AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44859

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44859.t1 MTR_5g069710 96.091 307 12 0 1 307 1 307 0.0 617
MS.gene44859.t1 MTR_3g462790 66.565 329 81 9 1 307 1 322 8.09e-150 423
MS.gene44859.t1 MTR_7g067080 59.295 312 112 8 1 303 7 312 2.53e-118 343
MS.gene44859.t1 MTR_5g081860 61.881 202 70 3 4 203 2 198 2.02e-85 258
MS.gene44859.t1 MTR_1g111830 50.282 177 79 2 27 200 22 192 2.49e-56 182
MS.gene44859.t1 MTR_4g111975 44.118 238 95 5 29 233 8 240 5.03e-55 181
MS.gene44859.t1 MTR_8g063600 44.076 211 108 4 22 230 3 205 5.31e-50 167
MS.gene44859.t1 MTR_8g063870 36.471 255 120 8 23 270 2 221 5.37e-42 146
MS.gene44859.t1 MTR_4g100630 48.322 149 53 4 90 235 57 184 9.12e-32 120
MS.gene44859.t1 MTR_2g100930 73.239 71 19 0 127 197 104 174 1.48e-31 120
MS.gene44859.t1 MTR_6g092540 52.632 114 49 1 126 239 91 199 3.53e-31 118
MS.gene44859.t1 MTR_8g101650 69.333 75 22 1 123 197 96 169 2.98e-30 115
MS.gene44859.t1 MTR_4g015130 63.529 85 21 1 142 226 3 77 3.98e-30 109
MS.gene44859.t1 MTR_5g037080 70.513 78 23 0 126 203 96 173 1.33e-28 112
MS.gene44859.t1 MTR_4g107230 66.667 81 27 0 122 202 74 154 9.06e-27 106
MS.gene44859.t1 MTR_3g104370 47.967 123 50 4 129 240 88 207 3.39e-25 101
MS.gene44859.t1 MTR_5g088010 53.846 91 38 2 124 211 52 141 6.04e-25 100
MS.gene44859.t1 MTR_0036s0260 64.179 67 24 0 132 198 28 94 2.03e-23 95.9
MS.gene44859.t1 MTR_1g033620 83.019 53 9 0 148 200 21 73 4.14e-23 95.9
MS.gene44859.t1 MTR_5g088060 59.259 81 29 2 134 211 274 353 4.45e-23 99.0
MS.gene44859.t1 MTR_5g488160 59.259 81 29 2 134 211 274 353 4.45e-23 99.0
MS.gene44859.t1 MTR_2g090305 64.179 67 24 0 132 198 28 94 9.65e-23 93.6
MS.gene44859.t1 MTR_5g027570 55.814 86 35 2 128 211 88 172 1.46e-22 94.4
MS.gene44859.t1 MTR_5g075760 35.882 170 89 7 23 188 7 160 6.84e-22 92.0
MS.gene44859.t1 MTR_5g075790 35.088 171 88 6 23 188 16 168 2.62e-21 90.5
MS.gene44859.t1 MTR_1g033600 35.338 133 69 2 26 146 9 136 1.45e-20 86.3
MS.gene44859.t1 MTR_5g027550 60.526 76 27 1 128 200 73 148 3.28e-20 87.8
MS.gene44859.t1 MTR_1g022290 49.296 71 36 0 26 96 4 74 1.54e-19 82.0
MS.gene44859.t1 MTR_5g020170 44.000 75 42 0 17 91 7 81 2.88e-19 81.6
MS.gene44859.t1 MTR_3g116720 52.308 65 31 0 29 93 16 80 7.16e-19 79.7
MS.gene44859.t1 MTR_8g077360 51.389 72 34 1 25 96 1 71 7.18e-19 79.7
MS.gene44859.t1 MTR_8g077380 51.389 72 34 1 25 96 1 71 7.21e-19 79.7
MS.gene44859.t1 MTR_8g077390 51.389 72 34 1 25 96 1 71 7.21e-19 79.7
MS.gene44859.t1 MTR_1g048660 56.250 64 28 0 133 196 13 76 8.59e-19 80.1
MS.gene44859.t1 MTR_3g111880 54.545 66 30 0 26 91 4 69 1.49e-18 79.3
MS.gene44859.t1 MTR_6g004250 47.222 72 38 0 26 97 7 78 1.79e-17 76.3
MS.gene44859.t1 MTR_8g077420 49.231 65 32 1 25 89 1 64 1.06e-15 70.9
MS.gene44859.t1 MTR_3g013440 45.349 86 35 2 26 110 7 81 1.65e-15 71.2
MS.gene44859.t1 MTR_5g020540 50.820 61 29 1 29 89 4 63 7.37e-15 68.6
MS.gene44859.t1 MTR_3g070110 37.984 129 67 2 80 201 49 171 1.39e-14 72.0
MS.gene44859.t1 MTR_7g089210 53.333 60 28 0 32 91 15 74 3.14e-14 67.4
MS.gene44859.t1 MTR_1g083180 52.055 73 30 2 134 202 63 134 2.65e-13 67.8
MS.gene44859.t1 MTR_3g111920 44.444 63 35 0 29 91 3 65 3.40e-12 61.6
MS.gene44859.t1 MTR_6g043480 46.774 62 32 1 117 178 93 153 9.56e-12 63.9
MS.gene44859.t1 MTR_8g072990 43.056 72 36 2 129 196 97 167 2.08e-11 63.2
MS.gene44859.t1 MTR_6g043490 53.191 47 22 0 132 178 107 153 2.38e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44859.t1 AT2G38090 66.346 208 57 2 5 201 5 210 2.17e-99 294
MS.gene44859.t1 AT5G58900 68.224 214 53 4 5 204 1 213 8.25e-95 282
MS.gene44859.t1 AT5G01200 61.500 200 62 5 16 201 17 215 4.10e-78 239
MS.gene44859.t1 AT3G11280 67.052 173 48 2 29 201 33 196 8.06e-78 238
MS.gene44859.t1 AT3G11280 67.052 173 48 2 29 201 33 196 8.06e-78 238
MS.gene44859.t1 AT5G05790 63.021 192 63 4 14 201 13 200 8.78e-78 238
MS.gene44859.t1 AT5G05790 63.021 192 63 4 14 201 13 200 8.78e-78 238
MS.gene44859.t1 AT1G49010 50.739 203 73 5 23 200 2 202 2.60e-54 179
MS.gene44859.t1 AT5G08520 51.136 176 81 3 29 200 12 186 5.59e-54 178
MS.gene44859.t1 AT5G04760 41.441 222 117 3 26 244 3 214 1.07e-49 164
MS.gene44859.t1 AT5G23650 41.026 195 89 3 18 197 2 185 1.91e-41 147
MS.gene44859.t1 AT5G47390 60.185 108 35 3 92 197 60 161 1.14e-31 121
MS.gene44859.t1 AT3G10580 41.573 178 80 7 23 197 2 158 2.63e-31 118
MS.gene44859.t1 AT3G10580 42.442 172 75 7 29 197 8 158 4.90e-31 118
MS.gene44859.t1 AT4G09450 36.744 215 110 5 28 242 6 194 8.62e-31 115
MS.gene44859.t1 AT5G61620 48.819 127 63 1 127 253 103 227 5.83e-29 113
MS.gene44859.t1 AT5G56840 50.000 104 51 1 98 200 57 160 3.26e-27 106
MS.gene44859.t1 AT1G70000 70.833 72 21 0 126 197 92 163 1.21e-26 105
MS.gene44859.t1 AT1G70000 70.833 72 21 0 126 197 92 163 1.21e-26 105
MS.gene44859.t1 AT3G16350 60.870 92 36 0 127 218 132 223 1.89e-25 105
MS.gene44859.t1 AT1G74840 59.794 97 35 2 127 220 91 186 5.93e-25 100
MS.gene44859.t1 AT1G74840 59.794 97 35 2 127 220 91 186 8.20e-25 100
MS.gene44859.t1 AT1G19000 52.727 110 48 1 128 237 98 203 1.57e-24 100
MS.gene44859.t1 AT1G19000 52.727 110 48 1 128 237 98 203 1.57e-24 100
MS.gene44859.t1 AT1G75250 58.462 65 27 0 29 93 12 76 1.96e-21 88.2
MS.gene44859.t1 AT1G75250 58.462 65 27 0 29 93 12 76 2.59e-21 87.0
MS.gene44859.t1 AT3G10590 35.912 181 98 6 26 197 4 175 5.98e-21 89.4
MS.gene44859.t1 AT2G21650 52.941 68 32 0 29 96 14 81 8.61e-18 77.8
MS.gene44859.t1 AT4G39250 49.231 65 33 0 29 93 14 78 1.52e-16 74.3
MS.gene44859.t1 AT1G19510 51.613 62 30 0 32 93 15 76 2.82e-15 70.9
MS.gene44859.t1 AT2G18328 52.542 59 28 0 33 91 15 73 4.76e-13 63.9
MS.gene44859.t1 AT4G36570 56.818 44 19 0 29 72 12 55 1.02e-11 59.7

Find 61 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTTTGCCATACTTCTTT+AGG 0.307378 5.2:-58876696 None:intergenic
TATTTCATAAGACAACTTTC+AGG 0.314437 5.2:+58876790 MS.gene44859:CDS
TTAGATTGGGTGAACAGTTC+TGG 0.315891 5.2:+58876016 MS.gene44859:CDS
CCTTTCTTCCTTTCCTGCTC+AGG 0.322349 5.2:-58876088 None:intergenic
CTTCACCCTTTACCTTAGAT+TGG 0.332398 5.2:+58876002 MS.gene44859:CDS
CTACTAGGCCTAAAGAAGTA+TGG 0.375050 5.2:+58876688 MS.gene44859:CDS
GCTGGGTTGATTCCAGTTCC+TGG 0.382171 5.2:+58875965 MS.gene44859:CDS
TGGTGAAGTGGATCTATTGC+TGG 0.387915 5.2:-58876899 None:intergenic
ACATCACCGACCGTCTTTCC+TGG 0.388704 5.2:-58875896 None:intergenic
AGAACTGTTCACCCAATCTA+AGG 0.390954 5.2:-58876014 None:intergenic
GATGTTTGTAATATAGAAGC+TGG 0.393501 5.2:+58875947 MS.gene44859:CDS
AACATTATTCACATGCATAA+AGG 0.404368 5.2:+58877166 MS.gene44859:CDS
TGATAAGATGAAGTGGGAAA+TGG 0.405855 5.2:+58875712 None:intergenic
AAGTTTGTTGGTGCTGCTGC+TGG 0.410002 5.2:-58876945 None:intergenic
TGATGTGATGAGACAATATA+AGG 0.419767 5.2:+58875913 MS.gene44859:CDS
AGAAGTATGGCAAAGGTGAT+TGG 0.420618 5.2:+58876701 MS.gene44859:CDS
TTCACCCTTTACCTTAGATT+GGG 0.424588 5.2:+58876003 MS.gene44859:CDS
GTTGTGATGAACGTTGCATT+TGG 0.427349 5.2:-58877146 None:intergenic
CAAGACCAAAATCTTCAAAA+AGG 0.427744 5.2:+58877084 MS.gene44859:CDS
GTTATCTTCCATACACCAAT+TGG 0.440080 5.2:-58875768 None:intergenic
TGCTCATGAGCTGGATTGAA+AGG 0.456365 5.2:-58877015 None:intergenic
TTGTGTTCCTCTTCAGTCCA+TGG 0.459712 5.2:-58876115 None:intergenic
CATACACACAAAACTCCAAT+TGG 0.463612 5.2:+58875753 MS.gene44859:CDS
TACGATGAATTCAGAGGAAG+TGG 0.471644 5.2:+58876040 MS.gene44859:CDS
GCATGGCTTGCTACTTGAGT+TGG 0.471843 5.2:-58876760 None:intergenic
GGAGTGTTATAACCAGGAAC+TGG 0.478409 5.2:-58875977 None:intergenic
TTCACCCAATCTAAGGTAAA+GGG 0.481797 5.2:-58876007 None:intergenic
ACAAAACTCCAATTGGTGTA+TGG 0.484625 5.2:+58875760 MS.gene44859:CDS
GTTCACCCAATCTAAGGTAA+AGG 0.487408 5.2:-58876008 None:intergenic
TCCTGCAGATTATTTCTACT+AGG 0.501666 5.2:+58876673 MS.gene44859:intron
ATGAAAACTTGCTCATGAGC+TGG 0.506678 5.2:-58877024 None:intergenic
TCTAAGGTAAAGGGTGAAGT+AGG 0.513528 5.2:-58875998 None:intergenic
TTAGAGCTCCTGAGCAGGAA+AGG 0.515723 5.2:+58876080 MS.gene44859:CDS
CCTGAGCAGGAAAGGAAGAA+AGG 0.521387 5.2:+58876088 MS.gene44859:CDS
TTTAGTTAGAGCTCCTGAGC+AGG 0.523673 5.2:+58876075 MS.gene44859:CDS
GAAAACTTGATAAGATGAAG+TGG 0.527680 5.2:+58875705 None:intergenic
CATAGAATTCTGTGGTGAAG+TGG 0.532652 5.2:-58876911 None:intergenic
GCTACTTTATGCCATCGATC+CGG 0.541949 5.2:-58875863 None:intergenic
ATAAAGATACGCCGGATCGA+TGG 0.544701 5.2:+58875852 MS.gene44859:CDS
ATTTGGAAAACCATGTTATG+TGG 0.549103 5.2:-58877129 None:intergenic
TTGTTGCAGTCGAAGTTTGT+TGG 0.563497 5.2:-58876957 None:intergenic
CTCTTCTGGAGTCCATTGTG+TGG 0.588727 5.2:-58875795 None:intergenic
AAAGTAGCTGAAATGATACC+AGG 0.595280 5.2:+58875878 MS.gene44859:CDS
TTCATAAGACAACTTTCAGG+AGG 0.595999 5.2:+58876793 MS.gene44859:CDS
GTCTTCTTATCCACATAACA+TGG 0.600765 5.2:+58877119 MS.gene44859:CDS
ATGTTTGTAATATAGAAGCT+GGG 0.604659 5.2:+58875948 MS.gene44859:CDS
AGGAGTGCCATGGACTGAAG+AGG 0.610163 5.2:+58876108 MS.gene44859:CDS
AAAACTTGATAAGATGAAGT+GGG 0.612600 5.2:+58875706 None:intergenic
GGCCTAAAGAAGTATGGCAA+AGG 0.616426 5.2:+58876694 MS.gene44859:CDS
TTTGTGTGTATGATGCAGGA+GGG 0.617317 5.2:-58875742 None:intergenic
AAAGGAAGAAAGGAGTGCCA+TGG 0.621552 5.2:+58876098 MS.gene44859:CDS
TGAAATGATACCAGGAAAGA+CGG 0.622184 5.2:+58875886 MS.gene44859:CDS
TTTCAGGAGGAAAAGACAAG+AGG 0.628437 5.2:+58876806 MS.gene44859:CDS
ATGATACCAGGAAAGACGGT+CGG 0.635685 5.2:+58875890 MS.gene44859:CDS
TCTGGTTACGATGAATTCAG+AGG 0.640239 5.2:+58876034 MS.gene44859:CDS
GAAGTAGGAGTGTTATAACC+AGG 0.644855 5.2:-58875983 None:intergenic
AAGATAACATGACCACACAA+TGG 0.675526 5.2:+58875783 MS.gene44859:CDS
AGTTCATGATAAAGATACGC+CGG 0.676724 5.2:+58875844 MS.gene44859:CDS
TCATAGGAGTTAAAACCATG+AGG 0.695559 5.2:-58877051 None:intergenic
GAAAGTAACATAGAATTCTG+TGG 0.702812 5.2:-58876919 None:intergenic
AAGCATACATGACATAACAA+CGG 0.737256 5.2:+58876834 MS.gene44859:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTATAAATAAATTTAAA+GGG + chr5.2:58876414-58876433 MS.gene44859:intron 0.0%
!! TTATTTATAAATAAATTTAA+AGG + chr5.2:58876413-58876432 MS.gene44859:intron 0.0%
!! TATAAATAAATTTAAAGGGT+TGG + chr5.2:58876418-58876437 MS.gene44859:intron 15.0%
!!! TTAAGAAAGTATAGTAATAA+TGG + chr5.2:58876344-58876363 MS.gene44859:intron 15.0%
!! ATATCATATTAAGTTAGAGT+AGG + chr5.2:58876576-58876595 MS.gene44859:intron 20.0%
!!! TACAATAGTAATTTAGAACT+AGG + chr5.2:58876375-58876394 MS.gene44859:intron 20.0%
!!! TAGTATTTTTTCCTTTACTA+GGG + chr5.2:58876224-58876243 MS.gene44859:intron 20.0%
!!! TTAGTATTTTTTCCTTTACT+AGG + chr5.2:58876223-58876242 MS.gene44859:intron 20.0%
! ATGTTTGTAATATAGAAGCT+GGG + chr5.2:58875948-58875967 MS.gene44859:CDS 25.0%
! CTAACTTAATATGATATTCG+AGG - chr5.2:58876573-58876592 None:intergenic 25.0%
! GTTAATCTCTCAGAAAAAAT+TGG + chr5.2:58876856-58876875 MS.gene44859:CDS 25.0%
! TAACTTAAGATAGTAACAGA+AGG - chr5.2:58876644-58876663 None:intergenic 25.0%
! TAGAATTTAAACCCTAGTAA+AGG - chr5.2:58876238-58876257 None:intergenic 25.0%
! TATTTCATAAGACAACTTTC+AGG + chr5.2:58876790-58876809 MS.gene44859:CDS 25.0%
!! AATAGTAATTTAGAACTAGG+AGG + chr5.2:58876378-58876397 MS.gene44859:intron 25.0%
!! GAAAGTATAGTAATAATGGA+TGG + chr5.2:58876348-58876367 MS.gene44859:intron 25.0%
!! TCAAATATTTTGTTCTCTTC+TGG - chr5.2:58875812-58875831 None:intergenic 25.0%
!! TTATGAAATACTTTTGAGCA+TGG - chr5.2:58876780-58876799 None:intergenic 25.0%
!!! ATGATATAAGTTGGTTTTGT+AGG + chr5.2:58876601-58876620 MS.gene44859:intron 25.0%
!!! GTTGGATATTTTCATTTTTC+AGG + chr5.2:58876436-58876455 MS.gene44859:intron 25.0%
!!! TCATTTTTCAGGTTGTAATA+AGG + chr5.2:58876447-58876466 MS.gene44859:intron 25.0%
!!! TGATATAAGTTGGTTTTGTA+GGG + chr5.2:58876602-58876621 MS.gene44859:intron 25.0%
AAGCATACATGACATAACAA+CGG + chr5.2:58876834-58876853 MS.gene44859:CDS 30.0%
ATTTGGAAAACCATGTTATG+TGG - chr5.2:58877132-58877151 None:intergenic 30.0%
CAAGACCAAAATCTTCAAAA+AGG + chr5.2:58877084-58877103 MS.gene44859:CDS 30.0%
GAAAGTAACATAGAATTCTG+TGG - chr5.2:58876922-58876941 None:intergenic 30.0%
GAGTAGGTTATGATATAAGT+TGG + chr5.2:58876592-58876611 MS.gene44859:intron 30.0%
GATGTTTGTAATATAGAAGC+TGG + chr5.2:58875947-58875966 MS.gene44859:CDS 30.0%
TGATGTGATGAGACAATATA+AGG + chr5.2:58875913-58875932 MS.gene44859:CDS 30.0%
!! CAAGACCTTTTTGAAGATTT+TGG - chr5.2:58877092-58877111 None:intergenic 30.0%
AAAGTAGCTGAAATGATACC+AGG + chr5.2:58875878-58875897 MS.gene44859:CDS 35.0%
AAGATAACATGACCACACAA+TGG + chr5.2:58875783-58875802 MS.gene44859:CDS 35.0%
ACAAAACTCCAATTGGTGTA+TGG + chr5.2:58875760-58875779 MS.gene44859:CDS 35.0%
ACCTTACAACTTGTAAGGTA+AGG - chr5.2:58876534-58876553 None:intergenic 35.0%
AGTTCATGATAAAGATACGC+CGG + chr5.2:58875844-58875863 MS.gene44859:CDS 35.0%
CATACACACAAAACTCCAAT+TGG + chr5.2:58875753-58875772 MS.gene44859:CDS 35.0%
GTCTTCTTATCCACATAACA+TGG + chr5.2:58877119-58877138 MS.gene44859:CDS 35.0%
GTTATCTTCCATACACCAAT+TGG - chr5.2:58875771-58875790 None:intergenic 35.0%
TCATAGGAGTTAAAACCATG+AGG - chr5.2:58877054-58877073 None:intergenic 35.0%
TCCTTACCTTACAAGTTGTA+AGG + chr5.2:58876530-58876549 MS.gene44859:intron 35.0%
TGAAATGATACCAGGAAAGA+CGG + chr5.2:58875886-58875905 MS.gene44859:CDS 35.0%
TTCACCCTTTACCTTAGATT+GGG + chr5.2:58876003-58876022 MS.gene44859:CDS 35.0%
TTCATAAGACAACTTTCAGG+AGG + chr5.2:58876793-58876812 MS.gene44859:CDS 35.0%
! GTAATTTAGAACTAGGAGGT+AGG + chr5.2:58876382-58876401 MS.gene44859:intron 35.0%
! TTCACCCAATCTAAGGTAAA+GGG - chr5.2:58876010-58876029 None:intergenic 35.0%
! TTTAGACTTATCACTAGCCT+AGG - chr5.2:58876487-58876506 None:intergenic 35.0%
!! GTCTTGCATTTTAACCTCAT+AGG - chr5.2:58877070-58877089 None:intergenic 35.0%
!! TCCTGCAGATTATTTCTACT+AGG + chr5.2:58876673-58876692 MS.gene44859:intron 35.0%
!!! AGGTTTTTTAGCTTTCACCT+AGG + chr5.2:58876467-58876486 MS.gene44859:intron 35.0%
!!! TCATGGTTTTAACTCCTATG+AGG + chr5.2:58877053-58877072 MS.gene44859:CDS 35.0%
ACAAGTTGTAAGGTCGAGTT+AGG + chr5.2:58876540-58876559 MS.gene44859:intron 40.0%
ACTCGACCTTACAACTTGTA+AGG - chr5.2:58876539-58876558 None:intergenic 40.0%
AGAACTGTTCACCCAATCTA+AGG - chr5.2:58876017-58876036 None:intergenic 40.0%
CACCTTTGCCATACTTCTTT+AGG - chr5.2:58876699-58876718 None:intergenic 40.0%
CATAGAATTCTGTGGTGAAG+TGG - chr5.2:58876914-58876933 None:intergenic 40.0%
CTACTAGGCCTAAAGAAGTA+TGG + chr5.2:58876688-58876707 MS.gene44859:CDS 40.0%
CTTCACCCTTTACCTTAGAT+TGG + chr5.2:58876002-58876021 MS.gene44859:CDS 40.0%
GCCTAGTAGAAATAATCTGC+AGG - chr5.2:58876677-58876696 None:intergenic 40.0%
GTTGTGATGAACGTTGCATT+TGG - chr5.2:58877149-58877168 None:intergenic 40.0%
TACGATGAATTCAGAGGAAG+TGG + chr5.2:58876040-58876059 MS.gene44859:CDS 40.0%
TCTGGTTACGATGAATTCAG+AGG + chr5.2:58876034-58876053 MS.gene44859:CDS 40.0%
TTAGATTGGGTGAACAGTTC+TGG + chr5.2:58876016-58876035 MS.gene44859:CDS 40.0%
TTGTTGCAGTCGAAGTTTGT+TGG - chr5.2:58876960-58876979 None:intergenic 40.0%
TTTCAGGAGGAAAAGACAAG+AGG + chr5.2:58876806-58876825 MS.gene44859:CDS 40.0%
TTTGTGTGTATGATGCAGGA+GGG - chr5.2:58875745-58875764 None:intergenic 40.0%
! AGAAGTATGGCAAAGGTGAT+TGG + chr5.2:58876701-58876720 MS.gene44859:CDS 40.0%
! AGCTCATGAGCAAGTTTTCA+TGG + chr5.2:58877026-58877045 MS.gene44859:CDS 40.0%
! ATGAAAACTTGCTCATGAGC+TGG - chr5.2:58877027-58877046 None:intergenic 40.0%
! CAAGTTTTCATGGATCCTCA+TGG + chr5.2:58877036-58877055 MS.gene44859:CDS 40.0%
! CGACTGCAACAAATTTTCGT+TGG + chr5.2:58876968-58876987 MS.gene44859:CDS 40.0%
! CTGCAACAAATTTTCGTTGG+AGG + chr5.2:58876971-58876990 MS.gene44859:CDS 40.0%
! GTTCACCCAATCTAAGGTAA+AGG - chr5.2:58876011-58876030 None:intergenic 40.0%
! TCTAAGGTAAAGGGTGAAGT+AGG - chr5.2:58876001-58876020 None:intergenic 40.0%
! TTTTGTGTGTATGATGCAGG+AGG - chr5.2:58875746-58875765 None:intergenic 40.0%
!! GAAGTAGGAGTGTTATAACC+AGG - chr5.2:58875986-58876005 None:intergenic 40.0%
!! GAGTTTTGTGTGTATGATGC+AGG - chr5.2:58875749-58875768 None:intergenic 40.0%
ACCAGATGAGCTTCTAGTGT+TGG + chr5.2:58876279-58876298 MS.gene44859:intron 45.0%
ATAAAGATACGCCGGATCGA+TGG + chr5.2:58875852-58875871 MS.gene44859:CDS 45.0%
ATGATACCAGGAAAGACGGT+CGG + chr5.2:58875890-58875909 MS.gene44859:CDS 45.0%
TCCAACACTAGAAGCTCATC+TGG - chr5.2:58876283-58876302 None:intergenic 45.0%
TGCTCATGAGCTGGATTGAA+AGG - chr5.2:58877018-58877037 None:intergenic 45.0%
TGGTGAAGTGGATCTATTGC+TGG - chr5.2:58876902-58876921 None:intergenic 45.0%
TTGTGTTCCTCTTCAGTCCA+TGG - chr5.2:58876118-58876137 None:intergenic 45.0%
TTTAGTTAGAGCTCCTGAGC+AGG + chr5.2:58876075-58876094 MS.gene44859:CDS 45.0%
! GCTACTTTATGCCATCGATC+CGG - chr5.2:58875866-58875885 None:intergenic 45.0%
! GGAGTGTTATAACCAGGAAC+TGG - chr5.2:58875980-58875999 None:intergenic 45.0%
! GGCCTAAAGAAGTATGGCAA+AGG + chr5.2:58876694-58876713 MS.gene44859:CDS 45.0%
! TGGTTTGCGCTACTCGTTAA+AGG - chr5.2:58876263-58876282 None:intergenic 45.0%
!! AAAGGAAGAAAGGAGTGCCA+TGG + chr5.2:58876098-58876117 MS.gene44859:CDS 45.0%
CCTGAGCAGGAAAGGAAGAA+AGG + chr5.2:58876088-58876107 MS.gene44859:CDS 50.0%
CCTTTCTTCCTTTCCTGCTC+AGG - chr5.2:58876091-58876110 None:intergenic 50.0%
TTAGAGCTCCTGAGCAGGAA+AGG + chr5.2:58876080-58876099 MS.gene44859:CDS 50.0%
!! AAGTTTGTTGGTGCTGCTGC+TGG - chr5.2:58876948-58876967 None:intergenic 50.0%
!! CTCTTCTGGAGTCCATTGTG+TGG - chr5.2:58875798-58875817 None:intergenic 50.0%
!! GCATGGCTTGCTACTTGAGT+TGG - chr5.2:58876763-58876782 None:intergenic 50.0%
ACATCACCGACCGTCTTTCC+TGG - chr5.2:58875899-58875918 None:intergenic 55.0%
!! AGGAGTGCCATGGACTGAAG+AGG + chr5.2:58876108-58876127 MS.gene44859:CDS 55.0%
!! GCTGGGTTGATTCCAGTTCC+TGG + chr5.2:58875965-58875984 MS.gene44859:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 58875719 58877185 58875719 ID=MS.gene44859
chr5.2 mRNA 58875719 58877185 58875719 ID=MS.gene44859.t1;Parent=MS.gene44859
chr5.2 exon 58875719 58876137 58875719 ID=MS.gene44859.t1.exon1;Parent=MS.gene44859.t1
chr5.2 CDS 58875719 58876137 58875719 ID=cds.MS.gene44859.t1;Parent=MS.gene44859.t1
chr5.2 exon 58876681 58877185 58876681 ID=MS.gene44859.t1.exon2;Parent=MS.gene44859.t1
chr5.2 CDS 58876681 58877185 58876681 ID=cds.MS.gene44859.t1;Parent=MS.gene44859.t1
Gene Sequence

>MS.gene44859

ATGAAGTGGGAAATGGAAGTTATCCCTCCTGCATCATACACACAAAACTCCAATTGGTGTATGGAAGATAACATGACCACACAATGGACTCCAGAAGAGAACAAAATATTTGAAAATGCTCTTGCAGTTCATGATAAAGATACGCCGGATCGATGGCATAAAGTAGCTGAAATGATACCAGGAAAGACGGTCGGTGATGTGATGAGACAATATAAGGAATTAGAAGATGATGTTTGTAATATAGAAGCTGGGTTGATTCCAGTTCCTGGTTATAACACTCCTACTTCACCCTTTACCTTAGATTGGGTGAACAGTTCTGGTTACGATGAATTCAGAGGAAGTGGAAAGAGATCTTCTTTAGTTAGAGCTCCTGAGCAGGAAAGGAAGAAAGGAGTGCCATGGACTGAAGAGGAACACAAGTAAATTTTTTACTTTTTGTATTTGTTTTGTTATTATTGTTGTTATTGTTTTAGAAAGAAAAATAATAGTATATTAGTTGTTATGTTAGTATTTTTTCCTTTACTAGGGTTTAAATTCTAAACCTTTAACGAGTAGCGCAAACCAGATGAGCTTCTAGTGTTGGATAGATATAGAAGAATGTATGTATGTTTGAAACATTGTGAGCTTAAGAAAGTATAGTAATAATGGATGGAATTTACAATAGTAATTTAGAACTAGGAGGTAGGAACAGCAATTATTTATAAATAAATTTAAAGGGTTGGATATTTTCATTTTTCAGGTTGTAATAAGGTTTTTTAGCTTTCACCTAGGCTAGTGATAAGTCTAAATAAAGCTTATAAAATTTAGACAATCCTTACCTTACAAGTTGTAAGGTCGAGTTAGGTCAAAATCCTCGAATATCATATTAAGTTAGAGTAGGTTATGATATAAGTTGGTTTTGTAGGGCTGATTTAGACTAAAACCTTCTGTTACTATCTTAAGTTAGAGTATATTTCCTGCAGATTATTTCTACTAGGCCTAAAGAAGTATGGCAAAGGTGATTGGAGAAATATTTCGCGCAATTTCGTCATCACAAGAACACCAACTCAAGTAGCAAGCCATGCTCAAAAGTATTTCATAAGACAACTTTCAGGAGGAAAAGACAAGAGGAGAGCAAGCATACATGACATAACAACGGTTAATCTCTCAGAAAAAATTGGAACTTGTTCTTCAGAAGACACCAGCAATAGATCCACTTCACCACAGAATTCTATGTTACTTTCACACCAGCAGCAGCACCAACAAACTTCGACTGCAACAAATTTTCGTTGGAGGAATGATCAACAAAATGCTATGCCTTTCAATCCAGCTCATGAGCAAGTTTTCATGGATCCTCATGGTTTTAACTCCTATGAGGTTAAAATGCAAGACCAAAATCTTCAAAAAGGTCTTGTTCATGAGTCTTCTTATCCACATAACATGGTTTTCCAAATGCAACGTTCATCACAACATTATTCACATGCATAA
Protein sequence

>MS.gene44859.t1

MKWEMEVIPPASYTQNSNWCMEDNMTTQWTPEENKIFENALAVHDKDTPDRWHKVAEMIPGKTVGDVMRQYKELEDDVCNIEAGLIPVPGYNTPTSPFTLDWVNSSGYDEFRGSGKRSSLVRAPEQERKKGVPWTEEEHKLFLLGLKKYGKGDWRNISRNFVITRTPTQVASHAQKYFIRQLSGGKDKRRASIHDITTVNLSEKIGTCSSEDTSNRSTSPQNSMLLSHQQQHQQTSTATNFRWRNDQQNAMPFNPAHEQVFMDPHGFNSYEVKMQDQNLQKGLVHESSYPHNMVFQMQRSSQHYSHA