Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48839.t1 | RHN59397.1 | 95 | 119 | 6 | 0 | 1 | 119 | 22 | 140 | 2.10E-54 | 221.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48839.t1 | G7JIA9 | 95.0 | 119 | 6 | 0 | 1 | 119 | 1 | 119 | 1.5e-54 | 221.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049850 | MS.gene48839 | 0.80705 | 6.11E-50 | -1.69E-46 |
| MS.gene050020 | MS.gene48839 | 0.8009 | 1.16E-48 | -1.69E-46 |
| MS.gene050807 | MS.gene48839 | 0.800707 | 1.27E-48 | -1.69E-46 |
| MS.gene051247 | MS.gene48839 | 0.808795 | 2.60E-50 | -1.69E-46 |
| MS.gene05157 | MS.gene48839 | 0.816425 | 5.57E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene48839 | 0.834341 | 3.17E-56 | -1.69E-46 |
| MS.gene051720 | MS.gene48839 | 0.848744 | 5.04E-60 | -1.69E-46 |
| MS.gene053226 | MS.gene48839 | 0.8027 | 4.95E-49 | -1.69E-46 |
| MS.gene054775 | MS.gene48839 | 0.815887 | 7.34E-52 | -1.69E-46 |
| MS.gene054801 | MS.gene48839 | 0.816366 | 5.74E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene48839 | 0.824161 | 9.38E-54 | -1.69E-46 |
| MS.gene055466 | MS.gene48839 | 0.838897 | 2.19E-57 | -1.69E-46 |
| MS.gene055468 | MS.gene48839 | 0.811831 | 5.75E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48839.t1 | MTR_4g028110 | 94.958 | 119 | 6 | 0 | 1 | 119 | 1 | 119 | 1.98e-78 | 226 |
| MS.gene48839.t1 | MTR_7g073270 | 49.593 | 123 | 52 | 5 | 4 | 119 | 1 | 120 | 1.09e-23 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCAGAACCAAGATTTCTA+AGG | 0.315908 | 4.4:-74285285 | MS.gene48839:CDS |
| GACACTGACCTTAGAAATCT+TGG | 0.321072 | 4.4:+74285277 | None:intergenic |
| GCAATGATTCGAAAGAGATT+TGG | 0.327865 | 4.4:-74285391 | MS.gene48839:CDS |
| TCAGAATCCACTCTCTTTCA+AGG | 0.337545 | 4.4:-74285092 | MS.gene48839:CDS |
| GGAAGACGTTGTCGCGGTTC+CGG | 0.343136 | 4.4:-74285193 | MS.gene48839:CDS |
| CTTAAACACCTTTCGAAAAC+CGG | 0.347709 | 4.4:+74285174 | None:intergenic |
| AAATTGTTCAAACCATTTCT+TGG | 0.349128 | 4.4:-74285151 | MS.gene48839:CDS |
| CAAAGGATAGCCTCTCAAAT+TGG | 0.423678 | 4.4:+74285344 | None:intergenic |
| TGAGTGACAAGAGGAACTGC+TGG | 0.485543 | 4.4:+74285229 | None:intergenic |
| AGCAGTTCCTCTTGTCACTC+AGG | 0.488848 | 4.4:-74285227 | MS.gene48839:CDS |
| TTTGTTGAGTTGAGACTCAA+AGG | 0.523465 | 4.4:+74285327 | None:intergenic |
| TGATAAGCTTCTTGATAACA+TGG | 0.525013 | 4.4:+74285303 | None:intergenic |
| CTAAATGCCTTGAAAGAGAG+TGG | 0.545008 | 4.4:+74285085 | None:intergenic |
| GTCACTCAGGAGGCGAAGCA+AGG | 0.560619 | 4.4:-74285214 | MS.gene48839:CDS |
| AGGCATTTAGTAGATCATTG+AGG | 0.589944 | 4.4:-74285072 | MS.gene48839:CDS |
| TTCGCCTCCTGAGTGACAAG+AGG | 0.607306 | 4.4:+74285220 | None:intergenic |
| ATTTGTTAGACCAATTTGAG+AGG | 0.623039 | 4.4:-74285354 | MS.gene48839:CDS |
| AGTTCCTCTTGTCACTCAGG+AGG | 0.660481 | 4.4:-74285224 | MS.gene48839:CDS |
| AAGCAAGGAAGACGTTGTCG+CGG | 0.700615 | 4.4:-74285199 | MS.gene48839:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTGTTCAAACCATTTCT+TGG | - | chr4.4:74285318-74285337 | MS.gene48839:CDS | 25.0% |
| CCATTTCTTGGTAGAAAAAA+TGG | - | chr4.4:74285330-74285349 | MS.gene48839:CDS | 30.0% | |
| TATCAGAACCAAGATTTCTA+AGG | - | chr4.4:74285184-74285203 | MS.gene48839:CDS | 30.0% | |
| ! | ATTTGTTAGACCAATTTGAG+AGG | - | chr4.4:74285115-74285134 | MS.gene48839:CDS | 30.0% |
| ! | TGATAAGCTTCTTGATAACA+TGG | + | chr4.4:74285169-74285188 | None:intergenic | 30.0% |
| !!! | CCATTTTTTCTACCAAGAAA+TGG | + | chr4.4:74285333-74285352 | None:intergenic | 30.0% |
| AGGCATTTAGTAGATCATTG+AGG | - | chr4.4:74285397-74285416 | MS.gene48839:CDS | 35.0% | |
| CTTAAACACCTTTCGAAAAC+CGG | + | chr4.4:74285298-74285317 | None:intergenic | 35.0% | |
| GCAATGATTCGAAAGAGATT+TGG | - | chr4.4:74285078-74285097 | MS.gene48839:CDS | 35.0% | |
| TTGGTAGAAAAAATGGAGCA+AGG | - | chr4.4:74285337-74285356 | MS.gene48839:CDS | 35.0% | |
| !! | TTTGTTGAGTTGAGACTCAA+AGG | + | chr4.4:74285145-74285164 | None:intergenic | 35.0% |
| CAAAGGATAGCCTCTCAAAT+TGG | + | chr4.4:74285128-74285147 | None:intergenic | 40.0% | |
| CTAAATGCCTTGAAAGAGAG+TGG | + | chr4.4:74285387-74285406 | None:intergenic | 40.0% | |
| GACACTGACCTTAGAAATCT+TGG | + | chr4.4:74285195-74285214 | None:intergenic | 40.0% | |
| TCAGAATCCACTCTCTTTCA+AGG | - | chr4.4:74285377-74285396 | MS.gene48839:CDS | 40.0% | |
| AAGCAAGGAAGACGTTGTCG+CGG | - | chr4.4:74285270-74285289 | MS.gene48839:CDS | 50.0% | |
| AGCAGTTCCTCTTGTCACTC+AGG | - | chr4.4:74285242-74285261 | MS.gene48839:CDS | 50.0% | |
| AGTTCCTCTTGTCACTCAGG+AGG | - | chr4.4:74285245-74285264 | MS.gene48839:CDS | 50.0% | |
| TGAGTGACAAGAGGAACTGC+TGG | + | chr4.4:74285243-74285262 | None:intergenic | 50.0% | |
| TTCGCCTCCTGAGTGACAAG+AGG | + | chr4.4:74285252-74285271 | None:intergenic | 55.0% | |
| ! | TCGCGGTTCCGGTTTTCGAA+AGG | - | chr4.4:74285287-74285306 | MS.gene48839:CDS | 55.0% |
| GGAAGACGTTGTCGCGGTTC+CGG | - | chr4.4:74285276-74285295 | MS.gene48839:CDS | 60.0% | |
| GTCACTCAGGAGGCGAAGCA+AGG | - | chr4.4:74285255-74285274 | MS.gene48839:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 74285066 | 74285425 | 74285066 | ID=MS.gene48839 |
| chr4.4 | mRNA | 74285066 | 74285425 | 74285066 | ID=MS.gene48839.t1;Parent=MS.gene48839 |
| chr4.4 | exon | 74285066 | 74285425 | 74285066 | ID=MS.gene48839.t1.exon1;Parent=MS.gene48839.t1 |
| chr4.4 | CDS | 74285066 | 74285425 | 74285066 | ID=cds.MS.gene48839.t1;Parent=MS.gene48839.t1 |
| Gene Sequence |
| Protein sequence |
