Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51856.t1 | XP_003624050.2 | 94 | 352 | 21 | 0 | 1 | 352 | 1 | 352 | 1.60E-182 | 648.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51856.t1 | Q9LMM6 | 56.3 | 334 | 138 | 2 | 1 | 333 | 1 | 327 | 1.7e-100 | 367.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51856.t1 | G7L6L1 | 94.0 | 352 | 21 | 0 | 1 | 352 | 1 | 352 | 1.1e-182 | 648.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050646 | MS.gene51856 | 0.803997 | 2.67E-49 | -1.69E-46 |
| MS.gene057174 | MS.gene51856 | 0.808915 | 2.45E-50 | -1.69E-46 |
| MS.gene058520 | MS.gene51856 | 0.981806 | 1.71E-153 | -1.69E-46 |
| MS.gene060527 | MS.gene51856 | 0.804695 | 1.91E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51856.t1 | MTR_7g078700 | 94.034 | 352 | 21 | 0 | 1 | 352 | 1 | 352 | 0.0 | 677 |
| MS.gene51856.t1 | MTR_8g028710 | 67.507 | 357 | 110 | 3 | 1 | 351 | 1 | 357 | 1.53e-172 | 484 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51856.t1 | AT2G46080 | 57.637 | 347 | 147 | 0 | 1 | 347 | 1 | 347 | 1.00e-147 | 421 |
| MS.gene51856.t1 | AT1G01550 | 55.917 | 338 | 140 | 3 | 1 | 336 | 1 | 331 | 1.31e-132 | 382 |
| MS.gene51856.t1 | AT1G01550 | 55.917 | 338 | 140 | 3 | 1 | 336 | 1 | 331 | 1.31e-132 | 382 |
| MS.gene51856.t1 | AT4G01360 | 39.818 | 329 | 170 | 7 | 16 | 331 | 10 | 323 | 4.86e-70 | 223 |
| MS.gene51856.t1 | AT3G61500 | 35.870 | 184 | 106 | 4 | 1 | 182 | 2 | 175 | 2.94e-26 | 106 |
| MS.gene51856.t1 | AT3G61500 | 36.723 | 177 | 100 | 4 | 9 | 183 | 10 | 176 | 1.54e-25 | 105 |
Find 0 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCTTGTAAATGAGGAAATT+AGG | + | 50225:3063-3082 | MS.gene51856:CDS | 25.0% |
| ! | TCTTGTAAATGAGGAAATTA+GGG | + | 50225:3064-3083 | MS.gene51856:CDS | 25.0% |
| AACCTGAGAATTGAAAATTG+TGG | + | 50225:2822-2841 | MS.gene51856:CDS | 30.0% | |
| AAGGTTAAAAAGTCAGCAAA+AGG | + | 50225:2885-2904 | MS.gene51856:CDS | 30.0% | |
| AGGTTAAAAAGTCAGCAAAA+GGG | + | 50225:2886-2905 | MS.gene51856:CDS | 30.0% | |
| GAATTATACCCTATTATCCA+AGG | + | 50225:3152-3171 | MS.gene51856:CDS | 30.0% | |
| TCTACAGAATCTTGTAAATG+AGG | + | 50225:3055-3074 | MS.gene51856:CDS | 30.0% | |
| ! | TACAAAGAACTTGATGGATA+TGG | + | 50225:2992-3011 | MS.gene51856:CDS | 30.0% |
| ! | TTCTGTAGACCTTTAAAAGT+TGG | - | 50225:3044-3063 | None:intergenic | 30.0% |
| !! | ATTAGGGTTAGATTTTCAAG+TGG | + | 50225:3080-3099 | MS.gene51856:CDS | 30.0% |
| !!! | TTAGGGTTAGATTTTCAAGT+GGG | + | 50225:3081-3100 | MS.gene51856:CDS | 30.0% |
| !!! | TTTGCTGACTTTTTAACCTT+TGG | - | 50225:2885-2904 | None:intergenic | 30.0% |
| AATGGGAAAAATGATCTAGG+TGG | - | 50225:2363-2382 | None:intergenic | 35.0% | |
| ACTAGATCTTCTTACAAAGG+AGG | + | 50225:3256-3275 | MS.gene51856:CDS | 35.0% | |
| AGGACTAGATCTTCTTACAA+AGG | + | 50225:3253-3272 | MS.gene51856:CDS | 35.0% | |
| ATTCACTGTATTGAACGAGT+TGG | + | 50225:3106-3125 | MS.gene51856:CDS | 35.0% | |
| CCAAATGGGAAAAATGATCT+AGG | - | 50225:2366-2385 | None:intergenic | 35.0% | |
| CTACAACACCTTGGATAATA+GGG | - | 50225:3163-3182 | None:intergenic | 35.0% | |
| GAAATGGATAGATGTGTACT+TGG | + | 50225:2623-2642 | MS.gene51856:CDS | 35.0% | |
| TAAAAAGTCAGCAAAAGGGA+AGG | + | 50225:2890-2909 | MS.gene51856:CDS | 35.0% | |
| TAAGTTATCTCCACAACTGT+TGG | + | 50225:2419-2438 | MS.gene51856:CDS | 35.0% | |
| TCTACAACACCTTGGATAAT+AGG | - | 50225:3164-3183 | None:intergenic | 35.0% | |
| ! | AACAACTTCATCCTTGCTTT+TGG | - | 50225:2499-2518 | None:intergenic | 35.0% |
| ! | TGAAAATTGTGGCAGCATTT+TGG | + | 50225:2833-2852 | MS.gene51856:CDS | 35.0% |
| ! | TGCCACAATTTTCAATTCTC+AGG | - | 50225:2827-2846 | None:intergenic | 35.0% |
| !! | ACAACTTCATCCTTGCTTTT+GGG | - | 50225:2498-2517 | None:intergenic | 35.0% |
| !! | ATGAAGTTGTTAGCTTGTCT+TGG | + | 50225:2508-2527 | MS.gene51856:CDS | 35.0% |
| !! | CCTAGATCATTTTTCCCATT+TGG | + | 50225:2363-2382 | MS.gene51856:CDS | 35.0% |
| !! | GTTTTGATGCAAGCTATCTA+TGG | + | 50225:2912-2931 | MS.gene51856:CDS | 35.0% |
| ACGTGCACTGAAGTAAAAGA+TGG | - | 50225:2716-2735 | None:intergenic | 40.0% | |
| AGATCTTCTTACAAAGGAGG+TGG | + | 50225:3259-3278 | MS.gene51856:CDS | 40.0% | |
| AGGCTCTACAAAGAACTTGA+TGG | + | 50225:2986-3005 | MS.gene51856:CDS | 40.0% | |
| ATCCGGAAAGGATTTCCAAA+TGG | - | 50225:2381-2400 | None:intergenic | 40.0% | |
| CAATGGTATCTACAACACCT+TGG | - | 50225:3172-3191 | None:intergenic | 40.0% | |
| CGCATGTGATTCTGTTTCAA+TGG | - | 50225:3189-3208 | None:intergenic | 40.0% | |
| GAAAAGCTGATCTGATGAGA+AGG | - | 50225:2754-2773 | None:intergenic | 40.0% | |
| GATAAAACTGCCAACAGTTG+TGG | - | 50225:2432-2451 | None:intergenic | 40.0% | |
| GCAACAGAGAATTTCTCTCA+AGG | + | 50225:3233-3252 | MS.gene51856:CDS | 40.0% | |
| TCCCATTTGGAAATCCTTTC+CGG | + | 50225:2376-2395 | MS.gene51856:CDS | 40.0% | |
| TCCGGAAAGGATTTCCAAAT+GGG | - | 50225:2380-2399 | None:intergenic | 40.0% | |
| TGACTGATTGGGATGAGAAA+TGG | + | 50225:2607-2626 | MS.gene51856:CDS | 40.0% | |
| TTTCTCATCCCAATCAGTCA+CGG | - | 50225:2607-2626 | None:intergenic | 40.0% | |
| ! | AGCATTTTGGATAATCTGGC+TGG | + | 50225:2846-2865 | MS.gene51856:CDS | 40.0% |
| ! | CATGTTCTTCACTTGATGGT+TGG | + | 50225:2778-2797 | MS.gene51856:CDS | 40.0% |
| ! | GAACTTGATGGATATGGATG+TGG | + | 50225:2998-3017 | MS.gene51856:CDS | 40.0% |
| ! | GCATTTTGGATAATCTGGCT+GGG | + | 50225:2847-2866 | MS.gene51856:CDS | 40.0% |
| ! | TAGCTTGTCTTGGATGACTT+CGG | + | 50225:2518-2537 | MS.gene51856:CDS | 40.0% |
| ! | TCTCATCAGATCAGCTTTTC+CGG | + | 50225:2754-2773 | MS.gene51856:CDS | 40.0% |
| ! | TCTTGGGCTCCAACTTTTAA+AGG | + | 50225:3032-3051 | MS.gene51856:CDS | 40.0% |
| ! | TGGCAGCATTTTGGATAATC+TGG | + | 50225:2842-2861 | MS.gene51856:CDS | 40.0% |
| !! | GGCAGTTTTATCTGCTTTTG+AGG | + | 50225:2440-2459 | MS.gene51856:CDS | 40.0% |
| !!! | ATCTGCTTTTGAGGCAACTT+TGG | + | 50225:2449-2468 | MS.gene51856:CDS | 40.0% |
| !!! | CTTTTGAGGCAACTTTGGAA+GGG | + | 50225:2454-2473 | MS.gene51856:CDS | 40.0% |
| !!! | GTGTTTGCAACTGCTTTTTC+AGG | + | 50225:2966-2985 | MS.gene51856:CDS | 40.0% |
| !!! | TTTTTCCAAGTGGTCTTGTC+TGG | + | 50225:3287-3306 | MS.gene51856:CDS | 40.0% |
| AAACCTCACTCCCAAAAGCA+AGG | + | 50225:2485-2504 | MS.gene51856:CDS | 45.0% | |
| AAGCTCAAGGTCTGTCATCA+GGG | - | 50225:2583-2602 | None:intergenic | 45.0% | |
| AATGATCTAGGTGGATCCTG+TGG | - | 50225:2354-2373 | None:intergenic | 45.0% | |
| ACTGTCAATGACCACGTCTT+AGG | + | 50225:3347-3366 | MS.gene51856:CDS | 45.0% | |
| ATGTGGCTGATGTGTACTCT+TGG | + | 50225:3015-3034 | MS.gene51856:CDS | 45.0% | |
| CAGTGCACGTTGCACAATTT+AGG | + | 50225:2726-2745 | MS.gene51856:CDS | 45.0% | |
| CATCAAGTGAAGAACATGCC+CGG | - | 50225:2776-2795 | None:intergenic | 45.0% | |
| CTGTCAATGACCACGTCTTA+GGG | + | 50225:3348-3367 | MS.gene51856:CDS | 45.0% | |
| CTTCTTACAAAGGAGGTGGA+TGG | + | 50225:3263-3282 | MS.gene51856:CDS | 45.0% | |
| GCAAGCTATCTATGGAGTGA+AGG | + | 50225:2920-2939 | MS.gene51856:CDS | 45.0% | |
| TGACCTCAGATTAGACAGCA+AGG | - | 50225:3321-3340 | None:intergenic | 45.0% | |
| TGCCTTTAGCTGCTATCATC+CGG | - | 50225:2398-2417 | None:intergenic | 45.0% | |
| TGGGTCATCTGATCTTCCAA+AGG | + | 50225:2866-2885 | MS.gene51856:CDS | 45.0% | |
| TGTCAATGACCACGTCTTAG+GGG | + | 50225:3349-3368 | MS.gene51856:CDS | 45.0% | |
| TGTGGCTGATGTGTACTCTT+GGG | + | 50225:3016-3035 | MS.gene51856:CDS | 45.0% | |
| TTAGCTGCTATCATCCGGAA+AGG | - | 50225:2393-2412 | None:intergenic | 45.0% | |
| TTCCGGATGATAGCAGCTAA+AGG | + | 50225:2393-2412 | MS.gene51856:CDS | 45.0% | |
| TTCTCATCCCAATCAGTCAC+GGG | - | 50225:2606-2625 | None:intergenic | 45.0% | |
| ! | ATGCCTTGCTGTCTAATCTG+AGG | + | 50225:3315-3334 | MS.gene51856:CDS | 45.0% |
| ! | CTCATCAGATCAGCTTTTCC+GGG | + | 50225:2755-2774 | MS.gene51856:CDS | 45.0% |
| ! | GAGTTATCACGTCTGAAGCA+GGG | + | 50225:2690-2709 | MS.gene51856:CDS | 45.0% |
| ! | GTTCTTCACTTGATGGTTGG+AGG | + | 50225:2781-2800 | MS.gene51856:CDS | 45.0% |
| ! | TGAGTTATCACGTCTGAAGC+AGG | + | 50225:2689-2708 | MS.gene51856:CDS | 45.0% |
| ! | TTGCTGTCTAATCTGAGGTC+AGG | + | 50225:3320-3339 | MS.gene51856:CDS | 45.0% |
| !! | AAGCTGTTGATTTGTCTGCG+AGG | + | 50225:3129-3148 | MS.gene51856:CDS | 45.0% |
| !! | AGCTGTTGATTTGTCTGCGA+GGG | + | 50225:3130-3149 | MS.gene51856:CDS | 45.0% |
| !!! | GCTTTTGAGGCAACTTTGGA+AGG | + | 50225:2453-2472 | MS.gene51856:CDS | 45.0% |
| AATCAGTCACGGGAAGCTCA+AGG | - | 50225:2596-2615 | None:intergenic | 50.0% | |
| CGGGCATGTTCTTCACTTGA+TGG | + | 50225:2774-2793 | MS.gene51856:CDS | 50.0% | |
| CTTTGTTACCCCCTAAGACG+TGG | - | 50225:3361-3380 | None:intergenic | 50.0% | |
| GAAGCTCAAGGTCTGTCATC+AGG | - | 50225:2584-2603 | None:intergenic | 50.0% | |
| GATGGTTGGAGGCAGCATAT+TGG | + | 50225:2792-2811 | MS.gene51856:CDS | 50.0% | |
| GCGAAGACTGTTGAGAAGCT+AGG | + | 50225:3206-3225 | MS.gene51856:CDS | 50.0% | |
| GTCAATGACCACGTCTTAGG+GGG | + | 50225:3350-3369 | MS.gene51856:CDS | 50.0% | |
| TGAGCTTCCCGTGACTGATT+GGG | + | 50225:2596-2615 | MS.gene51856:CDS | 50.0% | |
| TTGAGCTTCCCGTGACTGAT+TGG | + | 50225:2595-2614 | MS.gene51856:CDS | 50.0% | |
| TTGGATGACTTCGGCTATGC+AGG | + | 50225:2527-2546 | MS.gene51856:CDS | 50.0% | |
| ! | TTGAGGCAACTTTGGAAGGG+AGG | + | 50225:2457-2476 | MS.gene51856:CDS | 50.0% |
| !! | CATCCTTGCTTTTGGGAGTG+AGG | - | 50225:2491-2510 | None:intergenic | 50.0% |
| !! | GGTGGATGGCTTTTTCCAAG+TGG | + | 50225:3277-3296 | MS.gene51856:CDS | 50.0% |
| CACGACCAGACAAGACCACT+TGG | - | 50225:3295-3314 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50225 | gene | 2342 | 3400 | 2342 | ID=MS.gene51856 |
| 50225 | mRNA | 2342 | 3400 | 2342 | ID=MS.gene51856.t1;Parent=MS.gene51856 |
| 50225 | exon | 2342 | 3400 | 2342 | ID=MS.gene51856.t1.exon1;Parent=MS.gene51856.t1 |
| 50225 | CDS | 2342 | 3400 | 2342 | ID=cds.MS.gene51856.t1;Parent=MS.gene51856.t1 |
| Gene Sequence |
| Protein sequence |
