Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50269.t1 | XP_003605133.2 | 100 | 119 | 0 | 0 | 4 | 122 | 33 | 151 | 1.10E-58 | 235.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50269.t1 | P46298 | 97.5 | 119 | 3 | 0 | 4 | 122 | 33 | 151 | 2.5e-59 | 229.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50269.t1 | B7FGJ7 | 100.0 | 119 | 0 | 0 | 4 | 122 | 33 | 151 | 8.0e-59 | 235.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050134 | MS.gene50269 | 0.829284 | 5.61E-55 | -1.69E-46 |
| MS.gene052664 | MS.gene50269 | 0.850979 | 1.20E-60 | -1.69E-46 |
| MS.gene053224 | MS.gene50269 | 0.848064 | 7.78E-60 | -1.69E-46 |
| MS.gene057643 | MS.gene50269 | 0.818162 | 2.26E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50269.t1 | MTR_4g024550 | 100.000 | 119 | 0 | 0 | 4 | 122 | 33 | 151 | 1.14e-83 | 241 |
| MS.gene50269.t1 | MTR_5g011910 | 98.319 | 119 | 2 | 0 | 4 | 122 | 33 | 151 | 2.27e-82 | 238 |
| MS.gene50269.t1 | MTR_4g102170 | 96.639 | 119 | 4 | 0 | 4 | 122 | 33 | 151 | 7.19e-81 | 234 |
| MS.gene50269.t1 | MTR_4g024265 | 100.000 | 81 | 0 | 0 | 4 | 84 | 33 | 113 | 4.63e-53 | 171 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50269.t1 | AT4G00100 | 95.798 | 119 | 5 | 0 | 4 | 122 | 33 | 151 | 1.23e-80 | 233 |
| MS.gene50269.t1 | AT3G60770 | 95.798 | 119 | 5 | 0 | 4 | 122 | 33 | 151 | 2.09e-80 | 233 |
Find 32 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCAAATTCAGGCTAATTT+TGG | 0.149134 | 4.4:-77153090 | MS.gene50269:CDS |
| GACCAAAATTAGCCTGAATT+TGG | 0.223891 | 4.4:+77153088 | None:intergenic |
| TATGCGCAAAATCTTGTTTC+CGG | 0.257809 | 4.4:+77153329 | None:intergenic |
| AGATGGAGTTAGACCTTTCT+TGG | 0.271027 | 4.4:+77153407 | None:intergenic |
| TACAGATCTTCAGGAATTTC+AGG | 0.309178 | 4.4:+77153176 | None:intergenic |
| ATCAGGTGATACAGATCTTC+AGG | 0.338551 | 4.4:+77153167 | None:intergenic |
| CAAAGAAGCTTCCACCAGTA+TGG | 0.349985 | 4.4:-77153025 | MS.gene50269:CDS |
| GGTCTAACTCCATCTCAAAT+TGG | 0.359176 | 4.4:-77153399 | MS.gene50269:CDS |
| GGAAGCATTTGGAGAGGAAC+AGG | 0.374872 | 4.4:-77153124 | MS.gene50269:CDS |
| TCAAGAAGGCTGTCTCAATT+AGG | 0.379771 | 4.4:-77153145 | MS.gene50269:CDS |
| AATACTTACTATTTCCATAC+TGG | 0.381602 | 4.4:+77153011 | None:intergenic |
| TTGAGACAGCCTTCTTGATC+AGG | 0.385210 | 4.4:+77153150 | None:intergenic |
| CCATGGAATTGCTCAGGTTA+AGG | 0.405652 | 4.4:-77153358 | MS.gene50269:CDS |
| TGTCTCAATTAGGAAGCATT+TGG | 0.438327 | 4.4:-77153135 | MS.gene50269:CDS |
| AGGACAAGGACTCCAAATTC+AGG | 0.445955 | 4.4:-77153100 | MS.gene50269:CDS |
| ATCTGCAAGTTTGCCAAGAA+AGG | 0.457907 | 4.4:-77153420 | MS.gene50269:CDS |
| GCTCAGGTTAAGGCTGTAAC+CGG | 0.462422 | 4.4:-77153348 | MS.gene50269:CDS |
| TGACTCCCATGGAATTGCTC+AGG | 0.496575 | 4.4:-77153364 | MS.gene50269:CDS |
| GTTATTCTTCGTGACTCCCA+TGG | 0.508409 | 4.4:-77153375 | MS.gene50269:CDS |
| CCTTAACCTGAGCAATTCCA+TGG | 0.520963 | 4.4:+77153358 | None:intergenic |
| TTTCTTGGCAAACTTGCAGA+TGG | 0.531932 | 4.4:+77153422 | None:intergenic |
| TCTTCAAGCAACAAGAGTGC+TGG | 0.541036 | 4.4:+77152425 | None:intergenic |
| ACTTACTATTTCCATACTGG+TGG | 0.562660 | 4.4:+77153014 | None:intergenic |
| TATAGTAACGAGCAAGGCGA+TGG | 0.565886 | 4.4:+77153054 | None:intergenic |
| AGAATAACACCAATTTGAGA+TGG | 0.576179 | 4.4:+77153390 | None:intergenic |
| TCTTCTTATAGTAACGAGCA+AGG | 0.583518 | 4.4:+77153048 | None:intergenic |
| TCTGTATCACCTGATCAAGA+AGG | 0.601322 | 4.4:-77153159 | MS.gene50269:CDS |
| TTGCGCATATTGAAGGCGCA+TGG | 0.612509 | 4.4:-77153315 | MS.gene50269:intron |
| CAATTAGGAAGCATTTGGAG+AGG | 0.640013 | 4.4:-77153130 | MS.gene50269:CDS |
| CTTAACCTGAGCAATTCCAT+GGG | 0.650971 | 4.4:+77153359 | None:intergenic |
| GGAGAGGAACAGGAAGGACA+AGG | 0.660004 | 4.4:-77153114 | MS.gene50269:CDS |
| GCATTTGGAGAGGAACAGGA+AGG | 0.678718 | 4.4:-77153120 | MS.gene50269:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTATCGATTTATT+AGG | - | chr4.4:77153358-77153377 | MS.gene50269:CDS | 10.0% |
| !! | AACATTATAAAGATAAAATG+TGG | + | chr4.4:77153668-77153687 | None:intergenic | 15.0% |
| !! | TATCATTAATATCTTCTTAT+AGG | + | chr4.4:77152538-77152557 | None:intergenic | 15.0% |
| !!! | ATCTCATAAGTAATTATTTT+AGG | - | chr4.4:77153568-77153587 | MS.gene50269:intron | 15.0% |
| !!! | ACTGAATTTTTTTATTTGCT+TGG | - | chr4.4:77153401-77153420 | MS.gene50269:CDS | 20.0% |
| !!! | ATTTAAAACCAAGAAAATTC+AGG | + | chr4.4:77153436-77153455 | None:intergenic | 20.0% |
| !!! | GTATTCTCTATCTTAATTTT+TGG | - | chr4.4:77153710-77153729 | MS.gene50269:intron | 20.0% |
| !!! | TAAATTTTTGTTCCTGATTA+AGG | - | chr4.4:77153802-77153821 | MS.gene50269:intron | 20.0% |
| !!! | TTTTGTCAATTTTCGTTTTT+TGG | + | chr4.4:77152731-77152750 | None:intergenic | 20.0% |
| ! | AACAATGATATATTCCTGTT+TGG | + | chr4.4:77153599-77153618 | None:intergenic | 25.0% |
| ! | AATACTTACTATTTCCATAC+TGG | + | chr4.4:77153311-77153330 | None:intergenic | 25.0% |
| ! | ATTAGAAATGAGATGCAAAA+TGG | + | chr4.4:77153833-77153852 | None:intergenic | 25.0% |
| ! | TAACAGTATAACACTTAACA+TGG | - | chr4.4:77152432-77152451 | MS.gene50269:CDS | 25.0% |
| ! | TCTCATTTCTAATACTTGTT+TGG | - | chr4.4:77153840-77153859 | MS.gene50269:intron | 25.0% |
| !! | AAGTTCATTAGCTTATAGAA+AGG | + | chr4.4:77153497-77153516 | None:intergenic | 25.0% |
| !!! | AAGCTTGTTGAAATAAGTTT+AGG | - | chr4.4:77153543-77153562 | MS.gene50269:intron | 25.0% |
| !!! | CTTTAATTTCTCTTTTCCTA+GGG | - | chr4.4:77153114-77153133 | MS.gene50269:CDS | 25.0% |
| !!! | TCTTTAATTTCTCTTTTCCT+AGG | - | chr4.4:77153113-77153132 | MS.gene50269:CDS | 25.0% |
| !!! | TTTTATTTGCTTGGTTTTGA+AGG | - | chr4.4:77153410-77153429 | MS.gene50269:CDS | 25.0% |
| AAAATGGCAATTCCTTAATC+AGG | + | chr4.4:77153817-77153836 | None:intergenic | 30.0% | |
| AAATGAAGTAACATGTTGCA+AGG | + | chr4.4:77153746-77153765 | None:intergenic | 30.0% | |
| AGAATAACACCAATTTGAGA+TGG | + | chr4.4:77152932-77152951 | None:intergenic | 30.0% | |
| CATTAAAAAAGGTCTGCAAT+TGG | - | chr4.4:77152468-77152487 | MS.gene50269:intron | 30.0% | |
| GGTTGTTTAAACTTATCTCA+TGG | + | chr4.4:77153624-77153643 | None:intergenic | 30.0% | |
| GTGTTTACAGATCATAAAAG+TGG | + | chr4.4:77152785-77152804 | None:intergenic | 30.0% | |
| ! | AGTGCTTGTAAGCTATATTT+TGG | - | chr4.4:77153520-77153539 | MS.gene50269:intron | 30.0% |
| ! | CCTAGTTAATCTTTTAGATC+CGG | + | chr4.4:77152576-77152595 | None:intergenic | 30.0% |
| ! | CTTTTATGATCTGTAAACAC+TGG | - | chr4.4:77152785-77152804 | MS.gene50269:intron | 30.0% |
| !! | TATTTTAGGTAGATCCAAAC+AGG | - | chr4.4:77153582-77153601 | MS.gene50269:intron | 30.0% |
| !! | TTAGGCTTGATTAATTTGTG+AGG | - | chr4.4:77153376-77153395 | MS.gene50269:CDS | 30.0% |
| ACTTACTATTTCCATACTGG+TGG | + | chr4.4:77153308-77153327 | None:intergenic | 35.0% | |
| ATCATCGCATCAAACATGAT+TGG | + | chr4.4:77152618-77152637 | None:intergenic | 35.0% | |
| CCGGATCTAAAAGATTAACT+AGG | - | chr4.4:77152573-77152592 | MS.gene50269:intron | 35.0% | |
| CTGAATTATTGTTGTGATGC+AGG | + | chr4.4:77152660-77152679 | None:intergenic | 35.0% | |
| CTTAATAATGCATAGAGCAG+AGG | + | chr4.4:77153645-77153664 | None:intergenic | 35.0% | |
| GATAATAGTCGATGAACAAC+CGG | - | chr4.4:77152554-77152573 | MS.gene50269:intron | 35.0% | |
| TACAGATCTTCAGGAATTTC+AGG | + | chr4.4:77153146-77153165 | None:intergenic | 35.0% | |
| TCTTCTTATAGTAACGAGCA+AGG | + | chr4.4:77153274-77153293 | None:intergenic | 35.0% | |
| ! | CAAGATTTTGCGCATATTGA+AGG | - | chr4.4:77152997-77153016 | MS.gene50269:intron | 35.0% |
| ! | CTCCAAATTCAGGCTAATTT+TGG | - | chr4.4:77153229-77153248 | MS.gene50269:intron | 35.0% |
| ! | GACCAAAATTAGCCTGAATT+TGG | + | chr4.4:77153234-77153253 | None:intergenic | 35.0% |
| ! | TATGCGCAAAATCTTGTTTC+CGG | + | chr4.4:77152993-77153012 | None:intergenic | 35.0% |
| ! | TTTGAAGGCCTGAATTTTCT+TGG | - | chr4.4:77153425-77153444 | MS.gene50269:CDS | 35.0% |
| !! | TGTCTCAATTAGGAAGCATT+TGG | - | chr4.4:77153184-77153203 | MS.gene50269:CDS | 35.0% |
| AGATGGAGTTAGACCTTTCT+TGG | + | chr4.4:77152915-77152934 | None:intergenic | 40.0% | |
| ATCAGGTGATACAGATCTTC+AGG | + | chr4.4:77153155-77153174 | None:intergenic | 40.0% | |
| ATCTGCAAGTTTGCCAAGAA+AGG | - | chr4.4:77152899-77152918 | MS.gene50269:intron | 40.0% | |
| CTGATGTTCATGAATTGCAC+AGG | - | chr4.4:77152865-77152884 | MS.gene50269:intron | 40.0% | |
| CTTAACCTGAGCAATTCCAT+GGG | + | chr4.4:77152963-77152982 | None:intergenic | 40.0% | |
| GGTCTAACTCCATCTCAAAT+TGG | - | chr4.4:77152920-77152939 | MS.gene50269:intron | 40.0% | |
| TCAAGAAGGCTGTCTCAATT+AGG | - | chr4.4:77153174-77153193 | MS.gene50269:CDS | 40.0% | |
| TCTGTATCACCTGATCAAGA+AGG | - | chr4.4:77153160-77153179 | MS.gene50269:CDS | 40.0% | |
| TTTCTTGGCAAACTTGCAGA+TGG | + | chr4.4:77152900-77152919 | None:intergenic | 40.0% | |
| ! | GTGATGCAGGACAACATTTT+CGG | + | chr4.4:77152647-77152666 | None:intergenic | 40.0% |
| !! | CAATTAGGAAGCATTTGGAG+AGG | - | chr4.4:77153189-77153208 | MS.gene50269:intron | 40.0% |
| !! | TTGTTGATTCGCTGCAATAG+TGG | + | chr4.4:77153871-77153890 | None:intergenic | 40.0% |
| AGGACAAGGACTCCAAATTC+AGG | - | chr4.4:77153219-77153238 | MS.gene50269:intron | 45.0% | |
| CAAAGAAGCTTCCACCAGTA+TGG | - | chr4.4:77153294-77153313 | MS.gene50269:intron | 45.0% | |
| CCATGGAATTGCTCAGGTTA+AGG | - | chr4.4:77152961-77152980 | MS.gene50269:intron | 45.0% | |
| CCTTAACCTGAGCAATTCCA+TGG | + | chr4.4:77152964-77152983 | None:intergenic | 45.0% | |
| GTTATTCTTCGTGACTCCCA+TGG | - | chr4.4:77152944-77152963 | MS.gene50269:intron | 45.0% | |
| TATAGTAACGAGCAAGGCGA+TGG | + | chr4.4:77153268-77153287 | None:intergenic | 45.0% | |
| TTGAGACAGCCTTCTTGATC+AGG | + | chr4.4:77153172-77153191 | None:intergenic | 45.0% | |
| !!! | TTTTTTTTTTTCATTAAAAA+AGG | - | chr4.4:77152457-77152476 | MS.gene50269:intron | 5.0% |
| GAATTTCAGGAGCAAGCCCT+AGG | + | chr4.4:77153133-77153152 | None:intergenic | 50.0% | |
| GCATTTGGAGAGGAACAGGA+AGG | - | chr4.4:77153199-77153218 | MS.gene50269:intron | 50.0% | |
| GCTCAGGTTAAGGCTGTAAC+CGG | - | chr4.4:77152971-77152990 | MS.gene50269:intron | 50.0% | |
| TGACTCCCATGGAATTGCTC+AGG | - | chr4.4:77152955-77152974 | MS.gene50269:intron | 50.0% | |
| ! | TTGCGCATATTGAAGGCGCA+TGG | - | chr4.4:77153004-77153023 | MS.gene50269:intron | 50.0% |
| !! | GGAAGCATTTGGAGAGGAAC+AGG | - | chr4.4:77153195-77153214 | MS.gene50269:intron | 50.0% |
| !! | GGCTAATTTTGGTCGAGAGC+AGG | - | chr4.4:77153240-77153259 | MS.gene50269:intron | 50.0% |
| GGAGAGGAACAGGAAGGACA+AGG | - | chr4.4:77153205-77153224 | MS.gene50269:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 77152428 | 77153913 | 77152428 | ID=MS.gene50269 |
| chr4.4 | mRNA | 77152428 | 77153913 | 77152428 | ID=MS.gene50269.t1;Parent=MS.gene50269 |
| chr4.4 | exon | 77153905 | 77153913 | 77153905 | ID=MS.gene50269.t1.exon1;Parent=MS.gene50269.t1 |
| chr4.4 | CDS | 77153905 | 77153913 | 77153905 | ID=cds.MS.gene50269.t1;Parent=MS.gene50269.t1 |
| chr4.4 | exon | 77153316 | 77153454 | 77153316 | ID=MS.gene50269.t1.exon2;Parent=MS.gene50269.t1 |
| chr4.4 | CDS | 77153316 | 77153454 | 77153316 | ID=cds.MS.gene50269.t1;Parent=MS.gene50269.t1 |
| chr4.4 | exon | 77153020 | 77153206 | 77153020 | ID=MS.gene50269.t1.exon3;Parent=MS.gene50269.t1 |
| chr4.4 | CDS | 77153020 | 77153206 | 77153020 | ID=cds.MS.gene50269.t1;Parent=MS.gene50269.t1 |
| chr4.4 | exon | 77152428 | 77152461 | 77152428 | ID=MS.gene50269.t1.exon4;Parent=MS.gene50269.t1 |
| chr4.4 | CDS | 77152428 | 77152461 | 77152428 | ID=cds.MS.gene50269.t1;Parent=MS.gene50269.t1 |
| Gene Sequence |
| Protein sequence |
