Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54344.t1 | XP_013447780.1 | 94 | 218 | 2 | 1 | 1 | 207 | 17 | 234 | 1.80E-101 | 378.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54344.t1 | P93332 | 92.7 | 218 | 5 | 1 | 1 | 207 | 17 | 234 | 3.1e-102 | 372.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54344.t1 | A0A072TX72 | 94.0 | 218 | 2 | 1 | 1 | 207 | 17 | 234 | 1.3e-101 | 378.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05240 | MS.gene54344 | 0.803474 | 3.43E-49 | -1.69E-46 |
| MS.gene054277 | MS.gene54344 | 0.807484 | 4.94E-50 | -1.69E-46 |
| MS.gene054742 | MS.gene54344 | 0.846123 | 2.65E-59 | -1.69E-46 |
| MS.gene056590 | MS.gene54344 | 0.802571 | 5.27E-49 | -1.69E-46 |
| MS.gene056591 | MS.gene54344 | 0.802597 | 5.20E-49 | -1.69E-46 |
| MS.gene056920 | MS.gene54344 | 0.840883 | 6.65E-58 | -1.69E-46 |
| MS.gene060087 | MS.gene54344 | 0.807853 | 4.13E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 51 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTCGTTCTTGGTGTTCT+TGG | 0.169280 | 7.3:-82123412 | MS.gene54344:intron |
| CACACATCCAAATGAGTTTA+TGG | 0.188728 | 7.3:+82123159 | None:intergenic |
| CCACTTCGTGTCCAAGTTCT+TGG | 0.244317 | 7.3:-82122882 | MS.gene54344:CDS |
| CTCATTACCATAAACTCATT+TGG | 0.319248 | 7.3:-82123166 | MS.gene54344:CDS |
| AGGTAACGTCATTTCGTTCT+TGG | 0.323739 | 7.3:-82123421 | MS.gene54344:CDS |
| CTTAACTTTCAAGTTACTTT+CGG | 0.328483 | 7.3:-82122964 | MS.gene54344:CDS |
| AATGTGTTGGGTTTCGTATT+GGG | 0.328786 | 7.3:-82122328 | MS.gene54344:CDS |
| CTTTGTTCAGCTCCATGCTT+TGG | 0.359151 | 7.3:-82123225 | MS.gene54344:CDS |
| AATACGAAACCCAACACATT+TGG | 0.368529 | 7.3:+82122331 | None:intergenic |
| AAATGTGTTGGGTTTCGTAT+TGG | 0.380441 | 7.3:-82122329 | MS.gene54344:CDS |
| CACAAACCCATCCAAGAACT+TGG | 0.408159 | 7.3:+82122871 | None:intergenic |
| TTCGTGTCCAAGTTCTTGGA+TGG | 0.434485 | 7.3:-82122878 | MS.gene54344:CDS |
| ATACGAAACCCAACACATTT+GGG | 0.439248 | 7.3:+82122332 | None:intergenic |
| TGCTGCTGTATGCAATCTAT+AGG | 0.452278 | 7.3:-82122294 | MS.gene54344:CDS |
| AATAATACCCTTGCATCTCT+TGG | 0.455895 | 7.3:+82123097 | None:intergenic |
| GTACATACCACAATGCTTAG+TGG | 0.491986 | 7.3:+82122819 | None:intergenic |
| GCCACAATGTGGTTTGGCTA+CGG | 0.514248 | 7.3:-82122631 | MS.gene54344:CDS |
| TTGAGTGCCACAATGTGGTT+TGG | 0.514750 | 7.3:-82122637 | MS.gene54344:CDS |
| TCGTGTCCAAGTTCTTGGAT+GGG | 0.519498 | 7.3:-82122877 | MS.gene54344:CDS |
| CTGAACAAAGCTACTAGGTA+AGG | 0.520832 | 7.3:+82123238 | None:intergenic |
| TGGAGCTGAACAAAGCTACT+AGG | 0.521833 | 7.3:+82123233 | None:intergenic |
| CTGCAAAGACACTAACACTA+AGG | 0.526938 | 7.3:+82122844 | None:intergenic |
| GTGCAGCTCCCAAATGTGTT+GGG | 0.530668 | 7.3:-82122340 | MS.gene54344:intron |
| ATGCAATCTATAGGAATGGT+GGG | 0.547366 | 7.3:-82122285 | MS.gene54344:CDS |
| CAAATGAGTTTATGGTAATG+AGG | 0.551806 | 7.3:+82123167 | None:intergenic |
| CATAAACTCATTTGGATGTG+TGG | 0.553344 | 7.3:-82123158 | MS.gene54344:CDS |
| GTTACTTTCGGCAATGAACG+TGG | 0.564481 | 7.3:-82122952 | MS.gene54344:CDS |
| TACAAGAAGAAATCGACAGA+GGG | 0.564739 | 7.3:-82123274 | MS.gene54344:intron |
| GACACGAAGTGGACCATGCA+CGG | 0.579340 | 7.3:+82122893 | None:intergenic |
| TGGCTACGGTCTCTTCCTCA+AGG | 0.581206 | 7.3:-82122617 | MS.gene54344:CDS |
| TGTGCAGCTCCCAAATGTGT+TGG | 0.581637 | 7.3:-82122341 | MS.gene54344:intron |
| GATGTACAATATGATGTAGA+TGG | 0.583280 | 7.3:+82123129 | None:intergenic |
| GTCACTAACTATGCCGTGCA+TGG | 0.586420 | 7.3:-82122906 | MS.gene54344:CDS |
| CCAAGAACTTGGACACGAAG+TGG | 0.588617 | 7.3:+82122882 | None:intergenic |
| CGGCATAGTTAGTGACTATG+AGG | 0.591246 | 7.3:+82122913 | None:intergenic |
| GACTATGAGGATCAAAGCAA+AGG | 0.596059 | 7.3:+82122926 | None:intergenic |
| CTGTATGCAATCTATAGGAA+TGG | 0.607405 | 7.3:-82122289 | MS.gene54344:CDS |
| CTATGCACCAAGAGATGCAA+GGG | 0.607841 | 7.3:-82123104 | MS.gene54344:intron |
| TGCATAGTACAACCAAAGCA+TGG | 0.611574 | 7.3:+82123213 | None:intergenic |
| TATGCAATCTATAGGAATGG+TGG | 0.615800 | 7.3:-82122286 | MS.gene54344:CDS |
| CAGCAATGCATATGTCCTTG+AGG | 0.616655 | 7.3:+82122602 | None:intergenic |
| AGAGTGAATGACAAATTGAA+AGG | 0.622293 | 7.3:+82122664 | None:intergenic |
| TACTTTCGGCAATGAACGTG+GGG | 0.627256 | 7.3:-82122950 | MS.gene54344:CDS |
| TTACTTTCGGCAATGAACGT+GGG | 0.632501 | 7.3:-82122951 | MS.gene54344:CDS |
| TGCAAAGACACTAACACTAA+GGG | 0.634955 | 7.3:+82122845 | None:intergenic |
| TGCAATCTATAGGAATGGTG+GGG | 0.637303 | 7.3:-82122284 | MS.gene54344:CDS |
| TGCAGCACCACTAAGCATTG+TGG | 0.637500 | 7.3:-82122826 | MS.gene54344:intron |
| TCTATGCACCAAGAGATGCA+AGG | 0.675820 | 7.3:-82123105 | MS.gene54344:CDS |
| TCACATTGAGTGCCACAATG+TGG | 0.691357 | 7.3:-82122642 | MS.gene54344:CDS |
| ATACAAGAAGAAATCGACAG+AGG | 0.715649 | 7.3:-82123275 | MS.gene54344:intron |
| ACCGTAGCCAAACCACATTG+TGG | 0.726020 | 7.3:+82122630 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAAAAAATAAAATTAG+TGG | - | chr7.3:82123092-82123111 | MS.gene54344:intron | 10.0% |
| !! | ATTTAAATAAATACTTACAT+TGG | + | chr7.3:82122266-82122285 | None:intergenic | 10.0% |
| !! | CATTCAATAATAAATATTTA+AGG | + | chr7.3:82122661-82122680 | None:intergenic | 10.0% |
| !! | TTATATATATTAATTGAGTA+AGG | + | chr7.3:82122868-82122887 | None:intergenic | 10.0% |
| !! | GATATTAATGTTCAATTAAT+TGG | + | chr7.3:82122608-82122627 | None:intergenic | 15.0% |
| !! | GATTTCATCAAAAAATAATT+AGG | + | chr7.3:82123217-82123236 | None:intergenic | 15.0% |
| !!! | TTTTCTTGAAATTTATATGT+AGG | - | chr7.3:82122928-82122947 | MS.gene54344:CDS | 15.0% |
| !! | AAAACAATCAAATTATGACA+AGG | + | chr7.3:82123141-82123160 | None:intergenic | 20.0% |
| !! | TAATGTTCAATTAATTGGAT+TGG | + | chr7.3:82122603-82122622 | None:intergenic | 20.0% |
| !!! | AGTTTGATTTATGAACTTAA+TGG | - | chr7.3:82122896-82122915 | MS.gene54344:CDS | 20.0% |
| ! | TCGATTTCTTCTTGTATATA+CGG | + | chr7.3:82122372-82122391 | None:intergenic | 25.0% |
| !!! | CTTAACTTTCAAGTTACTTT+CGG | - | chr7.3:82122686-82122705 | MS.gene54344:CDS | 25.0% |
| AGAGTGAATGACAAATTGAA+AGG | + | chr7.3:82122989-82123008 | None:intergenic | 30.0% | |
| CTCATTACCATAAACTCATT+TGG | - | chr7.3:82122484-82122503 | MS.gene54344:intron | 30.0% | |
| GATGTACAATATGATGTAGA+TGG | + | chr7.3:82122524-82122543 | None:intergenic | 30.0% | |
| TGGGGAAAAAACTATGAAAA+AGG | - | chr7.3:82123384-82123403 | MS.gene54344:intron | 30.0% | |
| ! | CAAATGAGTTTATGGTAATG+AGG | + | chr7.3:82122486-82122505 | None:intergenic | 30.0% |
| AAATGTGTTGGGTTTCGTAT+TGG | - | chr7.3:82123321-82123340 | MS.gene54344:intron | 35.0% | |
| AATAATACCCTTGCATCTCT+TGG | + | chr7.3:82122556-82122575 | None:intergenic | 35.0% | |
| AATACGAAACCCAACACATT+TGG | + | chr7.3:82123322-82123341 | None:intergenic | 35.0% | |
| AATGTGTTGGGTTTCGTATT+GGG | - | chr7.3:82123322-82123341 | MS.gene54344:intron | 35.0% | |
| ATACAAGAAGAAATCGACAG+AGG | - | chr7.3:82122375-82122394 | MS.gene54344:intron | 35.0% | |
| ATACGAAACCCAACACATTT+GGG | + | chr7.3:82123321-82123340 | None:intergenic | 35.0% | |
| ATGCAATCTATAGGAATGGT+GGG | - | chr7.3:82123365-82123384 | MS.gene54344:intron | 35.0% | |
| CACACATCCAAATGAGTTTA+TGG | + | chr7.3:82122494-82122513 | None:intergenic | 35.0% | |
| CATAAACTCATTTGGATGTG+TGG | - | chr7.3:82122492-82122511 | MS.gene54344:intron | 35.0% | |
| CTGTATGCAATCTATAGGAA+TGG | - | chr7.3:82123361-82123380 | MS.gene54344:intron | 35.0% | |
| TACAAGAAGAAATCGACAGA+GGG | - | chr7.3:82122376-82122395 | MS.gene54344:intron | 35.0% | |
| TATGCAATCTATAGGAATGG+TGG | - | chr7.3:82123364-82123383 | MS.gene54344:intron | 35.0% | |
| TGCAAAGACACTAACACTAA+GGG | + | chr7.3:82122808-82122827 | None:intergenic | 35.0% | |
| !! | ATACTTTTGAGTGGCTCAAT+TGG | + | chr7.3:82123421-82123440 | None:intergenic | 35.0% |
| CTGAACAAAGCTACTAGGTA+AGG | + | chr7.3:82122415-82122434 | None:intergenic | 40.0% | |
| CTGCAAAGACACTAACACTA+AGG | + | chr7.3:82122809-82122828 | None:intergenic | 40.0% | |
| GACTATGAGGATCAAAGCAA+AGG | + | chr7.3:82122727-82122746 | None:intergenic | 40.0% | |
| GTACATACCACAATGCTTAG+TGG | + | chr7.3:82122834-82122853 | None:intergenic | 40.0% | |
| TGCAATCTATAGGAATGGTG+GGG | - | chr7.3:82123366-82123385 | MS.gene54344:intron | 40.0% | |
| TGCATAGTACAACCAAAGCA+TGG | + | chr7.3:82122440-82122459 | None:intergenic | 40.0% | |
| TGCTGCTGTATGCAATCTAT+AGG | - | chr7.3:82123356-82123375 | MS.gene54344:intron | 40.0% | |
| TTACTTTCGGCAATGAACGT+GGG | - | chr7.3:82122699-82122718 | MS.gene54344:CDS | 40.0% | |
| !! | CATTTCGTTCTTGGTGTTCT+TGG | - | chr7.3:82122238-82122257 | MS.gene54344:CDS | 40.0% |
| CACAAACCCATCCAAGAACT+TGG | + | chr7.3:82122782-82122801 | None:intergenic | 45.0% | |
| CAGCAATGCATATGTCCTTG+AGG | + | chr7.3:82123051-82123070 | None:intergenic | 45.0% | |
| CGGCATAGTTAGTGACTATG+AGG | + | chr7.3:82122740-82122759 | None:intergenic | 45.0% | |
| CTATGCACCAAGAGATGCAA+GGG | - | chr7.3:82122546-82122565 | MS.gene54344:intron | 45.0% | |
| CTTTGTTCAGCTCCATGCTT+TGG | - | chr7.3:82122425-82122444 | MS.gene54344:intron | 45.0% | |
| TACTTTCGGCAATGAACGTG+GGG | - | chr7.3:82122700-82122719 | MS.gene54344:CDS | 45.0% | |
| TCACATTGAGTGCCACAATG+TGG | - | chr7.3:82123008-82123027 | MS.gene54344:intron | 45.0% | |
| TCGTGTCCAAGTTCTTGGAT+GGG | - | chr7.3:82122773-82122792 | MS.gene54344:intron | 45.0% | |
| TCTATGCACCAAGAGATGCA+AGG | - | chr7.3:82122545-82122564 | MS.gene54344:intron | 45.0% | |
| TGGAGCTGAACAAAGCTACT+AGG | + | chr7.3:82122420-82122439 | None:intergenic | 45.0% | |
| TTCGTGTCCAAGTTCTTGGA+TGG | - | chr7.3:82122772-82122791 | MS.gene54344:intron | 45.0% | |
| TTGAGTGCCACAATGTGGTT+TGG | - | chr7.3:82123013-82123032 | MS.gene54344:intron | 45.0% | |
| ! | GTTACTTTCGGCAATGAACG+TGG | - | chr7.3:82122698-82122717 | MS.gene54344:CDS | 45.0% |
| ACCGTAGCCAAACCACATTG+TGG | + | chr7.3:82123023-82123042 | None:intergenic | 50.0% | |
| CCAAGAACTTGGACACGAAG+TGG | + | chr7.3:82122771-82122790 | None:intergenic | 50.0% | |
| CCACTTCGTGTCCAAGTTCT+TGG | - | chr7.3:82122768-82122787 | MS.gene54344:intron | 50.0% | |
| GTCACTAACTATGCCGTGCA+TGG | - | chr7.3:82122744-82122763 | MS.gene54344:intron | 50.0% | |
| GTGCAGCTCCCAAATGTGTT+GGG | - | chr7.3:82123310-82123329 | MS.gene54344:intron | 50.0% | |
| TGTGCAGCTCCCAAATGTGT+TGG | - | chr7.3:82123309-82123328 | MS.gene54344:intron | 50.0% | |
| ! | GCCACAATGTGGTTTGGCTA+CGG | - | chr7.3:82123019-82123038 | MS.gene54344:intron | 50.0% |
| ! | TGCAGCACCACTAAGCATTG+TGG | - | chr7.3:82122824-82122843 | MS.gene54344:intron | 50.0% |
| GACACGAAGTGGACCATGCA+CGG | + | chr7.3:82122760-82122779 | None:intergenic | 55.0% | |
| TGGCTACGGTCTCTTCCTCA+AGG | - | chr7.3:82123033-82123052 | MS.gene54344:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 82122231 | 82123441 | 82122231 | ID=MS.gene54344 |
| chr7.3 | mRNA | 82122231 | 82123441 | 82122231 | ID=MS.gene54344.t1;Parent=MS.gene54344 |
| chr7.3 | exon | 82123413 | 82123441 | 82123413 | ID=MS.gene54344.t1.exon1;Parent=MS.gene54344.t1 |
| chr7.3 | CDS | 82123413 | 82123441 | 82123413 | ID=cds.MS.gene54344.t1;Parent=MS.gene54344.t1 |
| chr7.3 | exon | 82123105 | 82123290 | 82123105 | ID=MS.gene54344.t1.exon2;Parent=MS.gene54344.t1 |
| chr7.3 | CDS | 82123105 | 82123290 | 82123105 | ID=cds.MS.gene54344.t1;Parent=MS.gene54344.t1 |
| chr7.3 | exon | 82122827 | 82122988 | 82122827 | ID=MS.gene54344.t1.exon3;Parent=MS.gene54344.t1 |
| chr7.3 | CDS | 82122827 | 82122988 | 82122827 | ID=cds.MS.gene54344.t1;Parent=MS.gene54344.t1 |
| chr7.3 | exon | 82122603 | 82122722 | 82122603 | ID=MS.gene54344.t1.exon4;Parent=MS.gene54344.t1 |
| chr7.3 | CDS | 82122603 | 82122722 | 82122603 | ID=cds.MS.gene54344.t1;Parent=MS.gene54344.t1 |
| chr7.3 | exon | 82122231 | 82122356 | 82122231 | ID=MS.gene54344.t1.exon5;Parent=MS.gene54344.t1 |
| chr7.3 | CDS | 82122231 | 82122356 | 82122231 | ID=cds.MS.gene54344.t1;Parent=MS.gene54344.t1 |
| Gene Sequence |
| Protein sequence |
