Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059043.t1 | XP_003594832.1 | 96.8 | 157 | 5 | 0 | 1 | 157 | 1 | 157 | 6.70E-80 | 306.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059043.t1 | P93333 | 88.5 | 157 | 18 | 0 | 1 | 157 | 1 | 157 | 1.6e-74 | 280.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059043.t1 | G7IMY8 | 96.8 | 157 | 5 | 0 | 1 | 157 | 1 | 157 | 4.8e-80 | 306.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049993 | MS.gene059043 | -0.804646 | 1.95E-49 | -1.69E-46 |
| MS.gene051158 | MS.gene059043 | 0.803534 | 3.33E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene059043 | 0.810481 | 1.13E-50 | -1.69E-46 |
| MS.gene052223 | MS.gene059043 | 0.813272 | 2.78E-51 | -1.69E-46 |
| MS.gene054183 | MS.gene059043 | -0.818947 | 1.50E-52 | -1.69E-46 |
| MS.gene055595 | MS.gene059043 | 0.807174 | 5.75E-50 | -1.69E-46 |
| MS.gene055601 | MS.gene059043 | 0.831339 | 1.77E-55 | -1.69E-46 |
| MS.gene056330 | MS.gene059043 | 0.817712 | 2.86E-52 | -1.69E-46 |
| MS.gene056690 | MS.gene059043 | 0.816113 | 6.54E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene059043 | 0.854827 | 9.50E-62 | -1.69E-46 |
| MS.gene056920 | MS.gene059043 | 0.804017 | 2.64E-49 | -1.69E-46 |
| MS.gene058218 | MS.gene059043 | 0.849281 | 3.58E-60 | -1.69E-46 |
| MS.gene059043 | MS.gene059294 | -0.824772 | 6.73E-54 | -1.69E-46 |
| MS.gene059043 | MS.gene059420 | -0.812702 | 3.71E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene060087 | 0.807504 | 4.89E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene061659 | -0.803807 | 2.92E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene062491 | 0.810584 | 1.07E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene066937 | 0.835182 | 1.95E-56 | -1.69E-46 |
| MS.gene059043 | MS.gene066938 | 0.829462 | 5.08E-55 | -1.69E-46 |
| MS.gene059043 | MS.gene069486 | 0.811033 | 8.57E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene069487 | 0.824745 | 6.84E-54 | -1.69E-46 |
| MS.gene059043 | MS.gene070257 | 0.831403 | 1.70E-55 | -1.69E-46 |
| MS.gene059043 | MS.gene070491 | -0.811368 | 7.25E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene070835 | -0.832359 | 9.89E-56 | -1.69E-46 |
| MS.gene059043 | MS.gene071108 | 0.800839 | 1.19E-48 | -1.69E-46 |
| MS.gene059043 | MS.gene071837 | 0.817706 | 2.87E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene073286 | 0.837968 | 3.80E-57 | -1.69E-46 |
| MS.gene059043 | MS.gene073526 | 0.80227 | 6.07E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene08264 | -0.805401 | 1.36E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene09768 | 0.841245 | 5.34E-58 | -1.69E-46 |
| MS.gene059043 | MS.gene20556 | -0.822528 | 2.26E-53 | -1.69E-46 |
| MS.gene059043 | MS.gene22386 | -0.808558 | 2.92E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene23016 | -0.804246 | 2.37E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene23869 | 0.809224 | 2.10E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene25652 | 0.810416 | 1.17E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene26289 | 0.841271 | 5.26E-58 | -1.69E-46 |
| MS.gene059043 | MS.gene28548 | 0.823259 | 1.53E-53 | -1.69E-46 |
| MS.gene059043 | MS.gene30518 | 0.832679 | 8.24E-56 | -1.69E-46 |
| MS.gene059043 | MS.gene30519 | 0.831171 | 1.94E-55 | -1.69E-46 |
| MS.gene059043 | MS.gene30520 | 0.847644 | 1.02E-59 | -1.69E-46 |
| MS.gene059043 | MS.gene30666 | 0.807068 | 6.05E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene31266 | -0.80094 | 1.14E-48 | -1.69E-46 |
| MS.gene059043 | MS.gene33198 | -0.803361 | 3.62E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene34874 | -0.803924 | 2.76E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene35433 | 0.833367 | 5.56E-56 | -1.69E-46 |
| MS.gene059043 | MS.gene35514 | -0.814707 | 1.34E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene35690 | 0.823185 | 1.59E-53 | -1.69E-46 |
| MS.gene059043 | MS.gene35721 | -0.821145 | 4.72E-53 | -1.69E-46 |
| MS.gene059043 | MS.gene37354 | 0.805903 | 1.07E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene37910 | -0.80173 | 7.85E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene38338 | 0.807195 | 5.69E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene40169 | -0.801279 | 9.71E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene42080 | 0.815751 | 7.87E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene42084 | 0.816265 | 6.05E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene42096 | 0.816449 | 5.50E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene43162 | 0.817334 | 3.48E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene43874 | 0.818492 | 1.91E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene44958 | 0.807451 | 5.02E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene45694 | 0.811711 | 6.11E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene45695 | 0.81589 | 7.33E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene46230 | 0.806599 | 7.61E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene46267 | 0.854924 | 8.90E-62 | -1.69E-46 |
| MS.gene059043 | MS.gene46272 | 0.8342 | 3.44E-56 | -1.69E-46 |
| MS.gene059043 | MS.gene48169 | 0.812468 | 4.18E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene48182 | 0.820954 | 5.22E-53 | -1.69E-46 |
| MS.gene059043 | MS.gene48184 | 0.831497 | 1.61E-55 | -1.69E-46 |
| MS.gene059043 | MS.gene48807 | 0.801744 | 7.79E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene50068 | 0.819445 | 1.16E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene50069 | 0.814112 | 1.82E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene50787 | 0.845882 | 3.08E-59 | -1.69E-46 |
| MS.gene059043 | MS.gene50790 | 0.824112 | 9.63E-54 | -1.69E-46 |
| MS.gene059043 | MS.gene52368 | 0.811366 | 7.26E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene52369 | 0.801826 | 7.50E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene54611 | 0.84999 | 2.27E-60 | -1.69E-46 |
| MS.gene059043 | MS.gene54630 | 0.812879 | 3.39E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene55270 | -0.80527 | 1.45E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene56622 | 0.80847 | 3.05E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene57807 | 0.80153 | 8.62E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene57813 | 0.809287 | 2.04E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene58270 | -0.800386 | 1.48E-48 | -1.69E-46 |
| MS.gene059043 | MS.gene58458 | 0.818186 | 2.23E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene60578 | 0.818666 | 1.74E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene60840 | 0.818479 | 1.92E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene60846 | 0.817363 | 3.43E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene60847 | 0.80425 | 2.36E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene62885 | 0.845441 | 4.05E-59 | -1.69E-46 |
| MS.gene059043 | MS.gene62886 | 0.846377 | 2.26E-59 | -1.69E-46 |
| MS.gene059043 | MS.gene63913 | 0.807371 | 5.22E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene65287 | 0.81183 | 5.75E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene66157 | -0.808696 | 2.73E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene67371 | 0.819596 | 1.07E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene67670 | 0.809783 | 1.59E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene70062 | 0.837246 | 5.82E-57 | -1.69E-46 |
| MS.gene059043 | MS.gene70064 | 0.832341 | 9.99E-56 | -1.69E-46 |
| MS.gene059043 | MS.gene71342 | 0.828333 | 9.53E-55 | -1.69E-46 |
| MS.gene059043 | MS.gene71346 | 0.805739 | 1.15E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene71395 | 0.82229 | 2.56E-53 | -1.69E-46 |
| MS.gene059043 | MS.gene71924 | 0.800779 | 1.23E-48 | -1.69E-46 |
| MS.gene059043 | MS.gene71927 | 0.840804 | 6.97E-58 | -1.69E-46 |
| MS.gene059043 | MS.gene72466 | -0.806855 | 6.72E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene72747 | -0.801132 | 1.04E-48 | -1.69E-46 |
| MS.gene059043 | MS.gene72924 | 0.847147 | 1.39E-59 | -1.69E-46 |
| MS.gene059043 | MS.gene74030 | 0.805368 | 1.38E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene74031 | 0.800491 | 1.41E-48 | -1.69E-46 |
| MS.gene059043 | MS.gene74032 | 0.80994 | 1.48E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene74553 | 0.82084 | 5.55E-53 | -1.69E-46 |
| MS.gene059043 | MS.gene75460 | 0.802479 | 5.50E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene80744 | 0.819392 | 1.19E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene81349 | 0.802941 | 4.42E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene81602 | -0.802906 | 4.49E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene81770 | 0.820617 | 6.24E-53 | -1.69E-46 |
| MS.gene059043 | MS.gene82245 | 0.804094 | 2.55E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene83008 | 0.811688 | 6.18E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene83011 | 0.805439 | 1.33E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene84373 | 0.815561 | 8.68E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene85276 | 0.816279 | 6.00E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene85750 | 0.812733 | 3.65E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene85860 | -0.803589 | 3.24E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene87043 | 0.811061 | 8.45E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene87055 | 0.830442 | 2.93E-55 | -1.69E-46 |
| MS.gene059043 | MS.gene87056 | 0.815878 | 7.38E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene87222 | 0.801951 | 7.07E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene87346 | 0.804552 | 2.05E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene87751 | 0.802514 | 5.41E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene89772 | 0.821428 | 4.06E-53 | -1.69E-46 |
| MS.gene059043 | MS.gene89773 | 0.83242 | 9.55E-56 | -1.69E-46 |
| MS.gene059043 | MS.gene89775 | 0.815551 | 8.72E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene89982 | 0.826759 | 2.27E-54 | -1.69E-46 |
| MS.gene059043 | MS.gene90814 | 0.803474 | 3.43E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene91221 | 0.845143 | 4.88E-59 | -1.69E-46 |
| MS.gene059043 | MS.gene91222 | 0.826371 | 2.81E-54 | -1.69E-46 |
| MS.gene059043 | MS.gene91509 | -0.813516 | 2.46E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene93236 | 0.816064 | 6.70E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene93632 | 0.8098 | 1.58E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene95353 | 0.814976 | 1.17E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene96244 | 0.80243 | 5.63E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene96371 | -0.805585 | 1.24E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene97335 | 0.822107 | 2.83E-53 | -1.69E-46 |
| MS.gene059043 | MS.gene98878 | 0.803238 | 3.83E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene99544 | 0.870685 | 1.21E-66 | -1.69E-46 |
| MS.gene059043 | MS.gene99545 | 0.823184 | 1.59E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059043.t1 | MTR_2g035130 | 96.815 | 157 | 5 | 0 | 1 | 157 | 1 | 157 | 2.36e-107 | 303 |
| MS.gene059043.t1 | MTR_2g035150 | 86.076 | 158 | 22 | 0 | 1 | 158 | 1 | 158 | 2.51e-97 | 278 |
| MS.gene059043.t1 | MTR_2g035100 | 88.535 | 157 | 18 | 0 | 1 | 157 | 1 | 157 | 5.64e-97 | 276 |
| MS.gene059043.t1 | MTR_2g035105 | 87.261 | 157 | 20 | 0 | 1 | 157 | 1 | 157 | 1.69e-95 | 273 |
| MS.gene059043.t1 | MTR_2g035120 | 86.076 | 158 | 22 | 0 | 1 | 158 | 1 | 158 | 3.27e-95 | 272 |
| MS.gene059043.t1 | MTR_6g033450 | 69.620 | 158 | 48 | 0 | 1 | 158 | 1 | 158 | 1.51e-78 | 230 |
| MS.gene059043.t1 | MTR_4g120970 | 69.620 | 158 | 48 | 0 | 1 | 158 | 1 | 158 | 1.51e-78 | 230 |
| MS.gene059043.t1 | MTR_2g035320 | 61.783 | 157 | 60 | 0 | 1 | 157 | 1 | 157 | 1.85e-65 | 197 |
| MS.gene059043.t1 | MTR_2g035190 | 59.873 | 157 | 63 | 0 | 1 | 157 | 1 | 157 | 2.58e-63 | 191 |
| MS.gene059043.t1 | MTR_2g035210 | 58.599 | 157 | 65 | 0 | 1 | 157 | 1 | 157 | 2.82e-61 | 186 |
| MS.gene059043.t1 | MTR_2g035220 | 58.599 | 157 | 65 | 0 | 1 | 157 | 1 | 157 | 4.58e-60 | 183 |
| MS.gene059043.t1 | MTR_2g035170 | 56.688 | 157 | 68 | 0 | 1 | 157 | 1 | 157 | 3.06e-58 | 179 |
| MS.gene059043.t1 | MTR_2g435310 | 37.821 | 156 | 96 | 1 | 1 | 155 | 1 | 156 | 1.46e-36 | 124 |
| MS.gene059043.t1 | MTR_4g120760 | 38.854 | 157 | 95 | 1 | 1 | 156 | 1 | 157 | 2.55e-36 | 123 |
| MS.gene059043.t1 | MTR_4g120760 | 38.854 | 157 | 95 | 1 | 1 | 156 | 1 | 157 | 3.13e-36 | 123 |
| MS.gene059043.t1 | MTR_2g035320 | 58.586 | 99 | 41 | 0 | 59 | 157 | 24 | 122 | 5.88e-36 | 121 |
| MS.gene059043.t1 | MTR_4g120950 | 45.062 | 162 | 82 | 5 | 1 | 158 | 1 | 159 | 1.21e-33 | 116 |
| MS.gene059043.t1 | MTR_4g120940 | 62.921 | 89 | 30 | 1 | 70 | 158 | 35 | 120 | 1.70e-32 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAGCATAGTTGGTGGTTT+TGG | 0.149266 | 2.4:-56029366 | MS.gene059043:CDS |
| TGATGGCATCAATAACCTTT+GGG | 0.301288 | 2.4:+56030269 | None:intergenic |
| AACGATATCAATACTCTTGA+TGG | 0.325047 | 2.4:+56030252 | None:intergenic |
| TTTGCAATGGTTCCTCCATT+TGG | 0.327211 | 2.4:+56029291 | None:intergenic |
| TATATGCTTTAGTTGTAATC+AGG | 0.341112 | 2.4:+56029150 | None:intergenic |
| TTGATGGCATCAATAACCTT+TGG | 0.345179 | 2.4:+56030268 | None:intergenic |
| GAGATCTTCTCCACTGTGTC+TGG | 0.364866 | 2.4:+56029339 | None:intergenic |
| GGCTCTTGAAGGTTACGTTT+TGG | 0.372797 | 2.4:-56029178 | MS.gene059043:CDS |
| CTTAGTAGATGATGTTAACT+TGG | 0.391360 | 2.4:-56029397 | MS.gene059043:CDS |
| TTCTTGAGTTCCTCTTCACT+TGG | 0.414096 | 2.4:+56029231 | None:intergenic |
| CATCAATAACCTTTGGGGTA+AGG | 0.433870 | 2.4:+56030275 | None:intergenic |
| GCTTATCACTACAGCATAGT+TGG | 0.443784 | 2.4:-56029375 | MS.gene059043:CDS |
| AAGAGTATTGATATCGTTGA+AGG | 0.465901 | 2.4:-56030247 | MS.gene059043:CDS |
| AAATTGTCTGCAGGACCAAA+TGG | 0.477800 | 2.4:-56029306 | MS.gene059043:CDS |
| AGAGCTTTGTAAAGTGTAGC+AGG | 0.480318 | 2.4:+56030316 | None:intergenic |
| AGTGTGAAATACTTCACAAA+AGG | 0.509404 | 2.4:-56029264 | MS.gene059043:CDS |
| AATGGCAAAGCTAGGGGTGA+TGG | 0.523022 | 2.4:-56029210 | MS.gene059043:CDS |
| TGCAGACAATTTAGCCTCAA+AGG | 0.525069 | 2.4:+56029317 | None:intergenic |
| TTTGAGGCTAAATTGTCTGC+AGG | 0.526963 | 2.4:-56029315 | MS.gene059043:CDS |
| AACTCAAGAATGGCAAAGCT+AGG | 0.540466 | 2.4:-56029218 | MS.gene059043:CDS |
| AAAGTATGTGTTGCACAAAG+TGG | 0.544046 | 2.4:-56029421 | MS.gene059043:CDS |
| ACTCAAGAATGGCAAAGCTA+GGG | 0.556431 | 2.4:-56029217 | MS.gene059043:CDS |
| GTTGAAGGAGACGGTGGCGC+CGG | 0.560015 | 2.4:-56030232 | MS.gene059043:CDS |
| TGTAGCAGGAGCTACAATAG+AGG | 0.565152 | 2.4:+56030330 | None:intergenic |
| TTTCACACTAAGCTTTGCAA+TGG | 0.568267 | 2.4:+56029278 | None:intergenic |
| TATCACTACAGCATAGTTGG+TGG | 0.572532 | 2.4:-56029372 | MS.gene059043:CDS |
| AGTGAAGAGGAACTCAAGAA+TGG | 0.578362 | 2.4:-56029228 | MS.gene059043:CDS |
| GATGGCATCAATAACCTTTG+GGG | 0.578696 | 2.4:+56030270 | None:intergenic |
| AGTGGAGAAGATCTCCTTTG+AGG | 0.588167 | 2.4:-56029331 | MS.gene059043:CDS |
| ATTGATATCGTTGAAGGAGA+CGG | 0.588878 | 2.4:-56030241 | MS.gene059043:CDS |
| TGATGCTGCACCAAGTGAAG+AGG | 0.611877 | 2.4:-56029241 | MS.gene059043:CDS |
| TGCTGACAACCTTACCCCAA+AGG | 0.621335 | 2.4:-56030284 | MS.gene059043:CDS |
| CTCAAGAATGGCAAAGCTAG+GGG | 0.641253 | 2.4:-56029216 | MS.gene059043:CDS |
| AGCAGGAGCTACAATAGAGG+TGG | 0.665165 | 2.4:+56030333 | None:intergenic |
| TTGTCTGCAGGACCAAATGG+AGG | 0.666387 | 2.4:-56029303 | MS.gene059043:CDS |
| GATATCGTTGAAGGAGACGG+TGG | 0.715509 | 2.4:-56030238 | MS.gene059043:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTATATATAAAATAAT+AGG | - | chr2.4:56030039-56030058 | MS.gene059043:intron | 0.0% |
| !! | TGATAAAAGAAATTGATAAA+CGG | + | chr2.4:56029568-56029587 | None:intergenic | 15.0% |
| !! | ACTCTTATGATTAACATATT+TGG | - | chr2.4:56029477-56029496 | MS.gene059043:intron | 20.0% |
| !! | ATCATTACTAGTATTACATA+AGG | - | chr2.4:56029999-56030018 | MS.gene059043:intron | 20.0% |
| !!! | ATATTTGGTAGCTAATTAAT+TGG | - | chr2.4:56029492-56029511 | MS.gene059043:intron | 20.0% |
| ! | AACTTACTTTAATACAACCT+TGG | + | chr2.4:56029689-56029708 | None:intergenic | 25.0% |
| ! | CTTTCAAATATACCGTTAAA+AGG | + | chr2.4:56029798-56029817 | None:intergenic | 25.0% |
| ! | GACTAAACTATACTTATTTC+CGG | + | chr2.4:56029874-56029893 | None:intergenic | 25.0% |
| ! | TAAAGAATGAGATCACTAAA+TGG | + | chr2.4:56029762-56029781 | None:intergenic | 25.0% |
| ! | TTAGTCAAAGACAAATTGTT+CGG | + | chr2.4:56029916-56029935 | None:intergenic | 25.0% |
| ! | TTTAGTGATCTCATTCTTTA+TGG | - | chr2.4:56029762-56029781 | MS.gene059043:intron | 25.0% |
| !! | AGAATGCTAGAATATTCTTT+TGG | - | chr2.4:56029816-56029835 | MS.gene059043:intron | 25.0% |
| !!! | TGATTTGGTATACTTGAAAA+AGG | - | chr2.4:56029637-56029656 | MS.gene059043:intron | 25.0% |
| AAAAGGTTCTTAGCACAAAA+TGG | - | chr2.4:56029654-56029673 | MS.gene059043:intron | 30.0% | |
| AACACAATTGCTTGATTAAC+TGG | - | chr2.4:56029953-56029972 | MS.gene059043:intron | 30.0% | |
| AACGATATCAATACTCTTGA+TGG | + | chr2.4:56029270-56029289 | None:intergenic | 30.0% | |
| AGTGTGAAATACTTCACAAA+AGG | - | chr2.4:56030255-56030274 | MS.gene059043:CDS | 30.0% | |
| CAAAATGGACAATTTAACCA+AGG | - | chr2.4:56029669-56029688 | MS.gene059043:intron | 30.0% | |
| CTTAGTAGATGATGTTAACT+TGG | - | chr2.4:56030122-56030141 | MS.gene059043:intron | 30.0% | |
| GATCTCATTCTTTATGGAAT+AGG | - | chr2.4:56029768-56029787 | MS.gene059043:intron | 30.0% | |
| TAGTCAAAGACAAATTGTTC+GGG | + | chr2.4:56029915-56029934 | None:intergenic | 30.0% | |
| TCAAGTATACCAAATCAATG+TGG | + | chr2.4:56029634-56029653 | None:intergenic | 30.0% | |
| TTCTAATCTGAGTATCATTC+CGG | - | chr2.4:56029852-56029871 | MS.gene059043:intron | 30.0% | |
| ! | GAAGAATAACCACATTGATT+TGG | - | chr2.4:56029622-56029641 | MS.gene059043:intron | 30.0% |
| ! | GACAAAAGTGATTTTCTTGA+TGG | + | chr2.4:56029315-56029334 | None:intergenic | 30.0% |
| !! | AAGAAAATCACTTTTGTCGA+AGG | - | chr2.4:56029317-56029336 | MS.gene059043:CDS | 30.0% |
| !! | AAGAGTATTGATATCGTTGA+AGG | - | chr2.4:56029272-56029291 | MS.gene059043:CDS | 30.0% |
| AAAGTATGTGTTGCACAAAG+TGG | - | chr2.4:56030098-56030117 | MS.gene059043:intron | 35.0% | |
| ATCTAACTGCGAAATTCACA+CGG | - | chr2.4:56029383-56029402 | MS.gene059043:CDS | 35.0% | |
| ATTGATATCGTTGAAGGAGA+CGG | - | chr2.4:56029278-56029297 | MS.gene059043:CDS | 35.0% | |
| TGATGGCATCAATAACCTTT+GGG | + | chr2.4:56029253-56029272 | None:intergenic | 35.0% | |
| TTTAACCAATGATGTGCAGA+TGG | - | chr2.4:56030069-56030088 | MS.gene059043:intron | 35.0% | |
| TTTCACACTAAGCTTTGCAA+TGG | + | chr2.4:56030244-56030263 | None:intergenic | 35.0% | |
| ! | GGAATAGGTCATCCTTTTAA+CGG | - | chr2.4:56029783-56029802 | MS.gene059043:intron | 35.0% |
| ! | TTGATGGCATCAATAACCTT+TGG | + | chr2.4:56029254-56029273 | None:intergenic | 35.0% |
| !! | AGTGATTTTCTTGATGGTTC+CGG | + | chr2.4:56029309-56029328 | None:intergenic | 35.0% |
| AAATTGTCTGCAGGACCAAA+TGG | - | chr2.4:56030213-56030232 | MS.gene059043:CDS | 40.0% | |
| AACTCAAGAATGGCAAAGCT+AGG | - | chr2.4:56030301-56030320 | MS.gene059043:CDS | 40.0% | |
| ACTCAAGAATGGCAAAGCTA+GGG | - | chr2.4:56030302-56030321 | MS.gene059043:CDS | 40.0% | |
| AGTGAAGAGGAACTCAAGAA+TGG | - | chr2.4:56030291-56030310 | MS.gene059043:CDS | 40.0% | |
| GATGGCATCAATAACCTTTG+GGG | + | chr2.4:56029252-56029271 | None:intergenic | 40.0% | |
| GCTTATCACTACAGCATAGT+TGG | - | chr2.4:56030144-56030163 | MS.gene059043:intron | 40.0% | |
| TATCACTACAGCATAGTTGG+TGG | - | chr2.4:56030147-56030166 | MS.gene059043:intron | 40.0% | |
| TGCAGACAATTTAGCCTCAA+AGG | + | chr2.4:56030205-56030224 | None:intergenic | 40.0% | |
| TTCTTGAGTTCCTCTTCACT+TGG | + | chr2.4:56030291-56030310 | None:intergenic | 40.0% | |
| TTTCACCATCTGCACATCAT+TGG | + | chr2.4:56030077-56030096 | None:intergenic | 40.0% | |
| TTTGCAATGGTTCCTCCATT+TGG | + | chr2.4:56030231-56030250 | None:intergenic | 40.0% | |
| ! | AGAGCTTTGTAAAGTGTAGC+AGG | + | chr2.4:56029206-56029225 | None:intergenic | 40.0% |
| ! | TTTGAGGCTAAATTGTCTGC+AGG | - | chr2.4:56030204-56030223 | MS.gene059043:CDS | 40.0% |
| !! | CATCAATAACCTTTGGGGTA+AGG | + | chr2.4:56029247-56029266 | None:intergenic | 40.0% |
| !! | TAGGGGTGATGGTCTTTTTA+AGG | - | chr2.4:56030320-56030339 | MS.gene059043:CDS | 40.0% |
| !!! | ACAGCATAGTTGGTGGTTTT+GGG | - | chr2.4:56030154-56030173 | MS.gene059043:intron | 40.0% |
| !!! | GGTCTTTTTAAGGCTCTTGA+AGG | - | chr2.4:56030330-56030349 | MS.gene059043:CDS | 40.0% |
| !!! | TACAGCATAGTTGGTGGTTT+TGG | - | chr2.4:56030153-56030172 | MS.gene059043:intron | 40.0% |
| AGTGGAGAAGATCTCCTTTG+AGG | - | chr2.4:56030188-56030207 | MS.gene059043:intron | 45.0% | |
| CTCAAGAATGGCAAAGCTAG+GGG | - | chr2.4:56030303-56030322 | MS.gene059043:CDS | 45.0% | |
| TGTAGCAGGAGCTACAATAG+AGG | + | chr2.4:56029192-56029211 | None:intergenic | 45.0% | |
| ! | GGCTCTTGAAGGTTACGTTT+TGG | - | chr2.4:56030341-56030360 | MS.gene059043:CDS | 45.0% |
| AATGGCAAAGCTAGGGGTGA+TGG | - | chr2.4:56030309-56030328 | MS.gene059043:CDS | 50.0% | |
| AGCAGGAGCTACAATAGAGG+TGG | + | chr2.4:56029189-56029208 | None:intergenic | 50.0% | |
| GAGATCTTCTCCACTGTGTC+TGG | + | chr2.4:56030183-56030202 | None:intergenic | 50.0% | |
| GATATCGTTGAAGGAGACGG+TGG | - | chr2.4:56029281-56029300 | MS.gene059043:CDS | 50.0% | |
| TGATGCTGCACCAAGTGAAG+AGG | - | chr2.4:56030278-56030297 | MS.gene059043:CDS | 50.0% | |
| TGCTGACAACCTTACCCCAA+AGG | - | chr2.4:56029235-56029254 | MS.gene059043:CDS | 50.0% | |
| TTGTCTGCAGGACCAAATGG+AGG | - | chr2.4:56030216-56030235 | MS.gene059043:CDS | 50.0% | |
| ! | TTTTGGGCTTCCAGACACAG+TGG | - | chr2.4:56030170-56030189 | MS.gene059043:intron | 50.0% |
| GTTGAAGGAGACGGTGGCGC+CGG | - | chr2.4:56029287-56029306 | MS.gene059043:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 56029158 | 56030383 | 56029158 | ID=MS.gene059043 |
| chr2.4 | mRNA | 56029158 | 56030383 | 56029158 | ID=MS.gene059043.t1;Parent=MS.gene059043 |
| chr2.4 | exon | 56030203 | 56030383 | 56030203 | ID=MS.gene059043.t1.exon1;Parent=MS.gene059043.t1 |
| chr2.4 | CDS | 56030203 | 56030383 | 56030203 | ID=cds.MS.gene059043.t1;Parent=MS.gene059043.t1 |
| chr2.4 | exon | 56029158 | 56029453 | 56029158 | ID=MS.gene059043.t1.exon2;Parent=MS.gene059043.t1 |
| chr2.4 | CDS | 56029158 | 56029453 | 56029158 | ID=cds.MS.gene059043.t1;Parent=MS.gene059043.t1 |
| Gene Sequence |
| Protein sequence |
