AlfalfaGEDB | MS.gene46230

M. sativa cultivar XinJiangDaYe / MS.gene46230

KEGG: K14686
GO: GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016021 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0034220 GO:0035434 GO:0046873 GO:0046915 GO:0051179 GO:0051234 GO:0055085 GO:0098655 GO:0098660 GO:0098662 GO:0110165
PFAM: PF04145.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46230.t1	XP_003606781.2	98.7	153	2	0	1	153	1	153	5.50E-79	303.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46230.t1	Q8GWP3	53.4	146	55	5	5	144	1	139	9.0e-30	131.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46230.t1	G7JDP2	98.7	153	2	0	1	153	1	153	3.9e-79	303.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051158	MS.gene46230	0.801397	9.18E-49	-1.69E-46
MS.gene055595	MS.gene46230	0.971184	9.14E-133	-1.69E-46
MS.gene055597	MS.gene46230	0.963806	1.54E-122	-1.69E-46
MS.gene056330	MS.gene46230	0.805691	1.18E-49	-1.69E-46
MS.gene056899	MS.gene46230	0.807515	4.87E-50	-1.69E-46
MS.gene059043	MS.gene46230	0.806599	7.61E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46230.t1	MTR_4g065660	98.693	153	2	0	1	153	1	153	3.36e-110	310
MS.gene46230.t1	MTR_4g064963	81.667	120	21	1	35	153	46	165	1.23e-64	195
MS.gene46230.t1	MTR_4g065123	74.074	135	33	2	9	142	4	137	7.33e-58	177
MS.gene46230.t1	MTR_7g066070	46.154	143	69	4	8	147	2	139	1.61e-36	123
MS.gene46230.t1	MTR_4g019870	46.087	115	61	1	32	145	13	127	1.48e-33	115
MS.gene46230.t1	MTR_0027s0220	51.282	117	54	2	17	130	12	128	6.52e-32	111
MS.gene46230.t1	MTR_1g015000	38.583	127	57	5	31	136	1	127	1.15e-18	77.4
MS.gene46230.t1	MTR_3g105330	35.156	128	59	3	31	134	1	128	1.43e-15	69.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46230.t1	AT2G26975	53.061	147	56	5	5	145	1	140	6.48e-42	137
MS.gene46230.t1	AT5G59030	45.833	168	76	5	1	153	1	168	1.47e-36	124
MS.gene46230.t1	AT3G46900	49.587	121	57	3	36	152	34	154	4.16e-34	117
MS.gene46230.t1	AT5G59040	45.082	122	66	1	30	150	28	149	4.57e-31	109
MS.gene46230.t1	AT2G37925	43.860	114	60	2	30	139	28	141	3.57e-27	99.4
MS.gene46230.t1	AT5G20650	38.462	130	54	4	31	134	1	130	2.36e-16	71.6

Find 43 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGGGTTGGCCTAGCTTATT+TGG	0.200727	4.3:-38720240	MS.gene46230:CDS
TTTCAATGGTGGGGTGTTCT+TGG	0.291991	4.3:-38720198	MS.gene46230:CDS
CATTGATTCTCTTTGACAAT+TGG	0.313757	4.3:-38720412	MS.gene46230:CDS
TGATGCACATGACATTCTTC+TGG	0.316430	4.3:-38720445	MS.gene46230:CDS
CTGTGCTTGGTCATGCTGTT+GGG	0.361371	4.3:-38720172	MS.gene46230:CDS
CTCTTTGACAATTGGCCTGC+TGG	0.377320	4.3:-38720404	MS.gene46230:CDS
TGAACTAGACCGGCCACAAC+AGG	0.384941	4.3:+38720281	None:intergenic
GGCCACAACAGGGTTTGAAC+CGG	0.386419	4.3:+38720292	None:intergenic
GCTGTGCTTGGTCATGCTGT+TGG	0.390679	4.3:-38720173	MS.gene46230:CDS
AATTGGCCTGCTGGTAGTTC+TGG	0.393131	4.3:-38720395	MS.gene46230:CDS
CACTCATGTCTTTCAATGGT+GGG	0.393883	4.3:-38720208	MS.gene46230:CDS
TGAGAGAAACTCGATGAGTA+TGG	0.396012	4.3:+38720334	None:intergenic
CCTAGCTTATTTGGTTATGT+TGG	0.401335	4.3:-38720231	MS.gene46230:CDS
TTGAACCGGGTTTAATGAAC+CGG	0.410106	4.3:+38720306	None:intergenic
GTGTTCTTGGTTGCTGTGCT+TGG	0.410298	4.3:-38720185	MS.gene46230:CDS
ACCCATCATCATAGTGTCAT+TGG	0.428445	4.3:+38720481	None:intergenic
TACCAATGACACTATGATGA+TGG	0.457064	4.3:-38720483	MS.gene46230:CDS
TAGTTCTGGTAAGTATGTAT+TGG	0.476161	4.3:-38720381	MS.gene46230:CDS
GATGCACATGACATTCTTCT+GGG	0.480501	4.3:-38720444	MS.gene46230:CDS
GCACTCATGTCTTTCAATGG+TGG	0.488475	4.3:-38720209	MS.gene46230:CDS
GCCACAACAGGGTTTGAACC+GGG	0.512994	4.3:+38720293	None:intergenic
TTGGCACTCATGTCTTTCAA+TGG	0.515628	4.3:-38720212	MS.gene46230:CDS
GGTTCAAACCCTGTTGTGGC+CGG	0.535871	4.3:-38720290	MS.gene46230:CDS
TTGGTAGATGATGATGACTT+TGG	0.542496	4.3:+38720500	None:intergenic
ATGGATCATGACATGGATCA+TGG	0.546244	4.3:-38720536	MS.gene46230:CDS
CCAACATAACCAAATAAGCT+AGG	0.548358	4.3:+38720231	None:intergenic
TCGAAATTCTCATCTTGATG+TGG	0.556567	4.3:+38720119	None:intergenic
ACCCGGTTCAAACCCTGTTG+TGG	0.557595	4.3:-38720294	MS.gene46230:CDS
TCGAGTTTCTCTCAAGCACC+CGG	0.561462	4.3:-38720325	MS.gene46230:CDS
CAAAAGTGTCTGAACTAGAC+CGG	0.563621	4.3:+38720271	None:intergenic
ACCAATGACACTATGATGAT+GGG	0.572192	4.3:-38720482	MS.gene46230:CDS
AGCACCCGGTTCATTAAACC+CGG	0.579765	4.3:-38720311	MS.gene46230:CDS
ACTCATGTCTTTCAATGGTG+GGG	0.593261	4.3:-38720207	MS.gene46230:CDS
TGAACCGGGTTTAATGAACC+GGG	0.596910	4.3:+38720307	None:intergenic
TCATGACATGGATCATGGAA+TGG	0.630684	4.3:-38720531	MS.gene46230:CDS
AATAAGCTAGGCCAACCCTA+AGG	0.634400	4.3:+38720243	None:intergenic
TACTTACCAGAACTACCAGC+AGG	0.638786	4.3:+38720389	None:intergenic
CATGGATCATGGAATGGCAA+TGG	0.655000	4.3:-38720525	MS.gene46230:CDS
AAACTCGATGAGTATGGACA+TGG	0.667480	4.3:+38720340	None:intergenic
GAACTAGACCGGCCACAACA+GGG	0.667595	4.3:+38720282	None:intergenic
AAACAGCATGGATCATGACA+TGG	0.668548	4.3:-38720543	None:intergenic
AATAATTAACAAGATAACGG+AGG	0.670863	4.3:+38720092	None:intergenic
ATGCACATGACATTCTTCTG+GGG	0.703114	4.3:-38720443	MS.gene46230:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATTGATTCTCTTTGACAAT+TGG	-	chr4.3:38720221-38720240	MS.gene46230:CDS	30.0%
!!	TAGTTCTGGTAAGTATGTAT+TGG	-	chr4.3:38720252-38720271	MS.gene46230:CDS	30.0%
	ACCAATGACACTATGATGAT+GGG	-	chr4.3:38720151-38720170	MS.gene46230:CDS	35.0%
	CCAACATAACCAAATAAGCT+AGG	+	chr4.3:38720405-38720424	None:intergenic	35.0%
	CCTAGCTTATTTGGTTATGT+TGG	-	chr4.3:38720402-38720421	MS.gene46230:CDS	35.0%
	TACCAATGACACTATGATGA+TGG	-	chr4.3:38720150-38720169	MS.gene46230:CDS	35.0%
	TCGAAATTCTCATCTTGATG+TGG	+	chr4.3:38720517-38720536	None:intergenic	35.0%
!	TTGGTAGATGATGATGACTT+TGG	+	chr4.3:38720136-38720155	None:intergenic	35.0%
!!!	GATGTGGTTTTTTGAAAGCA+CGG	+	chr4.3:38720501-38720520	None:intergenic	35.0%
	AAACTCGATGAGTATGGACA+TGG	+	chr4.3:38720296-38720315	None:intergenic	40.0%
	ACCCATCATCATAGTGTCAT+TGG	+	chr4.3:38720155-38720174	None:intergenic	40.0%
	ACTCATGTCTTTCAATGGTG+GGG	-	chr4.3:38720426-38720445	MS.gene46230:CDS	40.0%
	ATGCACATGACATTCTTCTG+GGG	-	chr4.3:38720190-38720209	MS.gene46230:CDS	40.0%
	ATGGATCATGACATGGATCA+TGG	-	chr4.3:38720097-38720116	MS.gene46230:CDS	40.0%
	CACTCATGTCTTTCAATGGT+GGG	-	chr4.3:38720425-38720444	MS.gene46230:CDS	40.0%
	GATGCACATGACATTCTTCT+GGG	-	chr4.3:38720189-38720208	MS.gene46230:CDS	40.0%
	TCATGACATGGATCATGGAA+TGG	-	chr4.3:38720102-38720121	MS.gene46230:CDS	40.0%
	TGAGAGAAACTCGATGAGTA+TGG	+	chr4.3:38720302-38720321	None:intergenic	40.0%
	TGATGCACATGACATTCTTC+TGG	-	chr4.3:38720188-38720207	MS.gene46230:CDS	40.0%
	TTGAACCGGGTTTAATGAAC+CGG	+	chr4.3:38720330-38720349	None:intergenic	40.0%
	TTGGCACTCATGTCTTTCAA+TGG	-	chr4.3:38720421-38720440	MS.gene46230:CDS	40.0%
!	AGACACTTTTGCATGTCCTT+AGG	-	chr4.3:38720374-38720393	MS.gene46230:CDS	40.0%
!	GACACTTTTGCATGTCCTTA+GGG	-	chr4.3:38720375-38720394	MS.gene46230:CDS	40.0%
!!	CAAAAGTGTCTGAACTAGAC+CGG	+	chr4.3:38720365-38720384	None:intergenic	40.0%
	AATAAGCTAGGCCAACCCTA+AGG	+	chr4.3:38720393-38720412	None:intergenic	45.0%
	CATGGATCATGGAATGGCAA+TGG	-	chr4.3:38720108-38720127	MS.gene46230:CDS	45.0%
	GCACTCATGTCTTTCAATGG+TGG	-	chr4.3:38720424-38720443	MS.gene46230:CDS	45.0%
	TACTTACCAGAACTACCAGC+AGG	+	chr4.3:38720247-38720266	None:intergenic	45.0%
	TGAACCGGGTTTAATGAACC+GGG	+	chr4.3:38720329-38720348	None:intergenic	45.0%
!	CTTTTGCATGTCCTTAGGGT+TGG	-	chr4.3:38720379-38720398	MS.gene46230:CDS	45.0%
!	TTTCAATGGTGGGGTGTTCT+TGG	-	chr4.3:38720435-38720454	MS.gene46230:CDS	45.0%
!!	TAGGGTTGGCCTAGCTTATT+TGG	-	chr4.3:38720393-38720412	MS.gene46230:CDS	45.0%
	AGCACCCGGTTCATTAAACC+CGG	-	chr4.3:38720322-38720341	MS.gene46230:CDS	50.0%
	CTGTGCTTGGTCATGCTGTT+GGG	-	chr4.3:38720461-38720480	MS.gene46230:CDS	50.0%
	GTGTTCTTGGTTGCTGTGCT+TGG	-	chr4.3:38720448-38720467	MS.gene46230:CDS	50.0%
	TCGAGTTTCTCTCAAGCACC+CGG	-	chr4.3:38720308-38720327	MS.gene46230:CDS	50.0%
!	AATTGGCCTGCTGGTAGTTC+TGG	-	chr4.3:38720238-38720257	MS.gene46230:CDS	50.0%
!	CTCTTTGACAATTGGCCTGC+TGG	-	chr4.3:38720229-38720248	MS.gene46230:CDS	50.0%
	ACCCGGTTCAAACCCTGTTG+TGG	-	chr4.3:38720339-38720358	MS.gene46230:CDS	55.0%
	GAACTAGACCGGCCACAACA+GGG	+	chr4.3:38720354-38720373	None:intergenic	55.0%
	GCCACAACAGGGTTTGAACC+GGG	+	chr4.3:38720343-38720362	None:intergenic	55.0%
	GCTGTGCTTGGTCATGCTGT+TGG	-	chr4.3:38720460-38720479	MS.gene46230:CDS	55.0%
	GGCCACAACAGGGTTTGAAC+CGG	+	chr4.3:38720344-38720363	None:intergenic	55.0%
	GGTTCAAACCCTGTTGTGGC+CGG	-	chr4.3:38720343-38720362	MS.gene46230:CDS	55.0%
	TGAACTAGACCGGCCACAAC+AGG	+	chr4.3:38720355-38720374	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	38720097	38720558	38720097	ID=MS.gene46230
chr4.3	mRNA	38720097	38720558	38720097	ID=MS.gene46230.t1;Parent=MS.gene46230
chr4.3	exon	38720097	38720558	38720097	ID=MS.gene46230.t1.exon1;Parent=MS.gene46230.t1
chr4.3	CDS	38720097	38720558	38720097	ID=cds.MS.gene46230.t1;Parent=MS.gene46230.t1

Gene Sequence

>MS.gene46230

ATGGATCATGACATGGATCATGGAATGGCAATGGCACCAAAGTCATCATCATCTACCAATGACACTATGATGATGGGTCAGATGCATGACATGATGCACATGACATTCTTCTGGGGTAAAGATGCATTGATTCTCTTTGACAATTGGCCTGCTGGTAGTTCTGGTAAGTATGTATTGGCTTTGATTCTTGTTTTCGCCATGTCCATACTCATCGAGTTTCTCTCAAGCACCCGGTTCATTAAACCCGGTTCAAACCCTGTTGTGGCCGGTCTAGTTCAGACACTTTTGCATGTCCTTAGGGTTGGCCTAGCTTATTTGGTTATGTTGGCACTCATGTCTTTCAATGGTGGGGTGTTCTTGGTTGCTGTGCTTGGTCATGCTGTTGGGTTCTTCTTTCGTAGCCGTGCTTTCAAAAAACCACATCAAGATGAGAATTTCGATCTTCCTCCGTTATCTTGTTAA

Protein sequence

>MS.gene46230.t1

MDHDMDHGMAMAPKSSSSTNDTMMMGQMHDMMHMTFFWGKDALILFDNWPAGSSGKYVLALILVFAMSILIEFLSSTRFIKPGSNPVVAGLVQTLLHVLRVGLAYLVMLALMSFNGGVFLVAVLGHAVGFFFRSRAFKKPHQDENFDLPPLSC