Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54339.t1 | AFK42063.1 | 97.6 | 84 | 2 | 0 | 1 | 84 | 63 | 146 | 1.90E-41 | 177.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54339.t1 | Q9FY93 | 77.5 | 80 | 18 | 0 | 1 | 80 | 62 | 141 | 2.5e-34 | 145.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54339.t1 | G7KVL9 | 97.6 | 84 | 2 | 0 | 1 | 84 | 63 | 146 | 1.4e-41 | 177.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052586 | MS.gene54339 | 0.805835 | 1.10E-49 | -1.69E-46 |
| MS.gene052685 | MS.gene54339 | 0.83201 | 1.21E-55 | -1.69E-46 |
| MS.gene053963 | MS.gene54339 | 0.856225 | 3.71E-62 | -1.69E-46 |
| MS.gene054222 | MS.gene54339 | 0.801667 | 8.08E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene54339 | 0.83518 | 1.95E-56 | -1.69E-46 |
| MS.gene060012 | MS.gene54339 | 0.807995 | 3.85E-50 | -1.69E-46 |
| MS.gene060412 | MS.gene54339 | 0.810703 | 1.01E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGGTTCCCATTTGGGTATT+TGG | 0.189837 | 7.2:-93332252 | None:intergenic |
| CTGTTAGATCGGTTCCCATT+TGG | 0.268180 | 7.2:-93332260 | None:intergenic |
| TGTTAGATCGGTTCCCATTT+GGG | 0.328133 | 7.2:-93332259 | None:intergenic |
| GAGCTACAAACTCTGGTTAC+TGG | 0.339051 | 7.2:+93332282 | MS.gene54339:CDS |
| AAAGAAGCCAAATACCCAAA+TGG | 0.451382 | 7.2:+93332245 | MS.gene54339:CDS |
| TCTGGTTACTGGAAAGCAAC+TGG | 0.454286 | 7.2:+93332293 | MS.gene54339:CDS |
| TCAAAGAGTCATGAAGTTGT+TGG | 0.455914 | 7.2:+93332338 | MS.gene54339:CDS |
| AAGAAGCCAAATACCCAAAT+GGG | 0.481949 | 7.2:+93332246 | MS.gene54339:CDS |
| TTCCTCTCACTCCAACCCCT+TGG | 0.484311 | 7.2:+93332445 | MS.gene54339:CDS |
| GGTTGGAGTGAGAGGAAGTG+AGG | 0.514880 | 7.2:-93332439 | None:intergenic |
| AGTTTGTAGCTCTGTTAGAT+CGG | 0.532621 | 7.2:-93332271 | None:intergenic |
| GACCAAGGGGTTGGAGTGAG+AGG | 0.548262 | 7.2:-93332447 | None:intergenic |
| CTCATGGTTCAAGAACTGAT+TGG | 0.548704 | 7.2:+93332399 | MS.gene54339:CDS |
| AGAAAGACTGACCAAGGGGT+TGG | 0.548887 | 7.2:-93332456 | None:intergenic |
| TCTAACAGAGCTACAAACTC+TGG | 0.560803 | 7.2:+93332275 | MS.gene54339:CDS |
| TACAGAGGCAAACCACCTCA+TGG | 0.581454 | 7.2:+93332383 | MS.gene54339:CDS |
| TCAGTTCTTGAACCATGAGG+TGG | 0.615010 | 7.2:-93332395 | None:intergenic |
| TAGGTGATATGGAACAAGAG+AGG | 0.664397 | 7.2:+93332207 | None:intergenic |
| CAATCAGTTCTTGAACCATG+AGG | 0.707588 | 7.2:-93332398 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAACTCTTGTTTTCTACAG+AGG | + | chr7.2:93332368-93332387 | MS.gene54339:CDS | 30.0% |
| AAAGAAGCCAAATACCCAAA+TGG | + | chr7.2:93332245-93332264 | MS.gene54339:CDS | 35.0% | |
| AAGAAGCCAAATACCCAAAT+GGG | + | chr7.2:93332246-93332265 | MS.gene54339:CDS | 35.0% | |
| TCAAAGAGTCATGAAGTTGT+TGG | + | chr7.2:93332338-93332357 | MS.gene54339:CDS | 35.0% | |
| ! | AGTTTGTAGCTCTGTTAGAT+CGG | - | chr7.2:93332274-93332293 | None:intergenic | 35.0% |
| CAATCAGTTCTTGAACCATG+AGG | - | chr7.2:93332401-93332420 | None:intergenic | 40.0% | |
| CTCATGGTTCAAGAACTGAT+TGG | + | chr7.2:93332399-93332418 | MS.gene54339:CDS | 40.0% | |
| TCTAACAGAGCTACAAACTC+TGG | + | chr7.2:93332275-93332294 | MS.gene54339:CDS | 40.0% | |
| ! | TGTTAGATCGGTTCCCATTT+GGG | - | chr7.2:93332262-93332281 | None:intergenic | 40.0% |
| GAGCTACAAACTCTGGTTAC+TGG | + | chr7.2:93332282-93332301 | MS.gene54339:CDS | 45.0% | |
| TCAGTTCTTGAACCATGAGG+TGG | - | chr7.2:93332398-93332417 | None:intergenic | 45.0% | |
| TCGGTTCCCATTTGGGTATT+TGG | - | chr7.2:93332255-93332274 | None:intergenic | 45.0% | |
| TCTGGTTACTGGAAAGCAAC+TGG | + | chr7.2:93332293-93332312 | MS.gene54339:CDS | 45.0% | |
| ! | CTGTTAGATCGGTTCCCATT+TGG | - | chr7.2:93332263-93332282 | None:intergenic | 45.0% |
| TACAGAGGCAAACCACCTCA+TGG | + | chr7.2:93332383-93332402 | MS.gene54339:CDS | 50.0% | |
| GGTTGGAGTGAGAGGAAGTG+AGG | - | chr7.2:93332442-93332461 | None:intergenic | 55.0% | |
| TTCCTCTCACTCCAACCCCT+TGG | + | chr7.2:93332445-93332464 | MS.gene54339:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 93332210 | 93332466 | 93332210 | ID=MS.gene54339 |
| chr7.2 | mRNA | 93332210 | 93332466 | 93332210 | ID=MS.gene54339.t1;Parent=MS.gene54339 |
| chr7.2 | exon | 93332210 | 93332466 | 93332210 | ID=MS.gene54339.t1.exon1;Parent=MS.gene54339.t1 |
| chr7.2 | CDS | 93332210 | 93332466 | 93332210 | ID=cds.MS.gene54339.t1;Parent=MS.gene54339.t1 |
| Gene Sequence |
| Protein sequence |
