Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46401.t1 | XP_013457582.1 | 96.2 | 264 | 10 | 0 | 1 | 264 | 13 | 276 | 3.60E-147 | 530.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46401.t1 | Q8GXV5 | 71.5 | 256 | 73 | 0 | 9 | 264 | 21 | 276 | 5.2e-110 | 398.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46401.t1 | A0A072URB8 | 96.2 | 264 | 10 | 0 | 1 | 264 | 13 | 276 | 2.6e-147 | 530.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050876 | MS.gene46401 | 0.821582 | 3.74E-53 | -1.69E-46 |
| MS.gene057684 | MS.gene46401 | 0.828539 | 8.50E-55 | -1.69E-46 |
| MS.gene059026 | MS.gene46401 | 0.80888 | 2.49E-50 | -1.69E-46 |
| MS.gene059837 | MS.gene46401 | 0.805661 | 1.20E-49 | -1.69E-46 |
| MS.gene060703 | MS.gene46401 | 0.827002 | 1.99E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 75 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATTGAATGTTCAAAATT+TGG | 0.124447 | 8.4:-23687202 | None:intergenic |
| TAGAGTGGAGTCATAAATTT+TGG | 0.203494 | 8.4:-23682344 | None:intergenic |
| GGGCCATAAACATGACTTTC+TGG | 0.237286 | 8.4:-23684861 | None:intergenic |
| TCACCAGAAAGTCATGTTTA+TGG | 0.245916 | 8.4:+23684858 | MS.gene46401:CDS |
| ATAAAGTGTGAGCCCATTTC+AGG | 0.251326 | 8.4:-23685008 | None:intergenic |
| CCAGCATGGTTTGTTGCTAT+TGG | 0.298210 | 8.4:+23688450 | MS.gene46401:CDS |
| AACTTATCACCTTCAGTATA+TGG | 0.299139 | 8.4:-23686008 | None:intergenic |
| ACAAACCATGCTGGTTATAA+TGG | 0.317445 | 8.4:-23688441 | None:intergenic |
| TTCAGGCGGGTGATGTCATT+TGG | 0.330112 | 8.4:+23689772 | MS.gene46401:CDS |
| AAAGCTACTTCCAATGTCTT+TGG | 0.332406 | 8.4:+23688143 | MS.gene46401:CDS |
| AGGAGAAGGCATTTATCGTT+TGG | 0.348546 | 8.4:+23688474 | MS.gene46401:CDS |
| TGTGAATAGGTACCCGGTTC+AGG | 0.358704 | 8.4:+23689755 | MS.gene46401:intron |
| ATGACATCACCCGCCTGAAC+CGG | 0.360131 | 8.4:-23689768 | None:intergenic |
| AGGGGCTCTGGGACATGATT+TGG | 0.367925 | 8.4:-23687537 | None:intergenic |
| TGGAGAGTTTCTTAATGTTA+AGG | 0.373459 | 8.4:+23688323 | MS.gene46401:CDS |
| CCCTTACTTTGGAGATTAAG+AGG | 0.387626 | 8.4:-23682370 | None:intergenic |
| AGGGTTGTTTAGGTATTCAT+TGG | 0.390429 | 8.4:+23682252 | None:intergenic |
| GGAGAAGGCATTTATCGTTT+GGG | 0.390850 | 8.4:+23688475 | MS.gene46401:CDS |
| TTTGCAGAAAATTCAAGATC+AGG | 0.405975 | 8.4:+23685764 | MS.gene46401:intron |
| TTTATGGCCCATTGCCTGAT+TGG | 0.406017 | 8.4:+23684874 | MS.gene46401:CDS |
| GTCGAATTAACTAGGGGCTC+TGG | 0.407908 | 8.4:-23687549 | None:intergenic |
| GAGATTAAGAGGAGGTAGAG+TGG | 0.413997 | 8.4:-23682359 | None:intergenic |
| GTTTATCCGTCGAATTAACT+AGG | 0.436467 | 8.4:-23687557 | None:intergenic |
| TATTCATTGGCGCAAGAAGA+AGG | 0.459022 | 8.4:+23682265 | MS.gene46401:CDS |
| TCGAATTAACTAGGGGCTCT+GGG | 0.484257 | 8.4:-23687548 | None:intergenic |
| CATGGTTTGTTGCTATTGGA+AGG | 0.492316 | 8.4:+23688454 | MS.gene46401:CDS |
| TAGACACTGCGTGTGAATCC+AGG | 0.492364 | 8.4:-23684813 | None:intergenic |
| TAGGAATAATGTACCTCCCC+TGG | 0.496765 | 8.4:-23687597 | None:intergenic |
| TTTGGCAGGATCAATACATC+GGG | 0.498459 | 8.4:+23684969 | MS.gene46401:intron |
| AAAACCTCTCTACATCATGC+AGG | 0.502363 | 8.4:-23685793 | None:intergenic |
| TTGACGTAGCTTACCATTGT+GGG | 0.503289 | 8.4:-23689809 | None:intergenic |
| AGAGTTACCAATCAGGCAAT+GGG | 0.505467 | 8.4:-23684881 | None:intergenic |
| TCAAACCTTTCAGCTTTGCT+AGG | 0.509684 | 8.4:-23685037 | None:intergenic |
| TTTATCGTTTGGGTGATAGT+TGG | 0.509948 | 8.4:+23688485 | MS.gene46401:CDS |
| GGCGGGTGATGTCATTTGGA+TGG | 0.513354 | 8.4:+23689776 | MS.gene46401:CDS |
| ATTGAATGTTCAAAATTTGG+AGG | 0.514704 | 8.4:-23687199 | None:intergenic |
| CAGCCACTCGTTGAAACTCC+AGG | 0.517778 | 8.4:+23687579 | MS.gene46401:CDS |
| TTTATCCGTCGAATTAACTA+GGG | 0.520493 | 8.4:-23687556 | None:intergenic |
| TCAAATACCACAATAATAGC+AGG | 0.532548 | 8.4:-23687235 | None:intergenic |
| GTCAGAGTCACCAGTGCTCA+TGG | 0.534419 | 8.4:+23685979 | MS.gene46401:CDS |
| AATTCTTCGCTGAGCACAGA+AGG | 0.539539 | 8.4:-23682301 | None:intergenic |
| TGACATCACCCGCCTGAACC+GGG | 0.548155 | 8.4:-23689767 | None:intergenic |
| AAGAGTTACCAATCAGGCAA+TGG | 0.552009 | 8.4:-23684882 | None:intergenic |
| AGATTCATCTTTGTGCTCCA+CGG | 0.553805 | 8.4:+23685952 | MS.gene46401:intron |
| ATATTCAAGAGTTACCAATC+AGG | 0.554162 | 8.4:-23684888 | None:intergenic |
| ATTCATTGGCGCAAGAAGAA+GGG | 0.563652 | 8.4:+23682266 | MS.gene46401:CDS |
| ATGGCTCACACCATGAGCAC+TGG | 0.568857 | 8.4:-23685989 | None:intergenic |
| AAGTCATATGCCAAAGACAT+TGG | 0.570777 | 8.4:-23688153 | None:intergenic |
| AATAGGTACCCGGTTCAGGC+GGG | 0.576983 | 8.4:+23689759 | MS.gene46401:intron |
| GCTTACCATTGTGGGACGAA+TGG | 0.580025 | 8.4:-23689801 | None:intergenic |
| AAACCTCTCTACATCATGCA+GGG | 0.584130 | 8.4:-23685792 | None:intergenic |
| CCAATAGCAACAAACCATGC+TGG | 0.587457 | 8.4:-23688450 | None:intergenic |
| TCCCCTGGAGTTTCAACGAG+TGG | 0.592547 | 8.4:-23687582 | None:intergenic |
| TTATTGTGGTATTTGAACGA+AGG | 0.594533 | 8.4:+23687242 | MS.gene46401:CDS |
| GAATAGGTACCCGGTTCAGG+CGG | 0.594571 | 8.4:+23689758 | MS.gene46401:intron |
| TTTGACGTAGCTTACCATTG+TGG | 0.595739 | 8.4:-23689810 | None:intergenic |
| TTAACATTAAGAAACTCTCC+AGG | 0.597029 | 8.4:-23688321 | None:intergenic |
| AGCCACTCGTTGAAACTCCA+GGG | 0.597354 | 8.4:+23687580 | MS.gene46401:CDS |
| CTTTGGCAGGATCAATACAT+CGG | 0.603198 | 8.4:+23684968 | MS.gene46401:intron |
| TTGTTGCTATTGGAAGGAGA+AGG | 0.606426 | 8.4:+23688460 | MS.gene46401:CDS |
| TGACGCTCCTGCTATTATTG+TGG | 0.607609 | 8.4:+23687228 | MS.gene46401:CDS |
| TTCCCCTGCATGATGTAGAG+AGG | 0.611970 | 8.4:+23685789 | MS.gene46401:CDS |
| ATGTACCTAGCAAAGCTGAA+AGG | 0.629911 | 8.4:+23685032 | MS.gene46401:CDS |
| GAGGTCCATTATAACCAGCA+TGG | 0.636159 | 8.4:+23688436 | MS.gene46401:CDS |
| AGAGCCCCTAGTTAATTCGA+CGG | 0.642003 | 8.4:+23687551 | MS.gene46401:CDS |
| TGGCGCCATTCGTCCCACAA+TGG | 0.657597 | 8.4:+23689796 | MS.gene46401:CDS |
| TGGTGTGAGCCATATACTGA+AGG | 0.678734 | 8.4:+23685999 | MS.gene46401:CDS |
| ACTCGTTGAAACTCCAGGGG+AGG | 0.679003 | 8.4:+23687584 | MS.gene46401:CDS |
| AACCTCTCTACATCATGCAG+GGG | 0.681979 | 8.4:-23685791 | None:intergenic |
| TTATCCGTCGAATTAACTAG+GGG | 0.689589 | 8.4:-23687555 | None:intergenic |
| TGGCAGGATCAATACATCGG+GGG | 0.695468 | 8.4:+23684971 | MS.gene46401:intron |
| TTACTTTGGAGATTAAGAGG+AGG | 0.697440 | 8.4:-23682367 | None:intergenic |
| GCCACTCGTTGAAACTCCAG+GGG | 0.709562 | 8.4:+23687581 | MS.gene46401:CDS |
| TGGTGACTCTGACAACACCG+TGG | 0.731971 | 8.4:-23685969 | None:intergenic |
| TTGGCAGGATCAATACATCG+GGG | 0.751264 | 8.4:+23684970 | MS.gene46401:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 23682254 | 23689821 | 23682254 | ID=MS.gene46401 |
| chr8.4 | mRNA | 23682254 | 23689821 | 23682254 | ID=MS.gene46401.t1;Parent=MS.gene46401 |
| chr8.4 | exon | 23682254 | 23682385 | 23682254 | ID=MS.gene46401.t1.exon1;Parent=MS.gene46401.t1 |
| chr8.4 | CDS | 23682254 | 23682385 | 23682254 | ID=cds.MS.gene46401.t1;Parent=MS.gene46401.t1 |
| chr8.4 | exon | 23684808 | 23684895 | 23684808 | ID=MS.gene46401.t1.exon2;Parent=MS.gene46401.t1 |
| chr8.4 | CDS | 23684808 | 23684895 | 23684808 | ID=cds.MS.gene46401.t1;Parent=MS.gene46401.t1 |
| chr8.4 | exon | 23684977 | 23685053 | 23684977 | ID=MS.gene46401.t1.exon3;Parent=MS.gene46401.t1 |
| chr8.4 | CDS | 23684977 | 23685053 | 23684977 | ID=cds.MS.gene46401.t1;Parent=MS.gene46401.t1 |
| chr8.4 | exon | 23685771 | 23685810 | 23685771 | ID=MS.gene46401.t1.exon4;Parent=MS.gene46401.t1 |
| chr8.4 | CDS | 23685771 | 23685810 | 23685771 | ID=cds.MS.gene46401.t1;Parent=MS.gene46401.t1 |
| chr8.4 | exon | 23685954 | 23686020 | 23685954 | ID=MS.gene46401.t1.exon5;Parent=MS.gene46401.t1 |
| chr8.4 | CDS | 23685954 | 23686020 | 23685954 | ID=cds.MS.gene46401.t1;Parent=MS.gene46401.t1 |
| chr8.4 | exon | 23687178 | 23687263 | 23687178 | ID=MS.gene46401.t1.exon6;Parent=MS.gene46401.t1 |
| chr8.4 | CDS | 23687178 | 23687263 | 23687178 | ID=cds.MS.gene46401.t1;Parent=MS.gene46401.t1 |
| chr8.4 | exon | 23687524 | 23687605 | 23687524 | ID=MS.gene46401.t1.exon7;Parent=MS.gene46401.t1 |
| chr8.4 | CDS | 23687524 | 23687605 | 23687524 | ID=cds.MS.gene46401.t1;Parent=MS.gene46401.t1 |
| chr8.4 | exon | 23688129 | 23688185 | 23688129 | ID=MS.gene46401.t1.exon8;Parent=MS.gene46401.t1 |
| chr8.4 | CDS | 23688129 | 23688185 | 23688129 | ID=cds.MS.gene46401.t1;Parent=MS.gene46401.t1 |
| chr8.4 | exon | 23688306 | 23688344 | 23688306 | ID=MS.gene46401.t1.exon9;Parent=MS.gene46401.t1 |
| chr8.4 | CDS | 23688306 | 23688344 | 23688306 | ID=cds.MS.gene46401.t1;Parent=MS.gene46401.t1 |
| chr8.4 | exon | 23688436 | 23688506 | 23688436 | ID=MS.gene46401.t1.exon10;Parent=MS.gene46401.t1 |
| chr8.4 | CDS | 23688436 | 23688506 | 23688436 | ID=cds.MS.gene46401.t1;Parent=MS.gene46401.t1 |
| chr8.4 | exon | 23689764 | 23689821 | 23689764 | ID=MS.gene46401.t1.exon11;Parent=MS.gene46401.t1 |
| chr8.4 | CDS | 23689764 | 23689821 | 23689764 | ID=cds.MS.gene46401.t1;Parent=MS.gene46401.t1 |
| Gene Sequence |
| Protein sequence |
