Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52124.t1 | PNY05923.1 | 90.1 | 181 | 18 | 0 | 1 | 181 | 1 | 181 | 3.30E-83 | 317.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52124.t1 | Q54CI5 | 29.9 | 184 | 86 | 5 | 10 | 178 | 14 | 169 | 1.9e-10 | 67.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52124.t1 | A0A2K3NSB6 | 90.1 | 181 | 18 | 0 | 1 | 181 | 1 | 181 | 2.4e-83 | 317.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057659 | MS.gene52124 | 0.837465 | 5.12E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene52124 | MS.gene83474 | PPI |
| MS.gene52124 | MS.gene72809 | PPI |
| MS.gene52124 | MS.gene82121 | PPI |
| MS.gene52124 | MS.gene028111 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52124.t1 | MTR_3g006810 | 82.320 | 181 | 29 | 2 | 1 | 181 | 1 | 178 | 3.33e-102 | 291 |
| MS.gene52124.t1 | MTR_6g003930 | 81.215 | 181 | 31 | 2 | 1 | 181 | 1 | 178 | 1.67e-100 | 287 |
| MS.gene52124.t1 | MTR_6g003930 | 81.215 | 181 | 31 | 2 | 1 | 181 | 73 | 250 | 3.56e-99 | 286 |
| MS.gene52124.t1 | MTR_4g074580 | 95.489 | 133 | 6 | 0 | 1 | 133 | 1 | 133 | 5.52e-90 | 259 |
| MS.gene52124.t1 | MTR_7g112360 | 28.030 | 132 | 85 | 4 | 12 | 137 | 17 | 144 | 7.57e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52124.t1 | AT4G10130 | 52.874 | 174 | 72 | 3 | 1 | 174 | 1 | 164 | 2.88e-59 | 183 |
| MS.gene52124.t1 | AT4G10130 | 52.874 | 174 | 72 | 3 | 1 | 174 | 1 | 164 | 2.88e-59 | 183 |
| MS.gene52124.t1 | AT4G10130 | 52.874 | 174 | 72 | 3 | 1 | 174 | 1 | 164 | 2.88e-59 | 183 |
| MS.gene52124.t1 | AT4G10130 | 52.874 | 174 | 72 | 3 | 1 | 174 | 1 | 164 | 2.88e-59 | 183 |
| MS.gene52124.t1 | AT4G10130 | 52.874 | 174 | 72 | 3 | 1 | 174 | 1 | 164 | 2.88e-59 | 183 |
| MS.gene52124.t1 | AT1G56300 | 27.273 | 132 | 80 | 4 | 7 | 129 | 9 | 133 | 2.33e-11 | 59.3 |
| MS.gene52124.t1 | AT3G13310 | 37.662 | 77 | 38 | 1 | 13 | 89 | 66 | 132 | 4.69e-11 | 58.5 |
Find 32 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAAGTTGCAGAAGATTTA+AGG | 0.106422 | 4.1:-29483650 | MS.gene52124:CDS |
| CTCATTGGAATTGCAGAAAA+TGG | 0.178941 | 4.1:-29483544 | MS.gene52124:CDS |
| TGCAGAAGATTTAAGGTTAC+AGG | 0.248624 | 4.1:-29483643 | MS.gene52124:CDS |
| TTCAAGGCGGGAGGATTTCT+TGG | 0.279453 | 4.1:-29483676 | MS.gene52124:CDS |
| GATGTCTTTAATAGTTTATC+AGG | 0.288420 | 4.1:+29483813 | None:intergenic |
| GAAATCTCTCTGAAGTTGTT+TGG | 0.379708 | 4.1:+29483772 | None:intergenic |
| CTTAATGTTGATACTTTACC+AGG | 0.381509 | 4.1:-29483477 | MS.gene52124:CDS |
| TCATTGGAATTGCAGAAAAT+GGG | 0.406489 | 4.1:-29483543 | MS.gene52124:CDS |
| AGCTCGTCTTGTACTCGGTA+TGG | 0.418652 | 4.1:-29483418 | MS.gene52124:CDS |
| TTCTCAAAGTTCACAAGGCA+TGG | 0.443232 | 4.1:-29483752 | MS.gene52124:CDS |
| TTGAAAGCTCGTCTTGTACT+CGG | 0.465157 | 4.1:-29483423 | MS.gene52124:CDS |
| CTCTCGTTTAATTTATGACA+AGG | 0.490736 | 4.1:-29483709 | MS.gene52124:CDS |
| ACAAGGAACTGAAAATTTCA+AGG | 0.503255 | 4.1:-29483692 | MS.gene52124:CDS |
| GCTCGTCTTGTACTCGGTAT+GGG | 0.524137 | 4.1:-29483417 | MS.gene52124:CDS |
| CAAGGAAGAATCACTGATCC+TGG | 0.528820 | 4.1:+29483459 | None:intergenic |
| TCTCAAAGTTCACAAGGCAT+GGG | 0.543905 | 4.1:-29483751 | MS.gene52124:CDS |
| GGAACTGAAAATTTCAAGGC+GGG | 0.548219 | 4.1:-29483688 | MS.gene52124:CDS |
| TGAAGATGCTGGTGAAACAT+TGG | 0.562066 | 4.1:-29483610 | MS.gene52124:CDS |
| CTTGTCATAAATTAAACGAG+AGG | 0.568113 | 4.1:+29483710 | None:intergenic |
| GGATCAGTGATTCTTCCTTG+TGG | 0.587887 | 4.1:-29483456 | MS.gene52124:CDS |
| CAGCACTTCGGTAACTAGCA+CGG | 0.609445 | 4.1:+29483850 | None:intergenic |
| ACTGAAAATTTCAAGGCGGG+AGG | 0.610139 | 4.1:-29483685 | MS.gene52124:CDS |
| CATTGGAATTGCAGAAAATG+GGG | 0.618046 | 4.1:-29483542 | MS.gene52124:CDS |
| TTACGAGGTTCTCAATGTCA+AGG | 0.619850 | 4.1:-29483895 | MS.gene52124:CDS |
| CATTGAGGAGACTCATTACG+AGG | 0.636562 | 4.1:-29483910 | MS.gene52124:CDS |
| GAGATTTCTCAAAGTTCACA+AGG | 0.644008 | 4.1:-29483757 | MS.gene52124:CDS |
| GTAAACTGAGTGCAGCACTT+CGG | 0.645782 | 4.1:+29483838 | None:intergenic |
| GCTGTCTTCATCTCTCAACA+AGG | 0.680466 | 4.1:+29483515 | None:intergenic |
| GATATGACAGTTGAAGATGC+TGG | 0.682484 | 4.1:-29483621 | MS.gene52124:CDS |
| CGAGGTTCTCAATGTCAAGG+AGG | 0.689102 | 4.1:-29483892 | MS.gene52124:CDS |
| AGGAACTGAAAATTTCAAGG+CGG | 0.730120 | 4.1:-29483689 | MS.gene52124:CDS |
| TTCAACGAACAAGATCCACA+AGG | 0.762477 | 4.1:+29483441 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATGTCTTTAATAGTTTATC+AGG | + | chr4.1:29483527-29483546 | None:intergenic | 25.0% |
| ACAAGGAACTGAAAATTTCA+AGG | - | chr4.1:29483645-29483664 | MS.gene52124:CDS | 30.0% | |
| CTTAATGTTGATACTTTACC+AGG | - | chr4.1:29483860-29483879 | MS.gene52124:CDS | 30.0% | |
| CTTGTCATAAATTAAACGAG+AGG | + | chr4.1:29483630-29483649 | None:intergenic | 30.0% | |
| TCATTGGAATTGCAGAAAAT+GGG | - | chr4.1:29483794-29483813 | MS.gene52124:CDS | 30.0% | |
| TTTGGACAAGAAAAACATTG+AGG | - | chr4.1:29483412-29483431 | MS.gene52124:CDS | 30.0% | |
| ! | CTCTCGTTTAATTTATGACA+AGG | - | chr4.1:29483628-29483647 | MS.gene52124:CDS | 30.0% |
| !! | TTGTTTTATCAATGTCGATG+CGG | - | chr4.1:29483752-29483771 | MS.gene52124:CDS | 30.0% |
| AGGAACTGAAAATTTCAAGG+CGG | - | chr4.1:29483648-29483667 | MS.gene52124:CDS | 35.0% | |
| CATTGGAATTGCAGAAAATG+GGG | - | chr4.1:29483795-29483814 | MS.gene52124:CDS | 35.0% | |
| CTCATTGGAATTGCAGAAAA+TGG | - | chr4.1:29483793-29483812 | MS.gene52124:CDS | 35.0% | |
| CTGAAGTTGCAGAAGATTTA+AGG | - | chr4.1:29483687-29483706 | MS.gene52124:CDS | 35.0% | |
| GAGATTTCTCAAAGTTCACA+AGG | - | chr4.1:29483580-29483599 | MS.gene52124:CDS | 35.0% | |
| ! | TGCAGAAGATTTAAGGTTAC+AGG | - | chr4.1:29483694-29483713 | MS.gene52124:CDS | 35.0% |
| !! | GAAATCTCTCTGAAGTTGTT+TGG | + | chr4.1:29483568-29483587 | None:intergenic | 35.0% |
| !! | TTACTTTTCTGTGGACTCAT+TGG | - | chr4.1:29483778-29483797 | MS.gene52124:CDS | 35.0% |
| GATATGACAGTTGAAGATGC+TGG | - | chr4.1:29483716-29483735 | MS.gene52124:CDS | 40.0% | |
| GGAACTGAAAATTTCAAGGC+GGG | - | chr4.1:29483649-29483668 | MS.gene52124:CDS | 40.0% | |
| TCTCAAAGTTCACAAGGCAT+GGG | - | chr4.1:29483586-29483605 | MS.gene52124:CDS | 40.0% | |
| TGAAGATGCTGGTGAAACAT+TGG | - | chr4.1:29483727-29483746 | MS.gene52124:CDS | 40.0% | |
| TTACGAGGTTCTCAATGTCA+AGG | - | chr4.1:29483442-29483461 | MS.gene52124:CDS | 40.0% | |
| TTCAACGAACAAGATCCACA+AGG | + | chr4.1:29483899-29483918 | None:intergenic | 40.0% | |
| TTCTCAAAGTTCACAAGGCA+TGG | - | chr4.1:29483585-29483604 | MS.gene52124:CDS | 40.0% | |
| TTGAAAGCTCGTCTTGTACT+CGG | - | chr4.1:29483914-29483933 | MS.gene52124:CDS | 40.0% | |
| !! | ATGCGGTGATTACTTTTCTG+TGG | - | chr4.1:29483769-29483788 | MS.gene52124:CDS | 40.0% |
| ACTGAAAATTTCAAGGCGGG+AGG | - | chr4.1:29483652-29483671 | MS.gene52124:CDS | 45.0% | |
| CAAGGAAGAATCACTGATCC+TGG | + | chr4.1:29483881-29483900 | None:intergenic | 45.0% | |
| CATTGAGGAGACTCATTACG+AGG | - | chr4.1:29483427-29483446 | MS.gene52124:CDS | 45.0% | |
| GCTGTCTTCATCTCTCAACA+AGG | + | chr4.1:29483825-29483844 | None:intergenic | 45.0% | |
| GGATCAGTGATTCTTCCTTG+TGG | - | chr4.1:29483881-29483900 | MS.gene52124:CDS | 45.0% | |
| GTAAACTGAGTGCAGCACTT+CGG | + | chr4.1:29483502-29483521 | None:intergenic | 45.0% | |
| AGCTCGTCTTGTACTCGGTA+TGG | - | chr4.1:29483919-29483938 | MS.gene52124:CDS | 50.0% | |
| CAGCACTTCGGTAACTAGCA+CGG | + | chr4.1:29483490-29483509 | None:intergenic | 50.0% | |
| CGAGGTTCTCAATGTCAAGG+AGG | - | chr4.1:29483445-29483464 | MS.gene52124:CDS | 50.0% | |
| GCTCGTCTTGTACTCGGTAT+GGG | - | chr4.1:29483920-29483939 | MS.gene52124:CDS | 50.0% | |
| TTCAAGGCGGGAGGATTTCT+TGG | - | chr4.1:29483661-29483680 | MS.gene52124:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 29483407 | 29483952 | 29483407 | ID=MS.gene52124 |
| chr4.1 | mRNA | 29483407 | 29483952 | 29483407 | ID=MS.gene52124.t1;Parent=MS.gene52124 |
| chr4.1 | exon | 29483407 | 29483952 | 29483407 | ID=MS.gene52124.t1.exon1;Parent=MS.gene52124.t1 |
| chr4.1 | CDS | 29483407 | 29483952 | 29483407 | ID=cds.MS.gene52124.t1;Parent=MS.gene52124.t1 |
| Gene Sequence |
| Protein sequence |
