AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058818


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058818.t1 MTR_8g086640 98.000 150 3 0 1 150 1 150 3.92e-101 286
MS.gene058818.t1 MTR_4g063280 95.455 132 5 1 20 150 14 145 1.59e-84 244
MS.gene058818.t1 MTR_2g096610 87.333 150 17 1 1 150 1 148 1.63e-83 242
MS.gene058818.t1 MTR_4g064967 94.000 150 7 1 1 150 1 148 2.88e-81 236
MS.gene058818.t1 MTR_4g071150 91.503 153 9 2 1 150 1 152 8.92e-80 233
MS.gene058818.t1 MTR_4g064005 91.333 150 11 1 1 150 1 148 4.09e-79 231
MS.gene058818.t1 MTR_4g063410 90.667 150 12 1 1 150 1 148 9.41e-79 230
MS.gene058818.t1 MTR_2g096570 89.610 154 11 3 1 150 1 153 6.78e-78 228
MS.gene058818.t1 MTR_7g113160 81.955 133 23 1 10 142 13 144 2.68e-72 213
MS.gene058818.t1 MTR_7g108320 70.213 141 39 2 3 142 2 140 1.69e-60 183
MS.gene058818.t1 MTR_7g114060 70.000 140 38 1 3 142 2 137 7.19e-60 182
MS.gene058818.t1 MTR_7g114040 70.000 140 38 1 3 142 2 137 7.19e-60 182
MS.gene058818.t1 MTR_2g082510 74.790 119 30 0 11 129 3 121 1.62e-58 178
MS.gene058818.t1 MTR_7g093910 72.222 126 32 1 4 129 5 127 2.60e-58 178
MS.gene058818.t1 MTR_2g082220 73.950 119 31 0 11 129 3 121 2.40e-57 175
MS.gene058818.t1 MTR_6g005150 64.567 127 31 3 24 149 26 139 6.55e-48 151
MS.gene058818.t1 MTR_7g114050 65.385 130 30 3 17 142 10 128 2.08e-46 147
MS.gene058818.t1 MTR_4g063300 76.190 105 20 1 45 149 10 109 7.34e-46 145
MS.gene058818.t1 MTR_1g011750 59.292 113 44 2 21 132 23 134 5.99e-36 121
MS.gene058818.t1 MTR_1g011850 59.292 113 44 2 21 132 23 134 5.99e-36 121
MS.gene058818.t1 MTR_1g114050 59.633 109 42 2 21 128 20 127 5.83e-35 118
MS.gene058818.t1 MTR_1g071040 61.538 104 38 2 26 128 28 130 2.86e-34 117
MS.gene058818.t1 MTR_1g069770 53.333 90 41 1 45 133 9 98 1.46e-24 91.3
MS.gene058818.t1 MTR_7g010440 70.588 51 15 0 78 128 122 172 3.09e-16 73.9
MS.gene058818.t1 MTR_4g048490 43.810 105 30 2 24 128 26 101 3.42e-16 70.1
MS.gene058818.t1 MTR_4g005910 74.419 43 11 0 87 129 4 46 5.10e-16 68.2
MS.gene058818.t1 MTR_7g027610 40.517 116 43 3 20 134 10 100 3.12e-15 67.8
MS.gene058818.t1 MTR_2g096590 72.500 40 11 0 110 149 12 51 3.03e-12 58.2
MS.gene058818.t1 MTR_0653s0030 53.704 54 25 0 56 109 44 97 1.38e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058818.t1 AT5G02560 82.353 153 24 1 1 150 1 153 2.22e-80 234
MS.gene058818.t1 AT5G02560 71.186 177 24 2 1 150 1 177 2.21e-75 223
MS.gene058818.t1 AT5G27670 75.000 152 34 3 1 150 1 150 1.39e-72 214
MS.gene058818.t1 AT5G59870 75.000 148 36 1 4 150 3 150 8.98e-72 212
MS.gene058818.t1 AT1G51060 76.667 120 28 0 11 130 3 122 9.15e-61 184
MS.gene058818.t1 AT4G27230 75.630 119 29 0 11 129 3 121 1.76e-59 181
MS.gene058818.t1 AT4G27230 75.630 119 29 0 11 129 3 121 1.76e-59 181
MS.gene058818.t1 AT3G20670 75.630 119 29 0 11 129 3 121 7.39e-59 179
MS.gene058818.t1 AT1G08880 71.318 129 34 1 7 132 2 130 1.84e-58 178
MS.gene058818.t1 AT5G54640 74.790 119 30 0 11 129 3 121 4.41e-58 177
MS.gene058818.t1 AT1G54690 72.800 125 31 1 11 132 6 130 8.23e-58 177
MS.gene058818.t1 AT2G38810 59.821 112 43 2 21 131 25 135 1.96e-36 122
MS.gene058818.t1 AT2G38810 59.821 112 43 2 21 131 25 135 1.96e-36 122
MS.gene058818.t1 AT2G38810 59.821 112 43 2 21 131 25 135 1.96e-36 122
MS.gene058818.t1 AT2G38810 59.821 112 43 2 21 131 25 135 1.96e-36 122
MS.gene058818.t1 AT1G52740 59.633 109 42 2 25 132 27 134 1.08e-34 118
MS.gene058818.t1 AT3G54560 58.716 109 43 2 25 132 29 136 1.73e-34 117
MS.gene058818.t1 AT3G54560 58.716 109 43 2 25 132 29 136 1.73e-34 117
MS.gene058818.t1 AT4G13570 47.826 92 46 2 32 122 28 118 8.57e-20 79.7
MS.gene058818.t1 AT4G13570 47.253 91 46 2 33 122 35 124 2.67e-19 78.6

Find 43 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGCCTTAGAAGGAGATTT+AGG 0.266583 4.1:-13476117 None:intergenic
AAACTAATCTAAGCCTTCTT+AGG 0.296900 4.1:-13476147 None:intergenic
AAAAGAACAGGGTTAATATT+AGG 0.325842 4.1:-13476063 None:intergenic
TGCTGCTGAAGTTCTTGAAT+TGG 0.339223 4.1:+13475912 MS.gene058818:CDS
CAATTCCCAGTCGGAAGAAT+TGG 0.342053 4.1:+13475808 MS.gene058818:CDS
CTTGTTACCGACTTCTTCCT+TGG 0.365854 4.1:-13475766 None:intergenic
GGAGCCTAAATCTCCTTCTA+AGG 0.376066 4.1:+13476113 MS.gene058818:CDS
GAAAACTGAGAAAGCTGTTA+AGG 0.380382 4.1:+13476092 MS.gene058818:CDS
GAGCTTGGGAAATTGCTTAC+TGG 0.397564 4.1:+13476012 MS.gene058818:CDS
AGAACTGCGGCTAGGTAAAC+AGG 0.399219 4.1:-13475880 None:intergenic
AGCACAAAGGCGACGAAGAA+AGG 0.403145 4.1:+13475718 MS.gene058818:CDS
GCTGAAGTTCTTGAATTGGC+TGG 0.461152 4.1:+13475916 MS.gene058818:CDS
TCCGACTGGGAATTGAAGTC+CGG 0.472609 4.1:-13475800 None:intergenic
GAAGGAAAGGCGGAGGACCA+AGG 0.493860 4.1:+13475749 MS.gene058818:CDS
TATACCAAGGCATGTGTTGT+TGG 0.495755 4.1:+13475972 MS.gene058818:CDS
ATTGGCCGTTACTTGAAGAA+AGG 0.513109 4.1:+13475826 MS.gene058818:CDS
TAACGGCCAATTCTTCCGAC+TGG 0.515556 4.1:-13475814 None:intergenic
AAAGGAGCTGGAGGAAGGAA+AGG 0.517528 4.1:+13475736 MS.gene058818:CDS
CACGTGATAACAAGAAGAAT+CGG 0.520472 4.1:+13475947 MS.gene058818:CDS
CGAAGAAAGGAGCTGGAGGA+AGG 0.526335 4.1:+13475731 MS.gene058818:CDS
GCTGGAGGAAGGAAAGGCGG+AGG 0.531971 4.1:+13475742 MS.gene058818:CDS
TACGCTCAACGTGTTGGCAC+CGG 0.537157 4.1:+13475853 MS.gene058818:CDS
AAGGCGACGAAGAAAGGAGC+TGG 0.543027 4.1:+13475724 MS.gene058818:CDS
GTGAGGAATGATGAAGAGCT+TGG 0.543487 4.1:+13475997 MS.gene058818:CDS
ACTGGTGTTACCATTGCTCA+TGG 0.545887 4.1:+13476030 MS.gene058818:CDS
ACAGCCAACAACACATGCCT+TGG 0.551257 4.1:-13475976 None:intergenic
TGAGGAATGATGAAGAGCTT+GGG 0.560327 4.1:+13475998 MS.gene058818:CDS
GGTCGATACGCTCAACGTGT+TGG 0.571007 4.1:+13475847 MS.gene058818:CDS
ATCGACCTTTCTTCAAGTAA+CGG 0.579425 4.1:-13475831 None:intergenic
GGCTAGGTAAACAGGAGCAC+CGG 0.582435 4.1:-13475872 None:intergenic
GGAGCTGGAGGAAGGAAAGG+CGG 0.593373 4.1:+13475739 MS.gene058818:CDS
AACGGCCAATTCTTCCGACT+GGG 0.600319 4.1:-13475813 None:intergenic
GTAACAAGATCAATCAGAGC+CGG 0.606604 4.1:+13475781 MS.gene058818:CDS
GATATTCAAGAACTGCGGCT+AGG 0.617853 4.1:-13475888 None:intergenic
AAGAACACCACCATGAGCAA+TGG 0.624982 4.1:-13476040 None:intergenic
GCCGGACTTCAATTCCCAGT+CGG 0.639243 4.1:+13475799 MS.gene058818:CDS
CGGAGGACCAAGGAAGAAGT+CGG 0.640675 4.1:+13475759 MS.gene058818:CDS
GCGACGAAGAAAGGAGCTGG+AGG 0.642298 4.1:+13475727 MS.gene058818:CDS
GAAAATGGACGCAAGCACAA+AGG 0.663268 4.1:+13475705 None:intergenic
GGCATGTGTTGTTGGCTGTG+AGG 0.671767 4.1:+13475980 MS.gene058818:CDS
AGAAGAATCGGATTATACCA+AGG 0.672842 4.1:+13475959 MS.gene058818:CDS
AGCAAGATATTCAAGAACTG+CGG 0.685369 4.1:-13475893 None:intergenic
GGTGTTACCATTGCTCATGG+TGG 0.690637 4.1:+13476033 MS.gene058818:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAGAACAGGGTTAATATT+AGG - chr4.1:13476066-13476085 None:intergenic 25.0%
! TTTTCTTAGGCAAAAGAACA+GGG - chr4.1:13476077-13476096 None:intergenic 30.0%
AGAAGAATCGGATTATACCA+AGG + chr4.1:13475959-13475978 MS.gene058818:CDS 35.0%
AGCAAGATATTCAAGAACTG+CGG - chr4.1:13475896-13475915 None:intergenic 35.0%
ATCGACCTTTCTTCAAGTAA+CGG - chr4.1:13475834-13475853 None:intergenic 35.0%
CACGTGATAACAAGAAGAAT+CGG + chr4.1:13475947-13475966 MS.gene058818:CDS 35.0%
GAAAACTGAGAAAGCTGTTA+AGG + chr4.1:13476092-13476111 MS.gene058818:CDS 35.0%
! ACAGCTTTCTCAGTTTTCTT+AGG - chr4.1:13476090-13476109 None:intergenic 35.0%
! GTTTTCTTAGGCAAAAGAAC+AGG - chr4.1:13476078-13476097 None:intergenic 35.0%
!! TTAGCCTTAGAAGGAGATTT+AGG - chr4.1:13476120-13476139 None:intergenic 35.0%
ATTGGCCGTTACTTGAAGAA+AGG + chr4.1:13475826-13475845 MS.gene058818:CDS 40.0%
GGCTAAAAAGTCTCCTAAGA+AGG + chr4.1:13476134-13476153 MS.gene058818:CDS 40.0%
GTAACAAGATCAATCAGAGC+CGG + chr4.1:13475781-13475800 MS.gene058818:CDS 40.0%
TATACCAAGGCATGTGTTGT+TGG + chr4.1:13475972-13475991 MS.gene058818:CDS 40.0%
TGAGGAATGATGAAGAGCTT+GGG + chr4.1:13475998-13476017 MS.gene058818:CDS 40.0%
TGCTGCTGAAGTTCTTGAAT+TGG + chr4.1:13475912-13475931 MS.gene058818:CDS 40.0%
!!! GGAGACTTTTTAGCCTTAGA+AGG - chr4.1:13476129-13476148 None:intergenic 40.0%
AAGAACACCACCATGAGCAA+TGG - chr4.1:13476043-13476062 None:intergenic 45.0%
CAATTCCCAGTCGGAAGAAT+TGG + chr4.1:13475808-13475827 MS.gene058818:CDS 45.0%
GAGCTTGGGAAATTGCTTAC+TGG + chr4.1:13476012-13476031 MS.gene058818:CDS 45.0%
GATATTCAAGAACTGCGGCT+AGG - chr4.1:13475891-13475910 None:intergenic 45.0%
GCTGAAGTTCTTGAATTGGC+TGG + chr4.1:13475916-13475935 MS.gene058818:CDS 45.0%
GGAGCCTAAATCTCCTTCTA+AGG + chr4.1:13476113-13476132 MS.gene058818:CDS 45.0%
GTGAGGAATGATGAAGAGCT+TGG + chr4.1:13475997-13476016 MS.gene058818:CDS 45.0%
!! ACTGGTGTTACCATTGCTCA+TGG + chr4.1:13476030-13476049 MS.gene058818:CDS 45.0%
!! CTTGTTACCGACTTCTTCCT+TGG - chr4.1:13475769-13475788 None:intergenic 45.0%
AAAGGAGCTGGAGGAAGGAA+AGG + chr4.1:13475736-13475755 MS.gene058818:CDS 50.0%
AACGGCCAATTCTTCCGACT+GGG - chr4.1:13475816-13475835 None:intergenic 50.0%
ACAGCCAACAACACATGCCT+TGG - chr4.1:13475979-13475998 None:intergenic 50.0%
AGAACTGCGGCTAGGTAAAC+AGG - chr4.1:13475883-13475902 None:intergenic 50.0%
AGCACAAAGGCGACGAAGAA+AGG + chr4.1:13475718-13475737 MS.gene058818:CDS 50.0%
TAACGGCCAATTCTTCCGAC+TGG - chr4.1:13475817-13475836 None:intergenic 50.0%
TCCGACTGGGAATTGAAGTC+CGG - chr4.1:13475803-13475822 None:intergenic 50.0%
! GGTGTTACCATTGCTCATGG+TGG + chr4.1:13476033-13476052 MS.gene058818:CDS 50.0%
AAGGCGACGAAGAAAGGAGC+TGG + chr4.1:13475724-13475743 MS.gene058818:CDS 55.0%
CGAAGAAAGGAGCTGGAGGA+AGG + chr4.1:13475731-13475750 MS.gene058818:CDS 55.0%
CGGAGGACCAAGGAAGAAGT+CGG + chr4.1:13475759-13475778 MS.gene058818:CDS 55.0%
GCCGGACTTCAATTCCCAGT+CGG + chr4.1:13475799-13475818 MS.gene058818:CDS 55.0%
GGTCGATACGCTCAACGTGT+TGG + chr4.1:13475847-13475866 MS.gene058818:CDS 55.0%
! GGCATGTGTTGTTGGCTGTG+AGG + chr4.1:13475980-13475999 MS.gene058818:CDS 55.0%
! GGCTAGGTAAACAGGAGCAC+CGG - chr4.1:13475875-13475894 None:intergenic 55.0%
! TACGCTCAACGTGTTGGCAC+CGG + chr4.1:13475853-13475872 MS.gene058818:CDS 55.0%
GAAGGAAAGGCGGAGGACCA+AGG + chr4.1:13475749-13475768 MS.gene058818:CDS 60.0%
GCGACGAAGAAAGGAGCTGG+AGG + chr4.1:13475727-13475746 MS.gene058818:CDS 60.0%
GGAGCTGGAGGAAGGAAAGG+CGG + chr4.1:13475739-13475758 MS.gene058818:CDS 60.0%
GCTGGAGGAAGGAAAGGCGG+AGG + chr4.1:13475742-13475761 MS.gene058818:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13475709 13476161 13475709 ID=MS.gene058818
chr4.1 mRNA 13475709 13476161 13475709 ID=MS.gene058818.t1;Parent=MS.gene058818
chr4.1 exon 13475709 13476161 13475709 ID=MS.gene058818.t1.exon1;Parent=MS.gene058818.t1
chr4.1 CDS 13475709 13476161 13475709 ID=cds.MS.gene058818.t1;Parent=MS.gene058818.t1
Gene Sequence

>MS.gene058818

ATGGACGCAAGCACAAAGGCGACGAAGAAAGGAGCTGGAGGAAGGAAAGGCGGAGGACCAAGGAAGAAGTCGGTAACAAGATCAATCAGAGCCGGACTTCAATTCCCAGTCGGAAGAATTGGCCGTTACTTGAAGAAAGGTCGATACGCTCAACGTGTTGGCACCGGTGCTCCTGTTTACCTAGCCGCAGTTCTTGAATATCTTGCTGCTGAAGTTCTTGAATTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATCGGATTATACCAAGGCATGTGTTGTTGGCTGTGAGGAATGATGAAGAGCTTGGGAAATTGCTTACTGGTGTTACCATTGCTCATGGTGGTGTTCTTCCTAATATTAACCCTGTTCTTTTGCCTAAGAAAACTGAGAAAGCTGTTAAGGAGCCTAAATCTCCTTCTAAGGCTAAAAAGTCTCCTAAGAAGGCTTAG

Protein sequence

>MS.gene058818.t1

MDASTKATKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLTGVTIAHGGVLPNINPVLLPKKTEKAVKEPKSPSKAKKSPKKA