenic 40.0%
! ACTAGATTCCCGGAGATTTT+GGG - chr5.2:58404867-58404886 MS.gene44826:CDS 40.0%
! AGTGTTGGGATTCAAACTTG+AGG - chr5.2:58404773-58404792 MS.gene44826:CDS 40.0%
! GAAAGGATTGAAGTGTTCTC+TGG - chr5.2:58405466-58405485 MS.gene44826:CDS 40.0%
! GGTTTTCTGTGGATCAAATG+AGG - chr5.2:58405267-58405286 MS.gene44826:CDS 40.0%
! TAAGGATTGTGGGTTTTCTG+TGG - chr5.2:58405256-58405275 MS.gene44826:CDS 40.0%
! TAGAGTCATCAAGCCTTTTG+TGG - chr5.2:58404902-58404921 MS.gene44826:CDS 40.0%
! TGGTCTTGAACTTCCTGTTA+CGG - chr5.2:58404458-58404477 MS.gene44826:CDS 40.0%
!! TGTGCTTGATTTGGATGTTG+AGG - chr5.2:58405154-58405173 MS.gene44826:CDS 40.0%
AAGGCCTTTCCATCTCACTT+TGG + chr5.2:58405364-58405383 None:intergenic 45.0%
AAGTGAGATGGAAAGGCCTT+TGG - chr5.2:58405364-58405383 MS.gene44826:CDS 45.0%
AATCATTGAGAAGATGCCGC+AGG - chr5.2:58405187-58405206 MS.gene44826:CDS 45.0%
AATTCAAAGGAAACGGGGAG+GGG - chr5.2:58404320-58404339 MS.gene44826:CDS 45.0%
ACACTACATCCCAGTACAAG+AGG + chr5.2:58404555-58404574 None:intergenic 45.0%
GAAATTCAAAGGAAACGGGG+AGG - chr5.2:58404318-58404337 MS.gene44826:CDS 45.0%
GAATCTGATTCATCGCTCCT+TGG + chr5.2:58405605-58405624 None:intergenic 45.0%
GCCGAACATTAAACTCTTCG+AGG + chr5.2:58405040-58405059 None:intergenic 45.0%
GCTCAGTATCCTGACATCAT+TGG - chr5.2:58405083-58405102 MS.gene44826:CDS 45.0%
GCTTCATTCTAGTGTGGTTG+TGG - chr5.2:58404668-58404687 MS.gene44826:CDS 45.0%
GGAAAGGCCTTTGGTAGATT+TGG - chr5.2:58405373-58405392 MS.gene44826:CDS 45.0%
GGCGAAATTCAAAGGAAACG+GGG - chr5.2:58404315-58404334 MS.gene44826:CDS 45.0%
GTTCCTGTGCTTGATTACCT+TGG - chr5.2:58404588-58404607 MS.gene44826:CDS 45.0%
TCAGCATAGGTGTAGTACTG+AGG + chr5.2:58405220-58405239 None:intergenic 45.0%
TCAGGGGATGGATATCAAAC+CGG - chr5.2:58404716-58404735 MS.gene44826:CDS 45.0%
TGAATTCATTGATGCGGCCA+AGG - chr5.2:58405585-58405604 MS.gene44826:CDS 45.0%
TGGAAAGCTTGGGTATTCCA+AGG - chr5.2:58404931-58404950 MS.gene44826:CDS 45.0%
! AGTCTAGCAATAGCTAGCCT+TGG + chr5.2:58404951-58404970 None:intergenic 45.0%
! CTAGATTCCCGGAGATTTTG+GGG - chr5.2:58404868-58404887 MS.gene44826:CDS 45.0%
! GATTGAAGTGTTCTCTGGCA+TGG - chr5.2:58405471-58405490 MS.gene44826:CDS 45.0%
! GGTGTTGGAAGAAGAGAACT+CGG - chr5.2:58404834-58404853 MS.gene44826:CDS 45.0%
! GTGGAGTATCTGGAAAGCTT+GGG - chr5.2:58404921-58404940 MS.gene44826:CDS 45.0%
! TGTGGAGTATCTGGAAAGCT+TGG - chr5.2:58404920-58404939 MS.gene44826:CDS 45.0%
!! CAAGCCTTTTGTGGAGTATC+TGG - chr5.2:58404911-58404930 MS.gene44826:CDS 45.0%
!! GAGAGGTATCCTGAAGTGTT+GGG - chr5.2:58404759-58404778 MS.gene44826:CDS 45.0%
!! TGAGAGGTATCCTGAAGTGT+TGG - chr5.2:58404758-58404777 MS.gene44826:CDS 45.0%
AATTTCGCCAGAGTCCCGTT+TGG + chr5.2:58404304-58404323 None:intergenic 50.0%
CCACGTTAACGCAGTTCTTG+CGG - chr5.2:58404631-58404650 MS.gene44826:CDS 50.0%
CCCTGAAGATACTTGACCAC+CGG + chr5.2:58404702-58404721 None:intergenic 50.0%
CCGGTGGTCAAGTATCTTCA+GGG - chr5.2:58404699-58404718 MS.gene44826:CDS 50.0%
CGGTGGTCAAGTATCTTCAG+GGG - chr5.2:58404700-58404719 MS.gene44826:CDS 50.0%
GGATACCTCTCAAGAACACG+AGG + chr5.2:58404750-58404769 None:intergenic 50.0%
GGGACTCTGGCGAAATTCAA+AGG - chr5.2:58404307-58404326 MS.gene44826:CDS 50.0%
GGTCAAGTATCTTCAGGGGA+TGG - chr5.2:58404704-58404723 MS.gene44826:CDS 50.0%
GTACTGAGGCTAACTACCTG+CGG + chr5.2:58405206-58405225 None:intergenic 50.0%
GTTGGAAGAAGAGAACTCGG+AGG - chr5.2:58404837-58404856 MS.gene44826:CDS 50.0%
TATCTGCAAGCTTCGGCTCT+AGG + chr5.2:58405115-58405134 None:intergenic 50.0%
TGTGGTTGTGGATCTTGTGC+CGG - chr5.2:58404680-58404699 MS.gene44826:CDS 50.0%
! AGGTCGGTTCCAATGATGTC+AGG + chr5.2:58405095-58405114 None:intergenic 50.0%
ACCCGCATCCCCAAAATCTC+CGG + chr5.2:58404879-58404898 None:intergenic 55.0%
CCGCAAGAACTGCGTTAACG+TGG + chr5.2:58404634-58404653 None:intergenic 55.0%
GCCGGTGGTCAAGTATCTTC+AGG - chr5.2:58404698-58404717 MS.gene44826:CDS 55.0%
TGATTCGCGGGGATCCAAAC+GGG - chr5.2:58404287-58404306 MS.gene44826:CDS 55.0%
! CTGATTCGCGGGGATCCAAA+CGG - chr5.2:58404286-58404305 MS.gene44826:CDS 55.0%
!! GAGATTTTGGGGATGCGGGT+TGG - chr5.2:58404879-58404898 MS.gene44826:CDS 55.0%
!! TCCCGGAGATTTTGGGGATG+CGG - chr5.2:58404874-58404893 MS.gene44826:CDS 55.0%
!! TGCAAGCTTCGGCTCTAGGT+CGG + chr5.2:58405111-58405130 None:intergenic 55.0%
CCCGCATCCCCAAAATCTCC+GGG + chr5.2:58404878-58404897 None:intergenic 60.0%
GGTTGTGGATCTTGTGCCGG+TGG - chr5.2:58404683-58404702 MS.gene44826:CDS 60.0%
!! CCCGGAGATTTTGGGGATGC+GGG - chr5.2:58404875-58404894 MS.gene44826:CDS 60.0%
CGGGGATCCAAACGGGACTC+TGG - chr5.2:58404294-58404313 MS.gene44826:CDS 65.0%
TCCCCGCGAATCAGCAGCAC+CGG + chr5.2:58404280-58404299 None:intergenic 65.0%
!! ACCGGTGCTGCTGATTCGCG+GGG - chr5.2:58404276-58404295 MS.gene44826:CDS 65.0%
!! GACCGGTGCTGCTGATTCGC+GGG - chr5.2:58404275-58404294 MS.gene44826:CDS 65.0%
!! GGACCGGTGCTGCTGATTCG+CGG - chr5.2:58404274-58404293 MS.gene44826:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 58404138 58405679 58404138 ID=MS.gene44826
chr5.2 mRNA 58404138 58405679 58404138 ID=MS.gene44826.t1;Parent=MS.gene44826
chr5.2 exon 58404138 58405679 58404138 ID=MS.gene44826.t1.exon1;Parent=MS.gene44826.t1
chr5.2 CDS 58404138 58405679 58404138 ID=cds.MS.gene44826.t1;Parent=MS.gene44826.t1
Gene Sequence

>MS.gene44826

ATGCCAATTCTAACATTTGGTCATTCTAAGTTAACTGCTGCTACACTAGCTCTTCAATTGCCATGTAATTGTATTAGGAAGATAAGGTTTATTACAAAGATTCAATGTTCTGTTGCTGGTAGGACGTATAGTTATAGGACCGGTGCTGCTGATTCGCGGGGATCCAAACGGGACTCTGGCGAAATTCAAAGGAAACGGGGAGGGGCATCGTCGTTGTATGCCCGTCCGAGTTTATCTGAAATTAAGAAAGATAAGGCCACGTTGCGTGAAATTGTTTATGAGTTCTTGCGAGGAATCGGCATTGTTCCTGATGAACTTGATGGTCTTGAACTTCCTGTTACGGTTGATGTTATGAAGGAGCGTGTTGATTTTCTTCACAGTTTGGGGCTTACGATTGAAGACATTAACAACTATCCTCTTGTACTGGGATGTAGTGTCAAGAAGAATATGGTTCCTGTGCTTGATTACCTTGGTAAATTGGGAGTTAGGAAATCCACGTTAACGCAGTTCTTGCGGAGATATCCACAAGTGCTTCATTCTAGTGTGGTTGTGGATCTTGTGCCGGTGGTCAAGTATCTTCAGGGGATGGATATCAAACCGGATGATATTCCTCGTGTTCTTGAGAGGTATCCTGAAGTGTTGGGATTCAAACTTGAGGGAACCATGAGTACATCTGTTGCTTATTTGATTGGAATCGGTGTTGGAAGAAGAGAACTCGGAGGCATCTTAACTAGATTCCCGGAGATTTTGGGGATGCGGGTTGGTAGAGTCATCAAGCCTTTTGTGGAGTATCTGGAAAGCTTGGGTATTCCAAGGCTAGCTATTGCTAGACTGATAGAGACACAACCTTATATTCTTGGATTCGATTTGGACGAGAAAGTGAAGTCAAATGTCAAATCCCTCGAAGAGTTTAATGTTCGGCAGACATTGCTTGCCTCTATAATTGCTCAGTATCCTGACATCATTGGAACCGACCTAGAGCCGAAGCTTGCAGATAAGAGAAGTGTGCTCAATTCTGTGCTTGATTTGGATGTTGAGGATTTCGGCTTAATCATTGAGAAGATGCCGCAGGTAGTTAGCCTCAGTACTACACCTATGCTGAAGCACGTTGATTTTCTTAAGGATTGTGGGTTTTCTGTGGATCAAATGAGGAAGATGATTGTTGGATGCCCTCAACTACTTGCTTTAAACATTGATATCATGAAACTTAGCTTCGATTACTTCCAAAGTGAGATGGAAAGGCCTTTGGTAGATTTGGTTGAGTTCCCAGCATTCTTCACTTATGGTTTGGAGTCAACTATAAAACCTAGGCATAACATGGTTGCTAAGAAAGGATTGAAGTGTTCTCTGGCATGGATGCTTAATTGTTCTGATGAGAAATTTGAGCAACGAATGGACTATGACACTATTGATATGGAAGAGATGGAAATGGAACCATCATTTGACATGAATTCATTGATGCGGCCAAGGAGCGATGAATCAGATTCTGATTATGAAGACAGTGATTATGATGATGATGATGATGATGATGATGATGAGTAA
Protein sequence

>MS.gene44826.t1

MPILTFGHSKLTAATLALQLPCNCIRKIRFITKIQCSVAGRTYSYRTGAADSRGSKRDSGEIQRKRGGASSLYARPSLSEIKKDKATLREIVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLHSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTLTQFLRRYPQVLHSSVVVDLVPVVKYLQGMDIKPDDIPRVLERYPEVLGFKLEGTMSTSVAYLIGIGVGRRELGGILTRFPEILGMRVGRVIKPFVEYLESLGIPRLAIARLIETQPYILGFDLDEKVKSNVKSLEEFNVRQTLLASIIAQYPDIIGTDLEPKLADKRSVLNSVLDLDVEDFGLIIEKMPQVVSLSTTPMLKHVDFLKDCGFSVDQMRKMIVGCPQLLALNIDIMKLSFDYFQSEMERPLVDLVEFPAFFTYGLESTIKPRHNMVAKKGLKCSLAWMLNCSDEKFEQRMDYDTIDMEEMEMEPSFDMNSLMRPRSDESDSDYEDSDYDDDDDDDDDE