AlfalfaGEDB | MS.gene45814

M. sativa cultivar XinJiangDaYe / MS.gene45814

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45814.t1	XP_013447003.1	96.2	78	3	0	2	79	1215	1292	1.60E-34	154.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45814.t1	A0A072U5A2	96.2	78	3	0	2	79	1215	1292	1.2e-34	154.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene45814	0.818344	2.06E-52	-1.69E-46
MS.gene052519	MS.gene45814	0.80454	2.06E-49	-1.69E-46
MS.gene054236	MS.gene45814	0.817331	3.49E-52	-1.69E-46
MS.gene05458	MS.gene45814	0.809231	2.10E-50	-1.69E-46
MS.gene054651	MS.gene45814	0.818055	2.39E-52	-1.69E-46
MS.gene054775	MS.gene45814	0.81615	6.42E-52	-1.69E-46
MS.gene055228	MS.gene45814	0.800508	1.39E-48	-1.69E-46
MS.gene056050	MS.gene45814	0.806356	8.56E-50	-1.69E-46
MS.gene058010	MS.gene45814	0.802712	4.93E-49	-1.69E-46
MS.gene060011	MS.gene45814	0.800597	1.34E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45814.t1	MTR_8g096930	96.154	78	3	0	2	79	1215	1292	1.07e-34	124

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 25 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGCATTTAAGACTCTTAC+AGG	0.232400	4.4:+76527503	MS.gene45814:CDS
GTATCAGCGTTGCCAGATTT+TGG	0.244275	4.4:+76527605	MS.gene45814:CDS
GACCCATGTTTATGAATTCT+AGG	0.330788	4.4:+76527462	None:intergenic
CTCATTCATGACTGCTGCCC+TGG	0.344555	4.4:-76527689	None:intergenic
GAGGAAAATTTAGCAGTTCA+AGG	0.403757	4.4:+76527539	MS.gene45814:CDS
CCTCCATCATGACAGTTAAC+AGG	0.423982	4.4:-76527575	None:intergenic
CATGACAGTTAACAGGTTCC+TGG	0.424130	4.4:-76527568	None:intergenic
AAACTGGAAGATCCAAAATC+TGG	0.430033	4.4:-76527617	None:intergenic
ACAGGAGGCAAGTCCATGCC+AGG	0.447156	4.4:+76527671	MS.gene45814:CDS
TCATGACTGCTGCCCTGGCA+TGG	0.471120	4.4:-76527684	None:intergenic
TTCTCATTTCACTTTGACAC+AGG	0.502289	4.4:+76527653	MS.gene45814:CDS
GGAAAATTTAGCAGTTCAAG+GGG	0.517942	4.4:+76527541	MS.gene45814:CDS
TTAAATGCAAATTCTAAGCA+TGG	0.532654	4.4:-76527491	None:intergenic
AGGAAAATTTAGCAGTTCAA+GGG	0.541169	4.4:+76527540	MS.gene45814:CDS
GAACCTGTTAACTGTCATGA+TGG	0.554557	4.4:+76527572	MS.gene45814:CDS
CTGTTAACTGTCATGATGGA+GGG	0.557709	4.4:+76527576	MS.gene45814:CDS
AACTGTCATGATGGAGGGGA+TGG	0.576525	4.4:+76527581	MS.gene45814:CDS
TGACCTAGAATTCATAAACA+TGG	0.584462	4.4:-76527465	None:intergenic
TACAGGTGTGATTCCAGTAG+AGG	0.585100	4.4:+76527520	MS.gene45814:CDS
TCATTTCACTTTGACACAGG+AGG	0.596195	4.4:+76527656	MS.gene45814:CDS
GACCTAGAATTCATAAACAT+GGG	0.603254	4.4:-76527464	None:intergenic
CCTGTTAACTGTCATGATGG+AGG	0.606160	4.4:+76527575	MS.gene45814:CDS
CAGGAGGCAAGTCCATGCCA+GGG	0.650398	4.4:+76527672	MS.gene45814:CDS
TGTTAACTGTCATGATGGAG+GGG	0.666653	4.4:+76527577	MS.gene45814:CDS
GAAAATTTAGCAGTTCAAGG+GGG	0.735832	4.4:+76527542	MS.gene45814:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTAAATGCAAATTCTAAGCA+TGG	-	chr4.4:76527494-76527513	None:intergenic	25.0%
	AGGAAAATTTAGCAGTTCAA+GGG	+	chr4.4:76527540-76527559	MS.gene45814:CDS	30.0%
	GAAATCACTTTGTGAAAAAC+TGG	-	chr4.4:76527636-76527655	None:intergenic	30.0%
!	TTTGCATTTAAGACTCTTAC+AGG	+	chr4.4:76527503-76527522	MS.gene45814:CDS	30.0%
	AAACTGGAAGATCCAAAATC+TGG	-	chr4.4:76527620-76527639	None:intergenic	35.0%
	GAAAATTTAGCAGTTCAAGG+GGG	+	chr4.4:76527542-76527561	MS.gene45814:CDS	35.0%
	GAGGAAAATTTAGCAGTTCA+AGG	+	chr4.4:76527539-76527558	MS.gene45814:CDS	35.0%
	GGAAAATTTAGCAGTTCAAG+GGG	+	chr4.4:76527541-76527560	MS.gene45814:CDS	35.0%
	TTCTCATTTCACTTTGACAC+AGG	+	chr4.4:76527653-76527672	MS.gene45814:CDS	35.0%
!	ACTGCTAAATTTTCCTCTAC+TGG	-	chr4.4:76527536-76527555	None:intergenic	35.0%
	GAACCTGTTAACTGTCATGA+TGG	+	chr4.4:76527572-76527591	MS.gene45814:CDS	40.0%
	TCATTTCACTTTGACACAGG+AGG	+	chr4.4:76527656-76527675	MS.gene45814:CDS	40.0%
!	CTGTTAACTGTCATGATGGA+GGG	+	chr4.4:76527576-76527595	MS.gene45814:CDS	40.0%
!	TGTTAACTGTCATGATGGAG+GGG	+	chr4.4:76527577-76527596	MS.gene45814:CDS	40.0%
	CATGACAGTTAACAGGTTCC+TGG	-	chr4.4:76527571-76527590	None:intergenic	45.0%
	CCTCCATCATGACAGTTAAC+AGG	-	chr4.4:76527578-76527597	None:intergenic	45.0%
	CCTGTTAACTGTCATGATGG+AGG	+	chr4.4:76527575-76527594	MS.gene45814:CDS	45.0%
	TACAGGTGTGATTCCAGTAG+AGG	+	chr4.4:76527520-76527539	MS.gene45814:CDS	45.0%
!	GTATCAGCGTTGCCAGATTT+TGG	+	chr4.4:76527605-76527624	MS.gene45814:CDS	45.0%
!	AACTGTCATGATGGAGGGGA+TGG	+	chr4.4:76527581-76527600	MS.gene45814:CDS	50.0%
	ACAGGAGGCAAGTCCATGCC+AGG	+	chr4.4:76527671-76527690	MS.gene45814:CDS	60.0%
	CAGGAGGCAAGTCCATGCCA+GGG	+	chr4.4:76527672-76527691	MS.gene45814:CDS	60.0%
	TCATGACTGCTGCCCTGGCA+TGG	-	chr4.4:76527687-76527706	None:intergenic	60.0%
!!	AGCAGTTCAAGGGGGTGTCC+AGG	+	chr4.4:76527550-76527569	MS.gene45814:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	76527467	76527706	76527467	ID=MS.gene45814
chr4.4	mRNA	76527467	76527706	76527467	ID=MS.gene45814.t1;Parent=MS.gene45814
chr4.4	exon	76527467	76527706	76527467	ID=MS.gene45814.t1.exon1;Parent=MS.gene45814.t1
chr4.4	CDS	76527467	76527706	76527467	ID=cds.MS.gene45814.t1;Parent=MS.gene45814.t1

Gene Sequence	>MS.gene45814 ATGTTTATGAATTCTAGGTCAGAACCATGCTTAGAATTTGCATTTAAGACTCTTACAGGTGTGATTCCAGTAGAGGAAAATTTAGCAGTTCAAGGGGGTGTCCAGGAACCTGTTAACTGTCATGATGGAGGGGATGGTGTATCAGCGTTGCCAGATTTTGGATCTTCCAGTTTTTCACAAAGTGATTTCTCATTTCACTTTGACACAGGAGGCAAGTCCATGCCAGGGCAGCAGTCATGA
Protein sequence	>MS.gene45814.t1 MFMNSRSEPCLEFAFKTLTGVIPVEENLAVQGGVQEPVNCHDGGDGVSALPDFGSSSFSQSDFSFHFDTGGKSMPGQQS