AlfalfaGEDB | MS.gene052685

M. sativa cultivar XinJiangDaYe / MS.gene052685

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052685.t1	RHN39340.1	85.6	181	26	0	1	181	202	382	2.00E-19	105.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052685.t1	G7LI81	85.6	181	26	0	1	181	1054	1234	1.4e-19	105.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene052685	0.800149	1.65E-48	-1.69E-46
MS.gene04920	MS.gene052685	0.810131	1.34E-50	-1.69E-46
MS.gene049254	MS.gene052685	0.802186	6.32E-49	-1.69E-46
MS.gene049499	MS.gene052685	0.801665	8.09E-49	-1.69E-46
MS.gene049567	MS.gene052685	0.809135	2.20E-50	-1.69E-46
MS.gene050026	MS.gene052685	0.800005	1.77E-48	-1.69E-46
MS.gene050355	MS.gene052685	0.810422	1.16E-50	-1.69E-46
MS.gene052463	MS.gene052685	0.806744	7.09E-50	-1.69E-46
MS.gene052519	MS.gene052685	0.810247	1.27E-50	-1.69E-46
MS.gene052685	MS.gene053006	0.81878	1.64E-52	-1.69E-46
MS.gene052685	MS.gene053298	0.802611	5.17E-49	-1.69E-46
MS.gene052685	MS.gene05365	0.831172	1.94E-55	-1.69E-46
MS.gene052685	MS.gene053963	0.849468	3.17E-60	-1.69E-46
MS.gene052685	MS.gene054222	0.823795	1.14E-53	-1.69E-46
MS.gene052685	MS.gene054892	0.807004	6.25E-50	-1.69E-46
MS.gene052685	MS.gene054893	0.805942	1.05E-49	-1.69E-46
MS.gene052685	MS.gene055801	0.820773	5.74E-53	-1.69E-46
MS.gene052685	MS.gene056174	0.807496	4.91E-50	-1.69E-46
MS.gene052685	MS.gene056779	0.800172	1.63E-48	-1.69E-46
MS.gene052685	MS.gene05686	0.800494	1.40E-48	-1.69E-46
MS.gene052685	MS.gene057102	0.801006	1.10E-48	-1.69E-46
MS.gene052685	MS.gene057208	0.810788	9.68E-51	-1.69E-46
MS.gene052685	MS.gene058037	0.825497	4.54E-54	-1.69E-46
MS.gene052685	MS.gene058159	0.819388	1.19E-52	-1.69E-46
MS.gene052685	MS.gene05952	0.830681	2.56E-55	-1.69E-46
MS.gene052685	MS.gene060521	0.808267	3.37E-50	-1.69E-46
MS.gene052685	MS.gene060879	0.81242	4.28E-51	-1.69E-46
MS.gene052685	MS.gene063247	0.811336	7.37E-51	-1.69E-46
MS.gene052685	MS.gene06372	0.809278	2.05E-50	-1.69E-46
MS.gene052685	MS.gene064969	0.815608	8.47E-52	-1.69E-46
MS.gene052685	MS.gene065460	0.81272	3.68E-51	-1.69E-46
MS.gene052685	MS.gene06643	0.808288	3.33E-50	-1.69E-46
MS.gene052685	MS.gene066513	0.829672	4.52E-55	-1.69E-46
MS.gene052685	MS.gene068350	0.839444	1.58E-57	-1.69E-46
MS.gene052685	MS.gene06857	0.805685	1.18E-49	-1.69E-46
MS.gene052685	MS.gene069024	0.832386	9.74E-56	-1.69E-46
MS.gene052685	MS.gene070210	0.802009	6.87E-49	-1.69E-46
MS.gene052685	MS.gene070275	0.802265	6.09E-49	-1.69E-46
MS.gene052685	MS.gene070301	0.818656	1.75E-52	-1.69E-46
MS.gene052685	MS.gene071034	0.823657	1.23E-53	-1.69E-46
MS.gene052685	MS.gene071342	0.807125	5.89E-50	-1.69E-46
MS.gene052685	MS.gene07141	0.805752	1.15E-49	-1.69E-46
MS.gene052685	MS.gene071569	0.814205	1.73E-51	-1.69E-46
MS.gene052685	MS.gene071605	-0.802825	4.67E-49	-1.69E-46
MS.gene052685	MS.gene072029	0.803929	2.76E-49	-1.69E-46
MS.gene052685	MS.gene072213	0.812323	4.49E-51	-1.69E-46
MS.gene052685	MS.gene072280	0.809145	2.19E-50	-1.69E-46
MS.gene052685	MS.gene072299	0.814047	1.88E-51	-1.69E-46
MS.gene052685	MS.gene072955	0.817803	2.73E-52	-1.69E-46
MS.gene052685	MS.gene073028	0.822094	2.85E-53	-1.69E-46
MS.gene052685	MS.gene073029	0.810634	1.05E-50	-1.69E-46
MS.gene052685	MS.gene073139	0.807706	4.43E-50	-1.69E-46
MS.gene052685	MS.gene073428	0.811889	5.59E-51	-1.69E-46
MS.gene052685	MS.gene07394	0.808354	3.23E-50	-1.69E-46
MS.gene052685	MS.gene074100	0.811054	8.48E-51	-1.69E-46
MS.gene052685	MS.gene074381	0.801172	1.02E-48	-1.69E-46
MS.gene052685	MS.gene074603	0.803557	3.29E-49	-1.69E-46
MS.gene052685	MS.gene07815	0.935595	6.62E-97	-1.69E-46
MS.gene052685	MS.gene08862	0.828286	9.78E-55	-1.69E-46
MS.gene052685	MS.gene09233	0.829998	3.76E-55	-1.69E-46
MS.gene052685	MS.gene20608	0.814539	1.46E-51	-1.69E-46
MS.gene052685	MS.gene20610	0.832534	8.95E-56	-1.69E-46
MS.gene052685	MS.gene22557	0.807334	5.32E-50	-1.69E-46
MS.gene052685	MS.gene22992	-0.806144	9.49E-50	-1.69E-46
MS.gene052685	MS.gene25151	0.803817	2.91E-49	-1.69E-46
MS.gene052685	MS.gene25755	0.801602	8.33E-49	-1.69E-46
MS.gene052685	MS.gene25860	0.800744	1.25E-48	-1.69E-46
MS.gene052685	MS.gene25978	0.813503	2.48E-51	-1.69E-46
MS.gene052685	MS.gene25991	0.836697	8.04E-57	-1.69E-46
MS.gene052685	MS.gene26958	0.805521	1.28E-49	-1.69E-46
MS.gene052685	MS.gene27318	0.807511	4.88E-50	-1.69E-46
MS.gene052685	MS.gene27381	0.802347	5.86E-49	-1.69E-46
MS.gene052685	MS.gene27516	0.841217	5.43E-58	-1.69E-46
MS.gene052685	MS.gene28918	0.809258	2.07E-50	-1.69E-46
MS.gene052685	MS.gene29036	0.813716	2.22E-51	-1.69E-46
MS.gene052685	MS.gene29096	0.811841	5.72E-51	-1.69E-46
MS.gene052685	MS.gene29428	0.822024	2.96E-53	-1.69E-46
MS.gene052685	MS.gene30085	0.802197	6.29E-49	-1.69E-46
MS.gene052685	MS.gene30368	0.801087	1.06E-48	-1.69E-46
MS.gene052685	MS.gene30663	0.81008	1.38E-50	-1.69E-46
MS.gene052685	MS.gene30970	0.819833	9.44E-53	-1.69E-46
MS.gene052685	MS.gene31142	0.820791	5.69E-53	-1.69E-46
MS.gene052685	MS.gene31551	0.801426	9.06E-49	-1.69E-46
MS.gene052685	MS.gene31855	0.851932	6.43E-61	-1.69E-46
MS.gene052685	MS.gene32176	0.827302	1.69E-54	-1.69E-46
MS.gene052685	MS.gene32409	0.802487	5.48E-49	-1.69E-46
MS.gene052685	MS.gene32579	0.809398	1.93E-50	-1.69E-46
MS.gene052685	MS.gene32801	0.827077	1.91E-54	-1.69E-46
MS.gene052685	MS.gene33131	0.809537	1.80E-50	-1.69E-46
MS.gene052685	MS.gene33650	0.818539	1.86E-52	-1.69E-46
MS.gene052685	MS.gene33651	0.868568	5.93E-66	-1.69E-46
MS.gene052685	MS.gene33751	0.82592	3.60E-54	-1.69E-46
MS.gene052685	MS.gene34444	0.820408	6.97E-53	-1.69E-46
MS.gene052685	MS.gene35324	0.810514	1.11E-50	-1.69E-46
MS.gene052685	MS.gene36100	0.802255	6.12E-49	-1.69E-46
MS.gene052685	MS.gene36151	0.807629	4.60E-50	-1.69E-46
MS.gene052685	MS.gene36229	0.820955	5.22E-53	-1.69E-46
MS.gene052685	MS.gene36231	0.818091	2.35E-52	-1.69E-46
MS.gene052685	MS.gene36538	-0.821119	4.78E-53	-1.69E-46
MS.gene052685	MS.gene36740	0.804924	1.71E-49	-1.69E-46
MS.gene052685	MS.gene37412	0.821022	5.04E-53	-1.69E-46
MS.gene052685	MS.gene37612	0.854554	1.14E-61	-1.69E-46
MS.gene052685	MS.gene39037	0.811969	5.37E-51	-1.69E-46
MS.gene052685	MS.gene40123	0.809778	1.60E-50	-1.69E-46
MS.gene052685	MS.gene40709	0.825338	4.95E-54	-1.69E-46
MS.gene052685	MS.gene40711	0.823772	1.16E-53	-1.69E-46
MS.gene052685	MS.gene40908	0.803634	3.17E-49	-1.69E-46
MS.gene052685	MS.gene41464	0.800904	1.16E-48	-1.69E-46
MS.gene052685	MS.gene42142	0.80278	4.77E-49	-1.69E-46
MS.gene052685	MS.gene43372	0.807639	4.58E-50	-1.69E-46
MS.gene052685	MS.gene43381	0.802162	6.40E-49	-1.69E-46
MS.gene052685	MS.gene44058	0.824708	6.97E-54	-1.69E-46
MS.gene052685	MS.gene44179	0.802124	6.51E-49	-1.69E-46
MS.gene052685	MS.gene44830	0.800891	1.17E-48	-1.69E-46
MS.gene052685	MS.gene45507	0.803238	3.83E-49	-1.69E-46
MS.gene052685	MS.gene47195	0.807914	4.00E-50	-1.69E-46
MS.gene052685	MS.gene47759	0.810871	9.29E-51	-1.69E-46
MS.gene052685	MS.gene48106	0.813331	2.70E-51	-1.69E-46
MS.gene052685	MS.gene49386	0.804633	1.97E-49	-1.69E-46
MS.gene052685	MS.gene50546	0.805164	1.52E-49	-1.69E-46
MS.gene052685	MS.gene51957	0.824254	8.92E-54	-1.69E-46
MS.gene052685	MS.gene52041	0.810815	9.55E-51	-1.69E-46
MS.gene052685	MS.gene52130	0.812687	3.74E-51	-1.69E-46
MS.gene052685	MS.gene53460	0.801229	9.94E-49	-1.69E-46
MS.gene052685	MS.gene53535	0.803045	4.20E-49	-1.69E-46
MS.gene052685	MS.gene53537	0.822198	2.69E-53	-1.69E-46
MS.gene052685	MS.gene54090	0.828039	1.12E-54	-1.69E-46
MS.gene052685	MS.gene54339	0.83201	1.21E-55	-1.69E-46
MS.gene052685	MS.gene54703	0.806771	7.00E-50	-1.69E-46
MS.gene052685	MS.gene54704	0.821051	4.96E-53	-1.69E-46
MS.gene052685	MS.gene55540	0.803278	3.76E-49	-1.69E-46
MS.gene052685	MS.gene55545	0.804109	2.53E-49	-1.69E-46
MS.gene052685	MS.gene57128	0.833155	6.27E-56	-1.69E-46
MS.gene052685	MS.gene57498	0.813193	2.90E-51	-1.69E-46
MS.gene052685	MS.gene58081	0.81041	1.17E-50	-1.69E-46
MS.gene052685	MS.gene58547	0.815572	8.63E-52	-1.69E-46
MS.gene052685	MS.gene58638	0.816837	4.50E-52	-1.69E-46
MS.gene052685	MS.gene58653	0.805863	1.09E-49	-1.69E-46
MS.gene052685	MS.gene58802	0.800802	1.21E-48	-1.69E-46
MS.gene052685	MS.gene59540	0.850938	1.23E-60	-1.69E-46
MS.gene052685	MS.gene59616	0.81194	5.44E-51	-1.69E-46
MS.gene052685	MS.gene59697	0.830049	3.65E-55	-1.69E-46
MS.gene052685	MS.gene60384	0.832616	8.54E-56	-1.69E-46
MS.gene052685	MS.gene61162	0.807842	4.15E-50	-1.69E-46
MS.gene052685	MS.gene62676	0.83958	1.46E-57	-1.69E-46
MS.gene052685	MS.gene62677	0.858176	9.86E-63	-1.69E-46
MS.gene052685	MS.gene62887	-0.81555	8.73E-52	-1.69E-46
MS.gene052685	MS.gene63430	0.81888	1.56E-52	-1.69E-46
MS.gene052685	MS.gene64284	0.82712	1.86E-54	-1.69E-46
MS.gene052685	MS.gene65673	0.815401	9.42E-52	-1.69E-46
MS.gene052685	MS.gene66077	0.800269	1.56E-48	-1.69E-46
MS.gene052685	MS.gene66545	0.803941	2.74E-49	-1.69E-46
MS.gene052685	MS.gene68002	0.817333	3.48E-52	-1.69E-46
MS.gene052685	MS.gene68198	0.833427	5.37E-56	-1.69E-46
MS.gene052685	MS.gene68824	0.819621	1.05E-52	-1.69E-46
MS.gene052685	MS.gene68826	0.803308	3.71E-49	-1.69E-46
MS.gene052685	MS.gene68999	0.812482	4.15E-51	-1.69E-46
MS.gene052685	MS.gene69780	0.817368	3.42E-52	-1.69E-46
MS.gene052685	MS.gene69821	0.808365	3.21E-50	-1.69E-46
MS.gene052685	MS.gene70656	0.836248	1.05E-56	-1.69E-46
MS.gene052685	MS.gene71735	0.800755	1.24E-48	-1.69E-46
MS.gene052685	MS.gene71803	0.805397	1.36E-49	-1.69E-46
MS.gene052685	MS.gene71806	0.815769	7.80E-52	-1.69E-46
MS.gene052685	MS.gene71828	0.821933	3.10E-53	-1.69E-46
MS.gene052685	MS.gene71945	0.823114	1.65E-53	-1.69E-46
MS.gene052685	MS.gene72026	0.836116	1.13E-56	-1.69E-46
MS.gene052685	MS.gene72748	0.815469	9.10E-52	-1.69E-46
MS.gene052685	MS.gene73467	0.811682	6.19E-51	-1.69E-46
MS.gene052685	MS.gene73678	0.805068	1.60E-49	-1.69E-46
MS.gene052685	MS.gene73820	0.800131	1.66E-48	-1.69E-46
MS.gene052685	MS.gene73831	0.805435	1.34E-49	-1.69E-46
MS.gene052685	MS.gene74530	0.817237	3.66E-52	-1.69E-46
MS.gene052685	MS.gene74537	0.811311	7.46E-51	-1.69E-46
MS.gene052685	MS.gene74604	0.81113	8.17E-51	-1.69E-46
MS.gene052685	MS.gene74956	0.809604	1.74E-50	-1.69E-46
MS.gene052685	MS.gene75052	0.822395	2.42E-53	-1.69E-46
MS.gene052685	MS.gene75139	0.800279	1.55E-48	-1.69E-46
MS.gene052685	MS.gene75206	0.811999	5.28E-51	-1.69E-46
MS.gene052685	MS.gene76148	0.816527	5.28E-52	-1.69E-46
MS.gene052685	MS.gene76244	0.842811	2.06E-58	-1.69E-46
MS.gene052685	MS.gene77361	0.810353	1.20E-50	-1.69E-46
MS.gene052685	MS.gene77412	0.830238	3.29E-55	-1.69E-46
MS.gene052685	MS.gene78642	0.844208	8.71E-59	-1.69E-46
MS.gene052685	MS.gene78773	0.802147	6.44E-49	-1.69E-46
MS.gene052685	MS.gene79568	0.842839	2.02E-58	-1.69E-46
MS.gene052685	MS.gene80382	0.804009	2.65E-49	-1.69E-46
MS.gene052685	MS.gene80821	0.804419	2.18E-49	-1.69E-46
MS.gene052685	MS.gene81272	0.808284	3.34E-50	-1.69E-46
MS.gene052685	MS.gene82568	0.809286	2.04E-50	-1.69E-46
MS.gene052685	MS.gene82800	0.810769	9.77E-51	-1.69E-46
MS.gene052685	MS.gene83312	0.837388	5.35E-57	-1.69E-46
MS.gene052685	MS.gene83473	0.816295	5.95E-52	-1.69E-46
MS.gene052685	MS.gene83687	0.810737	9.94E-51	-1.69E-46
MS.gene052685	MS.gene83853	0.804585	2.01E-49	-1.69E-46
MS.gene052685	MS.gene84162	0.821973	3.04E-53	-1.69E-46
MS.gene052685	MS.gene84732	0.815979	7.00E-52	-1.69E-46
MS.gene052685	MS.gene84865	0.805945	1.04E-49	-1.69E-46
MS.gene052685	MS.gene85257	0.819732	9.95E-53	-1.69E-46
MS.gene052685	MS.gene85936	0.826433	2.72E-54	-1.69E-46
MS.gene052685	MS.gene85978	0.833208	6.09E-56	-1.69E-46
MS.gene052685	MS.gene85979	0.82074	5.85E-53	-1.69E-46
MS.gene052685	MS.gene86455	0.802338	5.88E-49	-1.69E-46
MS.gene052685	MS.gene87344	0.809854	1.54E-50	-1.69E-46
MS.gene052685	MS.gene87673	0.843	1.83E-58	-1.69E-46
MS.gene052685	MS.gene88351	0.84765	1.01E-59	-1.69E-46
MS.gene052685	MS.gene89049	0.806496	8.00E-50	-1.69E-46
MS.gene052685	MS.gene89255	0.80413	2.50E-49	-1.69E-46
MS.gene052685	MS.gene89350	0.802543	5.34E-49	-1.69E-46
MS.gene052685	MS.gene90413	0.831633	1.49E-55	-1.69E-46
MS.gene052685	MS.gene90651	0.831645	1.48E-55	-1.69E-46
MS.gene052685	MS.gene91204	0.84104	6.05E-58	-1.69E-46
MS.gene052685	MS.gene91996	0.82774	1.32E-54	-1.69E-46
MS.gene052685	MS.gene92073	0.800754	1.24E-48	-1.69E-46
MS.gene052685	MS.gene92320	0.841898	3.59E-58	-1.69E-46
MS.gene052685	MS.gene92364	0.822511	2.28E-53	-1.69E-46
MS.gene052685	MS.gene92646	0.813634	2.32E-51	-1.69E-46
MS.gene052685	MS.gene92775	0.85081	1.33E-60	-1.69E-46
MS.gene052685	MS.gene93155	0.812302	4.54E-51	-1.69E-46
MS.gene052685	MS.gene93764	0.804365	2.24E-49	-1.69E-46
MS.gene052685	MS.gene94111	0.803881	2.82E-49	-1.69E-46
MS.gene052685	MS.gene94674	0.826188	3.11E-54	-1.69E-46
MS.gene052685	MS.gene94906	0.814968	1.18E-51	-1.69E-46
MS.gene052685	MS.gene94908	0.816259	6.06E-52	-1.69E-46
MS.gene052685	MS.gene95305	0.811401	7.13E-51	-1.69E-46
MS.gene052685	MS.gene95655	0.849539	3.03E-60	-1.69E-46
MS.gene052685	MS.gene95936	0.803665	3.13E-49	-1.69E-46
MS.gene052685	MS.gene96324	0.822241	2.63E-53	-1.69E-46
MS.gene052685	MS.gene96973	0.824234	9.01E-54	-1.69E-46
MS.gene052685	MS.gene97447	0.834404	3.06E-56	-1.69E-46
MS.gene052685	MS.gene97534	0.818889	1.55E-52	-1.69E-46
MS.gene052685	MS.gene98179	0.816245	6.11E-52	-1.69E-46
MS.gene052685	MS.gene99483	0.829347	5.42E-55	-1.69E-46
MS.gene052685	MS.gene99894	0.823122	1.64E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052685.t1	MTR_8g018270	86.740	181	24	1	181	1054	1234	2.84e-87	278
MS.gene052685.t1	MTR_8g018280	75.000	180	45	2	181	1294	1473	8.62e-75	244
MS.gene052685.t1	MTR_5g086690	74.419	172	44	2	173	1308	1479	1.90e-71	234
MS.gene052685.t1	MTR_8g018160	69.231	39	12	25	63	1	39	7.31e-12	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 22 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGCCATCAGCTCTTTGCTTT+CGG	0.279935	8.1:-79902584	None:intergenic
CAATAGAGGATGTCATATTA+TGG	0.331248	8.1:-79902246	None:intergenic
TCAACTTCTGCTGCTTTGAC+AGG	0.334818	8.1:-79902642	None:intergenic
GCGGCAATTACATATGTCTT+TGG	0.336095	8.1:+79902168	None:intergenic
ATAGAATTTGCAGACAAGTA+TGG	0.363847	8.1:+79902688	MS.gene052685:CDS
TGGACAAGCTTCGCAACATT+AGG	0.379490	8.1:-79902226	None:intergenic
AGCAGAAGTTGAAAGACAAA+TGG	0.425002	8.1:+79902653	MS.gene052685:CDS
GCAAAAGAAAATTCTTGAGT+TGG	0.438888	8.1:+79902480	MS.gene052685:CDS
TGAAAGTGAAAAGAAGAAAG+TGG	0.451582	8.1:+79902537	MS.gene052685:CDS
TAAAGAAAAGAGTCAGTTTG+AGG	0.469383	8.1:+79902393	MS.gene052685:CDS
TTCCATGCTCTCCTTCAACT+TGG	0.474707	8.1:-79902509	None:intergenic
GCAGAAGTTGAAAGACAAAT+GGG	0.480276	8.1:+79902654	MS.gene052685:CDS
ATTGAAAAGGTTCTCAATAG+AGG	0.496397	8.1:-79902260	None:intergenic
CAACTTCTGCTGCTTTGACA+GGG	0.502838	8.1:-79902641	None:intergenic
CCATGACGAAGTCATTGAAA+AGG	0.508796	8.1:-79902273	None:intergenic
AGCTAAGCATGTCGAGGAAG+AGG	0.530407	8.1:+79902420	MS.gene052685:CDS
AACCAAGTTGAAGGAGAGCA+TGG	0.531090	8.1:+79902507	MS.gene052685:CDS
AACCGAAAGCAAAGAGCTGA+TGG	0.557939	8.1:+79902582	MS.gene052685:CDS
AAAGAAGAAAGTGGAAGATG+AGG	0.561565	8.1:+79902546	MS.gene052685:CDS
GACAGATGCTAAGAAACAGA+AGG	0.626229	8.1:+79902360	MS.gene052685:CDS
AAGTTGAAAGACAAATGGGA+AGG	0.630448	8.1:+79902658	MS.gene052685:CDS
GGATGAAGCTAAGCATGTCG+AGG	0.685617	8.1:+79902414	MS.gene052685:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATAGAATTTGCAGACAAGTA+TGG	+	chr8.1:79902688-79902707	MS.gene052685:CDS	30.0%
	ATTGAAAAGGTTCTCAATAG+AGG	-	chr8.1:79902263-79902282	None:intergenic	30.0%
	CAATAGAGGATGTCATATTA+TGG	-	chr8.1:79902249-79902268	None:intergenic	30.0%
	GCAAAAGAAAATTCTTGAGT+TGG	+	chr8.1:79902480-79902499	MS.gene052685:CDS	30.0%
	TAAAGAAAAGAGTCAGTTTG+AGG	+	chr8.1:79902393-79902412	MS.gene052685:CDS	30.0%
	TGAAAGTGAAAAGAAGAAAG+TGG	+	chr8.1:79902537-79902556	MS.gene052685:CDS	30.0%
	AAAGAAGAAAGTGGAAGATG+AGG	+	chr8.1:79902546-79902565	MS.gene052685:CDS	35.0%
	AAGTTGAAAGACAAATGGGA+AGG	+	chr8.1:79902658-79902677	MS.gene052685:CDS	35.0%
	AGCAGAAGTTGAAAGACAAA+TGG	+	chr8.1:79902653-79902672	MS.gene052685:CDS	35.0%
	GCAGAAGTTGAAAGACAAAT+GGG	+	chr8.1:79902654-79902673	MS.gene052685:CDS	35.0%
!	GTTGGAAAAAACCAAGTTGA+AGG	+	chr8.1:79902498-79902517	MS.gene052685:CDS	35.0%
	CATGGCTAAAAATGTGGTTG+AGG	+	chr8.1:79902291-79902310	MS.gene052685:CDS	40.0%
	CCATGACGAAGTCATTGAAA+AGG	-	chr8.1:79902276-79902295	None:intergenic	40.0%
	CGAGGAAGAGGTTGAAAAAA+CGG	+	chr8.1:79902432-79902451	MS.gene052685:CDS	40.0%
	CTTCGTCATGGCTAAAAATG+TGG	+	chr8.1:79902285-79902304	MS.gene052685:CDS	40.0%
	GACAGATGCTAAGAAACAGA+AGG	+	chr8.1:79902360-79902379	MS.gene052685:CDS	40.0%
!	CCTTTTCAATGACTTCGTCA+TGG	+	chr8.1:79902273-79902292	MS.gene052685:CDS	40.0%
	AACCGAAAGCAAAGAGCTGA+TGG	+	chr8.1:79902582-79902601	MS.gene052685:CDS	45.0%
	TGGACAAGCTTCGCAACATT+AGG	-	chr8.1:79902229-79902248	None:intergenic	45.0%
	TTCCATGCTCTCCTTCAACT+TGG	-	chr8.1:79902512-79902531	None:intergenic	45.0%
!	AACCAAGTTGAAGGAGAGCA+TGG	+	chr8.1:79902507-79902526	MS.gene052685:CDS	45.0%
!!	CAACTTCTGCTGCTTTGACA+GGG	-	chr8.1:79902644-79902663	None:intergenic	45.0%
!!	TCAACTTCTGCTGCTTTGAC+AGG	-	chr8.1:79902645-79902664	None:intergenic	45.0%
	AGCTAAGCATGTCGAGGAAG+AGG	+	chr8.1:79902420-79902439	MS.gene052685:CDS	50.0%
	CGCCATCAGCTCTTTGCTTT+CGG	-	chr8.1:79902587-79902606	None:intergenic	50.0%
	GGATGAAGCTAAGCATGTCG+AGG	+	chr8.1:79902414-79902433	MS.gene052685:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	79902181	79902726	79902181	ID=MS.gene052685
chr8.1	mRNA	79902181	79902726	79902181	ID=MS.gene052685.t1;Parent=MS.gene052685
chr8.1	exon	79902181	79902726	79902181	ID=MS.gene052685.t1.exon1;Parent=MS.gene052685.t1
chr8.1	CDS	79902181	79902726	79902181	ID=cds.MS.gene052685.t1;Parent=MS.gene052685.t1

Gene Sequence

>MS.gene052685

ATGTCTTTGGAATGCATAAAACAAGAATCTCATGAAAAAGTTTCTCCTAATGTTGCGAAGCTTGTCCATAATATGACATCCTCTATTGAGAACCTTTTCAATGACTTCGTCATGGCTAAAAATGTGGTTGAGGATCACAATAATGCTTTACAACAAAAAGAAAAGCTTATACAGCTAGTGACAGATGCTAAGAAACAGAAGGAATCAAAACATAAAGAAAAGAGTCAGTTTGAGGATGAAGCTAAGCATGTCGAGGAAGAGGTTGAAAAAACGGATGAAAAAATTAGAATACTTGTCGAGCAAAAGAAAATTCTTGAGTTGGAAAAAACCAAGTTGAAGGAGAGCATGGAAAGATGTGAAAGTGAAAAGAAGAAAGTGGAAGATGAGGCTAAGAATATGATAACCGAAAGCAAAGAGCTGATGGCGAGTATTAAGAAGTCTAAATCTTCTTATACTGCTGCCCTGTCAAAGCAGCAGAAGTTGAAAGACAAATGGGAAGGTTTCAGAATAGAATTTGCAGACAAGTATGGAAGTAGCAGTAGCTGA

Protein sequence

>MS.gene052685.t1

MSLECIKQESHEKVSPNVAKLVHNMTSSIENLFNDFVMAKNVVEDHNNALQQKEKLIQLVTDAKKQKESKHKEKSQFEDEAKHVEEEVEKTDEKIRILVEQKKILELEKTKLKESMERCESEKKKVEDEAKNMITESKELMASIKKSKSSYTAALSKQQKLKDKWEGFRIEFADKYGSSSS