Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55218.t1 | AES96725.1 | 87 | 69 | 9 | 0 | 1 | 69 | 1 | 69 | 1.90E-22 | 115.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55218.t1 | G7KF79 | 87.0 | 69 | 9 | 0 | 1 | 69 | 1 | 69 | 1.4e-22 | 115.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051199 | MS.gene55218 | 0.852914 | 3.38E-61 | -1.69E-46 |
| MS.gene051664 | MS.gene55218 | 0.815273 | 1.01E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene55218 | 0.807645 | 4.57E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene55218 | 0.813377 | 2.64E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene55218 | 0.801182 | 1.02E-48 | -1.69E-46 |
| MS.gene054801 | MS.gene55218 | 0.815432 | 9.27E-52 | -1.69E-46 |
| MS.gene057444 | MS.gene55218 | 0.801879 | 7.31E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene55218 | 0.807354 | 5.27E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55218.t1 | MTR_5g040640 | 88.889 | 108 | 12 | 0 | 1 | 108 | 1 | 108 | 3.91e-66 | 194 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGCCGAACTCTTCTTTGT+CGG | 0.419224 | 5.2:+30543526 | None:intergenic |
| GGTCAGATCCTATTCACAAC+AGG | 0.431529 | 5.2:-30543450 | MS.gene55218:CDS |
| TGTTAAGCCCTGTTGTGAAT+AGG | 0.444432 | 5.2:+30543442 | None:intergenic |
| AGAAGAAGCATTATTCTAGA+AGG | 0.448569 | 5.2:+30543736 | None:intergenic |
| CTTAATATCTATTCAACCTA+AGG | 0.449957 | 5.2:-30543701 | MS.gene55218:CDS |
| GCAAAAGAAGAGTGCGAAAA+GGG | 0.462740 | 5.2:-30543471 | MS.gene55218:CDS |
| TTCAAGCCTGACACTTCCTT+AGG | 0.480333 | 5.2:+30543685 | None:intergenic |
| GTTCGGCTAATGTCAACAAC+AGG | 0.483501 | 5.2:-30543513 | MS.gene55218:CDS |
| GAATTCTTCTTTGTGTTCAA+CGG | 0.494948 | 5.2:+30543574 | None:intergenic |
| AGCAAAAGAAGAGTGCGAAA+AGG | 0.503578 | 5.2:-30543472 | MS.gene55218:CDS |
| ATTCAACCTAAGGAAGTGTC+AGG | 0.528463 | 5.2:-30543691 | MS.gene55218:CDS |
| GAAGCATTATTCTAGAAGGA+TGG | 0.538450 | 5.2:+30543740 | None:intergenic |
| TGTTCCGACAAAGAAGAGTT+CGG | 0.544409 | 5.2:-30543530 | MS.gene55218:CDS |
| TAGTCTGCAAGATACAGAGA+AGG | 0.662730 | 5.2:-30543599 | MS.gene55218:CDS |
| GTCAGATCCTATTCACAACA+GGG | 0.678647 | 5.2:-30543449 | MS.gene55218:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTTAATATCTATTCAACCTA+AGG | - | chr5.2:30543491-30543510 | MS.gene55218:CDS | 25.0% |
| !!! | ATAATGCTTCTTCTCTTTTT+TGG | - | chr5.2:30543465-30543484 | MS.gene55218:CDS | 25.0% |
| !!! | TAATGCTTCTTCTCTTTTTT+GGG | - | chr5.2:30543466-30543485 | MS.gene55218:CDS | 25.0% |
| GAATTCTTCTTTGTGTTCAA+CGG | + | chr5.2:30543621-30543640 | None:intergenic | 30.0% | |
| ! | CTTCTTTTGCTTGATTGATT+TGG | + | chr5.2:30543711-30543730 | None:intergenic | 30.0% |
| !! | AGAAGAAGCATTATTCTAGA+AGG | + | chr5.2:30543459-30543478 | None:intergenic | 30.0% |
| ! | GAAGCATTATTCTAGAAGGA+TGG | + | chr5.2:30543455-30543474 | None:intergenic | 35.0% |
| GTCAGATCCTATTCACAACA+GGG | - | chr5.2:30543743-30543762 | MS.gene55218:CDS | 40.0% | |
| TAGTCTGCAAGATACAGAGA+AGG | - | chr5.2:30543593-30543612 | MS.gene55218:CDS | 40.0% | |
| TGTTCCGACAAAGAAGAGTT+CGG | - | chr5.2:30543662-30543681 | MS.gene55218:CDS | 40.0% | |
| TTAGCCGAACTCTTCTTTGT+CGG | + | chr5.2:30543669-30543688 | None:intergenic | 40.0% | |
| ! | AGCAAAAGAAGAGTGCGAAA+AGG | - | chr5.2:30543720-30543739 | MS.gene55218:CDS | 40.0% |
| ! | GCAAAAGAAGAGTGCGAAAA+GGG | - | chr5.2:30543721-30543740 | MS.gene55218:CDS | 40.0% |
| ! | GCATTATCCTGTGCTCTTTT+TGG | + | chr5.2:30543560-30543579 | None:intergenic | 40.0% |
| !! | ATTCAACCTAAGGAAGTGTC+AGG | - | chr5.2:30543501-30543520 | MS.gene55218:CDS | 40.0% |
| GGTCAGATCCTATTCACAAC+AGG | - | chr5.2:30543742-30543761 | MS.gene55218:CDS | 45.0% | |
| GTTCGGCTAATGTCAACAAC+AGG | - | chr5.2:30543679-30543698 | MS.gene55218:CDS | 45.0% | |
| TTCAAGCCTGACACTTCCTT+AGG | + | chr5.2:30543510-30543529 | None:intergenic | 45.0% | |
| ! | GACATAGCCAAAAAGAGCAC+AGG | - | chr5.2:30543550-30543569 | MS.gene55218:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 30543444 | 30543770 | 30543444 | ID=MS.gene55218 |
| chr5.2 | mRNA | 30543444 | 30543770 | 30543444 | ID=MS.gene55218.t1;Parent=MS.gene55218 |
| chr5.2 | exon | 30543444 | 30543770 | 30543444 | ID=MS.gene55218.t1.exon1;Parent=MS.gene55218.t1 |
| chr5.2 | CDS | 30543444 | 30543770 | 30543444 | ID=cds.MS.gene55218.t1;Parent=MS.gene55218.t1 |
| Gene Sequence |
| Protein sequence |
