AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55984

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55984.t1 MTR_2g033910 84.146 410 12 2 1 410 1 357 0.0 690
MS.gene55984.t1 MTR_7g081020 50.356 421 137 6 6 414 1 361 9.20e-145 419
MS.gene55984.t1 MTR_1g112840 51.190 420 141 5 1 412 1 364 1.04e-141 412
MS.gene55984.t1 MTR_8g074930 50.952 420 140 6 1 412 1 362 2.38e-137 400
MS.gene55984.t1 MTR_1g067210 48.655 409 148 4 8 408 1 355 7.86e-127 373
MS.gene55984.t1 MTR_0015s0140 44.526 411 169 5 1 407 1 356 6.34e-124 366
MS.gene55984.t1 MTR_7g100240 48.889 405 140 5 12 408 5 350 2.00e-122 362
MS.gene55984.t1 MTR_8g074930 63.563 247 90 0 166 412 4 250 2.71e-113 335
MS.gene55984.t1 MTR_7g081020 47.934 363 117 6 6 356 1 303 2.47e-112 334
MS.gene55984.t1 MTR_1g106855 46.296 378 147 4 30 405 16 339 1.11e-110 332
MS.gene55984.t1 MTR_1g116260 60.236 254 100 1 151 404 103 355 4.63e-108 325
MS.gene55984.t1 MTR_1g116260 53.684 95 32 3 4 90 5 95 1.30e-26 110
MS.gene55984.t1 MTR_0015s0140 57.085 247 105 1 161 407 57 302 4.46e-102 308
MS.gene55984.t1 MTR_1g106855 42.470 332 135 4 30 359 16 293 2.36e-81 254
MS.gene55984.t1 MTR_3g032660 43.774 265 138 4 149 404 85 347 6.30e-69 226
MS.gene55984.t1 MTR_3g032700 43.774 265 138 4 149 404 85 347 6.93e-69 226
MS.gene55984.t1 MTR_3g032590 44.882 254 133 3 156 404 102 353 1.02e-68 226
MS.gene55984.t1 MTR_7g090550 44.403 268 140 4 151 411 93 358 2.58e-66 219
MS.gene55984.t1 MTR_7g090530 44.656 262 136 4 156 411 97 355 3.41e-63 211
MS.gene55984.t1 MTR_4g113480 30.933 375 194 7 34 404 34 347 2.48e-51 182
MS.gene55984.t1 MTR_4g113345 28.535 396 192 13 12 404 3 310 2.44e-41 150
MS.gene55984.t1 MTR_7g090540 42.786 201 92 5 215 410 5 187 5.82e-38 139
MS.gene55984.t1 MTR_7g060770 26.426 333 192 6 75 403 263 546 3.23e-35 138
MS.gene55984.t1 MTR_4g119830 26.835 395 249 10 22 403 120 487 4.08e-32 128
MS.gene55984.t1 MTR_4g076560 31.381 239 152 4 167 401 250 480 1.13e-30 124
MS.gene55984.t1 MTR_4g098650 29.268 287 144 5 171 403 486 767 5.94e-30 124
MS.gene55984.t1 MTR_4g063905 27.636 275 157 5 166 403 426 695 4.61e-28 118
MS.gene55984.t1 MTR_1g085530 34.483 174 105 3 234 403 715 883 1.34e-22 101
MS.gene55984.t1 MTR_4g113210 32.704 159 91 6 190 337 104 257 1.57e-17 82.4
MS.gene55984.t1 MTR_3g464650 29.524 210 101 6 197 405 153 316 3.83e-15 76.6
MS.gene55984.t1 MTR_3g464700 32.026 153 73 4 197 349 177 298 1.05e-14 75.5
MS.gene55984.t1 MTR_7g093240 31.757 148 65 4 210 355 6 119 1.42e-14 70.9
MS.gene55984.t1 MTR_7g025640 28.704 216 115 8 192 403 6 186 1.57e-13 69.7
MS.gene55984.t1 MTR_5g071550 29.487 156 79 4 197 352 213 337 1.64e-13 72.4
MS.gene55984.t1 MTR_2g040500 25.874 286 141 10 72 352 264 483 4.36e-13 71.6
MS.gene55984.t1 MTR_5g024340 22.552 337 144 9 75 406 124 348 1.20e-12 69.7
MS.gene55984.t1 MTR_3g105730 27.751 209 112 6 164 371 77 247 1.55e-12 68.2
MS.gene55984.t1 MTR_3g105730 27.751 209 112 6 164 371 99 269 1.57e-12 68.6
MS.gene55984.t1 MTR_3g105880 28.324 173 90 4 195 363 158 300 2.34e-12 68.2
MS.gene55984.t1 MTR_3g105880 28.571 161 81 4 195 351 158 288 3.14e-12 68.2
MS.gene55984.t1 MTR_1g115570 27.451 204 105 7 164 366 160 321 3.57e-12 67.8
MS.gene55984.t1 MTR_4g007440 27.673 159 81 4 197 351 160 288 5.09e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55984.t1 AT3G17090 61.337 419 99 4 1 410 1 365 1.75e-175 497
MS.gene55984.t1 AT3G12620 49.880 415 144 5 4 410 6 364 1.04e-137 401
MS.gene55984.t1 AT3G12620 49.880 415 144 5 4 410 6 364 1.04e-137 401
MS.gene55984.t1 AT4G38520 50.239 418 144 5 1 410 1 362 1.30e-137 401
MS.gene55984.t1 AT4G38520 50.239 418 144 5 1 410 1 362 1.30e-137 401
MS.gene55984.t1 AT5G66080 48.463 423 153 5 1 414 1 367 2.93e-136 397
MS.gene55984.t1 AT3G17090 56.712 365 95 4 1 356 1 311 7.80e-136 394
MS.gene55984.t1 AT3G51370 47.743 421 154 5 1 413 1 363 1.68e-135 395
MS.gene55984.t1 AT3G51370 47.743 421 154 5 1 413 1 363 1.68e-135 395
MS.gene55984.t1 AT3G55050 48.130 401 162 4 4 404 5 359 1.29e-126 373
MS.gene55984.t1 AT3G55050 48.130 401 162 4 4 404 5 359 1.29e-126 373
MS.gene55984.t1 AT5G66080 61.044 249 97 0 166 414 15 263 1.97e-112 333
MS.gene55984.t1 AT5G06750 44.681 376 151 4 36 408 43 364 5.10e-110 331
MS.gene55984.t1 AT5G06750 44.681 376 151 4 36 408 43 364 5.10e-110 331
MS.gene55984.t1 AT5G02760 47.607 397 156 8 8 404 1 345 1.34e-109 329
MS.gene55984.t1 AT3G51370 58.065 248 104 0 166 413 31 278 2.37e-108 323
MS.gene55984.t1 AT4G33920 45.407 381 149 5 30 406 16 341 3.54e-108 325
MS.gene55984.t1 AT4G33920 54.800 250 108 2 161 406 10 258 6.94e-94 286
MS.gene55984.t1 AT5G06750 52.157 255 119 1 157 408 28 282 1.67e-93 285
MS.gene55984.t1 AT4G33920 56.332 229 95 2 182 406 1 228 2.44e-88 271
MS.gene55984.t1 AT3G16560 28.818 347 212 9 65 406 167 483 4.80e-37 142
MS.gene55984.t1 AT3G16560 28.818 347 212 9 65 406 167 483 4.80e-37 142
MS.gene55984.t1 AT3G16560 28.818 347 212 9 65 406 167 483 4.80e-37 142
MS.gene55984.t1 AT5G02400 25.253 396 222 8 75 403 277 665 7.45e-33 132
MS.gene55984.t1 AT2G28890 25.459 381 222 8 75 403 275 645 1.48e-31 128
MS.gene55984.t1 AT3G09400 24.548 387 218 9 75 403 271 641 2.08e-31 127
MS.gene55984.t1 AT1G07630 25.594 379 226 8 75 403 281 653 3.35e-31 127
MS.gene55984.t1 AT3G16560 28.662 314 189 9 65 373 167 450 5.14e-30 122
MS.gene55984.t1 AT2G35350 26.190 294 166 5 166 413 492 780 1.53e-26 113
MS.gene55984.t1 AT2G46920 25.850 294 152 5 171 403 547 835 2.23e-26 113
MS.gene55984.t1 AT2G46920 25.850 294 152 5 171 403 547 835 2.23e-26 113
MS.gene55984.t1 AT2G46920 25.850 294 152 5 171 403 547 835 2.23e-26 113
MS.gene55984.t1 AT2G46920 25.850 294 152 5 171 403 547 835 2.23e-26 113
MS.gene55984.t1 AT3G09400 23.249 357 200 9 75 373 271 611 1.97e-23 103
MS.gene55984.t1 AT2G40180 24.706 340 152 10 68 407 154 389 2.38e-16 81.3
MS.gene55984.t1 AT1G07160 24.672 381 172 13 25 405 113 378 3.61e-16 80.5
MS.gene55984.t1 AT2G30020 27.751 209 106 6 197 405 231 394 1.74e-14 75.5
MS.gene55984.t1 AT3G16800 30.093 216 102 9 199 403 76 253 1.76e-14 73.6
MS.gene55984.t1 AT3G16800 30.093 216 102 9 199 403 76 253 1.76e-14 73.6
MS.gene55984.t1 AT3G16800 30.093 216 102 9 199 403 171 348 1.90e-14 75.1
MS.gene55984.t1 AT3G16800 30.093 216 102 9 199 403 171 348 1.90e-14 75.1
MS.gene55984.t1 AT3G16800 30.093 216 102 9 199 403 171 348 1.90e-14 75.1
MS.gene55984.t1 AT4G31750 29.048 210 108 7 164 371 98 268 1.99e-14 74.3
MS.gene55984.t1 AT4G31750 29.048 210 108 7 164 371 98 268 1.99e-14 74.3
MS.gene55984.t1 AT4G31750 29.048 210 108 7 164 371 98 268 2.09e-14 74.3
MS.gene55984.t1 AT1G67820 26.339 224 118 6 166 383 154 336 1.71e-13 72.4
MS.gene55984.t1 AT1G67820 26.339 224 118 6 166 383 190 372 2.50e-13 72.0
MS.gene55984.t1 AT4G31860 28.395 162 82 4 195 352 158 289 3.78e-13 70.5
MS.gene55984.t1 AT4G31860 28.395 162 82 4 195 352 158 289 4.24e-13 70.9
MS.gene55984.t1 AT2G25070 24.490 294 160 10 74 356 51 293 7.82e-13 70.1
MS.gene55984.t1 AT2G25070 24.490 294 160 10 74 356 51 293 7.82e-13 70.1
MS.gene55984.t1 AT3G51470 28.095 210 110 6 164 368 96 269 2.97e-12 67.8
MS.gene55984.t1 AT5G10740 27.619 210 111 7 164 371 98 268 3.11e-12 68.2
MS.gene55984.t1 AT5G10740 27.619 210 111 7 164 371 98 268 3.11e-12 68.2
MS.gene55984.t1 AT4G26080 25.322 233 131 7 179 403 225 422 3.40e-12 68.6
MS.gene55984.t1 AT4G28400 27.830 212 110 9 164 372 79 250 3.67e-12 67.0
MS.gene55984.t1 AT4G28400 27.830 212 110 9 164 372 101 272 4.66e-12 67.0
MS.gene55984.t1 AT3G51470 28.095 210 110 6 164 368 140 313 4.70e-12 67.8
MS.gene55984.t1 AT2G20630 28.708 209 106 10 164 369 97 265 4.81e-12 67.0
MS.gene55984.t1 AT2G20630 28.708 209 106 10 164 369 97 265 6.24e-12 66.6
MS.gene55984.t1 AT5G24940 27.536 207 109 7 164 368 98 265 7.48e-12 67.4
MS.gene55984.t1 AT2G33700 27.803 223 104 7 195 411 181 352 8.28e-12 67.0
MS.gene55984.t1 AT1G43900 28.283 198 103 6 164 361 188 346 1.38e-11 66.2

Find 125 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTATGGTTACTGAATTA+TGG 0.158490 2.3:+56735887 MS.gene55984:CDS
TATTTGCATCAGATGGTTTA+TGG 0.193260 2.3:+56739064 MS.gene55984:CDS
AGTCTGAACTTCGCATTAAT+TGG 0.255336 2.3:-56738958 None:intergenic
CGACTGCTTGGTCATTATTC+AGG 0.280326 2.3:-56739092 None:intergenic
TTTCGTGATATCGGAAAATA+TGG 0.294437 2.3:+56734364 MS.gene55984:CDS
GTGCAGGTTTCTAAATCTAT+TGG 0.301600 2.3:+56738898 MS.gene55984:intron
GGGCCGCTTGGAACGTTTGT+AGG 0.306358 2.3:+56734457 MS.gene55984:CDS
CATGGAGTGTGGAGAGTAAA+AGG 0.310288 2.3:+56736147 MS.gene55984:CDS
TTGCTTTGGTTTCGTGATAT+CGG 0.311697 2.3:+56734355 MS.gene55984:CDS
CTCCATGTTGGAAGCCGTTC+GGG 0.314964 2.3:+56734278 MS.gene55984:CDS
AACCATCTGATGCAAATATA+AGG 0.317523 2.3:-56739059 None:intergenic
GATCAGTGCCAGATCGAATC+TGG 0.325566 2.3:+56734436 MS.gene55984:CDS
GAACTGTACCTCTAGTTATA+AGG 0.332040 2.3:-56740689 None:intergenic
AAGGGTCCTGCCGGAGGGTT+GGG 0.332701 2.3:+56742648 MS.gene55984:CDS
TGAAACATGCACAGTTTAAC+CGG 0.338094 2.3:+56738932 MS.gene55984:CDS
ATTTGCATCAGATGGTTTAT+GGG 0.358280 2.3:+56739065 MS.gene55984:CDS
TCTCCATGTTGGAAGCCGTT+CGG 0.377884 2.3:+56734277 MS.gene55984:CDS
ACTTCGCATTAATTGGTTCC+CGG 0.388120 2.3:-56738951 None:intergenic
ATAGGCATGTGCATTGGTTC+AGG 0.390801 2.3:-56738982 None:intergenic
GAGAGTAAAAGGCATTATTC+AGG 0.403956 2.3:+56736158 MS.gene55984:CDS
CTGCTTAGCGCTTTGGGTGG+TGG 0.409260 2.3:-56735333 None:intergenic
CGGCCTCAAGTTGCAACTAC+TGG 0.412317 2.3:+56735916 MS.gene55984:CDS
CAAGGGTCCTGCCGGAGGGT+TGG 0.413339 2.3:+56742647 MS.gene55984:CDS
GGAAAGAAAGTTGGGAATAC+TGG 0.418008 2.3:+56736015 MS.gene55984:CDS
AATATAAGGAATGAATCATT+TGG 0.419645 2.3:-56739045 None:intergenic
AGAGGATGACAAAGAATAGA+TGG 0.423381 2.3:-56739018 None:intergenic
TGGCTCCGGTGAATTCTCTA+TGG 0.426824 2.3:+56734384 MS.gene55984:CDS
ACTTATCAAGGCGGCCCTGC+AGG 0.433634 2.3:+56740554 MS.gene55984:CDS
TGACAGCAGTGTTGCTGTGT+TGG 0.435804 2.3:-56735301 None:intergenic
AACTACTGGAACATGTTGTT+TGG 0.437834 2.3:+56735930 MS.gene55984:CDS
CTTGTCTATTTGAAGCTTCG+TGG 0.439831 2.3:+56742709 MS.gene55984:CDS
CTCTGTTTGCAGTAGACAGA+TGG 0.445361 2.3:-56735385 None:intergenic
TGGGAATACTGGTGGGGTTG+CGG 0.445784 2.3:+56736026 MS.gene55984:CDS
AAGGGAAAGACGGATTGCTT+TGG 0.448079 2.3:+56734341 MS.gene55984:CDS
TTCCTTATATTTGCATCAGA+TGG 0.450656 2.3:+56739057 MS.gene55984:CDS
GGAGATTCCCGTGTTGTGTT+GGG 0.456061 2.3:+56735994 MS.gene55984:CDS
CGTGGTTGCAGTCGCTGGGT+TGG 0.460605 2.3:+56742493 MS.gene55984:intron
CACAGTTTCAAGGGTCCTGC+CGG 0.460779 2.3:+56742639 MS.gene55984:CDS
GATGGATTAGCAGACAAGAT+AGG 0.466083 2.3:-56739000 None:intergenic
AGCGGCATCAAGTGAGTCAC+GGG 0.468668 2.3:+56735807 MS.gene55984:intron
AGGGTTGGGATTCGTTTAGA+TGG 0.471315 2.3:+56742662 MS.gene55984:CDS
GATTCTTCATCCGCCGTCGT+TGG 0.475591 2.3:+56734313 MS.gene55984:CDS
GGCGGTGCAGCACAGTTTCA+AGG 0.476261 2.3:+56742629 MS.gene55984:intron
TGTTGGTTTGCTGCTAGCGG+TGG 0.477402 2.3:-56735284 None:intergenic
GTAGGTGTTTATGATGGTCA+TGG 0.477486 2.3:+56734475 MS.gene55984:CDS
GTAGCTGTTGTGCTGCTGTT+CGG 0.485397 2.3:-56735410 None:intergenic
GCATTTCGTTTAACAGAAGA+GGG 0.489261 2.3:+56735859 MS.gene55984:CDS
AGAAGAGGGGTTTACTGCTA+TGG 0.494563 2.3:+56735873 MS.gene55984:CDS
TGTGGACAATCTCGACTGCT+TGG 0.496220 2.3:-56739104 None:intergenic
GGCCGCCTTGATAAGTCTCT+TGG 0.497413 2.3:-56740547 None:intergenic
TCCGCCGTCGTTGGAAGAGA+AGG 0.497429 2.3:+56734322 MS.gene55984:CDS
TAAGGAATGAATCATTTGGT+TGG 0.504243 2.3:-56739041 None:intergenic
CCAAAGCGCTAAGCAGCAAA+AGG 0.505712 2.3:+56735340 MS.gene55984:CDS
GGTTCGTGGTTGCAGTCGCT+GGG 0.506614 2.3:+56742489 MS.gene55984:intron
GTGGTGGTTTGCGCTCGCTT+TGG 0.506699 2.3:+56742731 MS.gene55984:CDS
TGCGGCTATTCAGCTGTCTA+CGG 0.508719 2.3:+56736044 MS.gene55984:CDS
CAAGCGGCCCAGATTCGATC+TGG 0.509802 2.3:-56734444 None:intergenic
GTTGGTTTGCTGCTAGCGGT+GGG 0.510999 2.3:-56735283 None:intergenic
AGGAGATTCCCGTGTTGTGT+TGG 0.513663 2.3:+56735993 MS.gene55984:CDS
TGCTTGAGGACAACAATTTG+AGG 0.514445 2.3:-56736126 None:intergenic
AGTTTCAAGGGTCCTGCCGG+AGG 0.516248 2.3:+56742642 MS.gene55984:CDS
TGTTGGAAGCCGTTCGGGCA+CGG 0.517969 2.3:+56734283 MS.gene55984:CDS
TTCGTTTAGATGGTCGGAGG+TGG 0.518075 2.3:+56742672 MS.gene55984:CDS
CAAACGTAACGAGCAGCATC+AGG 0.523423 2.3:-56734502 None:intergenic
TATTTGAAGCTTCGTGGTGG+TGG 0.526911 2.3:+56742715 MS.gene55984:CDS
TTGGGATTCGTTTAGATGGT+CGG 0.527175 2.3:+56742666 MS.gene55984:CDS
GGCATTTCGTTTAACAGAAG+AGG 0.528426 2.3:+56735858 MS.gene55984:CDS
CAAATTGTTGTCCTCAAGCA+TGG 0.531700 2.3:+56736129 MS.gene55984:CDS
CTTTCTTTCCCAACACAACA+CGG 0.535912 2.3:-56736002 None:intergenic
TTGTGTTGGGAAAGAAAGTT+GGG 0.536092 2.3:+56736007 MS.gene55984:CDS
TGAATCTTCTCTCTCCATGT+TGG 0.537709 2.3:+56734266 MS.gene55984:CDS
GTTGTGTTGGGAAAGAAAGT+TGG 0.537924 2.3:+56736006 MS.gene55984:CDS
TGTTACGTGATGGCAGTAGC+AGG 0.540618 2.3:-56735438 None:intergenic
CCCTTCTCTTCCAACGACGG+CGG 0.542100 2.3:-56734323 None:intergenic
ACGAATCCCAACCCTCCGGC+AGG 0.548770 2.3:-56742654 None:intergenic
ACTGGAACATGTTGTTTGGT+TGG 0.551780 2.3:+56735934 MS.gene55984:CDS
CTTTCTTGTCAATTGAACGA+AGG 0.554021 2.3:-56740611 None:intergenic
AATGAATCATTTGGTTGGAG+AGG 0.557534 2.3:-56739036 None:intergenic
CAGATCGAATCTGGGCCGCT+TGG 0.557847 2.3:+56734445 MS.gene55984:CDS
CTGTGTTGGTTTGCTGCTAG+CGG 0.561303 2.3:-56735287 None:intergenic
TGCCCGAACGGCTTCCAACA+TGG 0.562732 2.3:-56734280 None:intergenic
ATCAGTGCCAGATCGAATCT+GGG 0.564429 2.3:+56734437 MS.gene55984:CDS
CAGCGGCATCAAGTGAGTCA+CGG 0.566808 2.3:+56735806 MS.gene55984:intron
AGAAAGTTGGGAATACTGGT+GGG 0.576053 2.3:+56736019 MS.gene55984:CDS
CTCTCCACACTCCATGCTTG+AGG 0.578750 2.3:-56736140 None:intergenic
ACGTTTGTAGGTGTTTATGA+TGG 0.580505 2.3:+56734469 MS.gene55984:CDS
TCGTCACCACCGTGCCCGAA+CGG 0.580862 2.3:-56734292 None:intergenic
GACAAGATAGGCATGTGCAT+TGG 0.581356 2.3:-56738988 None:intergenic
TTTCTTTCCCAACACAACAC+GGG 0.583499 2.3:-56736001 None:intergenic
GTTTCAAGGGTCCTGCCGGA+GGG 0.583904 2.3:+56742643 MS.gene55984:CDS
TGAGTCACGGGGTGTTGTGA+CGG 0.586691 2.3:+56735819 MS.gene55984:CDS
TTACAGCACGTGGATTTCTG+TGG 0.590906 2.3:-56739122 None:intergenic
TAGTGCCAAGAGACTTATCA+AGG 0.592072 2.3:+56740542 MS.gene55984:CDS
GATATCGGAAAATATGGCTC+CGG 0.593745 2.3:+56734370 MS.gene55984:CDS
GTTCCAGTAGTTGCAACTTG+AGG 0.596211 2.3:-56735919 None:intergenic
TTTCCCTTCTCTTCCAACGA+CGG 0.598111 2.3:-56734326 None:intergenic
GCGGTGCAGCACAGTTTCAA+GGG 0.598540 2.3:+56742630 MS.gene55984:intron
CCGCCGTCGTTGGAAGAGAA+GGG 0.602549 2.3:+56734323 MS.gene55984:CDS
GTCTATTTGAAGCTTCGTGG+TGG 0.605589 2.3:+56742712 MS.gene55984:CDS
ACAACAATTTGAGGATCATG+AGG 0.613663 2.3:-56736117 None:intergenic
AAGAAAGTTGGGAATACTGG+TGG 0.616734 2.3:+56736018 MS.gene55984:CDS
GGAACATAATGCGAATCTTG+AGG 0.616990 2.3:+56736065 MS.gene55984:CDS
CTAAACGAATCCCAACCCTC+CGG 0.623451 2.3:-56742658 None:intergenic
TTACTGAATTATGGAATACT+CGG 0.623526 2.3:+56735896 MS.gene55984:CDS
GAAAGTTGGGAATACTGGTG+GGG 0.628709 2.3:+56736020 MS.gene55984:CDS
GGATTCGTTTAGATGGTCGG+AGG 0.629243 2.3:+56742669 MS.gene55984:CDS
AACAGCCATAGAGAATTCAC+CGG 0.629641 2.3:-56734389 None:intergenic
AATCATGACCTTATAACTAG+AGG 0.635665 2.3:+56740681 MS.gene55984:CDS
GACGGAAGAGACAATTCGAT+CGG 0.636247 2.3:+56735837 MS.gene55984:CDS
CACGAAGCTTCAAATAGACA+AGG 0.638087 2.3:-56742708 None:intergenic
GTTGGAAGAGAAGGGAAAGA+CGG 0.642524 2.3:+56734331 MS.gene55984:CDS
GAAACATGCACAGTTTAACC+GGG 0.656840 2.3:+56738933 MS.gene55984:CDS
GTAAAGAATCTTACAGCACG+TGG 0.659821 2.3:-56739132 None:intergenic
CAACAATTTGAGGATCATGA+GGG 0.663544 2.3:-56736116 None:intergenic
CAATTGACAAGAAAGTTCGA+CGG 0.666447 2.3:+56740619 MS.gene55984:CDS
TTGCTGCTTAGCGCTTTGGG+TGG 0.667958 2.3:-56735336 None:intergenic
CATTTCGTTTAACAGAAGAG+GGG 0.676787 2.3:+56735860 MS.gene55984:CDS
TTGTCCTCAAGCATGGAGTG+TGG 0.679153 2.3:+56736136 MS.gene55984:CDS
ACACCTACAAACGTTCCAAG+CGG 0.681057 2.3:-56734460 None:intergenic
GGTGTTTATGATGGTCATGG+TGG 0.681253 2.3:+56734478 MS.gene55984:CDS
GTCTGACACATGTTACGTGA+TGG 0.701439 2.3:-56735448 None:intergenic
TTGGTTTGCTGCTAGCGGTG+GGG 0.707876 2.3:-56735282 None:intergenic
TGGAAGCCGTTCGGGCACGG+TGG 0.717560 2.3:+56734286 MS.gene55984:CDS
GCGGCATCAAGTGAGTCACG+GGG 0.742751 2.3:+56735808 MS.gene55984:intron
TGCCAAGAGACTTATCAAGG+CGG 0.760577 2.3:+56740545 MS.gene55984:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.3 gene 56734250 56742756 56734250 ID=MS.gene55984
chr2.3 mRNA 56734250 56742756 56734250 ID=MS.gene55984.t1;Parent=MS.gene55984
chr2.3 exon 56734250 56734519 56734250 ID=MS.gene55984.t1.exon1;Parent=MS.gene55984.t1
chr2.3 CDS 56734250 56734519 56734250 ID=cds.MS.gene55984.t1;Parent=MS.gene55984.t1
chr2.3 exon 56735272 56735461 56735272 ID=MS.gene55984.t1.exon2;Parent=MS.gene55984.t1
chr2.3 CDS 56735272 56735461 56735272 ID=cds.MS.gene55984.t1;Parent=MS.gene55984.t1
chr2.3 exon 56735809 56736179 56735809 ID=MS.gene55984.t1.exon3;Parent=MS.gene55984.t1
chr2.3 CDS 56735809 56736179 56735809 ID=cds.MS.gene55984.t1;Parent=MS.gene55984.t1
chr2.3 exon 56738904 56739140 56738904 ID=MS.gene55984.t1.exon4;Parent=MS.gene55984.t1
chr2.3 CDS 56738904 56739140 56738904 ID=cds.MS.gene55984.t1;Parent=MS.gene55984.t1
chr2.3 exon 56740540 56740702 56740540 ID=MS.gene55984.t1.exon5;Parent=MS.gene55984.t1
chr2.3 CDS 56740540 56740702 56740540 ID=cds.MS.gene55984.t1;Parent=MS.gene55984.t1
chr2.3 exon 56742504 56742540 56742504 ID=MS.gene55984.t1.exon6;Parent=MS.gene55984.t1
chr2.3 CDS 56742504 56742540 56742504 ID=cds.MS.gene55984.t1;Parent=MS.gene55984.t1
chr2.3 exon 56742639 56742756 56742639 ID=MS.gene55984.t1.exon7;Parent=MS.gene55984.t1
chr2.3 CDS 56742639 56742756 56742639 ID=cds.MS.gene55984.t1;Parent=MS.gene55984.t1
Gene Sequence

>MS.gene55984

ATGCTTCAGTCATTGATGAATCTTCTCTCTCCATGTTGGAAGCCGTTCGGGCACGGTGGTGACGATTCTTCATCCGCCGTCGTTGGAAGAGAAGGGAAAGACGGATTGCTTTGGTTTCGTGATATCGGAAAATATGGCTCCGGTGAATTCTCTATGGCTGTTGTTCAAGCGAATCAGATTCTTGAAGATCAGTGCCAGATCGAATCTGGGCCGCTTGGAACGTTTGTAGGTGTTTATGATGGTCATGGTGGACCTGATGCTGCTCGTTACGTTTGTGATCATTTGTTTCGTAATTTTCAAGGTTTTTGTGATTTTCTCTTATGTAGGGTTAGTTCAATTCGATTCTTTTTCGTGTTTGTTATATGTGATGATGATTTTCACGGGTTTGATTTCTCGTGATCGTTTTTAATTTTTTTTTTTTTTTTTTGCTTTTTCACGTGAATCTAATAATCAGTAGTAGTTGCAAATTTCACGTGGATACTGTCTTTGATAAGTTTGATCCATGAATAATAGACACGGGATACCAGACACTTATAATAATTTGAGAAAATGACATAATTCAATGTAATTAAAGTGTTCGTGTCGGTCCTTCCTTTGGTCGTGGGTTGGAATCCAGCCCTAAGAATTAGCAATGTTAAATCTTTGCCGCTCATTGTGGCCCTATCCGGCATGAGGGATTGGTTGTGCGCAGAGATACATGGTTCATACCAAAAAAGTGTCGGTGTCGTGTCAGCGTCAGACATTTTTTTTTTTTTTTTTTTTGAAACAGCAAAATATTATTACCAACAAGCCCGTGCGAGATGCACTAATAAGCTCGGGTGGAAAAAAAACATACAAGGGGAAATCGGTTGCCACAAAACACCGGCTCGAACAAAAACCCCTTAAAAACCACCCCACAAGAAAAGAACTCACAACTGGTCGGAACTAGGCAACCAGAAGCTACACAAAAACAGCAGTAGCAGTATCGCTTCTAACCCAACTCCCCCTCCAGACATACACACTAACAACAACCACACCTACAGCACAAAAACTCCCCACCGCTAGCAGCAAACCAACACAGCAACACTGCTGTCAAGACAGCAGCCACCACCCAAAGCGCTAAGCAGCAAAAGGACGCCAACCGCAGCCAAAAAAACCATCTGTCTACTGCAAACAGAGCACCGAACAGCAGCACAACAGCTACTAAAACCTGCTACTGCCATCACGTAACATGTGTCAGACACAGACACAGGATACACCTTTGATCAGAATTGTGTCAGTGCAGAGAGTTTAATTGCGTATGAGTTTTTCTCGTCGTTTTTGTAGGTTTGAATTGAATGTAGTAAAAGTGTTGTTGATTTTTTTATATGTTGATTAAAATGGCTCTTGTTGTCTATTAAACAATGACATGCTGACACCAGCAATAATTTGTAAAAATGGCATAAATTGAATGTAATCACAGGTCATGTTGATGTCGTGTTGGTGTTTGACACTGACATGTGTCAGACACATGTTTAATGAGAAGTGTATGTGCTAGAGAGCTTGTTGTTTCTGTGTTGAACTATCTTTTGTTTGTGCAGCGGCATCAAGTGAGTCACGGGGTGTTGTGACGGAAGAGACAATTCGATCGGCATTTCGTTTAACAGAAGAGGGGTTTACTGCTATGGTTACTGAATTATGGAATACTCGGCCTCAAGTTGCAACTACTGGAACATGTTGTTTGGTTGGAGCGATTTTTCAGCAGACTCTTTTTATTGCAAATCTAGGAGATTCCCGTGTTGTGTTGGGAAAGAAAGTTGGGAATACTGGTGGGGTTGCGGCTATTCAGCTGTCTACGGAACATAATGCGAATCTTGAGGCTGTTAGACATGAACTTAAAGAATTACACCCTCATGATCCTCAAATTGTTGTCCTCAAGCATGGAGTGTGGAGAGTAAAAGGCATTATTCAGGTACATTTTTTATTATTGAATCATTGAATGTGGTTGACTATTTGACGTTTATTGATTAATGATTGCTTTAGTTTTTCTCTGTGTTTTCTCCTTGAAGTGTTGTACATTTATTTTGAGTTTTGATAATTGGATAATAGGCTGGTGTGTGTTCCTCGTGCTATCTATTCCCACCCTTACTATTTTTTGCAATGTTGTTGCCAAAAATTCCCCCAAAGTACCTAAGTAAATAAGGAGTTGTTTCATAGAGATTTATAGTGAGTGATGATTGGTTATAATGTTCAAAGTTGAATTAAACAATCCCATGTAGTGTATCAGGCTAAACTGTTTGCCTTTCAATTGCCGCATACCATTGCTATAAATATTATTGAGTGTCGTCCATATGCAAGTTTAAAAATGACAAGTACATACATTCACAAAAATAAAACATAAAATGATAAAATTCATTTGCTTGGTTTAGACTTCAGAGCTGTCTATGTGCCTCTCAAAATCTGATAATTTTTGTACTCTGGGTTAAATGCACAGAATATGATACATCAGTGTACACAGCAATGCAGAAAAATTTAACATTTGTATTTATACTTTTATTTATTTATTCATTTTGTCGAGTCTGAAAAATGAAACCATCCCCCTCCTTGAATTCCATGCTTCATATTATCGTATGTTTAGTGTAGTATATTCCATATTGAAATATCAAAAACAGTTGTAGTCTCAATTTGAGTGGAAGAACTGAACATGTCAATATCATAGTTGTAGTCTCGAATTATTAATGGTATCTATTACTTGTCTCCTATGTGTTAGTGTTTTTATCTGGTGTCCTACGTGTCAGTGTTGTATTAGGTGACCACATGGTAGTTTTTTTGTCGGGTGTCCTATGTGTCGGTGCTTCATCAAACTTGTCTCCTACGTGTCAGTGTTGTATTATGCTGTAATGAATTCTAGAAAAATATCGCACTGAGAAATAGATAGAGTGGTTTAGGTAGGGGTGATTGAGGATAAGAAAAAATATTGATGATTGAAGAATCATAATGTATGTGGTTCAGAGGAAAGGGAAGGAATTTAGGTTACAGGCTAAATTTAACATCAGTGTACACAGCAATGCAGAAAAATTTAACATTTGTATTTATACTTTTATTTATTTATTCATTTTGTCGAGTCTGAAAAATGAAACCATCCCCCTCCTTGAATTCCATGCTTCATATTATCGTATGTTTAGTGTAGTATATTCCATATTGAAATATCAAAAACAGTTGTAGTCTCAATTTGAGTGGAAGAACTGAACATGTCAATATCATAGTTGTAGTCTCGAATTATTAATGGTATCTATTACTTGTCTCCTATGTGTTAGTGTTTTTATCTGGTGTCCTACGTGTCAGTGTTGTATTAGGTGACCACATGGTAGTTTTTTTATGTGTCGGTGCTTCATCAAACTTGTCGGGTGTCCTATGTGTCGGTGCTTCATCAAACTTGTCTCCTACGTGTCAGTGTTGTATTATGCTGTAATGAATTCTAGAAAAATATCGCACTGAGAAATAGATAGAGTGGTTTAGGTAGGGGTGATTGAGGATAAGAAAAAATATTGATGATTGAAGAATCATAATGTATGTGGTTCAGAGGAAAGGGAAGGAATTTAGGTTACAGGCTAAATTGGTGCACATTTCACGGGAAAGAAATATGCTATCATACCTCAAAATTATAATATCGTGAGACTGCTTGCAGCAATGGGTGTACAAGGAAGTTTCATTTGATTGATTGAAACAGGGATTATTATGCTGTAATGAATTCTAGAAAAATATCGCACTGAGAAATAGATAGAGTGGTTTAGGTAGGGGTGATTGAGGATAAGAAAAAATATTGATGATTGAAGAATCATAATGTATGTGGTTCAGAGGAAAGGGAAGGAATTTAGGTTACAGGCTAAATTGGTGCACATTTCACGGGAAAGAAATATGCTATCATACCTCAAAATTATAATATCATGAGACTGCTTGCAGCAATGGGTGTTTCATAGCAGCACACTACAAGCAAGTTTCATTTGATTGATTGAAACAGCGATTATTATGTGGCCTATAATTTGCTATGGGCGGCAGCTGTCAACTCTAATAATAATTTCCAAGTTTCCAAAGGATATAAAGTTATGAAAGACGATGATGTTTCATAGATTCTTAGAATTTGGGTTGGGTTTAACTCATCCTTGCAAAACTGGCTTGTAAGGTGAGGACTACCCGTCACTTATAAACACATTGTCTGACCACATCTCATCCAATTTGAAACTCTTAAGACACTCCCAACGTCGAGGATGTCCAGGACTGGACGATTGGAGCGTGGACTCATGACATTTTTTATTTGAAAAATTTAAATAATTTTATCATGGATGTCATGAGATAAGAAATAGAAACAGGAAATCCGTTTTTACTGTGCTTTCTATTTTGTTTATCAGTACACTGGTAAAACAGTGTTTCAAGTATGGCTTTTGTGAGAAATAGATGGATTTATTTGCATTATTGTCTCGGTTACGTATAAGAAAAACAAATGTGGTGTCAATCACATCCAGTTTTTAAATATGCTTGTCCACTTTTAACACTGGCTTTTTTTTACTTAATTTATGAAAATGTTTTCAGTTTTCCCAAGTACCTCTTGTAACCATTATCTTATTTGTTAGTGTTGCTTTCCACACACTGATTTAATGAAAATATCACATCACTACTTCCATGATATTTGGTGCAGGTTTCTAAATCTATTGGAGACGTATATATGAAACATGCACAGTTTAACCGGGAACCAATTAATGCGAAGTTCAGACTTCCTGAACCAATGCACATGCCTATCTTGTCTGCTAATCCATCTATTCTTTGTCATCCTCTCCAACCAAATGATTCATTCCTTATATTTGCATCAGATGGTTTATGGGAGCACCTGAATAATGACCAAGCAGTCGAGATTGTCCACAGAAATCCACGTGCTGTAAGATTCTTTACTCTTTATTTTTCTTACTGCACTTATTTGCATACTCTCTGTACGTAGTTTCCTTATTCTATGGGTGTAAGTGCGAGTTTGACAGCACTAGTGTGTGTAATTGCATCTTTACAAGTCATACTATTCACTATCCATAATTTCTCAACTGCTTGTCTTGTGCTTTCAAGCCTTGTTATCGGTATCTTAGAAATTAGAATGCATGTGTGTCACACCTTGATTTGATACAAAACTAAACCTAATCGATGTTAATCTTTAGCGTGTACCTATTGTGGACATGAATCCCATCTGGAATCCTGATTCATTCTTATGATCGACTACCTAAACTCAGTGTAACTAACCACTTGATTTTGTCAACTTTGTATAGCCGGTCACTTTTCGTTGTCATTTGATTTACAACATGAATCTTGAATTATTGAGGTTCATATTTGATATACTTGTAATTTTCCTAGGTCACTTGTATTCATTTTGCTACGAGACAGAGTAGGATGGGAATGCTTGTTTCTAATTCAGCCTTAAAATTTGGTCAGGTTAAATCTTGTGTAAACTAAGACGTGTCCTTGTAGTACGAGAATGAGTTAATCCAAAGTACAGGATAATATAACAATGTCTAAAATACTCATACGATGGGTAAATATAGGTAGTTTCTGCCTTGATTATAATATTAGGATATTGTTAGGAAATTAACAATAACATACCACTTTACATGGAAAATATATTTTCAGATAAATAAATGAAAGTATGGAACAGATGAAAACCAAGTCGGACATGGAGTGAAACCCGAAGTTAGACTATAAAACAGGACTCTATTAAACCTATTTTTGTTATGAGACATAATCAAAGCTGTTCTTAAATGGCTCACTTCTGTTGGGAATCCAAGAGACTGGAATTCTTTAACAGAAGAAGGAAAGTACGGAATATTCTCAAAGGAGCGCTTTCCTTCACACTGGATTACCCAGTCATAGAACTCGATCATTTGAAGCTATCTCTTCCCCTCTACCATTGAGGGTTATTTAAAGACCGTCTAAGAATAACCAAAATAATAACAGAACCTAACAAACTAATTAACTTTTCTAGCACTCTTAACTACTATAATTATCCTTTATTCTATAATCCTAACAACCTTATACCAGATTGGATCTTTCCATTAGTATGGGATGTGTTCCTCTAATATTTGAGCAGCTTATTTAGGTTGAAATATAATTTCATGATATGGCTTTTGCATTAAAGTTATCCGCAAATATGGAGTCAATCATGTTGAGGGGTGATTTTTAGCTGGTTGTTGATTTTGTTGTTTCTCTCACAGCCTTGCTGCCATTCTCTTTGCTATATTTATTGCATAGCTTTGCTTATATTAATTGTGTTGATCTATGTATACATGTTAATGCAGGGTAGTGCCAAGAGACTTATCAAGGCGGCCCTGCAGGAAGCAGCCAGAAAACGTGAAATGCGATATTCAGACCTTCGTTCAATTGACAAGAAAGTTCGACGGCATTTTCATGACGATATAACTGTTATTGTTTTATTCTTAAATCATGACCTTATAACTAGAGGTACAGTTCAAAACCCGCCACTGTCAATTCGAAGTGCTCTCAATCACTGACTTGTATGATTGTAAGCATGATTTGTCTTTTCCGGGTATTCTGGCAACGGTATCAATACTTAGAAGATACACACACCACACCACTGGTACGTTGCCAGAATGCACATACAGCAAACCCTTGCTAACAGTGGTGCCGGCAAGTATGTTACTGATATTGAGATATAACTGGGTCAACTTATTGGTTAAACTCTTGATTTATAACACGTTAGAAATTTTATGTGCATGAAAACGACCAAAAAAGAATCAGAAAATTAACTATTGGTTTAGTAATTCCCCTATTTTGACATAATGTTGTTACCCAAAATGAACTAATTGAGAATATCTATTCTCTATATATGAAACTTAACGTCTACCAAAAATACAATCTTACTCCTTCATTTTTTGACGTTATCAAAATTGTATCATGTTGTGTCTTTTGCTTCAATCTCCCCCTATTTTTTCTCCCTTAAAAACAAATTAAAACAAACTCTCTCGATAAATCAGAAAACAACACTAACTTAATATTTTTTAAATAACAAACAAACTTAATCTTAATCTCAATAAAAACAAATTCTCCTATAATTCAACTTCTAAATATCTAAAGAGTTAAAAACAATTTACAATCTCTTTAAAAAAAACAATTTACAATCTATTATCTTATTATCTAATTATGAATGTTAATGACATGTAAAAATACAAGTTTGTCCTATGTTAATATTTTGATACATCAACATATATTTACCCTTTTGTATCTTTTGTAACAAAAGATCCAATTTAGTTGAGAGAGAGAGAGAGTAACTTTCAAAAGATTGATCAACCCTAAAAAAAAAAAAAACTCACTAATCACGGGTGCAACCCAGTGAACTCATATATGAGCGCGAAACCAAGTTACGAAAAATGAAAAGTGAACATATGTGTAGAATAACTAATGGATTGACCGTCATCACGATATCAAAATTCAAGTTTTAAGAACTAACTAAATAAACAACAAGCATATTTGAATTAACCCGTGCGGAGCACGGACCCGTGCTTAGCACGGGCCAGTGCTTAACACGGGTTTGTTGCTAGTTAATTAAATAGTATTGAAATCAAAGCAATCATAAAACTTAAAATATATTTTTATGTTAAGATATCACTGGCTCTCTTAAATCAGATTTGTTGCAAAACCGTTTGTAGCAATCAAATGTAGACTAGTATTTCTGTAATTTGTTGGGGAATCGATTGAAGCATAACACACACGCAGCCATTTACTCGCTCACCATATCATTCAAGCACAGCAATGATACTCCCCTCCGATACTCTTTATAAAATATATTGTTCAACAAAAGTTGTTGCATTTGATCCATATATGAATCAAATAAATCCATTTTTATTGATTATGGGCCCGTTTGTTACGGATTAAAATAAAAGTGAATTTTACATAAAATAATTTAGAGATTATATTTTAGAGATTTTATGAAAAATTAGAATCTATTGTGTTTGTTTACCGTAACAAAAATTAGAATCTCTCTACCCTTTCCTTTCTCTACCTTCTCTCTAAACAAACCACTCCACCGCCTTACACTTTCCCCTCCGATCCAAATCAAAACCAGCAAGATGTAGACCTTACACGCGGTGGTGCGACGAGATCCCTTCTCCGCACCACCGTTCCACCCCCTTCTCTTTGTCTGAACCCTTTCCATCCCTGTCTTCATCCTTTTCCTTGGTTTTCGTCGTCGTTCGCCGCCGTTCACAAGGTAGTGGTTTGATCCGGTTTTGGTTCGTGGTTGCAGTCGCTGGGTTGGTTCGTTTGCTGTTCGCTGAGACCTCGTAGGTGGTGGTGCGCCGCTGCTGTTTCTGTTATCCTCCTATCTGTTTGGTTCCTTGGTTGACGGTGTGTTGTTGTGCTCTCGTTCATGGCGGTGCAGCACAGTTTCAAGGGTCCTGCCGGAGGGTTGGGATTCGTTTAGATGGTCGGAGGTGGTGTCGCTGTGCTACCTTGTCTATTTGAAGCTTCGTGGTGGTGGTTTGCGCTCGCTTTGGTGA
Protein sequence

>MS.gene55984.t1

MLQSLMNLLSPCWKPFGHGGDDSSSAVVGREGKDGLLWFRDIGKYGSGEFSMAVVQANQILEDQCQIESGPLGTFVGVYDGHGGPDAARYHKNSPPLAANQHSNTAVKTAATTQSAKQQKDANRSQKNHLSTANRAPNSSTTATKTCYCHHVTSASSESRGVVTEETIRSAFRLTEEGFTAMVTELWNTRPQVATTGTCCLVGAIFQQTLFIANLGDSRVVLGKKVGNTGGVAAIQLSTEHNANLEAVRHELKELHPHDPQIVVLKHGVWRVKGIIQVSKSIGDVYMKHAQFNREPINAKFRLPEPMHMPILSANPSILCHPLQPNDSFLIFASDGLWEHLNNDQAVEIVHRNPRAGSAKRLIKAALQEAARKREMRYSDLRSIDKKVRRHFHDDITVIVLFLNHDLITRVAGLVRLLFAETSTVSRVLPEGWDSFRWSEVVSLCYLVYLKLRGGGLRSLW