AlfalfaGEDB | MS.gene45557

M. sativa cultivar XinJiangDaYe / MS.gene45557

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF01428.19 PF01754.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45557.t1	XP_024632562.1	97.7	172	4	0	1	172	1	172	1.60E-90	342

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45557.t1	A2YEZ6	70.1	174	47	3	1	172	1	171	2.1e-64	246.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45557.t1	B7FGP6	97.7	172	4	0	1	172	1	172	1.1e-90	342.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050623	MS.gene45557	0.845175	4.79E-59	-1.69E-46
MS.gene051662	MS.gene45557	-0.822794	1.96E-53	-1.69E-46
MS.gene052048	MS.gene45557	0.99031	4.97E-182	-1.69E-46
MS.gene054942	MS.gene45557	-0.815081	1.11E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene050335	MS.gene45557	PPI
MS.gene45557	MS.gene24724	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45557.t1	MTR_2g098160	97.674	172	4	0	1	172	1	172	2.81e-124	347
MS.gene45557.t1	MTR_0249s0070	61.765	170	61	2	3	172	4	169	2.54e-76	225
MS.gene45557.t1	MTR_0249s0070	61.765	170	61	2	3	172	12	177	2.72e-76	226
MS.gene45557.t1	MTR_4g065570	77.622	143	25	2	31	172	1	137	1.36e-75	222
MS.gene45557.t1	MTR_7g092400	63.743	171	59	3	3	172	92	260	1.55e-75	227
MS.gene45557.t1	MTR_7g104320	46.061	165	79	3	12	172	10	168	1.59e-45	147
MS.gene45557.t1	MTR_4g053440	44.643	168	86	4	8	172	7	170	1.89e-42	139
MS.gene45557.t1	MTR_2g086190	44.304	158	71	5	15	172	10	150	4.60e-41	135
MS.gene45557.t1	MTR_1g060380	44.785	163	74	4	11	172	9	156	1.92e-37	126
MS.gene45557.t1	MTR_3g028010	37.821	156	77	2	17	172	28	163	7.51e-29	104
MS.gene45557.t1	MTR_8g036980	52.222	90	29	1	83	172	76	151	1.06e-27	101
MS.gene45557.t1	MTR_1g100773	33.333	156	84	4	15	170	3	138	7.22e-26	96.7
MS.gene45557.t1	MTR_7g114920	38.065	155	69	6	15	169	3	130	6.42e-25	94.0
MS.gene45557.t1	MTR_2g054650	33.333	156	70	5	15	170	4	125	6.32e-20	80.9
MS.gene45557.t1	MTR_3g025570	70.455	44	13	0	107	150	4	47	3.67e-19	78.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45557.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene45557.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene45557.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene45557.t1	AT1G51200	60.452	177	61	4	1	172	1	173	1.16e-62	191
MS.gene45557.t1	AT2G36320	47.879	165	71	4	11	172	9	161	4.94e-46	148
MS.gene45557.t1	AT4G12040	42.683	164	90	2	12	172	13	175	1.71e-45	147
MS.gene45557.t1	AT4G12040	42.683	164	90	2	12	172	13	175	1.71e-45	147
MS.gene45557.t1	AT2G27580	40.123	162	85	2	15	172	10	163	3.76e-42	139
MS.gene45557.t1	AT2G27580	40.123	162	85	2	15	172	10	163	3.76e-42	139
MS.gene45557.t1	AT1G12440	40.123	162	92	2	11	172	12	168	6.01e-40	133
MS.gene45557.t1	AT1G12440	40.123	162	92	2	11	172	12	168	6.01e-40	133
MS.gene45557.t1	AT1G12440	40.123	162	92	2	11	172	12	168	6.01e-40	133
MS.gene45557.t1	AT3G52800	43.210	162	92	0	11	172	9	170	7.58e-38	128
MS.gene45557.t1	AT3G52800	43.210	162	92	0	11	172	9	170	7.58e-38	128
MS.gene45557.t1	AT4G22820	38.509	161	93	3	15	172	19	176	6.48e-37	125
MS.gene45557.t1	AT4G22820	38.509	161	93	3	15	172	19	176	6.48e-37	125
MS.gene45557.t1	AT3G12630	39.333	150	71	2	23	172	31	160	7.01e-32	112
MS.gene45557.t1	AT4G14225	37.342	158	62	5	15	172	5	125	2.84e-27	99.8
MS.gene45557.t1	AT4G25380	32.903	155	70	4	18	172	10	130	1.58e-24	92.8

Find 48 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTTCTGCAGAGATGGTTT+TGG	0.250126	2.1:+6133112	None:intergenic
GTTTCAGCATCATGTCTTTG+TGG	0.367335	2.1:+6133255	None:intergenic
CATCATGTCTTTGTGGCACT+TGG	0.399820	2.1:+6133262	None:intergenic
TTCCGGCTCAGAGGAGAGTT+TGG	0.400683	2.1:-6133081	MS.gene45557:CDS
CCAGCAGTGCGGTAATCAAA+TGG	0.411400	2.1:+6132930	None:intergenic
GCAGAACCGTTAGTTGCTTC+CGG	0.413723	2.1:-6133098	MS.gene45557:CDS
AATAAGCGGGTTGGTTTGAC+AGG	0.421004	2.1:-6133017	MS.gene45557:CDS
CAAAGACATGATGCTGAAAC+AGG	0.424095	2.1:-6133252	MS.gene45557:CDS
CACTTGCAGCATCTTCCATT+GGG	0.430249	2.1:-6133220	MS.gene45557:CDS
TTGCAGTTGCTGCACCGCTT+AGG	0.445309	2.1:+6133038	None:intergenic
TCCATTGGGAACATCATGAA+TGG	0.454077	2.1:-6133206	MS.gene45557:CDS
CCATTTGATTACCGCACTGC+TGG	0.477078	2.1:-6132930	MS.gene45557:CDS
ATGCACAACATAGGACCCTC+TGG	0.486214	2.1:+6133332	None:intergenic
ACACTTGCAGCATCTTCCAT+TGG	0.488087	2.1:-6133221	MS.gene45557:CDS
CTTCACATCCAGTCTGATCA+TGG	0.503432	2.1:+6133360	None:intergenic
CAGCAGTGCGGTAATCAAAT+GGG	0.506125	2.1:+6132931	None:intergenic
CACCGCTTAGGACCATCCTT+CGG	0.507315	2.1:+6133050	None:intergenic
TCCACGCTGGCAGTGATAGC+AGG	0.514469	2.1:+6133158	None:intergenic
CTCCAAACTCTCCTCTGAGC+CGG	0.515481	2.1:+6133079	None:intergenic
AACTAACGGTTCTGCAGAGA+TGG	0.516940	2.1:+6133106	None:intergenic
AGGTTCAATTACACTGCTGC+TGG	0.521288	2.1:+6133178	None:intergenic
GTGCAGCAACTGCAATAAGC+GGG	0.530850	2.1:-6133030	MS.gene45557:CDS
CCATTGGGAACATCATGAAT+GGG	0.543973	2.1:-6133205	MS.gene45557:CDS
AAAAGCAAACCCTGTTGTGA+AGG	0.563268	2.1:-6132892	MS.gene45557:CDS
GCACTTGGAACACATGTTCA+TGG	0.566316	2.1:+6133277	None:intergenic
TTGGAGAAGAAGCCGAAGGA+TGG	0.571839	2.1:-6133062	MS.gene45557:CDS
CCCATTCATGATGTTCCCAA+TGG	0.580215	2.1:+6133205	None:intergenic
TGCACAACATAGGACCCTCT+GGG	0.580560	2.1:+6133333	None:intergenic
AGCGATGTACAGCACAGTAA+AGG	0.582881	2.1:+6132973	None:intergenic
AGCAACTGCAATAAGCGGGT+TGG	0.585228	2.1:-6133026	MS.gene45557:CDS
ACAGGATTTAATTGTCGATG+TGG	0.589267	2.1:-6132999	MS.gene45557:CDS
CATGATGCTGAAACAGGAGC+AGG	0.593551	2.1:-6133246	MS.gene45557:CDS
CAGTTGTTGATGCACAACAT+AGG	0.601548	2.1:+6133323	None:intergenic
GGAGATCTCAGTCGATCAAG+TGG	0.604936	2.1:-6133138	MS.gene45557:CDS
AAGATGGACCATGATCAGAC+TGG	0.612119	2.1:-6133368	MS.gene45557:CDS
GGATGTGAAGCTGCCCCAGA+GGG	0.615247	2.1:-6133347	MS.gene45557:CDS
TCTGAGCCGGAAGCAACTAA+CGG	0.631746	2.1:+6133092	None:intergenic
GAGTTTGGAGAAGAAGCCGA+AGG	0.631849	2.1:-6133066	MS.gene45557:CDS
GGTGCAGCAACTGCAATAAG+CGG	0.632084	2.1:-6133031	MS.gene45557:CDS
AATGGAAGATGCTGCAAGTG+TGG	0.642024	2.1:+6133223	None:intergenic
GTTAGTTGCTTCCGGCTCAG+AGG	0.650978	2.1:-6133090	MS.gene45557:CDS
ATCGACTGAGATCTCCACGC+TGG	0.665451	2.1:+6133145	None:intergenic
TGGATGTGAAGCTGCCCCAG+AGG	0.681846	2.1:-6133348	MS.gene45557:CDS
ACCTGCTATCACTGCCAGCG+TGG	0.686751	2.1:-6133159	MS.gene45557:CDS
AGCCGAAGGATGGTCCTAAG+CGG	0.700565	2.1:-6133052	MS.gene45557:CDS
ATGTTGTGCATCAACAACTG+TGG	0.726175	2.1:-6133320	MS.gene45557:CDS
GCACAACATAGGACCCTCTG+GGG	0.736272	2.1:+6133334	None:intergenic
TTGCATCACGTCCAGCAGTG+CGG	0.742537	2.1:+6132919	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATCAACAACTGTGGATTTTT+CGG	-	chr2.1:6132923-6132942	MS.gene45557:CDS	30.0%
	ACAGGATTTAATTGTCGATG+TGG	-	chr2.1:6133235-6133254	MS.gene45557:CDS	35.0%
	AAAAGCAAACCCTGTTGTGA+AGG	-	chr2.1:6133342-6133361	MS.gene45557:CDS	40.0%
	ATGTTGTGCATCAACAACTG+TGG	-	chr2.1:6132914-6132933	MS.gene45557:CDS	40.0%
	CAAAGACATGATGCTGAAAC+AGG	-	chr2.1:6132982-6133001	MS.gene45557:CDS	40.0%
	CAGTTGTTGATGCACAACAT+AGG	+	chr2.1:6132914-6132933	None:intergenic	40.0%
	CCATTGGGAACATCATGAAT+GGG	-	chr2.1:6133029-6133048	MS.gene45557:CDS	40.0%
	GTTTCAGCATCATGTCTTTG+TGG	+	chr2.1:6132982-6133001	None:intergenic	40.0%
	TCCATTGGGAACATCATGAA+TGG	-	chr2.1:6133028-6133047	MS.gene45557:CDS	40.0%
	AACTAACGGTTCTGCAGAGA+TGG	+	chr2.1:6133131-6133150	None:intergenic	45.0%
	AATGGAAGATGCTGCAAGTG+TGG	+	chr2.1:6133014-6133033	None:intergenic	45.0%
	ACACTTGCAGCATCTTCCAT+TGG	-	chr2.1:6133013-6133032	MS.gene45557:CDS	45.0%
	AGCGATGTACAGCACAGTAA+AGG	+	chr2.1:6133264-6133283	None:intergenic	45.0%
	AGGTTCAATTACACTGCTGC+TGG	+	chr2.1:6133059-6133078	None:intergenic	45.0%
	CACTTGCAGCATCTTCCATT+GGG	-	chr2.1:6133014-6133033	MS.gene45557:CDS	45.0%
	CAGCAGTGCGGTAATCAAAT+GGG	+	chr2.1:6133306-6133325	None:intergenic	45.0%
	CATCATGTCTTTGTGGCACT+TGG	+	chr2.1:6132975-6132994	None:intergenic	45.0%
	CCCATTCATGATGTTCCCAA+TGG	+	chr2.1:6133032-6133051	None:intergenic	45.0%
	CTTCACATCCAGTCTGATCA+TGG	+	chr2.1:6132877-6132896	None:intergenic	45.0%
	GCACTTGGAACACATGTTCA+TGG	+	chr2.1:6132960-6132979	None:intergenic	45.0%
!	AGCTTTTCAGCCTTCACAAC+AGG	+	chr2.1:6133355-6133374	None:intergenic	45.0%
!	GCTTTTCAGCCTTCACAACA+GGG	+	chr2.1:6133354-6133373	None:intergenic	45.0%
!	GGTTCTGCAGAGATGGTTTT+GGG	+	chr2.1:6133124-6133143	None:intergenic	45.0%
!!	AATAAGCGGGTTGGTTTGAC+AGG	-	chr2.1:6133217-6133236	MS.gene45557:CDS	45.0%
	AGCAACTGCAATAAGCGGGT+TGG	-	chr2.1:6133208-6133227	MS.gene45557:CDS	50.0%
	ATGCACAACATAGGACCCTC+TGG	+	chr2.1:6132905-6132924	None:intergenic	50.0%
	CATGATGCTGAAACAGGAGC+AGG	-	chr2.1:6132988-6133007	MS.gene45557:CDS	50.0%
	CCAGCAGTGCGGTAATCAAA+TGG	+	chr2.1:6133307-6133326	None:intergenic	50.0%
	CCATTTGATTACCGCACTGC+TGG	-	chr2.1:6133304-6133323	MS.gene45557:CDS	50.0%
	GAGTTTGGAGAAGAAGCCGA+AGG	-	chr2.1:6133168-6133187	MS.gene45557:CDS	50.0%
	GCAGAACCGTTAGTTGCTTC+CGG	-	chr2.1:6133136-6133155	MS.gene45557:CDS	50.0%
	GGAGATCTCAGTCGATCAAG+TGG	-	chr2.1:6133096-6133115	MS.gene45557:CDS	50.0%
	GGTGCAGCAACTGCAATAAG+CGG	-	chr2.1:6133203-6133222	MS.gene45557:CDS	50.0%
	GTGCAGCAACTGCAATAAGC+GGG	-	chr2.1:6133204-6133223	MS.gene45557:CDS	50.0%
	TCTGAGCCGGAAGCAACTAA+CGG	+	chr2.1:6133145-6133164	None:intergenic	50.0%
	TGCACAACATAGGACCCTCT+GGG	+	chr2.1:6132904-6132923	None:intergenic	50.0%
	TTGGAGAAGAAGCCGAAGGA+TGG	-	chr2.1:6133172-6133191	MS.gene45557:CDS	50.0%
!	CGGTTCTGCAGAGATGGTTT+TGG	+	chr2.1:6133125-6133144	None:intergenic	50.0%
	AGCCGAAGGATGGTCCTAAG+CGG	-	chr2.1:6133182-6133201	MS.gene45557:CDS	55.0%
	ATCGACTGAGATCTCCACGC+TGG	+	chr2.1:6133092-6133111	None:intergenic	55.0%
	CACCGCTTAGGACCATCCTT+CGG	+	chr2.1:6133187-6133206	None:intergenic	55.0%
	CTCCAAACTCTCCTCTGAGC+CGG	+	chr2.1:6133158-6133177	None:intergenic	55.0%
	GCACAACATAGGACCCTCTG+GGG	+	chr2.1:6132903-6132922	None:intergenic	55.0%
	GTTAGTTGCTTCCGGCTCAG+AGG	-	chr2.1:6133144-6133163	MS.gene45557:CDS	55.0%
	TTCCGGCTCAGAGGAGAGTT+TGG	-	chr2.1:6133153-6133172	MS.gene45557:CDS	55.0%
	TTGCAGTTGCTGCACCGCTT+AGG	+	chr2.1:6133199-6133218	None:intergenic	55.0%
	TTGCATCACGTCCAGCAGTG+CGG	+	chr2.1:6133318-6133337	None:intergenic	55.0%
	ACCTGCTATCACTGCCAGCG+TGG	-	chr2.1:6133075-6133094	MS.gene45557:CDS	60.0%
	GGATGTGAAGCTGCCCCAGA+GGG	-	chr2.1:6132887-6132906	MS.gene45557:CDS	60.0%
	TCCACGCTGGCAGTGATAGC+AGG	+	chr2.1:6133079-6133098	None:intergenic	60.0%
	TGGATGTGAAGCTGCCCCAG+AGG	-	chr2.1:6132886-6132905	MS.gene45557:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	6132869	6133387	6132869	ID=MS.gene45557
chr2.1	mRNA	6132869	6133387	6132869	ID=MS.gene45557.t1;Parent=MS.gene45557
chr2.1	exon	6132869	6133387	6132869	ID=MS.gene45557.t1.exon1;Parent=MS.gene45557.t1
chr2.1	CDS	6132869	6133387	6132869	ID=cds.MS.gene45557.t1;Parent=MS.gene45557.t1

Gene Sequence

>MS.gene45557

ATGGACCATGATCAGACTGGATGTGAAGCTGCCCCAGAGGGTCCTATGTTGTGCATCAACAACTGTGGATTTTTCGGAAGCGCAGCTACCATGAACATGTGTTCCAAGTGCCACAAAGACATGATGCTGAAACAGGAGCAGGCCACACTTGCAGCATCTTCCATTGGGAACATCATGAATGGGTCATCCAGCAGCAGTGTAATTGAACCTGCTATCACTGCCAGCGTGGAGATCTCAGTCGATCAAGTGGAGCCCAAAACCATCTCTGCAGAACCGTTAGTTGCTTCCGGCTCAGAGGAGAGTTTGGAGAAGAAGCCGAAGGATGGTCCTAAGCGGTGCAGCAACTGCAATAAGCGGGTTGGTTTGACAGGATTTAATTGTCGATGTGGTAACCTTTACTGTGCTGTACATCGCTACTCAGACAAGCATGACTGCCCATTTGATTACCGCACTGCTGGACGTGATGCAATAGCAAAAGCAAACCCTGTTGTGAAGGCTGAAAAGCTTGACAAGATCTAG

Protein sequence

>MS.gene45557.t1

MDHDQTGCEAAPEGPMLCINNCGFFGSAATMNMCSKCHKDMMLKQEQATLAASSIGNIMNGSSSSSVIEPAITASVEISVDQVEPKTISAEPLVASGSEESLEKKPKDGPKRCSNCNKRVGLTGFNCRCGNLYCAVHRYSDKHDCPFDYRTAGRDAIAKANPVVKAEKLDKI