AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51353

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51353.t1 MTR_4g045853 96.552 290 10 0 1 290 5 294 0.0 595
MS.gene51353.t1 MTR_2g089140 79.931 289 57 1 1 289 4 291 0.0 501
MS.gene51353.t1 MTR_2g089140 82.456 228 40 0 62 289 1 228 3.60e-149 417
MS.gene51353.t1 MTR_7g084750 49.660 294 132 4 3 288 6 291 1.09e-102 301
MS.gene51353.t1 MTR_7g084770 50.000 292 126 4 9 288 8 291 3.88e-102 300
MS.gene51353.t1 MTR_7g084760 50.000 290 129 4 3 284 6 287 1.27e-101 299
MS.gene51353.t1 MTR_6g033055 49.153 295 129 4 7 288 5 291 2.02e-101 298
MS.gene51353.t1 MTR_4g097640 50.877 285 128 4 4 285 13 288 2.45e-101 298
MS.gene51353.t1 MTR_4g097670 50.877 285 128 4 4 285 13 288 2.45e-101 298
MS.gene51353.t1 MTR_6g033085 48.822 297 131 4 5 288 3 291 1.10e-100 296
MS.gene51353.t1 MTR_4g097700 52.000 275 120 4 14 285 60 325 2.82e-99 294
MS.gene51353.t1 MTR_4g128410 48.944 284 133 4 4 284 6 280 1.23e-96 286
MS.gene51353.t1 MTR_3g084620 49.296 284 124 5 9 288 14 281 1.86e-95 283
MS.gene51353.t1 MTR_3g084630 49.296 284 124 5 9 288 14 281 2.29e-95 283
MS.gene51353.t1 MTR_7g093530 48.936 282 132 4 7 285 8 280 5.06e-94 279
MS.gene51353.t1 MTR_8g081040 47.183 284 132 4 7 286 14 283 2.13e-92 275
MS.gene51353.t1 MTR_4g128580 49.077 271 130 4 19 286 22 287 1.43e-90 271
MS.gene51353.t1 MTR_2g038860 46.690 287 145 4 6 288 8 290 8.22e-90 269
MS.gene51353.t1 MTR_2g038705 46.690 287 145 4 6 288 8 290 8.22e-90 269
MS.gene51353.t1 MTR_4g126920 48.718 273 129 4 15 284 17 281 1.44e-89 268
MS.gene51353.t1 MTR_1g105900 44.948 287 145 5 4 284 12 291 2.17e-89 268
MS.gene51353.t1 MTR_4g128570 47.586 290 115 6 10 285 7 273 2.89e-89 267
MS.gene51353.t1 MTR_6g088320 43.972 282 152 2 4 284 7 283 2.66e-88 265
MS.gene51353.t1 MTR_1g105495 46.021 289 138 5 7 284 13 294 1.21e-87 264
MS.gene51353.t1 MTR_4g128400 47.656 256 124 3 36 290 43 289 3.05e-84 254
MS.gene51353.t1 MTR_8g102910 45.196 281 147 4 6 284 4 279 3.30e-84 254
MS.gene51353.t1 MTR_8g102220 43.986 291 125 7 10 290 10 272 2.22e-81 247
MS.gene51353.t1 MTR_4g128590 43.110 283 138 4 7 286 15 277 4.93e-78 238
MS.gene51353.t1 MTR_8g013055 42.105 266 143 4 23 286 31 287 6.06e-77 236
MS.gene51353.t1 MTR_5g091730 42.857 287 136 6 7 284 11 278 1.67e-75 232
MS.gene51353.t1 MTR_3g089540 44.867 263 130 5 23 284 34 282 5.64e-75 231
MS.gene51353.t1 MTR_7g056090 41.758 273 144 6 4 273 13 273 2.89e-66 209
MS.gene51353.t1 MTR_2g049030 38.610 259 142 8 23 278 30 274 1.98e-58 188
MS.gene51353.t1 MTR_2g049130 39.130 253 139 7 23 272 29 269 6.77e-57 184
MS.gene51353.t1 MTR_1g033420 37.037 297 164 8 1 285 7 292 1.80e-56 185
MS.gene51353.t1 MTR_7g102260 37.050 278 147 6 20 284 29 291 2.20e-55 181
MS.gene51353.t1 MTR_5g029100 39.044 251 143 5 39 284 60 305 2.30e-55 182
MS.gene51353.t1 MTR_1g063020 39.431 246 126 5 47 284 64 294 2.10e-54 179
MS.gene51353.t1 MTR_8g090100 37.402 254 142 6 39 285 39 282 3.74e-53 176
MS.gene51353.t1 MTR_2g095800 36.201 279 155 6 14 284 46 309 7.49e-53 175
MS.gene51353.t1 MTR_4g057450 35.878 262 145 5 23 276 50 296 3.46e-52 173
MS.gene51353.t1 MTR_5g029100 39.241 237 134 5 53 284 43 274 5.23e-52 173
MS.gene51353.t1 MTR_8g064180 34.496 258 152 5 36 284 61 310 1.70e-50 169
MS.gene51353.t1 MTR_8g479340 41.176 102 53 2 183 284 41 135 4.51e-21 87.4
MS.gene51353.t1 MTR_8g032640 31.176 170 109 4 43 210 33 196 4.48e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51353.t1 AT4G03210 69.310 290 76 3 7 289 6 289 3.21e-156 437
MS.gene51353.t1 AT4G03210 73.840 237 56 2 53 289 31 261 3.41e-138 390
MS.gene51353.t1 AT5G13870 50.916 273 124 3 18 288 27 291 7.82e-102 300
MS.gene51353.t1 AT5G13870 50.916 273 124 3 18 288 27 291 7.82e-102 300
MS.gene51353.t1 AT5G13870 50.916 273 124 3 18 288 27 291 7.82e-102 300
MS.gene51353.t1 AT4G37800 50.174 287 128 5 4 284 12 289 3.52e-100 295
MS.gene51353.t1 AT5G65730 50.000 284 129 5 4 284 17 290 1.36e-98 291
MS.gene51353.t1 AT3G23730 49.825 285 133 4 7 288 13 290 3.31e-97 288
MS.gene51353.t1 AT2G06850 48.519 270 129 3 23 290 35 296 1.11e-93 279
MS.gene51353.t1 AT4G14130 50.174 287 127 7 9 288 11 288 1.48e-93 278
MS.gene51353.t1 AT4G25810 46.290 283 141 5 7 285 9 284 1.65e-90 271
MS.gene51353.t1 AT5G57530 48.708 271 126 6 17 284 22 282 2.81e-90 270
MS.gene51353.t1 AT5G57540 50.598 251 114 4 35 284 40 281 2.87e-90 270
MS.gene51353.t1 AT5G57550 46.392 291 135 5 5 286 6 284 5.29e-90 269
MS.gene51353.t1 AT5G57560 44.746 295 138 5 1 285 3 282 9.02e-90 268
MS.gene51353.t1 AT1G11545 47.458 295 135 7 1 284 14 299 9.09e-89 267
MS.gene51353.t1 AT4G28850 45.230 283 144 3 9 284 9 287 1.95e-87 263
MS.gene51353.t1 AT2G06850 49.378 241 114 2 50 290 4 236 2.15e-87 261
MS.gene51353.t1 AT2G14620 45.105 286 144 5 5 284 16 294 9.41e-87 261
MS.gene51353.t1 AT4G25820 46.886 273 133 4 15 284 22 285 1.15e-84 256
MS.gene51353.t1 AT4G30270 43.617 282 133 5 7 285 8 266 3.12e-84 254
MS.gene51353.t1 AT2G14620 48.291 234 113 3 52 284 18 244 1.17e-81 247
MS.gene51353.t1 AT2G18800 44.257 296 145 7 1 285 11 297 4.99e-81 247
MS.gene51353.t1 AT5G48070 46.748 246 117 4 40 284 49 281 2.65e-79 242
MS.gene51353.t1 AT2G14620 48.444 225 108 3 61 284 16 233 3.92e-79 240
MS.gene51353.t1 AT4G13090 41.781 292 155 5 1 284 4 288 1.09e-78 241
MS.gene51353.t1 AT3G25050 43.182 264 139 5 23 284 35 289 1.61e-76 235
MS.gene51353.t1 AT4G13080 44.318 264 134 5 23 284 39 291 1.00e-72 226
MS.gene51353.t1 AT4G30290 44.017 234 117 4 52 284 56 276 3.74e-70 218
MS.gene51353.t1 AT1G65310 43.404 235 119 4 52 285 61 282 4.11e-69 216
MS.gene51353.t1 AT4G30280 44.017 234 117 4 52 284 61 281 4.29e-69 216
MS.gene51353.t1 AT1G65310 43.404 235 119 4 52 285 83 304 6.56e-69 216
MS.gene51353.t1 AT3G44990 39.560 273 142 5 20 284 36 293 2.97e-60 193
MS.gene51353.t1 AT1G32170 38.776 294 158 9 5 285 10 294 1.03e-59 194
MS.gene51353.t1 AT1G14720 36.054 294 164 8 4 285 10 291 7.52e-56 183
MS.gene51353.t1 AT2G01850 36.986 292 166 7 1 285 11 291 2.43e-55 182
MS.gene51353.t1 AT2G36870 37.591 274 146 7 20 284 40 297 2.24e-54 179
MS.gene51353.t1 AT1G10550 36.759 253 149 5 40 284 60 309 4.74e-54 178
MS.gene51353.t1 AT4G18990 35.636 275 152 6 32 285 43 313 2.67e-52 175
MS.gene51353.t1 AT3G48580 37.603 242 128 8 43 284 55 273 7.28e-48 161
MS.gene51353.t1 AT3G48580 36.752 234 125 8 51 284 4 214 2.80e-45 152
MS.gene51353.t1 AT4G18990 36.275 204 116 4 92 285 40 239 8.57e-42 145
MS.gene51353.t1 AT3G48580 31.405 242 123 8 43 284 55 253 2.31e-31 117
MS.gene51353.t1 AT2G36870 40.288 139 75 2 20 150 40 178 4.14e-28 107

Find 70 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTGTCAAGTTTGAGTTTA+AGG 0.041776 4.1:+51047269 None:intergenic
TTGGAAGAGTTCATCAAATT+TGG 0.187679 4.1:+51047333 None:intergenic
ATTGATAACAAAGGCTTTAT+AGG 0.203191 4.1:+51044552 None:intergenic
ATGTTTGTGTGTACCCTTAT+TGG 0.226363 4.1:+51044706 None:intergenic
CCCAAACATATACTTGCTTT+TGG 0.249364 4.1:+51046963 None:intergenic
ATTGGTTGGTCCTTTGGATA+TGG 0.256401 4.1:+51044667 None:intergenic
ACATTTGTTTGCACAGAATA+AGG 0.263774 4.1:+51046165 None:intergenic
GGCTTTATAGGTTGCAATAA+AGG 0.269483 4.1:+51044564 None:intergenic
ATTATTGTAGTGATGGTTCT+AGG 0.279226 4.1:-51044369 MS.gene51353:CDS
ACTCCCATTGGTTGGTCCTT+TGG 0.294306 4.1:+51044661 None:intergenic
TCCAAAAGCAAGTATATGTT+TGG 0.311980 4.1:-51046964 MS.gene51353:CDS
TAGTGGATGAAACACCAATA+AGG 0.312627 4.1:-51044720 MS.gene51353:CDS
TCCATTGCAGCCACTGAAAC+TGG 0.316411 4.1:+51044517 None:intergenic
ACACATTCAACAGGAGTAAT+AGG 0.318635 4.1:+51044343 None:intergenic
GATCTCCTTCATGGAGAAAA+TGG 0.350214 4.1:+51047293 None:intergenic
CTCAAACTTGACAACTCTTC+TGG 0.357467 4.1:-51047262 MS.gene51353:intron
AAATTAAGCACTGACAAATT+AGG 0.358680 4.1:+51044442 None:intergenic
AAATCAAACTCGTTGTGGTT+TGG 0.361017 4.1:+51046216 None:intergenic
GTTTAAGGAGATCTCCTTCA+TGG 0.368795 4.1:+51047284 None:intergenic
TGGGAGTATATAGTTCAATA+TGG 0.384156 4.1:-51044645 MS.gene51353:CDS
TGAACTCTTCCAACCAAGTT+GGG 0.405385 4.1:-51047323 MS.gene51353:CDS
AGTGGATGAAACACCAATAA+GGG 0.419179 4.1:-51044719 MS.gene51353:CDS
GCTTTATAGGTTGCAATAAA+GGG 0.421880 4.1:+51044565 None:intergenic
GTAATGAAGATAAGAAATAT+TGG 0.427156 4.1:-51044474 MS.gene51353:CDS
ACACACAAACATGGAACATA+AGG 0.435591 4.1:-51044695 MS.gene51353:CDS
GTGGGTTAGGAATCATCATA+TGG 0.442976 4.1:-51044398 MS.gene51353:CDS
CCAAAAGCAAGTATATGTTT+GGG 0.446940 4.1:-51046963 MS.gene51353:CDS
ATCAAAGTCATCAACTTATG+TGG 0.447459 4.1:-51044417 MS.gene51353:CDS
AATGTGTATGTGAATGGAAT+TGG 0.448488 4.1:-51046147 MS.gene51353:CDS
ATCCAGCTTAAGCTTGTTGA+AGG 0.448965 4.1:-51046931 MS.gene51353:CDS
GTCATCAACTTATGTGGGTT+AGG 0.449396 4.1:-51044411 MS.gene51353:CDS
GTGAGCAAAGACTTAACCTT+TGG 0.449716 4.1:-51046119 MS.gene51353:CDS
CAATATGGAATGCAGATGAT+TGG 0.451938 4.1:-51044630 MS.gene51353:CDS
AATATGGAATGCAGATGATT+GGG 0.477902 4.1:-51044629 MS.gene51353:CDS
CTTGTTGAAGGTGACTCTGC+TGG 0.484994 4.1:-51046919 MS.gene51353:CDS
CACCTTCAACAAGCTTAAGC+TGG 0.489730 4.1:+51046929 None:intergenic
CGATGATGGACACATTCAAC+AGG 0.489950 4.1:+51044334 None:intergenic
ATGAACTCTTCCAACCAAGT+TGG 0.492288 4.1:-51047324 MS.gene51353:CDS
CAAACAAATGTGTATGTGAA+TGG 0.495017 4.1:-51046153 MS.gene51353:CDS
TCCAGTTTCAGTGGCTGCAA+TGG 0.519854 4.1:-51044518 MS.gene51353:CDS
AGAGTAAGAGTGAAAATCCT+TGG 0.520212 4.1:+51046086 None:intergenic
GTTTATGATTATTGTAGTGA+TGG 0.523389 4.1:-51044376 MS.gene51353:CDS
GGTCAAAAGCCCAACTTGGT+TGG 0.526543 4.1:+51047314 None:intergenic
TAAGAGTGAAAATCCTTGGT+AGG 0.527500 4.1:+51046090 None:intergenic
ATCACTAGCTATGACGATGA+TGG 0.530048 4.1:+51044320 None:intergenic
CACACAAACATGGAACATAA+GGG 0.533729 4.1:-51044694 MS.gene51353:CDS
AACTATATACTCCCATTGGT+TGG 0.534934 4.1:+51044653 None:intergenic
ATTCAAAATCAAACTCGTTG+TGG 0.538842 4.1:+51046211 None:intergenic
ATTGAACTATATACTCCCAT+TGG 0.544274 4.1:+51044649 None:intergenic
TATCCAAAGGACCAACCAAT+GGG 0.553638 4.1:-51044664 MS.gene51353:CDS
GAGTTCATCAAATTTGGAAG+AGG 0.559328 4.1:+51047339 None:intergenic
AAATGGTCAAAAGCCCAACT+TGG 0.561534 4.1:+51047310 None:intergenic
GAGGAAGAGTGAAAACAGAT+TGG 0.562933 4.1:-51044597 MS.gene51353:CDS
TGAAGATAAGAAATATTGGT+GGG 0.579001 4.1:-51044470 MS.gene51353:CDS
AAAGGTTAAGTCTTTGCTCA+CGG 0.581166 4.1:+51046121 None:intergenic
TCAAAGTCATCAACTTATGT+GGG 0.588263 4.1:-51044416 MS.gene51353:CDS
ATGAAGATAAGAAATATTGG+TGG 0.588757 4.1:-51044471 MS.gene51353:CDS
AAGAGTGAAAATCCTTGGTA+GGG 0.591421 4.1:+51046091 None:intergenic
CCTTGGTAGGGTCAAACCAA+AGG 0.593403 4.1:+51046103 None:intergenic
TTGTGAGTGTCCAGTTTCAG+TGG 0.599803 4.1:-51044527 MS.gene51353:CDS
ATATCCAAAGGACCAACCAA+TGG 0.618347 4.1:-51044665 MS.gene51353:CDS
AATAAGGGTACACACAAACA+TGG 0.625212 4.1:-51044704 MS.gene51353:CDS
GCAGATGATTGGGCAACACA+AGG 0.626520 4.1:-51044619 MS.gene51353:CDS
GTGATATAGATGTCATCAGA+GGG 0.631940 4.1:-51046240 MS.gene51353:intron
CTCACAAGCATTGATAACAA+AGG 0.636656 4.1:+51044543 None:intergenic
CCTTTGGTTTGACCCTACCA+AGG 0.640836 4.1:-51046103 MS.gene51353:CDS
TAAGGGTATACCATATCCAA+AGG 0.644360 4.1:-51044677 MS.gene51353:CDS
TGTGATATAGATGTCATCAG+AGG 0.666933 4.1:-51046241 MS.gene51353:intron
CTGCAATGGATAATACAAAG+AGG 0.674798 4.1:-51044504 MS.gene51353:CDS
GATGATTGGGCAACACAAGG+AGG 0.684173 4.1:-51044616 MS.gene51353:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAAAAATATTACAATTT+AGG + chr4.1:51046758-51046777 None:intergenic 10.0%
!! CAATATTAATTCAATTAATT+AGG - chr4.1:51046866-51046885 MS.gene51353:intron 10.0%
!!! ACAAAAATATGTTTAAAATA+TGG + chr4.1:51044603-51044622 None:intergenic 10.0%
!!! ATTGAATATATTAAAGTATT+GGG + chr4.1:51046708-51046727 None:intergenic 10.0%
!!! TATTTTTCATAAAAAAAGAA+AGG - chr4.1:51046143-51046162 MS.gene51353:CDS 10.0%
!!! TCAAAAAATATTTTTACTAA+TGG + chr4.1:51046555-51046574 None:intergenic 10.0%
!!! TTTATTTTAATCTAATGTTA+AGG - chr4.1:51044993-51045012 MS.gene51353:intron 10.0%
!! AAGAGAATAAAATATAAGAA+TGG - chr4.1:51046168-51046187 MS.gene51353:CDS 15.0%
!! ACTTTAATATATTCAATGAT+TGG - chr4.1:51046711-51046730 MS.gene51353:intron 15.0%
!! AGAGAATAAAATATAAGAAT+GGG - chr4.1:51046169-51046188 MS.gene51353:CDS 15.0%
!! CATTGAATATATTAAAGTAT+TGG + chr4.1:51046709-51046728 None:intergenic 15.0%
!! TAATTAATTAACAATGACAA+TGG - chr4.1:51044709-51044728 MS.gene51353:CDS 15.0%
!! TTACTAAAGCAAATATTAAT+TGG + chr4.1:51045280-51045299 None:intergenic 15.0%
!! TTTATATTCATATTATTCTG+TGG - chr4.1:51045365-51045384 MS.gene51353:intron 15.0%
!!! AAAAAAGTCCATTTTTTTTT+TGG - chr4.1:51044908-51044927 MS.gene51353:intron 15.0%
!!! AAAAATATTACTTTTTGACA+AGG + chr4.1:51046011-51046030 None:intergenic 15.0%
!!! ATTTTTTAACCTTTATCATA+AGG - chr4.1:51046487-51046506 MS.gene51353:intron 15.0%
!!! CTTAGTTTAAAAAGATTTTA+AGG + chr4.1:51044539-51044558 None:intergenic 15.0%
!!! TATTTTGATCACTTATAAAA+TGG - chr4.1:51044627-51044646 MS.gene51353:CDS 15.0%
!!! TTGAATATATTAAAGTATTG+GGG + chr4.1:51046707-51046726 None:intergenic 15.0%
!!! TTTTTTAACCTTTATCATAA+GGG - chr4.1:51046488-51046507 MS.gene51353:intron 15.0%
!! ACAAATCAATGGAAAATAAA+AGG - chr4.1:51046329-51046348 MS.gene51353:intron 20.0%
!! GTAATGAAGATAAGAAATAT+TGG - chr4.1:51047244-51047263 MS.gene51353:intron 20.0%
!! TAAGGTATGAAAAATAACTA+GGG + chr4.1:51044521-51044540 None:intergenic 20.0%
!! TTAAGGTATGAAAAATAACT+AGG + chr4.1:51044522-51044541 None:intergenic 20.0%
!! TTCTCAAATATACTAAAGAA+TGG + chr4.1:51044889-51044908 None:intergenic 20.0%
!!! AAATTTTGCAGAAATTACAA+AGG + chr4.1:51046435-51046454 None:intergenic 20.0%
!!! ATTTAGGCTTTCTAAAATTT+AGG - chr4.1:51046800-51046819 MS.gene51353:intron 20.0%
!!! ATTTTGTTGTATAGTCTTAA+TGG + chr4.1:51044952-51044971 None:intergenic 20.0%
!!! GAACTGTATATTATTGATTA+GGG + chr4.1:51045160-51045179 None:intergenic 20.0%
!!! GAGTTTGATTTTGAATTTTT+AGG - chr4.1:51045514-51045533 MS.gene51353:intron 20.0%
!!! GTAATTTCTGCAAAATTTTT+TGG - chr4.1:51046437-51046456 MS.gene51353:intron 20.0%
!!! TAACCTTAGCAATTTAATTT+AGG - chr4.1:51046784-51046803 MS.gene51353:intron 20.0%
!!! TATGCTTTATTTTTCTCTAA+TGG - chr4.1:51046249-51046268 MS.gene51353:intron 20.0%
!!! TCATTGATGACTAAATTAAA+GGG + chr4.1:51045118-51045137 None:intergenic 20.0%
!!! TGAACTGTATATTATTGATT+AGG + chr4.1:51045161-51045180 None:intergenic 20.0%
!!! TTCATTGATGACTAAATTAA+AGG + chr4.1:51045119-51045138 None:intergenic 20.0%
! AAGCCTAAATTAAATTGCTA+AGG + chr4.1:51046790-51046809 None:intergenic 25.0%
! ATATAAGAATGGGAATAATG+AGG - chr4.1:51046179-51046198 MS.gene51353:CDS 25.0%
! ATGAAGATAAGAAATATTGG+TGG - chr4.1:51047247-51047266 MS.gene51353:intron 25.0%
! CTGTCATATAATAATAACTG+TGG + chr4.1:51045246-51045265 None:intergenic 25.0%
! GTTTATGATTATTGTAGTGA+TGG - chr4.1:51047342-51047361 MS.gene51353:CDS 25.0%
! TATTCTGCAATTAACCAATT+AGG + chr4.1:51046387-51046406 None:intergenic 25.0%
! TCTCTGTACCAAAAAAAAAA+TGG + chr4.1:51044919-51044938 None:intergenic 25.0%
! TGAAGATAAGAAATATTGGT+GGG - chr4.1:51047248-51047267 MS.gene51353:intron 25.0%
! TGTATGTGTATGAATTATTG+AGG - chr4.1:51045411-51045430 MS.gene51353:intron 25.0%
! TTTATGTCAATACTCCTAAT+TGG - chr4.1:51046370-51046389 MS.gene51353:intron 25.0%
!! ATGAAAAATAACTAGGGTTT+TGG + chr4.1:51044515-51044534 None:intergenic 25.0%
!! ATTGATAACAAAGGCTTTAT+AGG + chr4.1:51047169-51047188 None:intergenic 25.0%
!!! AAATTAAGCACTGACAAATT+AGG + chr4.1:51047279-51047298 None:intergenic 25.0%
!!! TTCAACAATCAGATTTTTAG+TGG - chr4.1:51046981-51047000 MS.gene51353:CDS 25.0%
!!! TTTTTGACTCATTTGATGTT+TGG + chr4.1:51045323-51045342 None:intergenic 25.0%
!!! TTTTTTTACTGTTGATTGAG+TGG + chr4.1:51046515-51046534 None:intergenic 25.0%
AATATGGAATGCAGATGATT+GGG - chr4.1:51047089-51047108 MS.gene51353:intron 30.0%
AATGTGTATGTGAATGGAAT+TGG - chr4.1:51045571-51045590 MS.gene51353:intron 30.0%
ACATTTGTTTGCACAGAATA+AGG + chr4.1:51045556-51045575 None:intergenic 30.0%
ATCAAAGTCATCAACTTATG+TGG - chr4.1:51047301-51047320 MS.gene51353:CDS 30.0%
ATTATTGTAGTGATGGTTCT+AGG - chr4.1:51047349-51047368 MS.gene51353:CDS 30.0%
ATTCAAAATCAAACTCGTTG+TGG + chr4.1:51045510-51045529 None:intergenic 30.0%
ATTGAACTATATACTCCCAT+TGG + chr4.1:51047072-51047091 None:intergenic 30.0%
ATTTGATGTTTGGAAAGTAG+GGG + chr4.1:51045313-51045332 None:intergenic 30.0%
CAAACAAATGTGTATGTGAA+TGG - chr4.1:51045565-51045584 MS.gene51353:intron 30.0%
CAATGGAAAATAAAAGGTGA+TGG - chr4.1:51046335-51046354 MS.gene51353:intron 30.0%
CATTTGATGTTTGGAAAGTA+GGG + chr4.1:51045314-51045333 None:intergenic 30.0%
CCAAAAGCAAGTATATGTTT+GGG - chr4.1:51044755-51044774 MS.gene51353:intron 30.0%
GAATTATGTACCCAACTTAT+GGG + chr4.1:51046829-51046848 None:intergenic 30.0%
GCAAATATTAATTGGACAAG+AGG + chr4.1:51045272-51045291 None:intergenic 30.0%
GCTTTATAGGTTGCAATAAA+GGG + chr4.1:51047156-51047175 None:intergenic 30.0%
GTCACTCAAATACAAATCAA+TGG - chr4.1:51046318-51046337 MS.gene51353:intron 30.0%
TCAAAGTCATCAACTTATGT+GGG - chr4.1:51047302-51047321 MS.gene51353:CDS 30.0%
TCATTTGATGTTTGGAAAGT+AGG + chr4.1:51045315-51045334 None:intergenic 30.0%
TCCAAAAGCAAGTATATGTT+TGG - chr4.1:51044754-51044773 MS.gene51353:intron 30.0%
TGAATTATGTACCCAACTTA+TGG + chr4.1:51046830-51046849 None:intergenic 30.0%
TTCTTCACATAGTACTAACA+TGG - chr4.1:51045751-51045770 MS.gene51353:intron 30.0%
TTGGAAGAGTTCATCAAATT+TGG + chr4.1:51044388-51044407 None:intergenic 30.0%
! AGTTGTCAAGTTTGAGTTTA+AGG + chr4.1:51044452-51044471 None:intergenic 30.0%
! ATTAGAGTGAGAGTGTAATA+AGG + chr4.1:51045691-51045710 None:intergenic 30.0%
! TAGGATACTACTAGACTTTT+TGG - chr4.1:51046885-51046904 MS.gene51353:intron 30.0%
! TGGGAGTATATAGTTCAATA+TGG - chr4.1:51047073-51047092 MS.gene51353:intron 30.0%
! TTCACTCTTACTCTATTTTC+TGG - chr4.1:51045641-51045660 MS.gene51353:intron 30.0%
!! AAAATAACTAGGGTTTTGGT+TGG + chr4.1:51044511-51044530 None:intergenic 30.0%
!!! GCTTTATTTTTCTCTAATGG+TGG - chr4.1:51046252-51046271 MS.gene51353:intron 30.0%
AAAGGTTAAGTCTTTGCTCA+CGG + chr4.1:51045600-51045619 None:intergenic 35.0%
AAATCAAACTCGTTGTGGTT+TGG + chr4.1:51045505-51045524 None:intergenic 35.0%
AACAATGACAATGGTCATTC+AGG - chr4.1:51044718-51044737 MS.gene51353:CDS 35.0%
AACTATATACTCCCATTGGT+TGG + chr4.1:51047068-51047087 None:intergenic 35.0%
AAGAATGGGAATAATGAGGT+TGG - chr4.1:51046183-51046202 MS.gene51353:CDS 35.0%
AAGAGTGAAAATCCTTGGTA+GGG + chr4.1:51045630-51045649 None:intergenic 35.0%
ACACACAAACATGGAACATA+AGG - chr4.1:51047023-51047042 MS.gene51353:intron 35.0%
ACACATTCAACAGGAGTAAT+AGG + chr4.1:51047378-51047397 None:intergenic 35.0%
AGAGTAAGAGTGAAAATCCT+TGG + chr4.1:51045635-51045654 None:intergenic 35.0%
AGGTTAAAAAATGTTGCGCA+TGG + chr4.1:51046479-51046498 None:intergenic 35.0%
AGTGGATGAAACACCAATAA+GGG - chr4.1:51046999-51047018 MS.gene51353:intron 35.0%
ATGAATGCCACGTTAAGAAA+AGG + chr4.1:51044575-51044594 None:intergenic 35.0%
ATGACAATCAAAGGCTATGA+TGG + chr4.1:51046283-51046302 None:intergenic 35.0%
ATGTTTGTGTGTACCCTTAT+TGG + chr4.1:51047015-51047034 None:intergenic 35.0%
ATTTGTATTTGAGTGACCCA+CGG + chr4.1:51046315-51046334 None:intergenic 35.0%
CAATATGGAATGCAGATGAT+TGG - chr4.1:51047088-51047107 MS.gene51353:intron 35.0%
CACACAAACATGGAACATAA+GGG - chr4.1:51047024-51047043 MS.gene51353:intron 35.0%
CTGAATACCAAATGTCCTAT+GGG - chr4.1:51046654-51046673 MS.gene51353:intron 35.0%
CTGCAATGGATAATACAAAG+AGG - chr4.1:51047214-51047233 MS.gene51353:intron 35.0%
GAGTCTGAAAAGGTTGTAAA+AGG - chr4.1:51046208-51046227 MS.gene51353:CDS 35.0%
GAGTTCATCAAATTTGGAAG+AGG + chr4.1:51044382-51044401 None:intergenic 35.0%
GTGATATAGATGTCATCAGA+GGG - chr4.1:51045478-51045497 MS.gene51353:intron 35.0%
TAAGAGTGAAAATCCTTGGT+AGG + chr4.1:51045631-51045650 None:intergenic 35.0%
TCTGAATACCAAATGTCCTA+TGG - chr4.1:51046653-51046672 MS.gene51353:intron 35.0%
TGAATGCCACGTTAAGAAAA+GGG + chr4.1:51044574-51044593 None:intergenic 35.0%
TGTGATATAGATGTCATCAG+AGG - chr4.1:51045477-51045496 MS.gene51353:intron 35.0%
! AATAAGGGTACACACAAACA+TGG - chr4.1:51047014-51047033 MS.gene51353:intron 35.0%
! AATTTAGGATGCCCATAAGT+TGG - chr4.1:51046815-51046834 MS.gene51353:intron 35.0%
! AGTATTGGGGCAAGAAAATA+AGG + chr4.1:51046694-51046713 None:intergenic 35.0%
! ATTATTGATTAGGGTCCAAG+AGG + chr4.1:51045151-51045170 None:intergenic 35.0%
! ATTTAGGATGCCCATAAGTT+GGG - chr4.1:51046816-51046835 MS.gene51353:intron 35.0%
! CCCAAACATATACTTGCTTT+TGG + chr4.1:51044758-51044777 None:intergenic 35.0%
! GGCTTTATAGGTTGCAATAA+AGG + chr4.1:51047157-51047176 None:intergenic 35.0%
! TAAGGGTATACCATATCCAA+AGG - chr4.1:51047041-51047060 MS.gene51353:intron 35.0%
! TAGTGGATGAAACACCAATA+AGG - chr4.1:51046998-51047017 MS.gene51353:intron 35.0%
! TTTGACCATTTTCTCCATGA+AGG - chr4.1:51044420-51044439 MS.gene51353:CDS 35.0%
!! CTCACAAGCATTGATAACAA+AGG + chr4.1:51047178-51047197 None:intergenic 35.0%
!! GAATTTTTAGGCAACACAAC+AGG - chr4.1:51045526-51045545 MS.gene51353:intron 35.0%
!! TTGTTTCTAACATGTGTGCT+TGG - chr4.1:51046575-51046594 MS.gene51353:intron 35.0%
AAATGGTCAAAAGCCCAACT+TGG + chr4.1:51044411-51044430 None:intergenic 40.0%
AATGAAGATGCTCTACCTCT+TGG - chr4.1:51045133-51045152 MS.gene51353:intron 40.0%
ACATGTTCCCATAGGACATT+TGG + chr4.1:51046664-51046683 None:intergenic 40.0%
ATATCCAAAGGACCAACCAA+TGG - chr4.1:51047053-51047072 MS.gene51353:intron 40.0%
ATGAACTCTTCCAACCAAGT+TGG - chr4.1:51044394-51044413 MS.gene51353:CDS 40.0%
CTCAAACTTGACAACTCTTC+TGG - chr4.1:51044456-51044475 MS.gene51353:CDS 40.0%
GAGGAAGAGTGAAAACAGAT+TGG - chr4.1:51047121-51047140 MS.gene51353:intron 40.0%
GATCTCCTTCATGGAGAAAA+TGG + chr4.1:51044428-51044447 None:intergenic 40.0%
GCAAACTAACATGTTCCCAT+AGG + chr4.1:51046672-51046691 None:intergenic 40.0%
GGGAACACAATGACAATCAA+AGG + chr4.1:51046292-51046311 None:intergenic 40.0%
GTCATCAACTTATGTGGGTT+AGG - chr4.1:51047307-51047326 MS.gene51353:CDS 40.0%
GTGAGCAAAGACTTAACCTT+TGG - chr4.1:51045599-51045618 MS.gene51353:intron 40.0%
GTTTAAGGAGATCTCCTTCA+TGG + chr4.1:51044437-51044456 None:intergenic 40.0%
TATCCAAAGGACCAACCAAT+GGG - chr4.1:51047054-51047073 MS.gene51353:intron 40.0%
TGAACTCTTCCAACCAAGTT+GGG - chr4.1:51044395-51044414 MS.gene51353:CDS 40.0%
TGAGTGGTCCCTTATGATAA+AGG + chr4.1:51046499-51046518 None:intergenic 40.0%
TTTGTATTTGAGTGACCCAC+GGG + chr4.1:51046314-51046333 None:intergenic 40.0%
! ATTGGTTGGTCCTTTGGATA+TGG + chr4.1:51047054-51047073 None:intergenic 40.0%
! TCAAGACCCTTTTCTTAACG+TGG - chr4.1:51044565-51044584 MS.gene51353:CDS 40.0%
!! ATCCAGCTTAAGCTTGTTGA+AGG - chr4.1:51044787-51044806 MS.gene51353:intron 40.0%
!! GTGGGTTAGGAATCATCATA+TGG - chr4.1:51047320-51047339 MS.gene51353:CDS 40.0%
CACCTTCAACAAGCTTAAGC+TGG + chr4.1:51044792-51044811 None:intergenic 45.0%
GAGGTTGGATGAGTCTGAAA+AGG - chr4.1:51046198-51046217 MS.gene51353:CDS 45.0%
TTGTATTTGAGTGACCCACG+GGG + chr4.1:51046313-51046332 None:intergenic 45.0%
TTGTGAGTGTCCAGTTTCAG+TGG - chr4.1:51047191-51047210 MS.gene51353:intron 45.0%
!! CGATGATGGACACATTCAAC+AGG + chr4.1:51047387-51047406 None:intergenic 45.0%
!!! AATTTTTTACATTTATATTT+TGG - chr4.1:51045194-51045213 MS.gene51353:intron 5.0%
ATTGTCATTGTGTTCCCCCG+TGG - chr4.1:51046295-51046314 MS.gene51353:intron 50.0%
CCTTGGTAGGGTCAAACCAA+AGG + chr4.1:51045618-51045637 None:intergenic 50.0%
CTTGTTGAAGGTGACTCTGC+TGG - chr4.1:51044799-51044818 MS.gene51353:intron 50.0%
GACAATGGTCATTCAGGTGC+TGG - chr4.1:51044724-51044743 MS.gene51353:CDS 50.0%
GATGATTGGGCAACACAAGG+AGG - chr4.1:51047102-51047121 MS.gene51353:intron 50.0%
GCAGATGATTGGGCAACACA+AGG - chr4.1:51047099-51047118 MS.gene51353:intron 50.0%
GGTCAAAAGCCCAACTTGGT+TGG + chr4.1:51044407-51044426 None:intergenic 50.0%
TCCAGTTTCAGTGGCTGCAA+TGG - chr4.1:51047200-51047219 MS.gene51353:intron 50.0%
TCCATTGCAGCCACTGAAAC+TGG + chr4.1:51047204-51047223 None:intergenic 50.0%
TGTATTTGAGTGACCCACGG+GGG + chr4.1:51046312-51046331 None:intergenic 50.0%
TTGTCATTGTGTTCCCCCGT+GGG - chr4.1:51046296-51046315 MS.gene51353:intron 50.0%
! ACTCCCATTGGTTGGTCCTT+TGG + chr4.1:51047060-51047079 None:intergenic 50.0%
!! CCTTTGGTTTGACCCTACCA+AGG - chr4.1:51045615-51045634 MS.gene51353:intron 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 51044324 51047416 51044324 ID=MS.gene51353
chr4.1 mRNA 51044324 51047416 51044324 ID=MS.gene51353.t1;Parent=MS.gene51353
chr4.1 exon 51047263 51047416 51047263 ID=MS.gene51353.t1.exon1;Parent=MS.gene51353.t1
chr4.1 CDS 51047263 51047416 51047263 ID=cds.MS.gene51353.t1;Parent=MS.gene51353.t1
chr4.1 exon 51046900 51047000 51046900 ID=MS.gene51353.t1.exon2;Parent=MS.gene51353.t1
chr4.1 CDS 51046900 51047000 51046900 ID=cds.MS.gene51353.t1;Parent=MS.gene51353.t1
chr4.1 exon 51046060 51046253 51046060 ID=MS.gene51353.t1.exon3;Parent=MS.gene51353.t1
chr4.1 CDS 51046060 51046253 51046060 ID=cds.MS.gene51353.t1;Parent=MS.gene51353.t1
chr4.1 exon 51044324 51044747 51044324 ID=MS.gene51353.t1.exon4;Parent=MS.gene51353.t1
chr4.1 CDS 51044324 51044747 51044324 ID=cds.MS.gene51353.t1;Parent=MS.gene51353.t1
Gene Sequence

>MS.gene51353

ATGTCTTGGTTTTTTGGTTTGTTTGTGTGTCTTATGTTAAGTGTGTCTTTGTCTACCTCTTCCAAATTTGATGAACTCTTCCAACCAAGTTGGGCTTTTGACCATTTTCTCCATGAAGGAGATCTCCTTAAACTCAAACTTGACAACTCTTCTGGTAAATACATCATTTCTTTGTAAACTTTTTCCAACCAAAACCCTAGTTATTTTTCATACCTTAAAATCTTTTTAAACTAAGAATCTATCAAGACCCTTTTCTTAACGTGGCATTCATTTAATCCATATTTTAAACATATTTTTGTAAATTATTTTGATCACTTATAAAATGGAATTAATTAATATGAGAGTGTTTCTGTTTATTTAATAAAAAAATTGCTAGTTATATGTGTAATTAATTAACAATGACAATGGTCATTCAGGTGCTGGTTTTGTATCCAAAAGCAAGTATATGTTTGGGAGAGTATCTATCCAGCTTAAGCTTGTTGAAGGTGACTCTGCTGGAACTGTTACTGCTTTCTATGTAAGTGACTTAATTAAATATAAACTAAAATCAATTATTTTATTACCATTCTTTAGTATATTTGAGAAAAAAAGTCCATTTTTTTTTTGGTACAGAGAAAAAAAAAGTCCATTAAGACTATACAACAAAATACACTCTTATTTTTATTATTATTTATTTTAATCTAATGTTAAGGAAGATCAAGAATAAATTTGAACTTTGAAGTGATGTGAAAAAAAAAATATCAATTCAAAATACATACTATTTGATGTGTCTATCTTTGATTTACTATTCACCCTTTAATTTAGTCATCAATGAAGATGCTCTACCTCTTGGACCCTAATCAATAATATACAGTTCATTCAAGTGCAAGAAATTTTTTACATTTATATTTTGGTACTCAATGAAAAGACTACTATAGTACCACAGTTATTATTATATGACAGCTACCTCTTGTCCAATTAATATTTGCTTTAGTAACTTTTTTTTTCCCCTACTTTCCAAACATCAAATGAGTCAAAAAACAAAGTACAAATTATCAATACTTTATATTCATATTATTCTGTGGTTGAATTGACATTGTTACTTTAATGTATGTGTATGAATTATTGAGGAATCTAATTGACTATGATCTGACATTTGTGATGATAAATAATTTGTGATATAGATGTCATCAGAGGGTCCAAACCACAACGAGTTTGATTTTGAATTTTTAGGCAACACAACAGGAGAACCTTATTCTGTGCAAACAAATGTGTATGTGAATGGAATTGGTAACCGTGAGCAAAGACTTAACCTTTGGTTTGACCCTACCAAGGATTTTCACTCTTACTCTATTTTCTGGAATCAACGTCAAGTTGTGTAAGCACCTTATTACACTCTCACTCTAATAATATTATGTGTAACATTATTTATTTTATGCTAAATTTTGTTCTTCACATAGTACTAACATGGTGCATTACTTGCAAATTTTTATATTTCTTAATACATTTATAACATATATTTAGCACATTTATAACATAATTAGCAAACAATTGTTAATAAAGTTAAGTGAAATAACTTTAGTTAACTTATTACATATAGTTAGCATATTTATAACATAGTTAACTTGTAAATTTTTCACACTTTTTATTTTTTTTAAAAAGTATGAAATAACTTTATATGATACTCAATAGTAATATTTTTTTTCCTTGTCAAAAAGTAATATTTTTTTTAATGAAAGAAATAAGTAGTAATTATTATGTTTTCTACTTTAGTATTATATATGTAAATTTAGTGAAGTAATGAAGTTTAATAACAAAAGTGAAGAGAAAGTGAAGAGAATATTTTTCATAAAAAAAGAAAGGTGAAGAGAATAAAATATAAGAATGGGAATAATGAGGTTGGATGAGTCTGAAAAGGTTGTAAAAGGAAACAAAAAAAAAATGCATATGCTTTATTTTTCTCTAATGGTGGTTGAGCCATCATAGCCTTTGATTGTCATTGTGTTCCCCCGTGGGTCACTCAAATACAAATCAATGGAAAATAAAAGGTGATGGAATCTTGTACAATTTATGTCAATACTCCTAATTGGTTAATTGCAGAATACGTTTGTACAGACACTAAACTATGTCCTTTGTAATTTCTGCAAAATTTTTTGGACATTTTAGCAGCATGCCATGCGCAACATTTTTTAACCTTTATCATAAGGGACCACTCAATCAACAGTAAAAAAAATTAAAAGAAAATATGTCCATTAGTAAAAATATTTTTTGATTGTTTCTAACATGTGTGCTTGGACTTAACTAGAATAATTTTGTTCTTGAAAGCAATAATTTTTAAAATTTTCAAGAATCTGAATACCAAATGTCCTATGGGAACATGTTAGTTTGCCTTATTTTCTTGCCCCAATACTTTAATATATTCAATGATTGGTCATTTTTAATTTAGTCTATTCCTAAATTGTAATATTTTTTCATTTTAATTAACCTTAGCAATTTAATTTAGGCTTTCTAAAATTTAGGATGCCCATAAGTTGGGTACATAATTCACACTATGTTCACTAATCAATATTAATTCAATTAATTAGGATACTACTAGACTTTTTGGTATTATTATTTTTTTATATATTTTTTTATTTATAATTATTGATGAAAATTCTAACTTTTTATTTTTTTTATTTTTCAACAATCAGATTTTTAGTGGATGAAACACCAATAAGGGTACACACAAACATGGAACATAAGGGTATACCATATCCAAAGGACCAACCAATGGGAGTATATAGTTCAATATGGAATGCAGATGATTGGGCAACACAAGGAGGAAGAGTGAAAACAGATTGGTCTCATGCACCCTTTATTGCAACCTATAAAGCCTTTGTTATCAATGCTTGTGAGTGTCCAGTTTCAGTGGCTGCAATGGATAATACAAAGAGGTGTAGTAGTAATGAAGATAAGAAATATTGGTGGGATGAGCCTAATTTGTCAGTGCTTAATTTGCATCAAAGTCATCAACTTATGTGGGTTAGGAATCATCATATGGTTTATGATTATTGTAGTGATGGTTCTAGGTTTCCTATTACTCCTGTTGAATGTGTCCATCATCGTCATAGCTAG
Protein sequence

>MS.gene51353.t1

MSWFFGLFVCLMLSVSLSTSSKFDELFQPSWAFDHFLHEGDLLKLKLDNSSGAGFVSKSKYMFGRVSIQLKLVEGDSAGTVTAFYMSSEGPNHNEFDFEFLGNTTGEPYSVQTNVYVNGIGNREQRLNLWFDPTKDFHSYSIFWNQRQVVFLVDETPIRVHTNMEHKGIPYPKDQPMGVYSSIWNADDWATQGGRVKTDWSHAPFIATYKAFVINACECPVSVAAMDNTKRCSSNEDKKYWWDEPNLSVLNLHQSHQLMWVRNHHMVYDYCSDGSRFPITPVECVHHRHS