Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057577.t1 | XP_003621282.2 | 60.6 | 218 | 45 | 3 | 1 | 178 | 1 | 217 | 2.50E-22 | 115.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057577.t1 | G7L237 | 60.6 | 218 | 45 | 3 | 1 | 178 | 1 | 217 | 1.8e-22 | 115.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057576 | MS.gene057577 | 0.906558 | 1.31E-80 | -1.69E-46 |
| MS.gene057577 | MS.gene057584 | 0.910542 | 1.67E-82 | -1.69E-46 |
| MS.gene057577 | MS.gene057586 | 0.870048 | 1.96E-66 | -1.69E-46 |
| MS.gene057577 | MS.gene057588 | 0.948918 | 3.63E-107 | -1.69E-46 |
| MS.gene057577 | MS.gene057591 | 0.903345 | 3.82E-79 | -1.69E-46 |
| MS.gene057577 | MS.gene063783 | 0.803724 | 3.04E-49 | -1.69E-46 |
| MS.gene057577 | MS.gene45357 | 0.905241 | 5.28E-80 | -1.69E-46 |
| MS.gene057577 | MS.gene49515 | 0.882398 | 1.08E-70 | -1.69E-46 |
| MS.gene057577 | MS.gene49520 | 0.886286 | 3.90E-72 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057577.t1 | MTR_7g011390 | 69.266 | 218 | 26 | 4 | 1 | 178 | 1 | 217 | 6.35e-73 | 219 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGGCTGCTGCTGGCATCCA+AGG | 0.270786 | 7.2:+85867972 | None:intergenic |
| ATCCAACGGCTGGCTGCTGC+TGG | 0.310560 | 7.2:+85867963 | None:intergenic |
| TCTAAATTCACTCTGGTCAT+TGG | 0.364019 | 7.2:-85868767 | MS.gene057577:CDS |
| TGAGGATAAAGCTGCTGTTC+AGG | 0.399727 | 7.2:-85867908 | MS.gene057577:CDS |
| TGGCTGCTGCTGGCATCCAA+GGG | 0.406161 | 7.2:+85867973 | None:intergenic |
| GGTCCAATATCTAGAGTAGT+TGG | 0.437672 | 7.2:+85867866 | None:intergenic |
| TTATGATCAGATTTATCGTC+AGG | 0.438391 | 7.2:-85869129 | MS.gene057577:intron |
| CGAAAATGATATGAAGCCCT+TGG | 0.474824 | 7.2:-85867989 | MS.gene057577:CDS |
| ATAAAGCTGCTGTTCAGGAG+GGG | 0.477624 | 7.2:-85867903 | MS.gene057577:CDS |
| TGCCAGCAGCAGCCAGCCGT+TGG | 0.479830 | 7.2:-85867965 | MS.gene057577:CDS |
| ACTCAGTTTCTTCTTAGACT+TGG | 0.487236 | 7.2:+85869321 | None:intergenic |
| GCAGGCCCGCAAAGCAAAAG+AGG | 0.498850 | 7.2:-85868809 | MS.gene057577:CDS |
| TCGTTTGTTCCCATGGGTGG+AGG | 0.504985 | 7.2:-85869370 | None:intergenic |
| AACTCGTTTGTTCCCATGGG+TGG | 0.508005 | 7.2:-85869373 | None:intergenic |
| GTCCAATATCTAGAGTAGTT+GGG | 0.512418 | 7.2:+85867867 | None:intergenic |
| TAAAGCTGCTGTTCAGGAGG+GGG | 0.518520 | 7.2:-85867902 | MS.gene057577:CDS |
| AGCCTCTTCTGCTAATCGAC+GGG | 0.530366 | 7.2:+85868849 | None:intergenic |
| GATAAAGCTGCTGTTCAGGA+GGG | 0.535418 | 7.2:-85867904 | MS.gene057577:CDS |
| TGTTGAGCTCATCCAACGGC+TGG | 0.538954 | 7.2:+85867953 | None:intergenic |
| AGCCCGTCGATTAGCAGAAG+AGG | 0.554670 | 7.2:-85868851 | MS.gene057577:CDS |
| CTCTTGCTCTAAATTCACTC+TGG | 0.587930 | 7.2:-85868774 | MS.gene057577:CDS |
| GAGCCTCTTCTGCTAATCGA+CGG | 0.589148 | 7.2:+85868848 | None:intergenic |
| GACTTGTTGAGCTCATCCAA+CGG | 0.593483 | 7.2:+85867949 | None:intergenic |
| AAAGCTGCTGTTCAGGAGGG+GGG | 0.608878 | 7.2:-85867901 | MS.gene057577:CDS |
| GGATAAAGCTGCTGTTCAGG+AGG | 0.611925 | 7.2:-85867905 | MS.gene057577:CDS |
| GAACAGCAGCTTTATCCTCA+AGG | 0.621167 | 7.2:+85867911 | None:intergenic |
| AACACAAAACGTTAGCCTTG+AGG | 0.622562 | 7.2:-85867926 | MS.gene057577:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTAAATGTTATGAAAAT+TGG | + | chr7.2:85868022-85868041 | None:intergenic | 15.0% |
| !! | TATGATATGAATTGTTAATT+TGG | - | chr7.2:85868212-85868231 | MS.gene057577:intron | 15.0% |
| !!! | ATTTTTAATTATGTATTTGC+AGG | - | chr7.2:85869215-85869234 | MS.gene057577:intron | 15.0% |
| !! | TTGTAAGAAATTAAGCAAAA+GGG | - | chr7.2:85869182-85869201 | MS.gene057577:intron | 20.0% |
| !!! | AAAAATAAAAGTCTTGTAGA+AGG | + | chr7.2:85868889-85868908 | None:intergenic | 20.0% |
| !!! | ATGAATTGTTAATTTGGTTA+TGG | - | chr7.2:85868218-85868237 | MS.gene057577:intron | 20.0% |
| !!! | CATAGCTTTTCTTTAAAATT+GGG | - | chr7.2:85869082-85869101 | MS.gene057577:intron | 20.0% |
| !!! | TTTTAACTAACTTGTTATGT+TGG | - | chr7.2:85868137-85868156 | MS.gene057577:intron | 20.0% |
| ! | AATTTCTTACAAGTAAGGAA+AGG | + | chr7.2:85869174-85869193 | None:intergenic | 25.0% |
| ! | CTTGTAAGAAATTAAGCAAA+AGG | - | chr7.2:85869181-85869200 | MS.gene057577:intron | 25.0% |
| ! | TGCTTAATTTCTTACAAGTA+AGG | + | chr7.2:85869179-85869198 | None:intergenic | 25.0% |
| !! | GTTTTCTTAAAAATTCCTCT+AGG | - | chr7.2:85868741-85868760 | MS.gene057577:CDS | 25.0% |
| !! | GTTTTCTTAAAAATTGCTCT+AGG | - | chr7.2:85868577-85868596 | MS.gene057577:intron | 25.0% |
| !! | TTTTGCAAGTTTTCGAAAAA+GGG | + | chr7.2:85868702-85868721 | None:intergenic | 25.0% |
| !!! | ACTTGGATTTCTTTTTTGTT+CGG | + | chr7.2:85867899-85867918 | None:intergenic | 25.0% |
| !!! | CCAATTTTAAAGAAAAGCTA+TGG | + | chr7.2:85869084-85869103 | None:intergenic | 25.0% |
| !!! | CCATAGCTTTTCTTTAAAAT+TGG | - | chr7.2:85869081-85869100 | MS.gene057577:intron | 25.0% |
| !!! | GCAATTTTTAAGAAAACCTA+CGG | + | chr7.2:85868574-85868593 | None:intergenic | 25.0% |
| !!! | GGAATTTTTAAGAAAACCTA+CGG | + | chr7.2:85868738-85868757 | None:intergenic | 25.0% |
| AGATGAAAAGTGAATAGAAG+TGG | + | chr7.2:85869016-85869035 | None:intergenic | 30.0% | |
| ATAGATAGAAAGATAGAGAG+AGG | + | chr7.2:85867944-85867963 | None:intergenic | 30.0% | |
| CTTCTTACTAATACACCAAA+TGG | - | chr7.2:85869108-85869127 | MS.gene057577:intron | 30.0% | |
| GAATAGAAGTGGTATACTTA+TGG | + | chr7.2:85869005-85869024 | None:intergenic | 30.0% | |
| GATAGTACCTACAAATACAA+TGG | + | chr7.2:85868327-85868346 | None:intergenic | 30.0% | |
| TCATCTTCCATTGTATTTGT+AGG | - | chr7.2:85868317-85868336 | MS.gene057577:intron | 30.0% | |
| TTATGATCAGATTTATCGTC+AGG | - | chr7.2:85868105-85868124 | MS.gene057577:intron | 30.0% | |
| ! | CTTTTGCAAGTTTTCGAAAA+AGG | + | chr7.2:85868703-85868722 | None:intergenic | 30.0% |
| ACTCAGTTTCTTCTTAGACT+TGG | + | chr7.2:85867916-85867935 | None:intergenic | 35.0% | |
| CAAAAAAGTAGCAGAAGAAG+AGG | - | chr7.2:85868359-85868378 | MS.gene057577:intron | 35.0% | |
| TCAACTAATATCCAGACACT+AGG | + | chr7.2:85868957-85868976 | None:intergenic | 35.0% | |
| TCTAAATTCACTCTGGTCAT+TGG | - | chr7.2:85868467-85868486 | MS.gene057577:intron | 35.0% | |
| TCTTGCTCAAAAAAAACTGC+AGG | - | chr7.2:85868407-85868426 | MS.gene057577:intron | 35.0% | |
| ! | AGCGTTTTTGTAGTCATGTT+TGG | + | chr7.2:85868519-85868538 | None:intergenic | 35.0% |
| ! | ATGGATTGACATGAATGTTC+AGG | + | chr7.2:85868986-85869005 | None:intergenic | 35.0% |
| ! | CATGATGATGATGATGATCT+TGG | - | chr7.2:85868257-85868276 | MS.gene057577:intron | 35.0% |
| ! | GCGTTTTTGTAGTCATGTTT+GGG | + | chr7.2:85868518-85868537 | None:intergenic | 35.0% |
| ! | TTTTGTAATCTGTATGCAGC+TGG | - | chr7.2:85868661-85868680 | MS.gene057577:intron | 35.0% |
| ! | TTTTGTAGTCATGTTTGGGT+AGG | + | chr7.2:85868514-85868533 | None:intergenic | 35.0% |
| !! | AAGCACGTTGTTTCTTAGTA+AGG | + | chr7.2:85868618-85868637 | None:intergenic | 35.0% |
| !! | CGTTGTTTCTTAGTAAGGAA+AGG | + | chr7.2:85868613-85868632 | None:intergenic | 35.0% |
| !! | GAAAAAGGGTATCATCTTCA+TGG | + | chr7.2:85868688-85868707 | None:intergenic | 35.0% |
| !!! | CTCTTCTTCTGCTACTTTTT+TGG | + | chr7.2:85868361-85868380 | None:intergenic | 35.0% |
| AAAGGACAAGACAATCCTAG+AGG | + | chr7.2:85868595-85868614 | None:intergenic | 40.0% | |
| AAATCTACAAACACAGCCGT+AGG | - | chr7.2:85868555-85868574 | MS.gene057577:CDS | 40.0% | |
| AACAGGAGATAGGTTTATGC+AGG | + | chr7.2:85868924-85868943 | None:intergenic | 40.0% | |
| CAGTTTCATGAACAGGAGAT+AGG | + | chr7.2:85868934-85868953 | None:intergenic | 40.0% | |
| CATGTTCTGTTCTGTTCTGA+TGG | - | chr7.2:85868162-85868181 | MS.gene057577:intron | 40.0% | |
| CGAAAATGATATGAAGCCCT+TGG | - | chr7.2:85869245-85869264 | MS.gene057577:intron | 40.0% | |
| GATAAGAAGACAGTCCTAGA+GGG | + | chr7.2:85869152-85869171 | None:intergenic | 40.0% | |
| ! | CTCTTGCTCTAAATTCACTC+TGG | - | chr7.2:85868460-85868479 | MS.gene057577:intron | 40.0% |
| !! | AACACAAAACGTTAGCCTTG+AGG | - | chr7.2:85869308-85869327 | MS.gene057577:intron | 40.0% |
| !! | TGATGATCTTGGCTCTGTAT+AGG | - | chr7.2:85868268-85868287 | MS.gene057577:intron | 40.0% |
| AAAGCTACAAACACAGCCGT+AGG | - | chr7.2:85868719-85868738 | MS.gene057577:CDS | 45.0% | |
| ACCTCCTTGCCATATTGTGT+TGG | + | chr7.2:85868863-85868882 | None:intergenic | 45.0% | |
| ATAAAGCTGCTGTTCAGGAG+GGG | - | chr7.2:85869331-85869350 | MS.gene057577:CDS | 45.0% | |
| CACTAGGCAGTTTCATGAAC+AGG | + | chr7.2:85868941-85868960 | None:intergenic | 45.0% | |
| CATGCCAACACAATATGGCA+AGG | - | chr7.2:85868856-85868875 | MS.gene057577:CDS | 45.0% | |
| GATAAAGCTGCTGTTCAGGA+GGG | - | chr7.2:85869330-85869349 | MS.gene057577:CDS | 45.0% | |
| GGATAAGAAGACAGTCCTAG+AGG | + | chr7.2:85869153-85869172 | None:intergenic | 45.0% | |
| GTGCACATGCCAACACAATA+TGG | - | chr7.2:85868851-85868870 | MS.gene057577:CDS | 45.0% | |
| TCATGAAACTGCCTAGTGTC+TGG | - | chr7.2:85868943-85868962 | MS.gene057577:intron | 45.0% | |
| TGAGGATAAAGCTGCTGTTC+AGG | - | chr7.2:85869326-85869345 | MS.gene057577:CDS | 45.0% | |
| ! | GAACAGCAGCTTTATCCTCA+AGG | + | chr7.2:85869326-85869345 | None:intergenic | 45.0% |
| ! | TATTGTGTTGGCATGTGCAC+AGG | + | chr7.2:85868851-85868870 | None:intergenic | 45.0% |
| !! | GACTTGTTGAGCTCATCCAA+CGG | + | chr7.2:85869288-85869307 | None:intergenic | 45.0% |
| !! | TACAAAATAAAAATAAAAAA+TGG | + | chr7.2:85868062-85868081 | None:intergenic | 5.0% |
| AGCCTCTTCTGCTAATCGAC+GGG | + | chr7.2:85868388-85868407 | None:intergenic | 50.0% | |
| CACCAAATGGAGAGAGCCTT+AGG | - | chr7.2:85869121-85869140 | MS.gene057577:intron | 50.0% | |
| GAGCCTCTTCTGCTAATCGA+CGG | + | chr7.2:85868389-85868408 | None:intergenic | 50.0% | |
| GCCAACACAATATGGCAAGG+AGG | - | chr7.2:85868859-85868878 | MS.gene057577:CDS | 50.0% | |
| GGATAAAGCTGCTGTTCAGG+AGG | - | chr7.2:85869329-85869348 | MS.gene057577:CDS | 50.0% | |
| TAAAGCTGCTGTTCAGGAGG+GGG | - | chr7.2:85869332-85869351 | MS.gene057577:CDS | 50.0% | |
| TGCCTAAGGCTCTCTCCATT+TGG | + | chr7.2:85869126-85869145 | None:intergenic | 50.0% | |
| ! | CTGCTGCCTCTTTTGCTTTG+CGG | + | chr7.2:85868434-85868453 | None:intergenic | 50.0% |
| ! | GGCATGTGCACAGGTTGAAT+TGG | + | chr7.2:85868842-85868861 | None:intergenic | 50.0% |
| ! | TGCTGCCTCTTTTGCTTTGC+GGG | + | chr7.2:85868433-85868452 | None:intergenic | 50.0% |
| AAAGCTGCTGTTCAGGAGGG+GGG | - | chr7.2:85869333-85869352 | MS.gene057577:CDS | 55.0% | |
| AGCCTTAGGCAATGCCCTCT+AGG | - | chr7.2:85869135-85869154 | MS.gene057577:CDS | 55.0% | |
| TGTTGAGCTCATCCAACGGC+TGG | + | chr7.2:85869284-85869303 | None:intergenic | 55.0% | |
| ! | AGCCCGTCGATTAGCAGAAG+AGG | - | chr7.2:85868383-85868402 | MS.gene057577:intron | 55.0% |
| !! | GTCCTAGAGGGCATTGCCTA+AGG | + | chr7.2:85869140-85869159 | None:intergenic | 55.0% |
| GCAGGCCCGCAAAGCAAAAG+AGG | - | chr7.2:85868425-85868444 | MS.gene057577:intron | 60.0% | |
| TGGCTGCTGCTGGCATCCAA+GGG | + | chr7.2:85869264-85869283 | None:intergenic | 60.0% | |
| ! | GCTGTTCAGGAGGGGGGTAT+TGG | - | chr7.2:85869339-85869358 | MS.gene057577:CDS | 60.0% |
| CTGGCTGCTGCTGGCATCCA+AGG | + | chr7.2:85869265-85869284 | None:intergenic | 65.0% | |
| ! | ATCCAACGGCTGGCTGCTGC+TGG | + | chr7.2:85869274-85869293 | None:intergenic | 65.0% |
| TGCCAGCAGCAGCCAGCCGT+TGG | - | chr7.2:85869269-85869288 | MS.gene057577:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 85867876 | 85869380 | 85867876 | ID=MS.gene057577 |
| chr7.2 | mRNA | 85867876 | 85869380 | 85867876 | ID=MS.gene057577.t1;Parent=MS.gene057577 |
| chr7.2 | exon | 85869323 | 85869380 | 85869323 | ID=MS.gene057577.t1.exon1;Parent=MS.gene057577.t1 |
| chr7.2 | CDS | 85869323 | 85869380 | 85869323 | ID=cds.MS.gene057577.t1;Parent=MS.gene057577.t1 |
| chr7.2 | exon | 85869130 | 85869173 | 85869130 | ID=MS.gene057577.t1.exon2;Parent=MS.gene057577.t1 |
| chr7.2 | CDS | 85869130 | 85869173 | 85869130 | ID=cds.MS.gene057577.t1;Parent=MS.gene057577.t1 |
| chr7.2 | exon | 85868684 | 85868917 | 85868684 | ID=MS.gene057577.t1.exon3;Parent=MS.gene057577.t1 |
| chr7.2 | CDS | 85868684 | 85868917 | 85868684 | ID=cds.MS.gene057577.t1;Parent=MS.gene057577.t1 |
| chr7.2 | exon | 85868520 | 85868576 | 85868520 | ID=MS.gene057577.t1.exon4;Parent=MS.gene057577.t1 |
| chr7.2 | CDS | 85868520 | 85868576 | 85868520 | ID=cds.MS.gene057577.t1;Parent=MS.gene057577.t1 |
| chr7.2 | exon | 85867876 | 85868019 | 85867876 | ID=MS.gene057577.t1.exon5;Parent=MS.gene057577.t1 |
| chr7.2 | CDS | 85867876 | 85868019 | 85867876 | ID=cds.MS.gene057577.t1;Parent=MS.gene057577.t1 |
| Gene Sequence |
| Protein sequence |
