AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54077

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54077.t1 AT2G24440 63.529 85 28 2 124 207 100 182 9.69e-34 119
MS.gene54077.t1 AT4G31360 43.871 155 62 3 52 204 51 182 6.76e-29 107

Find 66 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATACATTCATTAGCCTTT+TGG 0.252653 1.2:+10221585 MS.gene54077:CDS
GGGTCATCATCTTTGTCTTC+AGG 0.263558 1.2:-10218215 None:intergenic
AAGGCTGCGGCGCCGTCGCC+GGG 0.286937 1.2:+10217921 MS.gene54077:CDS
GAAGGCTGCGGCGCCGTCGC+CGG 0.317767 1.2:+10217920 MS.gene54077:CDS
GCTGACAGTGAAGGTGCTTC+TGG 0.354259 1.2:+10218101 MS.gene54077:CDS
ACGACGATAACAGTCCTGTT+AGG 0.360688 1.2:-10218236 None:intergenic
TGAATGTATGGCCACCTTGC+TGG 0.361738 1.2:-10221573 None:intergenic
CGAGACAAGCCTTGCACCTT+CGG 0.369026 1.2:-10217988 None:intergenic
AGATCCTTCATTGGCTTGAA+TGG 0.378234 1.2:-10221999 None:intergenic
GCTTCTGGTACTACTGGTTC+TGG 0.397333 1.2:+10218116 MS.gene54077:CDS
GCATCAGAACCTCCACCTTC+AGG 0.404405 1.2:-10218182 None:intergenic
ACAAAGATGATGACCCTAAC+AGG 0.405557 1.2:+10218222 MS.gene54077:CDS
GAAAGCTGAACCGAAGGATA+AGG 0.411247 1.2:+10218067 MS.gene54077:CDS
CCAAAAGGCTAATGAATGTA+TGG 0.417197 1.2:-10221585 None:intergenic
GCCATGTCAAGATCCTTCAT+TGG 0.424164 1.2:-10222008 None:intergenic
CGCAGCCTTCGCCGGAGAAG+CGG 0.424554 1.2:-10217907 None:intergenic
GCTTTCTTTCCCTTATCCTT+CGG 0.424678 1.2:-10218077 None:intergenic
GACGGCGCCGCAGCCTTCGC+CGG 0.427048 1.2:-10217915 None:intergenic
TTCTTCTGCTTCTTCTTCGC+CGG 0.429999 1.2:-10218035 None:intergenic
CGCTTCTCCGGCGAAGGCTG+CGG 0.439074 1.2:+10217908 MS.gene54077:CDS
GTTGGAACGAAGTGTTCTTC+CGG 0.445327 1.2:-10217964 None:intergenic
TACTGAGGTGAAGGATGGTC+TGG 0.453806 1.2:+10219393 MS.gene54077:CDS
GAAGGTGCTTCTGGTACTAC+TGG 0.470059 1.2:+10218110 MS.gene54077:CDS
GGTACTACTGGTTCTGGTGG+TGG 0.473554 1.2:+10218122 MS.gene54077:CDS
GTTGCTGATTTATCAAATGC+TGG 0.479820 1.2:+10222050 MS.gene54077:CDS
CGACGATAACAGTCCTGTTA+GGG 0.488543 1.2:-10218235 None:intergenic
TGGTGCTGACCCGAAGGGTA+AGG 0.492398 1.2:+10218142 MS.gene54077:CDS
AAGCCTTGCACCTTCGGAGT+TGG 0.492453 1.2:-10217982 None:intergenic
CAAGGCTAAAACCGCTTCTC+CGG 0.494433 1.2:+10217896 MS.gene54077:CDS
AAAGCTGAACCGAAGGATAA+GGG 0.495239 1.2:+10218068 MS.gene54077:CDS
ATTTCAAAGCAACCTCTCCT+TGG 0.512327 1.2:-10221547 None:intergenic
AACAATATCTGCGATGACCT+TGG 0.514515 1.2:-10222030 None:intergenic
TAAAACCGCTTCTCCGGCGA+AGG 0.535908 1.2:+10217902 MS.gene54077:CDS
CTGTTATCGTCGTTGAGCAT+TGG 0.551171 1.2:+10218246 MS.gene54077:CDS
GAGGAGAGCTACTGAGGTGA+AGG 0.553058 1.2:+10219384 MS.gene54077:CDS
TCGTCCATTCAAGCCAATGA+AGG 0.555939 1.2:+10221995 MS.gene54077:CDS
TGGTGGTGGTGCTGACCCGA+AGG 0.556830 1.2:+10218136 MS.gene54077:CDS
GGTGCTGACCCGAAGGGTAA+GGG 0.558148 1.2:+10218143 MS.gene54077:CDS
GGCGAAGAAGAAGCAGAAGA+AGG 0.558274 1.2:+10218037 MS.gene54077:CDS
GTTCCAACTCCGAAGGTGCA+AGG 0.564313 1.2:+10217979 MS.gene54077:CDS
GAAGAAGAAAGCTGAACCGA+AGG 0.566958 1.2:+10218061 MS.gene54077:CDS
GCTGCGGCGCCGTCGCCGGG+AGG 0.581702 1.2:+10217924 MS.gene54077:CDS
CTCAATGCAAATCATTCTTG+AGG 0.583853 1.2:+10219365 MS.gene54077:CDS
GGTGAAACTGAACCCTGAAA+AGG 0.589350 1.2:+10219441 MS.gene54077:CDS
TGACATTCAGAAGAAATCTC+CGG 0.589803 1.2:+10218016 MS.gene54077:CDS
TGAGGTGAAGGATGGTCTGG+AGG 0.590927 1.2:+10219396 MS.gene54077:CDS
GAGGTGAAGGATGGTCTGGA+GGG 0.591530 1.2:+10219397 MS.gene54077:CDS
AGAGCTACTGAGGTGAAGGA+TGG 0.600555 1.2:+10219388 MS.gene54077:CDS
AAGAAAGCCGCTGACAGTGA+AGG 0.603856 1.2:+10218092 MS.gene54077:CDS
TGCTTTGAAATACGCCAGCA+AGG 0.604282 1.2:+10221559 MS.gene54077:CDS
GAAGGATCTTGACATGGCCA+AGG 0.612943 1.2:+10222013 MS.gene54077:CDS
AGAAATGGCACCAAAACGCA+AGG 0.626423 1.2:+10217878 None:intergenic
AAACAAGCCGCTGTACCTGA+AGG 0.636179 1.2:+10218167 MS.gene54077:CDS
GCCAATGAAGGATCTTGACA+TGG 0.636927 1.2:+10222007 MS.gene54077:CDS
GCCGCTGTACCTGAAGGTGG+AGG 0.642345 1.2:+10218173 MS.gene54077:CDS
GGAACGAAGTGTTCTTCCGG+AGG 0.643186 1.2:-10217961 None:intergenic
CAAGCCGCTGTACCTGAAGG+TGG 0.643554 1.2:+10218170 MS.gene54077:CDS
ATTCTTGAGGAGAGCTACTG+AGG 0.643723 1.2:+10219378 MS.gene54077:CDS
ACACTTCGTTCCAACTCCGA+AGG 0.650374 1.2:+10217972 MS.gene54077:CDS
TCTGGTACTACTGGTTCTGG+TGG 0.655303 1.2:+10218119 MS.gene54077:CDS
TAGTCAAGCTATTGATGTGG+TGG 0.665049 1.2:+10219420 MS.gene54077:CDS
GGTGGTGGTGCTGACCCGAA+GGG 0.687297 1.2:+10218137 MS.gene54077:CDS
ACCTCCACCTTCAGGTACAG+CGG 0.699979 1.2:-10218174 None:intergenic
GGGTAGTCAAGCTATTGATG+TGG 0.707014 1.2:+10219417 MS.gene54077:CDS
AGAAGCACCTTCACTGTCAG+CGG 0.707449 1.2:-10218099 None:intergenic
TTTGAAATACGCCAGCAAGG+TGG 0.712095 1.2:+10221562 MS.gene54077:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTTTTAAGGAAAAAAT+TGG - chr1.2:10221524-10221543 None:intergenic 10.0%
!! ATAAATGGAATATATTGAAA+TGG + chr1.2:10218717-10218736 MS.gene54077:intron 15.0%
!! GAAAAATGACTAAAAAATAA+TGG - chr1.2:10221728-10221747 None:intergenic 15.0%
!! TCAAAAAGAAATTGTAAAAA+AGG - chr1.2:10221405-10221424 None:intergenic 15.0%
!! TTGAAAACAAAATGTTTAAA+AGG - chr1.2:10221346-10221365 None:intergenic 15.0%
!!! AATTTTTCAATCCATTTTTT+CGG - chr1.2:10218660-10218679 None:intergenic 15.0%
!! ATGGAATATAGATAATGAAA+TGG + chr1.2:10218816-10218835 MS.gene54077:intron 20.0%
!! ATTGAATAGAGTGTAATAAT+TGG + chr1.2:10218965-10218984 MS.gene54077:intron 20.0%
!! GTTGATTAAAGTGTAATAAA+TGG + chr1.2:10218702-10218721 MS.gene54077:intron 20.0%
!! TAAAGTGGTAAAATAATCAA+TGG - chr1.2:10219596-10219615 None:intergenic 20.0%
!! TTATAACACATAATGTAAAG+TGG - chr1.2:10219611-10219630 None:intergenic 20.0%
!!! AAAGAAGCAAATTATTTTTC+AGG - chr1.2:10219113-10219132 None:intergenic 20.0%
!!! AATATTTTTCGTCATCATTA+CGG - chr1.2:10218481-10218500 None:intergenic 20.0%
!!! AATTTTTTATGTGGATAGTT+TGG + chr1.2:10218913-10218932 MS.gene54077:intron 20.0%
!!! ACAAATAGAAAAAAGTACAA+AGG - chr1.2:10221668-10221687 None:intergenic 20.0%
!!! CAACATGAACATTTTTTAAT+TGG + chr1.2:10221629-10221648 MS.gene54077:intron 20.0%
!!! TGAAGTTTTTTTGGATATAT+TGG + chr1.2:10221702-10221721 MS.gene54077:intron 20.0%
!!! TGCTATATTTGAAGTTTTTT+TGG + chr1.2:10221693-10221712 MS.gene54077:intron 20.0%
! AACATTGTGAAGATTTCAAA+GGG - chr1.2:10219841-10219860 None:intergenic 25.0%
! ATAATTGGAATGTAATGAAC+TGG + chr1.2:10218980-10218999 MS.gene54077:intron 25.0%
! ATGAAATACTGCTTAATTTC+AGG + chr1.2:10221965-10221984 MS.gene54077:intron 25.0%
! TAGTAAAAATAATTTCACCG+AGG - chr1.2:10219238-10219257 None:intergenic 25.0%
! TATATCAAACCCTAATTTCT+TGG + chr1.2:10218381-10218400 MS.gene54077:intron 25.0%
! TGTGTTATAATACAATCATG+TGG + chr1.2:10219621-10219640 MS.gene54077:intron 25.0%
! TTTATATGATATAAGCTCGT+TGG + chr1.2:10220038-10220057 MS.gene54077:intron 25.0%
!! CAAAATGTTTAAAAGGCTTT+TGG - chr1.2:10221339-10221358 None:intergenic 25.0%
!! GATTGAAATCTTTTGTTGAT+AGG + chr1.2:10218739-10218758 MS.gene54077:intron 25.0%
!! GTTGATGTTTGGAATATAAA+TGG + chr1.2:10218797-10218816 MS.gene54077:intron 25.0%
!!! CTGATTATAATGTTGCTTTT+TGG + chr1.2:10219191-10219210 MS.gene54077:intron 25.0%
!!! TCTTCTTATTTGTTTTTCAG+TGG + chr1.2:10219708-10219727 MS.gene54077:intron 25.0%
!!! TTTGCTTCTTTTGATTAATG+TGG + chr1.2:10219122-10219141 MS.gene54077:intron 25.0%
!!! TTTGGTGGTAATTTTTTATG+TGG + chr1.2:10218904-10218923 MS.gene54077:intron 25.0%
!!! TTTGTTGATAGGTTATGTTT+TGG + chr1.2:10218750-10218769 MS.gene54077:intron 25.0%
AATGAAATCATCCATTGAAG+AGG - chr1.2:10221806-10221825 None:intergenic 30.0%
ACATTGTGAAGATTTCAAAG+GGG - chr1.2:10219840-10219859 None:intergenic 30.0%
CAACATTGTGAAGATTTCAA+AGG - chr1.2:10219842-10219861 None:intergenic 30.0%
CGGTTAATTCCAAGAAATTA+GGG - chr1.2:10218393-10218412 None:intergenic 30.0%
GACATAAACAAAATAGCAGA+AGG - chr1.2:10219504-10219523 None:intergenic 30.0%
TCCTTAAAAATTTACAGCCA+AGG + chr1.2:10221530-10221549 MS.gene54077:intron 30.0%
TCGGTTAATTCCAAGAAATT+AGG - chr1.2:10218394-10218413 None:intergenic 30.0%
TTCTTGGAATTAACCGAAAA+TGG + chr1.2:10218397-10218416 MS.gene54077:intron 30.0%
! AAAGCTTAGAATACCTTTTC+AGG - chr1.2:10219457-10219476 None:intergenic 30.0%
! AACAGAGTATTAAGACAACA+AGG - chr1.2:10221475-10221494 None:intergenic 30.0%
! AAGCTTAGAATACCTTTTCA+GGG - chr1.2:10219456-10219475 None:intergenic 30.0%
! ATGATTTGCATTGAGTACTA+AGG - chr1.2:10219360-10219379 None:intergenic 30.0%
! GATTAGTTAGAAGTAGAACT+AGG + chr1.2:10219653-10219672 MS.gene54077:intron 30.0%
! GCTTTTGATAGATTCTTGTT+TGG + chr1.2:10218886-10218905 MS.gene54077:intron 30.0%
! TATACAGAATCCACCATTTT+CGG - chr1.2:10218413-10218432 None:intergenic 30.0%
! TCCTTGGCTGTAAATTTTTA+AGG - chr1.2:10221534-10221553 None:intergenic 30.0%
! TGTTGCTTCATTATATACCT+CGG + chr1.2:10219218-10219237 MS.gene54077:intron 30.0%
! TTAGCTTATGTGTTGATGTT+TGG + chr1.2:10218786-10218805 MS.gene54077:intron 30.0%
! TTTCTTTTTGAACAACGAGA+AGG + chr1.2:10221414-10221433 MS.gene54077:intron 30.0%
! TTTGATAGATTCTTGTTTGG+TGG + chr1.2:10218889-10218908 MS.gene54077:intron 30.0%
! TTTTTATGTGGATAGTTTGG+TGG + chr1.2:10218916-10218935 MS.gene54077:intron 30.0%
!! AATGACTTTCTAAAGCAATG+AGG + chr1.2:10219085-10219104 MS.gene54077:intron 30.0%
!! CTTTGAAATCTTCACAATGT+TGG + chr1.2:10219840-10219859 MS.gene54077:intron 30.0%
!! TCTTATTTGTTTTTCAGTGG+AGG + chr1.2:10219711-10219730 MS.gene54077:intron 30.0%
!!! TTTCAATCCATTTTTTCGGT+TGG - chr1.2:10218656-10218675 None:intergenic 30.0%
AAAAATTTACAGCCAAGGAG+AGG + chr1.2:10221535-10221554 MS.gene54077:intron 35.0%
AAAGCAAGAAAAGGGCAAAA+CGG - chr1.2:10218298-10218317 None:intergenic 35.0%
ACCCTTACCTAGTATTTGTT+TGG - chr1.2:10219915-10219934 None:intergenic 35.0%
AGGCTGAGATCTTAATACAA+TGG - chr1.2:10221786-10221805 None:intergenic 35.0%
ATATGATATAAGCTCGTTGG+AGG + chr1.2:10220041-10220060 MS.gene54077:intron 35.0%
ATATGCATGTGTAATGTGTG+TGG - chr1.2:10220019-10220038 None:intergenic 35.0%
ATCTGTACGAAAGCAAGAAA+AGG - chr1.2:10218307-10218326 None:intergenic 35.0%
ATTTCCCCCAAACAAATACT+AGG + chr1.2:10219905-10219924 MS.gene54077:intron 35.0%
CATTGTGAAGATTTCAAAGG+GGG - chr1.2:10219839-10219858 None:intergenic 35.0%
CCAAAAGGCTAATGAATGTA+TGG - chr1.2:10221588-10221607 None:intergenic 35.0%
CCCAAACAAATACTAGGTAA+GGG + chr1.2:10219911-10219930 MS.gene54077:intron 35.0%
CCCTTACCTAGTATTTGTTT+GGG - chr1.2:10219914-10219933 None:intergenic 35.0%
CCTTACCTAGTATTTGTTTG+GGG - chr1.2:10219913-10219932 None:intergenic 35.0%
CTCAATGCAAATCATTCTTG+AGG + chr1.2:10219365-10219384 MS.gene54077:CDS 35.0%
CTTACCTAGTATTTGTTTGG+GGG - chr1.2:10219912-10219931 None:intergenic 35.0%
GAAATCTTCACAATGTTGGA+GGG + chr1.2:10219844-10219863 MS.gene54077:intron 35.0%
GTATCATTTAGATTGCCAGT+AGG - chr1.2:10221895-10221914 None:intergenic 35.0%
TAGTATTTGTTTGGGGGAAA+TGG - chr1.2:10219906-10219925 None:intergenic 35.0%
TCAAACTTCTGCTTCACTTT+AGG + chr1.2:10221228-10221247 MS.gene54077:intron 35.0%
TCTGTACGAAAGCAAGAAAA+GGG - chr1.2:10218306-10218325 None:intergenic 35.0%
TGAAATCTTCACAATGTTGG+AGG + chr1.2:10219843-10219862 MS.gene54077:intron 35.0%
TGACATTCAGAAGAAATCTC+CGG + chr1.2:10218016-10218035 MS.gene54077:CDS 35.0%
TTAGAAGTAGAACTAGGAAG+AGG + chr1.2:10219659-10219678 MS.gene54077:intron 35.0%
TTATCAACTGTGACGATTGT+TGG + chr1.2:10221373-10221392 MS.gene54077:intron 35.0%
TTGGAATTAACCGAAAATGG+TGG + chr1.2:10218400-10218419 MS.gene54077:intron 35.0%
! TTTGGAACCAACCGAAAAAA+TGG + chr1.2:10218646-10218665 MS.gene54077:intron 35.0%
!! AAAGGCTTTTGGTTGAGTTT+AGG - chr1.2:10221328-10221347 None:intergenic 35.0%
!! GTTGATAGGTTATGTTTTGG+TGG + chr1.2:10218753-10218772 MS.gene54077:intron 35.0%
!! GTTGCTGATTTATCAAATGC+TGG + chr1.2:10222050-10222069 MS.gene54077:CDS 35.0%
!!! CCATACATTCATTAGCCTTT+TGG + chr1.2:10221585-10221604 MS.gene54077:CDS 35.0%
AAAGCTGAACCGAAGGATAA+GGG + chr1.2:10218068-10218087 MS.gene54077:CDS 40.0%
AACAATATCTGCGATGACCT+TGG - chr1.2:10222033-10222052 None:intergenic 40.0%
AAGTAGAACTAGGAAGAGGA+AGG + chr1.2:10219663-10219682 MS.gene54077:intron 40.0%
ACAAAGATGATGACCCTAAC+AGG + chr1.2:10218222-10218241 MS.gene54077:CDS 40.0%
AGGGTTTGTGTACAACTTAC+TGG + chr1.2:10219930-10219949 MS.gene54077:intron 40.0%
ATTTCAAAGCAACCTCTCCT+TGG - chr1.2:10221550-10221569 None:intergenic 40.0%
CCCCAAACAAATACTAGGTA+AGG + chr1.2:10219910-10219929 MS.gene54077:intron 40.0%
GCTTTCTTTCCCTTATCCTT+CGG - chr1.2:10218080-10218099 None:intergenic 40.0%
GGAAAAAATCTTCGTTCCTC+CGG + chr1.2:10217945-10217964 MS.gene54077:CDS 40.0%
GGGTTTGTGTACAACTTACT+GGG + chr1.2:10219931-10219950 MS.gene54077:intron 40.0%
GGTTTGTGTACAACTTACTG+GGG + chr1.2:10219932-10219951 MS.gene54077:intron 40.0%
GTACAGATGCACGATAATGA+CGG + chr1.2:10218319-10218338 MS.gene54077:intron 40.0%
GTTTGTGTACAACTTACTGG+GGG + chr1.2:10219933-10219952 MS.gene54077:intron 40.0%
TAAGATCTCAGCCTCTTCAA+TGG + chr1.2:10221792-10221811 MS.gene54077:intron 40.0%
TAGTCAAGCTATTGATGTGG+TGG + chr1.2:10219420-10219439 MS.gene54077:CDS 40.0%
! AAAATAGCAGAAGGCATGAG+TGG - chr1.2:10219495-10219514 None:intergenic 40.0%
! AGAGAGCATGACTTACCAAA+AGG - chr1.2:10221603-10221622 None:intergenic 40.0%
! CGGTTGGTTCCAAAAAAGTA+GGG - chr1.2:10218640-10218659 None:intergenic 40.0%
! TCGGTTGGTTCCAAAAAAGT+AGG - chr1.2:10218641-10218660 None:intergenic 40.0%
! TGGTTGAGTTTAGGTTTGCA+GGG - chr1.2:10221319-10221338 None:intergenic 40.0%
! TTGGTTGAGTTTAGGTTTGC+AGG - chr1.2:10221320-10221339 None:intergenic 40.0%
!! AGATCCTTCATTGGCTTGAA+TGG - chr1.2:10222002-10222021 None:intergenic 40.0%
!!! GAACGAAGATTTTTTCCTCC+CGG - chr1.2:10217942-10217961 None:intergenic 40.0%
!!! GAGACGAAACCCTACTTTTT+TGG + chr1.2:10218628-10218647 MS.gene54077:intron 40.0%
AACTAGGAAGAGGAAGGAGA+GGG + chr1.2:10219669-10219688 MS.gene54077:intron 45.0%
ACGACGATAACAGTCCTGTT+AGG - chr1.2:10218239-10218258 None:intergenic 45.0%
ACTGTGACGATTGTTGGTCA+TGG + chr1.2:10221379-10221398 MS.gene54077:intron 45.0%
AGCAGAGAGAAAGTAGGAAG+AGG - chr1.2:10221277-10221296 None:intergenic 45.0%
AGTGGAAGCAGAGAGAAAGT+AGG - chr1.2:10221283-10221302 None:intergenic 45.0%
ATTCTTGAGGAGAGCTACTG+AGG + chr1.2:10219378-10219397 MS.gene54077:CDS 45.0%
CAGGGTGAGAGTCAAAATAG+TGG - chr1.2:10221301-10221320 None:intergenic 45.0%
CGACGATAACAGTCCTGTTA+GGG - chr1.2:10218238-10218257 None:intergenic 45.0%
CTGTTATCGTCGTTGAGCAT+TGG + chr1.2:10218246-10218265 MS.gene54077:CDS 45.0%
GAAAGCTGAACCGAAGGATA+AGG + chr1.2:10218067-10218086 MS.gene54077:CDS 45.0%
GAAGAAGAAAGCTGAACCGA+AGG + chr1.2:10218061-10218080 MS.gene54077:CDS 45.0%
GCAGAGAGAAAGTAGGAAGA+GGG - chr1.2:10221276-10221295 None:intergenic 45.0%
GCCAATGAAGGATCTTGACA+TGG + chr1.2:10222007-10222026 MS.gene54077:CDS 45.0%
GCCATGTCAAGATCCTTCAT+TGG - chr1.2:10222011-10222030 None:intergenic 45.0%
GGGTAGTCAAGCTATTGATG+TGG + chr1.2:10219417-10219436 MS.gene54077:CDS 45.0%
GGGTCATCATCTTTGTCTTC+AGG - chr1.2:10218218-10218237 None:intergenic 45.0%
GGTGAAACTGAACCCTGAAA+AGG + chr1.2:10219441-10219460 MS.gene54077:CDS 45.0%
TCGTCCATTCAAGCCAATGA+AGG + chr1.2:10221995-10222014 MS.gene54077:CDS 45.0%
TGTTGGAGGGAGCTAATGTT+TGG + chr1.2:10219857-10219876 MS.gene54077:intron 45.0%
TTCTTCTGCTTCTTCTTCGC+CGG - chr1.2:10218038-10218057 None:intergenic 45.0%
! GTTGGAACGAAGTGTTCTTC+CGG - chr1.2:10217967-10217986 None:intergenic 45.0%
! TTTGAAATACGCCAGCAAGG+TGG + chr1.2:10221562-10221581 MS.gene54077:CDS 45.0%
!! CTTGTTTTCCCTTACCCTTC+GGG - chr1.2:10218154-10218173 None:intergenic 45.0%
!! GCTTGTTTTCCCTTACCCTT+CGG - chr1.2:10218155-10218174 None:intergenic 45.0%
!! TGCTTTGAAATACGCCAGCA+AGG + chr1.2:10221559-10221578 MS.gene54077:CDS 45.0%
AAACAAGCCGCTGTACCTGA+AGG + chr1.2:10218167-10218186 MS.gene54077:CDS 50.0%
AAGAAAGCCGCTGACAGTGA+AGG + chr1.2:10218092-10218111 MS.gene54077:CDS 50.0%
ACACTTCGTTCCAACTCCGA+AGG + chr1.2:10217972-10217991 MS.gene54077:CDS 50.0%
ACTAGGAAGAGGAAGGAGAG+GGG + chr1.2:10219670-10219689 MS.gene54077:intron 50.0%
AGAGCTACTGAGGTGAAGGA+TGG + chr1.2:10219388-10219407 MS.gene54077:CDS 50.0%
CAAGGCTAAAACCGCTTCTC+CGG + chr1.2:10217896-10217915 MS.gene54077:CDS 50.0%
GAACTAGGAAGAGGAAGGAG+AGG + chr1.2:10219668-10219687 MS.gene54077:intron 50.0%
GAAGGATCTTGACATGGCCA+AGG + chr1.2:10222013-10222032 MS.gene54077:CDS 50.0%
GGCGAAGAAGAAGCAGAAGA+AGG + chr1.2:10218037-10218056 MS.gene54077:CDS 50.0%
TACTGAGGTGAAGGATGGTC+TGG + chr1.2:10219393-10219412 MS.gene54077:CDS 50.0%
TGAATGTATGGCCACCTTGC+TGG - chr1.2:10221576-10221595 None:intergenic 50.0%
!! AGAAGCACCTTCACTGTCAG+CGG - chr1.2:10218102-10218121 None:intergenic 50.0%
!! GAAGGTGCTTCTGGTACTAC+TGG + chr1.2:10218110-10218129 MS.gene54077:CDS 50.0%
!! GCTTCTGGTACTACTGGTTC+TGG + chr1.2:10218116-10218135 MS.gene54077:CDS 50.0%
!! TCTGGTACTACTGGTTCTGG+TGG + chr1.2:10218119-10218138 MS.gene54077:CDS 50.0%
!!! AGATTTTTTCCTCCCGGCGA+CGG - chr1.2:10217936-10217955 None:intergenic 50.0%
!!! GCGGTTTTAGCCTTGCGTTT+TGG - chr1.2:10217891-10217910 None:intergenic 50.0%
AAGCCTTGCACCTTCGGAGT+TGG - chr1.2:10217985-10218004 None:intergenic 55.0%
ACCTCCACCTTCAGGTACAG+CGG - chr1.2:10218177-10218196 None:intergenic 55.0%
CGAGACAAGCCTTGCACCTT+CGG - chr1.2:10217991-10218010 None:intergenic 55.0%
CTAGGAAGAGGAAGGAGAGG+GGG + chr1.2:10219671-10219690 MS.gene54077:intron 55.0%
GAGGAGAGCTACTGAGGTGA+AGG + chr1.2:10219384-10219403 MS.gene54077:CDS 55.0%
GAGGTGAAGGATGGTCTGGA+GGG + chr1.2:10219397-10219416 MS.gene54077:CDS 55.0%
GCATCAGAACCTCCACCTTC+AGG - chr1.2:10218185-10218204 None:intergenic 55.0%
TAAAACCGCTTCTCCGGCGA+AGG + chr1.2:10217902-10217921 MS.gene54077:CDS 55.0%
TAGGAAGAGGAAGGAGAGGG+GGG + chr1.2:10219672-10219691 MS.gene54077:intron 55.0%
TGAGGTGAAGGATGGTCTGG+AGG + chr1.2:10219396-10219415 MS.gene54077:CDS 55.0%
! GGAACGAAGTGTTCTTCCGG+AGG - chr1.2:10217964-10217983 None:intergenic 55.0%
! GTTCCAACTCCGAAGGTGCA+AGG + chr1.2:10217979-10217998 MS.gene54077:CDS 55.0%
!! GCTGACAGTGAAGGTGCTTC+TGG + chr1.2:10218101-10218120 MS.gene54077:CDS 55.0%
!! GGTACTACTGGTTCTGGTGG+TGG + chr1.2:10218122-10218141 MS.gene54077:CDS 55.0%
AAGAGGAAGGAGAGGGGGGT+TGG + chr1.2:10219676-10219695 MS.gene54077:intron 60.0%
CAAGCCGCTGTACCTGAAGG+TGG + chr1.2:10218170-10218189 MS.gene54077:CDS 60.0%
!! GGTGCTGACCCGAAGGGTAA+GGG + chr1.2:10218143-10218162 MS.gene54077:CDS 60.0%
!! TGGTGCTGACCCGAAGGGTA+AGG + chr1.2:10218142-10218161 MS.gene54077:CDS 60.0%
GCCGCTGTACCTGAAGGTGG+AGG + chr1.2:10218173-10218192 MS.gene54077:CDS 65.0%
!! GGTGGTGGTGCTGACCCGAA+GGG + chr1.2:10218137-10218156 MS.gene54077:CDS 65.0%
!! TGGTGGTGGTGCTGACCCGA+AGG + chr1.2:10218136-10218155 MS.gene54077:CDS 65.0%
CGCAGCCTTCGCCGGAGAAG+CGG - chr1.2:10217910-10217929 None:intergenic 70.0%
CGCTTCTCCGGCGAAGGCTG+CGG + chr1.2:10217908-10217927 MS.gene54077:CDS 70.0%
! AAGGCTGCGGCGCCGTCGCC+GGG + chr1.2:10217921-10217940 MS.gene54077:CDS 80.0%
! GAAGGCTGCGGCGCCGTCGC+CGG + chr1.2:10217920-10217939 MS.gene54077:CDS 80.0%
! GACGGCGCCGCAGCCTTCGC+CGG - chr1.2:10217918-10217937 None:intergenic 80.0%
! GCTGCGGCGCCGTCGCCGGG+AGG + chr1.2:10217924-10217943 MS.gene54077:CDS 90.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 10217882 10222076 10217882 ID=MS.gene54077
chr1.2 mRNA 10217882 10222076 10217882 ID=MS.gene54077.t1;Parent=MS.gene54077
chr1.2 exon 10217882 10218267 10217882 ID=MS.gene54077.t1.exon1;Parent=MS.gene54077.t1
chr1.2 CDS 10217882 10218267 10217882 ID=cds.MS.gene54077.t1;Parent=MS.gene54077.t1
chr1.2 exon 10219363 10219462 10219363 ID=MS.gene54077.t1.exon2;Parent=MS.gene54077.t1
chr1.2 CDS 10219363 10219462 10219363 ID=cds.MS.gene54077.t1;Parent=MS.gene54077.t1
chr1.2 exon 10221547 10221606 10221547 ID=MS.gene54077.t1.exon3;Parent=MS.gene54077.t1
chr1.2 CDS 10221547 10221606 10221547 ID=cds.MS.gene54077.t1;Parent=MS.gene54077.t1
chr1.2 exon 10221987 10222076 10221987 ID=MS.gene54077.t1.exon4;Parent=MS.gene54077.t1
chr1.2 CDS 10221987 10222076 10221987 ID=cds.MS.gene54077.t1;Parent=MS.gene54077.t1
Gene Sequence

>MS.gene54077

ATGGCACCAAAACGCAAGGCTAAAACCGCTTCTCCGGCGAAGGCTGCGGCGCCGTCGCCGGGAGGAAAAAATCTTCGTTCCTCCGGAAGAACACTTCGTTCCAACTCCGAAGGTGCAAGGCTTGTCTCGTTAGATGACATTCAGAAGAAATCTCCGGCGAAGAAGAAGCAGAAGAAGGAGAAGAAGAAAGCTGAACCGAAGGATAAGGGAAAGAAAGCCGCTGACAGTGAAGGTGCTTCTGGTACTACTGGTTCTGGTGGTGGTGCTGACCCGAAGGGTAAGGGAAAACAAGCCGCTGTACCTGAAGGTGGAGGTTCTGATGCTGCTGATAAACCTGAAGACAAAGATGATGACCCTAACAGGACTGTTATCGTCGTTGAGCATTGGTATTTCACTCTTTCGCTTTTTTTTTACCGTTTTGCCCTTTTCTTGCTTTCGTACAGATGCACGATAATGACGGAGAAATTATTATTCGATTTTGTTTCGTTAATGCAAATTTTATATCAAACCCTAATTTCTTGGAATTAACCGAAAATGGTGGATTCTGTATAAGCTATTTTTAATCGTTTTCGCTTTTCTTGCTTTCGTAATAATGTCCGTAATGATGACGAAAAATATTATGCGCTTTTTCAGTTTTTTTATTTTTATTTTATTTGTATGCATGTGTTTTGTGTTTGTCTTTATCTTTATCTGCTTATTTTGTTTTTATATTAGCTTAATCAATATGAAAACGATTTATTTTTTGTGAGACGAAACCCTACTTTTTTGGAACCAACCGAAAAAATGGATTGAAAAATTTGTTGATTAAATTGAATCTTTTGTTGATTAAAGTGTAATAAATGGAATATATTGAAATGGATTGAAATCTTTTGTTGATAGGTTATGTTTTGGTGGTAAATCATGATTAGCTTATGTGTTGATGTTTGGAATATAAATGGAATATAGATAATGAAATGGATTGAATAAATTGTTGATTAAAGTGAATCTTTTGTTGATAAATTGAAGCTTTTGATAGATTCTTGTTTGGTGGTAATTTTTTATGTGGATAGTTTGGTGGTTAAATTTGATAGTATGTGTTTATATATTGAATAGAGTGTAATAATTGGAATGTAATGAACTGGATTGAAAAAGAGTGTCATTTTTTTGATGATTAATTGATGCAGATTGTTGAAATATTAATGATAATATTTCTTGATTGATTATAATGACTTTCTAAAGCAATGAGGAGCCTGAAAAATAATTTGCTTCTTTTGATTAATGTGGAATTTTGAGATAATTACAAATAATCTTTTTAACAAGATTCTTGAATCTGATTATAATGTTGCTTTTTGGTTCTTGTTGCTTCATTATATACCTCGGTGAAATTATTTTTACTATTATTGATTTCTCTCTCTAACATTGTAACTGTTTTCTCAATTGAGTGACTGATTATTGTTTTACTATTATTGATTTCTCAATTTTTTTGTCTTGATTTTCCTTAGTACTCAATGCAAATCATTCTTGAGGAGAGCTACTGAGGTGAAGGATGGTCTGGAGGGTAGTCAAGCTATTGATGTGGTGGTGAAACTGAACCCTGAAAAGGTATTCTAAGCTTTATTTATCATTTCTATCCACTCATGCCTTCTGCTATTTTGTTTATGTCAATGATATCAAATAGATATACATGTTTTTCAAAAAAACATTGATTATAATACAATCAATGTTTATTCTGCCATTGATTATTTTACCACTTTACATTATGTGTTATAATACAATCATGTGGTGTTTGAATGATTAGTTAGAAGTAGAACTAGGAAGAGGAAGGAGAGGGGGGTTGGTCAATAGAATCTTCTTATTTGTTTTTCAGTGGAGGTGTGATTTTAATAATGAAAACACTAATTCAAAATTTACTCTAAGTTTATGTATTTAAAATATTTATGCAATTCATGTTCACTGTTTTATTCATCTCTGATCTCCCCCTTTGAAATCTTCACAATGTTGGAGGGAGCTAATGTTTGGTGAAGAGAAAAATCTCTCTATATCCATTTCCCCCAAACAAATACTAGGTAAGGGTTTGTGTACAACTTACTGGGGGAGTTTTTGAAAATAAGTTGACAACTTATTGACATATTATATATTATTTTTAAAAGTTCTTCCACACACATTACACATGCATATTTATATGATATAAGCTCGTTGGAGGATGTATTTAAAATATTTATGCAATTCATGTTCACTGTTTTATTCATCTCTGATCTCCCCCTTTGAAATCTTCACAATGTTGGAGGGAGCTAATGTTTGGTGAAGAGAGATTTTTCATATCTCTCTATATCCATTTCCCCCAAACAAATACTAGGTAAGGGTTTGTGTACAACTTACTGGGGGAGTTTTTGAAAATAAGTTGACAACTTATTGACATATTATATATTATTTTTAAAAGTTCTTCCACACACATTACACATGCATATTTATATGATATAAGCTCGTTGGAGGATGTATTTAAAATATTTATGCAATTCATGTTCACTGTTTTATTCATCTCTGATCTCCCCCTTTGAAATCTTCACAATGTTGGAGGGAGCTAATGTTTGGTGAAGAGAGATTTTTCATATCTCTCTATATCCATTTCCCCCAAACAAATACTAGGTAAGGGTTTGTGTACAACTTACTGGGGGAGTTTTTGAAAATAAGTTGACAACTTATTGACATATTATATATTATTTTTAAAAGTTCTTCCACACACATTACACATGCATATTTATATGATATAAGCTCGTTGGAGGATGTACTTAAAATATTTATGCAATTCATGTTCACTGTTTTATTCATCTCTGATCCCCCTTTGAAATCTTCACAATGTTGGAGGGAGCTAATGTTTGGTGAAGAGAGATTTTTCATATCTCTCTATATCCATTTCCCCCAAACAAATACTAGGTAAGGGTTTGTGTACAACTTACTGGGGGAGTTTTTGAAAATAAGTTGACAACTTATTGACATATTATATATTATTTTTAAAAGTTCTTCCACACACATTACACATGCATATTTATATGATATAAGCTCGTTGGAGGATGTACTTAAAATATTTATGCAATTCATGTTCACTGTTTTATTCATCTCTGATCTCCCCCTTTGAAATCTTCACAATGTTGGAGGGAGCTAATGTTTGGTGAAGAGAGATTTTTCATATCTCTCTATATCCATTTCCCCCAAACAAATACTAGGTAAGGGTTTGTGTACAACTTACTGGGGGAGTTTTTGAAAATAAGTTGACAACTTATTGACATCTTATATATTATTTTTAAAAGTTCTTCCACACACATTACACATGCATATTTATATGATATAAGCTCTCAAATAAGCTCTTTCAAACTTCTGCTTCACTTTAGGTGACATCTCTACTAAACTCTCACCCTCTTCCTACTTTCTCTCTGCTTCCACTATTTTGACTCTCACCCTGCAAACCTAAACTCAACCAAAAGCCTTTTAAACATTTTGTTTTCAATGATAGCTTATCAACTGTGACGATTGTTGGTCATGGCCTTTTTTACAATTTCTTTTTGAACAACGAGAAGGACTATTGAATTCATATGCTATATTATTTAACTAGTCCTTGTTGTCTTAATACTCTGTTTTTTATGTCAACAATACTTGATTGATCCAATTTTTTCCTTAAAAATTTACAGCCAAGGAGAGGTTGCTTTGAAATACGCCAGCAAGGTGGCCATACATTCATTAGCCTTTTGGTAAGTCATGCTCTCTTGTTATCAACATGAACATTTTTTAATTGGCAAGCTAAATCAACCTTTGTACTTTTTTCTATTTGTTCATATGCTATATTTGAAGTTTTTTTGGATATATTGGCCATTATTTTTTAGTCATTTTTCTTGCTCAAGAAAGTATTAAGATTTTTTAGATTGTACCATTGTATTAAGATCTCAGCCTCTTCAATGGATGATTTCATTCACATGAAAATATTTCTTTTCTGTATGTTTTAAAGAACTTCAATTTCTTTATTAGTTTAAAGGCATCCTACTGGCAATCTAAATGATACATGCATACATTTAATTATATTTTTATGATATTCTGTTTTAATACTAACTTATGAAATACTGCTTAATTTCAGGACATAAGTCGTCCATTCAAGCCAATGAAGGATCTTGACATGGCCAAGGTCATCGCAGATATTGTTGCTGATTTATCAAATGCTGGTTGA
Protein sequence

>MS.gene54077.t1

MAPKRKAKTASPAKAAAPSPGGKNLRSSGRTLRSNSEGARLVSLDDIQKKSPAKKKQKKEKKKAEPKDKGKKAADSEGASGTTGSGGGADPKGKGKQAAVPEGGGSDAADKPEDKDDDPNRTVIVVEHCTQCKSFLRRATEVKDGLEGSQAIDVVVKLNPEKPRRGCFEIRQQGGHTFISLLDISRPFKPMKDLDMAKVIADIVADLSNAG