Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50265.t1 | XP_003605139.1 | 96.4 | 522 | 14 | 1 | 1 | 517 | 1 | 522 | 2.60E-282 | 980.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50265.t1 | P11043 | 76.4 | 521 | 111 | 5 | 4 | 517 | 1 | 516 | 7.0e-220 | 764.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50265.t1 | G7JEN4 | 96.4 | 522 | 14 | 1 | 1 | 517 | 1 | 522 | 1.9e-282 | 980.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053143 | MS.gene50265 | 0.8303 | 3.17E-55 | -1.69E-46 |
| MS.gene054490 | MS.gene50265 | 0.801166 | 1.02E-48 | -1.69E-46 |
| MS.gene056677 | MS.gene50265 | 0.820165 | 7.92E-53 | -1.69E-46 |
| MS.gene061100 | MS.gene50265 | 0.845016 | 5.28E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene50265 | MS.gene040883 | PPI |
| MS.gene50265 | MS.gene82992 | PPI |
| MS.gene040899 | MS.gene50265 | PPI |
| MS.gene50265 | MS.gene061004 | PPI |
| MS.gene50265 | MS.gene026451 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50265.t1 | MTR_4g024620 | 97.701 | 522 | 7 | 1 | 1 | 517 | 1 | 522 | 0.0 | 1040 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50265.t1 | AT2G45300 | 74.618 | 524 | 122 | 3 | 1 | 517 | 1 | 520 | 0.0 | 784 |
| MS.gene50265.t1 | AT2G45300 | 74.618 | 524 | 122 | 3 | 1 | 517 | 1 | 520 | 0.0 | 784 |
| MS.gene50265.t1 | AT2G45300 | 74.618 | 524 | 122 | 3 | 1 | 517 | 1 | 520 | 0.0 | 784 |
| MS.gene50265.t1 | AT2G45300 | 74.181 | 519 | 123 | 3 | 1 | 512 | 1 | 515 | 0.0 | 772 |
| MS.gene50265.t1 | AT1G48860 | 78.243 | 478 | 97 | 2 | 43 | 517 | 48 | 521 | 0.0 | 736 |
| MS.gene50265.t1 | AT1G48860 | 76.667 | 480 | 84 | 2 | 66 | 517 | 10 | 489 | 0.0 | 717 |
Find 134 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCCACCTGTTCGTATAAT+TGG | 0.119896 | 4.4:+77040854 | MS.gene50265:CDS |
| GAACCTATTAAGGAGATTTC+TGG | 0.215410 | 4.4:+77038373 | MS.gene50265:CDS |
| CTAGCTGGTGCAGCAGTTAC+TGG | 0.232551 | 4.4:+77041985 | MS.gene50265:CDS |
| GATTGTGTTGGAACCTATTA+AGG | 0.234545 | 4.4:+77038363 | MS.gene50265:CDS |
| TTAACTTCATCTTTAGATTC+AGG | 0.269585 | 4.4:-77040191 | None:intergenic |
| TTCATTGAGGTCAAGCATTT+GGG | 0.273349 | 4.4:+77038219 | MS.gene50265:CDS |
| GCCTTCCACAATAGCTTGTT+TGG | 0.300378 | 4.4:-77040153 | None:intergenic |
| TTGTTGAATTGTGAAAATTA+TGG | 0.304085 | 4.4:-77038165 | None:intergenic |
| CTACCAGAAATCTCCTTAAT+AGG | 0.310280 | 4.4:-77038376 | None:intergenic |
| TCTGGTAGTATTACATTGCC+TGG | 0.310752 | 4.4:+77038391 | MS.gene50265:CDS |
| TGCTAATTGGGACAGGTTCT+TGG | 0.312726 | 4.4:+77041807 | MS.gene50265:CDS |
| TTTCATTGAGGTCAAGCATT+TGG | 0.318418 | 4.4:+77038218 | MS.gene50265:CDS |
| ATTGGGAAGGGAGGGCTTCC+AGG | 0.321255 | 4.4:+77040872 | MS.gene50265:CDS |
| GTACTTGACTGCTTTGCTTA+TGG | 0.322664 | 4.4:+77041666 | MS.gene50265:CDS |
| GGGGAATTCTGTTGTGTTGA+AGG | 0.324609 | 4.4:+77038309 | MS.gene50265:CDS |
| GATGAAGTTAATTTATTCCT+TGG | 0.328404 | 4.4:+77040203 | MS.gene50265:CDS |
| TTCTGTTGCTGCTGCGGCGC+CGG | 0.329924 | 4.4:+77038339 | MS.gene50265:CDS |
| CGAAATGACTTTGAAGTTGA+TGG | 0.332647 | 4.4:+77041756 | MS.gene50265:CDS |
| TCGTAGAACACAGTGCTAAT+TGG | 0.345920 | 4.4:+77041794 | MS.gene50265:CDS |
| CAATTGGTCTCTCTCTCATT+CGG | 0.348009 | 4.4:-77040769 | None:intergenic |
| CCACATCACAATTTCCAATA+AGG | 0.351381 | 4.4:-77038282 | None:intergenic |
| CTGTGGGACAAGCAGTTTAC+AGG | 0.352564 | 4.4:+77042029 | MS.gene50265:CDS |
| GGCGGAACTATCACGGTTAT+AGG | 0.353223 | 4.4:+77042006 | MS.gene50265:CDS |
| CCAGGATCCTTGATGGTAAC+TGG | 0.359841 | 4.4:-77045541 | None:intergenic |
| ACCTTCAACAAAAGCATTGC+CGG | 0.362144 | 4.4:-77041938 | None:intergenic |
| TGGAAATTGTGATGTGGGTA+GGG | 0.366191 | 4.4:+77038288 | MS.gene50265:CDS |
| CGCATGCAGGTGAAACTGTC+TGG | 0.373379 | 4.4:+77041628 | MS.gene50265:intron |
| TGCTGAAGTTCTTGAAAAGA+TGG | 0.379657 | 4.4:+77043865 | MS.gene50265:CDS |
| GATGTGACATTCAATTTCTC+AGG | 0.381023 | 4.4:-77045454 | None:intergenic |
| GAAAACAGTGTAACTGTTAC+CGG | 0.381454 | 4.4:+77043908 | MS.gene50265:CDS |
| GGTCTAAGTCTTTGTCTAAT+CGG | 0.388353 | 4.4:+77038413 | MS.gene50265:CDS |
| TGTTGAATTGTGAAAATTAT+GGG | 0.390247 | 4.4:-77038164 | None:intergenic |
| CGTAGAACACAGTGCTAATT+GGG | 0.396858 | 4.4:+77041795 | MS.gene50265:CDS |
| GGAAATTGTGATGTGGGTAG+GGG | 0.402968 | 4.4:+77038289 | MS.gene50265:CDS |
| TCAAGGACTTGAAAGTAGTC+AGG | 0.407342 | 4.4:-77045583 | None:intergenic |
| AATTGGTCTCTCTCTCATTC+GGG | 0.412106 | 4.4:-77040768 | None:intergenic |
| CCCTTCCCAATTATACGAAC+AGG | 0.423334 | 4.4:-77040860 | None:intergenic |
| TACTGCAATGCGTCCTTTGA+CGG | 0.424822 | 4.4:+77040235 | MS.gene50265:CDS |
| ATGGCAGCTCCGTTGGCCCT+TGG | 0.427336 | 4.4:+77041685 | MS.gene50265:CDS |
| TTTGCTTATGGCAGCTCCGT+TGG | 0.432957 | 4.4:+77041678 | MS.gene50265:CDS |
| GCAAGTGTCATGGCAACATC+TGG | 0.434911 | 4.4:-77043995 | None:intergenic |
| ACGGCAGCCTTGGTTGCTGC+AGG | 0.440585 | 4.4:+77040254 | MS.gene50265:CDS |
| GGGCCTCCACGGGATTCTTC+CGG | 0.442182 | 4.4:+77043929 | MS.gene50265:CDS |
| CCAATTGGAGATTTAGTGTC+TGG | 0.444538 | 4.4:+77040785 | MS.gene50265:CDS |
| TTTGGGGCTCTTCGAAATCA+TGG | 0.446256 | 4.4:+77038236 | MS.gene50265:CDS |
| GTCCACCTGTTCGTATAATT+GGG | 0.447258 | 4.4:+77040855 | MS.gene50265:CDS |
| TTGAAGTTGATGGAGCGCTT+TGG | 0.447617 | 4.4:+77041766 | MS.gene50265:CDS |
| CGAATGAGAGAGAGACCAAT+TGG | 0.449698 | 4.4:+77040770 | MS.gene50265:CDS |
| GCGTCCTTTGACGGCAGCCT+TGG | 0.455283 | 4.4:+77040244 | MS.gene50265:CDS |
| TGTGCAACCAGGATCCTTGA+TGG | 0.456502 | 4.4:-77045548 | None:intergenic |
| GGTGTTATCACACAGTAATC+AGG | 0.461114 | 4.4:-77045430 | None:intergenic |
| CCAGACACTAAATCTCCAAT+TGG | 0.462623 | 4.4:-77040785 | None:intergenic |
| CAAAGGACGCATTGCAGTAC+CGG | 0.465602 | 4.4:-77040231 | None:intergenic |
| CTTGAGGCATTAAAGACCCT+TGG | 0.467801 | 4.4:+77040104 | MS.gene50265:CDS |
| TTGGAAATTGTGATGTGGGT+AGG | 0.467982 | 4.4:+77038287 | MS.gene50265:CDS |
| AACTGTTACCGGGCCTCCAC+GGG | 0.469893 | 4.4:+77043919 | MS.gene50265:CDS |
| AGTGCTAGTTACTTTCTAGC+TGG | 0.478748 | 4.4:+77041970 | MS.gene50265:CDS |
| CCAGTTACCATCAAGGATCC+TGG | 0.478835 | 4.4:+77045541 | MS.gene50265:CDS |
| TTCAAAGTCATTTCGACATA+CGG | 0.479092 | 4.4:-77041748 | None:intergenic |
| CTGGTAGTATTACATTGCCT+GGG | 0.479564 | 4.4:+77038392 | MS.gene50265:CDS |
| CAGCAAAATCAGTTTCATTG+AGG | 0.481774 | 4.4:+77038206 | MS.gene50265:CDS |
| GTTGTTGCACTATATGCTAA+TGG | 0.485600 | 4.4:+77044019 | MS.gene50265:CDS |
| AATTTATTCCTTGGAAATGC+CGG | 0.486192 | 4.4:+77040212 | MS.gene50265:CDS |
| TTCTGCTTCTGTTGCTGCTG+CGG | 0.486404 | 4.4:+77038333 | MS.gene50265:CDS |
| GGAAGAATCCCGTGGAGGCC+CGG | 0.497540 | 4.4:-77043927 | None:intergenic |
| TAACTGTTACCGGGCCTCCA+CGG | 0.500019 | 4.4:+77043918 | MS.gene50265:CDS |
| TGGGACAGGTTCTTGGTCCA+TGG | 0.500853 | 4.4:+77041814 | MS.gene50265:CDS |
| GCTGAAGTTCTTGAAAAGAT+GGG | 0.501702 | 4.4:+77043866 | MS.gene50265:CDS |
| AGAGAACTTGTTAGATAGTG+AGG | 0.501939 | 4.4:+77040067 | MS.gene50265:CDS |
| ATTTCAGATACATACTTGAC+GGG | 0.514182 | 4.4:+77040741 | MS.gene50265:intron |
| ACTATCACGGTTATAGGCTG+TGG | 0.515721 | 4.4:+77042012 | MS.gene50265:CDS |
| ATAGGTTCCAACACAATCTC+CGG | 0.517739 | 4.4:-77038358 | None:intergenic |
| TATTTCAGATACATACTTGA+CGG | 0.519370 | 4.4:+77040740 | MS.gene50265:intron |
| GCAACCAAGGCTGCCGTCAA+AGG | 0.525534 | 4.4:-77040248 | None:intergenic |
| AATGCTTTGTAAATCTCTCA+AGG | 0.535419 | 4.4:-77045600 | None:intergenic |
| TACATATGATGATCATAGAA+TGG | 0.536685 | 4.4:+77045483 | MS.gene50265:CDS |
| GCAGCCTTGGTTGCTGCAGG+TGG | 0.537285 | 4.4:+77040257 | MS.gene50265:CDS |
| CAAGCTATTGTGGAAGGCAG+TGG | 0.542344 | 4.4:+77040158 | MS.gene50265:CDS |
| TGGGAAGGGAGGGCTTCCAG+GGG | 0.543181 | 4.4:+77040874 | MS.gene50265:CDS |
| TTGGGAAGGGAGGGCTTCCA+GGG | 0.543947 | 4.4:+77040873 | MS.gene50265:CDS |
| GTGTCTGGTCTTAAGCAACT+TGG | 0.547127 | 4.4:+77040800 | MS.gene50265:CDS |
| TTGCTGCAGGTGGAAATACA+AGG | 0.547549 | 4.4:+77040267 | MS.gene50265:CDS |
| ACCTGTTCGTATAATTGGGA+AGG | 0.547613 | 4.4:+77040859 | MS.gene50265:CDS |
| GAAATTGTGATGTGGGTAGG+GGG | 0.552289 | 4.4:+77038290 | MS.gene50265:CDS |
| TTCGTATAATTGGGAAGGGA+GGG | 0.553161 | 4.4:+77040864 | MS.gene50265:CDS |
| TTGCAGTACCGGCATTTCCA+AGG | 0.558927 | 4.4:-77040220 | None:intergenic |
| CATCTTCCACACGAAGTCCA+AGG | 0.563075 | 4.4:-77040121 | None:intergenic |
| CAAGGACTTGAAAGTAGTCA+GGG | 0.567498 | 4.4:-77045582 | None:intergenic |
| AGATGGGAGCTAAAGTTACA+TGG | 0.567569 | 4.4:+77043882 | MS.gene50265:CDS |
| CCTTCAACAAAAGCATTGCC+GGG | 0.568002 | 4.4:-77041937 | None:intergenic |
| GCCTTGCAAGACTTTCCGAC+CGG | 0.570695 | 4.4:-77043948 | None:intergenic |
| AACACAGTGCTAATTGGGAC+AGG | 0.576861 | 4.4:+77041800 | MS.gene50265:CDS |
| TGATGATCATAGAATGGCCA+TGG | 0.577715 | 4.4:+77045489 | MS.gene50265:CDS |
| GGTCCCACTGCTATTAGAGA+TGG | 0.578119 | 4.4:+77044040 | MS.gene50265:CDS |
| AACAACCAAACAAGCTATTG+TGG | 0.581759 | 4.4:+77040148 | MS.gene50265:CDS |
| GGGAAGGGAGGGCTTCCAGG+GGG | 0.584040 | 4.4:+77040875 | MS.gene50265:CDS |
| TTAGACAAAGACTTAGACCC+AGG | 0.585624 | 4.4:-77038409 | None:intergenic |
| ATCTTCCACACGAAGTCCAA+GGG | 0.589211 | 4.4:-77040120 | None:intergenic |
| GTTCGTATAATTGGGAAGGG+AGG | 0.589281 | 4.4:+77040863 | MS.gene50265:CDS |
| GCTTCTTGCTGCTCTCTCTG+AGG | 0.591041 | 4.4:+77038441 | MS.gene50265:CDS |
| GCTATTGTGGAAGGCAGTGG+TGG | 0.591807 | 4.4:+77040161 | MS.gene50265:CDS |
| CTTATTGGAAATTGTGATGT+GGG | 0.592238 | 4.4:+77038283 | MS.gene50265:CDS |
| CTAGGAGCAATAGTTGAAGA+AGG | 0.594639 | 4.4:+77045406 | MS.gene50265:CDS |
| GGATATTCATTACATGCTTG+AGG | 0.596449 | 4.4:+77040088 | MS.gene50265:CDS |
| ATTTCCACCTGCAGCAACCA+AGG | 0.596983 | 4.4:-77040261 | None:intergenic |
| AAAGACCCTTGGACTTCGTG+TGG | 0.599980 | 4.4:+77040115 | MS.gene50265:CDS |
| TCCGGTCGGAAAGTCTTGCA+AGG | 0.600579 | 4.4:+77043947 | MS.gene50265:CDS |
| GACAGGTTCTTGGTCCATGG+AGG | 0.603780 | 4.4:+77041817 | MS.gene50265:CDS |
| AGTTACTGGCGGAACTATCA+CGG | 0.604333 | 4.4:+77041999 | MS.gene50265:CDS |
| TGCAACAACAGCAAGTGTCA+TGG | 0.606229 | 4.4:-77044005 | None:intergenic |
| AGCAGCAAGTGAAAATGCCA+TGG | 0.609806 | 4.4:-77045506 | None:intergenic |
| CCTTATTGGAAATTGTGATG+TGG | 0.610485 | 4.4:+77038282 | MS.gene50265:CDS |
| TGATGTTCCAGTTACCATCA+AGG | 0.610902 | 4.4:+77045534 | MS.gene50265:CDS |
| CTATCACGGTTATAGGCTGT+GGG | 0.615693 | 4.4:+77042013 | MS.gene50265:CDS |
| TCAAGGATCCTGGTTGCACA+AGG | 0.616615 | 4.4:+77045551 | MS.gene50265:CDS |
| ACCAAACAAGCTATTGTGGA+AGG | 0.616937 | 4.4:+77040152 | MS.gene50265:CDS |
| CTCAATCTCAACATCACCAA+GGG | 0.617444 | 4.4:-77041701 | None:intergenic |
| CCTGTTCGTATAATTGGGAA+GGG | 0.621907 | 4.4:+77040860 | MS.gene50265:CDS |
| AATCTGCACAGAACTCAGAA+AGG | 0.627914 | 4.4:+77045156 | MS.gene50265:CDS |
| TTCCCAATTATACGAACAGG+TGG | 0.629982 | 4.4:-77040857 | None:intergenic |
| TGCGGCGCCGGAGATTGTGT+TGG | 0.631894 | 4.4:+77038351 | MS.gene50265:CDS |
| TCAACATCACCAAGGGCCAA+CGG | 0.634886 | 4.4:-77041694 | None:intergenic |
| CTCCACGGGATTCTTCCGGT+CGG | 0.638233 | 4.4:+77043933 | MS.gene50265:CDS |
| GTGACATTCAATTTCTCAGG+TGG | 0.643818 | 4.4:-77045451 | None:intergenic |
| ATACCATCTCTAATAGCAGT+GGG | 0.648802 | 4.4:-77044043 | None:intergenic |
| GCTGGTGCAGCAGTTACTGG+CGG | 0.649225 | 4.4:+77041988 | MS.gene50265:CDS |
| CATACCATCTCTAATAGCAG+TGG | 0.651100 | 4.4:-77044044 | None:intergenic |
| TCATTGAGGTCAAGCATTTG+GGG | 0.651769 | 4.4:+77038220 | MS.gene50265:CDS |
| ATTGGTCTCTCTCTCATTCG+GGG | 0.653517 | 4.4:-77040767 | None:intergenic |
| TTCCGACCGGAAGAATCCCG+TGG | 0.664740 | 4.4:-77043935 | None:intergenic |
| AAAACAGTGTAACTGTTACC+GGG | 0.672809 | 4.4:+77043909 | MS.gene50265:CDS |
| TCTCAATCTCAACATCACCA+AGG | 0.684401 | 4.4:-77041702 | None:intergenic |
| TTTCAGATACATACTTGACG+GGG | 0.713014 | 4.4:+77040742 | MS.gene50265:intron |
| CGACCGGAAGAATCCCGTGG+AGG | 0.725610 | 4.4:-77043932 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 77038124 | 77045624 | 77038124 | ID=MS.gene50265 |
| chr4.4 | mRNA | 77038124 | 77045624 | 77038124 | ID=MS.gene50265.t1;Parent=MS.gene50265 |
| chr4.4 | exon | 77038124 | 77038462 | 77038124 | ID=MS.gene50265.t1.exon1;Parent=MS.gene50265.t1 |
| chr4.4 | CDS | 77038124 | 77038462 | 77038124 | ID=cds.MS.gene50265.t1;Parent=MS.gene50265.t1 |
| chr4.4 | exon | 77040053 | 77040288 | 77040053 | ID=MS.gene50265.t1.exon2;Parent=MS.gene50265.t1 |
| chr4.4 | CDS | 77040053 | 77040288 | 77040053 | ID=cds.MS.gene50265.t1;Parent=MS.gene50265.t1 |
| chr4.4 | exon | 77040748 | 77040901 | 77040748 | ID=MS.gene50265.t1.exon3;Parent=MS.gene50265.t1 |
| chr4.4 | CDS | 77040748 | 77040901 | 77040748 | ID=cds.MS.gene50265.t1;Parent=MS.gene50265.t1 |
| chr4.4 | exon | 77041637 | 77041851 | 77041637 | ID=MS.gene50265.t1.exon4;Parent=MS.gene50265.t1 |
| chr4.4 | CDS | 77041637 | 77041851 | 77041637 | ID=cds.MS.gene50265.t1;Parent=MS.gene50265.t1 |
| chr4.4 | exon | 77041933 | 77042050 | 77041933 | ID=MS.gene50265.t1.exon5;Parent=MS.gene50265.t1 |
| chr4.4 | CDS | 77041933 | 77042050 | 77041933 | ID=cds.MS.gene50265.t1;Parent=MS.gene50265.t1 |
| chr4.4 | exon | 77043851 | 77044061 | 77043851 | ID=MS.gene50265.t1.exon6;Parent=MS.gene50265.t1 |
| chr4.4 | CDS | 77043851 | 77044061 | 77043851 | ID=cds.MS.gene50265.t1;Parent=MS.gene50265.t1 |
| chr4.4 | exon | 77045116 | 77045177 | 77045116 | ID=MS.gene50265.t1.exon7;Parent=MS.gene50265.t1 |
| chr4.4 | CDS | 77045116 | 77045177 | 77045116 | ID=cds.MS.gene50265.t1;Parent=MS.gene50265.t1 |
| chr4.4 | exon | 77045406 | 77045624 | 77045406 | ID=MS.gene50265.t1.exon8;Parent=MS.gene50265.t1 |
| chr4.4 | CDS | 77045406 | 77045624 | 77045406 | ID=cds.MS.gene50265.t1;Parent=MS.gene50265.t1 |
| Gene Sequence |
| Protein sequence |
