Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54511.t1 | KYP31853.1 | 47.8 | 274 | 19 | 2 | 1 | 151 | 1042 | 1314 | 7.10E-55 | 223.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54511.t1 | P10978 | 36.9 | 268 | 46 | 1 | 2 | 146 | 1058 | 1325 | 6.5e-41 | 168.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54511.t1 | A0A151QNH6 | 47.8 | 274 | 19 | 2 | 1 | 151 | 1042 | 1314 | 5.1e-55 | 223.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene54511 | 0.825433 | 4.70E-54 | -1.69E-46 |
| MS.gene049499 | MS.gene54511 | 0.808062 | 3.72E-50 | -1.69E-46 |
| MS.gene051597 | MS.gene54511 | 0.815823 | 7.59E-52 | -1.69E-46 |
| MS.gene051699 | MS.gene54511 | 0.80888 | 2.49E-50 | -1.69E-46 |
| MS.gene052500 | MS.gene54511 | 0.801098 | 1.06E-48 | -1.69E-46 |
| MS.gene052954 | MS.gene54511 | 0.806309 | 8.76E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene54511 | 0.845057 | 5.15E-59 | -1.69E-46 |
| MS.gene054222 | MS.gene54511 | 0.824319 | 8.61E-54 | -1.69E-46 |
| MS.gene058279 | MS.gene54511 | 0.806669 | 7.35E-50 | -1.69E-46 |
| MS.gene058881 | MS.gene54511 | 0.818825 | 1.60E-52 | -1.69E-46 |
| MS.gene05952 | MS.gene54511 | 0.802734 | 4.87E-49 | -1.69E-46 |
| MS.gene060012 | MS.gene54511 | 0.800882 | 1.17E-48 | -1.69E-46 |
| MS.gene060365 | MS.gene54511 | 0.816083 | 6.64E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTATCCTTTGATTAGTTT+GGG | 0.219958 | 7.4:+91260677 | None:intergenic |
| AAACTATCCTTTGATTAGTT+TGG | 0.231148 | 7.4:+91260676 | None:intergenic |
| TGCTCATCTCTTCTTTCTCT+TGG | 0.271304 | 7.4:+91261361 | None:intergenic |
| CAAAACTCTATCTTCTCTTT+TGG | 0.321800 | 7.4:+91260727 | None:intergenic |
| TCTTTCTCTTGGTCATTCTC+AGG | 0.376250 | 7.4:+91261372 | None:intergenic |
| GCTACCCACTGCTGAAGAAT+AGG | 0.395959 | 7.4:+91261332 | None:intergenic |
| CATATCTGCTCCATTGTCAT+TGG | 0.455158 | 7.4:+91260765 | None:intergenic |
| AACACTGGAGTGCTGTGAAA+TGG | 0.463543 | 7.4:-91261224 | MS.gene54511:CDS |
| CCTGTGCATGTTCCGCTACC+TGG | 0.472224 | 7.4:-91261423 | MS.gene54511:CDS |
| CTACCCACTGCTGAAGAATA+GGG | 0.473406 | 7.4:+91261333 | None:intergenic |
| TGCAGACCATGGCGTACATA+AGG | 0.500035 | 7.4:+91261310 | None:intergenic |
| ACATCGATGTGAGGTATCAC+TGG | 0.524604 | 7.4:-91260837 | MS.gene54511:intron |
| TTGTAAAGAAGCTGCAGGCT+TGG | 0.530111 | 7.4:-91260707 | MS.gene54511:CDS |
| ATCTCAAGAAAGTTCTTGAA+AGG | 0.540106 | 7.4:-91261467 | MS.gene54511:CDS |
| CCTACAGCATAACCGATGTC+TGG | 0.542961 | 7.4:+91261282 | None:intergenic |
| CCAGACATCGGTTATGCTGT+AGG | 0.556943 | 7.4:-91261282 | MS.gene54511:CDS |
| GTCTGGCCTCGTGCAGACCA+TGG | 0.563338 | 7.4:+91261299 | None:intergenic |
| CCAGGTAGCGGAACATGCAC+AGG | 0.572979 | 7.4:+91261423 | None:intergenic |
| AAAATTCATACCAATGACAA+TGG | 0.582534 | 7.4:-91260775 | MS.gene54511:CDS |
| TTACTGAGTTTCATATGACC+AGG | 0.584762 | 7.4:+91261405 | None:intergenic |
| GTGCCCTATTCTTCAGCAGT+GGG | 0.600613 | 7.4:-91261336 | MS.gene54511:CDS |
| TCACTGGATACGTGATGTGT+TGG | 0.601006 | 7.4:-91260821 | MS.gene54511:CDS |
| GGTGCCCTATTCTTCAGCAG+TGG | 0.613159 | 7.4:-91261337 | MS.gene54511:CDS |
| ACTATCCTTTGATTAGTTTG+GGG | 0.614975 | 7.4:+91260678 | None:intergenic |
| GGGTAGCCTTATGTACGCCA+TGG | 0.620781 | 7.4:-91261316 | MS.gene54511:CDS |
| CAAACCCAGGAAAAGAACAC+TGG | 0.623987 | 7.4:-91261239 | MS.gene54511:CDS |
| AGAGAAAGAAGAGATGAGCA+AGG | 0.630245 | 7.4:-91261358 | MS.gene54511:CDS |
| GTCTGCACGAGGCCAGACAT+CGG | 0.643214 | 7.4:-91261294 | MS.gene54511:CDS |
| AGTAGATTCCTATCAAACCC+AGG | 0.660954 | 7.4:-91261252 | MS.gene54511:CDS |
| AGTTTCATATGACCAGGTAG+CGG | 0.678545 | 7.4:+91261411 | None:intergenic |
| TGTACGCCATGGTCTGCACG+AGG | 0.697121 | 7.4:-91261305 | MS.gene54511:CDS |
| GTGCTGTGAAATGGATACTG+AGG | 0.727600 | 7.4:-91261215 | MS.gene54511:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATTCATACCAATGACAA+TGG | - | chr7.4:91261407-91261426 | MS.gene54511:CDS | 25.0% |
| ! | TGCAAAAGTAATCAAATATC+CGG | + | chr7.4:91261105-91261124 | None:intergenic | 25.0% |
| !!! | ATATTTGATTACTTTTGCAG+GGG | - | chr7.4:91261106-91261125 | MS.gene54511:intron | 25.0% |
| !!! | GATATTTGATTACTTTTGCA+GGG | - | chr7.4:91261105-91261124 | MS.gene54511:intron | 25.0% |
| ATCTCAAGAAAGTTCTTGAA+AGG | - | chr7.4:91260715-91260734 | MS.gene54511:CDS | 30.0% | |
| GAACATAACTTTCTTGATTC+TGG | + | chr7.4:91261259-91261278 | None:intergenic | 30.0% | |
| ! | CAAAACTCTATCTTCTCTTT+TGG | + | chr7.4:91261458-91261477 | None:intergenic | 30.0% |
| ! | TTTTCTTGAATCTACATCTC+CGG | + | chr7.4:91261075-91261094 | None:intergenic | 30.0% |
| !! | GGATATTTGATTACTTTTGC+AGG | - | chr7.4:91261104-91261123 | MS.gene54511:intron | 30.0% |
| !! | TATTTGATTACTTTTGCAGG+GGG | - | chr7.4:91261107-91261126 | MS.gene54511:intron | 30.0% |
| AGAATTCGACATACCATTCA+AGG | - | chr7.4:91261315-91261334 | MS.gene54511:CDS | 35.0% | |
| AGTAATCAAATATCCGGATG+TGG | + | chr7.4:91261099-91261118 | None:intergenic | 35.0% | |
| GATTCAAGAAAATCCACATC+CGG | - | chr7.4:91261083-91261102 | MS.gene54511:intron | 35.0% | |
| TGGTTATGTAGATGCTGATA+TGG | - | chr7.4:91261049-91261068 | MS.gene54511:intron | 35.0% | |
| TTACTGAGTTTCATATGACC+AGG | + | chr7.4:91260780-91260799 | None:intergenic | 35.0% | |
| ! | GCCAAAACATAAGCACTTTT+TGG | + | chr7.4:91261009-91261028 | None:intergenic | 35.0% |
| ! | TGGATACTGAGGTATCTAAA+AGG | - | chr7.4:91260978-91260997 | MS.gene54511:intron | 35.0% |
| !!! | AAGTGCTTATGTTTTGGCAA+TGG | - | chr7.4:91261011-91261030 | MS.gene54511:intron | 35.0% |
| !!! | GCCAAAAAGTGCTTATGTTT+TGG | - | chr7.4:91261005-91261024 | MS.gene54511:intron | 35.0% |
| AAGAAGTTCCTGCAAGAGTT+AGG | - | chr7.4:91261233-91261252 | MS.gene54511:CDS | 40.0% | |
| AATGGAAATCAAATGCTCGC+TGG | - | chr7.4:91261029-91261048 | MS.gene54511:intron | 40.0% | |
| AGAGAAAGAAGAGATGAGCA+AGG | - | chr7.4:91260824-91260843 | MS.gene54511:CDS | 40.0% | |
| AGTAGATTCCTATCAAACCC+AGG | - | chr7.4:91260930-91260949 | MS.gene54511:intron | 40.0% | |
| AGTTTCATATGACCAGGTAG+CGG | + | chr7.4:91260774-91260793 | None:intergenic | 40.0% | |
| CATATCTGCTCCATTGTCAT+TGG | + | chr7.4:91261420-91261439 | None:intergenic | 40.0% | |
| TATGTAGATGCTGATATGGC+CGG | - | chr7.4:91261053-91261072 | MS.gene54511:intron | 40.0% | |
| TCTTTCTCTTGGTCATTCTC+AGG | + | chr7.4:91260813-91260832 | None:intergenic | 40.0% | |
| TGCTCATCTCTTCTTTCTCT+TGG | + | chr7.4:91260824-91260843 | None:intergenic | 40.0% | |
| ! | GTTCTTTTCCTGGGTTTGAT+AGG | + | chr7.4:91260941-91260960 | None:intergenic | 40.0% |
| !! | GAGTTTTGTAAAGAAGCTGC+AGG | - | chr7.4:91261470-91261489 | MS.gene54511:CDS | 40.0% |
| AAGGTTGTAAGGAGATCCTG+TGG | - | chr7.4:91261207-91261226 | MS.gene54511:intron | 45.0% | |
| CAAACCCAGGAAAAGAACAC+TGG | - | chr7.4:91260943-91260962 | MS.gene54511:intron | 45.0% | |
| CGATGTGTTTCGACCTTGAA+TGG | + | chr7.4:91261331-91261350 | None:intergenic | 45.0% | |
| CGCAATCACTGAAGGTTGTA+AGG | - | chr7.4:91261196-91261215 | MS.gene54511:intron | 45.0% | |
| CTACCCACTGCTGAAGAATA+GGG | + | chr7.4:91260852-91260871 | None:intergenic | 45.0% | |
| GAGTACATCGCAATCACTGA+AGG | - | chr7.4:91261188-91261207 | MS.gene54511:intron | 45.0% | |
| GGTATGTCGAATTCTTGCTG+AGG | + | chr7.4:91261310-91261329 | None:intergenic | 45.0% | |
| GTGCTGTGAAATGGATACTG+AGG | - | chr7.4:91260967-91260986 | MS.gene54511:intron | 45.0% | |
| TCACTGGATACGTGATGTGT+TGG | - | chr7.4:91261361-91261380 | MS.gene54511:CDS | 45.0% | |
| TGCTGAGGTGAATTGCACTT+TGG | + | chr7.4:91261295-91261314 | None:intergenic | 45.0% | |
| TTGTAAAGAAGCTGCAGGCT+TGG | - | chr7.4:91261475-91261494 | MS.gene54511:CDS | 45.0% | |
| ! | ACATCGATGTGAGGTATCAC+TGG | - | chr7.4:91261345-91261364 | MS.gene54511:CDS | 45.0% |
| ! | CACTCCAGTGTTCTTTTCCT+GGG | + | chr7.4:91260950-91260969 | None:intergenic | 45.0% |
| !! | AACACTGGAGTGCTGTGAAA+TGG | - | chr7.4:91260958-91260977 | MS.gene54511:intron | 45.0% |
| CCTACAGCATAACCGATGTC+TGG | + | chr7.4:91260903-91260922 | None:intergenic | 50.0% | |
| GATTCTGGCCTAACTCTTGC+AGG | + | chr7.4:91261244-91261263 | None:intergenic | 50.0% | |
| GCAGGAACTTCTTCATCCAC+AGG | + | chr7.4:91261226-91261245 | None:intergenic | 50.0% | |
| GCTACCCACTGCTGAAGAAT+AGG | + | chr7.4:91260853-91260872 | None:intergenic | 50.0% | |
| GGTCGAAACACATCGATGTG+AGG | - | chr7.4:91261336-91261355 | MS.gene54511:CDS | 50.0% | |
| GTGCCCTATTCTTCAGCAGT+GGG | - | chr7.4:91260846-91260865 | MS.gene54511:intron | 50.0% | |
| TGCAGACCATGGCGTACATA+AGG | + | chr7.4:91260875-91260894 | None:intergenic | 50.0% | |
| TGCGATGTACTCTGCTTCTG+TGG | + | chr7.4:91261180-91261199 | None:intergenic | 50.0% | |
| ! | CCAGACATCGGTTATGCTGT+AGG | - | chr7.4:91260900-91260919 | MS.gene54511:intron | 50.0% |
| ! | GCACTCCAGTGTTCTTTTCC+TGG | + | chr7.4:91260951-91260970 | None:intergenic | 50.0% |
| GGTGCCCTATTCTTCAGCAG+TGG | - | chr7.4:91260845-91260864 | MS.gene54511:intron | 55.0% | |
| !! | GGGTAGCCTTATGTACGCCA+TGG | - | chr7.4:91260866-91260885 | MS.gene54511:intron | 55.0% |
| CCTGTGCATGTTCCGCTACC+TGG | - | chr7.4:91260759-91260778 | MS.gene54511:CDS | 60.0% | |
| GTCTGCACGAGGCCAGACAT+CGG | - | chr7.4:91260888-91260907 | MS.gene54511:intron | 60.0% | |
| TGTACGCCATGGTCTGCACG+AGG | - | chr7.4:91260877-91260896 | MS.gene54511:intron | 60.0% | |
| TTGCAGGGGGAGCTGTCTCA+TGG | - | chr7.4:91261120-91261139 | MS.gene54511:intron | 60.0% | |
| !! | CCAGGTAGCGGAACATGCAC+AGG | + | chr7.4:91260762-91260781 | None:intergenic | 60.0% |
| GTCTGGCCTCGTGCAGACCA+TGG | + | chr7.4:91260886-91260905 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 91260690 | 91261514 | 91260690 | ID=MS.gene54511 |
| chr7.4 | mRNA | 91260690 | 91261514 | 91260690 | ID=MS.gene54511.t1;Parent=MS.gene54511 |
| chr7.4 | exon | 91261216 | 91261514 | 91261216 | ID=MS.gene54511.t1.exon1;Parent=MS.gene54511.t1 |
| chr7.4 | CDS | 91261216 | 91261514 | 91261216 | ID=cds.MS.gene54511.t1;Parent=MS.gene54511.t1 |
| chr7.4 | exon | 91260690 | 91260846 | 91260690 | ID=MS.gene54511.t1.exon2;Parent=MS.gene54511.t1 |
| chr7.4 | CDS | 91260690 | 91260846 | 91260690 | ID=cds.MS.gene54511.t1;Parent=MS.gene54511.t1 |
| Gene Sequence |
| Protein sequence |
