AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45192

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45192.t1 MTR_5g037080 96.508 315 8 2 1 313 1 314 0.0 615
MS.gene45192.t1 MTR_4g100630 49.505 202 65 5 3 185 1 184 6.17e-56 184
MS.gene45192.t1 MTR_2g100930 49.268 205 60 6 3 180 1 188 2.82e-53 178
MS.gene45192.t1 MTR_4g107230 56.061 198 56 7 24 206 8 189 1.37e-48 164
MS.gene45192.t1 MTR_6g092540 47.126 174 69 4 3 165 1 162 1.71e-45 156
MS.gene45192.t1 MTR_8g101650 41.667 204 85 6 5 185 4 196 3.00e-40 142
MS.gene45192.t1 MTR_0036s0260 82.353 68 12 0 100 167 28 95 2.04e-34 125
MS.gene45192.t1 MTR_3g462790 68.966 87 25 1 89 173 134 220 5.85e-34 127
MS.gene45192.t1 MTR_2g090305 80.882 68 13 0 100 167 28 95 1.55e-33 122
MS.gene45192.t1 MTR_4g111975 72.603 73 20 0 94 166 132 204 3.75e-33 124
MS.gene45192.t1 MTR_5g069710 70.513 78 23 0 94 171 126 203 2.30e-32 122
MS.gene45192.t1 MTR_3g104370 43.114 167 83 5 1 164 1 158 5.22e-32 119
MS.gene45192.t1 MTR_7g067080 67.949 78 25 0 94 171 134 211 5.15e-31 119
MS.gene45192.t1 MTR_1g111830 68.421 76 24 0 91 166 115 190 1.05e-30 116
MS.gene45192.t1 MTR_5g081860 50.847 118 53 2 87 202 112 226 9.69e-30 114
MS.gene45192.t1 MTR_8g063600 60.759 79 29 1 89 165 95 173 4.65e-25 102
MS.gene45192.t1 MTR_4g015130 56.923 65 28 0 110 174 3 67 3.43e-20 83.6
MS.gene45192.t1 MTR_5g088010 40.385 104 56 2 94 191 54 157 3.75e-20 88.2
MS.gene45192.t1 MTR_1g048660 55.224 67 30 0 101 167 13 79 8.43e-20 82.8
MS.gene45192.t1 MTR_5g027570 50.617 81 34 2 93 167 81 161 1.34e-19 86.3
MS.gene45192.t1 MTR_8g063870 56.061 66 27 1 102 165 94 159 4.92e-19 85.5
MS.gene45192.t1 MTR_5g088060 43.750 96 48 2 102 191 274 369 1.05e-18 86.7
MS.gene45192.t1 MTR_5g488160 43.750 96 48 2 102 191 274 369 1.05e-18 86.7
MS.gene45192.t1 MTR_1g033620 72.549 51 14 0 116 166 21 71 2.10e-18 83.2
MS.gene45192.t1 MTR_5g027550 65.455 55 19 0 93 147 70 124 2.26e-18 82.8
MS.gene45192.t1 MTR_5g075760 45.455 77 39 2 92 166 97 172 8.60e-13 67.0
MS.gene45192.t1 MTR_1g083180 48.571 70 32 2 102 168 63 131 1.18e-12 65.9
MS.gene45192.t1 MTR_5g075790 49.254 67 31 2 102 166 115 180 4.56e-12 65.1
MS.gene45192.t1 MTR_3g070110 45.833 72 32 1 102 166 97 168 7.71e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45192.t1 AT1G70000 49.226 323 90 11 3 313 1 261 5.03e-74 228
MS.gene45192.t1 AT1G70000 49.226 323 90 11 3 313 1 261 5.03e-74 228
MS.gene45192.t1 AT5G47390 53.297 182 53 3 3 169 1 165 1.74e-56 187
MS.gene45192.t1 AT5G61620 46.927 179 82 5 1 171 6 179 3.44e-47 161
MS.gene45192.t1 AT3G16350 42.358 229 69 4 3 180 1 217 2.57e-44 155
MS.gene45192.t1 AT1G74840 53.927 191 55 5 20 194 15 188 1.36e-43 149
MS.gene45192.t1 AT1G74840 56.571 175 45 4 20 180 15 172 2.25e-43 150
MS.gene45192.t1 AT5G56840 48.295 176 78 6 3 174 1 167 3.47e-43 148
MS.gene45192.t1 AT1G19000 52.850 193 52 6 20 190 13 188 4.78e-43 149
MS.gene45192.t1 AT1G19000 52.850 193 52 6 20 190 13 188 4.78e-43 149
MS.gene45192.t1 AT5G58900 64.773 88 29 1 90 175 129 216 2.21e-33 124
MS.gene45192.t1 AT1G49010 73.973 73 19 0 94 166 128 200 3.77e-33 124
MS.gene45192.t1 AT2G38090 69.333 75 23 0 95 169 136 210 8.66e-32 120
MS.gene45192.t1 AT5G08520 69.863 73 22 0 94 166 112 184 9.41e-32 120
MS.gene45192.t1 AT5G04760 73.611 72 19 0 95 166 94 165 2.61e-31 117
MS.gene45192.t1 AT5G01200 67.123 73 24 0 94 166 140 212 1.43e-29 114
MS.gene45192.t1 AT3G11280 60.976 82 32 0 89 170 116 197 1.37e-28 111
MS.gene45192.t1 AT3G11280 60.976 82 32 0 89 170 116 197 1.37e-28 111
MS.gene45192.t1 AT5G05790 59.756 82 33 0 89 170 120 201 1.05e-27 109
MS.gene45192.t1 AT5G05790 59.756 82 33 0 89 170 120 201 1.05e-27 109
MS.gene45192.t1 AT5G23650 56.579 76 33 0 90 165 110 185 4.67e-22 95.1
MS.gene45192.t1 AT3G10580 57.143 70 28 1 96 165 91 158 1.37e-21 92.0
MS.gene45192.t1 AT4G09450 51.685 89 39 2 81 165 68 156 1.91e-21 90.5
MS.gene45192.t1 AT3G10580 57.143 70 28 1 96 165 91 158 2.02e-21 92.4
MS.gene45192.t1 AT3G10590 50.617 81 37 2 90 168 99 178 6.37e-19 84.0

Find 100 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATTGGTGTTGGCCTAAT+TGG 0.220605 5.1:-27619167 None:intergenic
ATCTCCGGCGGCGGCGGTTC+TGG 0.294270 5.1:-27618068 None:intergenic
CTTCTTGTTGGGGTTGCAAC+AGG 0.297790 5.1:+27617942 MS.gene45192:CDS
GACGAATGGCTGCTACTTTC+TGG 0.309990 5.1:-27619311 None:intergenic
CTGACCTCGTTTGCGTTCTC+TGG 0.320215 5.1:-27617808 None:intergenic
TGGTGGTGGTGGTGTTGGGG+TGG 0.320284 5.1:-27617653 None:intergenic
GCATATCCATCTTCACATTA+TGG 0.334586 5.1:+27619065 MS.gene45192:CDS
GAACACAAGCTCTTCTTGTT+GGG 0.336533 5.1:+27617931 MS.gene45192:CDS
GATGGAACCTTCAACTATAA+TGG 0.360343 5.1:+27618986 MS.gene45192:CDS
GTTGTTATTGGTGGTGGTGG+TGG 0.365994 5.1:-27617664 None:intergenic
AAAGAGTGGCCAAGCCAATT+AGG 0.366908 5.1:+27619153 MS.gene45192:CDS
CTCATTACCGGCAATGTTAC+CGG 0.380041 5.1:-27619125 None:intergenic
TCACATTATGGCGGCATCCC+TGG 0.390624 5.1:+27619077 MS.gene45192:CDS
GACTGGATCTCCGGCGGCGG+CGG 0.396452 5.1:-27618074 None:intergenic
ACTCTTTCAGCACTCATTAC+CGG 0.403021 5.1:-27619137 None:intergenic
AGGGTTGTTATTGGTGGTGG+TGG 0.429616 5.1:-27617667 None:intergenic
TCATCTTCCATTATAGTTGA+AGG 0.430302 5.1:-27618993 None:intergenic
TCTTGGTCCGGTAACATTGC+CGG 0.432456 5.1:+27619118 MS.gene45192:CDS
GCTTCCTCCTTCTTCTAAGA+TGG 0.434360 5.1:+27619187 MS.gene45192:CDS
GAAAGGGTTGTTATTGGTGG+TGG 0.436325 5.1:-27617670 None:intergenic
GTTGGGGTGGTTGTTTCCTC+CGG 0.442684 5.1:-27617640 None:intergenic
GTCGTCATCGGCTTCATCAT+CGG 0.450173 5.1:+27619331 MS.gene45192:CDS
CACATTATGGCGGCATCCCT+GGG 0.451781 5.1:+27619078 MS.gene45192:CDS
AACAGGTTGGTAAAGGTGAT+TGG 0.456244 5.1:+27617959 MS.gene45192:CDS
AGAACACAAGCTCTTCTTGT+TGG 0.458844 5.1:+27617930 MS.gene45192:CDS
TCTGAAAGGGTTGTTATTGG+TGG 0.460673 5.1:-27617673 None:intergenic
ATGGCTGCAGGGAAGTATAA+TGG 0.461919 5.1:+27619365 MS.gene45192:CDS
CCCATCGGAAAAGGTGTCCC+AGG 0.480895 5.1:-27619095 None:intergenic
GATGTCATCGGATACATAAC+CGG 0.484684 5.1:-27617766 None:intergenic
GATACATAACCGGCATCCGC+GGG 0.489451 5.1:-27617756 None:intergenic
TGGTTGGTGAAGGTGTTCCT+TGG 0.492029 5.1:+27617899 MS.gene45192:intron
TTGTTCATACTGAGAGAGAT+TGG 0.492243 5.1:-27617712 None:intergenic
GTCATTGAAGCTGCAAACTT+CGG 0.497600 5.1:+27619268 MS.gene45192:CDS
AGATTAGCCATCTTAGAAGA+AGG 0.500963 5.1:-27619194 None:intergenic
TTGTGTTCTTCCTCTGTCCA+AGG 0.501291 5.1:-27617916 None:intergenic
TGGTGTTGGCCTAATTGGCT+TGG 0.501496 5.1:-27619162 None:intergenic
TGACAATGGAAAAGGCTCAA+TGG 0.502601 5.1:-27619249 None:intergenic
CTAGAAACTTTGTGAAAACA+AGG 0.504909 5.1:+27617992 MS.gene45192:CDS
GATGACATCGTTCACGCCTC+TGG 0.506341 5.1:+27617780 MS.gene45192:CDS
AGCTTCAATGACAATGGAAA+AGG 0.506440 5.1:-27619257 None:intergenic
ATAACCGGCATCCGCGGGGT+TGG 0.510315 5.1:-27617751 None:intergenic
GGGTTGCAACAGGTTGGTAA+AGG 0.511124 5.1:+27617952 MS.gene45192:CDS
CGTTGCATGTTCTCGAGTTG+TGG 0.513462 5.1:-27617572 None:intergenic
TGTGAAGATGGATATGCGGC+GGG 0.518568 5.1:-27619059 None:intergenic
GGACCAAGACCCATCGGAAA+AGG 0.520312 5.1:-27619104 None:intergenic
TAGCAGCCATTCGTCGTCAT+CGG 0.522877 5.1:+27619319 MS.gene45192:CDS
AACACAAGCTCTTCTTGTTG+GGG 0.525017 5.1:+27617932 MS.gene45192:CDS
GGTGGTAATATCAAAGAGAC+TGG 0.526759 5.1:-27618091 None:intergenic
GATTCCAACCCCGCGGATGC+CGG 0.538280 5.1:+27617747 MS.gene45192:CDS
AGAGACTGGATCTCCGGCGG+CGG 0.543258 5.1:-27618077 None:intergenic
GCAGGGAAGTATAATGGTGG+GGG 0.547007 5.1:+27619371 MS.gene45192:CDS
ATAACCAGAACCGCCGCCGC+CGG 0.548822 5.1:+27618064 MS.gene45192:CDS
TATCAAAGAGACTGGATCTC+CGG 0.548838 5.1:-27618083 None:intergenic
TTAGAAGAAGGAGGAAGCAT+TGG 0.549090 5.1:-27619182 None:intergenic
CTTTGATATTACCACCGATA+CGG 0.550867 5.1:+27618098 MS.gene45192:CDS
GGGTTGGAATCTTGTGGAGG+TGG 0.557505 5.1:-27617735 None:intergenic
ATGCCGCCATAATGTGAAGA+TGG 0.557994 5.1:-27619071 None:intergenic
TTGTTGGGGTTGCAACAGGT+TGG 0.558381 5.1:+27617946 MS.gene45192:CDS
GCTGCAAACTTCGGATCCAT+CGG 0.562043 5.1:+27619277 MS.gene45192:CDS
ATTATACTTCCCTGCAGCCA+TGG 0.565515 5.1:-27619363 None:intergenic
AAGAACAAATATTACCGTAT+CGG 0.565553 5.1:-27618112 None:intergenic
TCTGGTGAATGATCTTCCGA+TGG 0.574513 5.1:-27619293 None:intergenic
TCCGCGGGGTTGGAATCTTG+TGG 0.576478 5.1:-27617741 None:intergenic
CTGCAGGGAAGTATAATGGT+GGG 0.579931 5.1:+27619369 MS.gene45192:CDS
TGCAGGGAAGTATAATGGTG+GGG 0.579949 5.1:+27619370 MS.gene45192:CDS
GTTTGCAGCTTCAATGACAA+TGG 0.579978 5.1:-27619263 None:intergenic
GGCGGCGGTTCTGGTTATGA+CGG 0.580334 5.1:-27618059 None:intergenic
GCATGACTTGCAACCTGAGT+TGG 0.584887 5.1:-27618018 None:intergenic
TGAAGATGGATATGCGGCGG+GGG 0.585757 5.1:-27619057 None:intergenic
GAAGGAGGAAGCATTGGTGT+TGG 0.586167 5.1:-27619176 None:intergenic
TGCTCACAGTGCGGCAACAA+CGG 0.587587 5.1:+27617549 MS.gene45192:CDS
AACAAATATTACCGTATCGG+TGG 0.590836 5.1:-27618109 None:intergenic
GAAAACAAGGACACCAACTC+AGG 0.592034 5.1:+27618005 MS.gene45192:CDS
GCGTTCTCTGGATCGACCAG+AGG 0.617091 5.1:-27617796 None:intergenic
ATGAAGCCGATGACGACGAA+TGG 0.619919 5.1:-27619325 None:intergenic
TGAAGGTGTTCCTTGGACAG+AGG 0.622183 5.1:+27617906 MS.gene45192:intron
TCGAGAACATGCAACGACGG+TGG 0.622712 5.1:+27617579 MS.gene45192:CDS
GGTTCTGGTTATGACGGCGG+AGG 0.625551 5.1:-27618053 None:intergenic
ATGTGAAGATGGATATGCGG+CGG 0.625832 5.1:-27619060 None:intergenic
GGATACATAACCGGCATCCG+CGG 0.626209 5.1:-27617757 None:intergenic
AGAGGCGTGAACGATGTCAT+CGG 0.629917 5.1:-27617778 None:intergenic
GTTACCGGACCAAGACCCAT+CGG 0.636658 5.1:-27619110 None:intergenic
TTTGGTGTTCGACTCACCGG+AGG 0.638984 5.1:+27617624 MS.gene45192:CDS
CCATCGGAAAAGGTGTCCCA+GGG 0.645708 5.1:-27619094 None:intergenic
AACTCGAGAACATGCAACGA+CGG 0.649079 5.1:+27617576 MS.gene45192:CDS
GCTGCAGGGAAGTATAATGG+TGG 0.654189 5.1:+27619368 MS.gene45192:CDS
GGTAATGAGTGCTGAAAGAG+TGG 0.656043 5.1:+27619139 MS.gene45192:CDS
CGATCCAGAGAACGCAAACG+AGG 0.663587 5.1:+27617804 MS.gene45192:CDS
GTTGGTAAAGGTGATTGGAG+AGG 0.666734 5.1:+27617964 MS.gene45192:CDS
TTAGCCATCTTAGAAGAAGG+AGG 0.666938 5.1:-27619191 None:intergenic
GCGGGGTTGGAATCTTGTGG+AGG 0.674293 5.1:-27617738 None:intergenic
GGCGGTTCTGGTTATGACGG+CGG 0.676927 5.1:-27618056 None:intergenic
CAAAGAGACTGGATCTCCGG+CGG 0.680570 5.1:-27618080 None:intergenic
TCTCGCACGTGCTCACAGTG+CGG 0.694033 5.1:+27617540 MS.gene45192:CDS
TATCCATCTTCACATTATGG+CGG 0.696782 5.1:+27619068 MS.gene45192:CDS
TCAATTTCAGCAAGAAACAG+TGG 0.701868 5.1:+27619016 MS.gene45192:CDS
ATAATGTGAAGATGGATATG+CGG 0.713042 5.1:-27619063 None:intergenic
GTGAAGATGGATATGCGGCG+GGG 0.716036 5.1:-27619058 None:intergenic
TCCACAAGATTCCAACCCCG+CGG 0.717102 5.1:+27617740 MS.gene45192:CDS
ATACATAACCGGCATCCGCG+GGG 0.725166 5.1:-27617755 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATACGAAATATATTTA+AGG - chr5.1:27618238-27618257 None:intergenic 10.0%
!! GAAATTGTTAAAAATATAAA+AGG - chr5.1:27618162-27618181 None:intergenic 10.0%
!!! TATATTAATGTTTTTTGTTT+TGG + chr5.1:27617879-27617898 MS.gene45192:intron 10.0%
!! TAAAATCAGATTATTAGAAA+AGG - chr5.1:27618184-27618203 None:intergenic 15.0%
!! TAATATATCATGATTACAAT+TGG - chr5.1:27618519-27618538 None:intergenic 15.0%
!!! TTAATGTTTTTTGTTTTGGT+TGG + chr5.1:27617883-27617902 MS.gene45192:intron 20.0%
! AAGAACAAATATTACCGTAT+CGG - chr5.1:27618115-27618134 None:intergenic 25.0%
! ATGAGCTTATGTTAATTGAT+AGG - chr5.1:27618777-27618796 None:intergenic 25.0%
! CACCTAAAATAAATAATCCA+AGG - chr5.1:27618967-27618986 None:intergenic 25.0%
! TATCAATTAACATAAGCTCA+TGG + chr5.1:27618776-27618795 MS.gene45192:intron 25.0%
!! CTATTTCGTTTTACTATAAG+AGG + chr5.1:27618741-27618760 MS.gene45192:intron 25.0%
!! TCATTAATGTAATTTTGCCT+TGG + chr5.1:27618947-27618966 MS.gene45192:intron 25.0%
!!! GAAAAAAGCATCATGATTTT+TGG + chr5.1:27617606-27617625 MS.gene45192:CDS 25.0%
!!! GGATTATTTATTTTAGGTGA+TGG + chr5.1:27618968-27618987 MS.gene45192:intron 25.0%
!!! TGCCTTGGATTATTTATTTT+AGG + chr5.1:27618962-27618981 MS.gene45192:intron 25.0%
ATAATGTGAAGATGGATATG+CGG - chr5.1:27619066-27619085 None:intergenic 30.0%
ATGTCCGGAGATAATATATT+TGG - chr5.1:27618836-27618855 None:intergenic 30.0%
CTAGAAACTTTGTGAAAACA+AGG + chr5.1:27617992-27618011 MS.gene45192:CDS 30.0%
TCATCTTCCATTATAGTTGA+AGG - chr5.1:27618996-27619015 None:intergenic 30.0%
TGTCCGGAGATAATATATTT+GGG - chr5.1:27618835-27618854 None:intergenic 30.0%
TGTTCGCAAAAATATATGTC+CGG - chr5.1:27618851-27618870 None:intergenic 30.0%
!! TTTTCTGAAAGGGTTGTTAT+TGG - chr5.1:27617679-27617698 None:intergenic 30.0%
!!! TTTTTTGTTTTGGTTGGTGA+AGG + chr5.1:27617889-27617908 MS.gene45192:intron 30.0%
AACAAATATTACCGTATCGG+TGG - chr5.1:27618112-27618131 None:intergenic 35.0%
AGCTTCAATGACAATGGAAA+AGG - chr5.1:27619260-27619279 None:intergenic 35.0%
CGGAGATAATATATTTGGGA+CGG - chr5.1:27618831-27618850 None:intergenic 35.0%
CGTCCCAAATATATTATCTC+CGG + chr5.1:27618829-27618848 MS.gene45192:intron 35.0%
CTTTGATATTACCACCGATA+CGG + chr5.1:27618098-27618117 MS.gene45192:CDS 35.0%
GATGGAACCTTCAACTATAA+TGG + chr5.1:27618986-27619005 MS.gene45192:CDS 35.0%
GCATATCCATCTTCACATTA+TGG + chr5.1:27619065-27619084 MS.gene45192:CDS 35.0%
TATCCATCTTCACATTATGG+CGG + chr5.1:27619068-27619087 MS.gene45192:CDS 35.0%
TCAATTTCAGCAAGAAACAG+TGG + chr5.1:27619016-27619035 MS.gene45192:CDS 35.0%
TTGTTCATACTGAGAGAGAT+TGG - chr5.1:27617715-27617734 None:intergenic 35.0%
! AGATAATATATTTGGGACGG+AGG - chr5.1:27618828-27618847 None:intergenic 35.0%
! AGATTAGCCATCTTAGAAGA+AGG - chr5.1:27619197-27619216 None:intergenic 35.0%
! GATAATATATTTGGGACGGA+GGG - chr5.1:27618827-27618846 None:intergenic 35.0%
!!! ATCATCGGCTTTTAAAACCA+TGG + chr5.1:27619346-27619365 MS.gene45192:CDS 35.0%
!!! ATGCTAGCACTTTTTCTGAA+AGG - chr5.1:27617690-27617709 None:intergenic 35.0%
!!! TGCTAGCACTTTTTCTGAAA+GGG - chr5.1:27617689-27617708 None:intergenic 35.0%
AACACAAGCTCTTCTTGTTG+GGG + chr5.1:27617932-27617951 MS.gene45192:CDS 40.0%
ACTCTTTCAGCACTCATTAC+CGG - chr5.1:27619140-27619159 None:intergenic 40.0%
AGAACACAAGCTCTTCTTGT+TGG + chr5.1:27617930-27617949 MS.gene45192:CDS 40.0%
GAACACAAGCTCTTCTTGTT+GGG + chr5.1:27617931-27617950 MS.gene45192:CDS 40.0%
GATGTCATCGGATACATAAC+CGG - chr5.1:27617769-27617788 None:intergenic 40.0%
GGTGGTAATATCAAAGAGAC+TGG - chr5.1:27618094-27618113 None:intergenic 40.0%
GTCATTGAAGCTGCAAACTT+CGG + chr5.1:27619268-27619287 MS.gene45192:CDS 40.0%
GTTTGCAGCTTCAATGACAA+TGG - chr5.1:27619266-27619285 None:intergenic 40.0%
TATCAAAGAGACTGGATCTC+CGG - chr5.1:27618086-27618105 None:intergenic 40.0%
TGACAATGGAAAAGGCTCAA+TGG - chr5.1:27619252-27619271 None:intergenic 40.0%
TTAGCCATCTTAGAAGAAGG+AGG - chr5.1:27619194-27619213 None:intergenic 40.0%
! TCTGAAAGGGTTGTTATTGG+TGG - chr5.1:27617676-27617695 None:intergenic 40.0%
! TTAGAAGAAGGAGGAAGCAT+TGG - chr5.1:27619185-27619204 None:intergenic 40.0%
!! AACAGGTTGGTAAAGGTGAT+TGG + chr5.1:27617959-27617978 MS.gene45192:CDS 40.0%
!!! ATTTTTGGTGTTCGACTCAC+CGG + chr5.1:27617621-27617640 MS.gene45192:CDS 40.0%
!!! CTTTTAAAACCATGGCTGCA+GGG + chr5.1:27619354-27619373 MS.gene45192:CDS 40.0%
AAAGAGTGGCCAAGCCAATT+AGG + chr5.1:27619153-27619172 MS.gene45192:CDS 45.0%
AACTCGAGAACATGCAACGA+CGG + chr5.1:27617576-27617595 MS.gene45192:CDS 45.0%
ATGCCGCCATAATGTGAAGA+TGG - chr5.1:27619074-27619093 None:intergenic 45.0%
ATGTGAAGATGGATATGCGG+CGG - chr5.1:27619063-27619082 None:intergenic 45.0%
ATTATACTTCCCTGCAGCCA+TGG - chr5.1:27619366-27619385 None:intergenic 45.0%
CTCATTACCGGCAATGTTAC+CGG - chr5.1:27619128-27619147 None:intergenic 45.0%
GAAAACAAGGACACCAACTC+AGG + chr5.1:27618005-27618024 MS.gene45192:CDS 45.0%
TCTGGTGAATGATCTTCCGA+TGG - chr5.1:27619296-27619315 None:intergenic 45.0%
TTGTGTTCTTCCTCTGTCCA+AGG - chr5.1:27617919-27617938 None:intergenic 45.0%
! ATGGCTGCAGGGAAGTATAA+TGG + chr5.1:27619365-27619384 MS.gene45192:CDS 45.0%
! CTGCAGGGAAGTATAATGGT+GGG + chr5.1:27619369-27619388 MS.gene45192:CDS 45.0%
! GAAAGGGTTGTTATTGGTGG+TGG - chr5.1:27617673-27617692 None:intergenic 45.0%
! GCTTCCTCCTTCTTCTAAGA+TGG + chr5.1:27619187-27619206 MS.gene45192:CDS 45.0%
! GTTGGTAAAGGTGATTGGAG+AGG + chr5.1:27617964-27617983 MS.gene45192:CDS 45.0%
! TGCAGGGAAGTATAATGGTG+GGG + chr5.1:27619370-27619389 MS.gene45192:CDS 45.0%
!! AGCATTGGTGTTGGCCTAAT+TGG - chr5.1:27619170-27619189 None:intergenic 45.0%
!! GGTAATGAGTGCTGAAAGAG+TGG + chr5.1:27619139-27619158 MS.gene45192:CDS 45.0%
!!! GCTTTTAAAACCATGGCTGC+AGG + chr5.1:27619353-27619372 MS.gene45192:CDS 45.0%
ATGAAGCCGATGACGACGAA+TGG - chr5.1:27619328-27619347 None:intergenic 50.0%
CTTCTTGTTGGGGTTGCAAC+AGG + chr5.1:27617942-27617961 MS.gene45192:CDS 50.0%
GCTGCAAACTTCGGATCCAT+CGG + chr5.1:27619277-27619296 MS.gene45192:CDS 50.0%
TAGCAGCCATTCGTCGTCAT+CGG + chr5.1:27619319-27619338 MS.gene45192:CDS 50.0%
TCTTGGTCCGGTAACATTGC+CGG + chr5.1:27619118-27619137 MS.gene45192:CDS 50.0%
TGTGAAGATGGATATGCGGC+GGG - chr5.1:27619062-27619081 None:intergenic 50.0%
TTGTTGGGGTTGCAACAGGT+TGG + chr5.1:27617946-27617965 MS.gene45192:CDS 50.0%
! ACACCTTTTCCGATGGGTCT+TGG + chr5.1:27619101-27619120 MS.gene45192:CDS 50.0%
! AGAGGCGTGAACGATGTCAT+CGG - chr5.1:27617781-27617800 None:intergenic 50.0%
! CGTTGCATGTTCTCGAGTTG+TGG - chr5.1:27617575-27617594 None:intergenic 50.0%
! GAAGTATAATGGTGGGGGTG+GGG + chr5.1:27619376-27619395 MS.gene45192:CDS 50.0%
! GCAGGGAAGTATAATGGTGG+GGG + chr5.1:27619371-27619390 MS.gene45192:CDS 50.0%
! GCATGACTTGCAACCTGAGT+TGG - chr5.1:27618021-27618040 None:intergenic 50.0%
! GCTGCAGGGAAGTATAATGG+TGG + chr5.1:27619368-27619387 MS.gene45192:CDS 50.0%
! GGAAGTATAATGGTGGGGGT+GGG + chr5.1:27619375-27619394 MS.gene45192:CDS 50.0%
! TTTTCCGATGGGTCTTGGTC+CGG + chr5.1:27619106-27619125 MS.gene45192:CDS 50.0%
!! AGGGTTGTTATTGGTGGTGG+TGG - chr5.1:27617670-27617689 None:intergenic 50.0%
!! GAAGGAGGAAGCATTGGTGT+TGG - chr5.1:27619179-27619198 None:intergenic 50.0%
!! GACGAATGGCTGCTACTTTC+TGG - chr5.1:27619314-27619333 None:intergenic 50.0%
!! GGGTTGCAACAGGTTGGTAA+AGG + chr5.1:27617952-27617971 MS.gene45192:CDS 50.0%
!! GTCGTCATCGGCTTCATCAT+CGG + chr5.1:27619331-27619350 MS.gene45192:CDS 50.0%
!! GTTGTTATTGGTGGTGGTGG+TGG - chr5.1:27617667-27617686 None:intergenic 50.0%
!! TGAAGGTGTTCCTTGGACAG+AGG + chr5.1:27617906-27617925 MS.gene45192:intron 50.0%
!! TGGTGTTGGCCTAATTGGCT+TGG - chr5.1:27619165-27619184 None:intergenic 50.0%
!! TGGTTGGTGAAGGTGTTCCT+TGG + chr5.1:27617899-27617918 MS.gene45192:intron 50.0%
ATACATAACCGGCATCCGCG+GGG - chr5.1:27617758-27617777 None:intergenic 55.0%
CAAAGAGACTGGATCTCCGG+CGG - chr5.1:27618083-27618102 None:intergenic 55.0%
CACATTATGGCGGCATCCCT+GGG + chr5.1:27619078-27619097 MS.gene45192:CDS 55.0%
CGATCCAGAGAACGCAAACG+AGG + chr5.1:27617804-27617823 MS.gene45192:CDS 55.0%
CTGACCTCGTTTGCGTTCTC+TGG - chr5.1:27617811-27617830 None:intergenic 55.0%
GATACATAACCGGCATCCGC+GGG - chr5.1:27617759-27617778 None:intergenic 55.0%
GATGACATCGTTCACGCCTC+TGG + chr5.1:27617780-27617799 MS.gene45192:CDS 55.0%
GGACCAAGACCCATCGGAAA+AGG - chr5.1:27619107-27619126 None:intergenic 55.0%
GGATACATAACCGGCATCCG+CGG - chr5.1:27617760-27617779 None:intergenic 55.0%
GGGTTGGAATCTTGTGGAGG+TGG - chr5.1:27617738-27617757 None:intergenic 55.0%
GTGAAGATGGATATGCGGCG+GGG - chr5.1:27619061-27619080 None:intergenic 55.0%
GTTACCGGACCAAGACCCAT+CGG - chr5.1:27619113-27619132 None:intergenic 55.0%
TCACATTATGGCGGCATCCC+TGG + chr5.1:27619077-27619096 MS.gene45192:CDS 55.0%
TCCACAAGATTCCAACCCCG+CGG + chr5.1:27617740-27617759 MS.gene45192:CDS 55.0%
TCGAGAACATGCAACGACGG+TGG + chr5.1:27617579-27617598 MS.gene45192:CDS 55.0%
TGAAGATGGATATGCGGCGG+GGG - chr5.1:27619060-27619079 None:intergenic 55.0%
TGCTCACAGTGCGGCAACAA+CGG + chr5.1:27617549-27617568 MS.gene45192:CDS 55.0%
! CCTGGGACACCTTTTCCGAT+GGG + chr5.1:27619095-27619114 MS.gene45192:CDS 55.0%
! GGGAAGTATAATGGTGGGGG+TGG + chr5.1:27619374-27619393 MS.gene45192:CDS 55.0%
! GTTGGGGTGGTTGTTTCCTC+CGG - chr5.1:27617643-27617662 None:intergenic 55.0%
!! ATTGGTGGTGGTGGTGGTGT+TGG - chr5.1:27617661-27617680 None:intergenic 55.0%
!! CCATCGGAAAAGGTGTCCCA+GGG - chr5.1:27619097-27619116 None:intergenic 55.0%
!! TTGGTGGTGGTGGTGGTGTT+GGG - chr5.1:27617660-27617679 None:intergenic 55.0%
!! TTTGGTGTTCGACTCACCGG+AGG + chr5.1:27617624-27617643 MS.gene45192:CDS 55.0%
GCGGGGTTGGAATCTTGTGG+AGG - chr5.1:27617741-27617760 None:intergenic 60.0%
GCGTTCTCTGGATCGACCAG+AGG - chr5.1:27617799-27617818 None:intergenic 60.0%
TCCGCGGGGTTGGAATCTTG+TGG - chr5.1:27617744-27617763 None:intergenic 60.0%
TCTCGCACGTGCTCACAGTG+CGG + chr5.1:27617540-27617559 MS.gene45192:CDS 60.0%
! CCCTGGGACACCTTTTCCGA+TGG + chr5.1:27619094-27619113 MS.gene45192:CDS 60.0%
! GGCGGCGGTTCTGGTTATGA+CGG - chr5.1:27618062-27618081 None:intergenic 60.0%
! GGCGGTTCTGGTTATGACGG+CGG - chr5.1:27618059-27618078 None:intergenic 60.0%
! GGTTCTGGTTATGACGGCGG+AGG - chr5.1:27618056-27618075 None:intergenic 60.0%
!! CCCATCGGAAAAGGTGTCCC+AGG - chr5.1:27619098-27619117 None:intergenic 60.0%
!! TGGTGGTGGTGGTGGTGTTG+GGG - chr5.1:27617659-27617678 None:intergenic 60.0%
AGAGACTGGATCTCCGGCGG+CGG - chr5.1:27618080-27618099 None:intergenic 65.0%
ATAACCAGAACCGCCGCCGC+CGG + chr5.1:27618064-27618083 MS.gene45192:CDS 65.0%
ATAACCGGCATCCGCGGGGT+TGG - chr5.1:27617754-27617773 None:intergenic 65.0%
GATGGATATGCGGCGGGGGT+TGG - chr5.1:27619056-27619075 None:intergenic 65.0%
GATTCCAACCCCGCGGATGC+CGG + chr5.1:27617747-27617766 MS.gene45192:CDS 65.0%
!! TGGTGGTGGTGGTGTTGGGG+TGG - chr5.1:27617656-27617675 None:intergenic 65.0%
GGATATGCGGCGGGGGTTGG+TGG - chr5.1:27619053-27619072 None:intergenic 70.0%
!! TATGCGGCGGGGGTTGGTGG+TGG - chr5.1:27619050-27619069 None:intergenic 70.0%
ATCTCCGGCGGCGGCGGTTC+TGG - chr5.1:27618071-27618090 None:intergenic 75.0%
GACTGGATCTCCGGCGGCGG+CGG - chr5.1:27618077-27618096 None:intergenic 75.0%
!! GCGGGGGTTGGTGGTGGCAG+TGG - chr5.1:27619044-27619063 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 27617531 27619421 27617531 ID=MS.gene45192
chr5.1 mRNA 27617531 27619421 27617531 ID=MS.gene45192.t1;Parent=MS.gene45192
chr5.1 exon 27617531 27617825 27617531 ID=MS.gene45192.t1.exon1;Parent=MS.gene45192.t1
chr5.1 CDS 27617531 27617825 27617531 ID=cds.MS.gene45192.t1;Parent=MS.gene45192.t1
chr5.1 exon 27617911 27618119 27617911 ID=MS.gene45192.t1.exon2;Parent=MS.gene45192.t1
chr5.1 CDS 27617911 27618119 27617911 ID=cds.MS.gene45192.t1;Parent=MS.gene45192.t1
chr5.1 exon 27618984 27619421 27618984 ID=MS.gene45192.t1.exon3;Parent=MS.gene45192.t1
chr5.1 CDS 27618984 27619421 27618984 ID=cds.MS.gene45192.t1;Parent=MS.gene45192.t1
Gene Sequence

>MS.gene45192

ATGTCTTTGTCTCGCACGTGCTCACAGTGCGGCAACAACGGCCACAACTCGAGAACATGCAACGACGGTGGAGAAGAAAAAAGCATCATGATTTTTGGTGTTCGACTCACCGGAGGAAACAACCACCCCAACACCACCACCACCACCAATAACAACCCTTTCAGAAAAAGTGCTAGCATGACCAATCTCTCTCAGTATGAACAACCACCTCCACAAGATTCCAACCCCGCGGATGCCGGTTATGTATCCGATGACATCGTTCACGCCTCTGGTCGATCCAGAGAACGCAAACGAGGTCAGTATTTAAACTAGATACATCAACTTTTGTTTGATATATTTTTGCTTATGTATATTAATGTTTTTTGTTTTGGTTGGTGAAGGTGTTCCTTGGACAGAGGAAGAACACAAGCTCTTCTTGTTGGGGTTGCAACAGGTTGGTAAAGGTGATTGGAGAGGAATTTCTAGAAACTTTGTGAAAACAAGGACACCAACTCAGGTTGCAAGTCATGCTCAGAAGTATTTCCTCCGCCGTCATAACCAGAACCGCCGCCGCCGGAGATCCAGTCTCTTTGATATTACCACCGATACGGTAATATTTGTTCTTTATTTTAATCTTTTTTAATAATTTCCTTTTATATTTTTAACAATTTCCTTTTCTAATAATCTGATTTTATTAGTCTAGTCAAAAATGAAAAAACTAATTTCCTTAAATATATTTCGTATAATTTTACTTTTCAAAGTAATAATGATGATGATGATAAATAATTTAATAATAATGTAATTTTTATACAAATATATAATGCTCATTTATTTTATTATATAAATATTAATGATGATCATGATAAATAATTAATAATAATGTAATTTTTATACATATATATAATCATAATTTATTTTATTATATCTTGTCAAAAAAAATTATTTTATTATATAGATATTAGATTTAATTATATATTATTAATGTAGAATATTTTTATATTATGAACCAATTGTAATCATGATATATTAATTTTTTATCTTTTTATATTAGTTATAATTAAATTTAAAATTGCATATATGTTTGACTGTGATTTAAAATTTCTTGACGACGATTTAATCACAAAATTTATTGACGATTTATCAATTGAATTTATAAACATGTCTAATATATTTTCAACACAAATAATAATGTGACATGAGTAAAAAATAAAAATTACATATGTTTTTTTAACTATTTCGTTTTACTATAAGAGGATTCATTCTTCCTATCAATTAACATAAGCTCATGGTGACTTTTTGTACTTGTATTTTACTCCCTCCGTCCCAAATATATTATCTCCGGACATATATTTTTGCGAACATGACAATTATTATGATAAAGAGATTGTACTGTTTTATTAAAATTATCAAGAGACTGTACTGTCGTTATTAAAATTATCATTAATGTAATTTTGCCTTGGATTATTTATTTTAGGTGATGGAACCTTCAACTATAATGGAAGATGATCAATTTCAGCAAGAAACAGTGGCGCCACTGCCACCACCAACCCCCGCCGCATATCCATCTTCACATTATGGCGGCATCCCTGGGACACCTTTTCCGATGGGTCTTGGTCCGGTAACATTGCCGGTAATGAGTGCTGAAAGAGTGGCCAAGCCAATTAGGCCAACACCAATGCTTCCTCCTTCTTCTAAGATGGCTAATCTGAACTTGAAAGACAAAGCTTCTTCTTCTTCTTCCATTGAGCCTTTTCCATTGTCATTGAAGCTGCAAACTTCGGATCCATCGGAAGATCATTCACCAGAAAGTAGCAGCCATTCGTCGTCATCGGCTTCATCATCGGCTTTTAAAACCATGGCTGCAGGGAAGTATAATGGTGGGGGTGGGGATAGCATTATCAGTGTTGCTTGA
Protein sequence

>MS.gene45192.t1

MSLSRTCSQCGNNGHNSRTCNDGGEEKSIMIFGVRLTGGNNHPNTTTTTNNNPFRKSASMTNLSQYEQPPPQDSNPADAGYVSDDIVHASGRSRERKRGVPWTEEEHKLFLLGLQQVGKGDWRGISRNFVKTRTPTQVASHAQKYFLRRHNQNRRRRRSSLFDITTDTVMEPSTIMEDDQFQQETVAPLPPPTPAAYPSSHYGGIPGTPFPMGLGPVTLPVMSAERVAKPIRPTPMLPPSSKMANLNLKDKASSSSSIEPFPLSLKLQTSDPSEDHSPESSSHSSSSASSSAFKTMAAGKYNGGGGDSIISVA