AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51172


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51172.t1 MTR_7g088160 43.465 329 147 3 34 337 75 389 1.67e-84 261
MS.gene51172.t1 MTR_4g063670 43.750 320 149 9 31 336 17 319 1.80e-63 204
MS.gene51172.t1 MTR_0867s0010 44.062 320 148 8 31 336 17 319 7.13e-60 195
MS.gene51172.t1 MTR_4g083510 39.763 337 169 10 16 336 2 320 9.00e-52 174
MS.gene51172.t1 MTR_7g107340 36.513 304 145 4 32 334 76 332 4.72e-51 172
MS.gene51172.t1 MTR_1g051185 47.682 151 69 4 93 241 45 187 4.52e-36 131
MS.gene51172.t1 MTR_3g069460 42.771 166 84 3 31 194 17 173 9.60e-36 128
MS.gene51172.t1 MTR_7g114170 32.013 303 130 9 32 334 61 287 2.17e-31 119
MS.gene51172.t1 MTR_6g081150 36.413 184 71 5 93 276 40 177 1.01e-24 99.0
MS.gene51172.t1 MTR_5g066280 24.503 302 200 5 36 335 55 330 4.28e-22 95.5
MS.gene51172.t1 MTR_4g026120 24.503 302 200 5 36 335 55 330 1.22e-21 94.4
MS.gene51172.t1 MTR_4g036435 24.503 302 200 5 36 335 55 330 1.22e-21 94.4
MS.gene51172.t1 MTR_3g067785 24.503 302 200 5 36 335 55 330 1.25e-21 94.4
MS.gene51172.t1 MTR_4g013220 24.503 302 200 5 36 335 55 330 1.40e-21 94.0
MS.gene51172.t1 MTR_1g106885 24.503 302 200 5 36 335 116 391 5.74e-21 93.2
MS.gene51172.t1 MTR_2g022120 24.503 302 200 5 36 335 119 394 6.00e-21 93.2
MS.gene51172.t1 MTR_5g077245 24.172 302 201 5 36 335 108 383 3.96e-20 90.5
MS.gene51172.t1 MTR_2g072860 28.659 164 112 2 34 197 29 187 5.72e-19 86.3
MS.gene51172.t1 MTR_7g461350 27.551 196 132 4 1 196 69 254 7.71e-19 86.7
MS.gene51172.t1 MTR_6g016670 27.551 196 132 4 1 196 69 254 7.71e-19 86.7
MS.gene51172.t1 MTR_3g464310 27.551 196 132 4 1 196 69 254 7.71e-19 86.7
MS.gene51172.t1 MTR_6g092533 27.329 161 114 1 36 196 119 276 1.00e-18 86.7
MS.gene51172.t1 MTR_3g464170 27.041 196 133 4 1 196 69 254 1.38e-18 85.9
MS.gene51172.t1 MTR_1g100375 27.041 196 133 4 1 196 69 254 1.38e-18 85.9
MS.gene51172.t1 MTR_6g051405 28.834 163 111 2 34 196 97 254 1.52e-18 85.9
MS.gene51172.t1 MTR_3g450670 28.221 163 112 2 34 196 93 250 4.15e-18 84.7
MS.gene51172.t1 MTR_1g471070 28.221 163 112 2 34 196 93 250 4.15e-18 84.7
MS.gene51172.t1 MTR_7g062380 23.977 171 112 1 75 245 26 178 7.12e-15 73.9
MS.gene51172.t1 MTR_6g453050 24.262 305 188 9 9 304 35 305 7.06e-14 71.6
MS.gene51172.t1 MTR_8g015740 37.931 116 56 3 2 105 19 130 1.94e-13 67.0
MS.gene51172.t1 MTR_8g015750 33.645 214 94 8 141 336 3 186 6.53e-13 67.0
MS.gene51172.t1 MTR_7g083520 23.125 160 120 1 38 197 31 187 8.57e-13 68.2
MS.gene51172.t1 MTR_4g046090 26.316 152 94 1 95 246 71 204 1.18e-12 67.4
MS.gene51172.t1 MTR_1g086750 27.966 118 85 0 75 192 26 143 1.58e-12 66.2
MS.gene51172.t1 MTR_4g046740 35.659 129 64 5 212 336 23 136 1.88e-12 64.3
MS.gene51172.t1 MTR_2g023560 63.636 44 16 0 74 117 62 105 3.67e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51172.t1 AT5G41220 38.693 199 107 5 66 254 206 399 9.69e-33 127
MS.gene51172.t1 AT5G41220 38.693 199 107 5 66 254 214 407 1.11e-32 127
MS.gene51172.t1 AT5G41220 38.693 199 107 5 66 254 264 457 1.12e-32 128
MS.gene51172.t1 AT5G41240 37.438 203 111 5 66 257 264 461 1.23e-31 125
MS.gene51172.t1 AT3G47680 32.093 215 118 8 38 243 14 209 2.66e-23 98.2
MS.gene51172.t1 AT1G66235 25.907 193 122 5 75 252 7 193 6.47e-13 68.6

Find 56 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGATTCTTCAGTAAAT+TGG 0.187266 7.3:+27481278 None:intergenic
TAGAAATATTTAACCATGAT+TGG 0.280172 7.3:+27481611 None:intergenic
ATCATGGTTAAATATTTCTA+AGG 0.308295 7.3:-27481608 MS.gene51172:CDS
GTAAATTGGCACGACCATTT+AGG 0.320469 7.3:+27481292 None:intergenic
TACTAAAAGATGAACCTAAA+TGG 0.349471 7.3:-27481306 MS.gene51172:CDS
AGAACAAAGAATTCTGCTTC+AGG 0.392497 7.3:-27481253 MS.gene51172:CDS
AGAATTCTTTGTTCTCTTGT+TGG 0.394331 7.3:+27481261 None:intergenic
TGATCAAATGTGGGGATAGA+AGG 0.401094 7.3:+27481847 None:intergenic
GTTAGATCCACATGAACAAT+AGG 0.411169 7.3:+27481091 None:intergenic
TGAGCTTCACTATCTCTTGA+TGG 0.426003 7.3:-27480954 MS.gene51172:CDS
TCACCACTTGTAGGCGTGTC+CGG 0.436446 7.3:+27481208 None:intergenic
AGTAGAGTGGTTCGTCCGAT+TGG 0.444657 7.3:-27481160 MS.gene51172:CDS
ATCTCTTGATGGATACATCT+AGG 0.458045 7.3:-27480943 MS.gene51172:CDS
ATACACTTCTTATCCAATCA+TGG 0.460631 7.3:-27481624 MS.gene51172:CDS
TTTGAAGAAATCTGAGCTGT+TGG 0.469253 7.3:+27481700 None:intergenic
ATGGTCGAAGGTGAACTTGA+TGG 0.477920 7.3:-27481470 MS.gene51172:CDS
CTACACATCCTCTTCTAATC+CGG 0.479246 7.3:-27481227 MS.gene51172:CDS
ATTTCTAAGGATCCAATTGT+GGG 0.492615 7.3:-27481595 MS.gene51172:CDS
AGGAGAAAATATATAAAGAA+AGG 0.495810 7.3:-27481663 MS.gene51172:CDS
GTAGAGTGGTTCGTCCGATT+GGG 0.497308 7.3:-27481159 MS.gene51172:CDS
GAAGGTGAGGATTGAAACAT+AGG 0.501482 7.3:+27481865 None:intergenic
TCTATCCCCACATTTGATCA+AGG 0.504348 7.3:-27481844 MS.gene51172:CDS
TTGTTCATGTGGATCTAACT+AGG 0.507003 7.3:-27481087 MS.gene51172:CDS
GACAAAAGCAGCTAAAAGAA+AGG 0.508350 7.3:-27481137 MS.gene51172:CDS
TATTTCTAAGGATCCAATTG+TGG 0.512026 7.3:-27481596 MS.gene51172:CDS
TGAAGTTCCTTGATCAAATG+TGG 0.515631 7.3:+27481837 None:intergenic
CCAACTTGAGAGGAAAATGA+TGG 0.520919 7.3:+27481757 None:intergenic
GCATGAAGGTAACAAGTTGA+AGG 0.526954 7.3:-27481008 MS.gene51172:CDS
CGACATTATGCAAGATGCAT+TGG 0.532468 7.3:-27481383 MS.gene51172:CDS
ACAAAAGCAGCTAAAAGAAA+GGG 0.543467 7.3:-27481136 MS.gene51172:CDS
TGTCCGAGGTCACCACTTGT+AGG 0.544239 7.3:+27481199 None:intergenic
CACTTGAGTTGTATTGTCCG+AGG 0.553422 7.3:+27481185 None:intergenic
GCCTATTCAAGAAAGAAAAG+TGG 0.554764 7.3:-27481418 MS.gene51172:CDS
AGTTGGGTTAGATAATATTG+AGG 0.557841 7.3:-27481740 MS.gene51172:CDS
GAAGTTCCTTGATCAAATGT+GGG 0.558018 7.3:+27481838 None:intergenic
AAATGTGGGGATAGAAGGTG+AGG 0.558798 7.3:+27481852 None:intergenic
TGAATCAAATTAAATGTCGA+TGG 0.569722 7.3:-27481489 MS.gene51172:CDS
TCATGTGGATCTAACTAGGA+TGG 0.578161 7.3:-27481083 MS.gene51172:CDS
TGAAACACCTATTGTTCATG+TGG 0.578577 7.3:-27481098 MS.gene51172:CDS
AATTAAATGTCGATGGTCGA+AGG 0.587022 7.3:-27481482 MS.gene51172:CDS
CGGATTAGAAGAGGATGTGT+AGG 0.590924 7.3:+27481228 None:intergenic
ATTATCTAACCCAACTTGAG+AGG 0.596041 7.3:+27481747 None:intergenic
TTCTAAGGATCCAATTGTGG+GGG 0.599880 7.3:-27481593 MS.gene51172:CDS
ATTGCACGTGGTGTGCATGA+AGG 0.607967 7.3:-27481022 MS.gene51172:CDS
GATAATTATAATCAATATCG+TGG 0.614936 7.3:-27481532 MS.gene51172:CDS
ACGCCTACAAGTGGTGACCT+CGG 0.622408 7.3:-27481202 MS.gene51172:CDS
AATCCGGACACGCCTACAAG+TGG 0.635438 7.3:-27481211 MS.gene51172:CDS
AGGCGTGTCCGGATTAGAAG+AGG 0.638071 7.3:+27481219 None:intergenic
TTTCTAAGGATCCAATTGTG+GGG 0.639337 7.3:-27481594 MS.gene51172:CDS
AAGTTCCTTGATCAAATGTG+GGG 0.640420 7.3:+27481839 None:intergenic
CAACTCAAGTGAAAGTAGAG+TGG 0.640911 7.3:-27481173 MS.gene51172:CDS
ATATAAAGAAAGGTTTACCA+CGG 0.691861 7.3:-27481653 MS.gene51172:CDS
GCTTTAGAAGATATTGCACG+TGG 0.699659 7.3:-27481034 MS.gene51172:CDS
TAAAGAAAGGTTTACCACGG+AGG 0.701400 7.3:-27481650 MS.gene51172:CDS
GGATACATCTAGGATGACAG+AGG 0.702928 7.3:-27480933 MS.gene51172:CDS
AAGAAGTGTATCTTCCTCCG+TGG 0.715732 7.3:+27481636 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGAGAAAATATATAAAGAA+AGG - chr7.3:27481070-27481089 MS.gene51172:CDS 20.0%
!! GATAATTATAATCAATATCG+TGG - chr7.3:27481201-27481220 MS.gene51172:CDS 20.0%
!! TAGAAATATTTAACCATGAT+TGG + chr7.3:27481125-27481144 None:intergenic 20.0%
!! TTCAAAAGAAACAAAAACAA+AGG - chr7.3:27481050-27481069 MS.gene51172:CDS 20.0%
!!! ATCATGGTTAAATATTTCTA+AGG - chr7.3:27481125-27481144 MS.gene51172:CDS 20.0%
! ATATAAAGAAAGGTTTACCA+CGG - chr7.3:27481080-27481099 MS.gene51172:CDS 25.0%
! GGAAAAAGAATTGCAAATTA+AGG - chr7.3:27481746-27481765 MS.gene51172:CDS 25.0%
! TACTAAAAGATGAACCTAAA+TGG - chr7.3:27481427-27481446 MS.gene51172:CDS 25.0%
! TGAATCAAATTAAATGTCGA+TGG - chr7.3:27481244-27481263 MS.gene51172:CDS 25.0%
ACAAAAGCAGCTAAAAGAAA+GGG - chr7.3:27481597-27481616 MS.gene51172:CDS 30.0%
AGAATTCTTTGTTCTCTTGT+TGG + chr7.3:27481475-27481494 None:intergenic 30.0%
ATACACTTCTTATCCAATCA+TGG - chr7.3:27481109-27481128 MS.gene51172:CDS 30.0%
TGTTGGATTCTTCAGTAAAT+TGG + chr7.3:27481458-27481477 None:intergenic 30.0%
! AGTTGGGTTAGATAATATTG+AGG - chr7.3:27480993-27481012 MS.gene51172:CDS 30.0%
! ATTTCTAAGGATCCAATTGT+GGG - chr7.3:27481138-27481157 MS.gene51172:CDS 30.0%
! TATTTCTAAGGATCCAATTG+TGG - chr7.3:27481137-27481156 MS.gene51172:CDS 30.0%
! TCTTTTAGTAATTTCCATGC+TGG + chr7.3:27481417-27481436 None:intergenic 30.0%
!! ACCACTTTTCTTTCTTGAAT+AGG + chr7.3:27481319-27481338 None:intergenic 30.0%
AAGTTCCTTGATCAAATGTG+GGG + chr7.3:27480897-27480916 None:intergenic 35.0%
AATTAAATGTCGATGGTCGA+AGG - chr7.3:27481251-27481270 MS.gene51172:CDS 35.0%
AGAACAAAGAATTCTGCTTC+AGG - chr7.3:27481480-27481499 MS.gene51172:CDS 35.0%
ATTATCTAACCCAACTTGAG+AGG + chr7.3:27480989-27481008 None:intergenic 35.0%
GAAGTTCCTTGATCAAATGT+GGG + chr7.3:27480898-27480917 None:intergenic 35.0%
GACAAAAGCAGCTAAAAGAA+AGG - chr7.3:27481596-27481615 MS.gene51172:CDS 35.0%
GCCTATTCAAGAAAGAAAAG+TGG - chr7.3:27481315-27481334 MS.gene51172:CDS 35.0%
GTTAGATCCACATGAACAAT+AGG + chr7.3:27481645-27481664 None:intergenic 35.0%
TGAAACACCTATTGTTCATG+TGG - chr7.3:27481635-27481654 MS.gene51172:CDS 35.0%
TGAAGTTCCTTGATCAAATG+TGG + chr7.3:27480899-27480918 None:intergenic 35.0%
TTGTTCATGTGGATCTAACT+AGG - chr7.3:27481646-27481665 MS.gene51172:CDS 35.0%
TTTGAAGAAATCTGAGCTGT+TGG + chr7.3:27481036-27481055 None:intergenic 35.0%
! ACCAAAGAGCAAGTTCTTTT+TGG - chr7.3:27481166-27481185 MS.gene51172:CDS 35.0%
! ATCTCTTGATGGATACATCT+AGG - chr7.3:27481790-27481809 MS.gene51172:CDS 35.0%
! CATCATTTTCCTCTCAAGTT+GGG - chr7.3:27480977-27480996 MS.gene51172:CDS 35.0%
! TCCAAAAAGAACTTGCTCTT+TGG + chr7.3:27481170-27481189 None:intergenic 35.0%
! TTTCTAAGGATCCAATTGTG+GGG - chr7.3:27481139-27481158 MS.gene51172:CDS 35.0%
CAACTCAAGTGAAAGTAGAG+TGG - chr7.3:27481560-27481579 MS.gene51172:CDS 40.0%
CCAACTTGAGAGGAAAATGA+TGG + chr7.3:27480979-27480998 None:intergenic 40.0%
CGACATTATGCAAGATGCAT+TGG - chr7.3:27481350-27481369 MS.gene51172:CDS 40.0%
CTACACATCCTCTTCTAATC+CGG - chr7.3:27481506-27481525 MS.gene51172:CDS 40.0%
GTAAATTGGCACGACCATTT+AGG + chr7.3:27481444-27481463 None:intergenic 40.0%
TAAAGAAAGGTTTACCACGG+AGG - chr7.3:27481083-27481102 MS.gene51172:CDS 40.0%
TCATGTGGATCTAACTAGGA+TGG - chr7.3:27481650-27481669 MS.gene51172:CDS 40.0%
TCTATCCCCACATTTGATCA+AGG - chr7.3:27480889-27480908 MS.gene51172:CDS 40.0%
TGAGCTTCACTATCTCTTGA+TGG - chr7.3:27481779-27481798 MS.gene51172:CDS 40.0%
TGATCAAATGTGGGGATAGA+AGG + chr7.3:27480889-27480908 None:intergenic 40.0%
! CCATCATTTTCCTCTCAAGT+TGG - chr7.3:27480976-27480995 MS.gene51172:CDS 40.0%
! GCATGAAGGTAACAAGTTGA+AGG - chr7.3:27481725-27481744 MS.gene51172:CDS 40.0%
! GTTTTATGTTAGTGCCAGCA+TGG - chr7.3:27481400-27481419 MS.gene51172:CDS 40.0%
! TTCTAAGGATCCAATTGTGG+GGG - chr7.3:27481140-27481159 MS.gene51172:CDS 40.0%
!! GCTTTAGAAGATATTGCACG+TGG - chr7.3:27481699-27481718 MS.gene51172:CDS 40.0%
!!! TTAGCTGCTTTTGTCCCAAT+CGG + chr7.3:27481591-27481610 None:intergenic 40.0%
AAATGTGGGGATAGAAGGTG+AGG + chr7.3:27480884-27480903 None:intergenic 45.0%
ATGGTCGAAGGTGAACTTGA+TGG - chr7.3:27481263-27481282 MS.gene51172:CDS 45.0%
! AAGAAGTGTATCTTCCTCCG+TGG + chr7.3:27481100-27481119 None:intergenic 45.0%
! CACTTGAGTTGTATTGTCCG+AGG + chr7.3:27481551-27481570 None:intergenic 45.0%
! CGGATTAGAAGAGGATGTGT+AGG + chr7.3:27481508-27481527 None:intergenic 45.0%
! GGATACATCTAGGATGACAG+AGG - chr7.3:27481800-27481819 MS.gene51172:CDS 45.0%
AGTAGAGTGGTTCGTCCGAT+TGG - chr7.3:27481573-27481592 MS.gene51172:CDS 50.0%
ATTGCACGTGGTGTGCATGA+AGG - chr7.3:27481711-27481730 MS.gene51172:CDS 50.0%
GTAGAGTGGTTCGTCCGATT+GGG - chr7.3:27481574-27481593 MS.gene51172:CDS 50.0%
!! CTTTGGTTTGCCCCCACAAT+TGG + chr7.3:27481153-27481172 None:intergenic 50.0%
AATCCGGACACGCCTACAAG+TGG - chr7.3:27481522-27481541 MS.gene51172:CDS 55.0%
ACGCCTACAAGTGGTGACCT+CGG - chr7.3:27481531-27481550 MS.gene51172:CDS 55.0%
TCACCACTTGTAGGCGTGTC+CGG + chr7.3:27481528-27481547 None:intergenic 55.0%
TGTCCGAGGTCACCACTTGT+AGG + chr7.3:27481537-27481556 None:intergenic 55.0%
! AGGCGTGTCCGGATTAGAAG+AGG + chr7.3:27481517-27481536 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 27480871 27481884 27480871 ID=MS.gene51172
chr7.3 mRNA 27480871 27481884 27480871 ID=MS.gene51172.t1;Parent=MS.gene51172
chr7.3 exon 27480871 27481884 27480871 ID=MS.gene51172.t1.exon1;Parent=MS.gene51172.t1
chr7.3 CDS 27480871 27481884 27480871 ID=cds.MS.gene51172.t1;Parent=MS.gene51172.t1
Gene Sequence

>MS.gene51172

ATGTTTCAATCCTCACCTTCTATCCCCACATTTGATCAAGGAACTTCAATGAGTATTGCTACAGAACAAGAAACAACATCTCAAGCGTCTCAACAAATTCAAAATCCATCATTTTCCTCTCAAGTTGGGTTAGATAATATTGAGGTTAATGATGAAGAAGAACCAACAGCTCAGATTTCTTCAAAAGAAACAAAAACAAAGGAGAAAATATATAAAGAAAGGTTTACCACGGAGGAAGATACACTTCTTATCCAATCATGGTTAAATATTTCTAAGGATCCAATTGTGGGGGCAAACCAAAGAGCAAGTTCTTTTTGGAAGAGAATTACAGATAATTATAATCAATATCGTGGTCATTTGAAAGAGAGAGAGTTGAATCAAATTAAATGTCGATGGTCGAAGGTGAACTTGATGGTTCATAAATTTGCAACTTGCTACAAACAAGCCTATTCAAGAAAGAAAAGTGGTAGCTCTGAAGACGACATTATGCAAGATGCATTGGCAATTTATGCTCATGATAAGCGTTCACGTTTTATGTTAGTGCCAGCATGGAAATTACTAAAAGATGAACCTAAATGGTCGTGCCAATTTACTGAAGAATCCAACAAGAGAACAAAGAATTCTGCTTCAGGAGCCTACACATCCTCTTCTAATCCGGACACGCCTACAAGTGGTGACCTCGGACAATACAACTCAAGTGAAAGTAGAGTGGTTCGTCCGATTGGGACAAAAGCAGCTAAAAGAAAGGGAAAATCAAAAGCAAATGAAACACCTATTGTTCATGTGGATCTAACTAGGATGGAAGATGCACAACAATCAAAAGCTCTAGCTTTAGAAGATATTGCACGTGGTGTGCATGAAGGTAACAAGTTGAAGGAAAAAGAATTGCAAATTAAGGAAAGAAATGATGAGCTTCACTATCTCTTGATGGATACATCTAGGATGACAGAGGAACAAGTGCAAGCTCATAAAGTGTTGTGTAAAATTATTAGAGAAAAATATGAACTTGTGTAA

Protein sequence

>MS.gene51172.t1

MFQSSPSIPTFDQGTSMSIATEQETTSQASQQIQNPSFSSQVGLDNIEVNDEEEPTAQISSKETKTKEKIYKERFTTEEDTLLIQSWLNISKDPIVGANQRASSFWKRITDNYNQYRGHLKERELNQIKCRWSKVNLMVHKFATCYKQAYSRKKSGSSEDDIMQDALAIYAHDKRSRFMLVPAWKLLKDEPKWSCQFTEESNKRTKNSASGAYTSSSNPDTPTSGDLGQYNSSESRVVRPIGTKAAKRKGKSKANETPIVHVDLTRMEDAQQSKALALEDIARGVHEGNKLKEKELQIKERNDELHYLLMDTSRMTEEQVQAHKVLCKIIREKYELV