AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45666

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45666.t1 MTR_4g070190 98.540 137 2 0 1 137 1 137 2.26e-85 246
MS.gene45666.t1 MTR_4g070080 90.361 83 8 0 4 86 2 84 1.02e-52 162
MS.gene45666.t1 MTR_4g070080 90.244 82 8 0 4 85 2 83 1.27e-51 161
MS.gene45666.t1 MTR_4g070140 86.047 86 12 0 1 86 1 86 1.32e-50 159
MS.gene45666.t1 MTR_7g013730 46.154 91 45 1 1 91 37 123 1.13e-23 92.4
MS.gene45666.t1 MTR_3g075500 47.059 85 45 0 8 92 206 290 1.50e-23 92.8
MS.gene45666.t1 MTR_3g075500 40.741 81 48 0 9 89 110 190 8.24e-13 63.9
MS.gene45666.t1 MTR_4g109540 48.276 87 44 1 1 87 36 121 2.27e-23 92.0
MS.gene45666.t1 MTR_3g025880 44.706 85 47 0 3 87 39 123 5.54e-22 90.5
MS.gene45666.t1 MTR_4g109540 50.000 76 38 0 12 87 54 129 1.53e-21 87.4
MS.gene45666.t1 MTR_4g104470 46.914 81 43 0 8 88 216 296 1.10e-20 85.5
MS.gene45666.t1 MTR_4g104470 36.538 104 64 1 7 110 121 222 5.49e-16 72.8
MS.gene45666.t1 MTR_3g014000 44.860 107 41 4 1 100 31 126 1.87e-20 81.3
MS.gene45666.t1 MTR_1g064230 47.674 86 45 0 3 88 191 276 2.81e-20 84.0
MS.gene45666.t1 MTR_1g064230 35.577 104 67 0 9 112 85 188 6.49e-11 58.5
MS.gene45666.t1 MTR_3g084040 48.544 103 52 1 9 111 37 138 2.66e-19 78.6
MS.gene45666.t1 MTR_3g075500 51.562 64 31 0 8 71 206 269 2.74e-19 81.3
MS.gene45666.t1 MTR_3g075500 40.741 81 48 0 9 89 110 190 6.15e-13 64.3
MS.gene45666.t1 MTR_3g080130 39.024 82 50 0 9 90 37 118 1.81e-18 76.3
MS.gene45666.t1 MTR_4g035310 40.171 117 58 4 5 111 12 126 2.06e-18 79.0
MS.gene45666.t1 MTR_4g035310 40.171 117 58 4 5 111 12 126 2.35e-18 78.6
MS.gene45666.t1 MTR_7g105260 37.500 96 57 1 6 98 30 125 3.50e-18 75.5
MS.gene45666.t1 MTR_1g045510 40.244 82 49 0 4 85 200 281 6.72e-18 77.8
MS.gene45666.t1 MTR_3g116130 42.105 95 50 3 5 95 25 118 9.39e-18 77.4
MS.gene45666.t1 MTR_3g092490 40.217 92 53 2 5 95 22 112 4.43e-17 73.6
MS.gene45666.t1 MTR_3g116130 42.105 95 50 3 5 95 25 118 4.61e-17 76.3
MS.gene45666.t1 MTR_3g092490 40.230 87 51 1 9 95 27 112 7.63e-17 74.3
MS.gene45666.t1 MTR_3g092490 40.230 87 51 1 9 95 27 112 7.76e-17 74.3
MS.gene45666.t1 MTR_6g477870 37.931 87 54 0 9 95 96 182 9.16e-17 72.8
MS.gene45666.t1 MTR_3g055990 41.772 79 44 1 9 87 7 83 1.02e-16 75.5
MS.gene45666.t1 MTR_3g055990 41.772 79 44 1 9 87 7 83 1.11e-16 75.5
MS.gene45666.t1 MTR_3g084040 42.466 73 36 1 9 81 37 103 1.88e-16 70.1
MS.gene45666.t1 MTR_5g064120 44.737 76 41 1 9 84 17 91 2.78e-16 73.6
MS.gene45666.t1 MTR_3g029580 44.595 74 41 0 9 82 33 106 3.73e-16 70.1
MS.gene45666.t1 MTR_8g024520 45.455 77 39 2 9 84 26 100 8.84e-16 72.0
MS.gene45666.t1 MTR_8g024520 45.455 77 39 2 9 84 26 100 9.02e-16 72.0
MS.gene45666.t1 MTR_7g117495 50.847 59 29 0 9 67 19 77 1.04e-15 71.6
MS.gene45666.t1 MTR_7g117495 50.847 59 29 0 9 67 19 77 1.27e-15 71.2
MS.gene45666.t1 MTR_7g099940 40.506 79 47 0 9 87 36 114 1.84e-15 68.6
MS.gene45666.t1 MTR_1g073690 35.443 79 49 1 9 87 7 83 3.17e-15 71.2
MS.gene45666.t1 MTR_1g073690 32.941 85 56 1 9 93 113 196 6.86e-12 62.0
MS.gene45666.t1 MTR_4g036415 34.884 129 80 2 10 137 453 578 5.44e-15 70.9
MS.gene45666.t1 MTR_5g097580 36.082 97 60 1 9 105 7 101 5.60e-15 70.9
MS.gene45666.t1 MTR_5g097580 32.184 87 58 1 9 95 107 192 7.39e-11 58.9
MS.gene45666.t1 MTR_5g097580 36.082 97 60 1 9 105 7 101 6.00e-15 70.5
MS.gene45666.t1 MTR_5g097580 32.184 87 58 1 9 95 107 192 7.94e-11 58.9
MS.gene45666.t1 MTR_7g013740 40.171 117 54 1 9 125 41 141 1.38e-14 66.6
MS.gene45666.t1 MTR_2g026180 36.709 79 48 1 9 87 5 81 1.63e-14 69.3
MS.gene45666.t1 MTR_3g029580 49.180 61 31 0 11 71 35 95 2.12e-14 65.9
MS.gene45666.t1 MTR_2g096560 35.366 82 53 0 9 90 8 89 1.28e-13 62.4
MS.gene45666.t1 MTR_4g081520 38.272 81 50 0 11 91 54 134 1.32e-13 63.9
MS.gene45666.t1 MTR_7g106440 36.667 90 57 0 4 93 208 297 1.47e-13 66.2
MS.gene45666.t1 MTR_3g082650 32.558 129 78 2 9 137 107 226 1.77e-13 66.2
MS.gene45666.t1 MTR_3g082650 42.857 77 35 1 9 85 19 86 3.29e-13 65.5
MS.gene45666.t1 MTR_6g004810 38.554 83 49 1 9 91 46 126 3.86e-13 65.5
MS.gene45666.t1 MTR_6g004810 31.068 103 70 1 9 111 134 235 1.34e-12 63.9
MS.gene45666.t1 MTR_4g117350 34.783 92 59 1 9 100 72 162 7.01e-13 62.4
MS.gene45666.t1 MTR_4g063560 38.356 73 45 0 9 81 34 106 1.11e-12 60.5
MS.gene45666.t1 MTR_4g113150 39.091 110 63 3 10 119 9 114 1.59e-12 63.5
MS.gene45666.t1 MTR_6g477870 39.683 63 38 0 9 71 96 158 1.68e-12 61.6
MS.gene45666.t1 MTR_4g113150 39.091 110 63 3 10 119 9 114 2.10e-12 63.2
MS.gene45666.t1 MTR_3g103420 35.714 98 57 2 9 102 11 106 2.12e-12 63.2
MS.gene45666.t1 MTR_5g017280 41.250 80 43 2 9 87 5 81 7.55e-12 61.6
MS.gene45666.t1 MTR_5g017280 41.250 80 43 2 9 87 5 81 8.49e-12 61.6
MS.gene45666.t1 MTR_3g065190 38.889 90 54 1 3 91 107 196 8.56e-12 61.6
MS.gene45666.t1 MTR_5g044970 38.554 83 51 0 10 92 12 94 2.69e-11 58.2
MS.gene45666.t1 MTR_7g088140 45.763 59 32 0 9 67 128 186 6.09e-11 58.9
MS.gene45666.t1 MTR_1g056520 32.099 81 55 0 4 84 203 283 9.26e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45666.t1 AT2G21660 84.706 85 13 0 1 85 1 85 2.83e-49 155
MS.gene45666.t1 AT2G21660 84.706 85 13 0 1 85 1 85 4.77e-49 155
MS.gene45666.t1 AT4G39260 78.889 90 19 0 4 93 2 91 5.82e-49 152
MS.gene45666.t1 AT4G39260 76.829 82 19 0 4 85 2 83 8.02e-44 140
MS.gene45666.t1 AT4G39260 76.829 82 19 0 4 85 2 83 2.94e-43 140
MS.gene45666.t1 AT3G26420 51.429 105 49 1 3 107 2 104 2.07e-31 112
MS.gene45666.t1 AT4G39260 59.259 108 18 1 4 111 2 83 2.27e-28 100
MS.gene45666.t1 AT4G13850 51.852 81 39 0 9 89 36 116 3.96e-25 93.2
MS.gene45666.t1 AT3G23830 50.602 83 41 0 9 91 36 118 4.79e-25 93.2
MS.gene45666.t1 AT3G23830 50.602 83 41 0 9 91 36 118 4.79e-25 93.2
MS.gene45666.t1 AT4G13850 50.602 83 41 0 9 91 36 118 5.18e-25 93.6
MS.gene45666.t1 AT4G13850 51.064 94 46 0 9 102 36 129 7.78e-24 90.1
MS.gene45666.t1 AT5G61030 48.101 79 41 0 9 87 41 119 1.02e-23 93.6
MS.gene45666.t1 AT5G61030 48.101 79 41 0 9 87 41 119 1.02e-23 93.6
MS.gene45666.t1 AT4G13850 51.064 94 46 0 9 102 36 129 1.11e-23 90.1
MS.gene45666.t1 AT5G50250 45.783 83 45 0 5 87 204 286 5.50e-22 89.0
MS.gene45666.t1 AT5G50250 38.462 104 62 1 7 110 112 213 8.76e-16 72.0
MS.gene45666.t1 AT2G21690 50.000 90 40 1 3 87 2 91 7.08e-22 84.3
MS.gene45666.t1 AT2G37220 44.444 81 45 0 9 89 205 285 8.01e-22 88.2
MS.gene45666.t1 AT2G37220 41.250 80 45 1 3 82 88 165 1.90e-11 60.1
MS.gene45666.t1 AT3G53460 44.578 83 46 0 9 91 258 340 8.03e-22 89.0
MS.gene45666.t1 AT3G53460 44.578 83 46 0 9 91 258 340 8.03e-22 89.0
MS.gene45666.t1 AT3G53460 44.578 83 46 0 9 91 258 340 8.03e-22 89.0
MS.gene45666.t1 AT3G53460 44.578 83 46 0 9 91 279 361 1.07e-21 89.0
MS.gene45666.t1 AT4G24770 43.902 82 46 0 8 89 244 325 1.22e-21 88.6
MS.gene45666.t1 AT4G24770 39.423 104 61 1 7 110 149 250 5.07e-16 73.2
MS.gene45666.t1 AT4G24770 43.902 82 46 0 8 89 244 325 1.22e-21 88.6
MS.gene45666.t1 AT4G24770 39.423 104 61 1 7 110 149 250 5.07e-16 73.2
MS.gene45666.t1 AT5G04280 46.465 99 52 1 9 107 8 105 1.35e-21 88.2
MS.gene45666.t1 AT1G74230 52.632 76 35 1 9 84 35 109 1.74e-20 84.7
MS.gene45666.t1 AT1G60650 38.095 126 73 2 5 125 9 134 5.67e-20 83.6
MS.gene45666.t1 AT1G60650 38.095 126 73 2 5 125 9 134 5.67e-20 83.6
MS.gene45666.t1 AT5G06210 41.573 89 52 0 6 94 32 120 1.35e-19 79.3
MS.gene45666.t1 AT1G76460 48.684 76 38 1 9 84 25 99 1.16e-18 79.7
MS.gene45666.t1 AT1G20880 47.368 76 39 1 9 84 25 99 1.42e-18 79.3
MS.gene45666.t1 AT1G20880 47.368 76 39 1 9 84 25 99 1.42e-18 79.3
MS.gene45666.t1 AT1G20880 47.368 76 39 1 9 84 25 99 1.42e-18 79.3
MS.gene45666.t1 AT1G22330 42.308 104 58 2 9 112 18 119 4.74e-18 78.2
MS.gene45666.t1 AT1G18630 44.872 78 43 0 9 86 37 114 6.04e-18 75.5
MS.gene45666.t1 AT5G55550 36.792 106 65 1 9 114 7 110 2.78e-17 77.4
MS.gene45666.t1 AT5G55550 36.792 106 65 1 9 114 7 110 2.78e-17 77.4
MS.gene45666.t1 AT5G55550 36.792 106 65 1 9 114 7 110 2.78e-17 77.4
MS.gene45666.t1 AT5G55550 36.792 106 65 1 9 114 7 110 2.78e-17 77.4
MS.gene45666.t1 AT5G55550 36.792 106 65 1 9 114 7 110 2.78e-17 77.4
MS.gene45666.t1 AT1G22910 40.404 99 54 3 5 99 9 106 2.99e-17 75.5
MS.gene45666.t1 AT5G55550 35.780 109 68 1 9 117 7 113 3.09e-17 77.0
MS.gene45666.t1 AT5G55550 35.780 109 68 1 9 117 7 113 3.09e-17 77.0
MS.gene45666.t1 AT5G55550 35.780 109 68 1 9 117 7 113 3.09e-17 77.0
MS.gene45666.t1 AT5G55550 35.780 109 68 1 9 117 7 113 3.09e-17 77.0
MS.gene45666.t1 AT1G22910 40.404 99 54 3 5 99 9 106 3.23e-17 75.5
MS.gene45666.t1 AT2G46780 43.421 76 42 1 9 84 23 97 7.11e-17 75.5
MS.gene45666.t1 AT2G46780 43.421 76 42 1 9 84 23 97 7.11e-17 75.5
MS.gene45666.t1 AT3G07810 41.772 79 44 1 9 87 7 83 1.46e-16 75.1
MS.gene45666.t1 AT3G07810 41.772 79 44 1 9 87 7 83 1.47e-16 75.1
MS.gene45666.t1 AT1G22910 40.404 99 54 3 5 99 9 106 1.93e-16 74.3
MS.gene45666.t1 AT4G26650 34.951 103 65 1 9 111 16 116 2.70e-16 74.3
MS.gene45666.t1 AT4G26650 34.951 103 65 1 9 111 13 113 2.74e-16 74.3
MS.gene45666.t1 AT1G60000 40.244 82 49 0 4 85 173 254 3.29e-16 72.8
MS.gene45666.t1 AT4G13860 41.892 74 43 0 9 82 4 77 4.97e-16 68.6
MS.gene45666.t1 AT1G78260 43.210 81 44 2 5 84 13 92 5.89e-16 72.8
MS.gene45666.t1 AT2G33410 34.831 89 56 1 9 97 7 93 7.60e-16 73.2
MS.gene45666.t1 AT2G33410 36.709 79 49 1 9 87 111 188 3.01e-11 60.1
MS.gene45666.t1 AT4G14300 37.079 89 54 1 9 97 7 93 9.68e-16 72.8
MS.gene45666.t1 AT4G14300 37.079 89 54 1 9 97 7 93 9.68e-16 72.8
MS.gene45666.t1 AT1G78260 43.210 81 44 2 5 84 13 92 1.22e-15 71.6
MS.gene45666.t1 AT1G33470 37.895 95 53 2 9 98 8 101 1.29e-15 71.2
MS.gene45666.t1 AT1G33470 37.895 95 53 2 9 98 8 101 1.29e-15 71.2
MS.gene45666.t1 AT1G33470 37.895 95 53 2 9 98 8 101 1.33e-15 71.2
MS.gene45666.t1 AT3G08000 41.667 84 49 0 9 92 42 125 1.52e-15 68.9
MS.gene45666.t1 AT5G59865 43.038 79 45 0 11 89 29 107 2.77e-15 67.4
MS.gene45666.t1 AT5G47320 41.558 77 45 0 9 85 32 108 3.02e-15 69.7
MS.gene45666.t1 AT3G13224 37.234 94 53 2 6 95 4 95 3.15e-15 71.2
MS.gene45666.t1 AT3G13224 31.429 105 68 2 3 106 94 195 2.89e-13 65.5
MS.gene45666.t1 AT5G19960 39.640 111 63 3 10 119 9 116 3.56e-15 70.9
MS.gene45666.t1 AT3G13224 37.931 87 52 1 9 95 20 104 4.11e-15 70.9
MS.gene45666.t1 AT3G13224 32.955 88 58 1 3 89 103 190 1.98e-13 66.2
MS.gene45666.t1 AT3G54770 44.737 76 41 1 9 84 18 92 4.33e-15 70.1
MS.gene45666.t1 AT3G54770 44.737 76 41 1 9 84 18 92 4.56e-15 69.7
MS.gene45666.t1 AT2G37510 43.243 74 42 0 9 82 35 108 8.06e-15 67.0
MS.gene45666.t1 AT5G55550 43.662 71 38 1 9 79 7 75 1.04e-14 70.1
MS.gene45666.t1 AT5G55550 43.662 71 38 1 9 79 7 75 1.15e-14 69.7
MS.gene45666.t1 AT2G14160 40.698 86 32 2 1 68 1 85 1.56e-14 64.7
MS.gene45666.t1 AT3G46020 38.462 78 48 0 9 86 8 85 4.17e-14 63.9
MS.gene45666.t1 AT5G53720 41.250 80 46 1 5 84 6 84 4.82e-14 63.9
MS.gene45666.t1 AT1G01080 37.975 79 44 2 7 83 211 286 6.63e-14 67.0
MS.gene45666.t1 AT3G13224 37.931 87 52 1 9 95 20 104 6.67e-14 66.2
MS.gene45666.t1 AT1G01080 37.975 79 44 2 7 83 212 287 6.95e-14 67.0
MS.gene45666.t1 AT1G17640 40.000 80 46 1 9 88 67 144 8.12e-14 67.4
MS.gene45666.t1 AT1G17640 35.366 82 53 0 9 90 158 239 1.85e-11 60.5
MS.gene45666.t1 AT1G17640 40.000 80 46 1 9 88 67 144 8.12e-14 67.4
MS.gene45666.t1 AT1G17640 35.366 82 53 0 9 90 158 239 1.85e-11 60.5
MS.gene45666.t1 AT1G17640 40.000 80 46 1 9 88 67 144 8.12e-14 67.4
MS.gene45666.t1 AT1G17640 35.366 82 53 0 9 90 158 239 1.85e-11 60.5
MS.gene45666.t1 AT3G52150 39.759 83 50 0 2 84 171 253 1.00e-13 66.2
MS.gene45666.t1 AT3G52150 39.759 83 50 0 2 84 171 253 1.00e-13 66.2
MS.gene45666.t1 AT5G51300 30.400 125 86 1 7 131 479 602 3.60e-13 65.5
MS.gene45666.t1 AT5G51300 30.400 125 86 1 7 131 479 602 3.60e-13 65.5
MS.gene45666.t1 AT5G51300 30.400 125 86 1 7 131 479 602 3.60e-13 65.5
MS.gene45666.t1 AT5G53680 44.928 69 37 1 9 77 14 81 4.03e-13 63.2
MS.gene45666.t1 AT5G47620 35.443 79 49 1 9 87 7 83 4.50e-13 65.1
MS.gene45666.t1 AT5G47620 35.443 79 49 1 9 87 7 83 4.50e-13 65.1
MS.gene45666.t1 AT5G47620 35.443 79 49 1 9 87 7 83 4.50e-13 65.1
MS.gene45666.t1 AT2G27330 26.667 90 66 0 10 99 23 112 4.91e-13 61.6
MS.gene45666.t1 AT5G40490 43.210 81 44 1 9 89 43 121 5.22e-13 65.1
MS.gene45666.t1 AT5G40490 31.522 92 62 1 1 91 122 213 6.23e-11 58.9
MS.gene45666.t1 AT1G73530 35.000 80 52 0 9 88 78 157 5.52e-13 63.2
MS.gene45666.t1 AT5G54580 43.590 78 44 0 10 87 58 135 5.72e-13 62.4
MS.gene45666.t1 AT5G11412 43.373 83 45 2 3 84 46 127 5.83e-13 62.8
MS.gene45666.t1 AT4G13860 44.068 59 33 0 24 82 2 60 3.70e-12 58.2
MS.gene45666.t1 AT5G47620 47.059 51 27 0 9 59 7 57 3.99e-12 62.4
MS.gene45666.t1 AT3G52380 35.714 84 54 0 4 87 215 298 5.50e-12 62.0
MS.gene45666.t1 AT3G52380 37.838 74 46 0 9 82 117 190 2.03e-11 60.5
MS.gene45666.t1 AT3G06970 26.016 123 87 2 9 128 13 134 6.11e-12 61.6
MS.gene45666.t1 AT1G58470 40.541 74 39 2 8 77 6 78 1.69e-11 60.5
MS.gene45666.t1 AT1G58470 40.541 74 39 2 8 77 6 78 1.69e-11 60.5
MS.gene45666.t1 AT3G56860 41.270 63 37 0 5 67 137 199 2.17e-11 60.5
MS.gene45666.t1 AT3G56860 41.270 63 37 0 5 67 137 199 2.17e-11 60.5
MS.gene45666.t1 AT3G56860 41.270 63 37 0 5 67 137 199 2.17e-11 60.5
MS.gene45666.t1 AT3G56860 41.270 63 37 0 5 67 137 199 2.17e-11 60.5
MS.gene45666.t1 AT3G56860 41.270 63 37 0 5 67 137 199 2.17e-11 60.5
MS.gene45666.t1 AT3G56860 41.270 63 37 0 5 67 137 199 2.17e-11 60.5
MS.gene45666.t1 AT3G56860 41.270 63 37 0 5 67 137 199 2.17e-11 60.5
MS.gene45666.t1 AT3G56860 41.270 63 37 0 5 67 137 199 2.17e-11 60.5
MS.gene45666.t1 AT3G56860 41.270 63 37 0 5 67 137 199 2.17e-11 60.5
MS.gene45666.t1 AT3G56860 41.270 63 37 0 5 67 137 199 2.17e-11 60.5
MS.gene45666.t1 AT3G56860 41.270 63 37 0 5 67 137 199 2.17e-11 60.5
MS.gene45666.t1 AT3G14100 37.179 78 49 0 7 84 143 220 2.22e-11 60.5
MS.gene45666.t1 AT2G41060 41.176 68 39 1 1 67 120 187 3.35e-11 60.1
MS.gene45666.t1 AT2G41060 41.176 68 39 1 1 67 120 187 3.35e-11 60.1
MS.gene45666.t1 AT2G41060 41.176 68 39 1 1 67 120 187 3.35e-11 60.1
MS.gene45666.t1 AT5G64200 37.179 78 49 0 8 85 16 93 3.52e-11 59.7
MS.gene45666.t1 AT5G64200 37.179 78 49 0 8 85 16 93 3.52e-11 59.7
MS.gene45666.t1 AT2G22100 39.506 81 48 1 10 90 165 244 3.71e-11 59.7
MS.gene45666.t1 AT3G47120 38.667 75 46 0 10 84 38 112 4.27e-11 59.3
MS.gene45666.t1 AT3G47120 39.189 74 45 0 11 84 39 112 4.90e-11 59.3
MS.gene45666.t1 AT1G54080 38.462 78 48 0 7 84 147 224 5.00e-11 59.3
MS.gene45666.t1 AT4G36960 41.270 63 37 0 6 68 1 63 5.56e-11 59.3
MS.gene45666.t1 AT4G36960 41.270 63 37 0 6 68 1 63 5.56e-11 59.3
MS.gene45666.t1 AT1G01080 36.765 68 38 2 7 72 211 275 7.96e-11 58.5

Find 59 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGAGGTGGTGGTGGATA+TGG 0.277157 4.4:-37760635 MS.gene45666:CDS
GGCCGTGGTGGCGGTGGATA+TGG 0.293898 4.4:-37760656 MS.gene45666:CDS
ACTGGAAGATCAAGAGGATT+TGG 0.294131 4.4:-37760803 MS.gene45666:CDS
GGTTATGGAGGAGGTGGTGG+TGG 0.326562 4.4:-37760560 MS.gene45666:CDS
GGTGGTTATGGAGGAGGTGG+TGG 0.328302 4.4:-37760563 MS.gene45666:CDS
AGTGGTGGTGGAGGTGGTTA+TGG 0.334178 4.4:-37760575 MS.gene45666:CDS
GGTGGATATGGTGGAGGTGG+TGG 0.349052 4.4:-37760644 MS.gene45666:CDS
ATTATCAACGATCGTGAAAC+TGG 0.354445 4.4:-37760821 MS.gene45666:CDS
GGAGGTGGTGGTGGATATGG+CGG 0.376984 4.4:-37760632 MS.gene45666:CDS
GGTGGAGGAGGCCGTGGTGG+CGG 0.386911 4.4:-37760665 MS.gene45666:CDS
GGATATGGTGGAGGTGGTGG+TGG 0.393513 4.4:-37760641 MS.gene45666:CDS
TATGGAGGAGGTGGTGGTGG+TGG 0.393935 4.4:-37760557 MS.gene45666:CDS
GGAGGAGGCCGTGGTGGCGG+TGG 0.397234 4.4:-37760662 MS.gene45666:CDS
CAGAGCCGTGGTAGCGGTGG+TGG 0.401834 4.4:-37760683 MS.gene45666:CDS
GGCGGTGGATATGGTGGAGG+TGG 0.414719 4.4:-37760647 MS.gene45666:CDS
GGTGGAGGTGGTTATGGAGG+AGG 0.420020 4.4:-37760569 MS.gene45666:CDS
GGAGGTGGTTATGGAGGAGG+TGG 0.426099 4.4:-37760566 MS.gene45666:CDS
AGCGGTGGTGGAGGAGGCCG+TGG 0.429688 4.4:-37760671 MS.gene45666:CDS
AGGGATGAACGGTTCAGATA+TGG 0.435664 4.4:-37760735 MS.gene45666:CDS
GCTTCGTCGGAGGTCTTGCA+TGG 0.446995 4.4:-37761224 MS.gene45666:CDS
GGTGGTGGTGGATATGGCGG+AGG 0.453193 4.4:-37760629 MS.gene45666:CDS
GGTGGTGGATATGGCGGAGG+TGG 0.460996 4.4:-37760626 MS.gene45666:CDS
GGTGGTGGAGGTGGTTATGG+AGG 0.464167 4.4:-37760572 MS.gene45666:CDS
CGTGGTAGCGGTGGTGGAGG+AGG 0.477687 4.4:-37760677 MS.gene45666:CDS
GGTGGTGGTGGTTATGGTGG+TGG 0.479742 4.4:-37760545 None:intergenic
AGCCGTGGTAGCGGTGGTGG+AGG 0.481554 4.4:-37760680 MS.gene45666:CDS
GTTGAGTACCGTTGCTTCGT+CGG 0.485717 4.4:-37761237 MS.gene45666:CDS
AGAAGCAGTGGTGGTGGAGG+TGG 0.500860 4.4:-37760581 MS.gene45666:CDS
TCGATGAGAGATGCGATTGA+AGG 0.506178 4.4:-37760755 MS.gene45666:CDS
GGCGGAGGTGGTCGCCGCGA+GGG 0.508855 4.4:-37760614 MS.gene45666:CDS
AGCAGAAGCAGTGGTGGTGG+AGG 0.523650 4.4:-37760584 MS.gene45666:CDS
GAGGCTCAGAGCCGTGGTAG+CGG 0.524200 4.4:-37760689 MS.gene45666:CDS
GAGAAAGCCTTCTCTCAATA+CGG 0.531059 4.4:-37761180 MS.gene45666:CDS
CGATGAGAGATGCGATTGAA+GGG 0.538221 4.4:-37760754 MS.gene45666:CDS
GGAGGTGGTCGCCGCGAGGG+TGG 0.545142 4.4:-37760611 MS.gene45666:CDS
TACAGCAGAAGCAGTGGTGG+TGG 0.545974 4.4:-37760587 MS.gene45666:CDS
CTTCGTCGGAGGTCTTGCAT+GGG 0.553297 4.4:-37761223 MS.gene45666:CDS
GGCTACAGCAGAAGCAGTGG+TGG 0.557451 4.4:-37760590 MS.gene45666:CDS
CGTGGTGGCGGTGGATATGG+TGG 0.562612 4.4:-37760653 MS.gene45666:CDS
CGGAGAAATCATCGATTCGA+AGG 0.564623 4.4:-37761160 MS.gene45666:intron
CTCGAGAGCTTCGCTATCGG+TGG 0.564705 4.4:+37761200 None:intergenic
GCTCAGAGCCGTGGTAGCGG+TGG 0.565666 4.4:-37760686 MS.gene45666:CDS
GATTTCTCCGTATTGAGAGA+AGG 0.569467 4.4:+37761173 None:intergenic
CACCATATCCACCGCCACCA+CGG 0.571564 4.4:+37760654 None:intergenic
CGTGAAACTGGAAGATCAAG+AGG 0.575579 4.4:-37760809 MS.gene45666:CDS
GGTGGCGGTGGATATGGTGG+AGG 0.578322 4.4:-37760650 MS.gene45666:CDS
TTTCTCGAGAGCTTCGCTAT+CGG 0.583736 4.4:+37761197 None:intergenic
AAGGAACATTACTGTGAATG+AGG 0.589832 4.4:-37760708 MS.gene45666:CDS
CTCCTCCACCACCGCTACCA+CGG 0.595147 4.4:+37760678 None:intergenic
GGTGGTGGAGGAGGCCGTGG+TGG 0.601880 4.4:-37760668 MS.gene45666:CDS
CAAGACCTCCGACGAAGCAA+CGG 0.604652 4.4:+37761229 None:intergenic
GGTGGCTACAGCAGAAGCAG+TGG 0.609371 4.4:-37760593 MS.gene45666:CDS
GAGTACCGTTGCTTCGTCGG+AGG 0.615538 4.4:-37761234 MS.gene45666:CDS
TGGCGGAGGTGGTCGCCGCG+AGG 0.618580 4.4:-37760615 MS.gene45666:CDS
GTGAATGAGGCTCAGAGCCG+TGG 0.629363 4.4:-37760695 MS.gene45666:CDS
GATGCGATTGAAGGGATGAA+CGG 0.651599 4.4:-37760746 MS.gene45666:CDS
ACGGTTCAGATATGGATGGA+AGG 0.652700 4.4:-37760727 MS.gene45666:CDS
ATGAACGGTTCAGATATGGA+TGG 0.654412 4.4:-37760731 MS.gene45666:CDS
TTCTGCTGTAGCCACCCTCG+CGG 0.713605 4.4:+37760600 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGATCGATCGGTTACTATA+TGG - chr4.4:37760890-37760909 MS.gene45666:intron 35.0%
AAGGAACATTACTGTGAATG+AGG - chr4.4:37761090-37761109 MS.gene45666:intron 35.0%
ACCAAAAGAAGAAGATCGAT+CGG - chr4.4:37760879-37760898 MS.gene45666:intron 35.0%
AGATAGATCACAGATCAGAA+AGG + chr4.4:37760731-37760750 None:intergenic 35.0%
ATGAAGATGAACGTACCTAT+TGG + chr4.4:37760853-37760872 None:intergenic 35.0%
ATTATCAACGATCGTGAAAC+TGG - chr4.4:37760977-37760996 MS.gene45666:intron 35.0%
TGAAGATGAACGTACCTATT+GGG + chr4.4:37760852-37760871 None:intergenic 35.0%
! ATTTTGTTACTCACCCCAAT+AGG - chr4.4:37760835-37760854 MS.gene45666:intron 35.0%
!!! GATCTGACACGATTTTGATT+CGG - chr4.4:37760692-37760711 MS.gene45666:CDS 35.0%
ACTGGAAGATCAAGAGGATT+TGG - chr4.4:37760995-37761014 MS.gene45666:intron 40.0%
ATGAACGGTTCAGATATGGA+TGG - chr4.4:37761067-37761086 MS.gene45666:intron 40.0%
CAGAGTAACACAGAACAGAT+CGG + chr4.4:37760754-37760773 None:intergenic 40.0%
GAAGATGAACGTACCTATTG+GGG + chr4.4:37760851-37760870 None:intergenic 40.0%
GAGAAAGCCTTCTCTCAATA+CGG - chr4.4:37760618-37760637 MS.gene45666:CDS 40.0%
GATTTCTCCGTATTGAGAGA+AGG + chr4.4:37760628-37760647 None:intergenic 40.0%
! ACCGATCGATCTTCTTCTTT+TGG + chr4.4:37760883-37760902 None:intergenic 40.0%
ACGGTTCAGATATGGATGGA+AGG - chr4.4:37761071-37761090 MS.gene45666:intron 45.0%
AGGGATGAACGGTTCAGATA+TGG - chr4.4:37761063-37761082 MS.gene45666:intron 45.0%
CGATGAGAGATGCGATTGAA+GGG - chr4.4:37761044-37761063 MS.gene45666:intron 45.0%
CGTGAAACTGGAAGATCAAG+AGG - chr4.4:37760989-37761008 MS.gene45666:intron 45.0%
GATGCGATTGAAGGGATGAA+CGG - chr4.4:37761052-37761071 MS.gene45666:intron 45.0%
TCGATGAGAGATGCGATTGA+AGG - chr4.4:37761043-37761062 MS.gene45666:intron 45.0%
TTTCTCGAGAGCTTCGCTAT+CGG + chr4.4:37760604-37760623 None:intergenic 45.0%
!! CGGAGAAATCATCGATTCGA+AGG - chr4.4:37760638-37760657 MS.gene45666:CDS 45.0%
!! TTTGTGTTGCTTGCAGGCTT+CGG - chr4.4:37760665-37760684 MS.gene45666:CDS 45.0%
! GTTGAGTACCGTTGCTTCGT+CGG - chr4.4:37760561-37760580 MS.gene45666:CDS 50.0%
!! GGTCTGTTTGTGTTGCTTGC+AGG - chr4.4:37760659-37760678 MS.gene45666:CDS 50.0%
CAAGACCTCCGACGAAGCAA+CGG + chr4.4:37760572-37760591 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG - chr4.4:37760575-37760594 MS.gene45666:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG - chr4.4:37760670-37760689 MS.gene45666:CDS 55.0%
TACAGCAGAAGCAGTGGTGG+TGG - chr4.4:37761211-37761230 MS.gene45666:CDS 55.0%
!! AGTGGTGGTGGAGGTGGTTA+TGG - chr4.4:37761223-37761242 MS.gene45666:CDS 55.0%
CACCATATCCACCGCCACCA+CGG + chr4.4:37761147-37761166 None:intergenic 60.0%
CTCGAGAGCTTCGCTATCGG+TGG + chr4.4:37760601-37760620 None:intergenic 60.0%
GCTTCGTCGGAGGTCTTGCA+TGG - chr4.4:37760574-37760593 MS.gene45666:CDS 60.0%
GGAGGTGGTTATGGAGGAGG+TGG - chr4.4:37761232-37761251 MS.gene45666:CDS 60.0%
GGCTACAGCAGAAGCAGTGG+TGG - chr4.4:37761208-37761227 MS.gene45666:CDS 60.0%
GGTGGAGGTGGTTATGGAGG+AGG - chr4.4:37761229-37761248 MS.gene45666:CDS 60.0%
GGTGGCTACAGCAGAAGCAG+TGG - chr4.4:37761205-37761224 MS.gene45666:CDS 60.0%
GGTGGTTATGGAGGAGGTGG+TGG - chr4.4:37761235-37761254 MS.gene45666:CDS 60.0%
GTGAATGAGGCTCAGAGCCG+TGG - chr4.4:37761103-37761122 MS.gene45666:intron 60.0%
TTCTGCTGTAGCCACCCTCG+CGG + chr4.4:37761201-37761220 None:intergenic 60.0%
! GAGTACCGTTGCTTCGTCGG+AGG - chr4.4:37760564-37760583 MS.gene45666:CDS 60.0%
! GGATATGGTGGAGGTGGTGG+TGG - chr4.4:37761157-37761176 MS.gene45666:intron 60.0%
! GGTGGATATGGTGGAGGTGG+TGG - chr4.4:37761154-37761173 MS.gene45666:intron 60.0%
! GGTTATGGAGGAGGTGGTGG+TGG - chr4.4:37761238-37761257 MS.gene45666:CDS 60.0%
!! AGAAGCAGTGGTGGTGGAGG+TGG - chr4.4:37761217-37761236 MS.gene45666:CDS 60.0%
!! AGCAGAAGCAGTGGTGGTGG+AGG - chr4.4:37761214-37761233 MS.gene45666:CDS 60.0%
!! GGAGGTGGTGGTGGATATGG+CGG - chr4.4:37761166-37761185 MS.gene45666:CDS 60.0%
!! GGAGGTGGTGGTGGTGGTTA+TGG - chr4.4:37761247-37761266 MS.gene45666:CDS 60.0%
!! GGTGGAGGTGGTGGTGGATA+TGG - chr4.4:37761163-37761182 MS.gene45666:CDS 60.0%
!! GGTGGTGGAGGTGGTTATGG+AGG - chr4.4:37761226-37761245 MS.gene45666:CDS 60.0%
!! GGTGGTGGTGGTGGTTATGG+TGG - chr4.4:37761250-37761269 MS.gene45666:CDS 60.0%
!! TATGGAGGAGGTGGTGGTGG+TGG - chr4.4:37761241-37761260 MS.gene45666:CDS 60.0%
CTCCTCCACCACCGCTACCA+CGG + chr4.4:37761123-37761142 None:intergenic 65.0%
GAGGCTCAGAGCCGTGGTAG+CGG - chr4.4:37761109-37761128 MS.gene45666:intron 65.0%
! GGCGGTGGATATGGTGGAGG+TGG - chr4.4:37761151-37761170 MS.gene45666:intron 65.0%
! GGTGGCGGTGGATATGGTGG+AGG - chr4.4:37761148-37761167 MS.gene45666:intron 65.0%
!! CGTGGTGGCGGTGGATATGG+TGG - chr4.4:37761145-37761164 MS.gene45666:intron 65.0%
!! GGTGGTGGATATGGCGGAGG+TGG - chr4.4:37761172-37761191 MS.gene45666:CDS 65.0%
!! GGTGGTGGTGGATATGGCGG+AGG - chr4.4:37761169-37761188 MS.gene45666:CDS 65.0%
!! AGCCGTGGTAGCGGTGGTGG+AGG - chr4.4:37761118-37761137 MS.gene45666:intron 70.0%
!! CAGAGCCGTGGTAGCGGTGG+TGG - chr4.4:37761115-37761134 MS.gene45666:intron 70.0%
!! CGTGGTAGCGGTGGTGGAGG+AGG - chr4.4:37761121-37761140 MS.gene45666:intron 70.0%
!! GCTCAGAGCCGTGGTAGCGG+TGG - chr4.4:37761112-37761131 MS.gene45666:intron 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG - chr4.4:37761142-37761161 MS.gene45666:intron 70.0%
GGTGGAGGAGGCCGTGGTGG+CGG - chr4.4:37761133-37761152 MS.gene45666:intron 75.0%
!! AGCGGTGGTGGAGGAGGCCG+TGG - chr4.4:37761127-37761146 MS.gene45666:intron 75.0%
!! GGTGGTGGAGGAGGCCGTGG+TGG - chr4.4:37761130-37761149 MS.gene45666:intron 75.0%
! GGAGGTGGTCGCCGCGAGGG+TGG - chr4.4:37761187-37761206 MS.gene45666:CDS 80.0%
! GGCGGAGGTGGTCGCCGCGA+GGG - chr4.4:37761184-37761203 MS.gene45666:CDS 80.0%
! TGGCGGAGGTGGTCGCCGCG+AGG - chr4.4:37761183-37761202 MS.gene45666:CDS 80.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG - chr4.4:37761136-37761155 MS.gene45666:intron 80.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 37760546 37761274 37760546 ID=MS.gene45666
chr4.4 mRNA 37760546 37761274 37760546 ID=MS.gene45666.t1;Parent=MS.gene45666
chr4.4 exon 37761161 37761274 37761161 ID=MS.gene45666.t1.exon1;Parent=MS.gene45666.t1
chr4.4 CDS 37761161 37761274 37761161 ID=cds.MS.gene45666.t1;Parent=MS.gene45666.t1
chr4.4 exon 37760546 37760843 37760546 ID=MS.gene45666.t1.exon2;Parent=MS.gene45666.t1
chr4.4 CDS 37760546 37760843 37760546 ID=cds.MS.gene45666.t1;Parent=MS.gene45666.t1
Gene Sequence

>MS.gene45666

ATGGCTTCTGCAGATGTTGAGTACCGTTGCTTCGTCGGAGGTCTTGCATGGGCCACCGATAGCGAAGCTCTCGAGAAAGCCTTCTCTCAATACGGAGAAATCATCGATTCGAAGGTCTGTTTGTGTTGCTTGCAGGCTTCGGATCGGATCTGACACGATTTTGATTCGGATTTCTCATGAAACCTTTCTGATCTGTGATCTATCTCCGATCTGTTCTGTGTTACTCTGTTACTTGATCTCTATTATTCTGTTACTACTGTTACTGTGATTGATTCTGTTACTATATGATATTTTGTTACTCACCCCAATAGGTACGTTCATCTTCATCTTTTAACCAAAAGAAGAAGATCGATCGGTTACTATATGGTTTTTGTTTTGTTTTTTAGTGTTAAATTTGTAGATCTGATTTTGTGTTGTTGATTTTTGTGTAGATTATCAACGATCGTGAAACTGGAAGATCAAGAGGATTTGGATTTGTGACATTTGCTGATGAGAAATCGATGAGAGATGCGATTGAAGGGATGAACGGTTCAGATATGGATGGAAGGAACATTACTGTGAATGAGGCTCAGAGCCGTGGTAGCGGTGGTGGAGGAGGCCGTGGTGGCGGTGGATATGGTGGAGGTGGTGGTGGATATGGCGGAGGTGGTCGCCGCGAGGGTGGCTACAGCAGAAGCAGTGGTGGTGGAGGTGGTTATGGAGGAGGTGGTGGTGGTGGTTATGGTGGTG
Protein sequence

>MS.gene45666.t1

MASADVEYRCFVGGLAWATDSEALEKAFSQYGEIIDSKIINDRETGRSRGFGFVTFADEKSMRDAIEGMNGSDMDGRNITVNEAQSRGSGGGGGRGGGGYGGGGGGYGGGGRREGGYSRSSGGGGGYGGGGGGGYGG