Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54345.t1 | XP_013447780.1 | 92 | 251 | 20 | 0 | 1 | 251 | 17 | 267 | 1.00E-114 | 422.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54345.t1 | P93332 | 90.8 | 251 | 23 | 0 | 1 | 251 | 17 | 267 | 1.8e-115 | 416.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54345.t1 | A0A072TX72 | 92.0 | 251 | 20 | 0 | 1 | 251 | 17 | 267 | 7.3e-115 | 422.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05240 | MS.gene54345 | 0.812061 | 5.12E-51 | -1.69E-46 |
| MS.gene054277 | MS.gene54345 | 0.810753 | 9.85E-51 | -1.69E-46 |
| MS.gene054742 | MS.gene54345 | 0.847722 | 9.66E-60 | -1.69E-46 |
| MS.gene056590 | MS.gene54345 | 0.814388 | 1.58E-51 | -1.69E-46 |
| MS.gene056591 | MS.gene54345 | 0.813536 | 2.44E-51 | -1.69E-46 |
| MS.gene056920 | MS.gene54345 | 0.844813 | 5.99E-59 | -1.69E-46 |
| MS.gene060087 | MS.gene54345 | 0.814595 | 1.42E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 59 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTCGTTCTTGGTGTTCT+TGG | 0.169280 | 7.4:-94248588 | MS.gene54345:CDS |
| CACACATCCAAATGAGTTTA+TGG | 0.188728 | 7.4:+94248336 | None:intergenic |
| CCACTTCGTGTCCAAGTTCT+TGG | 0.244317 | 7.4:-94248060 | MS.gene54345:CDS |
| TCGATTTCTTCTTGTATATA+CGG | 0.244759 | 7.4:+94248458 | None:intergenic |
| CTCATTACCATAAACTCATT+TGG | 0.319248 | 7.4:-94248343 | MS.gene54345:CDS |
| AGGTAACGTCATTTCGTTCT+TGG | 0.323739 | 7.4:-94248597 | MS.gene54345:CDS |
| CTTAACTTTCAAGTTACTTT+CGG | 0.328483 | 7.4:-94248142 | MS.gene54345:CDS |
| AATGTGTTGGGTTTCGTATT+GGG | 0.328786 | 7.4:-94247506 | MS.gene54345:CDS |
| CTTTGTTCAGCTCCATGCTT+TGG | 0.359151 | 7.4:-94248402 | MS.gene54345:CDS |
| AATACGAAACCCAACACATT+TGG | 0.368529 | 7.4:+94247509 | None:intergenic |
| AAATGTGTTGGGTTTCGTAT+TGG | 0.380441 | 7.4:-94247507 | MS.gene54345:CDS |
| CACAAACCCATCCAAGAACT+TGG | 0.408159 | 7.4:+94248049 | None:intergenic |
| ATTTAAACTCCAGAGCCAAT+AGG | 0.432197 | 7.4:+94247302 | None:intergenic |
| TTCGTGTCCAAGTTCTTGGA+TGG | 0.434485 | 7.4:-94248056 | MS.gene54345:CDS |
| ATACGAAACCCAACACATTT+GGG | 0.439248 | 7.4:+94247510 | None:intergenic |
| TGCTGCTGTATGCAATCTAT+AGG | 0.452278 | 7.4:-94247472 | MS.gene54345:CDS |
| AATAATACCCTTGCATCTCT+TGG | 0.455895 | 7.4:+94248274 | None:intergenic |
| GTTCAAAGTGAGGAACCTAT+TGG | 0.474459 | 7.4:-94247317 | MS.gene54345:CDS |
| AGTGAGGAACCTATTGGCTC+TGG | 0.490260 | 7.4:-94247311 | MS.gene54345:CDS |
| GTACATACCACAATGCTTAG+TGG | 0.491986 | 7.4:+94247997 | None:intergenic |
| GCCACAATGTGGTTTGGCTA+CGG | 0.514248 | 7.4:-94247809 | MS.gene54345:CDS |
| TTGAGTGCCACAATGTGGTT+TGG | 0.514750 | 7.4:-94247815 | MS.gene54345:CDS |
| TCGTGTCCAAGTTCTTGGAT+GGG | 0.519498 | 7.4:-94248055 | MS.gene54345:CDS |
| CTGAACAAAGCTACTAGGTA+AGG | 0.520832 | 7.4:+94248415 | None:intergenic |
| TGGAGCTGAACAAAGCTACT+AGG | 0.521833 | 7.4:+94248410 | None:intergenic |
| CTGCAAAGACACTAACACTA+AGG | 0.526938 | 7.4:+94248022 | None:intergenic |
| GTGCAGCTCCCAAATGTGTT+GGG | 0.530668 | 7.4:-94247518 | MS.gene54345:intron |
| GAACAAGGGTGATGATAATG+AGG | 0.540496 | 7.4:-94247345 | MS.gene54345:CDS |
| ATGCAATCTATAGGAATGGT+GGG | 0.547366 | 7.4:-94247463 | MS.gene54345:CDS |
| CAAATGAGTTTATGGTAATG+AGG | 0.551806 | 7.4:+94248344 | None:intergenic |
| CATAAACTCATTTGGATGTG+TGG | 0.553344 | 7.4:-94248335 | MS.gene54345:CDS |
| GTTACTTTCGGCAATGAACG+TGG | 0.564481 | 7.4:-94248130 | MS.gene54345:CDS |
| TACAAGAAGAAATCGACAGA+GGG | 0.564739 | 7.4:-94248451 | MS.gene54345:CDS |
| GACACGAAGTGGACCATGCA+CGG | 0.579340 | 7.4:+94248071 | None:intergenic |
| TGGCTACGGTCTCTTCCTCA+AGG | 0.581206 | 7.4:-94247795 | MS.gene54345:CDS |
| TGTGCAGCTCCCAAATGTGT+TGG | 0.581637 | 7.4:-94247519 | MS.gene54345:intron |
| TATTGTTATAGAGACCCAAT+CGG | 0.581864 | 7.4:-94247390 | MS.gene54345:CDS |
| GATGTACAATATGATGTAGA+TGG | 0.583280 | 7.4:+94248306 | None:intergenic |
| GTCACTAACTATGCCGTGCA+TGG | 0.586420 | 7.4:-94248084 | MS.gene54345:CDS |
| CCAAGAACTTGGACACGAAG+TGG | 0.588617 | 7.4:+94248060 | None:intergenic |
| CGGCATAGTTAGTGACTATG+AGG | 0.591246 | 7.4:+94248091 | None:intergenic |
| GACTATGAGGATCAAAGCAA+AGG | 0.596059 | 7.4:+94248104 | None:intergenic |
| CTGTATGCAATCTATAGGAA+TGG | 0.607405 | 7.4:-94247467 | MS.gene54345:CDS |
| CTATGCACCAAGAGATGCAA+GGG | 0.607841 | 7.4:-94248281 | MS.gene54345:intron |
| TGCATAGTACAACCAAAGCA+TGG | 0.611574 | 7.4:+94248390 | None:intergenic |
| TATGCAATCTATAGGAATGG+TGG | 0.615800 | 7.4:-94247464 | MS.gene54345:CDS |
| CAGCAATGCATATGTCCTTG+AGG | 0.616655 | 7.4:+94247780 | None:intergenic |
| AGAGTGAATGACAAATTGAA+AGG | 0.622293 | 7.4:+94247842 | None:intergenic |
| TACTTTCGGCAATGAACGTG+GGG | 0.627256 | 7.4:-94248128 | MS.gene54345:CDS |
| TTACTTTCGGCAATGAACGT+GGG | 0.632501 | 7.4:-94248129 | MS.gene54345:CDS |
| TGCAAAGACACTAACACTAA+GGG | 0.634955 | 7.4:+94248023 | None:intergenic |
| TGCAATCTATAGGAATGGTG+GGG | 0.637303 | 7.4:-94247462 | MS.gene54345:CDS |
| TGCAGCACCACTAAGCATTG+TGG | 0.637500 | 7.4:-94248004 | MS.gene54345:intron |
| TGAACAAGAAAAGAAGAACA+AGG | 0.638374 | 7.4:-94247360 | MS.gene54345:CDS |
| GAACAAGAAAAGAAGAACAA+GGG | 0.658330 | 7.4:-94247359 | MS.gene54345:CDS |
| TCTATGCACCAAGAGATGCA+AGG | 0.675820 | 7.4:-94248282 | MS.gene54345:CDS |
| TCACATTGAGTGCCACAATG+TGG | 0.691357 | 7.4:-94247820 | MS.gene54345:CDS |
| ATACAAGAAGAAATCGACAG+AGG | 0.715649 | 7.4:-94248452 | MS.gene54345:CDS |
| ACCGTAGCCAAACCACATTG+TGG | 0.726020 | 7.4:+94247808 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAAAAAATAAAATTAG+TGG | - | chr7.4:94248169-94248188 | MS.gene54345:intron | 10.0% |
| !! | ATTTAAATAAATACTTACAT+TGG | + | chr7.4:94247345-94247364 | None:intergenic | 10.0% |
| !! | CATTCAATAATAAATATTTA+AGG | + | chr7.4:94247738-94247757 | None:intergenic | 10.0% |
| !! | TTATATATATTAATTGAGTA+AGG | + | chr7.4:94247945-94247964 | None:intergenic | 10.0% |
| !! | AGATATTAATGTTCAATTAT+TGG | + | chr7.4:94247686-94247705 | None:intergenic | 15.0% |
| !! | GATTTCATCAAAAAATAATT+AGG | + | chr7.4:94248294-94248313 | None:intergenic | 15.0% |
| !!! | TTTTCTTGAAATTTATATGT+AGG | - | chr7.4:94248005-94248024 | MS.gene54345:CDS | 15.0% |
| !! | AAAACAATCAAATTATGACA+AGG | + | chr7.4:94248218-94248237 | None:intergenic | 20.0% |
| !! | TTAATGTTCAATTATTGGAT+TGG | + | chr7.4:94247681-94247700 | None:intergenic | 20.0% |
| !!! | AGTTTGATTTATGAACTTAA+TGG | - | chr7.4:94247973-94247992 | MS.gene54345:intron | 20.0% |
| ! | TCGATTTCTTCTTGTATATA+CGG | + | chr7.4:94247450-94247469 | None:intergenic | 25.0% |
| !! | TCTATAACAATACTTTTGAG+TGG | + | chr7.4:94248507-94248526 | None:intergenic | 25.0% |
| !!! | CTTAACTTTCAAGTTACTTT+CGG | - | chr7.4:94247763-94247782 | MS.gene54345:intron | 25.0% |
| !!! | TTGTTCAATTTTTTCCGATT+GGG | + | chr7.4:94248532-94248551 | None:intergenic | 25.0% |
| AGAGTGAATGACAAATTGAA+AGG | + | chr7.4:94248066-94248085 | None:intergenic | 30.0% | |
| CTCATTACCATAAACTCATT+TGG | - | chr7.4:94247562-94247581 | MS.gene54345:intron | 30.0% | |
| GAACAAGAAAAGAAGAACAA+GGG | - | chr7.4:94248546-94248565 | MS.gene54345:intron | 30.0% | |
| GATGTACAATATGATGTAGA+TGG | + | chr7.4:94247602-94247621 | None:intergenic | 30.0% | |
| TATTGTTATAGAGACCCAAT+CGG | - | chr7.4:94248515-94248534 | MS.gene54345:intron | 30.0% | |
| TGAACAAGAAAAGAAGAACA+AGG | - | chr7.4:94248545-94248564 | MS.gene54345:intron | 30.0% | |
| TGGGGAAAAAACTATGAAAA+AGG | - | chr7.4:94248461-94248480 | MS.gene54345:CDS | 30.0% | |
| ! | CAAATGAGTTTATGGTAATG+AGG | + | chr7.4:94247564-94247583 | None:intergenic | 30.0% |
| !!! | CTTGTTCAATTTTTTCCGAT+TGG | + | chr7.4:94248533-94248552 | None:intergenic | 30.0% |
| AAATGTGTTGGGTTTCGTAT+TGG | - | chr7.4:94248398-94248417 | MS.gene54345:CDS | 35.0% | |
| AATAATACCCTTGCATCTCT+TGG | + | chr7.4:94247634-94247653 | None:intergenic | 35.0% | |
| AATACGAAACCCAACACATT+TGG | + | chr7.4:94248399-94248418 | None:intergenic | 35.0% | |
| AATGTGTTGGGTTTCGTATT+GGG | - | chr7.4:94248399-94248418 | MS.gene54345:CDS | 35.0% | |
| ATACAAGAAGAAATCGACAG+AGG | - | chr7.4:94247453-94247472 | MS.gene54345:CDS | 35.0% | |
| ATACGAAACCCAACACATTT+GGG | + | chr7.4:94248398-94248417 | None:intergenic | 35.0% | |
| ATGCAATCTATAGGAATGGT+GGG | - | chr7.4:94248442-94248461 | MS.gene54345:CDS | 35.0% | |
| CACACATCCAAATGAGTTTA+TGG | + | chr7.4:94247572-94247591 | None:intergenic | 35.0% | |
| CATAAACTCATTTGGATGTG+TGG | - | chr7.4:94247570-94247589 | MS.gene54345:intron | 35.0% | |
| CTGTATGCAATCTATAGGAA+TGG | - | chr7.4:94248438-94248457 | MS.gene54345:CDS | 35.0% | |
| TACAAGAAGAAATCGACAGA+GGG | - | chr7.4:94247454-94247473 | MS.gene54345:CDS | 35.0% | |
| TATGCAATCTATAGGAATGG+TGG | - | chr7.4:94248441-94248460 | MS.gene54345:CDS | 35.0% | |
| TGAGGAAAAAGTTCAAAGTG+AGG | - | chr7.4:94248578-94248597 | MS.gene54345:intron | 35.0% | |
| TGCAAAGACACTAACACTAA+GGG | + | chr7.4:94247885-94247904 | None:intergenic | 35.0% | |
| !! | ATACTTTTGAGTGGCTCAAT+TGG | + | chr7.4:94248498-94248517 | None:intergenic | 35.0% |
| CTGAACAAAGCTACTAGGTA+AGG | + | chr7.4:94247493-94247512 | None:intergenic | 40.0% | |
| CTGCAAAGACACTAACACTA+AGG | + | chr7.4:94247886-94247905 | None:intergenic | 40.0% | |
| GACTATGAGGATCAAAGCAA+AGG | + | chr7.4:94247804-94247823 | None:intergenic | 40.0% | |
| GTACATACCACAATGCTTAG+TGG | + | chr7.4:94247911-94247930 | None:intergenic | 40.0% | |
| GTTCAAAGTGAGGAACCTAT+TGG | - | chr7.4:94248588-94248607 | MS.gene54345:CDS | 40.0% | |
| TGCAATCTATAGGAATGGTG+GGG | - | chr7.4:94248443-94248462 | MS.gene54345:CDS | 40.0% | |
| TGCATAGTACAACCAAAGCA+TGG | + | chr7.4:94247518-94247537 | None:intergenic | 40.0% | |
| TGCTGCTGTATGCAATCTAT+AGG | - | chr7.4:94248433-94248452 | MS.gene54345:CDS | 40.0% | |
| TTACTTTCGGCAATGAACGT+GGG | - | chr7.4:94247776-94247795 | MS.gene54345:intron | 40.0% | |
| ! | GAACAAGGGTGATGATAATG+AGG | - | chr7.4:94248560-94248579 | MS.gene54345:intron | 40.0% |
| !! | CATTTCGTTCTTGGTGTTCT+TGG | - | chr7.4:94247317-94247336 | MS.gene54345:CDS | 40.0% |
| CACAAACCCATCCAAGAACT+TGG | + | chr7.4:94247859-94247878 | None:intergenic | 45.0% | |
| CAGCAATGCATATGTCCTTG+AGG | + | chr7.4:94248128-94248147 | None:intergenic | 45.0% | |
| CGGCATAGTTAGTGACTATG+AGG | + | chr7.4:94247817-94247836 | None:intergenic | 45.0% | |
| CTATGCACCAAGAGATGCAA+GGG | - | chr7.4:94247624-94247643 | MS.gene54345:intron | 45.0% | |
| CTTTGTTCAGCTCCATGCTT+TGG | - | chr7.4:94247503-94247522 | MS.gene54345:CDS | 45.0% | |
| TACTTTCGGCAATGAACGTG+GGG | - | chr7.4:94247777-94247796 | MS.gene54345:intron | 45.0% | |
| TCACATTGAGTGCCACAATG+TGG | - | chr7.4:94248085-94248104 | MS.gene54345:CDS | 45.0% | |
| TCGTGTCCAAGTTCTTGGAT+GGG | - | chr7.4:94247850-94247869 | MS.gene54345:CDS | 45.0% | |
| TCTATGCACCAAGAGATGCA+AGG | - | chr7.4:94247623-94247642 | MS.gene54345:intron | 45.0% | |
| TGGAGCTGAACAAAGCTACT+AGG | + | chr7.4:94247498-94247517 | None:intergenic | 45.0% | |
| TTCGTGTCCAAGTTCTTGGA+TGG | - | chr7.4:94247849-94247868 | MS.gene54345:CDS | 45.0% | |
| TTGAGTGCCACAATGTGGTT+TGG | - | chr7.4:94248090-94248109 | MS.gene54345:CDS | 45.0% | |
| ! | GTTACTTTCGGCAATGAACG+TGG | - | chr7.4:94247775-94247794 | MS.gene54345:intron | 45.0% |
| ACCGTAGCCAAACCACATTG+TGG | + | chr7.4:94248100-94248119 | None:intergenic | 50.0% | |
| CCAAGAACTTGGACACGAAG+TGG | + | chr7.4:94247848-94247867 | None:intergenic | 50.0% | |
| CCACTTCGTGTCCAAGTTCT+TGG | - | chr7.4:94247845-94247864 | MS.gene54345:CDS | 50.0% | |
| GTCACTAACTATGCCGTGCA+TGG | - | chr7.4:94247821-94247840 | MS.gene54345:CDS | 50.0% | |
| GTGCAGCTCCCAAATGTGTT+GGG | - | chr7.4:94248387-94248406 | MS.gene54345:CDS | 50.0% | |
| TGTGCAGCTCCCAAATGTGT+TGG | - | chr7.4:94248386-94248405 | MS.gene54345:CDS | 50.0% | |
| ! | GCCACAATGTGGTTTGGCTA+CGG | - | chr7.4:94248096-94248115 | MS.gene54345:CDS | 50.0% |
| ! | TGCAGCACCACTAAGCATTG+TGG | - | chr7.4:94247901-94247920 | MS.gene54345:intron | 50.0% |
| !! | AGTGAGGAACCTATTGGCTC+TGG | - | chr7.4:94248594-94248613 | MS.gene54345:CDS | 50.0% |
| GACACGAAGTGGACCATGCA+CGG | + | chr7.4:94247837-94247856 | None:intergenic | 55.0% | |
| TGGCTACGGTCTCTTCCTCA+AGG | - | chr7.4:94248110-94248129 | MS.gene54345:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 94247310 | 94248617 | 94247310 | ID=MS.gene54345 |
| chr7.4 | mRNA | 94247310 | 94248617 | 94247310 | ID=MS.gene54345.t1;Parent=MS.gene54345 |
| chr7.4 | exon | 94248581 | 94248617 | 94248581 | ID=MS.gene54345.t1.exon1;Parent=MS.gene54345.t1 |
| chr7.4 | CDS | 94248581 | 94248617 | 94248581 | ID=cds.MS.gene54345.t1;Parent=MS.gene54345.t1 |
| chr7.4 | exon | 94248282 | 94248492 | 94248282 | ID=MS.gene54345.t1.exon2;Parent=MS.gene54345.t1 |
| chr7.4 | CDS | 94248282 | 94248492 | 94248282 | ID=cds.MS.gene54345.t1;Parent=MS.gene54345.t1 |
| chr7.4 | exon | 94248005 | 94248166 | 94248005 | ID=MS.gene54345.t1.exon3;Parent=MS.gene54345.t1 |
| chr7.4 | CDS | 94248005 | 94248166 | 94248005 | ID=cds.MS.gene54345.t1;Parent=MS.gene54345.t1 |
| chr7.4 | exon | 94247781 | 94247900 | 94247781 | ID=MS.gene54345.t1.exon4;Parent=MS.gene54345.t1 |
| chr7.4 | CDS | 94247781 | 94247900 | 94247781 | ID=cds.MS.gene54345.t1;Parent=MS.gene54345.t1 |
| chr7.4 | exon | 94247310 | 94247534 | 94247310 | ID=MS.gene54345.t1.exon5;Parent=MS.gene54345.t1 |
| chr7.4 | CDS | 94247310 | 94247534 | 94247310 | ID=cds.MS.gene54345.t1;Parent=MS.gene54345.t1 |
| Gene Sequence |
| Protein sequence |
