Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51481.t1 | KEH24706.1 | 89.3 | 178 | 19 | 0 | 1 | 178 | 73 | 250 | 8.90E-81 | 309.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51481.t1 | Q54CI5 | 32.5 | 154 | 85 | 3 | 10 | 148 | 14 | 163 | 2.0e-09 | 63.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51481.t1 | A0A072U5R7 | 89.3 | 178 | 19 | 0 | 1 | 178 | 73 | 250 | 6.4e-81 | 309.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene83474 | MS.gene51481 | PPI |
| MS.gene51481 | MS.gene028111 | PPI |
| MS.gene51481 | MS.gene72809 | PPI |
| MS.gene82121 | MS.gene51481 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51481.t1 | MTR_6g003930 | 89.326 | 178 | 19 | 0 | 1 | 178 | 1 | 178 | 1.22e-113 | 320 |
| MS.gene51481.t1 | MTR_3g006810 | 89.326 | 178 | 19 | 0 | 1 | 178 | 1 | 178 | 4.40e-113 | 319 |
| MS.gene51481.t1 | MTR_6g003930 | 89.326 | 178 | 19 | 0 | 1 | 178 | 73 | 250 | 4.97e-112 | 319 |
| MS.gene51481.t1 | MTR_4g074580 | 78.947 | 133 | 27 | 1 | 1 | 132 | 1 | 133 | 1.03e-69 | 207 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51481.t1 | AT4G10130 | 47.929 | 169 | 81 | 2 | 1 | 169 | 1 | 162 | 4.72e-52 | 164 |
| MS.gene51481.t1 | AT4G10130 | 47.929 | 169 | 81 | 2 | 1 | 169 | 1 | 162 | 4.72e-52 | 164 |
| MS.gene51481.t1 | AT4G10130 | 47.929 | 169 | 81 | 2 | 1 | 169 | 1 | 162 | 4.72e-52 | 164 |
| MS.gene51481.t1 | AT4G10130 | 47.929 | 169 | 81 | 2 | 1 | 169 | 1 | 162 | 4.72e-52 | 164 |
| MS.gene51481.t1 | AT4G10130 | 47.929 | 169 | 81 | 2 | 1 | 169 | 1 | 162 | 4.72e-52 | 164 |
Find 29 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCGTTGGAATTGCTGAAAA+TGG | 0.258071 | 3.4:+2330629 | MS.gene51481:CDS |
| AGCTTGTTTGATACTCAGTA+TGG | 0.368432 | 3.4:+2330749 | MS.gene51481:CDS |
| AATGTCTTCAATAACTTATC+AGG | 0.370073 | 3.4:-2330363 | None:intergenic |
| AAGAAATTCGTGCTAGTTAC+CGG | 0.375273 | 3.4:+2330319 | MS.gene51481:CDS |
| TTATGATGCTCTCAATGTTA+TGG | 0.375614 | 3.4:+2330281 | MS.gene51481:CDS |
| CTCAATGGCGATACTTTAAC+AGG | 0.376329 | 3.4:+2330690 | MS.gene51481:CDS |
| GGATATTCCTTACTGAGAAA+TGG | 0.383181 | 3.4:+2330651 | MS.gene51481:CDS |
| CTCTCGTTTATTATATGATA+AGG | 0.385288 | 3.4:+2330467 | MS.gene51481:CDS |
| ACTATTTCCATTTCTCAGTA+AGG | 0.412474 | 3.4:-2330658 | None:intergenic |
| TTCTCAAAGTACAAAAGGCC+TGG | 0.421729 | 3.4:+2330424 | MS.gene51481:CDS |
| AAATCTTCTGCAACTTCAGC+AGG | 0.459358 | 3.4:-2330519 | None:intergenic |
| GAGATTTCTCAAAGTACAAA+AGG | 0.460367 | 3.4:+2330419 | MS.gene51481:CDS |
| TCGTTGGAATTGCTGAAAAT+GGG | 0.480267 | 3.4:+2330630 | MS.gene51481:CDS |
| TGAAGTTCTTTGATTACTGT+CGG | 0.490895 | 3.4:-2330394 | None:intergenic |
| CAGCAATTCCAACGAGTCGA+CGG | 0.501247 | 3.4:-2330622 | None:intergenic |
| TTCTGCAACTTCAGCAGGCA+AGG | 0.501366 | 3.4:-2330514 | None:intergenic |
| TCTCAAAGTACAAAAGGCCT+GGG | 0.506541 | 3.4:+2330425 | MS.gene51481:CDS |
| TTACTTCTCCGTCGACTCGT+TGG | 0.525212 | 3.4:+2330614 | MS.gene51481:CDS |
| TCTTGACAAGCATGACATTG+AGG | 0.539456 | 3.4:+2330251 | MS.gene51481:CDS |
| AATAGTATATCTATACTCAA+TGG | 0.541429 | 3.4:+2330675 | MS.gene51481:CDS |
| ATCAGGATGTAAACTGAGTA+CGG | 0.541767 | 3.4:-2330346 | None:intergenic |
| TGAATCGCTTAGAATTTCCC+AGG | 0.557635 | 3.4:-2330442 | None:intergenic |
| ATAAGGAGCTGCAAATATCA+AGG | 0.567304 | 3.4:+2330484 | MS.gene51481:CDS |
| GGAGCTGCAAATATCAAGGC+GGG | 0.573951 | 3.4:+2330488 | MS.gene51481:CDS |
| GGATCCGCGATTCTTCCTTG+TGG | 0.604645 | 3.4:+2330711 | MS.gene51481:CDS |
| GTAAACTGAGTACGGCACTC+CGG | 0.642448 | 3.4:-2330338 | None:intergenic |
| AGATCCACAAGGAAGAATCG+CGG | 0.707890 | 3.4:-2330715 | None:intergenic |
| AGGAGCTGCAAATATCAAGG+CGG | 0.728242 | 3.4:+2330487 | MS.gene51481:CDS |
| TTTAACAAACAAGATCCACA+AGG | 0.735285 | 3.4:-2330726 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAGTATATCTATACTCAA+TGG | + | chr3.4:2330675-2330694 | MS.gene51481:CDS | 20.0% |
| ! | AATGTCTTCAATAACTTATC+AGG | - | chr3.4:2330366-2330385 | None:intergenic | 25.0% |
| ! | CTCTCGTTTATTATATGATA+AGG | + | chr3.4:2330467-2330486 | MS.gene51481:CDS | 25.0% |
| ACTATTTCCATTTCTCAGTA+AGG | - | chr3.4:2330661-2330680 | None:intergenic | 30.0% | |
| GAGATTTCTCAAAGTACAAA+AGG | + | chr3.4:2330419-2330438 | MS.gene51481:CDS | 30.0% | |
| TGAAGTTCTTTGATTACTGT+CGG | - | chr3.4:2330397-2330416 | None:intergenic | 30.0% | |
| TTATGATGCTCTCAATGTTA+TGG | + | chr3.4:2330281-2330300 | MS.gene51481:CDS | 30.0% | |
| TTTAACAAACAAGATCCACA+AGG | - | chr3.4:2330729-2330748 | None:intergenic | 30.0% | |
| ! | CTATTTTATCAATGTCGATG+TGG | + | chr3.4:2330588-2330607 | MS.gene51481:CDS | 30.0% |
| AAGAAATTCGTGCTAGTTAC+CGG | + | chr3.4:2330319-2330338 | MS.gene51481:CDS | 35.0% | |
| ATAAGGAGCTGCAAATATCA+AGG | + | chr3.4:2330484-2330503 | MS.gene51481:CDS | 35.0% | |
| ATCAGGATGTAAACTGAGTA+CGG | - | chr3.4:2330349-2330368 | None:intergenic | 35.0% | |
| GGATATTCCTTACTGAGAAA+TGG | + | chr3.4:2330651-2330670 | MS.gene51481:CDS | 35.0% | |
| TCGTTGGAATTGCTGAAAAT+GGG | + | chr3.4:2330630-2330649 | MS.gene51481:CDS | 35.0% | |
| ! | AGCTTGTTTGATACTCAGTA+TGG | + | chr3.4:2330749-2330768 | MS.gene51481:CDS | 35.0% |
| AAATCTTCTGCAACTTCAGC+AGG | - | chr3.4:2330522-2330541 | None:intergenic | 40.0% | |
| CTCAATGGCGATACTTTAAC+AGG | + | chr3.4:2330690-2330709 | MS.gene51481:CDS | 40.0% | |
| CTCGTTGGAATTGCTGAAAA+TGG | + | chr3.4:2330629-2330648 | MS.gene51481:CDS | 40.0% | |
| TCTCAAAGTACAAAAGGCCT+GGG | + | chr3.4:2330425-2330444 | MS.gene51481:CDS | 40.0% | |
| TCTTGACAAGCATGACATTG+AGG | + | chr3.4:2330251-2330270 | MS.gene51481:CDS | 40.0% | |
| TGAATCGCTTAGAATTTCCC+AGG | - | chr3.4:2330445-2330464 | None:intergenic | 40.0% | |
| TTCTCAAAGTACAAAAGGCC+TGG | + | chr3.4:2330424-2330443 | MS.gene51481:CDS | 40.0% | |
| AGATCCACAAGGAAGAATCG+CGG | - | chr3.4:2330718-2330737 | None:intergenic | 45.0% | |
| AGGAGCTGCAAATATCAAGG+CGG | + | chr3.4:2330487-2330506 | MS.gene51481:CDS | 45.0% | |
| CAGCAATTCCAACGAGTCGA+CGG | - | chr3.4:2330625-2330644 | None:intergenic | 50.0% | |
| GGAGCTGCAAATATCAAGGC+GGG | + | chr3.4:2330488-2330507 | MS.gene51481:CDS | 50.0% | |
| GTAAACTGAGTACGGCACTC+CGG | - | chr3.4:2330341-2330360 | None:intergenic | 50.0% | |
| TTCTGCAACTTCAGCAGGCA+AGG | - | chr3.4:2330517-2330536 | None:intergenic | 50.0% | |
| !! | TTACTTCTCCGTCGACTCGT+TGG | + | chr3.4:2330614-2330633 | MS.gene51481:CDS | 50.0% |
| GGATCCGCGATTCTTCCTTG+TGG | + | chr3.4:2330711-2330730 | MS.gene51481:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 2330246 | 2330782 | 2330246 | ID=MS.gene51481 |
| chr3.4 | mRNA | 2330246 | 2330782 | 2330246 | ID=MS.gene51481.t1;Parent=MS.gene51481 |
| chr3.4 | exon | 2330246 | 2330782 | 2330246 | ID=MS.gene51481.t1.exon1;Parent=MS.gene51481.t1 |
| chr3.4 | CDS | 2330246 | 2330782 | 2330246 | ID=cds.MS.gene51481.t1;Parent=MS.gene51481.t1 |
| Gene Sequence |
| Protein sequence |
