Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058520.t1 | XP_003624050.2 | 94 | 352 | 21 | 0 | 1 | 352 | 1 | 352 | 1.60E-182 | 648.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058520.t1 | Q9LMM6 | 56.3 | 334 | 138 | 2 | 1 | 333 | 1 | 327 | 1.7e-100 | 367.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058520.t1 | G7L6L1 | 94.0 | 352 | 21 | 0 | 1 | 352 | 1 | 352 | 1.1e-182 | 648.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050646 | MS.gene058520 | 0.805754 | 1.15E-49 | -1.69E-46 |
| MS.gene057174 | MS.gene058520 | 0.800405 | 1.46E-48 | -1.69E-46 |
| MS.gene058520 | MS.gene060527 | 0.802437 | 5.61E-49 | -1.69E-46 |
| MS.gene058520 | MS.gene071918 | 0.820525 | 6.55E-53 | -1.69E-46 |
| MS.gene058520 | MS.gene072153 | 0.80412 | 2.52E-49 | -1.69E-46 |
| MS.gene058520 | MS.gene072689 | 0.806701 | 7.24E-50 | -1.69E-46 |
| MS.gene058520 | MS.gene36571 | 0.806858 | 6.71E-50 | -1.69E-46 |
| MS.gene058520 | MS.gene39253 | 0.800201 | 1.61E-48 | -1.69E-46 |
| MS.gene058520 | MS.gene48188 | 0.80844 | 3.09E-50 | -1.69E-46 |
| MS.gene058520 | MS.gene51856 | 0.981806 | 1.71E-153 | -1.69E-46 |
| MS.gene058520 | MS.gene57360 | 0.816585 | 5.13E-52 | -1.69E-46 |
| MS.gene058520 | MS.gene58134 | 0.801471 | 8.87E-49 | -1.69E-46 |
| MS.gene058520 | MS.gene58627 | 0.811083 | 8.36E-51 | -1.69E-46 |
| MS.gene058520 | MS.gene60092 | 0.812215 | 4.74E-51 | -1.69E-46 |
| MS.gene058520 | MS.gene61644 | 0.828948 | 6.77E-55 | -1.69E-46 |
| MS.gene058520 | MS.gene63930 | 0.81027 | 1.25E-50 | -1.69E-46 |
| MS.gene058520 | MS.gene74902 | 0.814824 | 1.27E-51 | -1.69E-46 |
| MS.gene058520 | MS.gene74904 | 0.80152 | 8.66E-49 | -1.69E-46 |
| MS.gene058520 | MS.gene74932 | 0.816632 | 5.00E-52 | -1.69E-46 |
| MS.gene058520 | MS.gene77356 | 0.830757 | 2.45E-55 | -1.69E-46 |
| MS.gene058520 | MS.gene84186 | 0.804797 | 1.82E-49 | -1.69E-46 |
| MS.gene058520 | MS.gene88658 | 0.828287 | 9.78E-55 | -1.69E-46 |
| MS.gene058520 | MS.gene90604 | 0.807923 | 3.99E-50 | -1.69E-46 |
| MS.gene058520 | MS.gene93728 | 0.813879 | 2.05E-51 | -1.69E-46 |
| MS.gene058520 | MS.gene96191 | 0.811977 | 5.34E-51 | -1.69E-46 |
| MS.gene058520 | MS.gene96992 | 0.800366 | 1.49E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058520.t1 | MTR_7g078700 | 94.034 | 352 | 21 | 0 | 1 | 352 | 1 | 352 | 0.0 | 677 |
| MS.gene058520.t1 | MTR_8g028710 | 67.507 | 357 | 110 | 3 | 1 | 351 | 1 | 357 | 1.53e-172 | 484 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058520.t1 | AT2G46080 | 57.637 | 347 | 147 | 0 | 1 | 347 | 1 | 347 | 1.00e-147 | 421 |
| MS.gene058520.t1 | AT1G01550 | 55.917 | 338 | 140 | 3 | 1 | 336 | 1 | 331 | 1.31e-132 | 382 |
| MS.gene058520.t1 | AT1G01550 | 55.917 | 338 | 140 | 3 | 1 | 336 | 1 | 331 | 1.31e-132 | 382 |
| MS.gene058520.t1 | AT4G01360 | 39.818 | 329 | 170 | 7 | 16 | 331 | 10 | 323 | 4.86e-70 | 223 |
| MS.gene058520.t1 | AT3G61500 | 35.870 | 184 | 106 | 4 | 1 | 182 | 2 | 175 | 2.94e-26 | 106 |
| MS.gene058520.t1 | AT3G61500 | 36.723 | 177 | 100 | 4 | 9 | 183 | 10 | 176 | 1.54e-25 | 105 |
Find 0 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCTTGTAAATGAGGAAATT+AGG | - | 50226:11740-11759 | MS.gene058520:CDS | 25.0% |
| ! | TCTTGTAAATGAGGAAATTA+GGG | - | 50226:11741-11760 | MS.gene058520:CDS | 25.0% |
| AACCTGAGAATTGAAAATTG+TGG | - | 50226:11499-11518 | MS.gene058520:CDS | 30.0% | |
| AAGGTTAAAAAGTCAGCAAA+AGG | - | 50226:11562-11581 | MS.gene058520:CDS | 30.0% | |
| AGGTTAAAAAGTCAGCAAAA+GGG | - | 50226:11563-11582 | MS.gene058520:CDS | 30.0% | |
| GAATTATACCCTATTATCCA+AGG | - | 50226:11829-11848 | MS.gene058520:CDS | 30.0% | |
| TCTACAGAATCTTGTAAATG+AGG | - | 50226:11732-11751 | MS.gene058520:CDS | 30.0% | |
| ! | TACAAAGAACTTGATGGATA+TGG | - | 50226:11669-11688 | MS.gene058520:CDS | 30.0% |
| ! | TTCTGTAGACCTTTAAAAGT+TGG | + | 50226:11721-11740 | MS.gene058520:intergenic | 30.0% |
| !! | ATTAGGGTTAGATTTTCAAG+TGG | - | 50226:11757-11776 | MS.gene058520:CDS | 30.0% |
| !!! | TTAGGGTTAGATTTTCAAGT+GGG | - | 50226:11758-11777 | MS.gene058520:CDS | 30.0% |
| !!! | TTTGCTGACTTTTTAACCTT+TGG | + | 50226:11562-11581 | MS.gene058520:intergenic | 30.0% |
| AATGGGAAAAATGATCTAGG+TGG | + | 50226:11040-11059 | MS.gene058520:intergenic | 35.0% | |
| ACTAGATCTTCTTACAAAGG+AGG | - | 50226:11933-11952 | MS.gene058520:CDS | 35.0% | |
| AGGACTAGATCTTCTTACAA+AGG | - | 50226:11930-11949 | MS.gene058520:CDS | 35.0% | |
| ATTCACTGTATTGAACGAGT+TGG | - | 50226:11783-11802 | MS.gene058520:CDS | 35.0% | |
| CCAAATGGGAAAAATGATCT+AGG | + | 50226:11043-11062 | MS.gene058520:intergenic | 35.0% | |
| CTACAACACCTTGGATAATA+GGG | + | 50226:11840-11859 | MS.gene058520:intergenic | 35.0% | |
| GAAATGGATAGATGTGTACT+TGG | - | 50226:11300-11319 | MS.gene058520:CDS | 35.0% | |
| TAAAAAGTCAGCAAAAGGGA+AGG | - | 50226:11567-11586 | MS.gene058520:CDS | 35.0% | |
| TAAGTTATCTCCACAACTGT+TGG | - | 50226:11096-11115 | MS.gene058520:CDS | 35.0% | |
| TCTACAACACCTTGGATAAT+AGG | + | 50226:11841-11860 | MS.gene058520:intergenic | 35.0% | |
| ! | AACAACTTCATCCTTGCTTT+TGG | + | 50226:11176-11195 | MS.gene058520:intergenic | 35.0% |
| ! | TGAAAATTGTGGCAGCATTT+TGG | - | 50226:11510-11529 | MS.gene058520:CDS | 35.0% |
| ! | TGCCACAATTTTCAATTCTC+AGG | + | 50226:11504-11523 | MS.gene058520:intergenic | 35.0% |
| !! | ACAACTTCATCCTTGCTTTT+GGG | + | 50226:11175-11194 | MS.gene058520:intergenic | 35.0% |
| !! | ATGAAGTTGTTAGCTTGTCT+TGG | - | 50226:11185-11204 | MS.gene058520:CDS | 35.0% |
| !! | CCTAGATCATTTTTCCCATT+TGG | - | 50226:11040-11059 | MS.gene058520:CDS | 35.0% |
| !! | GTTTTGATGCAAGCTATCTA+TGG | - | 50226:11589-11608 | MS.gene058520:CDS | 35.0% |
| ACGTGCACTGAAGTAAAAGA+TGG | + | 50226:11393-11412 | MS.gene058520:intergenic | 40.0% | |
| AGATCTTCTTACAAAGGAGG+TGG | - | 50226:11936-11955 | MS.gene058520:CDS | 40.0% | |
| AGGCTCTACAAAGAACTTGA+TGG | - | 50226:11663-11682 | MS.gene058520:CDS | 40.0% | |
| ATCCGGAAAGGATTTCCAAA+TGG | + | 50226:11058-11077 | MS.gene058520:intergenic | 40.0% | |
| CAATGGTATCTACAACACCT+TGG | + | 50226:11849-11868 | MS.gene058520:intergenic | 40.0% | |
| CGCATGTGATTCTGTTTCAA+TGG | + | 50226:11866-11885 | MS.gene058520:intergenic | 40.0% | |
| GAAAAGCTGATCTGATGAGA+AGG | + | 50226:11431-11450 | MS.gene058520:intergenic | 40.0% | |
| GATAAAACTGCCAACAGTTG+TGG | + | 50226:11109-11128 | MS.gene058520:intergenic | 40.0% | |
| GCAACAGAGAATTTCTCTCA+AGG | - | 50226:11910-11929 | MS.gene058520:CDS | 40.0% | |
| TCCCATTTGGAAATCCTTTC+CGG | - | 50226:11053-11072 | MS.gene058520:CDS | 40.0% | |
| TCCGGAAAGGATTTCCAAAT+GGG | + | 50226:11057-11076 | MS.gene058520:intergenic | 40.0% | |
| TGACTGATTGGGATGAGAAA+TGG | - | 50226:11284-11303 | MS.gene058520:CDS | 40.0% | |
| TTTCTCATCCCAATCAGTCA+CGG | + | 50226:11284-11303 | MS.gene058520:intergenic | 40.0% | |
| ! | AGCATTTTGGATAATCTGGC+TGG | - | 50226:11523-11542 | MS.gene058520:CDS | 40.0% |
| ! | CATGTTCTTCACTTGATGGT+TGG | - | 50226:11455-11474 | MS.gene058520:CDS | 40.0% |
| ! | GAACTTGATGGATATGGATG+TGG | - | 50226:11675-11694 | MS.gene058520:CDS | 40.0% |
| ! | GCATTTTGGATAATCTGGCT+GGG | - | 50226:11524-11543 | MS.gene058520:CDS | 40.0% |
| ! | TAGCTTGTCTTGGATGACTT+CGG | - | 50226:11195-11214 | MS.gene058520:CDS | 40.0% |
| ! | TCTCATCAGATCAGCTTTTC+CGG | - | 50226:11431-11450 | MS.gene058520:CDS | 40.0% |
| ! | TCTTGGGCTCCAACTTTTAA+AGG | - | 50226:11709-11728 | MS.gene058520:CDS | 40.0% |
| ! | TGGCAGCATTTTGGATAATC+TGG | - | 50226:11519-11538 | MS.gene058520:CDS | 40.0% |
| !! | GGCAGTTTTATCTGCTTTTG+AGG | - | 50226:11117-11136 | MS.gene058520:CDS | 40.0% |
| !!! | ATCTGCTTTTGAGGCAACTT+TGG | - | 50226:11126-11145 | MS.gene058520:CDS | 40.0% |
| !!! | CTTTTGAGGCAACTTTGGAA+GGG | - | 50226:11131-11150 | MS.gene058520:CDS | 40.0% |
| !!! | GTGTTTGCAACTGCTTTTTC+AGG | - | 50226:11643-11662 | MS.gene058520:CDS | 40.0% |
| !!! | TTTTTCCAAGTGGTCTTGTC+TGG | - | 50226:11964-11983 | MS.gene058520:CDS | 40.0% |
| AAACCTCACTCCCAAAAGCA+AGG | - | 50226:11162-11181 | MS.gene058520:CDS | 45.0% | |
| AAGCTCAAGGTCTGTCATCA+GGG | + | 50226:11260-11279 | MS.gene058520:intergenic | 45.0% | |
| AATGATCTAGGTGGATCCTG+TGG | + | 50226:11031-11050 | MS.gene058520:intergenic | 45.0% | |
| ACTGTCAATGACCACGTCTT+AGG | - | 50226:12024-12043 | MS.gene058520:CDS | 45.0% | |
| ATGTGGCTGATGTGTACTCT+TGG | - | 50226:11692-11711 | MS.gene058520:CDS | 45.0% | |
| CAGTGCACGTTGCACAATTT+AGG | - | 50226:11403-11422 | MS.gene058520:CDS | 45.0% | |
| CATCAAGTGAAGAACATGCC+CGG | + | 50226:11453-11472 | MS.gene058520:intergenic | 45.0% | |
| CTGTCAATGACCACGTCTTA+GGG | - | 50226:12025-12044 | MS.gene058520:CDS | 45.0% | |
| CTTCTTACAAAGGAGGTGGA+TGG | - | 50226:11940-11959 | MS.gene058520:CDS | 45.0% | |
| GCAAGCTATCTATGGAGTGA+AGG | - | 50226:11597-11616 | MS.gene058520:CDS | 45.0% | |
| TGACCTCAGATTAGACAGCA+AGG | + | 50226:11998-12017 | MS.gene058520:intergenic | 45.0% | |
| TGCCTTTAGCTGCTATCATC+CGG | + | 50226:11075-11094 | MS.gene058520:intergenic | 45.0% | |
| TGGGTCATCTGATCTTCCAA+AGG | - | 50226:11543-11562 | MS.gene058520:CDS | 45.0% | |
| TGTCAATGACCACGTCTTAG+GGG | - | 50226:12026-12045 | MS.gene058520:CDS | 45.0% | |
| TGTGGCTGATGTGTACTCTT+GGG | - | 50226:11693-11712 | MS.gene058520:CDS | 45.0% | |
| TTAGCTGCTATCATCCGGAA+AGG | + | 50226:11070-11089 | MS.gene058520:intergenic | 45.0% | |
| TTCCGGATGATAGCAGCTAA+AGG | - | 50226:11070-11089 | MS.gene058520:CDS | 45.0% | |
| TTCTCATCCCAATCAGTCAC+GGG | + | 50226:11283-11302 | MS.gene058520:intergenic | 45.0% | |
| ! | ATGCCTTGCTGTCTAATCTG+AGG | - | 50226:11992-12011 | MS.gene058520:CDS | 45.0% |
| ! | CTCATCAGATCAGCTTTTCC+GGG | - | 50226:11432-11451 | MS.gene058520:CDS | 45.0% |
| ! | GAGTTATCACGTCTGAAGCA+GGG | - | 50226:11367-11386 | MS.gene058520:CDS | 45.0% |
| ! | GTTCTTCACTTGATGGTTGG+AGG | - | 50226:11458-11477 | MS.gene058520:CDS | 45.0% |
| ! | TGAGTTATCACGTCTGAAGC+AGG | - | 50226:11366-11385 | MS.gene058520:CDS | 45.0% |
| ! | TTGCTGTCTAATCTGAGGTC+AGG | - | 50226:11997-12016 | MS.gene058520:CDS | 45.0% |
| !! | AAGCTGTTGATTTGTCTGCG+AGG | - | 50226:11806-11825 | MS.gene058520:CDS | 45.0% |
| !! | AGCTGTTGATTTGTCTGCGA+GGG | - | 50226:11807-11826 | MS.gene058520:CDS | 45.0% |
| !!! | GCTTTTGAGGCAACTTTGGA+AGG | - | 50226:11130-11149 | MS.gene058520:CDS | 45.0% |
| AATCAGTCACGGGAAGCTCA+AGG | + | 50226:11273-11292 | MS.gene058520:intergenic | 50.0% | |
| CGGGCATGTTCTTCACTTGA+TGG | - | 50226:11451-11470 | MS.gene058520:CDS | 50.0% | |
| CTTTGTTACCCCCTAAGACG+TGG | + | 50226:12038-12057 | MS.gene058520:intergenic | 50.0% | |
| GAAGCTCAAGGTCTGTCATC+AGG | + | 50226:11261-11280 | MS.gene058520:intergenic | 50.0% | |
| GATGGTTGGAGGCAGCATAT+TGG | - | 50226:11469-11488 | MS.gene058520:CDS | 50.0% | |
| GCGAAGACTGTTGAGAAGCT+AGG | - | 50226:11883-11902 | MS.gene058520:CDS | 50.0% | |
| GTCAATGACCACGTCTTAGG+GGG | - | 50226:12027-12046 | MS.gene058520:CDS | 50.0% | |
| TGAGCTTCCCGTGACTGATT+GGG | - | 50226:11273-11292 | MS.gene058520:CDS | 50.0% | |
| TTGAGCTTCCCGTGACTGAT+TGG | - | 50226:11272-11291 | MS.gene058520:CDS | 50.0% | |
| TTGGATGACTTCGGCTATGC+AGG | - | 50226:11204-11223 | MS.gene058520:CDS | 50.0% | |
| ! | TTGAGGCAACTTTGGAAGGG+AGG | - | 50226:11134-11153 | MS.gene058520:CDS | 50.0% |
| !! | CATCCTTGCTTTTGGGAGTG+AGG | + | 50226:11168-11187 | MS.gene058520:intergenic | 50.0% |
| !! | GGTGGATGGCTTTTTCCAAG+TGG | - | 50226:11954-11973 | MS.gene058520:CDS | 50.0% |
| CACGACCAGACAAGACCACT+TGG | + | 50226:11972-11991 | MS.gene058520:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50226 | gene | 11019 | 12077 | 11019 | ID=MS.gene058520 |
| 50226 | mRNA | 11019 | 12077 | 11019 | ID=MS.gene058520.t1;Parent=MS.gene058520 |
| 50226 | exon | 11019 | 12077 | 11019 | ID=MS.gene058520.t1.exon1;Parent=MS.gene058520.t1 |
| 50226 | CDS | 11019 | 12077 | 11019 | ID=cds.MS.gene058520.t1;Parent=MS.gene058520.t1 |
| Gene Sequence |
| Protein sequence |
