AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50751

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50751.t1 MTR_4g070720 97.345 565 15 0 1 565 1 565 0.0 1150
MS.gene50751.t1 MTR_3g117090 71.750 577 143 12 1 565 1 569 0.0 768
MS.gene50751.t1 MTR_3g117090 71.715 548 138 11 1 539 1 540 0.0 743
MS.gene50751.t1 MTR_6g015645 70.179 446 114 10 133 565 9 448 0.0 594
MS.gene50751.t1 MTR_8g076880 58.094 556 203 11 26 565 13 554 0.0 590
MS.gene50751.t1 MTR_3g117100 54.500 600 216 16 19 565 28 623 0.0 583
MS.gene50751.t1 MTR_1g022480 52.749 582 225 13 24 560 35 611 0.0 559
MS.gene50751.t1 MTR_1g022480 52.568 584 225 13 24 560 35 613 0.0 558
MS.gene50751.t1 MTR_4g070810 54.196 572 211 13 42 565 48 616 0.0 555
MS.gene50751.t1 MTR_1g022480 52.122 589 225 14 24 560 35 618 0.0 551
MS.gene50751.t1 MTR_5g021050 74.085 328 82 3 32 356 10 337 5.51e-180 516
MS.gene50751.t1 MTR_5g021050 42.169 83 47 1 480 561 388 470 1.44e-14 76.6
MS.gene50751.t1 MTR_5g021050 74.085 328 82 3 32 356 10 337 1.23e-179 515
MS.gene50751.t1 MTR_5g021050 42.169 83 47 1 480 561 388 470 1.41e-14 76.6
MS.gene50751.t1 MTR_3g112150 49.914 583 231 10 21 565 13 572 5.38e-176 509
MS.gene50751.t1 MTR_1g022480 51.992 502 196 11 99 560 31 527 1.86e-164 479
MS.gene50751.t1 MTR_7g056587 57.057 333 134 3 12 337 11 341 8.46e-135 405
MS.gene50751.t1 MTR_7g056587 57.057 333 134 3 12 337 19 349 1.11e-134 405
MS.gene50751.t1 MTR_7g056587 57.057 333 134 3 12 337 19 349 1.75e-134 404
MS.gene50751.t1 MTR_7g056587 57.057 333 134 3 12 337 33 363 2.99e-134 404
MS.gene50751.t1 MTR_4g070790 57.616 302 115 3 68 362 49 344 4.91e-125 373
MS.gene50751.t1 MTR_3g010190 43.918 485 232 13 16 465 16 495 3.24e-124 375
MS.gene50751.t1 MTR_8g071000 39.206 579 291 15 38 563 33 603 1.04e-118 364
MS.gene50751.t1 MTR_5g012460 43.990 416 215 6 15 416 28 439 3.45e-112 342
MS.gene50751.t1 MTR_7g056587 64.732 224 79 0 114 337 1 224 4.97e-111 340
MS.gene50751.t1 MTR_4g070780 46.110 347 157 5 74 416 36 356 1.43e-92 290
MS.gene50751.t1 MTR_4g070730 52.985 268 87 4 73 339 41 270 8.80e-91 285
MS.gene50751.t1 MTR_6g034285 53.922 102 47 0 132 233 195 296 2.94e-29 120
MS.gene50751.t1 MTR_3g098530 31.687 243 146 8 70 311 44 267 6.00e-21 93.2
MS.gene50751.t1 MTR_3g060670 29.412 221 125 5 96 303 33 235 2.74e-20 92.8
MS.gene50751.t1 MTR_2g027140 28.270 237 136 6 83 303 23 241 4.81e-20 92.0
MS.gene50751.t1 MTR_8g096180 32.143 224 133 7 89 306 42 252 1.31e-19 89.4
MS.gene50751.t1 MTR_8g096180 31.963 219 130 7 89 306 42 242 2.16e-19 88.6
MS.gene50751.t1 MTR_8g096170 32.877 219 128 7 89 306 45 245 3.50e-19 87.8
MS.gene50751.t1 MTR_7g070480 24.747 396 220 15 27 389 30 380 1.34e-18 89.0
MS.gene50751.t1 MTR_4g013300 27.876 226 140 7 96 312 125 336 5.22e-16 80.9
MS.gene50751.t1 MTR_8g095360 26.754 228 148 6 96 316 181 396 1.28e-15 80.1
MS.gene50751.t1 MTR_2g027140 29.082 196 123 4 114 303 1 186 3.23e-15 76.6
MS.gene50751.t1 MTR_1g032580 29.577 213 131 8 97 306 32 228 1.91e-13 70.9
MS.gene50751.t1 MTR_1g022185 30.047 213 123 9 99 306 48 239 3.05e-13 70.9
MS.gene50751.t1 MTR_3g097350 27.074 229 148 7 87 306 238 456 5.22e-11 65.9
MS.gene50751.t1 MTR_2g031610 26.087 230 149 8 86 306 132 349 6.77e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50751.t1 AT2G21540 61.863 569 188 10 1 563 1 546 0.0 674
MS.gene50751.t1 AT2G21540 61.863 569 188 10 1 563 1 546 0.0 674
MS.gene50751.t1 AT2G21540 61.863 569 188 10 1 563 1 546 0.0 674
MS.gene50751.t1 AT2G21540 61.863 569 188 10 1 563 1 546 0.0 674
MS.gene50751.t1 AT2G21540 61.796 568 184 11 1 563 1 540 0.0 665
MS.gene50751.t1 AT2G21540 61.796 568 184 11 1 563 1 540 0.0 665
MS.gene50751.t1 AT4G36490 60.940 553 185 9 26 565 8 542 0.0 651
MS.gene50751.t1 AT4G39180 58.304 566 200 13 1 554 1 542 0.0 646
MS.gene50751.t1 AT4G39180 58.304 566 199 14 1 554 1 541 0.0 642
MS.gene50751.t1 AT4G39180 58.304 566 199 14 1 554 1 541 0.0 642
MS.gene50751.t1 AT2G18180 59.528 551 193 9 27 565 13 545 0.0 629
MS.gene50751.t1 AT2G18180 59.165 551 195 9 27 565 13 545 0.0 624
MS.gene50751.t1 AT4G34580 58.750 560 201 13 19 563 7 551 0.0 614
MS.gene50751.t1 AT4G34580 58.750 560 201 13 19 563 7 551 0.0 614
MS.gene50751.t1 AT4G39170 55.143 593 210 13 19 563 28 612 0.0 593
MS.gene50751.t1 AT4G39170 54.975 593 209 14 19 563 28 610 0.0 585
MS.gene50751.t1 AT2G21520 53.130 591 224 15 19 558 32 620 0.0 572
MS.gene50751.t1 AT2G21520 53.130 591 224 15 19 558 32 620 0.0 572
MS.gene50751.t1 AT2G21520 53.130 591 224 15 19 558 28 616 0.0 572
MS.gene50751.t1 AT2G18180 59.959 482 162 8 97 565 11 474 0.0 563
MS.gene50751.t1 AT2G16380 52.737 548 229 10 27 563 16 544 0.0 558
MS.gene50751.t1 AT2G16380 52.737 548 229 10 27 563 16 544 0.0 558
MS.gene50751.t1 AT2G18180 59.544 482 164 8 97 565 11 474 0.0 556
MS.gene50751.t1 AT2G18180 59.740 462 161 7 114 565 1 447 0.0 536
MS.gene50751.t1 AT1G19650 48.229 593 252 13 19 565 25 608 6.72e-175 508
MS.gene50751.t1 AT2G16380 52.183 458 192 8 114 563 1 439 1.33e-159 463
MS.gene50751.t1 AT1G19650 68.405 326 99 3 19 341 25 349 5.30e-159 461
MS.gene50751.t1 AT1G19650 48.256 516 216 10 93 565 29 536 3.07e-155 455
MS.gene50751.t1 AT3G24840 46.126 555 247 12 20 549 22 549 1.76e-154 455
MS.gene50751.t1 AT3G24840 46.126 555 247 12 20 549 22 549 1.76e-154 455
MS.gene50751.t1 AT3G24840 46.126 555 247 12 20 549 22 549 1.76e-154 455
MS.gene50751.t1 AT3G24840 46.126 555 247 12 20 549 22 549 1.76e-154 455
MS.gene50751.t1 AT3G24840 46.043 556 247 12 20 549 22 550 3.45e-154 454
MS.gene50751.t1 AT3G24840 46.043 556 247 12 20 549 22 550 3.45e-154 454
MS.gene50751.t1 AT3G24840 46.043 556 247 12 20 549 22 550 3.45e-154 454
MS.gene50751.t1 AT1G75370 67.964 334 99 2 19 344 28 361 5.28e-150 444
MS.gene50751.t1 AT1G75370 67.964 334 99 2 19 344 28 361 3.80e-149 444
MS.gene50751.t1 AT5G56160 38.544 563 319 10 21 561 15 572 7.74e-127 384
MS.gene50751.t1 AT5G56160 38.544 563 319 10 21 561 15 572 7.74e-127 384
MS.gene50751.t1 AT5G56160 54.180 323 143 4 21 338 15 337 1.39e-124 375
MS.gene50751.t1 AT1G55690 56.156 333 139 6 19 344 19 351 4.95e-122 373
MS.gene50751.t1 AT1G55690 56.156 333 139 6 19 344 19 351 8.35e-122 372
MS.gene50751.t1 AT1G55690 56.156 333 139 6 19 344 19 351 8.35e-122 372
MS.gene50751.t1 AT1G55690 56.156 333 139 6 19 344 19 351 8.35e-122 372
MS.gene50751.t1 AT1G55690 56.156 333 139 6 19 344 19 351 8.35e-122 372
MS.gene50751.t1 AT5G47510 51.331 263 128 0 72 334 27 289 4.31e-104 317
MS.gene50751.t1 AT5G47510 51.331 263 128 0 72 334 27 289 8.24e-104 318
MS.gene50751.t1 AT5G47510 51.331 263 128 0 72 334 26 288 9.59e-104 318
MS.gene50751.t1 AT5G47510 53.540 226 105 0 104 329 2 227 1.16e-92 287
MS.gene50751.t1 AT5G47730 28.631 241 157 5 72 303 7 241 9.39e-22 97.1
MS.gene50751.t1 AT5G47730 28.631 241 157 5 72 303 7 241 9.39e-22 97.1
MS.gene50751.t1 AT1G55840 28.230 209 142 3 97 303 12 214 3.72e-19 88.6
MS.gene50751.t1 AT1G55840 28.230 209 142 3 97 303 39 241 4.29e-19 89.0
MS.gene50751.t1 AT1G01630 29.644 253 151 9 57 306 17 245 3.84e-18 84.7
MS.gene50751.t1 AT5G47730 28.646 192 129 3 114 303 1 186 3.37e-17 82.8
MS.gene50751.t1 AT5G47730 28.646 192 129 3 114 303 1 186 3.37e-17 82.8
MS.gene50751.t1 AT1G72160 25.098 255 164 8 72 310 136 379 1.56e-16 82.8
MS.gene50751.t1 AT3G51670 26.271 236 146 8 96 323 84 299 3.56e-13 72.0
MS.gene50751.t1 AT4G09160 25.221 226 146 7 96 310 316 529 9.50e-13 71.6
MS.gene50751.t1 AT4G09160 25.221 226 146 7 96 310 316 529 1.03e-12 71.2
MS.gene50751.t1 AT4G09160 25.221 226 146 7 96 310 341 554 1.05e-12 71.2
MS.gene50751.t1 AT1G14820 26.923 234 152 7 93 323 27 244 7.91e-12 66.2
MS.gene50751.t1 AT1G14820 27.004 237 154 7 90 323 11 231 9.61e-12 65.9
MS.gene50751.t1 AT1G14820 27.004 237 154 7 90 323 11 231 9.61e-12 65.9

Find 128 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTATTGCTGCCTTCTTTA+AGG 0.090734 4.3:+34373477 None:intergenic
GAAACTACCTTCAAGATTTC+TGG 0.128425 4.3:+34371912 None:intergenic
CAGAATTATGGCCATGGTTT+TGG 0.149183 4.3:-34371194 MS.gene50751:CDS
ACTATTGATCAACCTGTTAA+AGG 0.167552 4.3:-34373843 MS.gene50751:intron
TCAATGCTGGTTCGGGATTT+AGG 0.174214 4.3:-34372396 MS.gene50751:CDS
TTGAGCAATTCCTCCATTTC+AGG 0.178864 4.3:+34370968 None:intergenic
AGGTCTTGAGATTGAATATT+TGG 0.201747 4.3:-34373527 MS.gene50751:CDS
CTCCAATGGAGGAGGGAATT+TGG 0.273143 4.3:-34373117 MS.gene50751:CDS
CAGTGAACTTCCAGAGTTCT+TGG 0.309109 4.3:-34372000 MS.gene50751:CDS
TTGAGGGTAATAGTTCTTCT+AGG 0.323926 4.3:+34373329 None:intergenic
ACAGGTTGATCAATAGTAAT+AGG 0.325672 4.3:+34373849 None:intergenic
TTCATCATCAATGCTGGTTC+GGG 0.333695 4.3:-34372403 MS.gene50751:CDS
ACCTTCCATGCCACCTGAAA+TGG 0.343417 4.3:-34370981 MS.gene50751:CDS
TAGATTTACTAACCTTTAAC+AGG 0.345398 4.3:+34373831 None:intergenic
GTGCCTTCGCCCCTTCTTGA+AGG 0.373381 4.3:+34373432 None:intergenic
GGTATATATTGATGGCAATA+TGG 0.374670 4.3:-34371098 MS.gene50751:CDS
TGAATGACAAACAAGTATCT+TGG 0.383285 4.3:-34371445 MS.gene50751:CDS
AGAAGGGGCGAAGGCACAAC+AGG 0.395434 4.3:-34373426 MS.gene50751:CDS
GGTAACAATGTATGCAAGAA+TGG 0.412286 4.3:-34371238 MS.gene50751:CDS
CCCAACCTTTCTATGTAAAC+AGG 0.413197 4.3:+34372884 None:intergenic
AGTGAACTTCCAGAGTTCTT+GGG 0.420010 4.3:-34371999 MS.gene50751:CDS
TGATCAAAGCACAACTATCT+TGG 0.420976 4.3:-34372732 MS.gene50751:CDS
GTTCATCATCAATGCTGGTT+CGG 0.428392 4.3:-34372404 MS.gene50751:CDS
CAACTCCATGTGTTCCTTGA+GGG 0.429365 4.3:+34372921 None:intergenic
ACTGATGCAAGTAACGACTA+TGG 0.432973 4.3:-34372843 MS.gene50751:CDS
AATCGATACAGAAGAGTTGC+AGG 0.433152 4.3:-34373380 MS.gene50751:CDS
GTTCAAACATTCCTTCAAGA+AGG 0.436645 4.3:-34373443 MS.gene50751:CDS
TTACATAGAAAGGTTGGGTT+TGG 0.438060 4.3:-34372879 MS.gene50751:CDS
GGAGACCTGTTTACATAGAA+AGG 0.445739 4.3:-34372889 MS.gene50751:CDS
ATCAGTACAAAATGATCAAA+AGG 0.448986 4.3:-34371417 MS.gene50751:CDS
TCGAGGTGGTCTTGGGATCA+AGG 0.450087 4.3:+34372347 None:intergenic
GGTAACTCGGTATATATTGA+TGG 0.451076 4.3:-34371106 MS.gene50751:CDS
TCCATTTCAGGTGGCATGGA+AGG 0.453045 4.3:+34370980 None:intergenic
TGATTTCAAGCAACTTGCTC+TGG 0.456669 4.3:+34372188 None:intergenic
CGTTGATGTGCAGATTGCTT+TGG 0.457244 4.3:-34371259 MS.gene50751:intron
GAATGACAAACAAGTATCTT+GGG 0.458886 4.3:-34371444 MS.gene50751:CDS
ACCTGTTTACATAGAAAGGT+TGG 0.459238 4.3:-34372885 MS.gene50751:CDS
CCTGTTTACATAGAAAGGTT+GGG 0.459264 4.3:-34372884 MS.gene50751:CDS
CAAGGGCTTCAGAAGATTGA+TGG 0.460929 4.3:-34372539 MS.gene50751:CDS
GATGATGAACATACGGTTCA+AGG 0.463960 4.3:+34372417 None:intergenic
GTACATGGTACTTAAGATAT+CGG 0.465167 4.3:+34372819 None:intergenic
CACCAAATTCCCTCCTCCAT+TGG 0.472756 4.3:+34373115 None:intergenic
CATCAATCTTCTGAAGCCCT+TGG 0.481135 4.3:+34372540 None:intergenic
TTCAAACATTCCTTCAAGAA+GGG 0.481510 4.3:-34373442 MS.gene50751:CDS
TAATTGATTGATCAGGTCCT+AGG 0.483244 4.3:-34372220 MS.gene50751:intron
AGGAACACATGGAGTTGATA+AGG 0.483607 4.3:-34372915 MS.gene50751:CDS
TTTGCTGCAATTGAACAAGC+AGG 0.492349 4.3:+34372764 None:intergenic
ATGACTGCAATTGCATTGTA+TGG 0.496484 4.3:-34371127 MS.gene50751:CDS
AACCATGGCCATAATTCTGC+CGG 0.506924 4.3:+34371198 None:intergenic
TTCCGGCAGAATTATGGCCA+TGG 0.507474 4.3:-34371200 MS.gene50751:CDS
AAGATTTCTGGATCATTCCA+TGG 0.509508 4.3:+34371924 None:intergenic
TGCTCTGGTATTTGTTACCT+AGG 0.510194 4.3:+34372203 None:intergenic
TGCTTGAAATCATCGATGCC+AGG 0.510523 4.3:-34372177 MS.gene50751:intron
TTCCTCCATTTCAGGTGGCA+TGG 0.511464 4.3:+34370976 None:intergenic
GTTTGTGATTGCAGTTCCAC+TGG 0.517448 4.3:-34370639 MS.gene50751:intron
GAAAACTTTATCCGAAGTTG+AGG 0.528688 4.3:-34371773 MS.gene50751:CDS
GATGAAAAGAGAACAAGGCT+TGG 0.532278 4.3:-34373502 MS.gene50751:CDS
ATTCACCTTCTCTTCTAGAT+CGG 0.535193 4.3:+34371018 None:intergenic
TCTTGAGATTGAATATTTGG+AGG 0.535896 4.3:-34373524 MS.gene50751:CDS
AGAGTTGGCTACAGCCAAGA+AGG 0.536487 4.3:-34370912 MS.gene50751:intron
TGCATATCAGTTCGACACCT+TGG 0.542514 4.3:-34371474 MS.gene50751:CDS
CAGATATGCTCCAATGGAGG+AGG 0.542666 4.3:-34373125 MS.gene50751:CDS
ATCATGTGTGACACTATGTC+TGG 0.544111 4.3:-34373873 MS.gene50751:CDS
ATGATCATCATATGATGCTA+AGG 0.545044 4.3:-34373300 MS.gene50751:intron
ATGAATTAGATGGTTCAAAA+CGG 0.546710 4.3:-34371814 MS.gene50751:intron
CGTATGTTCATCATCAATGC+TGG 0.547791 4.3:-34372409 MS.gene50751:CDS
GTGAACTTCCAGAGTTCTTG+GGG 0.551248 4.3:-34371998 MS.gene50751:CDS
ACAAGCAGGCATCTTAACAT+TGG 0.553424 4.3:+34372778 None:intergenic
ACACATGGAGTTGATAAGGA+TGG 0.553554 4.3:-34372911 MS.gene50751:CDS
TGGTTGCATGCTTTCTGACA+AGG 0.554475 4.3:-34371949 MS.gene50751:CDS
TTTGTCATTCATTGAGACCA+AGG 0.554617 4.3:+34371457 None:intergenic
TCTAAACCGTGTCGATACAC+TGG 0.555753 4.3:-34370939 MS.gene50751:CDS
ATTTGGTGCCGATACTATCA+TGG 0.556047 4.3:-34373100 MS.gene50751:CDS
GAATGATCCAGAAATCTTGA+AGG 0.557107 4.3:-34371919 MS.gene50751:intron
CATTTCCGTTGATGATCACA+TGG 0.558838 4.3:-34371059 MS.gene50751:CDS
CACATGGAGTTGATAAGGAT+GGG 0.560270 4.3:-34372910 MS.gene50751:CDS
TCTTCTCAAACTCTCGTACA+TGG 0.560760 4.3:+34372804 None:intergenic
CGATACACTGGAGCAAGAGT+TGG 0.564279 4.3:-34370927 MS.gene50751:CDS
TCAACTCCATGTGTTCCTTG+AGG 0.566578 4.3:+34372920 None:intergenic
TGCTTTCTGACAAGGGACCA+TGG 0.567626 4.3:-34371941 MS.gene50751:CDS
TACTACCCTCAAGGAACACA+TGG 0.568354 4.3:-34372926 MS.gene50751:CDS
AATGTAGATTCTTGAGAGCA+AGG 0.568501 4.3:-34373182 MS.gene50751:intron
TTCCATGCCACCTGAAATGG+AGG 0.574584 4.3:-34370978 MS.gene50751:CDS
CAGCATTGATGATGAACATA+CGG 0.574837 4.3:+34372410 None:intergenic
TGATCATCAACGGAAATGAC+AGG 0.575643 4.3:+34371064 None:intergenic
TCAAACATTCCTTCAAGAAG+GGG 0.577257 4.3:-34373441 MS.gene50751:CDS
GTTCTAAAATACTACCCTCA+AGG 0.581819 4.3:-34372935 MS.gene50751:CDS
GAAGCTCCACTTGCCTAGCA+AGG 0.584248 4.3:+34370792 None:intergenic
GGTTGCATGCTTTCTGACAA+GGG 0.584666 4.3:-34371948 MS.gene50751:CDS
CATGACCGATCTAGAAGAGA+AGG 0.585875 4.3:-34371023 MS.gene50751:CDS
AGCAATTCCTCCATTTCAGG+TGG 0.586975 4.3:+34370971 None:intergenic
AAATACCTCCATGATAGTAT+CGG 0.587094 4.3:+34373092 None:intergenic
AGACGAAGGAGTGTGTCAAA+AGG 0.590474 4.3:-34371734 MS.gene50751:intron
CACTTGATGATGCCCTTGCT+AGG 0.595315 4.3:-34370805 MS.gene50751:CDS
AATTGCATTGTATGGTAACT+CGG 0.595923 4.3:-34371119 MS.gene50751:CDS
GGTGGTCTTGGGATCAAGGA+AGG 0.597891 4.3:+34372351 None:intergenic
GCTGCAAGAGATCTCATCCA+AGG 0.597936 4.3:-34372557 MS.gene50751:CDS
AAAACAATCGTCGAAGACGA+AGG 0.598967 4.3:-34371748 MS.gene50751:CDS
AAGGCTTGGATCCTTAAAGA+AGG 0.599891 4.3:-34373488 MS.gene50751:CDS
TAGAGCCATGTGATCATCAA+CGG 0.599949 4.3:+34371054 None:intergenic
AACTTACTTCTGCTATAGCA+TGG 0.605936 4.3:+34371582 None:intergenic
AAGCTCCACTTGCCTAGCAA+GGG 0.609549 4.3:+34370793 None:intergenic
ACAACTATCTTGGATGTCCA+AGG 0.610723 4.3:-34372722 MS.gene50751:CDS
TGACGTTCATCCGCATGACA+CGG 0.612693 4.3:-34371165 MS.gene50751:CDS
CTGCAAGAGATCTCATCCAA+GGG 0.614690 4.3:-34372556 MS.gene50751:CDS
ATTCCTTCAAGAAGGGGCGA+AGG 0.615207 4.3:-34373435 MS.gene50751:CDS
TGTGGGCAGATATGCTCCAA+TGG 0.615883 4.3:-34373131 MS.gene50751:CDS
CAACAAAGAATACGTTCTCG+AGG 0.621800 4.3:-34371612 MS.gene50751:CDS
TGGAGGATGAAAAGAGAACA+AGG 0.626518 4.3:-34373507 MS.gene50751:CDS
CCTTAGTAATTAATTACCAG+TGG 0.627692 4.3:+34370623 None:intergenic
ACGGTGAAGCAAAATGCAGA+AGG 0.628053 4.3:-34371795 MS.gene50751:CDS
TTGGTATACTCCGTGTCATG+CGG 0.646880 4.3:+34371155 None:intergenic
CTTGCTCCAGTGTATCGACA+CGG 0.648033 4.3:+34370933 None:intergenic
TATCTTGGATGTCCAAGGAG+TGG 0.654369 4.3:-34372717 MS.gene50751:intron
ACTTGTACTTGTGCAGACAA+AGG 0.654424 4.3:-34371972 MS.gene50751:CDS
CGTCATGATTCCCAAAACCA+TGG 0.657898 4.3:+34371183 None:intergenic
AGATATGCTCCAATGGAGGA+GGG 0.660017 4.3:-34373124 MS.gene50751:CDS
CAAAGAATACGTTCTCGAGG+AGG 0.660775 4.3:-34371609 MS.gene50751:CDS
TGACAAACAAGTATCTTGGG+AGG 0.664438 4.3:-34371441 MS.gene50751:CDS
TGATGCCCTTGCTAGGCAAG+TGG 0.666904 4.3:-34370798 MS.gene50751:CDS
GAACTTCCAGAGTTCTTGGG+GGG 0.675384 4.3:-34371996 MS.gene50751:CDS
GGGCAGATATGCTCCAATGG+AGG 0.678590 4.3:-34373128 MS.gene50751:CDS
CAGGGTTATATCTGTCATCG+AGG 0.687126 4.3:-34373407 MS.gene50751:CDS
TGAACTTCCAGAGTTCTTGG+GGG 0.688265 4.3:-34371997 MS.gene50751:CDS
TGTACTTGTGCAGACAAAGG+TGG 0.715199 4.3:-34371969 MS.gene50751:CDS
GAAGGGGCGAAGGCACAACA+GGG 0.715484 4.3:-34373425 MS.gene50751:CDS
TGATGGTGACAACTATCCTG+AGG 0.717578 4.3:-34372522 MS.gene50751:intron
TGGTGCCGATACTATCATGG+AGG 0.741681 4.3:-34373097 MS.gene50751:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTATGTTCAAATTTA+AGG + chr4.3:34370814-34370833 None:intergenic 10.0%
!!! ATTTATTTGTTCCTTTTTAT+TGG - chr4.3:34373671-34373690 MS.gene50751:intron 15.0%
!!! TTTTAATTTATGCAAAAAAC+AGG - chr4.3:34370959-34370978 MS.gene50751:CDS 15.0%
!! AAAGAAAAAGAAGAAAAAGT+TGG - chr4.3:34373753-34373772 MS.gene50751:intron 20.0%
!! ACACAAAAAAACAATGTAAA+TGG - chr4.3:34370880-34370899 MS.gene50751:intron 20.0%
!! ATATTACTAATTGATTGATC+AGG - chr4.3:34372279-34372298 MS.gene50751:intron 20.0%
!! ATATTTATAGAAACAATGCA+TGG + chr4.3:34373815-34373834 None:intergenic 20.0%
!! GAATATCATAAGAAAAAGAA+AGG + chr4.3:34372858-34372877 None:intergenic 20.0%
!! GTATTAGAATATGAAATTTC+AGG - chr4.3:34370788-34370807 MS.gene50751:CDS 20.0%
!! TGACTAACATAAATTTCTTT+CGG - chr4.3:34371511-34371530 MS.gene50751:intron 20.0%
!! TGATCAATCAATTAGTAATA+TGG + chr4.3:34372280-34372299 None:intergenic 20.0%
!!! TCTTTTTCTTATGATATTCA+GGG - chr4.3:34372859-34372878 MS.gene50751:CDS 20.0%
!!! TTCTTTTTCTTATGATATTC+AGG - chr4.3:34372858-34372877 MS.gene50751:CDS 20.0%
! ATCAGTACAAAATGATCAAA+AGG - chr4.3:34373089-34373108 MS.gene50751:intron 25.0%
! ATGAATTAGATGGTTCAAAA+CGG - chr4.3:34372692-34372711 MS.gene50751:intron 25.0%
! TAAAGAAGAAAATATAGACC+TGG + chr4.3:34372350-34372369 None:intergenic 25.0%
! TACAAAATAAAAAGCTTACG+TGG + chr4.3:34372189-34372208 None:intergenic 25.0%
! TAGATTTACTAACCTTTAAC+AGG + chr4.3:34370678-34370697 None:intergenic 25.0%
! TATTCGTATTATGTGACATA+TGG + chr4.3:34372443-34372462 None:intergenic 25.0%
! TCTTGAAACTATAAAGAAGT+AGG + chr4.3:34372808-34372827 None:intergenic 25.0%
! TGAAAAAACAAAGCAAATGT+GGG - chr4.3:34371358-34371377 MS.gene50751:intron 25.0%
! TTGAAAAAACAAAGCAAATG+TGG - chr4.3:34371357-34371376 MS.gene50751:intron 25.0%
! TTTAAGAAATTGACCATGAA+AGG + chr4.3:34370727-34370746 None:intergenic 25.0%
!! AAAAAGAAGAAAAAGTTGGT+AGG - chr4.3:34373757-34373776 MS.gene50751:intron 25.0%
!! GGATTTAGAAATCAATTTTC+CGG - chr4.3:34373289-34373308 MS.gene50751:intron 25.0%
!! TCTTTACTGTGTTGATAAAA+TGG + chr4.3:34373194-34373213 None:intergenic 25.0%
!!! AATTTCTTTCGGTTTTGTTA+AGG - chr4.3:34371522-34371541 MS.gene50751:intron 25.0%
!!! ATTTAACTCCTTGTGTTTTT+TGG - chr4.3:34372478-34372497 MS.gene50751:intron 25.0%
!!! TTTAACTCCTTGTGTTTTTT+GGG - chr4.3:34372479-34372498 MS.gene50751:intron 25.0%
!!! TTTTTTCCTGATGAATTAGA+TGG - chr4.3:34372682-34372701 MS.gene50751:intron 25.0%
!!! TTTTTTTATGTATGAAGCTG+AGG - chr4.3:34370829-34370848 MS.gene50751:intron 25.0%
AAATACCTCCATGATAGTAT+CGG + chr4.3:34371417-34371436 None:intergenic 30.0%
AAATCTTGAAGGTAGTTTCA+CGG - chr4.3:34372598-34372617 MS.gene50751:intron 30.0%
AACTGTCTAATCAAAGCATA+AGG + chr4.3:34371271-34371290 None:intergenic 30.0%
AATTGCATTGTATGGTAACT+CGG - chr4.3:34373387-34373406 MS.gene50751:CDS 30.0%
ACTATTGATCAACCTGTTAA+AGG - chr4.3:34370663-34370682 MS.gene50751:intron 30.0%
AGGTCTTGAGATTGAATATT+TGG - chr4.3:34370979-34370998 MS.gene50751:CDS 30.0%
ATGATCATCATATGATGCTA+AGG - chr4.3:34371206-34371225 MS.gene50751:CDS 30.0%
ATTTACTGTATGAGTCTCAA+GGG + chr4.3:34371235-34371254 None:intergenic 30.0%
GAATGACAAACAAGTATCTT+GGG - chr4.3:34373062-34373081 MS.gene50751:intron 30.0%
GTACATGGTACTTAAGATAT+CGG + chr4.3:34371690-34371709 None:intergenic 30.0%
TAAGTACTTACTGTATAGCA+TGG - chr4.3:34371897-34371916 MS.gene50751:intron 30.0%
TATTTACTGTATGAGTCTCA+AGG + chr4.3:34371236-34371255 None:intergenic 30.0%
TCTTGAGATTGAATATTTGG+AGG - chr4.3:34370982-34371001 MS.gene50751:CDS 30.0%
TGAAAAGAAAAGTGGAACTA+AGG + chr4.3:34372063-34372082 None:intergenic 30.0%
TGAATGACAAACAAGTATCT+TGG - chr4.3:34373061-34373080 MS.gene50751:intron 30.0%
TTCAAACATTCCTTCAAGAA+GGG - chr4.3:34371064-34371083 MS.gene50751:CDS 30.0%
TTTGAACCATCTAATTCATC+AGG + chr4.3:34372691-34372710 None:intergenic 30.0%
! ATAGTTTCAAGAGCATAGTT+TGG - chr4.3:34372816-34372835 MS.gene50751:CDS 30.0%
! ATGATGATCATCATGTTTTG+AGG + chr4.3:34371197-34371216 None:intergenic 30.0%
! GGTATATATTGATGGCAATA+TGG - chr4.3:34373408-34373427 MS.gene50751:CDS 30.0%
! TGTTAAGGATTTTGAATTCG+AGG - chr4.3:34371537-34371556 MS.gene50751:intron 30.0%
! TGTTCCTTTTTATTGGCATT+AGG - chr4.3:34373678-34373697 MS.gene50751:intron 30.0%
!! ACAGGTTGATCAATAGTAAT+AGG + chr4.3:34370660-34370679 None:intergenic 30.0%
!! TGATGATCATCATGTTTTGA+GGG + chr4.3:34371196-34371215 None:intergenic 30.0%
AAATTGACCATGAAAGGCAA+AGG + chr4.3:34370721-34370740 None:intergenic 35.0%
AACTTACTTCTGCTATAGCA+TGG + chr4.3:34372927-34372946 None:intergenic 35.0%
AATGTAGATTCTTGAGAGCA+AGG - chr4.3:34371324-34371343 MS.gene50751:intron 35.0%
ACCTGTTTACATAGAAAGGT+TGG - chr4.3:34371621-34371640 MS.gene50751:CDS 35.0%
ACTTATTGCTGCCTTCTTTA+AGG + chr4.3:34371032-34371051 None:intergenic 35.0%
AGTGCCTAATGCCAATAAAA+AGG + chr4.3:34373685-34373704 None:intergenic 35.0%
ATGACTGCAATTGCATTGTA+TGG - chr4.3:34373379-34373398 MS.gene50751:CDS 35.0%
ATTCACCTTCTCTTCTAGAT+CGG + chr4.3:34373491-34373510 None:intergenic 35.0%
CAAGAAAAACAGTACCTTCT+TGG + chr4.3:34373611-34373630 None:intergenic 35.0%
CCTGTTTACATAGAAAGGTT+GGG - chr4.3:34371622-34371641 MS.gene50751:CDS 35.0%
CGTATTATGTGACATATGGA+TGG + chr4.3:34372439-34372458 None:intergenic 35.0%
CTGTTAACCCAAAAAACACA+AGG + chr4.3:34372489-34372508 None:intergenic 35.0%
GAAAACTTTATCCGAAGTTG+AGG - chr4.3:34372733-34372752 MS.gene50751:CDS 35.0%
GAAACTACCTTCAAGATTTC+TGG + chr4.3:34372597-34372616 None:intergenic 35.0%
GAATGATCCAGAAATCTTGA+AGG - chr4.3:34372587-34372606 MS.gene50751:intron 35.0%
GGACTGTATGAAAAGAAAAG+TGG + chr4.3:34372071-34372090 None:intergenic 35.0%
GGTAACAATGTATGCAAGAA+TGG - chr4.3:34373268-34373287 MS.gene50751:intron 35.0%
GGTAACTCGGTATATATTGA+TGG - chr4.3:34373400-34373419 MS.gene50751:CDS 35.0%
GTAGGTAAAAATAGTACCTC+AGG + chr4.3:34372003-34372022 None:intergenic 35.0%
GTTCAAACATTCCTTCAAGA+AGG - chr4.3:34371063-34371082 MS.gene50751:CDS 35.0%
TAATTGATTGATCAGGTCCT+AGG - chr4.3:34372286-34372305 MS.gene50751:intron 35.0%
TCAAACATTCCTTCAAGAAG+GGG - chr4.3:34371065-34371084 MS.gene50751:CDS 35.0%
TGATCAAAGCACAACTATCT+TGG - chr4.3:34371774-34371793 MS.gene50751:CDS 35.0%
TTACATAGAAAGGTTGGGTT+TGG - chr4.3:34371627-34371646 MS.gene50751:CDS 35.0%
TTGAGGGTAATAGTTCTTCT+AGG + chr4.3:34371180-34371199 None:intergenic 35.0%
TTTGTCATTCATTGAGACCA+AGG + chr4.3:34373052-34373071 None:intergenic 35.0%
! AAGATTTCTGGATCATTCCA+TGG + chr4.3:34372585-34372604 None:intergenic 35.0%
! AGAATTATGGCCATGGTTTT+GGG - chr4.3:34373313-34373332 MS.gene50751:CDS 35.0%
! GATTGTTTTCTCCTCAACTT+CGG + chr4.3:34372747-34372766 None:intergenic 35.0%
! TCAATTTTCCGGCAGAATTA+TGG - chr4.3:34373300-34373319 MS.gene50751:intron 35.0%
! TTTTACCTACTCGAGCATAT+TGG - chr4.3:34372013-34372032 MS.gene50751:intron 35.0%
! TTTTGATACATACCACTCCT+TGG + chr4.3:34371804-34371823 None:intergenic 35.0%
!! CAGCATTGATGATGAACATA+CGG + chr4.3:34372099-34372118 None:intergenic 35.0%
!! GTTCTAAAATACTACCCTCA+AGG - chr4.3:34371571-34371590 MS.gene50751:intron 35.0%
!!! GCAATTGCAGTCATTTTTGT+TGG + chr4.3:34373373-34373392 None:intergenic 35.0%
AAAACAATCGTCGAAGACGA+AGG - chr4.3:34372758-34372777 MS.gene50751:CDS 40.0%
AAGGCTTGGATCCTTAAAGA+AGG - chr4.3:34371018-34371037 MS.gene50751:CDS 40.0%
AATCGATACAGAAGAGTTGC+AGG - chr4.3:34371126-34371145 MS.gene50751:CDS 40.0%
ACAACTATCTTGGATGTCCA+AGG - chr4.3:34371784-34371803 MS.gene50751:CDS 40.0%
ACAAGCAGGCATCTTAACAT+TGG + chr4.3:34371731-34371750 None:intergenic 40.0%
ACTGATGCAAGTAACGACTA+TGG - chr4.3:34371663-34371682 MS.gene50751:intron 40.0%
ACTGTATAGCATGGTTGATC+AGG - chr4.3:34371906-34371925 MS.gene50751:intron 40.0%
ACTTGTACTTGTGCAGACAA+AGG - chr4.3:34372534-34372553 MS.gene50751:CDS 40.0%
AGTGAACTTCCAGAGTTCTT+GGG - chr4.3:34372507-34372526 MS.gene50751:intron 40.0%
CAAAGCAATCTGCACATCAA+CGG + chr4.3:34373249-34373268 None:intergenic 40.0%
CAACAAAGAATACGTTCTCG+AGG - chr4.3:34372894-34372913 MS.gene50751:CDS 40.0%
CATTACACCTTTGCCTTTCA+TGG - chr4.3:34370711-34370730 MS.gene50751:intron 40.0%
CCCAACCTTTCTATGTAAAC+AGG + chr4.3:34371625-34371644 None:intergenic 40.0%
CCTGCAAAATTGAAAGTGAG+TGG + chr4.3:34372996-34373015 None:intergenic 40.0%
CGTATGTTCATCATCAATGC+TGG - chr4.3:34372097-34372116 MS.gene50751:intron 40.0%
CTGTATAGCATGGTTGATCA+GGG - chr4.3:34371907-34371926 MS.gene50751:intron 40.0%
GATGAAAAGAGAACAAGGCT+TGG - chr4.3:34371004-34371023 MS.gene50751:CDS 40.0%
GATGATGAACATACGGTTCA+AGG + chr4.3:34372092-34372111 None:intergenic 40.0%
GGAGACCTGTTTACATAGAA+AGG - chr4.3:34371617-34371636 MS.gene50751:CDS 40.0%
TAGAGCCATGTGATCATCAA+CGG + chr4.3:34373455-34373474 None:intergenic 40.0%
TCTTCTCAAACTCTCGTACA+TGG + chr4.3:34371705-34371724 None:intergenic 40.0%
TGACAAACAAGTATCTTGGG+AGG - chr4.3:34373065-34373084 MS.gene50751:intron 40.0%
TGATCATCAACGGAAATGAC+AGG + chr4.3:34373445-34373464 None:intergenic 40.0%
TGGAGGATGAAAAGAGAACA+AGG - chr4.3:34370999-34371018 MS.gene50751:CDS 40.0%
TTGAACCAATATGCTCGAGT+AGG + chr4.3:34372021-34372040 None:intergenic 40.0%
TTGAGCAATTCCTCCATTTC+AGG + chr4.3:34373541-34373560 None:intergenic 40.0%
TTTGCTGCAATTGAACAAGC+AGG + chr4.3:34371745-34371764 None:intergenic 40.0%
! AAAGCTTACGTGGATTTTCG+AGG + chr4.3:34372179-34372198 None:intergenic 40.0%
! CAGAATTATGGCCATGGTTT+TGG - chr4.3:34373312-34373331 MS.gene50751:CDS 40.0%
! CCACTCACTTTCAATTTTGC+AGG - chr4.3:34372993-34373012 MS.gene50751:intron 40.0%
! GTTCATCATCAATGCTGGTT+CGG - chr4.3:34372102-34372121 MS.gene50751:intron 40.0%
! TGATTTCAAGCAACTTGCTC+TGG + chr4.3:34372321-34372340 None:intergenic 40.0%
! TGCTCTGGTATTTGTTACCT+AGG + chr4.3:34372306-34372325 None:intergenic 40.0%
!! ACACATGGAGTTGATAAGGA+TGG - chr4.3:34371595-34371614 MS.gene50751:CDS 40.0%
!! AGGAACACATGGAGTTGATA+AGG - chr4.3:34371591-34371610 MS.gene50751:CDS 40.0%
!! ATTTGGTGCCGATACTATCA+TGG - chr4.3:34371406-34371425 MS.gene50751:CDS 40.0%
!! CACATGGAGTTGATAAGGAT+GGG - chr4.3:34371596-34371615 MS.gene50751:CDS 40.0%
!! CATTTCCGTTGATGATCACA+TGG - chr4.3:34373447-34373466 MS.gene50751:CDS 40.0%
!! GTTCGGGATTTAGGATTTTG+TGG - chr4.3:34372119-34372138 MS.gene50751:intron 40.0%
!! TTCATCATCAATGCTGGTTC+GGG - chr4.3:34372103-34372122 MS.gene50751:intron 40.0%
AACCATGGCCATAATTCTGC+CGG + chr4.3:34373311-34373330 None:intergenic 45.0%
ACGGTGAAGCAAAATGCAGA+AGG - chr4.3:34372711-34372730 MS.gene50751:intron 45.0%
AGATATGCTCCAATGGAGGA+GGG - chr4.3:34371382-34371401 MS.gene50751:intron 45.0%
AGCAATTCCTCCATTTCAGG+TGG + chr4.3:34373538-34373557 None:intergenic 45.0%
CAAAGAATACGTTCTCGAGG+AGG - chr4.3:34372897-34372916 MS.gene50751:CDS 45.0%
CAACTCCATGTGTTCCTTGA+GGG + chr4.3:34371588-34371607 None:intergenic 45.0%
CAAGGGCTTCAGAAGATTGA+TGG - chr4.3:34371967-34371986 MS.gene50751:CDS 45.0%
CAGGGTTATATCTGTCATCG+AGG - chr4.3:34371099-34371118 MS.gene50751:CDS 45.0%
CAGTGAACTTCCAGAGTTCT+TGG - chr4.3:34372506-34372525 MS.gene50751:intron 45.0%
CATGACCGATCTAGAAGAGA+AGG - chr4.3:34373483-34373502 MS.gene50751:CDS 45.0%
CGTCATGATTCCCAAAACCA+TGG + chr4.3:34373326-34373345 None:intergenic 45.0%
CGTTGATGTGCAGATTGCTT+TGG - chr4.3:34373247-34373266 MS.gene50751:intron 45.0%
CTGCAAGAGATCTCATCCAA+GGG - chr4.3:34371950-34371969 MS.gene50751:CDS 45.0%
GGTTGCATGCTTTCTGACAA+GGG - chr4.3:34372558-34372577 MS.gene50751:CDS 45.0%
GTGAACTTCCAGAGTTCTTG+GGG - chr4.3:34372508-34372527 MS.gene50751:intron 45.0%
GTTTGTGATTGCAGTTCCAC+TGG - chr4.3:34373867-34373886 MS.gene50751:CDS 45.0%
TACTACCCTCAAGGAACACA+TGG - chr4.3:34371580-34371599 MS.gene50751:intron 45.0%
TATCTTGGATGTCCAAGGAG+TGG - chr4.3:34371789-34371808 MS.gene50751:CDS 45.0%
TCAACTCCATGTGTTCCTTG+AGG + chr4.3:34371589-34371608 None:intergenic 45.0%
TGAACTTCCAGAGTTCTTGG+GGG - chr4.3:34372509-34372528 MS.gene50751:intron 45.0%
TGCATATCAGTTCGACACCT+TGG - chr4.3:34373032-34373051 MS.gene50751:intron 45.0%
TGCTTGAAATCATCGATGCC+AGG - chr4.3:34372329-34372348 MS.gene50751:intron 45.0%
TGGTTGCATGCTTTCTGACA+AGG - chr4.3:34372557-34372576 MS.gene50751:CDS 45.0%
TGTACTTGTGCAGACAAAGG+TGG - chr4.3:34372537-34372556 MS.gene50751:CDS 45.0%
TTGGTATACTCCGTGTCATG+CGG + chr4.3:34373354-34373373 None:intergenic 45.0%
! AGACGAAGGAGTGTGTCAAA+AGG - chr4.3:34372772-34372791 MS.gene50751:CDS 45.0%
! TCTAAACCGTGTCGATACAC+TGG - chr4.3:34373567-34373586 MS.gene50751:intron 45.0%
! TGATGGTGACAACTATCCTG+AGG - chr4.3:34371984-34372003 MS.gene50751:CDS 45.0%
!! CATCAATCTTCTGAAGCCCT+TGG + chr4.3:34371969-34371988 None:intergenic 45.0%
!! TCAATGCTGGTTCGGGATTT+AGG - chr4.3:34372110-34372129 MS.gene50751:intron 45.0%
!! TGGATTTTCGAGGTGGTCTT+GGG + chr4.3:34372169-34372188 None:intergenic 45.0%
AAGCTCCACTTGCCTAGCAA+GGG + chr4.3:34373716-34373735 None:intergenic 50.0%
ACCTTCCATGCCACCTGAAA+TGG - chr4.3:34373525-34373544 MS.gene50751:CDS 50.0%
AGAGTTGGCTACAGCCAAGA+AGG - chr4.3:34373594-34373613 MS.gene50751:intron 50.0%
ATTCCTTCAAGAAGGGGCGA+AGG - chr4.3:34371071-34371090 MS.gene50751:CDS 50.0%
CACCAAATTCCCTCCTCCAT+TGG + chr4.3:34371394-34371413 None:intergenic 50.0%
CAGATATGCTCCAATGGAGG+AGG - chr4.3:34371381-34371400 MS.gene50751:intron 50.0%
CGATACACTGGAGCAAGAGT+TGG - chr4.3:34373579-34373598 MS.gene50751:intron 50.0%
CTCCAATGGAGGAGGGAATT+TGG - chr4.3:34371389-34371408 MS.gene50751:intron 50.0%
GAACTTCCAGAGTTCTTGGG+GGG - chr4.3:34372510-34372529 MS.gene50751:intron 50.0%
GCTGCAAGAGATCTCATCCA+AGG - chr4.3:34371949-34371968 MS.gene50751:CDS 50.0%
TGACGTTCATCCGCATGACA+CGG - chr4.3:34373341-34373360 MS.gene50751:CDS 50.0%
TGCTTTCTGACAAGGGACCA+TGG - chr4.3:34372565-34372584 MS.gene50751:CDS 50.0%
TGTGGGCAGATATGCTCCAA+TGG - chr4.3:34371375-34371394 MS.gene50751:intron 50.0%
TTCCATGCCACCTGAAATGG+AGG - chr4.3:34373528-34373547 MS.gene50751:CDS 50.0%
TTCCGGCAGAATTATGGCCA+TGG - chr4.3:34373306-34373325 MS.gene50751:CDS 50.0%
! CTTGCTCCAGTGTATCGACA+CGG + chr4.3:34373576-34373595 None:intergenic 50.0%
! TCCATTTCAGGTGGCATGGA+AGG + chr4.3:34373529-34373548 None:intergenic 50.0%
! TTCCTCCATTTCAGGTGGCA+TGG + chr4.3:34373533-34373552 None:intergenic 50.0%
!! CACTTGATGATGCCCTTGCT+AGG - chr4.3:34373701-34373720 MS.gene50751:intron 50.0%
!! GCTTACGTGGATTTTCGAGG+TGG + chr4.3:34372176-34372195 None:intergenic 50.0%
!! GTGGATTTTCGAGGTGGTCT+TGG + chr4.3:34372170-34372189 None:intergenic 50.0%
!! TGGTGCCGATACTATCATGG+AGG - chr4.3:34371409-34371428 MS.gene50751:CDS 50.0%
CAAGTACCCCCCAAGAACTC+TGG + chr4.3:34372519-34372538 None:intergenic 55.0%
GAAGCTCCACTTGCCTAGCA+AGG + chr4.3:34373717-34373736 None:intergenic 55.0%
GGGCAGATATGCTCCAATGG+AGG - chr4.3:34371378-34371397 MS.gene50751:intron 55.0%
TGATGCCCTTGCTAGGCAAG+TGG - chr4.3:34373708-34373727 MS.gene50751:intron 55.0%
! GGTGGTCTTGGGATCAAGGA+AGG + chr4.3:34372158-34372177 None:intergenic 55.0%
! TCGAGGTGGTCTTGGGATCA+AGG + chr4.3:34372162-34372181 None:intergenic 55.0%
GTGCCTTCGCCCCTTCTTGA+AGG + chr4.3:34371077-34371096 None:intergenic 60.0%
! AGAAGGGGCGAAGGCACAAC+AGG - chr4.3:34371080-34371099 MS.gene50751:CDS 60.0%
! GAAGGGGCGAAGGCACAACA+GGG - chr4.3:34371081-34371100 MS.gene50751:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 34370636 34373892 34370636 ID=MS.gene50751
chr4.3 mRNA 34370636 34373892 34370636 ID=MS.gene50751.t1;Parent=MS.gene50751
chr4.3 exon 34373844 34373892 34373844 ID=MS.gene50751.t1.exon1;Parent=MS.gene50751.t1
chr4.3 CDS 34373844 34373892 34373844 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34373301 34373547 34373301 ID=MS.gene50751.t1.exon2;Parent=MS.gene50751.t1
chr4.3 CDS 34373301 34373547 34373301 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34373098 34373197 34373098 ID=MS.gene50751.t1.exon3;Parent=MS.gene50751.t1
chr4.3 CDS 34373098 34373197 34373098 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34372718 34372984 34372718 ID=MS.gene50751.t1.exon4;Parent=MS.gene50751.t1
chr4.3 CDS 34372718 34372984 34372718 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34372523 34372600 34372523 ID=MS.gene50751.t1.exon5;Parent=MS.gene50751.t1
chr4.3 CDS 34372523 34372600 34372523 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34372339 34372440 34372339 ID=MS.gene50751.t1.exon6;Parent=MS.gene50751.t1
chr4.3 CDS 34372339 34372440 34372339 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34372178 34372227 34372178 ID=MS.gene50751.t1.exon7;Parent=MS.gene50751.t1
chr4.3 CDS 34372178 34372227 34372178 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34371920 34372019 34371920 ID=MS.gene50751.t1.exon8;Parent=MS.gene50751.t1
chr4.3 CDS 34371920 34372019 34371920 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34371735 34371827 34371735 ID=MS.gene50751.t1.exon9;Parent=MS.gene50751.t1
chr4.3 CDS 34371735 34371827 34371735 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34371589 34371648 34371589 ID=MS.gene50751.t1.exon10;Parent=MS.gene50751.t1
chr4.3 CDS 34371589 34371648 34371589 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34371405 34371513 34371405 ID=MS.gene50751.t1.exon11;Parent=MS.gene50751.t1
chr4.3 CDS 34371405 34371513 34371405 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34370913 34371268 34370913 ID=MS.gene50751.t1.exon12;Parent=MS.gene50751.t1
chr4.3 CDS 34370913 34371268 34370913 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34370754 34370828 34370754 ID=MS.gene50751.t1.exon13;Parent=MS.gene50751.t1
chr4.3 CDS 34370754 34370828 34370754 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
chr4.3 exon 34370636 34370647 34370636 ID=MS.gene50751.t1.exon14;Parent=MS.gene50751.t1
chr4.3 CDS 34370636 34370647 34370636 ID=cds.MS.gene50751.t1;Parent=MS.gene50751.t1
Gene Sequence

>MS.gene50751

ATGTGTGACACTATGTCTGGACCTATTACTATTGATCAACCTGTTAAAGGTTAGTAAATCTATATTATTGCAATTCATTACACCTTTGCCTTTCATGGTCAATTTCTTAAACTTTGTTATTATTATATATATATATATATATAATCACGTTTGTATTAGAATATGAAATTTCAGGCCTTAAATTTGAACATAATTTTTTTATGTATGAAGCTGAGGAAAATGAAAAAATAGAAACTTTTTTTTGACACAAAAAAACAATGTAAATGGAATGCATCTTGTTCAAACTTTTGTTAGTAAGTCTTATTTTATTTTATTTTTTATTTTTTTAATTTATGCAAAAAACAGGTCTTGAGATTGAATATTTGGAGGATGAAAAGAGAACAAGGCTTGGATCCTTAAAGAAGGCAGCAATAAGTGCATCTTCTAAGTTCAAACATTCCTTCAAGAAGGGGCGAAGGCACAACAGGGTTATATCTGTCATCGAGGATGAAATCGATACAGAAGAGTTGCAGGCAATTGACGCTTTTCGTCAAACACTTATCCTAGAAGAACTATTACCCTCAAAACATGATGATCATCATATGATGCTAAGGTAGCCCTTGAGACTCATACAGTAAATAGCTGCAGCATCACCTTATGCTTTGATTAGACAGTTCTTAATGTGCTATTATTAATGTAATTATATGATAATGTAGATTCTTGAGAGCAAGGAAGTTTGACATTGAAAAAACAAAGCAAATGTGGGCAGATATGCTCCAATGGAGGAGGGAATTTGGTGCCGATACTATCATGGAGGTATTTTAGAGAAAAACTAAATATAAATTAAGAAAAACACTAATTGTAATTATATTGTTTTATGCAACATTTTAGTTATGTGACTAACATAAATTTCTTTCGGTTTTGTTAAGGATTTTGAATTCGAGGAGATAGACGAAGTTCTAAAATACTACCCTCAAGGAACACATGGAGTTGATAAGGATGGGAGACCTGTTTACATAGAAAGGTTGGGTTTGGTTGACTCAAACAAACTGATGCAAGTAACGACTATGGACCGATATCTTAAGTACCATGTACGAGAGTTTGAGAAGACATCCAATGTTAAGATGCCTGCTTGTTCAATTGCAGCAAAGAAGCACATTGATCAAAGCACAACTATCTTGGATGTCCAAGGAGTGGTATGTATCAAAATCATACAACACTAAATAACTTATTATACTTTGTATCATATTATAACTTGTTATATAAGTAAAGAAGTTTCAAATAAGTACTTACTGTATAGCATGGTTGATCAGGGTCTCAAAAGTATGAATAAAGCTGCAAGAGATCTCATCCAAGGGCTTCAGAAGATTGATGGTGACAACTATCCTGAGGTACTATTTTTACCTACTCGAGCATATTGGTTCAAATGTAATCTTCTGACATTACCTTAGTTCCACTTTTCTTTTCATACAGTCCTTGAACCGTATGTTCATCATCAATGCTGGTTCGGGATTTAGGATTTTGTGGAACACAGTTAAGTCCTTCCTTGATCCCAAGACCACCTCGAAAATCCACGTAAGCTTTTTATTTTGTATTTTTTTTATATACTTAACAGTTTGAAGTAATGTTGAATTTCTAATTTCAGTTAAGTAGAAACATTAACCATATTACTAATTGATTGATCAGGTCCTAGGTAACAAATACCAGAGCAAGTTGCTTGAAATCATCGATGCCAGGTCTATATTTTCTTCTTTATCATTGTAGTATTTTTTTTCTCTTTTTCTCATTAATCAAATTGATTAGTAAGCATGCTCTTTCAATCCATCCATATGTCACATAATACGAATAGCATTACTACTTAGTATTTAACTCCTTGTGTTTTTTGGGTTAACAGTGAACTTCCAGAGTTCTTGGGGGGTACTTGTACTTGTGCAGACAAAGGTGGTTGCATGCTTTCTGACAAGGGACCATGGAATGATCCAGAAATCTTGAAGGTAGTTTCACGGTAGAAAGATATAAAAAAAAATTGTTGAATAATTAGAAGAAAAAATAAATAGTGAAGTGTTTTTTTTTCCTGATGAATTAGATGGTTCAAAACGGTGAAGCAAAATGCAGAAGGAAAACTTTATCCGAAGTTGAGGAGAAAACAATCGTCGAAGACGAAGGAGTGTGTCAAAAGGTAGTTATCTTCCTACTTCTTTATAGTTTCAAGAGCATAGTTTGGTTATGAAACTTGATGTCCTTTCTTTTTCTTATGATATTCAGGGATGTGATTCTTTCAACAAAGAATACGTTCTCGAGGAGGCATGCCATGCTATAGCAGAAGTAAGTTTCATTACTTGATAAAATGTAAAACAAATGATGATTTCATATTCATTCCACTCACTTTCAATTTTGCAGGTAGCAAAACAATGTAATGCATATCAGTTCGACACCTTGGTCTCAATGAATGACAAACAAGTATCTTGGGAGGAATCAGTACAAAATGATCAAAAGGCACTTTCTAAAAGTATTTGATACTGTTCTGTGATTATTTTTACAGACAAATTTATTTTGTCAAGCATAATCAGCATACTCCATTTTATCAACACAGTAAAGAATGACTTAGATAAAATACTAAATAGATCATTTCCGTTGATGTGCAGATTGCTTTGGTAACAATGTATGCAAGAATGGATTTAGAAATCAATTTTCCGGCAGAATTATGGCCATGGTTTTGGGAATCATGACGTTCATCCGCATGACACGGAGTATACCAACAAAAATGACTGCAATTGCATTGTATGGTAACTCGGTATATATTGATGGCAATATGGTGAAAGCAGCACCTGTCATTTCCGTTGATGATCACATGGCTCTAATGAAGCGCATGACCGATCTAGAAGAGAAGGTGAATATTCTCAGCATGAGACCTTCCATGCCACCTGAAATGGAGGAATTGCTCAACAGTACTCTAAACCGTGTCGATACACTGGAGCAAGAGTTGGCTACAGCCAAGAAGGTACTGTTTTTCTTGCTACTTCACAATTTTATAATAATTTCAGAACATATTTGTAATTTATTTGTTCCTTTTTATTGGCATTAGGCACTTGATGATGCCCTTGCTAGGCAAGTGGAGCTTCAATCACATATCGACAAAAAGAAAAAGAAGAAAAAGTTGGTAGGTCATTTTAACTTTGACAATGATACATTACTTACCATGCATTGTTTCTATAAATATATACTTTCTTTTTATCTTATACAAGTTTCTGTGTTTGTGATTGCAGTTCCACTGGTAA
Protein sequence

>MS.gene50751.t1

MCDTMSGPITIDQPVKGLEIEYLEDEKRTRLGSLKKAAISASSKFKHSFKKGRRHNRVISVIEDEIDTEELQAIDAFRQTLILEELLPSKHDDHHMMLRFLRARKFDIEKTKQMWADMLQWRREFGADTIMEDFEFEEIDEVLKYYPQGTHGVDKDGRPVYIERLGLVDSNKLMQVTTMDRYLKYHVREFEKTSNVKMPACSIAAKKHIDQSTTILDVQGVGLKSMNKAARDLIQGLQKIDGDNYPESLNRMFIINAGSGFRILWNTVKSFLDPKTTSKIHVLGNKYQSKLLEIIDASELPEFLGGTCTCADKGGCMLSDKGPWNDPEILKMVQNGEAKCRRKTLSEVEEKTIVEDEGVCQKGCDSFNKEYVLEEACHAIAEVAKQCNAYQFDTLVSMNDKQVSWEESVQNDQKALSKNCFGNNVCKNGFRNQFSGRIMAMVLGIMTFIRMTRSIPTKMTAIALYGNSVYIDGNMVKAAPVISVDDHMALMKRMTDLEEKVNILSMRPSMPPEMEELLNSTLNRVDTLEQELATAKKALDDALARQVELQSHIDKKKKKKKLFHW