Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46629.t1 | XP_003599380.1 | 99.4 | 340 | 2 | 0 | 1 | 340 | 1 | 340 | 5.80E-198 | 699.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46629.t1 | Q9LM02 | 86.3 | 335 | 46 | 0 | 1 | 335 | 1 | 335 | 1.8e-176 | 619.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46629.t1 | B7FHU0 | 99.4 | 340 | 2 | 0 | 1 | 340 | 1 | 340 | 4.2e-198 | 699.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene69360 | MS.gene46629 | PPI |
| MS.gene064797 | MS.gene46629 | PPI |
| MS.gene52804 | MS.gene46629 | PPI |
| MS.gene014381 | MS.gene46629 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46629.t1 | MTR_3g032530 | 99.412 | 340 | 2 | 0 | 1 | 340 | 1 | 340 | 0.0 | 708 |
| MS.gene46629.t1 | MTR_3g032530 | 99.663 | 297 | 1 | 0 | 44 | 340 | 1 | 297 | 0.0 | 623 |
| MS.gene46629.t1 | MTR_3g114780 | 41.667 | 336 | 178 | 4 | 8 | 340 | 39 | 359 | 3.95e-88 | 269 |
| MS.gene46629.t1 | MTR_7g012060 | 53.933 | 89 | 12 | 2 | 148 | 207 | 65 | 153 | 4.96e-23 | 94.0 |
| MS.gene46629.t1 | MTR_1g014690 | 26.882 | 186 | 125 | 3 | 45 | 220 | 69 | 253 | 1.31e-13 | 71.2 |
| MS.gene46629.t1 | MTR_6g069600 | 30.178 | 169 | 105 | 4 | 33 | 201 | 238 | 393 | 1.32e-13 | 72.0 |
| MS.gene46629.t1 | MTR_1g014690 | 30.400 | 125 | 86 | 1 | 96 | 220 | 75 | 198 | 4.19e-12 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46629.t1 | AT5G13710 | 86.269 | 335 | 46 | 0 | 1 | 335 | 1 | 335 | 0.0 | 625 |
| MS.gene46629.t1 | AT5G13710 | 86.269 | 335 | 46 | 0 | 1 | 335 | 1 | 335 | 0.0 | 625 |
| MS.gene46629.t1 | AT1G20330 | 42.515 | 334 | 172 | 5 | 8 | 337 | 39 | 356 | 4.08e-90 | 274 |
| MS.gene46629.t1 | AT1G76090 | 41.176 | 340 | 181 | 5 | 4 | 339 | 34 | 358 | 3.28e-87 | 266 |
| MS.gene46629.t1 | AT1G48600 | 31.176 | 170 | 105 | 5 | 83 | 251 | 256 | 414 | 8.03e-14 | 72.4 |
| MS.gene46629.t1 | AT1G48600 | 31.176 | 170 | 105 | 5 | 83 | 251 | 272 | 430 | 8.20e-14 | 72.4 |
| MS.gene46629.t1 | AT1G73600 | 35.088 | 114 | 72 | 1 | 90 | 203 | 277 | 388 | 1.38e-13 | 72.0 |
| MS.gene46629.t1 | AT1G73600 | 35.088 | 114 | 72 | 1 | 90 | 203 | 291 | 402 | 1.41e-13 | 72.0 |
| MS.gene46629.t1 | AT3G18000 | 31.288 | 163 | 103 | 4 | 89 | 251 | 277 | 430 | 1.51e-13 | 71.6 |
| MS.gene46629.t1 | AT1G64970 | 34.615 | 104 | 68 | 0 | 98 | 201 | 130 | 233 | 5.15e-13 | 69.7 |
Find 80 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGATGTTGGTTGTGGTATT+GGG | 0.139062 | 3.3:-23888223 | MS.gene46629:CDS |
| AATCGAGAAATTTCTCTTAA+TGG | 0.184007 | 3.3:+23888197 | None:intergenic |
| TTGGATGTTGGTTGTGGTAT+TGG | 0.186951 | 3.3:-23888224 | MS.gene46629:CDS |
| CAGAAGCAATACCATATTTC+TGG | 0.200578 | 3.3:+23885075 | None:intergenic |
| TAAGAGAAATTTCTCGATTC+AGG | 0.202299 | 3.3:-23888193 | MS.gene46629:intron |
| ATGGACACCTTGTTGCAAAA+TGG | 0.313547 | 3.3:+23888518 | None:intergenic |
| TCTTCCTTGGTACTTGCCTT+TGG | 0.316688 | 3.3:-23885155 | MS.gene46629:CDS |
| CAGGCACTCAATCGCAAAAC+TGG | 0.331030 | 3.3:-23887343 | MS.gene46629:CDS |
| CACTTCCTTGCTTTACAACT+TGG | 0.357247 | 3.3:-23888376 | MS.gene46629:CDS |
| GAAAGCTAATTATAGTGATA+TGG | 0.367025 | 3.3:-23889258 | MS.gene46629:intron |
| GGATGTTGGTTGTGGTATTG+GGG | 0.388964 | 3.3:-23888222 | MS.gene46629:CDS |
| TGCAGCTCGACATCTGTTAC+CGG | 0.394305 | 3.3:-23887729 | MS.gene46629:intron |
| ATACCTTTCCCCTGGTGATC+TGG | 0.394747 | 3.3:+23887688 | None:intergenic |
| GTGAAGGCTCTGGAGTTTGT+TGG | 0.407221 | 3.3:-23884750 | MS.gene46629:CDS |
| TGAACCCTTTGACTCCCCTT+GGG | 0.410674 | 3.3:+23884720 | None:intergenic |
| TAGAGCTTCAAGACACTTTG+TGG | 0.411789 | 3.3:+23885654 | None:intergenic |
| ATTCAGTTGCTTTCTGAAGC+AGG | 0.422845 | 3.3:+23883794 | None:intergenic |
| TCTTCCGCTGCAGATGGGTA+GGG | 0.426640 | 3.3:-23888432 | MS.gene46629:intron |
| AATATTTCACATACAGCATC+TGG | 0.437219 | 3.3:+23886163 | None:intergenic |
| TTGAACCCTTTGACTCCCCT+TGG | 0.438466 | 3.3:+23884719 | None:intergenic |
| ATGGATTTGGCTTCGAATCT+CGG | 0.442435 | 3.3:-23891673 | MS.gene46629:CDS |
| AAAGATGAAAACCTTCTGTC+CGG | 0.459180 | 3.3:+23888345 | None:intergenic |
| CATCGGACTCTCCTCTTCCT+TGG | 0.478580 | 3.3:-23885168 | MS.gene46629:CDS |
| ATACCATATTTCTGGTGAAA+AGG | 0.482576 | 3.3:+23885083 | None:intergenic |
| GTTTGTTGGACTTGCTCCCA+AGG | 0.488709 | 3.3:-23884736 | MS.gene46629:CDS |
| CTTCCGCTGCAGATGGGTAG+GGG | 0.489356 | 3.3:-23888431 | MS.gene46629:intron |
| CTGCAGGCAGAAATTGAGAT+TGG | 0.490804 | 3.3:-23885703 | MS.gene46629:intron |
| GTACTCGTTATTATTCAATC+CGG | 0.500109 | 3.3:+23887710 | None:intergenic |
| GCTGCAGAGGGTCTAGTTGA+AGG | 0.505379 | 3.3:-23884681 | MS.gene46629:CDS |
| ACATTTGTAGGTGAAGGCTC+TGG | 0.507945 | 3.3:-23884760 | MS.gene46629:intron |
| GAGAAGTATCATGCATCCTA+TGG | 0.508363 | 3.3:-23889299 | MS.gene46629:CDS |
| TTGCTCCCAAGGGGAGTCAA+AGG | 0.513667 | 3.3:-23884725 | MS.gene46629:CDS |
| TTTGTATGTGAAGGTCATAT+GGG | 0.514447 | 3.3:-23885203 | MS.gene46629:intron |
| TTCTTCCGCTGCAGATGGGT+AGG | 0.514609 | 3.3:-23888433 | MS.gene46629:intron |
| AGCTTCCGTCTAACGGCTGT+TGG | 0.514924 | 3.3:-23885109 | MS.gene46629:CDS |
| AAAGGCAAGTACCAAGGAAG+AGG | 0.518855 | 3.3:+23885157 | None:intergenic |
| TACAGCATCTGGTGCATGGC+AGG | 0.521675 | 3.3:+23886174 | None:intergenic |
| ATTTATAGAGTGTTAAAGCC+TGG | 0.522060 | 3.3:-23885881 | MS.gene46629:CDS |
| TAAGCAGCAAAACATTGGCC+AGG | 0.524306 | 3.3:+23885863 | None:intergenic |
| GCAGAAATTGAGATTGGTGA+TGG | 0.534551 | 3.3:-23885697 | MS.gene46629:CDS |
| GGTTCAAGATTTCCTTGAGA+AGG | 0.535966 | 3.3:-23884703 | MS.gene46629:CDS |
| GACCCTCTGCAGCCTTCTCA+AGG | 0.541641 | 3.3:+23884691 | None:intergenic |
| TTTGTGGTCAATCTAATGTC+AGG | 0.541951 | 3.3:+23885670 | None:intergenic |
| ACTTAGCAGCTTCCGTCTAA+CGG | 0.542702 | 3.3:-23885116 | MS.gene46629:CDS |
| TTCATCCTCTTGACCTCCAT+AGG | 0.544369 | 3.3:+23889283 | None:intergenic |
| GGCGTCCAACAGCCGTTAGA+CGG | 0.545051 | 3.3:+23885104 | None:intergenic |
| CTTGAAGCTCTAAAGCAAGC+AGG | 0.547652 | 3.3:-23885643 | MS.gene46629:CDS |
| TTTGTTGGACTTGCTCCCAA+GGG | 0.550647 | 3.3:-23884735 | MS.gene46629:CDS |
| TGGTCTTAAGCCCGGACAGA+AGG | 0.551005 | 3.3:-23888356 | MS.gene46629:intron |
| TTACAACTTGGTCTTAAGCC+CGG | 0.554828 | 3.3:-23888364 | MS.gene46629:CDS |
| TTCCTTGAGAAGGCTGCAGA+GGG | 0.556231 | 3.3:-23884693 | MS.gene46629:CDS |
| AGATTCGAAGCCAAATCCAT+TGG | 0.556251 | 3.3:+23891676 | None:intergenic |
| AAGATGAAAACCTTCTGTCC+GGG | 0.556269 | 3.3:+23888346 | None:intergenic |
| ATTCATAAGCAGCAAAACAT+TGG | 0.559750 | 3.3:+23885858 | None:intergenic |
| TGCTCCCAAGGGGAGTCAAA+GGG | 0.560093 | 3.3:-23884724 | MS.gene46629:CDS |
| ATGGAGGTCAAGAGGATGAA+AGG | 0.562038 | 3.3:-23889280 | MS.gene46629:CDS |
| CACATACAGCATCTGGTGCA+TGG | 0.575106 | 3.3:+23886170 | None:intergenic |
| TCAAAACTATTGTCTTCGAA+TGG | 0.577172 | 3.3:+23886220 | None:intergenic |
| CAGAAATTGAGATTGGTGAT+GGG | 0.583752 | 3.3:-23885696 | MS.gene46629:CDS |
| TTTCCTTGAGAAGGCTGCAG+AGG | 0.588645 | 3.3:-23884694 | MS.gene46629:CDS |
| ATAACGAGTACCAGATCACC+AGG | 0.590364 | 3.3:-23887698 | MS.gene46629:CDS |
| GATGTTGGTTGTGGTATTGG+GGG | 0.596492 | 3.3:-23888221 | MS.gene46629:CDS |
| TAAGACCAAGTTGTAAAGCA+AGG | 0.600321 | 3.3:+23888371 | None:intergenic |
| GTACCAGATCACCAGGGGAA+AGG | 0.607731 | 3.3:-23887691 | MS.gene46629:intron |
| TGCATCCTATGGAGGTCAAG+AGG | 0.610680 | 3.3:-23889288 | MS.gene46629:CDS |
| CAAAACACATACCTTTCCCC+TGG | 0.613010 | 3.3:+23887680 | None:intergenic |
| AGCATCTGGTGCATGGCAGG+TGG | 0.619604 | 3.3:+23886177 | None:intergenic |
| TTGTTGGACTTGCTCCCAAG+GGG | 0.628720 | 3.3:-23884734 | MS.gene46629:CDS |
| GAGAAGTGACTCTTGTCCAA+AGG | 0.629432 | 3.3:+23885139 | None:intergenic |
| GCAGAGGGTCTAGTTGAAGG+TGG | 0.639355 | 3.3:-23884678 | MS.gene46629:CDS |
| ATGGGAGAAAGATCTAGCAT+CGG | 0.642273 | 3.3:-23885185 | MS.gene46629:CDS |
| AAGTATCATGCATCCTATGG+AGG | 0.651186 | 3.3:-23889296 | MS.gene46629:CDS |
| GAGAGAGACGCACTTATCGA+CGG | 0.652088 | 3.3:+23891618 | None:intergenic |
| CAAGACTTGCAACTTCGTCA+AGG | 0.656952 | 3.3:-23887314 | MS.gene46629:intron |
| TTGTCCAAAGGCAAGTACCA+AGG | 0.664739 | 3.3:+23885151 | None:intergenic |
| ACTCAATCGCAAAACTGGAG+TGG | 0.680106 | 3.3:-23887338 | MS.gene46629:CDS |
| GATTTGGCTTCGAATCTCGG+TGG | 0.689368 | 3.3:-23891670 | MS.gene46629:CDS |
| AACGAGTACCAGATCACCAG+GGG | 0.706914 | 3.3:-23887696 | MS.gene46629:CDS |
| TAACGAGTACCAGATCACCA+GGG | 0.744545 | 3.3:-23887697 | MS.gene46629:CDS |
| AGAGACGCACTTATCGACGG+CGG | 0.756178 | 3.3:+23891621 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 23883796 | 23891695 | 23883796 | ID=MS.gene46629 |
| chr3.3 | mRNA | 23883796 | 23891695 | 23883796 | ID=MS.gene46629.t1;Parent=MS.gene46629 |
| chr3.3 | exon | 23891631 | 23891695 | 23891631 | ID=MS.gene46629.t1.exon1;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23891631 | 23891695 | 23891631 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23889259 | 23889325 | 23889259 | ID=MS.gene46629.t1.exon2;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23889259 | 23889325 | 23889259 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23888526 | 23888599 | 23888526 | ID=MS.gene46629.t1.exon3;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23888526 | 23888599 | 23888526 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23888357 | 23888441 | 23888357 | ID=MS.gene46629.t1.exon4;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23888357 | 23888441 | 23888357 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23888194 | 23888249 | 23888194 | ID=MS.gene46629.t1.exon5;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23888194 | 23888249 | 23888194 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23887692 | 23887746 | 23887692 | ID=MS.gene46629.t1.exon6;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23887692 | 23887746 | 23887692 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23887315 | 23887362 | 23887315 | ID=MS.gene46629.t1.exon7;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23887315 | 23887362 | 23887315 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23886177 | 23886260 | 23886177 | ID=MS.gene46629.t1.exon8;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23886177 | 23886260 | 23886177 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23885805 | 23885921 | 23885805 | ID=MS.gene46629.t1.exon9;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23885805 | 23885921 | 23885805 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23885636 | 23885719 | 23885636 | ID=MS.gene46629.t1.exon10;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23885636 | 23885719 | 23885636 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23885087 | 23885212 | 23885087 | ID=MS.gene46629.t1.exon11;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23885087 | 23885212 | 23885087 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23884675 | 23884772 | 23884675 | ID=MS.gene46629.t1.exon12;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23884675 | 23884772 | 23884675 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| chr3.3 | exon | 23883796 | 23883859 | 23883796 | ID=MS.gene46629.t1.exon13;Parent=MS.gene46629.t1 |
| chr3.3 | CDS | 23883796 | 23883859 | 23883796 | ID=cds.MS.gene46629.t1;Parent=MS.gene46629.t1 |
| Gene Sequence |
| Protein sequence |
