Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51546.t1 | XP_024631053.1 | 97.6 | 85 | 0 | 2 | 1 | 85 | 276 | 358 | 2.50E-33 | 151 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51546.t1 | O81716 | 71.9 | 57 | 16 | 0 | 1 | 57 | 276 | 332 | 1.5e-18 | 93.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51546.t1 | G7I891 | 97.6 | 85 | 0 | 2 | 1 | 85 | 276 | 358 | 1.8e-33 | 151.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050303 | MS.gene51546 | 0.803623 | 3.19E-49 | -1.69E-46 |
| MS.gene051064 | MS.gene51546 | 0.801897 | 7.25E-49 | -1.69E-46 |
| MS.gene058498 | MS.gene51546 | 0.957958 | 7.67E-116 | -1.69E-46 |
| MS.gene058500 | MS.gene51546 | 0.915466 | 5.69E-85 | -1.69E-46 |
| MS.gene058502 | MS.gene51546 | 0.841262 | 5.29E-58 | -1.69E-46 |
| MS.gene060879 | MS.gene51546 | 0.805431 | 1.34E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACCAATTGTTGGCTTGAT+AGG | 0.213993 | 1.4:+7870096 | None:intergenic |
| CCGGTGTTTGGCGCCATCTT+TGG | 0.215436 | 1.4:-7869113 | MS.gene51546:CDS |
| CGATAACATGACAATGATAT+TGG | 0.399156 | 1.4:-7869074 | MS.gene51546:CDS |
| CTTCAACTGTTCTCCAATGT+TGG | 0.431839 | 1.4:+7868968 | None:intergenic |
| GTACAAAATCTACCAATTGT+TGG | 0.434248 | 1.4:+7870087 | None:intergenic |
| CCATCTTTGGCTGTTGGTGA+TGG | 0.439337 | 1.4:-7869100 | MS.gene51546:CDS |
| TTGCCTATCAAGCCAACAAT+TGG | 0.471715 | 1.4:-7870099 | MS.gene51546:CDS |
| TGTACGTCAAGAACTTCTCT+TGG | 0.476519 | 1.4:-7870069 | MS.gene51546:intron |
| AAGAGTGCTAGACCGGTGTT+TGG | 0.509475 | 1.4:-7869125 | MS.gene51546:CDS |
| GAAGATGATTGTTCTTGAGC+CGG | 0.519349 | 1.4:+7869016 | None:intergenic |
| TGCATCATCTGAACCAACAT+TGG | 0.520445 | 1.4:-7868981 | MS.gene51546:CDS |
| TTGGCGCCATCTTTGGCTGT+TGG | 0.522338 | 1.4:-7869106 | MS.gene51546:CDS |
| ACAGGAAACTAAACTTTCTG+AGG | 0.527738 | 1.4:-7869155 | MS.gene51546:intron |
| ACTGCAGACAAGTGCACCGC+CGG | 0.530448 | 1.4:-7869035 | MS.gene51546:CDS |
| CCAAAGATGGCGCCAAACAC+CGG | 0.530584 | 1.4:+7869113 | None:intergenic |
| GGCGGTGCACTTGTCTGCAG+TGG | 0.535694 | 1.4:+7869037 | None:intergenic |
| CCATCACCAACAGCCAAAGA+TGG | 0.553018 | 1.4:+7869100 | None:intergenic |
| TTTGTGAAAGAGTGCTAGAC+CGG | 0.555494 | 1.4:-7869132 | MS.gene51546:CDS |
| GATGATTGTTCTTGAGCCGG+CGG | 0.663671 | 1.4:+7869019 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTTGAACTTATATAATT+TGG | - | chr1.4:7869140-7869159 | MS.gene51546:CDS | 10.0% |
| !! | ATTACATTGAAATAATAACT+TGG | + | chr1.4:7869848-7869867 | None:intergenic | 15.0% |
| !! | CAAATTGTCATTATTAAATT+TGG | - | chr1.4:7869543-7869562 | MS.gene51546:intron | 15.0% |
| !! | CAAATTTAATAATGACAATT+TGG | + | chr1.4:7869545-7869564 | None:intergenic | 15.0% |
| !! | GTTATTATTTCAATGTAATT+TGG | - | chr1.4:7869849-7869868 | MS.gene51546:intron | 15.0% |
| !! | TTAAAAAAATCAAATTGTGA+AGG | - | chr1.4:7869657-7869676 | MS.gene51546:intron | 15.0% |
| !! | AAATTGAACAAAACTGTTAA+TGG | - | chr1.4:7869356-7869375 | MS.gene51546:intron | 20.0% |
| !! | TATAATAGTATTTGTAGTTC+TGG | + | chr1.4:7869442-7869461 | None:intergenic | 20.0% |
| !!! | CAATTTGATTTTTTTAAGTG+TGG | + | chr1.4:7869654-7869673 | None:intergenic | 20.0% |
| ! | AGGTTAACAAATAAACAGTT+CGG | + | chr1.4:7869510-7869529 | None:intergenic | 25.0% |
| !! | AGTAATGATTCTTTTCAGTT+CGG | + | chr1.4:7869321-7869340 | None:intergenic | 25.0% |
| !!! | TTTTTAAGTGTGGTTTGATT+CGG | + | chr1.4:7869644-7869663 | None:intergenic | 25.0% |
| AACTTGGATCATTATGCTTA+GGG | + | chr1.4:7869832-7869851 | None:intergenic | 30.0% | |
| ATGCTTAGGGAAAAAAAACT+TGG | + | chr1.4:7869819-7869838 | None:intergenic | 30.0% | |
| CGATAACATGACAATGATAT+TGG | - | chr1.4:7869998-7870017 | MS.gene51546:intron | 30.0% | |
| GTACAAAATCTACCAATTGT+TGG | + | chr1.4:7868988-7869007 | None:intergenic | 30.0% | |
| TAACTTGGATCATTATGCTT+AGG | + | chr1.4:7869833-7869852 | None:intergenic | 30.0% | |
| TGAATAACAAGGACTGTTTA+TGG | - | chr1.4:7869271-7869290 | MS.gene51546:intron | 30.0% | |
| TTCATATACAAGACAGTACT+AGG | + | chr1.4:7869117-7869136 | None:intergenic | 30.0% | |
| TTCGGTTTAACAGTTCAATT+TGG | - | chr1.4:7869764-7869783 | MS.gene51546:intron | 30.0% | |
| ! | GTTCACAGTTCTTTTCTTTT+TGG | - | chr1.4:7869736-7869755 | MS.gene51546:intron | 30.0% |
| ! | TTAAATTTGGCTCAAATTGC+TGG | - | chr1.4:7869556-7869575 | MS.gene51546:intron | 30.0% |
| AACAAGGACTGTTTATGGTT+TGG | - | chr1.4:7869276-7869295 | MS.gene51546:intron | 35.0% | |
| ACAGGAAACTAAACTTTCTG+AGG | - | chr1.4:7869917-7869936 | MS.gene51546:intron | 35.0% | |
| CAATTTGGTACACAAAGCAA+AGG | + | chr1.4:7869530-7869549 | None:intergenic | 35.0% | |
| GGTATAAAGGTTCCAATCAT+CGG | - | chr1.4:7869397-7869416 | MS.gene51546:intron | 35.0% | |
| TCGTTCGAAAATGTACAGTT+TGG | + | chr1.4:7869470-7869489 | None:intergenic | 35.0% | |
| TGTGCGTTACTTGAATAACA+AGG | - | chr1.4:7869260-7869279 | MS.gene51546:intron | 35.0% | |
| ! | TGATTCTTTTCAGTTCGGTT+TGG | + | chr1.4:7869316-7869335 | None:intergenic | 35.0% |
| !! | CAGTTCTTTTCTTTTTGGTG+TGG | - | chr1.4:7869741-7869760 | MS.gene51546:intron | 35.0% |
| !! | CGATACACTTTTTATCTCAC+AGG | - | chr1.4:7869899-7869918 | MS.gene51546:intron | 35.0% |
| !! | CTTTTCTTTTTGGTGTGGTT+CGG | - | chr1.4:7869746-7869765 | MS.gene51546:intron | 35.0% |
| CATAACACAGAACCGATGAT+TGG | + | chr1.4:7869412-7869431 | None:intergenic | 40.0% | |
| GAAGATGATTGTTCTTGAGC+CGG | + | chr1.4:7870059-7870078 | None:intergenic | 40.0% | |
| TCTGCGCATTAACTGATGTT+AGG | - | chr1.4:7869603-7869622 | MS.gene51546:intron | 40.0% | |
| TGCATCATCTGAACCAACAT+TGG | - | chr1.4:7870091-7870110 | MS.gene51546:CDS | 40.0% | |
| TGGAACCTTTATACCAGTTC+TGG | + | chr1.4:7869392-7869411 | None:intergenic | 40.0% | |
| TGTACGTCAAGAACTTCTCT+TGG | - | chr1.4:7869003-7869022 | MS.gene51546:CDS | 40.0% | |
| TTGCCTATCAAGCCAACAAT+TGG | - | chr1.4:7868973-7868992 | MS.gene51546:CDS | 40.0% | |
| ! | CCTTTATACCAGTTCTGGTT+CGG | + | chr1.4:7869387-7869406 | None:intergenic | 40.0% |
| !! | CTACCAATTGTTGGCTTGAT+AGG | + | chr1.4:7868979-7868998 | None:intergenic | 40.0% |
| !! | TTTGTGAAAGAGTGCTAGAC+CGG | - | chr1.4:7869940-7869959 | MS.gene51546:intron | 40.0% |
| CCAACATTGGAGAACAGTTG+AGG | - | chr1.4:7870104-7870123 | MS.gene51546:CDS | 45.0% | |
| CCGAACCAGAACTGGTATAA+AGG | - | chr1.4:7869384-7869403 | MS.gene51546:intron | 45.0% | |
| GTTCATGAACTGAACTGAGC+TGG | + | chr1.4:7869715-7869734 | None:intergenic | 45.0% | |
| TGCTGGCCATCACAAGTTAA+AGG | - | chr1.4:7869573-7869592 | MS.gene51546:intron | 45.0% | |
| TTCAAGTAACGCACATCAGC+AGG | + | chr1.4:7869255-7869274 | None:intergenic | 45.0% | |
| !! | CGAGTGCCTTTAACTTGTGA+TGG | + | chr1.4:7869582-7869601 | None:intergenic | 45.0% |
| !! | GTGTGGTTTGATTCGGTCTT+TGG | + | chr1.4:7869637-7869656 | None:intergenic | 45.0% |
| CCATCACCAACAGCCAAAGA+TGG | + | chr1.4:7869975-7869994 | None:intergenic | 50.0% | |
| GATGATTGTTCTTGAGCCGG+CGG | + | chr1.4:7870056-7870075 | None:intergenic | 50.0% | |
| ! | CCATCTTTGGCTGTTGGTGA+TGG | - | chr1.4:7869972-7869991 | MS.gene51546:intron | 50.0% |
| ! | TGGTTTGTCCGAACCAGAAC+TGG | - | chr1.4:7869376-7869395 | MS.gene51546:intron | 50.0% |
| !! | AAGAGTGCTAGACCGGTGTT+TGG | - | chr1.4:7869947-7869966 | MS.gene51546:intron | 50.0% |
| CCAAAGATGGCGCCAAACAC+CGG | + | chr1.4:7869962-7869981 | None:intergenic | 55.0% | |
| TTGGCGCCATCTTTGGCTGT+TGG | - | chr1.4:7869966-7869985 | MS.gene51546:intron | 55.0% | |
| ACTGCAGACAAGTGCACCGC+CGG | - | chr1.4:7870037-7870056 | MS.gene51546:intron | 60.0% | |
| CCGGTGTTTGGCGCCATCTT+TGG | - | chr1.4:7869959-7869978 | MS.gene51546:intron | 60.0% | |
| GGCGGTGCACTTGTCTGCAG+TGG | + | chr1.4:7870038-7870057 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 7868970 | 7870124 | 7868970 | ID=MS.gene51546 |
| chr1.4 | mRNA | 7868970 | 7870124 | 7868970 | ID=MS.gene51546.t1;Parent=MS.gene51546 |
| chr1.4 | exon | 7870070 | 7870124 | 7870070 | ID=MS.gene51546.t1.exon1;Parent=MS.gene51546.t1 |
| chr1.4 | CDS | 7870070 | 7870124 | 7870070 | ID=cds.MS.gene51546.t1;Parent=MS.gene51546.t1 |
| chr1.4 | exon | 7868970 | 7869173 | 7868970 | ID=MS.gene51546.t1.exon2;Parent=MS.gene51546.t1 |
| chr1.4 | CDS | 7868970 | 7869173 | 7868970 | ID=cds.MS.gene51546.t1;Parent=MS.gene51546.t1 |
| Gene Sequence |
| Protein sequence |
