Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46426.t1 | KEH34366.1 | 71.1 | 228 | 53 | 3 | 1 | 227 | 4 | 219 | 9.30E-75 | 290 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46426.t1 | A0A072UY95 | 71.1 | 228 | 53 | 3 | 1 | 227 | 4 | 219 | 6.7e-75 | 290.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051932 | MS.gene46426 | 0.871189 | 8.26E-67 | -1.69E-46 |
| MS.gene053491 | MS.gene46426 | 0.841872 | 3.65E-58 | -1.69E-46 |
| MS.gene06044 | MS.gene46426 | 0.849639 | 2.84E-60 | -1.69E-46 |
| MS.gene061245 | MS.gene46426 | 0.836381 | 9.68E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46426.t1 | MTR_3g465350 | 77.193 | 228 | 39 | 3 | 1 | 227 | 4 | 219 | 6.09e-120 | 340 |
| MS.gene46426.t1 | MTR_3g465380 | 38.953 | 172 | 91 | 5 | 24 | 187 | 38 | 203 | 2.07e-22 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 55 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAATAGATCGTCAATATT+AGG | 0.196396 | 1.2:-44192644 | None:intergenic |
| AAATCCAAGTGAAGAAATTA+AGG | 0.250461 | 1.2:+44192174 | MS.gene46426:CDS |
| TGAGTCCGAGTCTCGATTAA+AGG | 0.273789 | 1.2:-44191680 | None:intergenic |
| TCCTCTTAGGACCCTTGTTT+TGG | 0.280514 | 1.2:-44191178 | None:intergenic |
| GAACTAGCACAGCTGATTAA+TGG | 0.293544 | 1.2:+44192224 | MS.gene46426:CDS |
| TATTTCAGTCTGATCCTCTT+AGG | 0.304174 | 1.2:-44191191 | None:intergenic |
| CTCCAACATGTGGTTCAGTT+TGG | 0.312413 | 1.2:+44191096 | MS.gene46426:CDS |
| CCAACATCAAACGGTGCTTT+TGG | 0.325999 | 1.2:-44191934 | None:intergenic |
| GTTTGCAGTAGAGTGGTTAA+AGG | 0.332534 | 1.2:-44191126 | None:intergenic |
| TCCAACATGTGGTTCAGTTT+GGG | 0.349563 | 1.2:+44191097 | MS.gene46426:CDS |
| TTGTTCTTACCTTGATCATA+TGG | 0.360817 | 1.2:-44192305 | None:intergenic |
| AGAACACAACAACATGGAAT+TGG | 0.366539 | 1.2:+44192598 | MS.gene46426:CDS |
| ACATAGGTGGCGTCCACATT+TGG | 0.369942 | 1.2:-44192559 | None:intergenic |
| ATAATTTGAGTCTAAATCAT+TGG | 0.376358 | 1.2:-44192273 | None:intergenic |
| GAAATAGATCGTCAATATTA+GGG | 0.384297 | 1.2:-44192643 | None:intergenic |
| GATACAAAGGTGGTTCTGTT+TGG | 0.388046 | 1.2:-44191073 | None:intergenic |
| AACTAGCACAGCTGATTAAT+GGG | 0.393662 | 1.2:+44192225 | MS.gene46426:CDS |
| GAACACAACAACATGGAATT+GGG | 0.459020 | 1.2:+44192599 | MS.gene46426:CDS |
| TGCAAACTTAGGAGCCAACT+TGG | 0.462105 | 1.2:+44191142 | MS.gene46426:CDS |
| GTTCCCTTAATTTCTTCACT+TGG | 0.466132 | 1.2:-44192178 | None:intergenic |
| GGAATTGGGCATGGAATGGA+TGG | 0.466811 | 1.2:+44192613 | MS.gene46426:CDS |
| GTTTCAAAACCATATGATCA+AGG | 0.472120 | 1.2:+44192296 | MS.gene46426:CDS |
| GTTTCGTGCTCATCCGACAT+AGG | 0.491148 | 1.2:-44192575 | None:intergenic |
| CCAAAAGCACCGTTTGATGT+TGG | 0.496047 | 1.2:+44191934 | MS.gene46426:CDS |
| AATCCAAGTGAAGAAATTAA+GGG | 0.496720 | 1.2:+44192175 | MS.gene46426:CDS |
| GTTGGAGCGTGTGACGTTGA+TGG | 0.506274 | 1.2:+44191952 | MS.gene46426:CDS |
| GTCACTATTAAGACCCAAGT+TGG | 0.507718 | 1.2:-44191156 | None:intergenic |
| AACCACTCTACTGCAAACTT+AGG | 0.532758 | 1.2:+44191131 | MS.gene46426:CDS |
| CTCCTAAGTTTGCAGTAGAG+TGG | 0.536634 | 1.2:-44191133 | None:intergenic |
| AATGTGGACGCCACCTATGT+CGG | 0.552697 | 1.2:+44192562 | MS.gene46426:CDS |
| AAAGGTGGTTCTGTTTGGAA+AGG | 0.562830 | 1.2:-44191068 | None:intergenic |
| TCACACGCTCCAACATCAAA+CGG | 0.566826 | 1.2:-44191943 | None:intergenic |
| ACATGGAATTGGGCATGGAA+TGG | 0.568585 | 1.2:+44192609 | MS.gene46426:CDS |
| TCTTAATAGTGACCAAAACA+AGG | 0.569518 | 1.2:+44191166 | MS.gene46426:CDS |
| GAGTCTAAATCATTGGACAT+TGG | 0.575430 | 1.2:-44192266 | None:intergenic |
| GATCAAGACGGAAGGAAGAT+CGG | 0.579150 | 1.2:+44191710 | MS.gene46426:CDS |
| GACTATTACAAAGCCAAATG+TGG | 0.588677 | 1.2:+44192546 | MS.gene46426:CDS |
| CAACAACATGGAATTGGGCA+TGG | 0.590352 | 1.2:+44192604 | MS.gene46426:CDS |
| ACCAAAACAAGGGTCCTAAG+AGG | 0.590710 | 1.2:+44191177 | MS.gene46426:CDS |
| GTTCTGTTTGGAAAGGAACA+TGG | 0.591842 | 1.2:-44191061 | None:intergenic |
| TCCCAAACTGAACCACATGT+TGG | 0.599459 | 1.2:-44191098 | None:intergenic |
| CAAACTGAACCACATGTTGG+AGG | 0.601431 | 1.2:-44191095 | None:intergenic |
| CACTTAGAGAACATAATGGA+GGG | 0.621926 | 1.2:+44191029 | None:intergenic |
| CCACATGTTGGAGGATACAA+AGG | 0.621996 | 1.2:-44191086 | None:intergenic |
| AGCTCATAGATCAAGACGGA+AGG | 0.626708 | 1.2:+44191702 | MS.gene46426:CDS |
| AAAGAAGCACATCTTAAAGA+CGG | 0.628544 | 1.2:+44191995 | MS.gene46426:CDS |
| CGAAACAGAACACAACAACA+TGG | 0.635296 | 1.2:+44192592 | MS.gene46426:CDS |
| CTACGTCGATCAACAAGCTG+AGG | 0.639200 | 1.2:+44192202 | MS.gene46426:CDS |
| TCGTGCTCATCCGACATAGG+TGG | 0.642330 | 1.2:-44192572 | None:intergenic |
| CCTTTGTATCCTCCAACATG+TGG | 0.647083 | 1.2:+44191086 | MS.gene46426:CDS |
| TGAAACCTTTAATCGAGACT+CGG | 0.649080 | 1.2:+44191675 | MS.gene46426:CDS |
| GCAAACTTAGGAGCCAACTT+GGG | 0.651364 | 1.2:+44191143 | MS.gene46426:CDS |
| ACTCAGCTCATAGATCAAGA+CGG | 0.691685 | 1.2:+44191698 | MS.gene46426:CDS |
| CTTAATAGTGACCAAAACAA+GGG | 0.698882 | 1.2:+44191167 | MS.gene46426:CDS |
| CATGTTGGAGGATACAAAGG+TGG | 0.702436 | 1.2:-44191083 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTCAAAAAAAAAAAAAATTT+GGG | + | chr1.2:44191306-44191325 | MS.gene46426:intron | 10.0% |
| !! | TCAAAAAAAAAAAAAATTTG+GGG | + | chr1.2:44191307-44191326 | MS.gene46426:intron | 10.0% |
| !! | TCTCAAAAAAAAAAAAAATT+TGG | + | chr1.2:44191305-44191324 | MS.gene46426:intron | 10.0% |
| !!! | TAATTATTATTTATTGGTTT+TGG | + | chr1.2:44192415-44192434 | MS.gene46426:intron | 10.0% |
| !! | GTATGCTAATTATTATTTAT+TGG | + | chr1.2:44192409-44192428 | MS.gene46426:intron | 15.0% |
| !! | TAAAAAAGTTAAAGAAATTG+AGG | + | chr1.2:44191750-44191769 | MS.gene46426:CDS | 15.0% |
| !!! | TTACTTTTTTAAAATGACAT+AGG | + | chr1.2:44192524-44192543 | MS.gene46426:intron | 15.0% |
| !!! | TTTAACTTTTTTACTCAAAT+AGG | - | chr1.2:44191743-44191762 | None:intergenic | 15.0% |
| !!! | TTTGACATTTTAAATAATTG+TGG | + | chr1.2:44191274-44191293 | MS.gene46426:intron | 15.0% |
| !! | AAAAGAATTAATTAGTCACT+AGG | - | chr1.2:44192449-44192468 | None:intergenic | 20.0% |
| !! | ACACACAGATATATATATTA+AGG | + | chr1.2:44191336-44191355 | MS.gene46426:intron | 20.0% |
| !! | ATAATTTGAGTCTAAATCAT+TGG | - | chr1.2:44192276-44192295 | None:intergenic | 20.0% |
| !! | TCATCATAATCTAAATTTCA+AGG | - | chr1.2:44191575-44191594 | None:intergenic | 20.0% |
| !! | TTAAAATGTCAAATATCACA+AGG | - | chr1.2:44191267-44191286 | None:intergenic | 20.0% |
| !!! | CAACATATTAGTTTTTGAAA+AGG | - | chr1.2:44192375-44192394 | None:intergenic | 20.0% |
| ! | AAATCCAAGTGAAGAAATTA+AGG | + | chr1.2:44192174-44192193 | MS.gene46426:CDS | 25.0% |
| ! | AATCCAAGTGAAGAAATTAA+GGG | + | chr1.2:44192175-44192194 | MS.gene46426:CDS | 25.0% |
| ! | ACTTTCAGCTGATAATTAAT+GGG | - | chr1.2:44192144-44192163 | None:intergenic | 25.0% |
| ! | AGAAATAGATCGTCAATATT+AGG | - | chr1.2:44192647-44192666 | None:intergenic | 25.0% |
| ! | AGACTGAAATACATGTAATT+TGG | + | chr1.2:44191203-44191222 | MS.gene46426:intron | 25.0% |
| ! | CATAGCTCAATATATTGATA+AGG | + | chr1.2:44192078-44192097 | MS.gene46426:intron | 25.0% |
| ! | GAAATAGATCGTCAATATTA+GGG | - | chr1.2:44192646-44192665 | None:intergenic | 25.0% |
| CACTTTCAGCTGATAATTAA+TGG | - | chr1.2:44192145-44192164 | None:intergenic | 30.0% | |
| CTTAATAGTGACCAAAACAA+GGG | + | chr1.2:44191167-44191186 | MS.gene46426:CDS | 30.0% | |
| GTTTCAAAACCATATGATCA+AGG | + | chr1.2:44192296-44192315 | MS.gene46426:CDS | 30.0% | |
| TCTTAATAGTGACCAAAACA+AGG | + | chr1.2:44191166-44191185 | MS.gene46426:CDS | 30.0% | |
| TTGTTCTTACCTTGATCATA+TGG | - | chr1.2:44192308-44192327 | None:intergenic | 30.0% | |
| ! | AAAGAAGCACATCTTAAAGA+CGG | + | chr1.2:44191995-44192014 | MS.gene46426:CDS | 30.0% |
| AACTAGCACAGCTGATTAAT+GGG | + | chr1.2:44192225-44192244 | MS.gene46426:CDS | 35.0% | |
| AGAACACAACAACATGGAAT+TGG | + | chr1.2:44192598-44192617 | MS.gene46426:CDS | 35.0% | |
| CGCTCATCATAAATATATGC+AGG | + | chr1.2:44191868-44191887 | MS.gene46426:intron | 35.0% | |
| GAACACAACAACATGGAATT+GGG | + | chr1.2:44192599-44192618 | MS.gene46426:CDS | 35.0% | |
| GACTATTACAAAGCCAAATG+TGG | + | chr1.2:44192546-44192565 | MS.gene46426:CDS | 35.0% | |
| GAGTCTAAATCATTGGACAT+TGG | - | chr1.2:44192269-44192288 | None:intergenic | 35.0% | |
| GTTCCCTTAATTTCTTCACT+TGG | - | chr1.2:44192181-44192200 | None:intergenic | 35.0% | |
| TATTTCAGTCTGATCCTCTT+AGG | - | chr1.2:44191194-44191213 | None:intergenic | 35.0% | |
| TGAAACCTTTAATCGAGACT+CGG | + | chr1.2:44191675-44191694 | MS.gene46426:CDS | 35.0% | |
| AACCACTCTACTGCAAACTT+AGG | + | chr1.2:44191131-44191150 | MS.gene46426:CDS | 40.0% | |
| ACACATGCATTATGAGATGC+TGG | - | chr1.2:44191615-44191634 | None:intergenic | 40.0% | |
| ACTCAGCTCATAGATCAAGA+CGG | + | chr1.2:44191698-44191717 | MS.gene46426:CDS | 40.0% | |
| CATGCATTCACCAAAGTCAA+TGG | + | chr1.2:44191480-44191499 | MS.gene46426:intron | 40.0% | |
| CGAAACAGAACACAACAACA+TGG | + | chr1.2:44192592-44192611 | MS.gene46426:CDS | 40.0% | |
| GAACTAGCACAGCTGATTAA+TGG | + | chr1.2:44192224-44192243 | MS.gene46426:CDS | 40.0% | |
| GTCACTATTAAGACCCAAGT+TGG | - | chr1.2:44191159-44191178 | None:intergenic | 40.0% | |
| GTTCTGTTTGGAAAGGAACA+TGG | - | chr1.2:44191064-44191083 | None:intergenic | 40.0% | |
| GTTTGCAGTAGAGTGGTTAA+AGG | - | chr1.2:44191129-44191148 | None:intergenic | 40.0% | |
| TCCAACATGTGGTTCAGTTT+GGG | + | chr1.2:44191097-44191116 | MS.gene46426:CDS | 40.0% | |
| ! | AAAGGTGGTTCTGTTTGGAA+AGG | - | chr1.2:44191071-44191090 | None:intergenic | 40.0% |
| ! | GATACAAAGGTGGTTCTGTT+TGG | - | chr1.2:44191076-44191095 | None:intergenic | 40.0% |
| ACATGGAATTGGGCATGGAA+TGG | + | chr1.2:44192609-44192628 | MS.gene46426:CDS | 45.0% | |
| ACCAAAACAAGGGTCCTAAG+AGG | + | chr1.2:44191177-44191196 | MS.gene46426:CDS | 45.0% | |
| AGCTCATAGATCAAGACGGA+AGG | + | chr1.2:44191702-44191721 | MS.gene46426:CDS | 45.0% | |
| CAAACTGAACCACATGTTGG+AGG | - | chr1.2:44191098-44191117 | None:intergenic | 45.0% | |
| CAACAACATGGAATTGGGCA+TGG | + | chr1.2:44192604-44192623 | MS.gene46426:CDS | 45.0% | |
| CATGTTGGAGGATACAAAGG+TGG | - | chr1.2:44191086-44191105 | None:intergenic | 45.0% | |
| CCACATGTTGGAGGATACAA+AGG | - | chr1.2:44191089-44191108 | None:intergenic | 45.0% | |
| CCTTTGTATCCTCCAACATG+TGG | + | chr1.2:44191086-44191105 | MS.gene46426:CDS | 45.0% | |
| CTCCAACATGTGGTTCAGTT+TGG | + | chr1.2:44191096-44191115 | MS.gene46426:CDS | 45.0% | |
| CTCCTAAGTTTGCAGTAGAG+TGG | - | chr1.2:44191136-44191155 | None:intergenic | 45.0% | |
| GATCAAGACGGAAGGAAGAT+CGG | + | chr1.2:44191710-44191729 | MS.gene46426:CDS | 45.0% | |
| GCAAACTTAGGAGCCAACTT+GGG | + | chr1.2:44191143-44191162 | MS.gene46426:CDS | 45.0% | |
| GGTCGTAGATCGACATCAAA+CGG | + | chr1.2:44191501-44191520 | MS.gene46426:intron | 45.0% | |
| TCACACGCTCCAACATCAAA+CGG | - | chr1.2:44191946-44191965 | None:intergenic | 45.0% | |
| TCCCAAACTGAACCACATGT+TGG | - | chr1.2:44191101-44191120 | None:intergenic | 45.0% | |
| TGAGTCCGAGTCTCGATTAA+AGG | - | chr1.2:44191683-44191702 | None:intergenic | 45.0% | |
| TGCAAACTTAGGAGCCAACT+TGG | + | chr1.2:44191142-44191161 | MS.gene46426:CDS | 45.0% | |
| ! | CCAACATCAAACGGTGCTTT+TGG | - | chr1.2:44191937-44191956 | None:intergenic | 45.0% |
| !! | CCAAAAGCACCGTTTGATGT+TGG | + | chr1.2:44191934-44191953 | MS.gene46426:CDS | 45.0% |
| !! | CGATCTACGACCATTGACTT+TGG | - | chr1.2:44191493-44191512 | None:intergenic | 45.0% |
| !! | TCCTCTTAGGACCCTTGTTT+TGG | - | chr1.2:44191181-44191200 | None:intergenic | 45.0% |
| AATGTGGACGCCACCTATGT+CGG | + | chr1.2:44192562-44192581 | MS.gene46426:CDS | 50.0% | |
| ACATAGGTGGCGTCCACATT+TGG | - | chr1.2:44192562-44192581 | None:intergenic | 50.0% | |
| CTACGTCGATCAACAAGCTG+AGG | + | chr1.2:44192202-44192221 | MS.gene46426:CDS | 50.0% | |
| GGAATTGGGCATGGAATGGA+TGG | + | chr1.2:44192613-44192632 | MS.gene46426:CDS | 50.0% | |
| GTTTCGTGCTCATCCGACAT+AGG | - | chr1.2:44192578-44192597 | None:intergenic | 50.0% | |
| GTTGGAGCGTGTGACGTTGA+TGG | + | chr1.2:44191952-44191971 | MS.gene46426:CDS | 55.0% | |
| TCGTGCTCATCCGACATAGG+TGG | - | chr1.2:44192575-44192594 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 44191044 | 44192670 | 44191044 | ID=MS.gene46426 |
| chr1.2 | mRNA | 44191044 | 44192670 | 44191044 | ID=MS.gene46426.t1;Parent=MS.gene46426 |
| chr1.2 | exon | 44191044 | 44191216 | 44191044 | ID=MS.gene46426.t1.exon1;Parent=MS.gene46426.t1 |
| chr1.2 | CDS | 44191044 | 44191216 | 44191044 | ID=cds.MS.gene46426.t1;Parent=MS.gene46426.t1 |
| chr1.2 | exon | 44191675 | 44191771 | 44191675 | ID=MS.gene46426.t1.exon2;Parent=MS.gene46426.t1 |
| chr1.2 | CDS | 44191675 | 44191771 | 44191675 | ID=cds.MS.gene46426.t1;Parent=MS.gene46426.t1 |
| chr1.2 | exon | 44191890 | 44192016 | 44191890 | ID=MS.gene46426.t1.exon3;Parent=MS.gene46426.t1 |
| chr1.2 | CDS | 44191890 | 44192016 | 44191890 | ID=cds.MS.gene46426.t1;Parent=MS.gene46426.t1 |
| chr1.2 | exon | 44192156 | 44192317 | 44192156 | ID=MS.gene46426.t1.exon4;Parent=MS.gene46426.t1 |
| chr1.2 | CDS | 44192156 | 44192317 | 44192156 | ID=cds.MS.gene46426.t1;Parent=MS.gene46426.t1 |
| chr1.2 | exon | 44192546 | 44192670 | 44192546 | ID=MS.gene46426.t1.exon5;Parent=MS.gene46426.t1 |
| chr1.2 | CDS | 44192546 | 44192670 | 44192546 | ID=cds.MS.gene46426.t1;Parent=MS.gene46426.t1 |
| Gene Sequence |
| Protein sequence |
