Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55278.t1 | XP_024626105.1 | 84.1 | 189 | 30 | 0 | 1 | 189 | 1 | 189 | 4.40E-70 | 274.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55278.t1 | A0A396HFV7 | 63.2 | 193 | 67 | 2 | 1 | 189 | 1 | 193 | 2.7e-45 | 191.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051932 | MS.gene55278 | 0.892561 | 1.42E-74 | -1.69E-46 |
| MS.gene053491 | MS.gene55278 | 0.943959 | 4.68E-103 | -1.69E-46 |
| MS.gene06044 | MS.gene55278 | 0.905459 | 4.20E-80 | -1.69E-46 |
| MS.gene061245 | MS.gene55278 | 0.884186 | 2.38E-71 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55278.t1 | MTR_8g007240 | 33.333 | 174 | 66 | 7 | 17 | 188 | 49 | 174 | 6.28e-16 | 72.0 |
| MS.gene55278.t1 | MTR_0903s0010 | 30.055 | 183 | 72 | 6 | 14 | 187 | 42 | 177 | 3.33e-15 | 70.5 |
| MS.gene55278.t1 | MTR_3g102970 | 31.953 | 169 | 65 | 7 | 17 | 183 | 49 | 169 | 1.90e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGAAATTGGAATTGAATT+TGG | 0.155038 | 5.2:+31601097 | MS.gene55278:CDS |
| AAAATCCAAAGAGGAAATTT+TGG | 0.165439 | 5.2:+31600729 | MS.gene55278:CDS |
| TCCGATTACTTAAAGCAATT+TGG | 0.183536 | 5.2:+31601061 | MS.gene55278:CDS |
| CATCTCCAAAATTTCCTCTT+TGG | 0.214318 | 5.2:-31600734 | None:intergenic |
| ATTGAATTTGGAAAAGAATT+AGG | 0.225866 | 5.2:+31601109 | MS.gene55278:CDS |
| TTTGGAGATGTCGAAACTTT+TGG | 0.226089 | 5.2:+31600747 | MS.gene55278:CDS |
| ACCATTGTAATTTGTTCATA+TGG | 0.234426 | 5.2:+31600861 | MS.gene55278:CDS |
| GCCAAATTGCTTTAAGTAAT+CGG | 0.251128 | 5.2:-31601062 | None:intergenic |
| TTTGGTAAATTTCCTCCAAT+TGG | 0.356380 | 5.2:-31600659 | None:intergenic |
| AAAGAGATTGGGAAGGAAAT+TGG | 0.360942 | 5.2:+31601085 | MS.gene55278:CDS |
| AAGCAATTTGGCAAAGAGAT+TGG | 0.365860 | 5.2:+31601073 | MS.gene55278:CDS |
| CCATTGTAATTTGTTCATAT+GGG | 0.394671 | 5.2:+31600862 | MS.gene55278:CDS |
| CCCAACTACAGCCTTCTTTG+AGG | 0.420158 | 5.2:-31601017 | None:intergenic |
| AGCAATTTGGCAAAGAGATT+GGG | 0.422113 | 5.2:+31601074 | MS.gene55278:CDS |
| TGTTTGCACTTGTGTTGTCA+TGG | 0.425538 | 5.2:+31601221 | MS.gene55278:CDS |
| TCAGCTGTTGGGTATCCAAT+TGG | 0.433222 | 5.2:+31600644 | MS.gene55278:CDS |
| TTAAGCATTGCTCTAACTCT+AGG | 0.453670 | 5.2:-31600902 | None:intergenic |
| GGTAGCAAAGCTTCAGCTGT+TGG | 0.480338 | 5.2:+31600632 | MS.gene55278:CDS |
| GTAGCAAAGCTTCAGCTGTT+GGG | 0.484076 | 5.2:+31600633 | MS.gene55278:CDS |
| TATTTGCGTGAAAGTGTCTC+TGG | 0.485310 | 5.2:+31601139 | MS.gene55278:CDS |
| TGAGGACACATCATTATTTG+AGG | 0.495657 | 5.2:-31600999 | None:intergenic |
| GTTTGCACTTGTGTTGTCAT+GGG | 0.499878 | 5.2:+31601222 | MS.gene55278:CDS |
| TCCTCAAAGAAGGCTGTAGT+TGG | 0.512316 | 5.2:+31601016 | MS.gene55278:CDS |
| TAATGATGTGTCCTCAAAGA+AGG | 0.517374 | 5.2:+31601006 | MS.gene55278:CDS |
| TTCCAGTACCTTTCAAAATG+TGG | 0.526356 | 5.2:+31600976 | MS.gene55278:CDS |
| GTAATCAGATGATGAAATTG+TGG | 0.528968 | 5.2:+31600690 | MS.gene55278:CDS |
| ATCTGATTACAACCACACTT+TGG | 0.542708 | 5.2:-31600677 | None:intergenic |
| GCTGTTGGGTATCCAATTGG+AGG | 0.550598 | 5.2:+31600647 | MS.gene55278:CDS |
| CCTCAAAGAAGGCTGTAGTT+GGG | 0.553172 | 5.2:+31601017 | MS.gene55278:CDS |
| TAATCAGATGATGAAATTGT+GGG | 0.555715 | 5.2:+31600691 | MS.gene55278:CDS |
| GGAGGAAATTTACCAAAGTG+TGG | 0.573900 | 5.2:+31600665 | MS.gene55278:CDS |
| ATTTGGAAAAGAATTAGGCA+AGG | 0.603871 | 5.2:+31601114 | MS.gene55278:CDS |
| ACAACACAAGTGCAAACAGT+AGG | 0.614937 | 5.2:-31601216 | None:intergenic |
| CCCATATGAACAAATTACAA+TGG | 0.636074 | 5.2:-31600862 | None:intergenic |
| ATTGCTGCAAAGAACAGTGA+AGG | 0.636453 | 5.2:+31600929 | MS.gene55278:CDS |
| ATACAGATGAAAATCCAAAG+AGG | 0.650224 | 5.2:+31600720 | MS.gene55278:CDS |
| ATTTGGCAAAGAGATTGGGA+AGG | 0.653755 | 5.2:+31601078 | MS.gene55278:CDS |
| AAGAACAGTGAAGGTGATGG+TGG | 0.677445 | 5.2:+31600938 | MS.gene55278:CDS |
| GCAAAGAACAGTGAAGGTGA+TGG | 0.689878 | 5.2:+31600935 | MS.gene55278:CDS |
| ATTAGGGTTCCTGAAATGGG+GGG | 0.693811 | 5.2:+31600611 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGAATTTGGAAAAGAATT+AGG | + | chr5.2:31601109-31601128 | MS.gene55278:CDS | 20.0% |
| !!! | AAATCTGATTTGTATGTATT+TGG | + | chr5.2:31600785-31600804 | MS.gene55278:intron | 20.0% |
| ! | AAGGAAATTGGAATTGAATT+TGG | + | chr5.2:31601097-31601116 | MS.gene55278:CDS | 25.0% |
| ! | TAATCAGATGATGAAATTGT+GGG | + | chr5.2:31600691-31600710 | MS.gene55278:CDS | 25.0% |
| !! | AAAATCCAAAGAGGAAATTT+TGG | + | chr5.2:31600729-31600748 | MS.gene55278:CDS | 25.0% |
| !!! | ACCATTGTAATTTGTTCATA+TGG | + | chr5.2:31600861-31600880 | MS.gene55278:CDS | 25.0% |
| !!! | CCATTGTAATTTGTTCATAT+GGG | + | chr5.2:31600862-31600881 | MS.gene55278:CDS | 25.0% |
| ATACAGATGAAAATCCAAAG+AGG | + | chr5.2:31600720-31600739 | MS.gene55278:CDS | 30.0% | |
| ATTTGGAAAAGAATTAGGCA+AGG | + | chr5.2:31601114-31601133 | MS.gene55278:CDS | 30.0% | |
| CCCATATGAACAAATTACAA+TGG | - | chr5.2:31600865-31600884 | None:intergenic | 30.0% | |
| GTAATCAGATGATGAAATTG+TGG | + | chr5.2:31600690-31600709 | MS.gene55278:CDS | 30.0% | |
| TCCGATTACTTAAAGCAATT+TGG | + | chr5.2:31601061-31601080 | MS.gene55278:CDS | 30.0% | |
| TTTGGTAAATTTCCTCCAAT+TGG | - | chr5.2:31600662-31600681 | None:intergenic | 30.0% | |
| ! | GCCAAATTGCTTTAAGTAAT+CGG | - | chr5.2:31601065-31601084 | None:intergenic | 30.0% |
| AAAGAGATTGGGAAGGAAAT+TGG | + | chr5.2:31601085-31601104 | MS.gene55278:CDS | 35.0% | |
| AAGCAATTTGGCAAAGAGAT+TGG | + | chr5.2:31601073-31601092 | MS.gene55278:CDS | 35.0% | |
| AGCAATTTGGCAAAGAGATT+GGG | + | chr5.2:31601074-31601093 | MS.gene55278:CDS | 35.0% | |
| ATCTGATTACAACCACACTT+TGG | - | chr5.2:31600680-31600699 | None:intergenic | 35.0% | |
| CATCTCCAAAATTTCCTCTT+TGG | - | chr5.2:31600737-31600756 | None:intergenic | 35.0% | |
| TAATGATGTGTCCTCAAAGA+AGG | + | chr5.2:31601006-31601025 | MS.gene55278:CDS | 35.0% | |
| TGAGGACACATCATTATTTG+AGG | - | chr5.2:31601002-31601021 | None:intergenic | 35.0% | |
| TTCCAGTACCTTTCAAAATG+TGG | + | chr5.2:31600976-31600995 | MS.gene55278:CDS | 35.0% | |
| ! | TTAAGCATTGCTCTAACTCT+AGG | - | chr5.2:31600905-31600924 | None:intergenic | 35.0% |
| ! | TTGGAGATGTCGAAACTTTT+GGG | + | chr5.2:31600748-31600767 | MS.gene55278:CDS | 35.0% |
| ! | TTTGGAGATGTCGAAACTTT+TGG | + | chr5.2:31600747-31600766 | MS.gene55278:CDS | 35.0% |
| !! | ATTTGAGGCCACATTTTGAA+AGG | - | chr5.2:31600987-31601006 | None:intergenic | 35.0% |
| ACAACACAAGTGCAAACAGT+AGG | - | chr5.2:31601219-31601238 | None:intergenic | 40.0% | |
| ATTGCTGCAAAGAACAGTGA+AGG | + | chr5.2:31600929-31600948 | MS.gene55278:CDS | 40.0% | |
| ATTTGGCAAAGAGATTGGGA+AGG | + | chr5.2:31601078-31601097 | MS.gene55278:CDS | 40.0% | |
| GGAGGAAATTTACCAAAGTG+TGG | + | chr5.2:31600665-31600684 | MS.gene55278:CDS | 40.0% | |
| GTTTGCACTTGTGTTGTCAT+GGG | + | chr5.2:31601222-31601241 | MS.gene55278:CDS | 40.0% | |
| TGTTTGCACTTGTGTTGTCA+TGG | + | chr5.2:31601221-31601240 | MS.gene55278:CDS | 40.0% | |
| !! | TATTTGCGTGAAAGTGTCTC+TGG | + | chr5.2:31601139-31601158 | MS.gene55278:CDS | 40.0% |
| !!! | GATGGTGGTTTTAGCAACAT+TGG | + | chr5.2:31600953-31600972 | MS.gene55278:CDS | 40.0% |
| CCTCAAAGAAGGCTGTAGTT+GGG | + | chr5.2:31601017-31601036 | MS.gene55278:CDS | 45.0% | |
| GCAAAGAACAGTGAAGGTGA+TGG | + | chr5.2:31600935-31600954 | MS.gene55278:CDS | 45.0% | |
| GTAGCAAAGCTTCAGCTGTT+GGG | + | chr5.2:31600633-31600652 | MS.gene55278:CDS | 45.0% | |
| TCAGCTGTTGGGTATCCAAT+TGG | + | chr5.2:31600644-31600663 | MS.gene55278:CDS | 45.0% | |
| TCCTCAAAGAAGGCTGTAGT+TGG | + | chr5.2:31601016-31601035 | MS.gene55278:CDS | 45.0% | |
| ! | AAGAACAGTGAAGGTGATGG+TGG | + | chr5.2:31600938-31600957 | MS.gene55278:CDS | 45.0% |
| ! | GAGATGTCGAAACTTTTGGG+AGG | + | chr5.2:31600751-31600770 | MS.gene55278:CDS | 45.0% |
| !! | GGCCACATTTTGAAAGGTAC+TGG | - | chr5.2:31600981-31601000 | None:intergenic | 45.0% |
| CCCAACTACAGCCTTCTTTG+AGG | - | chr5.2:31601020-31601039 | None:intergenic | 50.0% | |
| GCTGTTGGGTATCCAATTGG+AGG | + | chr5.2:31600647-31600666 | MS.gene55278:CDS | 50.0% | |
| GGTAGCAAAGCTTCAGCTGT+TGG | + | chr5.2:31600632-31600651 | MS.gene55278:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 31600626 | 31601276 | 31600626 | ID=MS.gene55278 |
| chr5.2 | mRNA | 31600626 | 31601276 | 31600626 | ID=MS.gene55278.t1;Parent=MS.gene55278 |
| chr5.2 | exon | 31600626 | 31600772 | 31600626 | ID=MS.gene55278.t1.exon1;Parent=MS.gene55278.t1 |
| chr5.2 | CDS | 31600626 | 31600772 | 31600626 | ID=cds.MS.gene55278.t1;Parent=MS.gene55278.t1 |
| chr5.2 | exon | 31600854 | 31601276 | 31600854 | ID=MS.gene55278.t1.exon2;Parent=MS.gene55278.t1 |
| chr5.2 | CDS | 31600854 | 31601276 | 31600854 | ID=cds.MS.gene55278.t1;Parent=MS.gene55278.t1 |
| Gene Sequence |
| Protein sequence |
