Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49569.t1 | XP_003613602.1 | 83.1 | 65 | 11 | 0 | 2 | 66 | 75 | 139 | 2.40E-21 | 111.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49569.t1 | Q9M9S3 | 74.2 | 62 | 16 | 0 | 5 | 66 | 77 | 138 | 3.5e-21 | 102.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49569.t1 | G7KB19 | 83.1 | 65 | 11 | 0 | 2 | 66 | 75 | 139 | 1.7e-21 | 111.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene49569.t1 | TF | Whirly |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049865 | MS.gene49569 | 0.806447 | 8.19E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene49569 | 0.801453 | 8.94E-49 | -1.69E-46 |
| MS.gene050876 | MS.gene49569 | 0.815243 | 1.02E-51 | -1.69E-46 |
| MS.gene05087 | MS.gene49569 | 0.800811 | 1.21E-48 | -1.69E-46 |
| MS.gene050904 | MS.gene49569 | 0.82621 | 3.07E-54 | -1.69E-46 |
| MS.gene053083 | MS.gene49569 | -0.800436 | 1.44E-48 | -1.69E-46 |
| MS.gene054638 | MS.gene49569 | 0.80937 | 1.96E-50 | -1.69E-46 |
| MS.gene055765 | MS.gene49569 | -0.818174 | 2.25E-52 | -1.69E-46 |
| MS.gene056450 | MS.gene49569 | 0.806394 | 8.40E-50 | -1.69E-46 |
| MS.gene057872 | MS.gene49569 | 0.820747 | 5.83E-53 | -1.69E-46 |
| MS.gene059837 | MS.gene49569 | 0.829194 | 5.90E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49569.t1 | MTR_5g038560 | 81.818 | 66 | 12 | 0 | 2 | 67 | 75 | 140 | 2.01e-31 | 110 |
| MS.gene49569.t1 | MTR_8g468570 | 65.714 | 70 | 24 | 0 | 1 | 70 | 73 | 142 | 3.40e-28 | 102 |
| MS.gene49569.t1 | MTR_7g116270 | 45.763 | 59 | 32 | 0 | 8 | 66 | 46 | 104 | 1.28e-14 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49569.t1 | AT1G14410 | 73.016 | 63 | 17 | 0 | 5 | 67 | 77 | 139 | 7.65e-28 | 101 |
| MS.gene49569.t1 | AT2G02740 | 70.000 | 60 | 18 | 0 | 8 | 67 | 84 | 143 | 1.74e-25 | 95.9 |
| MS.gene49569.t1 | AT1G71260 | 45.763 | 59 | 32 | 0 | 8 | 66 | 53 | 111 | 2.31e-14 | 65.9 |
Find 24 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCCAAAGCGAATCAGATT+TGG | 0.304457 | 5.1:+29248083 | MS.gene49569:CDS |
| ACATCCAATGAAGTGAATTC+TGG | 0.345501 | 5.1:-29246932 | None:intergenic |
| ACCTCCAGAATTCACTTCAT+TGG | 0.371286 | 5.1:+29246928 | MS.gene49569:CDS |
| GCTTTCTGCTCCAATCATAT+TGG | 0.384421 | 5.1:-29247148 | None:intergenic |
| GCTTCGAGGTTCTCAATTTG+AGG | 0.418961 | 5.1:+29248105 | MS.gene49569:CDS |
| CTACAGTTTGCTCCTTCTGC+CGG | 0.434257 | 5.1:+29247119 | MS.gene49569:CDS |
| CGACCAACATAAACCCTTAG+TGG | 0.451636 | 5.1:-29246863 | None:intergenic |
| AAGCCAAATCTGATTCGCTT+TGG | 0.452589 | 5.1:-29248086 | None:intergenic |
| CCAATCATATTGGCGAAAAC+CGG | 0.464995 | 5.1:-29247138 | None:intergenic |
| GAAGTGAATTCTGGAGGTAC+AGG | 0.480485 | 5.1:-29246923 | None:intergenic |
| CAGTTAGAGAATTGCCACTA+AGG | 0.486519 | 5.1:+29246849 | MS.gene49569:intron |
| TTGGTCGTTCAGTTTACAAA+GGG | 0.502765 | 5.1:+29246879 | MS.gene49569:CDS |
| GCGAATCAGATTTGGCTTCG+AGG | 0.507872 | 5.1:+29248091 | MS.gene49569:CDS |
| AGTTAGAGAATTGCCACTAA+GGG | 0.538677 | 5.1:+29246850 | MS.gene49569:intron |
| ATATGATTGGAGCAGAAAGC+AGG | 0.558461 | 5.1:+29247151 | MS.gene49569:CDS |
| GCTTTGGCAGAACGAGGTGG+AGG | 0.563073 | 5.1:-29248070 | None:intergenic |
| GTTGGTCGTTCAGTTTACAA+AGG | 0.578583 | 5.1:+29246878 | MS.gene49569:CDS |
| GCATTCAAGATATCTAAAGA+AGG | 0.582740 | 5.1:+29247086 | MS.gene49569:CDS |
| CAAAGGGAAAGCTGTGCTCA+CGG | 0.588472 | 5.1:+29246895 | MS.gene49569:CDS |
| TTGCCACTAAGGGTTTATGT+TGG | 0.590045 | 5.1:+29246860 | MS.gene49569:CDS |
| TATTGGCGAAAACCGGCAGA+AGG | 0.609525 | 5.1:-29247131 | None:intergenic |
| TTCGCTTTGGCAGAACGAGG+TGG | 0.617373 | 5.1:-29248073 | None:intergenic |
| TCCAATGAAGTGAATTCTGG+AGG | 0.645641 | 5.1:-29246929 | None:intergenic |
| TGATTCGCTTTGGCAGAACG+AGG | 0.671684 | 5.1:-29248076 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAAAAAATAAAGAAAG+GGG | + | chr5.1:29247834-29247853 | MS.gene49569:intron | 10.0% |
| !!! | TTATTGTTTTTTTATCATCA+TGG | + | chr5.1:29246778-29246797 | MS.gene49569:intron | 15.0% |
| !! | GTTATTGTTCTTCTATAATT+TGG | + | chr5.1:29247177-29247196 | MS.gene49569:intron | 20.0% |
| !! | GTTTGTAAAAAAATTGCTAT+TGG | + | chr5.1:29247670-29247689 | MS.gene49569:intron | 20.0% |
| !!! | TCTAAATTTCTCATGTTTTA+TGG | + | chr5.1:29247001-29247020 | MS.gene49569:intron | 20.0% |
| !!! | TTTTATGGAATTATGAATGT+TGG | + | chr5.1:29247016-29247035 | MS.gene49569:intron | 20.0% |
| ! | CAAAAAATGGTGTAAATGTA+GGG | - | chr5.1:29247606-29247625 | None:intergenic | 25.0% |
| ! | TTGGACTGTTTATTTAGTTA+TGG | + | chr5.1:29247035-29247054 | MS.gene49569:intron | 25.0% |
| !! | GTACTTATTTATTAGTCTCT+TGG | + | chr5.1:29247520-29247539 | MS.gene49569:intron | 25.0% |
| !!! | CAATTTTTTTACAAACTGCA+GGG | - | chr5.1:29247666-29247685 | None:intergenic | 25.0% |
| AAAACACGATACAAAGAGAT+AGG | + | chr5.1:29247870-29247889 | MS.gene49569:intron | 30.0% | |
| AAACACGATACAAAGAGATA+GGG | + | chr5.1:29247871-29247890 | MS.gene49569:intron | 30.0% | |
| AGTACAATGCTGTCAAATAT+TGG | + | chr5.1:29247321-29247340 | MS.gene49569:intron | 30.0% | |
| ATTACACTGAAGTAGAATCA+TGG | - | chr5.1:29248048-29248067 | None:intergenic | 30.0% | |
| CCAAAAAATGGTGTAAATGT+AGG | - | chr5.1:29247607-29247626 | None:intergenic | 30.0% | |
| CTTAGTGGAATTTGAACAAA+TGG | + | chr5.1:29247274-29247293 | MS.gene49569:intron | 30.0% | |
| GCATTCAAGATATCTAAAGA+AGG | + | chr5.1:29247086-29247105 | MS.gene49569:CDS | 30.0% | |
| TCTAGGTTTAAATGTGTCAT+TGG | + | chr5.1:29247579-29247598 | MS.gene49569:intron | 30.0% | |
| TTGTACTAAATCTAGTATCG+CGG | - | chr5.1:29247308-29247327 | None:intergenic | 30.0% | |
| ! | TGTCAAATATTGGCTACTAT+AGG | + | chr5.1:29247331-29247350 | MS.gene49569:intron | 30.0% |
| !! | GTTCTTCTATAATTTGGTGT+CGG | + | chr5.1:29247183-29247202 | MS.gene49569:intron | 30.0% |
| !! | TTTGTGTGTTTTTGTAGTCA+GGG | + | chr5.1:29247063-29247082 | MS.gene49569:intron | 30.0% |
| !! | TTTTGTGTGTTTTTGTAGTC+AGG | + | chr5.1:29247062-29247081 | MS.gene49569:intron | 30.0% |
| !!! | CCTACATTTACACCATTTTT+TGG | + | chr5.1:29247604-29247623 | MS.gene49569:intron | 30.0% |
| !!! | GCAATTTTTTTACAAACTGC+AGG | - | chr5.1:29247667-29247686 | None:intergenic | 30.0% |
| AAACCCAATGTTGTCAATTG+TGG | - | chr5.1:29247499-29247518 | None:intergenic | 35.0% | |
| AAATAGCAGCTATTGCTTAG+TGG | + | chr5.1:29247259-29247278 | MS.gene49569:intron | 35.0% | |
| AACACGATACAAAGAGATAG+GGG | + | chr5.1:29247872-29247891 | MS.gene49569:intron | 35.0% | |
| ACATCCAATGAAGTGAATTC+TGG | - | chr5.1:29246935-29246954 | None:intergenic | 35.0% | |
| ACCGCTGTTAAAAAAACAGA+CGG | + | chr5.1:29247705-29247724 | MS.gene49569:intron | 35.0% | |
| AGTTAGAGAATTGCCACTAA+GGG | + | chr5.1:29246850-29246869 | MS.gene49569:intron | 35.0% | |
| GATCCACAATTGACAACATT+GGG | + | chr5.1:29247493-29247512 | MS.gene49569:intron | 35.0% | |
| TGATCCACAATTGACAACAT+TGG | + | chr5.1:29247492-29247511 | MS.gene49569:intron | 35.0% | |
| TTGGTCGTTCAGTTTACAAA+GGG | + | chr5.1:29246879-29246898 | MS.gene49569:CDS | 35.0% | |
| TTTAACAGCGGTTTAACAGA+AGG | - | chr5.1:29247697-29247716 | None:intergenic | 35.0% | |
| ! | AGCCAAACAAAAAAAAGTGC+AGG | - | chr5.1:29247638-29247657 | None:intergenic | 35.0% |
| ! | ATAGCTGCTATTTGACAACA+CGG | - | chr5.1:29247252-29247271 | None:intergenic | 35.0% |
| ! | GCCAAACAAAAAAAAGTGCA+GGG | - | chr5.1:29247637-29247656 | None:intergenic | 35.0% |
| !! | TTGTGTGTTTTTGTAGTCAG+GGG | + | chr5.1:29247064-29247083 | MS.gene49569:intron | 35.0% |
| !!! | TCCCTGCACTTTTTTTTGTT+TGG | + | chr5.1:29247633-29247652 | MS.gene49569:intron | 35.0% |
| !!! | TCCGTCTGTTTTTTTAACAG+CGG | - | chr5.1:29247709-29247728 | None:intergenic | 35.0% |
| ACACGATACAAAGAGATAGG+GGG | + | chr5.1:29247873-29247892 | MS.gene49569:intron | 40.0% | |
| ACCTCCAGAATTCACTTCAT+TGG | + | chr5.1:29246928-29246947 | MS.gene49569:CDS | 40.0% | |
| AGTACAACGTTGTCAAGTAG+CGG | + | chr5.1:29247408-29247427 | MS.gene49569:intron | 40.0% | |
| ATATCACACCAGAAACGAGA+AGG | - | chr5.1:29247956-29247975 | None:intergenic | 40.0% | |
| ATATGATTGGAGCAGAAAGC+AGG | + | chr5.1:29247151-29247170 | MS.gene49569:CDS | 40.0% | |
| CAGGGACTGAAACCAAAAAA+TGG | - | chr5.1:29247619-29247638 | None:intergenic | 40.0% | |
| CAGTTAGAGAATTGCCACTA+AGG | + | chr5.1:29246849-29246868 | MS.gene49569:intron | 40.0% | |
| CCAATCATATTGGCGAAAAC+CGG | - | chr5.1:29247141-29247160 | None:intergenic | 40.0% | |
| GCTTTCTGCTCCAATCATAT+TGG | - | chr5.1:29247151-29247170 | None:intergenic | 40.0% | |
| GTTGGTCGTTCAGTTTACAA+AGG | + | chr5.1:29246878-29246897 | MS.gene49569:CDS | 40.0% | |
| TATCACACCAGAAACGAGAA+GGG | - | chr5.1:29247955-29247974 | None:intergenic | 40.0% | |
| TCCAATGAAGTGAATTCTGG+AGG | - | chr5.1:29246932-29246951 | None:intergenic | 40.0% | |
| TTGCCACTAAGGGTTTATGT+TGG | + | chr5.1:29246860-29246879 | MS.gene49569:CDS | 40.0% | |
| ! | AAGCCAAATCTGATTCGCTT+TGG | - | chr5.1:29248089-29248108 | None:intergenic | 40.0% |
| !!! | TTTGTTTTTAGTGTGCCACG+TGG | - | chr5.1:29247758-29247777 | None:intergenic | 40.0% |
| ACGAGAAGGGAATCGCAAAA+TGG | - | chr5.1:29247942-29247961 | None:intergenic | 45.0% | |
| CGACCAACATAAACCCTTAG+TGG | - | chr5.1:29246866-29246885 | None:intergenic | 45.0% | |
| CTGCCAAAGCGAATCAGATT+TGG | + | chr5.1:29248083-29248102 | MS.gene49569:CDS | 45.0% | |
| GATACAAAGAGATAGGGGGT+GGG | + | chr5.1:29247877-29247896 | MS.gene49569:intron | 45.0% | |
| GCTATAGCATTGTAGTGCAG+TGG | + | chr5.1:29247441-29247460 | MS.gene49569:intron | 45.0% | |
| GCTTCGAGGTTCTCAATTTG+AGG | + | chr5.1:29248105-29248124 | MS.gene49569:CDS | 45.0% | |
| ! | AGGGGGTGGGAATGAATTTT+AGG | + | chr5.1:29247890-29247909 | MS.gene49569:intron | 45.0% |
| ! | CCGGTTTTCGCCAATATGAT+TGG | + | chr5.1:29247138-29247157 | MS.gene49569:CDS | 45.0% |
| ! | GAAGTGAATTCTGGAGGTAC+AGG | - | chr5.1:29246926-29246945 | None:intergenic | 45.0% |
| ! | GGGGGTGGGAATGAATTTTA+GGG | + | chr5.1:29247891-29247910 | MS.gene49569:intron | 45.0% |
| !! | AAAAATAAAAAATAAAGAAA+GGG | + | chr5.1:29247833-29247852 | MS.gene49569:intron | 5.0% |
| !! | TAAAAATAAAAAATAAAGAA+AGG | + | chr5.1:29247832-29247851 | MS.gene49569:intron | 5.0% |
| !!! | TTATAATATATATATTTTCT+AGG | + | chr5.1:29247562-29247581 | MS.gene49569:intron | 5.0% |
| CAAAGGGAAAGCTGTGCTCA+CGG | + | chr5.1:29246895-29246914 | MS.gene49569:CDS | 50.0% | |
| CGATACAAAGAGATAGGGGG+TGG | + | chr5.1:29247876-29247895 | MS.gene49569:intron | 50.0% | |
| TATTGGCGAAAACCGGCAGA+AGG | - | chr5.1:29247134-29247153 | None:intergenic | 50.0% | |
| TGATTCGCTTTGGCAGAACG+AGG | - | chr5.1:29248079-29248098 | None:intergenic | 50.0% | |
| TGCGATTCCCTTCTCGTTTC+TGG | + | chr5.1:29247945-29247964 | MS.gene49569:intron | 50.0% | |
| ! | CTACAGTTTGCTCCTTCTGC+CGG | + | chr5.1:29247119-29247138 | MS.gene49569:CDS | 50.0% |
| ! | GCGAATCAGATTTGGCTTCG+AGG | + | chr5.1:29248091-29248110 | MS.gene49569:CDS | 50.0% |
| ACCAGTTATTGCACGCCACG+TGG | + | chr5.1:29247740-29247759 | MS.gene49569:intron | 55.0% | |
| CGTGCAATAACTGGTCCACG+TGG | - | chr5.1:29247735-29247754 | None:intergenic | 55.0% | |
| TTCGCTTTGGCAGAACGAGG+TGG | - | chr5.1:29248076-29248095 | None:intergenic | 55.0% | |
| ! | AAAACAGACGGAGTGCCACG+TGG | + | chr5.1:29247717-29247736 | MS.gene49569:intron | 55.0% |
| GCCACGTGGCGTGCAATAAC+TGG | - | chr5.1:29247744-29247763 | None:intergenic | 60.0% | |
| GCTTTGGCAGAACGAGGTGG+AGG | - | chr5.1:29248073-29248092 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 29246753 | 29248139 | 29246753 | ID=MS.gene49569 |
| chr5.1 | mRNA | 29246753 | 29248139 | 29246753 | ID=MS.gene49569.t1;Parent=MS.gene49569 |
| chr5.1 | exon | 29246753 | 29246756 | 29246753 | ID=MS.gene49569.t1.exon1;Parent=MS.gene49569.t1 |
| chr5.1 | CDS | 29246753 | 29246756 | 29246753 | ID=cds.MS.gene49569.t1;Parent=MS.gene49569.t1 |
| chr5.1 | exon | 29246852 | 29246952 | 29246852 | ID=MS.gene49569.t1.exon2;Parent=MS.gene49569.t1 |
| chr5.1 | CDS | 29246852 | 29246952 | 29246852 | ID=cds.MS.gene49569.t1;Parent=MS.gene49569.t1 |
| chr5.1 | exon | 29247080 | 29247172 | 29247080 | ID=MS.gene49569.t1.exon3;Parent=MS.gene49569.t1 |
| chr5.1 | CDS | 29247080 | 29247172 | 29247080 | ID=cds.MS.gene49569.t1;Parent=MS.gene49569.t1 |
| chr5.1 | exon | 29248062 | 29248139 | 29248062 | ID=MS.gene49569.t1.exon4;Parent=MS.gene49569.t1 |
| chr5.1 | CDS | 29248062 | 29248139 | 29248062 | ID=cds.MS.gene49569.t1;Parent=MS.gene49569.t1 |
| Gene Sequence |
| Protein sequence |
