AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54375

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54375.t1 MTR_7g006030 100.000 599 0 0 1 599 1 599 0.0 1258
MS.gene54375.t1 MTR_1g116210 85.572 603 78 2 1 599 79 676 0.0 1105
MS.gene54375.t1 MTR_1g007150 78.869 601 116 3 3 598 89 683 0.0 1019
MS.gene54375.t1 MTR_7g085540 79.595 593 120 1 7 599 117 708 0.0 1018
MS.gene54375.t1 MTR_3g110240 77.796 599 123 2 4 598 88 680 0.0 1013
MS.gene54375.t1 MTR_8g014050 72.987 596 158 3 6 599 97 691 0.0 921
MS.gene54375.t1 MTR_1g042280 39.100 289 157 6 31 316 6 278 5.99e-60 207
MS.gene54375.t1 MTR_2g099670 38.710 279 154 5 37 312 9 273 8.99e-60 207
MS.gene54375.t1 MTR_4g126010 38.351 279 155 5 37 312 9 273 1.04e-59 206
MS.gene54375.t1 MTR_2g039930 37.993 279 156 5 37 312 9 273 2.89e-59 205
MS.gene54375.t1 MTR_1g075870 38.754 289 158 6 31 316 6 278 3.12e-59 201
MS.gene54375.t1 MTR_7g097160 38.516 283 157 5 37 316 9 277 7.10e-59 204
MS.gene54375.t1 MTR_1g075870 38.754 289 158 6 31 316 6 278 4.94e-58 202
MS.gene54375.t1 MTR_1g069720 37.716 289 161 6 31 316 5 277 1.98e-57 200
MS.gene54375.t1 MTR_8g018310 37.276 279 157 6 37 312 9 272 8.62e-57 199
MS.gene54375.t1 MTR_8g018310 36.749 283 161 6 37 316 9 276 1.09e-56 196
MS.gene54375.t1 MTR_6g087070 36.268 284 164 5 37 317 9 278 2.10e-55 193
MS.gene54375.t1 MTR_8g102930 36.735 294 166 7 27 317 2 278 7.07e-55 192
MS.gene54375.t1 MTR_1g042280 41.304 230 129 3 90 316 20 246 3.42e-54 187
MS.gene54375.t1 MTR_0492s0030 36.860 293 164 6 29 316 3 279 5.60e-54 188
MS.gene54375.t1 MTR_0543s0020 36.860 293 164 6 29 316 3 279 5.60e-54 188
MS.gene54375.t1 MTR_1g004990 35.439 285 165 6 31 312 5 273 8.08e-53 186
MS.gene54375.t1 MTR_3g110180 35.088 285 166 6 31 312 5 273 1.14e-52 186
MS.gene54375.t1 MTR_3g110180 35.088 285 165 7 31 312 5 272 3.25e-51 182
MS.gene54375.t1 MTR_2g099670 41.784 213 118 3 103 312 12 221 1.06e-50 181
MS.gene54375.t1 MTR_1g075870 40.541 222 126 3 98 316 3 221 2.36e-50 176
MS.gene54375.t1 MTR_2g039930 39.487 195 112 3 121 312 1 192 4.73e-43 159
MS.gene54375.t1 MTR_1g116205 73.737 99 17 2 1 95 79 172 1.50e-41 148
MS.gene54375.t1 MTR_7g097160 41.481 135 75 2 185 316 25 158 1.22e-27 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54375.t1 AT3G13670 85.050 602 84 2 1 598 103 702 0.0 1098
MS.gene54375.t1 AT2G25760 77.537 601 126 4 3 598 76 672 0.0 1011
MS.gene54375.t1 AT2G25760 77.318 604 125 6 3 598 76 675 0.0 1004
MS.gene54375.t1 AT5G18190 76.897 593 136 1 7 599 100 691 0.0 994
MS.gene54375.t1 AT5G18190 76.897 593 136 1 7 599 100 691 0.0 994
MS.gene54375.t1 AT3G03940 76.728 593 137 1 7 599 110 701 0.0 994
MS.gene54375.t1 AT3G03930 65.660 265 82 2 343 598 23 287 4.74e-124 367
MS.gene54375.t1 AT5G44100 38.408 289 159 6 31 316 5 277 1.43e-59 206
MS.gene54375.t1 AT5G44100 38.408 289 159 6 31 316 5 277 1.43e-59 206
MS.gene54375.t1 AT2G19470 38.516 283 157 5 37 316 9 277 1.59e-59 205
MS.gene54375.t1 AT1G03930 38.596 285 156 6 31 312 5 273 1.72e-59 206
MS.gene54375.t1 AT3G23340 38.754 289 158 6 31 316 5 277 4.49e-59 204
MS.gene54375.t1 AT4G14340 38.408 289 159 6 31 316 11 283 1.21e-58 201
MS.gene54375.t1 AT4G28540 38.062 289 160 6 31 316 9 281 1.50e-58 203
MS.gene54375.t1 AT4G26100 36.749 283 162 5 37 316 9 277 3.59e-58 202
MS.gene54375.t1 AT4G14340 38.408 289 159 6 31 316 11 283 3.70e-58 202
MS.gene54375.t1 AT4G28860 37.543 293 163 7 27 316 2 277 3.63e-57 197
MS.gene54375.t1 AT4G28860 37.543 293 163 7 27 316 2 277 1.44e-56 196
MS.gene54375.t1 AT1G04440 38.062 289 160 6 31 316 5 277 1.52e-56 198
MS.gene54375.t1 AT5G43320 38.062 289 160 6 31 316 5 277 1.77e-56 198
MS.gene54375.t1 AT4G28880 37.884 293 162 7 27 316 2 277 1.82e-56 196
MS.gene54375.t1 AT4G28880 37.884 293 162 7 27 316 2 277 1.82e-56 196
MS.gene54375.t1 AT4G28880 37.884 293 162 7 27 316 2 277 1.82e-56 196
MS.gene54375.t1 AT4G28880 37.884 293 162 7 27 316 2 277 1.82e-56 196
MS.gene54375.t1 AT1G72710 37.276 279 157 6 37 312 9 272 1.98e-56 197
MS.gene54375.t1 AT5G57015 36.042 283 164 5 37 316 9 277 2.81e-56 196
MS.gene54375.t1 AT5G43320 38.062 289 160 6 31 316 5 277 2.91e-56 194
MS.gene54375.t1 AT5G43320 38.062 289 160 6 31 316 5 277 2.91e-56 194
MS.gene54375.t1 AT3G23340 39.245 265 151 5 57 316 4 263 1.37e-53 189
MS.gene54375.t1 AT4G08800 32.509 283 142 6 37 316 9 245 2.53e-42 154

Find 165 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCTCTGGGCACTTATTA+TGG 0.176547 7.4:-93660281 MS.gene54375:CDS
CATCTCTACCTTTGCTTCAT+TGG 0.235235 7.4:+93661105 None:intergenic
TGGCTTCTTGTTCCAATCTC+TGG 0.237696 7.4:-93660294 MS.gene54375:CDS
AAAAGAAACCTGAACATATC+AGG 0.258582 7.4:+93661928 None:intergenic
TGAGCACCTTCAGTATTAAT+TGG 0.274374 7.4:+93661365 None:intergenic
AAACTTGGCAAAGGTGGATT+TGG 0.289497 7.4:-93663642 MS.gene54375:CDS
CTTCCTTTATAATGTACTTT+GGG 0.297099 7.4:+93663229 None:intergenic
TGCAAGTGTTCATGCTCATT+TGG 0.304706 7.4:-93661766 MS.gene54375:CDS
GGTGGTAATGACCGTCTTAA+TGG 0.307348 7.4:-93663591 MS.gene54375:CDS
TTTGTTGGTCGTCGAGTTAC+CGG 0.308181 7.4:-93663612 MS.gene54375:CDS
AACTCAAGGAAATGTCTAAA+AGG 0.324752 7.4:+93661479 None:intergenic
GTTACCATGGCAAGGTTATC+AGG 0.332076 7.4:-93661673 MS.gene54375:intron
AACCATTATCCCATCGTCTA+TGG 0.333296 7.4:+93657703 None:intergenic
TTTCTTGTTGATCTCGGATT+AGG 0.333361 7.4:-93662092 MS.gene54375:intron
GTCCAAGCTTGTGGGATGTT+TGG 0.336979 7.4:-93663076 MS.gene54375:CDS
TCTATAGCTGGGGTTACTAA+TGG 0.339143 7.4:-93660061 MS.gene54375:CDS
CTTATTATGGATGCTGGAAC+TGG 0.352603 7.4:-93660268 MS.gene54375:CDS
GGCTTCTTGTTCCAATCTCT+GGG 0.354817 7.4:-93660293 MS.gene54375:CDS
TTCTTCTCTTGTGCTGTACC+TGG 0.364237 7.4:+93662119 None:intergenic
ATTGGCCGCAGTGCAGGATT+TGG 0.366177 7.4:+93661383 None:intergenic
TACCTTGCTTGCTTGTGCTA+TGG 0.369695 7.4:-93657448 MS.gene54375:CDS
CATAATAAGTGCCCAGAGAT+TGG 0.371273 7.4:+93660282 None:intergenic
TCTTCCTTTATAATGTACTT+TGG 0.373082 7.4:+93663228 None:intergenic
GACGATGGGATAATGGTTAC+AGG 0.373311 7.4:-93657698 MS.gene54375:CDS
AGCAATGCATGCCACCATTT+CGG 0.376237 7.4:+93662332 None:intergenic
ATTTCCAAGCACACATGTTA+AGG 0.378927 7.4:-93657574 MS.gene54375:intron
GGCCACTGCTGGAAGTCGTT+GGG 0.383031 7.4:-93658772 MS.gene54375:CDS
ACCTTGCTTGCTTGTGCTAT+GGG 0.388384 7.4:-93657447 MS.gene54375:CDS
GCAAGTGTTCATGCTCATTT+GGG 0.388698 7.4:-93661765 MS.gene54375:CDS
CAGAAGCAGCATAGCATCTC+AGG 0.394079 7.4:+93661509 None:intergenic
CTTCTTGTTTATCCATTTGA+AGG 0.400317 7.4:+93658824 None:intergenic
TATTACATTACTTCTATAGC+TGG 0.401862 7.4:-93660073 MS.gene54375:CDS
ATCCATAGACGATGGGATAA+TGG 0.409318 7.4:-93657705 MS.gene54375:CDS
ATACCCAAAGTACATTATAA+AGG 0.411632 7.4:-93663232 MS.gene54375:CDS
GATATTAGCTTGGAATAGTT+AGG 0.422192 7.4:+93661428 None:intergenic
ATTGATATCCATTGTGTAGC+AGG 0.425137 7.4:+93661144 None:intergenic
ACACATACCCTCTCAGGAAA+AGG 0.427758 7.4:+93664622 None:intergenic
GATAACCTTGCCATGGTAAC+CGG 0.435532 7.4:+93661676 None:intergenic
CTATAGCTGGGGTTACTAAT+GGG 0.437207 7.4:-93660060 MS.gene54375:CDS
ACCAGCGCCCTGATATGTTC+AGG 0.437919 7.4:-93661936 MS.gene54375:intron
TTCAAAGTGGAGAGGAAACT+TGG 0.443586 7.4:-93663657 MS.gene54375:CDS
GCGAGTCTTTCCCCTTCAAA+TGG 0.445977 7.4:-93658836 MS.gene54375:CDS
CACATACCCTCTCAGGAAAA+GGG 0.446418 7.4:+93664623 None:intergenic
CGGCATACCTCAGTGGCACC+GGG 0.450194 7.4:+93663567 None:intergenic
GTATTAATTGGCCGCAGTGC+AGG 0.451258 7.4:+93661377 None:intergenic
TCTCTTGTGGTGATGTCAAA+AGG 0.451724 7.4:-93660034 MS.gene54375:intron
TCGGCATACCTCAGTGGCAC+CGG 0.452841 7.4:+93663566 None:intergenic
GTTTCCTGAGTTTCGTCAGC+AGG 0.453762 7.4:+93657612 None:intergenic
TAATACTGAAGGTGCTCAAA+AGG 0.460450 7.4:-93661360 MS.gene54375:intron
TCCTTGTTTGATAGTGTGCT+TGG 0.461993 7.4:-93661407 MS.gene54375:CDS
TGTGGAGTCATTGTCGATCT+TGG 0.469974 7.4:-93662310 MS.gene54375:CDS
TTCATTGTAGGTATGTGCAT+GGG 0.470805 7.4:-93662175 MS.gene54375:intron
AAATTGTTTCTTGTTGATCT+CGG 0.470903 7.4:-93662098 MS.gene54375:CDS
GTGACTTCAATGGCCACTGC+TGG 0.476110 7.4:-93658783 MS.gene54375:CDS
ATGTAGGATGTCTGCCGAAA+TGG 0.477227 7.4:-93662346 MS.gene54375:intron
ATTACATTACTTCTATAGCT+GGG 0.477779 7.4:-93660072 MS.gene54375:CDS
GGAATGGATCATGGAGCAAT+GGG 0.480666 7.4:-93660104 MS.gene54375:CDS
GTTGTTATGTCACGGAATGC+TGG 0.481715 7.4:-93658747 MS.gene54375:CDS
GAGAGGTAGATTGAATATTG+AGG 0.484728 7.4:-93661211 MS.gene54375:CDS
AGGGTTTCACGTGACTTCAA+TGG 0.485148 7.4:-93658793 MS.gene54375:CDS
TGGCAAAGGTGGATTTGGAC+AGG 0.492641 7.4:-93663637 MS.gene54375:CDS
TTCCAAACATCCCACAAGCT+TGG 0.493712 7.4:+93663074 None:intergenic
CATAGGACACATACCCTCTC+AGG 0.494559 7.4:+93664616 None:intergenic
AACAGTAAAGGTTGCAACTA+CGG 0.495338 7.4:-93663441 MS.gene54375:CDS
CGTCTATGGATTCCCTCGCT+AGG 0.500795 7.4:+93657717 None:intergenic
AGGAAATGTCTAAAAGGAGT+AGG 0.502905 7.4:+93661485 None:intergenic
ATCAAACAAGGATATTAGCT+TGG 0.504809 7.4:+93661418 None:intergenic
TGGCCACTGCTGGAAGTCGT+TGG 0.506701 7.4:-93658773 MS.gene54375:CDS
GAATGCTGGATTCAGTGATC+AGG 0.508380 7.4:-93658733 MS.gene54375:intron
CATGCTTGGTCCAAGCTTGT+GGG 0.512086 7.4:-93663084 MS.gene54375:CDS
TTCTTGTTTATCCATTTGAA+GGG 0.516197 7.4:+93658825 None:intergenic
TTCCTCTCCACTTTGAACAT+AGG 0.519828 7.4:+93663663 None:intergenic
AGCACCCTTGGTGGAAGTCA+TGG 0.521686 7.4:-93663256 MS.gene54375:CDS
TAAACAAGAAGTGGAGAGAA+GGG 0.522540 7.4:-93658812 MS.gene54375:CDS
TGGGCACTTATTATGGATGC+TGG 0.523125 7.4:-93660274 MS.gene54375:CDS
TCACAGCAACTGCAGCTACA+TGG 0.524987 7.4:-93657674 MS.gene54375:CDS
AGGTGTTGCTCAAGAGGATG+AGG 0.525431 7.4:-93664656 MS.gene54375:CDS
AGCACAAGCAAGCAAGGTAA+AGG 0.526556 7.4:+93657452 None:intergenic
AAGTGGAGAGACACCTCCAC+TGG 0.527530 7.4:-93661976 MS.gene54375:CDS
TCAAATGGATAAACAAGAAG+TGG 0.528244 7.4:-93658821 MS.gene54375:CDS
TTCTTTCTAGGAATGGATCA+TGG 0.532786 7.4:-93660113 MS.gene54375:intron
GTTCATTGTAGGTATGTGCA+TGG 0.539598 7.4:-93662176 MS.gene54375:intron
CAAACCTTAACATGTGTGCT+TGG 0.539899 7.4:+93657570 None:intergenic
ACCTGAACATATCAGGGCGC+TGG 0.539965 7.4:+93661935 None:intergenic
GGTGGTTTGAGTGCTAATAA+AGG 0.540456 7.4:-93664676 MS.gene54375:CDS
TAATAAAGGTGTTGCTCAAG+AGG 0.543294 7.4:-93664662 MS.gene54375:CDS
TACTAATGGGAGCTCTCTTG+TGG 0.544290 7.4:-93660047 MS.gene54375:CDS
TTCATAAAGGCCGGTTACCA+TGG 0.544662 7.4:-93661686 MS.gene54375:CDS
ATGCAGGTTATGGACATGCT+TGG 0.546212 7.4:-93663098 MS.gene54375:intron
ACGAAACTCAGGAAACTCTT+CGG 0.546872 7.4:-93657605 MS.gene54375:CDS
GTCTATGGATTCCCTCGCTA+GGG 0.547554 7.4:+93657718 None:intergenic
GACCCAAAGGTTCAAGTAGG+AGG 0.548405 7.4:-93663690 MS.gene54375:intron
ATAAACAAGAAGTGGAGAGA+AGG 0.549123 7.4:-93658813 MS.gene54375:CDS
GGATTCCCTCGCTAGGGTAG+AGG 0.550492 7.4:+93657724 None:intergenic
TTTGAACATAGAAACAGTAA+AGG 0.551586 7.4:-93663453 MS.gene54375:CDS
AGGAATGGATCATGGAGCAA+TGG 0.552979 7.4:-93660105 MS.gene54375:CDS
GGAAATGTCTAAAAGGAGTA+GGG 0.553070 7.4:+93661486 None:intergenic
TGGGATGTTTGGAATACCAC+AGG 0.555511 7.4:-93663065 MS.gene54375:CDS
AAGACCTGCTGACGAAACTC+AGG 0.556055 7.4:-93657616 MS.gene54375:CDS
TCTTGGAGAAGATGCACTCA+AGG 0.557515 7.4:-93662293 MS.gene54375:CDS
CACAGCAACTGCAGCTACAT+GGG 0.559415 7.4:-93657673 MS.gene54375:CDS
GAGAGGAAACTTGGCAAAGG+TGG 0.562906 7.4:-93663648 MS.gene54375:CDS
ACATGCTTGGTCCAAGCTTG+TGG 0.566277 7.4:-93663085 MS.gene54375:CDS
TCGTCAGCAGGTCTTCGCCT+CGG 0.568146 7.4:+93657624 None:intergenic
AAGCCACACAGCTAATAAGA+AGG 0.568966 7.4:+93660311 None:intergenic
TTGTACACTTGCCACTCGTA+AGG 0.569716 7.4:+93663414 None:intergenic
AATGACCGTCTTAATGGCCC+CGG 0.574417 7.4:-93663585 MS.gene54375:CDS
TACACTTGCCACTCGTAAGG+AGG 0.576490 7.4:+93663417 None:intergenic
TTTGACCCAAAGGTTCAAGT+AGG 0.576801 7.4:-93663693 MS.gene54375:intron
TGTTTGGAATACCACAGGCC+AGG 0.580670 7.4:-93663060 MS.gene54375:CDS
GTTGGGGTGTTGTTATGTCA+CGG 0.581602 7.4:-93658755 MS.gene54375:CDS
TCATGTTCACTACAAACTCA+AGG 0.582669 7.4:+93661465 None:intergenic
GCCCATAGCACAAGCAAGCA+AGG 0.584968 7.4:+93657446 None:intergenic
GGGGTGATGGGTGATGAAAG+TGG 0.587284 7.4:-93664697 None:intergenic
TAATGGCCCCGGTGCCACTG+AGG 0.587700 7.4:-93663574 MS.gene54375:intron
TATCATTACAATGTAGCGGA+TGG 0.588065 7.4:-93660373 MS.gene54375:CDS
TGGCACCGGGGCCATTAAGA+CGG 0.592504 7.4:+93663580 None:intergenic
TTCAACATGCTGCCCAGTGG+AGG 0.595156 7.4:+93661963 None:intergenic
CAAGAATGCCAATGAAGCAA+AGG 0.595613 7.4:-93661113 MS.gene54375:intron
CTTGGAGAAGATGCACTCAA+GGG 0.598220 7.4:-93662292 MS.gene54375:CDS
CGGCCTTCTTATTAGCTGTG+TGG 0.598415 7.4:-93660314 MS.gene54375:CDS
CTGCGGCCAATTAATACTGA+AGG 0.599700 7.4:-93661371 MS.gene54375:CDS
CGAGGGAATCCATAGACGAT+GGG 0.602040 7.4:-93657712 MS.gene54375:CDS
AAAGAAACCTGAACATATCA+GGG 0.602522 7.4:+93661929 None:intergenic
TACCAGGTCGGGCAAAAGAG+AGG 0.609022 7.4:-93661228 MS.gene54375:intron
ATTAAGACGGTCATTACCAC+CGG 0.611458 7.4:+93663593 None:intergenic
GCGAGGGAATCCATAGACGA+TGG 0.611468 7.4:-93657713 MS.gene54375:CDS
GATTTCCTCTACCCTAGCGA+GGG 0.615108 7.4:-93657729 MS.gene54375:CDS
TCTTGTTTATCCATTTGAAG+GGG 0.615159 7.4:+93658826 None:intergenic
TGATTTCCTCTACCCTAGCG+AGG 0.615622 7.4:-93657730 MS.gene54375:CDS
CGCCTATGTTCAAAGTGGAG+AGG 0.617102 7.4:-93663665 MS.gene54375:CDS
ACCCCAACGACTTCCAGCAG+TGG 0.622356 7.4:+93658770 None:intergenic
TTATACTCACGCCTGGCCTG+TGG 0.625190 7.4:+93663049 None:intergenic
TCATTGTAGGTATGTGCATG+GGG 0.626331 7.4:-93662174 MS.gene54375:intron
TTACATTACTTCTATAGCTG+GGG 0.629898 7.4:-93660071 MS.gene54375:CDS
TATTCCATGACTTCCACCAA+GGG 0.633471 7.4:+93663252 None:intergenic
ACATACCCTCTCAGGAAAAG+GGG 0.634258 7.4:+93664624 None:intergenic
AGCACCTGATAACCTTGCCA+TGG 0.635229 7.4:+93661669 None:intergenic
TTGGAGAAGATGCACTCAAG+GGG 0.638893 7.4:-93662291 MS.gene54375:CDS
AAAGGCCGGTTACCATGGCA+AGG 0.640441 7.4:-93661681 MS.gene54375:CDS
ACACTTGCCACTCGTAAGGA+GGG 0.641152 7.4:+93663418 None:intergenic
ACAAGGAGAATATTACGTGA+TGG 0.641400 7.4:-93663206 MS.gene54375:intron
TAGCGCAACATGTAGAGCGA+GGG 0.645395 7.4:-93660345 MS.gene54375:CDS
GCCACTGCTGGAAGTCGTTG+GGG 0.646395 7.4:-93658771 MS.gene54375:CDS
GTAGAGCGAGGGATTGCAGA+CGG 0.650118 7.4:-93660334 MS.gene54375:CDS
AGTGGAGAGACACCTCCACT+GGG 0.650761 7.4:-93661975 MS.gene54375:CDS
TCCAAGCACACTATCAAACA+AGG 0.651722 7.4:+93661406 None:intergenic
GTGGAGAGGAAACTTGGCAA+AGG 0.654106 7.4:-93663651 MS.gene54375:CDS
TAGGATGTCTGCCGAAATGG+TGG 0.657696 7.4:-93662343 MS.gene54375:CDS
CTAGCGCAACATGTAGAGCG+AGG 0.658446 7.4:-93660346 MS.gene54375:CDS
GTACATTATAAAGGAAGACA+AGG 0.658821 7.4:-93663223 MS.gene54375:CDS
GTTGGTCGTCGAGTTACCGG+TGG 0.658919 7.4:-93663609 MS.gene54375:CDS
GGTGTTGCTCAAGAGGATGA+GGG 0.659331 7.4:-93664655 MS.gene54375:CDS
TAGGTATCATTACAATGTAG+CGG 0.659418 7.4:-93660377 MS.gene54375:CDS
AATGGTGGCATGCATTGCTG+TGG 0.668208 7.4:-93662328 MS.gene54375:CDS
CTCTTATATTAAGCAAACCG+AGG 0.669496 7.4:-93657641 MS.gene54375:CDS
GTATTCCATGACTTCCACCA+AGG 0.669569 7.4:+93663251 None:intergenic
AGGTTCGCCTATGTTCAAAG+TGG 0.676871 7.4:-93663670 MS.gene54375:CDS
ATATTCAACATGCTGCCCAG+TGG 0.679289 7.4:+93661960 None:intergenic
CAACCCTCGGCATACCTCAG+TGG 0.689534 7.4:+93663560 None:intergenic
ACTACGGCCCTCCTTACGAG+TGG 0.697469 7.4:-93663425 MS.gene54375:CDS
GGCATACCTCAGTGGCACCG+GGG 0.710860 7.4:+93663568 None:intergenic
TGGAGAAGATGCACTCAAGG+GGG 0.711646 7.4:-93662290 MS.gene54375:intron
GTGATGGGTGATGAAAGTGG+TGG 0.743174 7.4:-93664694 MS.gene54375:CDS
TTGGACCAAATCCTGCACTG+CGG 0.818000 7.4:-93661388 MS.gene54375:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.4 gene 93657432 93664713 93657432 ID=MS.gene54375
chr7.4 mRNA 93657432 93664713 93657432 ID=MS.gene54375.t1;Parent=MS.gene54375
chr7.4 exon 93664630 93664713 93664630 ID=MS.gene54375.t1.exon1;Parent=MS.gene54375.t1
chr7.4 CDS 93664630 93664713 93664630 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93663575 93663703 93663575 ID=MS.gene54375.t1.exon2;Parent=MS.gene54375.t1
chr7.4 CDS 93663575 93663703 93663575 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93663414 93663487 93663414 ID=MS.gene54375.t1.exon3;Parent=MS.gene54375.t1
chr7.4 CDS 93663414 93663487 93663414 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93663207 93663276 93663207 ID=MS.gene54375.t1.exon4;Parent=MS.gene54375.t1
chr7.4 CDS 93663207 93663276 93663207 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93663059 93663114 93663059 ID=MS.gene54375.t1.exon5;Parent=MS.gene54375.t1
chr7.4 CDS 93663059 93663114 93663059 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93662291 93662362 93662291 ID=MS.gene54375.t1.exon6;Parent=MS.gene54375.t1
chr7.4 CDS 93662291 93662362 93662291 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93662093 93662187 93662093 ID=MS.gene54375.t1.exon7;Parent=MS.gene54375.t1
chr7.4 CDS 93662093 93662187 93662093 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93661937 93662003 93661937 ID=MS.gene54375.t1.exon8;Parent=MS.gene54375.t1
chr7.4 CDS 93661937 93662003 93661937 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93661674 93661803 93661674 ID=MS.gene54375.t1.exon9;Parent=MS.gene54375.t1
chr7.4 CDS 93661674 93661803 93661674 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93661361 93661579 93661361 ID=MS.gene54375.t1.exon10;Parent=MS.gene54375.t1
chr7.4 CDS 93661361 93661579 93661361 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93661114 93661244 93661114 ID=MS.gene54375.t1.exon11;Parent=MS.gene54375.t1
chr7.4 CDS 93661114 93661244 93661114 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93660222 93660396 93660222 ID=MS.gene54375.t1.exon12;Parent=MS.gene54375.t1
chr7.4 CDS 93660222 93660396 93660222 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93660035 93660125 93660035 ID=MS.gene54375.t1.exon13;Parent=MS.gene54375.t1
chr7.4 CDS 93660035 93660125 93660035 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93658734 93658891 93658734 ID=MS.gene54375.t1.exon14;Parent=MS.gene54375.t1
chr7.4 CDS 93658734 93658891 93658734 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93657575 93657763 93657575 ID=MS.gene54375.t1.exon15;Parent=MS.gene54375.t1
chr7.4 CDS 93657575 93657763 93657575 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
chr7.4 exon 93657432 93657491 93657432 ID=MS.gene54375.t1.exon16;Parent=MS.gene54375.t1
chr7.4 CDS 93657432 93657491 93657432 ID=cds.MS.gene54375.t1;Parent=MS.gene54375.t1
Gene Sequence

>MS.gene54375

ATGGGTGATGAAAGTGGTGGTTTGAGTGCTAATAAAGGTGTTGCTCAAGAGGATGAGGGCAATACTACCCCTTTTCCTGAGAGGGTATGTGTCCTATGAGTTTGTTCAATTTTTCATGGCATGAAAATGCTTTAGATCATGTTTTTTTTTTTTTTTGGTTGGTTTTTATGAGCTAGCCGTGAAATTAACACACTAAATGCAGAGAAGTAGGGATAAATGGGCTGCGTTCCAGCATATCAATTGTTCCTACAACATGTAGTCAAGAGAGTTTGGATTATGTTTTAATTAGAATGAATTTATTATTTATGGTAACTTGATTTTGATCGGATAAATGTTGGTATAGATTTATTGATATCTATGGAGAAGTTTAGAAATCGGATAAATGTTGGTATAGATTTATTGATATCTATGGAGAAGTTTAGAAAGTTAGCTCATCGAAAAAAAATAATTTTCATCTTATACAAAGAAACCATGTTTTAAAAAATTTATTTATGTTGCAAAGATTGTTTCTGCAACCAATTTGATTGATAAACACCTAATTATGTTTGGTTAAGTTTATAAAAATATGTAATCAGTAGGTGCAATCATACAAGCACTAATGCATCGGATCCCATTAGAATTTCATAGTAAAGAGATGGCCTGAACAAGTGTTCATAAGTGGGGGCAATCCTCACCTCACAACCGGTTTTTTGGGGTTGTTTTAGGGGCGAAACTAGAACTAGGATGGGCGACCTCTTGGGTTGCACCTCATCCCAACACTTTTGGAACCCAAAAAATATGCAATGAATTATGTCTTACTAATAAGCTCAAAAGGAGCTTAGTTTAAGAAGATTTTTGTATATGCGATGGTTAATTAAGCTTTTATGAATTAAGCTGTTTGATTAAATGTGACTACAATGTCTCTCTTCATGAATTATTTTTATTTTTGAATGATGTAACCATGGTTAATATATATGTGCTTATTCGTTTCAATATTTTTTTCCTTTCCTTCAATTTGTTTTGACCCAAAGGTTCAAGTAGGAGGTTCGCCTATGTTCAAAGTGGAGAGGAAACTTGGCAAAGGTGGATTTGGACAGGTTTTTGTTGGTCGTCGAGTTACCGGTGGTAATGACCGTCTTAATGGCCCCGGTGCCACTGAGGTATGCCGAGGGTTGTAACTTGTACCTTTTATGCGGTTAAAGGATTTTCATTGGTTTTAAGTTTATTGCTATCTTTTCATATTTCAGGTGGCTTTGAAATTTGAACATAGAAACAGTAAAGGTTGCAACTACGGCCCTCCTTACGAGTGGCAAGTGTACAAGTAGGTATCTTAATTGCAGTATATTGTATAGTAAATTTTTTTAATTGCAGTATATTGTAAATTGGTTTGCATTTATGTACAAGTTTCGTGAAAAGGTAAATTTGTCTGAGATTTGTTATTTAAATTTTGTTTTGAAGCACCCTTGGTGGAAGTCATGGAATACCCAAAGTACATTATAAAGGAAGACAAGGAGAATATTACGTGATGGTATGATCTACACAATAAGCCTTGTATATCATTTCATTTCACCTTAGCCAATTAACTATTTTTTTTAATGATTATCATTATATTATATGCAGGTTATGGACATGCTTGGTCCAAGCTTGTGGGATGTTTGGAATACCACAGGCCAGGCGTGAGTATAAGCCTTTTATGATTGTATCTGCTTCTTTATTGCTCGTGTTGTACAATAATGACCGTTACATATTGATTGTATATTTTAAGATTTCTTTGTTAAGATTATATTGGAGTGAGGTGTGACTCAGTTTTAGTTTAATTTCTTCCATATGACTAGGTAACTAAATATACTAACCAAACTAAAACAAGATTAGTTATATGTTGTATAGGTGGTTTGTGGTTTGGCTATTGATATGATTTATGTAGAACATTATGGCCCCGTTTGATCCAAGTAGCGGATTCTACTTAATGGGATGAGGCTTGGTTGTTTTTCTTGTGGGTGTAAATGGTTTATACTGAACTCTTTGTGATGTCAATCTTCTGTTTTCATTTTTCGAAGAAAATTTGATCTTGACATTTGTAGAAATCTTGAATTCTACTTTATATGTAAATCACCATTCAAGTAAATTCTTTGAATATTGTAGCCTTGACCCGCTTTGTGCATTAATTATGTATTCTCCTTCACAATTGTTATATCTAAGCCTTCTATTCTGGCTGTGTCCTCTATATCTTATTTTTCCATTTTCTTCTTTGACAGTGAGCCAATAACTAATATACTCCCTTTTTTGTCTTTTCCGGTCAACATTCTGCTGTGTTTGTCTTTTTTTTTTTTTTTAATGGAGTTTATTGATTTGATTGTCTTTTGAATATGTAGGATGTCTGCCGAAATGGTGGCATGCATTGCTGTGGAGTCATTGTCGATCTTGGAGAAGATGCACTCAAGGGGGTATGTTATAGATCAAACTCATGCTTGTATTCAGCCTTTAGTTTGGTATATTGATTTAGTTTTGAGTTTTCTGGTTTCTAAATATTGCCTTTGTTCATTGTAGGTATGTGCATGGGGATGTAAAACCAGAGAACTTTTTACTAGGTCAGCCAGGTACAGCACAAGAGAAGAAATTGTTTCTTGTTGATCTCGGATTAGGTGAGTAGTTTGAATTTGAAGTGTGAGAAATTTTGTTTTTGTTTTTACCGGCCCCTTTCTTTTTATTCTTACAAGGATTTTATTTTCAGCAACAAAGTGGAGAGACACCTCCACTGGGCAGCATGTTGAATATGACCAGCGCCCTGATATGTTCAGGTTTCTTTTTTCACCTTCTTATCCTTTCTTCAAACATTTTCTTAATTTCTACTGGCATCATATTTCTTTTTCACTTTTTTTATTAGGGTAAAAATATCTAACATCTTGAGGCTTATATTTTTAATTTTTGCAGAGGAACTGTTCGATATGCAAGTGTTCATGCTCATTTGGGAAGAACTGCTAGTAGAAGAGATGATCTTGAATCTCTTGCATATACACTCATTTTTCTTCATAAAGGCCGGTTACCATGGCAAGGTTATCAGGTGCTTACACCTTTTTTATTCTCATCCAGTATTTACTGTGGCAATTGGATTCCATTACATGTTTGTTTTAATCTGTTTTTAATTTCTTTGATAGGGAGATAATAAGTCGTATCTTGTTTGCAAAAAGAAGATGGGAACATCTCCTGAGATGCTATGCTGCTTCTGCCCTACTCCTTTTAGACATTTCCTTGAGTTTGTAGTGAACATGAAATTCGATGAAGAGCCTAACTATTCCAAGCTAATATCCTTGTTTGATAGTGTGCTTGGACCAAATCCTGCACTGCGGCCAATTAATACTGAAGGTGCTCAAAAGGTAATGATGGTGGTGTTATTTTAGAGATCTTCTATTTTATTATCACGCTTTTAAACTTTTGGGTGGCGTCATTGAATTTACCATAATCATTTGTTTCCCAAATTGAAATTTACCAGGTCGGGCAAAAGAGAGGTAGATTGAATATTGAGGAAGAAGATGATTCACAGCCTAAAAAGAAGGTTCGTTTGGGGGTTCCTGCTACACAATGGATATCAATTTACAATGCAAGAATGCCAATGAAGCAAAGGTAGAGATGCAATCACTTTTTGCCCATGTTTCATTAATTTGACCAATTGGAGATATATAACTGAGCCTCATCCCATTAAGTGGATGCGGCTACATGGATCTCGAGATGCAAGAATCCTTATTATGATTTAACTTTAAAGATAAATTGTTTACCTTCGAATCTATGTTACATAAGTACGGGTACGGTACCCGGTACAATACGGGTATACAAGAGAAAGTCGCAATTTTCTTTCACAGTAGAATAATGAGCAAAAATGATGTACCGCGAGTACTGTACGTGGACCCGGTATGGGCACTTCACCTTTTTACAAGTACTCATGCTTCAGAGCTCCAAATCTATCATAATGATGTCCAAACCGCCTTAGATGCTGTTTTACTATCTTTTCTATAATGTGAGTCTAATCTAACACATGTCGTATAGATATCGGTGTCAAAGTATGCCCAACAGGCCTATATGAAGAAACCTCGGTGTCTTGCTGTGATTTACTTAGTTAATATACGGGAAAATATATATAAAACAAATCTAACCATATGATACGCCAGGGTGTGCATAGCTTGTCATTCTTTATATATAAATTTGTTTATTTCTTGTGTTTATTTATCTGACTTGGGATCTTCACAAATAAATGAGTTATGCCATGAAGTCAAAAGTATCTACTGCTTGGGTGTTTTGTTATCATGATGTTTTGGTTATACTGAAAAGTGGCTGCACTGTTAGGTATCATTACAATGTAGCGGATGGACGACTAGCGCAACATGTAGAGCGAGGGATTGCAGACGGCCTTCTTATTAGCTGTGTGGCTTCTTGTTCCAATCTCTGGGCACTTATTATGGATGCTGGAACTGGATTTACAAATCAAGTCTACAAGTTGTCACCTTTTTTCTTACACAAGGTTAAATTATATATGATGTACATCATTGCATGTGGAAAGCATTGTAACCTATTTTCAGTTGATGAAACTTACCCATCCAATTCTGCTTCTTTCTAGGAATGGATCATGGAGCAATGGGAGAAGAACTATTACATTACTTCTATAGCTGGGGTTACTAATGGGAGCTCTCTTGTGGTGATGTCAAAAGGTTTGTTTTCTCTACTTACAAATGATGAATGATATTTTTATCTGTAAATGATGATGCATTGGATCCAATTCCACGATGAATGATATTTTTATCTGTAAATGATGATGCATTGGATCCAATTCCGTGGGACTTTTTTAGTTAGGTAATTTGGGTCTGTGTTCCCTAAAAATGTTTTTTTACCATTAGTATGGTAGAGTTGTATGGTAGGAGTGTTGGGCGGTTAAGAGCCAACACGAAAATCAAGTAAGCGTAATGGAGATGAGGATGTTGTGTTGGATGATTGGTAAGACTAGACGAGATAGGACTAAGAATGACACCATTAGAGAGAGAGTTGGGGTAACACCTATAGTAGAAAAGATGGTAGAAAATAGGATTAGGTGGTTTGGGCATGTAGAGTGAAGACCGATAGATGCTGTAGTACGAACAGTAGATCGGATGGAGGAGAGTCAGATTAGAAGAGGTAGTGGAAGACCTAGAAAAACTATAAGGAAAACTATTAGGAAAGATTTAGAGGTTAATGAGTTGGATCCAAATACGGTTTCAGATAGAACATTATGGCATAATTTGATCCATGTTGCCAACCTCACTTAGTGGGATAAGGCTTGGTTGTGTTGTTGTATTAGTATATTCTAAACACCAGTAAACTATGATAAGGGCATGTGTATTAACAGATCGAATATCCCTGAAATTTGAGTGGGGGTGGGGGGTAATGATGCACCTCTTGTTGCTAATTTCATGGTTCCCCTCTACAATAAATGGGAAATTAAGAGAGAAAATAAGTATTATTATATGTTGCTTTTGAACTACCAGGTTGTCTGTAGGATCCACAGAAATGGGAGTGAGGGTGATTAGATTGTCGGTTCAATAAGCTTGGTGAATTTTATATAATTACGTTAATAAGTAGCATATTTTCATTTGGAGTAATTTGAAATTCTATTCACATGGAATTAGCTTTTTACATGATTTGTTGTGCAAGTGTGGCAAAGAGGCTCTACTCAAATTTAGTGTCTGTGTGACTTTCCATTTTTTAGCAGATCTATCTGGATTTTGTTTGTCCACATGCATGAGGGTTTCCCTTTTTTAATACTATAGAGGGAACAAATGAGAAAAAAACTTCATTGTGATTTGTTCTGGTTTCCACAGGCACACAGTATACACAACAGTCATACAAAGTAAGCGAGTCTTTCCCCTTCAAATGGATAAACAAGAAGTGGAGAGAAGGGTTTCACGTGACTTCAATGGCCACTGCTGGAAGTCGTTGGGGTGTTGTTATGTCACGGAATGCTGGATTCAGTGATCAGGTTTGCTGTTTATTTTGTATTCTAATATATTATATGATATAATGGAATAAAATCTTGAGTATTTGTTTTCCTGCCTTTATTCAAAATGTTGTGTACTTTTGTATTTCATTGAGTATTACTATTATCCTAATATATGATATGATATAATGGAATAAAATGTTGAGTATTTGTTTTCCTGCCTTTATTCAAAATGTTGTGTACTTTTGTATTTCATTGAGTATTACTATTATCACATGGCCTGGCTTTGCAAACTGGTGTGCTTGCAAAGTTGCAATCCTCTTTGGATTCTATCAGTAGTTTAGTGGTACACCCTGCCAACAAAGTTCGATAGAATTTAGCCAAAATTAACCTATTTTCTAGCTTGGTTTGTTGAAATTGGGTCTAACCCATCCCTACAAAATCAGCTTGTAAGTGCCTCATCTTTATAAACTCTTATCTAGAGTCCTATCTTTCCAAAATGGGACTTTGGGTTTTTTTTCTCAATATGGTTATTGATTTGTTGTATGGATCGTAGTAGTCGGTTCCTCTTATTGAACTCATTCTGGTTCTTGTCAAAACAATTCTTGTTTGTTGGCATTTTCCTTCACTTCACTAGTTCAAGGTTGACTTTATTAAGAAATTGCAATTGTAGGAAGTACCAATGGGTTGTTAAGCATTATAAGGTCGCTTTATACTGATCTACTTTGATATTTGGTTTGTTTTGTTGTCATCAGATTAAATTAGAGCTCACAAACCTGTCCCATAACACTAATATAACAAATTGAGTTTAAAGTGGGTCTGTGTTTTTTAAGATGCCTTGGCTTAATTTTTTTTTTTCCTTTCTGAAATTAAATAATTTGAATTTCTTGTTACTTTTAATAGAGCAACAGTTTTTTCCTTGCCTTCTCTGCACAAAGTTCGGTTGAAATTTTTTGCACTTCGATTAACGTCTCTTTTCCTTGCTTTCTCATCATCCACCTTTTATATGTAGGTCGTTGAACTTGATTTCCTCTACCCTAGCGAGGGAATCCATAGACGATGGGATAATGGTTACAGGATCACAGCAACTGCAGCTACATGGGATCAATCTGCTCTTATATTAAGCAAACCGAGGCGAAGACCTGCTGACGAAACTCAGGAAACTCTTCGGACGTCTCAATTTCCAAGCACACATGTTAAGGTTTGGGTTGATAAATCAATACTTGATTTTGTTAACTACCTTCAAAAAGTTATTTTTCTCATTGATTCTGTTTATGTTTACAGGAAAAATGGTCAAAAAACCTTTACCTTGCTTGCTTGTGCTATGGGCGTACAGTATGTTGA
Protein sequence

>MS.gene54375.t1

MGDESGGLSANKGVAQEDEGNTTPFPERVQVGGSPMFKVERKLGKGGFGQVFVGRRVTGGNDRLNGPGATEVALKFEHRNSKGCNYGPPYEWQVYNTLGGSHGIPKVHYKGRQGEYYVMVMDMLGPSLWDVWNTTGQAMSAEMVACIAVESLSILEKMHSRGYVHGDVKPENFLLGQPGTAQEKKLFLVDLGLATKWRDTSTGQHVEYDQRPDMFRGTVRYASVHAHLGRTASRRDDLESLAYTLIFLHKGRLPWQGYQGDNKSYLVCKKKMGTSPEMLCCFCPTPFRHFLEFVVNMKFDEEPNYSKLISLFDSVLGPNPALRPINTEGAQKVGQKRGRLNIEEEDDSQPKKKVRLGVPATQWISIYNARMPMKQRYHYNVADGRLAQHVERGIADGLLISCVASCSNLWALIMDAGTGFTNQVYKLSPFFLHKEWIMEQWEKNYYITSIAGVTNGSSLVVMSKGTQYTQQSYKVSESFPFKWINKKWREGFHVTSMATAGSRWGVVMSRNAGFSDQVVELDFLYPSEGIHRRWDNGYRITATAATWDQSALILSKPRRRPADETQETLRTSQFPSTHVKEKWSKNLYLACLCYGRTVC