Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53954.t1 | XP_013458798.1 | 91.6 | 178 | 15 | 0 | 1 | 178 | 1 | 178 | 4.30E-83 | 317.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53954.t1 | Q54CI5 | 28.6 | 182 | 90 | 4 | 10 | 176 | 14 | 170 | 3.2e-10 | 66.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53954.t1 | A0A072V3W3 | 91.6 | 178 | 15 | 0 | 1 | 178 | 1 | 178 | 3.1e-83 | 317.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene53954 | MS.gene82121 | PPI |
| MS.gene53954 | MS.gene028111 | PPI |
| MS.gene53954 | MS.gene83474 | PPI |
| MS.gene53954 | MS.gene72809 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53954.t1 | MTR_3g006810 | 91.573 | 178 | 15 | 0 | 1 | 178 | 1 | 178 | 4.01e-118 | 332 |
| MS.gene53954.t1 | MTR_6g003930 | 90.449 | 178 | 17 | 0 | 1 | 178 | 1 | 178 | 1.36e-116 | 328 |
| MS.gene53954.t1 | MTR_6g003930 | 90.449 | 178 | 17 | 0 | 1 | 178 | 73 | 250 | 3.80e-115 | 327 |
| MS.gene53954.t1 | MTR_4g074580 | 78.947 | 133 | 27 | 1 | 1 | 132 | 1 | 133 | 1.08e-69 | 207 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53954.t1 | AT4G10130 | 50.000 | 176 | 81 | 2 | 1 | 176 | 1 | 169 | 1.58e-57 | 178 |
| MS.gene53954.t1 | AT4G10130 | 50.000 | 176 | 81 | 2 | 1 | 176 | 1 | 169 | 1.58e-57 | 178 |
| MS.gene53954.t1 | AT4G10130 | 50.000 | 176 | 81 | 2 | 1 | 176 | 1 | 169 | 1.58e-57 | 178 |
| MS.gene53954.t1 | AT4G10130 | 50.000 | 176 | 81 | 2 | 1 | 176 | 1 | 169 | 1.58e-57 | 178 |
| MS.gene53954.t1 | AT4G10130 | 50.000 | 176 | 81 | 2 | 1 | 176 | 1 | 169 | 1.58e-57 | 178 |
Find 34 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGAAGATTTAAGCTTAC+AGG | 0.214140 | 3.2:+1528267 | MS.gene53954:CDS |
| ACTATTTCCATTTCTCAATA+AGG | 0.240811 | 3.2:-1528395 | None:intergenic |
| CTCGTTGGAATTGCTGAAAA+TGG | 0.258071 | 3.2:+1528366 | MS.gene53954:CDS |
| AGATTTAAGCTTACAGGATA+TGG | 0.297921 | 3.2:+1528273 | MS.gene53954:CDS |
| GCTCCTTATCATATAATAAA+CGG | 0.369390 | 3.2:-1528207 | None:intergenic |
| AAGAAATTCGTGCTAGTTAC+CGG | 0.375273 | 3.2:+1528056 | MS.gene53954:CDS |
| ATCCCGTTTATTATATGATA+AGG | 0.385875 | 3.2:+1528204 | MS.gene53954:CDS |
| GGGTATTCCTTATTGAGAAA+TGG | 0.387591 | 3.2:+1528388 | MS.gene53954:CDS |
| TCGTTGGAATTGCTGAAAAT+GGG | 0.404006 | 3.2:+1528367 | MS.gene53954:CDS |
| TTATGAGGTTCTCAATGTTA+AGG | 0.412571 | 3.2:+1528018 | MS.gene53954:CDS |
| CTCAATGGTGATACTTTACC+AGG | 0.416326 | 3.2:+1528427 | MS.gene53954:CDS |
| AGCTCGTCTGATACTCAGTA+TGG | 0.427425 | 3.2:+1528486 | MS.gene53954:CDS |
| CTCCTTATCATATAATAAAC+GGG | 0.433842 | 3.2:-1528206 | None:intergenic |
| GAGATTTCTCAAAGTTCAAA+AGG | 0.446313 | 3.2:+1528156 | MS.gene53954:CDS |
| TTCTCAAAGTTCAAAAGGCA+TGG | 0.453193 | 3.2:+1528161 | MS.gene53954:CDS |
| CGTACTTGTTTGATCACTGT+TGG | 0.461429 | 3.2:-1528131 | None:intergenic |
| CATTGAGGAAACTCATTATG+AGG | 0.462336 | 3.2:+1528003 | MS.gene53954:CDS |
| AAGATCAAGGCGGGATGCCT+TGG | 0.472867 | 3.2:+1528234 | MS.gene53954:CDS |
| CAAGGAAGAATCACGGATCC+TGG | 0.489467 | 3.2:-1528445 | None:intergenic |
| TTCTGCAATTTCAGCAGCCA+AGG | 0.518010 | 3.2:-1528251 | None:intergenic |
| AATAGTATATCTATACTCAA+TGG | 0.536490 | 3.2:+1528412 | MS.gene53954:CDS |
| TCTTGACAAGCATGACATTG+AGG | 0.539456 | 3.2:+1527988 | MS.gene53954:CDS |
| TCTCAAAGTTCAAAAGGCAT+GGG | 0.541609 | 3.2:+1528162 | MS.gene53954:CDS |
| TTACTTCTCTCTCGACTCGT+TGG | 0.542824 | 3.2:+1528351 | MS.gene53954:CDS |
| ATAAGGAGCTGCAAAGATCA+AGG | 0.544696 | 3.2:+1528221 | MS.gene53954:CDS |
| GGAGCTGCAAAGATCAAGGC+GGG | 0.575943 | 3.2:+1528225 | MS.gene53954:CDS |
| GGATCCGTGATTCTTCCTTG+TGG | 0.587718 | 3.2:+1528448 | MS.gene53954:CDS |
| CAACAGTGATCAAACAAGTA+CGG | 0.595965 | 3.2:+1528132 | MS.gene53954:CDS |
| CGTTGGAATTGCTGAAAATG+GGG | 0.627347 | 3.2:+1528368 | MS.gene53954:CDS |
| AGATCCACAAGGAAGAATCA+CGG | 0.662581 | 3.2:-1528452 | None:intergenic |
| GAGAGAAGTAATCACCACAT+CGG | 0.695257 | 3.2:-1528339 | None:intergenic |
| GTAAACAGAGTACAGCACTC+CGG | 0.713129 | 3.2:-1528075 | None:intergenic |
| TTTAACGAACAAGATCCACA+AGG | 0.727941 | 3.2:-1528463 | None:intergenic |
| AGGAGCTGCAAAGATCAAGG+CGG | 0.734461 | 3.2:+1528224 | MS.gene53954:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAGTATATCTATACTCAA+TGG | + | chr3.2:1528412-1528431 | MS.gene53954:CDS | 20.0% |
| ! | ACTATTTCCATTTCTCAATA+AGG | - | chr3.2:1528398-1528417 | None:intergenic | 25.0% |
| ! | ATCCCGTTTATTATATGATA+AGG | + | chr3.2:1528204-1528223 | MS.gene53954:CDS | 25.0% |
| ! | CTCCTTATCATATAATAAAC+GGG | - | chr3.2:1528209-1528228 | None:intergenic | 25.0% |
| ! | GCTCCTTATCATATAATAAA+CGG | - | chr3.2:1528210-1528229 | None:intergenic | 25.0% |
| AGATTTAAGCTTACAGGATA+TGG | + | chr3.2:1528273-1528292 | MS.gene53954:CDS | 30.0% | |
| GAGATTTCTCAAAGTTCAAA+AGG | + | chr3.2:1528156-1528175 | MS.gene53954:CDS | 30.0% | |
| TTATGAGGTTCTCAATGTTA+AGG | + | chr3.2:1528018-1528037 | MS.gene53954:CDS | 30.0% | |
| AAGAAATTCGTGCTAGTTAC+CGG | + | chr3.2:1528056-1528075 | MS.gene53954:CDS | 35.0% | |
| CAACAGTGATCAAACAAGTA+CGG | + | chr3.2:1528132-1528151 | MS.gene53954:CDS | 35.0% | |
| CATTGAGGAAACTCATTATG+AGG | + | chr3.2:1528003-1528022 | MS.gene53954:CDS | 35.0% | |
| GGGTATTCCTTATTGAGAAA+TGG | + | chr3.2:1528388-1528407 | MS.gene53954:CDS | 35.0% | |
| TCGTTGGAATTGCTGAAAAT+GGG | + | chr3.2:1528367-1528386 | MS.gene53954:CDS | 35.0% | |
| TGCAGAAGATTTAAGCTTAC+AGG | + | chr3.2:1528267-1528286 | MS.gene53954:CDS | 35.0% | |
| TTCTCAAAGTTCAAAAGGCA+TGG | + | chr3.2:1528161-1528180 | MS.gene53954:CDS | 35.0% | |
| TTTAACGAACAAGATCCACA+AGG | - | chr3.2:1528466-1528485 | None:intergenic | 35.0% | |
| ! | TCTCAAAGTTCAAAAGGCAT+GGG | + | chr3.2:1528162-1528181 | MS.gene53954:CDS | 35.0% |
| AGATCCACAAGGAAGAATCA+CGG | - | chr3.2:1528455-1528474 | None:intergenic | 40.0% | |
| ATAAGGAGCTGCAAAGATCA+AGG | + | chr3.2:1528221-1528240 | MS.gene53954:CDS | 40.0% | |
| CGTACTTGTTTGATCACTGT+TGG | - | chr3.2:1528134-1528153 | None:intergenic | 40.0% | |
| CGTTGGAATTGCTGAAAATG+GGG | + | chr3.2:1528368-1528387 | MS.gene53954:CDS | 40.0% | |
| CTCAATGGTGATACTTTACC+AGG | + | chr3.2:1528427-1528446 | MS.gene53954:CDS | 40.0% | |
| CTCGTTGGAATTGCTGAAAA+TGG | + | chr3.2:1528366-1528385 | MS.gene53954:CDS | 40.0% | |
| GAGAGAAGTAATCACCACAT+CGG | - | chr3.2:1528342-1528361 | None:intergenic | 40.0% | |
| TCTTGACAAGCATGACATTG+AGG | + | chr3.2:1527988-1528007 | MS.gene53954:CDS | 40.0% | |
| ! | CTGTTTTATCAATGCCGATG+TGG | + | chr3.2:1528325-1528344 | MS.gene53954:CDS | 40.0% |
| AGCTCGTCTGATACTCAGTA+TGG | + | chr3.2:1528486-1528505 | MS.gene53954:CDS | 45.0% | |
| GTAAACAGAGTACAGCACTC+CGG | - | chr3.2:1528078-1528097 | None:intergenic | 45.0% | |
| TTCTGCAATTTCAGCAGCCA+AGG | - | chr3.2:1528254-1528273 | None:intergenic | 45.0% | |
| ! | TTACTTCTCTCTCGACTCGT+TGG | + | chr3.2:1528351-1528370 | MS.gene53954:CDS | 45.0% |
| AGGAGCTGCAAAGATCAAGG+CGG | + | chr3.2:1528224-1528243 | MS.gene53954:CDS | 50.0% | |
| CAAGGAAGAATCACGGATCC+TGG | - | chr3.2:1528448-1528467 | None:intergenic | 50.0% | |
| GGATCCGTGATTCTTCCTTG+TGG | + | chr3.2:1528448-1528467 | MS.gene53954:CDS | 50.0% | |
| AAGATCAAGGCGGGATGCCT+TGG | + | chr3.2:1528234-1528253 | MS.gene53954:CDS | 55.0% | |
| GGAGCTGCAAAGATCAAGGC+GGG | + | chr3.2:1528225-1528244 | MS.gene53954:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 1527983 | 1528519 | 1527983 | ID=MS.gene53954 |
| chr3.2 | mRNA | 1527983 | 1528519 | 1527983 | ID=MS.gene53954.t1;Parent=MS.gene53954 |
| chr3.2 | exon | 1527983 | 1528519 | 1527983 | ID=MS.gene53954.t1.exon1;Parent=MS.gene53954.t1 |
| chr3.2 | CDS | 1527983 | 1528519 | 1527983 | ID=cds.MS.gene53954.t1;Parent=MS.gene53954.t1 |
| Gene Sequence |
| Protein sequence |
