AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74229

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74229.t1 MTR_1g116260 99.736 379 1 0 1 379 1 379 0.0 785
MS.gene74229.t1 MTR_1g112840 68.033 366 109 2 13 370 12 377 0.0 510
MS.gene74229.t1 MTR_7g081020 66.938 369 114 2 10 370 4 372 1.64e-180 506
MS.gene74229.t1 MTR_8g074930 65.684 373 122 1 4 370 3 375 3.59e-179 503
MS.gene74229.t1 MTR_7g100240 68.023 344 109 1 13 356 5 347 6.61e-170 479
MS.gene74229.t1 MTR_1g067210 61.725 371 133 2 9 370 1 371 1.09e-166 471
MS.gene74229.t1 MTR_2g033910 57.584 356 138 4 5 355 4 351 5.65e-151 431
MS.gene74229.t1 MTR_7g081020 67.333 300 96 1 10 307 4 303 3.77e-145 414
MS.gene74229.t1 MTR_0015s0140 53.619 373 152 5 8 368 7 370 8.71e-140 403
MS.gene74229.t1 MTR_1g106855 58.735 332 127 2 28 357 17 340 1.64e-137 397
MS.gene74229.t1 MTR_8g074930 69.582 263 74 1 114 370 1 263 3.92e-129 372
MS.gene74229.t1 MTR_0015s0140 56.391 266 111 2 110 371 55 319 1.77e-104 311
MS.gene74229.t1 MTR_1g106855 55.516 281 115 2 28 306 17 289 2.63e-104 310
MS.gene74229.t1 MTR_3g032660 45.536 336 167 6 42 370 37 363 2.94e-101 306
MS.gene74229.t1 MTR_3g032700 44.940 336 169 6 42 370 37 363 2.25e-100 304
MS.gene74229.t1 MTR_7g090530 46.462 325 157 6 38 355 34 348 5.14e-95 291
MS.gene74229.t1 MTR_7g090550 45.706 326 160 6 38 355 35 351 7.46e-91 280
MS.gene74229.t1 MTR_3g032590 45.055 273 141 5 105 370 99 369 1.11e-78 249
MS.gene74229.t1 MTR_4g113480 37.994 329 185 5 31 355 34 347 9.61e-66 218
MS.gene74229.t1 MTR_4g113345 34.043 329 176 10 34 358 22 313 2.90e-49 169
MS.gene74229.t1 MTR_7g060770 30.508 295 176 6 80 355 263 547 4.42e-43 157
MS.gene74229.t1 MTR_4g076560 32.302 291 175 6 79 355 201 483 6.93e-43 156
MS.gene74229.t1 MTR_4g119830 27.964 329 178 6 80 354 164 487 1.43e-39 147
MS.gene74229.t1 MTR_7g090540 43.229 192 86 5 168 354 7 180 4.63e-39 140
MS.gene74229.t1 MTR_4g063905 31.228 285 154 5 108 355 417 696 7.44e-36 139
MS.gene74229.t1 MTR_1g085530 28.859 298 147 5 118 355 592 884 3.46e-34 134
MS.gene74229.t1 MTR_4g098650 29.352 293 148 5 117 355 481 768 6.97e-34 133
MS.gene74229.t1 MTR_4g113210 32.353 272 140 10 31 288 16 257 2.66e-31 119
MS.gene74229.t1 MTR_3g464650 28.472 288 148 8 72 357 86 317 1.42e-28 114
MS.gene74229.t1 MTR_5g071550 31.119 286 139 10 71 354 145 374 9.35e-28 112
MS.gene74229.t1 MTR_3g464700 30.435 230 118 5 72 300 110 298 3.17e-27 110
MS.gene74229.t1 MTR_1g115570 29.010 293 136 10 69 357 116 340 3.20e-25 105
MS.gene74229.t1 MTR_1g014640 30.690 290 135 10 72 358 101 327 1.55e-23 100
MS.gene74229.t1 MTR_1g013400 30.690 290 135 10 72 358 101 327 1.55e-23 100
MS.gene74229.t1 MTR_3g105730 30.662 287 133 11 69 352 55 278 2.25e-23 99.4
MS.gene74229.t1 MTR_3g105730 30.662 287 133 11 69 352 33 256 2.38e-23 98.6
MS.gene74229.t1 MTR_4g094208 30.496 282 144 11 47 323 32 266 1.01e-21 94.0
MS.gene74229.t1 MTR_3g091060 29.487 234 119 7 70 300 116 306 1.20e-21 95.5
MS.gene74229.t1 MTR_5g071550 28.879 232 123 6 71 301 145 335 1.31e-21 95.1
MS.gene74229.t1 MTR_5g063940 28.710 310 150 12 47 352 37 279 2.10e-20 90.5
MS.gene74229.t1 MTR_5g063940 29.474 285 133 11 72 352 12 232 2.20e-20 89.4
MS.gene74229.t1 MTR_2g078760 28.276 290 134 9 72 354 31 253 3.72e-20 89.4
MS.gene74229.t1 MTR_7g093240 36.000 150 60 4 159 306 4 119 4.93e-20 85.5
MS.gene74229.t1 MTR_2g033000 29.452 292 131 10 79 354 99 331 5.90e-20 90.5
MS.gene74229.t1 MTR_2g078760 28.276 290 134 9 72 354 110 332 6.10e-20 90.1
MS.gene74229.t1 MTR_5g024340 27.273 319 149 12 80 379 124 378 8.46e-20 90.1
MS.gene74229.t1 MTR_2g008850 29.021 286 129 12 74 352 14 232 1.15e-19 87.4
MS.gene74229.t1 MTR_7g025640 29.767 215 112 7 144 354 7 186 1.50e-19 86.3
MS.gene74229.t1 MTR_3g104710 29.352 293 150 10 80 354 286 539 2.02e-19 90.1
MS.gene74229.t1 MTR_3g074610 27.070 314 150 11 47 352 37 279 2.63e-19 87.4
MS.gene74229.t1 MTR_4g125810 29.583 240 115 7 76 302 249 447 4.48e-19 89.0
MS.gene74229.t1 MTR_4g116420 26.871 294 141 11 79 359 123 355 6.13e-19 87.8
MS.gene74229.t1 MTR_2g008850 28.772 285 131 11 74 352 60 278 6.36e-19 86.3
MS.gene74229.t1 MTR_5g080680 28.448 232 127 5 80 304 132 331 1.78e-18 86.7
MS.gene74229.t1 MTR_1g014190 26.007 273 118 6 79 302 51 288 8.26e-18 84.3
MS.gene74229.t1 MTR_1g015110 29.392 296 146 12 80 354 290 543 1.81e-17 84.3
MS.gene74229.t1 MTR_4g094542 29.268 246 119 10 80 317 150 348 1.92e-17 83.2
MS.gene74229.t1 MTR_1g014190 26.374 273 117 7 79 302 89 326 2.18e-17 83.2
MS.gene74229.t1 MTR_3g068200 25.773 291 162 9 80 354 123 375 2.74e-17 83.2
MS.gene74229.t1 MTR_3g107880 26.481 287 149 12 79 359 62 292 1.88e-16 79.7
MS.gene74229.t1 MTR_1g050520 27.698 278 143 10 82 354 420 644 2.12e-16 81.3
MS.gene74229.t1 MTR_3g107880 26.481 287 149 12 79 359 120 350 2.16e-16 80.1
MS.gene74229.t1 MTR_3g107880 26.481 287 149 12 79 359 119 349 2.29e-16 80.1
MS.gene74229.t1 MTR_3g105880 26.866 268 122 8 79 302 51 288 2.58e-16 79.3
MS.gene74229.t1 MTR_3g105880 26.119 268 124 8 79 302 51 288 2.60e-16 79.7
MS.gene74229.t1 MTR_8g463130 26.644 289 152 11 39 318 1 238 2.70e-16 78.2
MS.gene74229.t1 MTR_8g463130 26.644 289 152 11 39 318 29 266 3.57e-16 78.6
MS.gene74229.t1 MTR_4g123080 28.782 271 132 9 64 318 86 311 4.63e-16 79.3
MS.gene74229.t1 MTR_1g086350 26.230 305 155 11 66 356 103 351 4.93e-16 79.3
MS.gene74229.t1 MTR_1g086350 26.230 305 155 11 66 356 87 335 4.99e-16 79.3
MS.gene74229.t1 MTR_4g007440 26.866 268 122 8 79 302 51 288 5.02e-16 79.0
MS.gene74229.t1 MTR_4g123080 28.094 299 152 10 36 318 78 329 5.13e-16 79.3
MS.gene74229.t1 MTR_1g028300 26.266 316 173 10 48 354 115 379 5.69e-16 79.0
MS.gene74229.t1 MTR_5g009370 29.258 229 124 6 80 304 87 281 5.95e-16 78.6
MS.gene74229.t1 MTR_2g040500 27.823 248 122 7 80 302 267 482 9.53e-16 79.0
MS.gene74229.t1 MTR_7g112490 26.498 317 153 14 80 359 91 364 5.93e-15 75.9
MS.gene74229.t1 MTR_7g112490 26.498 317 153 14 80 359 109 382 1.03e-14 75.5
MS.gene74229.t1 MTR_1g086350 26.132 287 143 10 83 356 19 249 1.09e-14 74.3
MS.gene74229.t1 MTR_2g435550 28.289 304 151 15 80 356 78 341 8.37e-14 72.4
MS.gene74229.t1 MTR_2g435550 28.289 304 151 15 80 356 92 355 8.40e-14 72.4
MS.gene74229.t1 MTR_8g102550 26.214 309 172 12 67 365 140 402 2.26e-13 71.2
MS.gene74229.t1 MTR_1g016620 30.534 262 113 13 74 301 62 288 2.73e-13 70.9
MS.gene74229.t1 MTR_7g021530 28.223 287 140 11 75 318 82 345 2.81e-13 71.2
MS.gene74229.t1 MTR_7g021530 28.223 287 140 11 75 318 123 386 3.09e-13 71.2
MS.gene74229.t1 MTR_7g021530 28.819 288 137 12 75 318 32 295 4.06e-13 70.5
MS.gene74229.t1 MTR_2g078760 28.125 224 111 7 72 290 110 288 6.73e-13 68.9
MS.gene74229.t1 MTR_2g435550 28.679 265 136 13 80 317 92 330 3.26e-12 67.8
MS.gene74229.t1 MTR_8g017240 27.451 255 147 8 80 318 86 318 5.64e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74229.t1 AT3G12620 75.132 378 87 3 3 373 4 381 0.0 587
MS.gene74229.t1 AT3G12620 75.132 378 87 3 3 373 4 381 0.0 587
MS.gene74229.t1 AT3G55050 72.606 376 96 3 1 370 1 375 0.0 564
MS.gene74229.t1 AT3G55050 72.606 376 96 3 1 370 1 375 0.0 564
MS.gene74229.t1 AT3G51370 66.757 370 120 1 4 370 3 372 0.0 521
MS.gene74229.t1 AT3G51370 66.757 370 120 1 4 370 3 372 0.0 521
MS.gene74229.t1 AT4G38520 64.191 377 128 2 1 370 1 377 1.75e-179 504
MS.gene74229.t1 AT4G38520 64.191 377 128 2 1 370 1 377 1.75e-179 504
MS.gene74229.t1 AT5G66080 68.286 350 104 2 28 370 29 378 7.12e-179 502
MS.gene74229.t1 AT5G02760 62.192 365 132 2 9 369 1 363 1.50e-159 452
MS.gene74229.t1 AT3G17090 59.877 324 120 3 33 355 45 359 8.15e-141 405
MS.gene74229.t1 AT3G51370 68.539 267 81 1 107 370 21 287 1.94e-133 384
MS.gene74229.t1 AT5G66080 67.037 270 83 1 107 370 5 274 1.32e-132 381
MS.gene74229.t1 AT4G33920 56.418 335 133 3 26 357 17 341 1.20e-130 380
MS.gene74229.t1 AT5G06750 53.779 344 143 3 33 368 43 378 2.25e-130 379
MS.gene74229.t1 AT5G06750 53.779 344 143 3 33 368 43 378 2.25e-130 379
MS.gene74229.t1 AT3G17090 56.364 275 110 3 33 306 45 310 1.68e-110 326
MS.gene74229.t1 AT4G33920 56.809 257 108 1 104 357 2 258 4.35e-101 301
MS.gene74229.t1 AT5G06750 54.647 269 114 2 108 368 28 296 1.34e-100 301
MS.gene74229.t1 AT4G33920 57.018 228 95 1 133 357 1 228 5.72e-90 272
MS.gene74229.t1 AT3G16560 30.769 312 173 6 80 354 175 480 6.28e-45 161
MS.gene74229.t1 AT3G16560 30.769 312 173 6 80 354 175 480 6.28e-45 161
MS.gene74229.t1 AT3G16560 30.769 312 173 6 80 354 175 480 6.28e-45 161
MS.gene74229.t1 AT2G46920 30.435 299 142 5 118 355 543 836 4.21e-37 143
MS.gene74229.t1 AT2G46920 30.435 299 142 5 118 355 543 836 4.21e-37 143
MS.gene74229.t1 AT2G46920 30.435 299 142 5 118 355 543 836 4.21e-37 143
MS.gene74229.t1 AT2G46920 30.435 299 142 5 118 355 543 836 4.21e-37 143
MS.gene74229.t1 AT1G07630 30.851 282 153 5 111 355 378 654 8.39e-37 141
MS.gene74229.t1 AT5G02400 31.560 282 154 5 108 355 390 666 1.22e-36 141
MS.gene74229.t1 AT3G16560 30.496 282 153 6 80 324 175 450 1.34e-36 138
MS.gene74229.t1 AT3G09400 32.463 268 141 7 119 355 384 642 5.92e-36 139
MS.gene74229.t1 AT1G07160 31.959 291 137 8 69 354 142 376 1.23e-35 134
MS.gene74229.t1 AT2G28890 30.961 281 149 6 115 355 371 646 2.44e-35 137
MS.gene74229.t1 AT2G35350 29.682 283 148 5 118 354 493 770 5.27e-34 134
MS.gene74229.t1 AT2G30020 30.000 290 143 7 69 354 159 392 1.01e-29 118
MS.gene74229.t1 AT2G40180 30.034 293 143 9 73 359 154 390 3.13e-29 117
MS.gene74229.t1 AT1G67820 31.884 276 132 10 36 301 68 297 6.31e-27 110
MS.gene74229.t1 AT1G67820 31.522 276 133 9 36 301 104 333 8.95e-27 111
MS.gene74229.t1 AT3G09400 31.224 237 123 7 119 324 384 611 2.66e-26 110
MS.gene74229.t1 AT4G31750 30.435 253 128 8 69 316 54 263 2.37e-24 102
MS.gene74229.t1 AT4G31750 30.435 253 128 8 69 316 54 263 2.42e-24 102
MS.gene74229.t1 AT4G31750 30.435 253 128 8 69 316 54 263 2.42e-24 102
MS.gene74229.t1 AT5G10740 31.765 255 125 10 69 318 54 264 1.22e-23 100
MS.gene74229.t1 AT5G10740 31.765 255 125 10 69 318 54 264 1.22e-23 100
MS.gene74229.t1 AT2G33700 29.703 303 136 10 70 360 113 350 4.19e-23 99.8
MS.gene74229.t1 AT1G43900 30.833 240 119 9 80 315 154 350 4.18e-22 97.1
MS.gene74229.t1 AT5G24940 31.746 252 123 10 72 318 57 264 4.75e-22 97.4
MS.gene74229.t1 AT4G28400 30.651 261 124 10 72 323 38 250 6.41e-21 91.7
MS.gene74229.t1 AT4G28400 30.651 261 124 10 72 323 60 272 1.12e-20 91.3
MS.gene74229.t1 AT4G08260 28.374 289 116 11 69 354 8 208 3.17e-20 88.6
MS.gene74229.t1 AT2G20630 28.520 277 138 11 53 320 40 265 1.03e-19 88.6
MS.gene74229.t1 AT1G78200 28.972 321 156 13 40 352 24 280 1.64e-19 88.2
MS.gene74229.t1 AT1G78200 28.972 321 156 13 40 352 24 280 1.64e-19 88.2
MS.gene74229.t1 AT1G78200 28.972 321 156 13 40 352 24 280 1.64e-19 88.2
MS.gene74229.t1 AT2G25620 27.027 296 140 11 79 359 127 361 1.72e-19 89.7
MS.gene74229.t1 AT2G25620 27.027 296 140 11 79 359 127 361 1.72e-19 89.7
MS.gene74229.t1 AT1G34750 27.245 323 160 13 40 354 26 281 1.81e-19 87.8
MS.gene74229.t1 AT1G34750 27.245 323 160 13 40 354 26 281 1.81e-19 87.8
MS.gene74229.t1 AT1G34750 27.245 323 160 13 40 354 26 281 1.81e-19 87.8
MS.gene74229.t1 AT2G20630 28.520 277 138 11 53 320 40 265 2.13e-19 87.8
MS.gene74229.t1 AT5G53140 25.839 298 155 8 70 364 123 357 3.08e-19 89.0
MS.gene74229.t1 AT5G53140 25.839 298 155 8 70 364 123 357 3.08e-19 89.0
MS.gene74229.t1 AT1G78200 29.514 288 138 11 69 352 9 235 9.46e-19 85.1
MS.gene74229.t1 AT1G78200 29.514 288 138 11 69 352 9 235 9.46e-19 85.1
MS.gene74229.t1 AT5G59220 29.583 240 122 9 80 309 148 350 1.55e-18 87.0
MS.gene74229.t1 AT1G07430 29.114 237 126 7 77 304 154 357 2.77e-18 86.3
MS.gene74229.t1 AT2G29380 29.289 239 126 8 80 308 112 317 6.62e-18 84.7
MS.gene74229.t1 AT1G72770 28.197 305 158 11 78 357 236 504 1.26e-17 84.7
MS.gene74229.t1 AT1G72770 28.197 305 158 11 78 357 236 504 1.26e-17 84.7
MS.gene74229.t1 AT1G72770 28.197 305 158 11 78 357 236 504 1.26e-17 84.7
MS.gene74229.t1 AT4G31860 25.746 268 125 8 79 302 51 288 1.63e-17 82.4
MS.gene74229.t1 AT4G26080 27.832 309 167 11 63 354 153 422 2.09e-17 83.6
MS.gene74229.t1 AT4G31860 25.746 268 125 8 79 302 51 288 3.37e-17 82.4
MS.gene74229.t1 AT5G57050 25.773 291 161 9 80 354 160 411 2.04e-16 80.9
MS.gene74229.t1 AT5G57050 25.773 291 161 9 80 354 120 371 2.49e-16 80.1
MS.gene74229.t1 AT3G16800 27.759 299 151 13 79 354 92 348 1.06e-15 78.2
MS.gene74229.t1 AT3G16800 27.759 299 151 13 79 354 92 348 1.06e-15 78.2
MS.gene74229.t1 AT3G16800 27.759 299 151 13 79 354 92 348 1.06e-15 78.2
MS.gene74229.t1 AT3G62260 30.638 235 113 9 80 302 27 223 1.21e-15 77.0
MS.gene74229.t1 AT5G51760 26.482 253 135 7 80 313 144 364 1.34e-15 78.2
MS.gene74229.t1 AT5G51760 26.482 253 135 7 80 313 114 334 1.35e-15 78.2
MS.gene74229.t1 AT1G22280 26.198 313 160 11 47 352 36 284 1.76e-15 76.6
MS.gene74229.t1 AT3G62260 30.638 235 113 9 80 302 116 312 2.24e-15 77.4
MS.gene74229.t1 AT2G25070 25.185 270 128 7 77 302 49 288 2.36e-15 77.0
MS.gene74229.t1 AT2G25070 25.185 270 128 7 77 302 49 288 2.36e-15 77.0
MS.gene74229.t1 AT3G62260 30.638 235 113 9 80 302 117 313 2.43e-15 77.4
MS.gene74229.t1 AT1G22280 26.861 309 157 11 47 352 36 278 2.74e-15 75.9
MS.gene74229.t1 AT5G26010 27.303 304 168 10 74 363 67 331 3.05e-15 76.6
MS.gene74229.t1 AT3G16800 29.778 225 108 9 142 354 67 253 7.43e-15 74.3
MS.gene74229.t1 AT3G16800 29.778 225 108 9 142 354 67 253 7.43e-15 74.3
MS.gene74229.t1 AT3G11410 28.049 246 123 9 80 306 137 347 1.07e-14 75.5
MS.gene74229.t1 AT1G34750 25.869 259 137 10 40 290 26 237 7.69e-14 71.2
MS.gene74229.t1 AT1G18030 25.874 286 159 8 54 320 83 334 8.38e-14 72.4
MS.gene74229.t1 AT3G05640 28.378 296 152 14 80 354 93 349 9.96e-14 72.0
MS.gene74229.t1 AT3G05640 28.378 296 152 14 80 354 93 349 9.96e-14 72.0
MS.gene74229.t1 AT3G05640 28.378 296 152 14 80 354 93 349 9.96e-14 72.0
MS.gene74229.t1 AT5G27930 26.885 305 149 15 80 353 92 353 1.70e-13 71.6
MS.gene74229.t1 AT5G27930 26.885 305 149 15 80 353 92 353 1.70e-13 71.6
MS.gene74229.t1 AT2G40860 25.806 279 150 9 80 354 325 550 3.21e-13 71.2
MS.gene74229.t1 AT2G40860 25.806 279 150 9 80 354 423 648 3.83e-13 71.2
MS.gene74229.t1 AT4G32950 26.510 298 168 13 74 360 66 323 4.27e-13 70.1
MS.gene74229.t1 AT3G15260 27.682 289 133 11 72 352 66 286 5.37e-13 69.3
MS.gene74229.t1 AT3G15260 27.682 289 133 11 72 352 66 286 5.37e-13 69.3
MS.gene74229.t1 AT2G34740 26.172 256 144 9 71 323 111 324 2.43e-12 67.8
MS.gene74229.t1 AT4G27800 24.551 334 200 10 23 325 23 335 2.58e-12 68.2
MS.gene74229.t1 AT4G27800 24.471 331 198 10 23 322 23 332 3.36e-12 67.4
MS.gene74229.t1 AT2G40860 26.754 228 129 7 80 303 423 616 3.52e-12 68.2
MS.gene74229.t1 AT4G27800 24.762 315 185 10 23 306 23 316 3.98e-12 67.0
MS.gene74229.t1 AT1G79630 29.858 211 102 9 153 354 222 395 9.29e-12 66.6
MS.gene74229.t1 AT1G79630 29.858 211 102 9 153 354 226 399 1.07e-11 66.6
MS.gene74229.t1 AT1G03590 27.742 310 156 15 79 355 89 363 1.61e-11 65.9
MS.gene74229.t1 AT1G79630 29.858 211 102 9 153 354 156 329 1.72e-11 65.9
MS.gene74229.t1 AT1G79630 29.858 211 102 9 153 354 156 329 1.72e-11 65.9
MS.gene74229.t1 AT1G79630 29.858 211 102 9 153 354 116 289 2.26e-11 65.1
MS.gene74229.t1 AT1G79630 29.858 211 102 9 153 354 116 289 2.26e-11 65.1
MS.gene74229.t1 AT1G79630 29.858 211 102 9 153 354 116 289 2.26e-11 65.1
MS.gene74229.t1 AT1G79630 29.858 211 102 9 153 354 116 289 2.26e-11 65.1
MS.gene74229.t1 AT2G05050 31.579 152 65 6 204 354 76 189 3.44e-11 62.4

Find 85 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACAAAGCGGGCAGTTTC+TGG 0.164257 1.2:+86363526 None:intergenic
ATTAAAGGAGGTGGTGATTT+TGG 0.205369 1.2:-86361505 MS.gene74229:CDS
TATTTGCATCAGATGGATTA+TGG 0.217881 1.2:-86361886 MS.gene74229:CDS
GCTCCGGCCGTTTCAATTAA+AGG 0.239097 1.2:-86361520 MS.gene74229:CDS
TTGTTATGGTATAAAGATTT+TGG 0.242228 1.2:-86363697 MS.gene74229:CDS
TTTGGTATTGGTATTGTAAA+TGG 0.282440 1.2:-86361487 MS.gene74229:CDS
TACTCTTGATATGTTTAAAT+AGG 0.299772 1.2:+86363497 None:intergenic
AGTTTCTGTACTTGTATTGT+TGG 0.321799 1.2:+86361932 None:intergenic
CACTACAATCTGTGGATCAT+TGG 0.323757 1.2:+86362310 None:intergenic
TCTATTGAGGGTGAAAATTC+TGG 0.335766 1.2:-86363745 MS.gene74229:CDS
CACCACTGTTAACAAAATTT+CGG 0.338437 1.2:-86361994 MS.gene74229:CDS
ATTTGCATCAGATGGATTAT+GGG 0.357369 1.2:-86361885 MS.gene74229:CDS
AGAGTTCACTTCAGAAAATC+AGG 0.359337 1.2:-86362618 MS.gene74229:CDS
ATAGGCGTTTCAAAGGGTTC+TGG 0.366966 1.2:+86361968 None:intergenic
CTCAATAGATGGCCTCCAAC+AGG 0.372346 1.2:+86363759 None:intergenic
GCGCGTGAAGGGTCTCATTC+AGG 0.386169 1.2:-86362270 MS.gene74229:intron
GGAGACTCACGAGCAGTGTT+AGG 0.392400 1.2:-86362434 MS.gene74229:CDS
GCTCCTCTCTTACAGATTCC+AGG 0.410655 1.2:+86362348 None:intergenic
ATACATTTCGTGGACAGTTC+TGG 0.411568 1.2:+86361828 None:intergenic
GCATGGTATCTGCTACAATT+AGG 0.417877 1.2:-86363795 MS.gene74229:CDS
GTATTGTTGGTTCAGCTTTG+AGG 0.418489 1.2:+86361945 None:intergenic
CAGGCTAGCATTGTGCTCAT+AGG 0.434489 1.2:+86362367 None:intergenic
TGTTCAAGCAAACAATTTGT+TGG 0.434692 1.2:-86363641 MS.gene74229:CDS
GATCAATGTCAAGTTGAATC+AGG 0.439614 1.2:-86363616 MS.gene74229:CDS
TTTCTTATATTTGCATCAGA+TGG 0.442628 1.2:-86361893 MS.gene74229:CDS
ACCACCTCCTTTAATTGAAA+CGG 0.447020 1.2:+86361513 None:intergenic
GAAACTGCCCGCTTTGTCAA+TGG 0.450020 1.2:-86363523 MS.gene74229:CDS
AATTAGAGTCATACATTTCG+TGG 0.453536 1.2:+86361818 None:intergenic
ACTACAATCTGTGGATCATT+GGG 0.457344 1.2:+86362311 None:intergenic
GTCAGTTGGATCTTGCTGTT+TGG 0.461911 1.2:-86362498 MS.gene74229:CDS
ACTTCCTTCAATTGAGCTCA+TGG 0.467701 1.2:+86363591 None:intergenic
GACGTTCCGATGGTTTGTTA+TGG 0.468753 1.2:-86363711 MS.gene74229:CDS
CTATGAGCACAATGCTAGCC+TGG 0.471192 1.2:-86362366 MS.gene74229:CDS
TAGTGATGAAGCACACGGTC+TGG 0.471323 1.2:-86362292 MS.gene74229:CDS
GTTGGTTCAGCTTTGAGGAT+AGG 0.471519 1.2:+86361950 None:intergenic
CTGTTGGAGGCCATCTATTG+AGG 0.471879 1.2:-86363758 MS.gene74229:CDS
TAGGCGTTTCAAAGGGTTCT+GGG 0.478115 1.2:+86361969 None:intergenic
TGGTAGGTGTAATATATAGT+GGG 0.484611 1.2:-86362478 MS.gene74229:CDS
ACGAGCAGTGTTAGGTAGAC+TGG 0.485877 1.2:-86362426 MS.gene74229:CDS
GTTGGATCTTGCTGTTTGGT+AGG 0.488410 1.2:-86362494 MS.gene74229:CDS
TTCGCATAATCCACGTGCTC+CGG 0.499440 1.2:-86361536 MS.gene74229:CDS
TTGGTAGGTGTAATATATAG+TGG 0.504051 1.2:-86362479 MS.gene74229:CDS
GCCGTTTCAATTAAAGGAGG+TGG 0.506500 1.2:-86361514 MS.gene74229:CDS
TTCAGATGGTGATTTCTCAA+TGG 0.524527 1.2:-86363668 MS.gene74229:CDS
TCAAGCAAACAATTTGTTGG+AGG 0.525470 1.2:-86363638 MS.gene74229:CDS
GGCGAATTGTGTCACCCTGT+TGG 0.525819 1.2:-86363774 MS.gene74229:CDS
TTTGAGGATAGGCGTTTCAA+AGG 0.530554 1.2:+86361961 None:intergenic
TGTTGGAGGCCATCTATTGA+GGG 0.532239 1.2:-86363757 MS.gene74229:CDS
ATTGAAACGGCCGGAGCACG+TGG 0.540771 1.2:+86361526 None:intergenic
TTGTAGATTTCAAGATCCAT+TGG 0.542867 1.2:-86362052 MS.gene74229:intron
CTTTAGATAAGCGTCGCCAA+TGG 0.546139 1.2:+86362036 None:intergenic
GGACCCATGAGCTCAATTGA+AGG 0.549310 1.2:-86363595 MS.gene74229:CDS
ACATTTCCATGACGACATAA+CGG 0.553652 1.2:-86361578 MS.gene74229:CDS
TTGAGGATAGGCGTTTCAAA+GGG 0.554445 1.2:+86361962 None:intergenic
CTTCCTTCAATTGAGCTCAT+GGG 0.556342 1.2:+86363592 None:intergenic
GAAAATTCTGGCGATGCAAG+TGG 0.556908 1.2:-86363733 MS.gene74229:CDS
GATGCAAGTGGACGTTCCGA+TGG 0.561098 1.2:-86363721 MS.gene74229:CDS
CTATAACCGTTATGTCGTCA+TGG 0.567213 1.2:+86361572 None:intergenic
CACACGGTCTGGCGCGTGAA+GGG 0.569921 1.2:-86362281 MS.gene74229:CDS
GAGCTCTATATTGCAAATGC+AGG 0.575306 1.2:-86362455 MS.gene74229:CDS
CCTCCTTTAATTGAAACGGC+CGG 0.583085 1.2:+86361517 None:intergenic
GCACACGGTCTGGCGCGTGA+AGG 0.583272 1.2:-86362282 MS.gene74229:CDS
TGCTTCATCACTACAATCTG+TGG 0.585449 1.2:+86362302 None:intergenic
TAGGCGTCCATTGACAAAGC+GGG 0.585929 1.2:+86363516 None:intergenic
GAATTGTGTCACCCTGTTGG+AGG 0.594098 1.2:-86363771 MS.gene74229:CDS
GGTAGGTGTAATATATAGTG+GGG 0.597591 1.2:-86362477 MS.gene74229:CDS
GAAAAGAGAAATGAGATACT+CGG 0.606130 1.2:-86361632 MS.gene74229:CDS
AAACCACAGATAGCGTCAGT+TGG 0.606742 1.2:-86362512 MS.gene74229:CDS
GTTGGTGTATATGACGGGCA+TGG 0.608084 1.2:-86363553 MS.gene74229:CDS
TTTATACCATAACAAACCAT+CGG 0.613192 1.2:+86363705 None:intergenic
TCGCTCTTGTAAAGAAGCAG+TGG 0.615921 1.2:-86362541 MS.gene74229:CDS
AATATAAGAAATTGATCACA+AGG 0.622869 1.2:+86361905 None:intergenic
TCAATAGATGGCCTCCAACA+GGG 0.624913 1.2:+86363760 None:intergenic
TAGCCTGGAATCTGTAAGAG+AGG 0.632952 1.2:-86362351 MS.gene74229:CDS
GAGTTCACTTCAGAAAATCA+GGG 0.638352 1.2:-86362617 MS.gene74229:CDS
GGAAATGTCTTCGTACCCCA+CGG 0.645830 1.2:+86361593 None:intergenic
GATCCAACTGACGCTATCTG+TGG 0.645990 1.2:+86362509 None:intergenic
AGTGTTAGGTAGACTGGACG+AGG 0.648197 1.2:-86362420 MS.gene74229:CDS
CCGGCCGTTTCAATTAAAGG+AGG 0.675949 1.2:-86361517 MS.gene74229:CDS
ATAGGCGTCCATTGACAAAG+CGG 0.707051 1.2:+86363515 None:intergenic
GGCTACAAAAGAGATTAAAG+CGG 0.707834 1.2:-86362399 MS.gene74229:CDS
TCAATTGAAGGAAGTCCTCG+AGG 0.723635 1.2:-86363583 MS.gene74229:CDS
ACACCAACAAAAGTACCTCG+AGG 0.740115 1.2:+86363568 None:intergenic
GATTGTAGTGATGAAGCACA+CGG 0.742615 1.2:-86362297 MS.gene74229:CDS
GGTGTATATGACGGGCATGG+AGG 0.742632 1.2:-86363550 MS.gene74229:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAGTTTTGTAAAATC+TGG + chr1.2:86362440-86362459 None:intergenic 15.0%
!!! AAAAAAGTTTTGTAAAATCT+GGG + chr1.2:86362439-86362458 None:intergenic 15.0%
!!! ATATTTGTCTTTTATCTTTA+TGG - chr1.2:86362258-86362277 MS.gene74229:intron 15.0%
!!! ATTTTTATAGATGAAAAGAA+GGG + chr1.2:86361934-86361953 None:intergenic 15.0%
!!! TTAGAAAATTTGTTTTCAAA+CGG - chr1.2:86362374-86362393 MS.gene74229:CDS 15.0%
!! AATATAAGAAATTGATCACA+AGG + chr1.2:86363374-86363393 None:intergenic 20.0%
!! AATCATACACTGAAATAAAT+TGG + chr1.2:86362147-86362166 None:intergenic 20.0%
!! ATCATACACTGAAATAAATT+GGG + chr1.2:86362146-86362165 None:intergenic 20.0%
!! ATGCAATTATTTGTATTACA+TGG + chr1.2:86362090-86362109 None:intergenic 20.0%
!! TAAAACAAAATTATGTTGCA+GGG + chr1.2:86361824-86361843 None:intergenic 20.0%
!! TACTCTTGATATGTTTAAAT+AGG + chr1.2:86361782-86361801 None:intergenic 20.0%
!! TTAAAACAAAATTATGTTGC+AGG + chr1.2:86361825-86361844 None:intergenic 20.0%
!!! GAAAACAAATTTTCTAATCA+GGG + chr1.2:86362371-86362390 None:intergenic 20.0%
!!! GATTTTTATAGATGAAAAGA+AGG + chr1.2:86361935-86361954 None:intergenic 20.0%
!!! TGAAAACAAATTTTCTAATC+AGG + chr1.2:86362372-86362391 None:intergenic 20.0%
!!! TTGTTATGGTATAAAGATTT+TGG - chr1.2:86361579-86361598 MS.gene74229:CDS 20.0%
! AGTATTGTAAGATGACATAA+GGG - chr1.2:86362563-86362582 MS.gene74229:CDS 25.0%
! ATATTCATGCTTGTCTTAAT+TGG - chr1.2:86361856-86361875 MS.gene74229:CDS 25.0%
! TATCTTTATGGAATTTCATG+TGG - chr1.2:86362270-86362289 MS.gene74229:intron 25.0%
! TTTATACCATAACAAACCAT+CGG + chr1.2:86361574-86361593 None:intergenic 25.0%
! TTTCTTATATTTGCATCAGA+TGG - chr1.2:86363383-86363402 MS.gene74229:intron 25.0%
!! AAGTATTGTAAGATGACATA+AGG - chr1.2:86362562-86362581 MS.gene74229:CDS 25.0%
!! TTTTTGTGTATTACTTTACC+TGG - chr1.2:86362598-86362617 MS.gene74229:CDS 25.0%
!!! AGTTCCTCTAAAACTATTTA+GGG + chr1.2:86361979-86361998 None:intergenic 25.0%
!!! ATTGGATTCTTGTTATTTTG+CGG - chr1.2:86361874-86361893 MS.gene74229:CDS 25.0%
!!! TAAATAGTTTTAGAGGAACT+CGG - chr1.2:86361979-86361998 MS.gene74229:CDS 25.0%
!!! TGTTATCACTAAAGCATTTT+TGG - chr1.2:86362694-86362713 MS.gene74229:intron 25.0%
!!! TTTGGTATTGGTATTGTAAA+TGG - chr1.2:86363789-86363808 MS.gene74229:CDS 25.0%
AATTAGAGTCATACATTTCG+TGG + chr1.2:86363461-86363480 None:intergenic 30.0%
AGTTTCTGTACTTGTATTGT+TGG + chr1.2:86363347-86363366 None:intergenic 30.0%
ATCATCTGCATGAGTTAAAT+AGG + chr1.2:86362027-86362046 None:intergenic 30.0%
ATTCAGAAATCATATGAACC+AGG + chr1.2:86362619-86362638 None:intergenic 30.0%
ATTTAACTCATGCAGATGAT+GGG - chr1.2:86362027-86362046 MS.gene74229:CDS 30.0%
CACCACTGTTAACAAAATTT+CGG - chr1.2:86363282-86363301 MS.gene74229:intron 30.0%
GAAAAGAGAAATGAGATACT+CGG - chr1.2:86363644-86363663 MS.gene74229:CDS 30.0%
TATTTAACTCATGCAGATGA+TGG - chr1.2:86362026-86362045 MS.gene74229:CDS 30.0%
TCAAAGAGTAACAAATAAGC+AGG + chr1.2:86362540-86362559 None:intergenic 30.0%
TCGATCAAATTGAAGTATAG+TGG - chr1.2:86362472-86362491 MS.gene74229:CDS 30.0%
TGGTAGGTGTAATATATAGT+GGG - chr1.2:86362798-86362817 MS.gene74229:intron 30.0%
TGTTCAAGCAAACAATTTGT+TGG - chr1.2:86361635-86361654 MS.gene74229:CDS 30.0%
TTGGTAGGTGTAATATATAG+TGG - chr1.2:86362797-86362816 MS.gene74229:intron 30.0%
TTGTAGATTTCAAGATCCAT+TGG - chr1.2:86363224-86363243 MS.gene74229:intron 30.0%
! ACTTTTGTTGGTGTATATGA+CGG - chr1.2:86361717-86361736 MS.gene74229:intron 30.0%
! ATTTAGAGCTGACAGAAATA+GGG + chr1.2:86363507-86363526 None:intergenic 30.0%
! GCACATCAACTCATAATTTT+AGG + chr1.2:86363126-86363145 None:intergenic 30.0%
!! ATTTGCATCAGATGGATTAT+GGG - chr1.2:86363391-86363410 MS.gene74229:intron 30.0%
!! CTCTAAAACTATTTAGGGTA+AGG + chr1.2:86361974-86361993 None:intergenic 30.0%
!! GAGTTCCTCTAAAACTATTT+AGG + chr1.2:86361980-86361999 None:intergenic 30.0%
!! TATTTGCATCAGATGGATTA+TGG - chr1.2:86363390-86363409 MS.gene74229:intron 30.0%
!!! CTTACCCTAAATAGTTTTAG+AGG - chr1.2:86361972-86361991 MS.gene74229:CDS 30.0%
ACATTTCCATGACGACATAA+CGG - chr1.2:86363698-86363717 MS.gene74229:CDS 35.0%
ACCACCTCCTTTAATTGAAA+CGG + chr1.2:86363766-86363785 None:intergenic 35.0%
ACTACAATCTGTGGATCATT+GGG + chr1.2:86362968-86362987 None:intergenic 35.0%
AGAGTTCACTTCAGAAAATC+AGG - chr1.2:86362658-86362677 MS.gene74229:intron 35.0%
AGTAACAAATAAGCAGGCAA+AGG + chr1.2:86362534-86362553 None:intergenic 35.0%
ATTGTAAGATGACATAAGGG+AGG - chr1.2:86362566-86362585 MS.gene74229:CDS 35.0%
GAAAAATCCCTCTATAGCTA+AGG + chr1.2:86361912-86361931 None:intergenic 35.0%
GAGTTCACTTCAGAAAATCA+GGG - chr1.2:86362659-86362678 MS.gene74229:intron 35.0%
GGCTACAAAAGAGATTAAAG+CGG - chr1.2:86362877-86362896 MS.gene74229:intron 35.0%
GGTAGGTGTAATATATAGTG+GGG - chr1.2:86362799-86362818 MS.gene74229:intron 35.0%
TAAGCAGGCAAAGGAATAAA+AGG + chr1.2:86362525-86362544 None:intergenic 35.0%
TCTATTGAGGGTGAAAATTC+TGG - chr1.2:86361531-86361550 MS.gene74229:CDS 35.0%
TTCAGATGGTGATTTCTCAA+TGG - chr1.2:86361608-86361627 MS.gene74229:CDS 35.0%
! AGCCGAAATTTTGTTAACAG+TGG + chr1.2:86363287-86363306 None:intergenic 35.0%
! ATTAAAGGAGGTGGTGATTT+TGG - chr1.2:86363771-86363790 MS.gene74229:CDS 35.0%
! ATTTTGTTAACAGTGGTGCT+TGG + chr1.2:86363280-86363299 None:intergenic 35.0%
! CTTTTGTTGGTGTATATGAC+GGG - chr1.2:86361718-86361737 MS.gene74229:intron 35.0%
! GAATTTTCACCCTCAATAGA+TGG + chr1.2:86361531-86361550 None:intergenic 35.0%
! GATTTAGAGCTGACAGAAAT+AGG + chr1.2:86363508-86363527 None:intergenic 35.0%
! TTAGGGTAAGGTTTTTCTAG+TGG + chr1.2:86361962-86361981 None:intergenic 35.0%
!! AAATTGGGCTTTTAGAAACG+TGG + chr1.2:86362131-86362150 None:intergenic 35.0%
!! ATTTTAGGAAACACCAACAC+AGG + chr1.2:86363111-86363130 None:intergenic 35.0%
!! GATCAATGTCAAGTTGAATC+AGG - chr1.2:86361660-86361679 MS.gene74229:CDS 35.0%
!! GATTTTGGAAGACATTCAGA+TGG - chr1.2:86361594-86361613 MS.gene74229:CDS 35.0%
!! TCAAGCAAACAATTTGTTGG+AGG - chr1.2:86361638-86361657 MS.gene74229:CDS 35.0%
!! TTATGAGTTGATGTGCATGT+TGG - chr1.2:86363130-86363149 MS.gene74229:intron 35.0%
ACAAAATCAGTTAATCCCCG+TGG + chr1.2:86362330-86362349 None:intergenic 40.0%
ACCTTGATCCTTAGCTATAG+AGG - chr1.2:86361901-86361920 MS.gene74229:CDS 40.0%
ACTTCCTTCAATTGAGCTCA+TGG + chr1.2:86361688-86361707 None:intergenic 40.0%
ACTTGAAAAAGATCGACCGT+GGG - chr1.2:86363667-86363686 MS.gene74229:CDS 40.0%
ATACATTTCGTGGACAGTTC+TGG + chr1.2:86363451-86363470 None:intergenic 40.0%
ATGGATGGTACTAAGTTCCA+CGG - chr1.2:86362310-86362329 MS.gene74229:CDS 40.0%
CACTACAATCTGTGGATCAT+TGG + chr1.2:86362969-86362988 None:intergenic 40.0%
CCTTGATCCTTAGCTATAGA+GGG - chr1.2:86361902-86361921 MS.gene74229:CDS 40.0%
CTATAACCGTTATGTCGTCA+TGG + chr1.2:86363707-86363726 None:intergenic 40.0%
CTTCCTTCAATTGAGCTCAT+GGG + chr1.2:86361687-86361706 None:intergenic 40.0%
GAGCTCTATATTGCAAATGC+AGG - chr1.2:86362821-86362840 MS.gene74229:intron 40.0%
GATTGTAGTGATGAAGCACA+CGG - chr1.2:86362979-86362998 MS.gene74229:intron 40.0%
TAAGATGACATAAGGGAGGA+GGG - chr1.2:86362570-86362589 MS.gene74229:CDS 40.0%
TAGTACCATCCATCTTCCAT+TGG + chr1.2:86362303-86362322 None:intergenic 40.0%
TGCTTCATCACTACAATCTG+TGG + chr1.2:86362977-86362996 None:intergenic 40.0%
TTCATGTGGATGACATCCAA+TGG - chr1.2:86362284-86362303 MS.gene74229:CDS 40.0%
TTGAGGATAGGCGTTTCAAA+GGG + chr1.2:86363317-86363336 None:intergenic 40.0%
TTTGAGGATAGGCGTTTCAA+AGG + chr1.2:86363318-86363337 None:intergenic 40.0%
! GTTTTCAAACGGTCACTAGA+TGG - chr1.2:86362385-86362404 MS.gene74229:CDS 40.0%
!! GTATTGTTGGTTCAGCTTTG+AGG + chr1.2:86363334-86363353 None:intergenic 40.0%
AAACCACAGATAGCGTCAGT+TGG - chr1.2:86362764-86362783 MS.gene74229:intron 45.0%
AACTGTGCTGTCTGTTGTAC+AGG - chr1.2:86363575-86363594 MS.gene74229:CDS 45.0%
AAGATGACATAAGGGAGGAG+GGG - chr1.2:86362571-86362590 MS.gene74229:CDS 45.0%
ACTGTGCTGTCTGTTGTACA+GGG - chr1.2:86363576-86363595 MS.gene74229:CDS 45.0%
ATAGGCGTCCATTGACAAAG+CGG + chr1.2:86361764-86361783 None:intergenic 45.0%
ATAGGCGTTTCAAAGGGTTC+TGG + chr1.2:86363311-86363330 None:intergenic 45.0%
CACACATTCAATGCCTGTGT+TGG - chr1.2:86363095-86363114 MS.gene74229:intron 45.0%
CCCTCTATAGCTAAGGATCA+AGG + chr1.2:86361905-86361924 None:intergenic 45.0%
CCTCCTTTAATTGAAACGGC+CGG + chr1.2:86363762-86363781 None:intergenic 45.0%
CTTGAAAAAGATCGACCGTG+GGG - chr1.2:86363668-86363687 MS.gene74229:CDS 45.0%
GAAAATTCTGGCGATGCAAG+TGG - chr1.2:86361543-86361562 MS.gene74229:CDS 45.0%
GACATCCAATGGAAGATGGA+TGG - chr1.2:86362295-86362314 MS.gene74229:CDS 45.0%
GACTTGAAAAAGATCGACCG+TGG - chr1.2:86363666-86363685 MS.gene74229:CDS 45.0%
GCCGTTTCAATTAAAGGAGG+TGG - chr1.2:86363762-86363781 MS.gene74229:CDS 45.0%
GGATGACATCCAATGGAAGA+TGG - chr1.2:86362291-86362310 MS.gene74229:CDS 45.0%
GTAAGATGACATAAGGGAGG+AGG - chr1.2:86362569-86362588 MS.gene74229:CDS 45.0%
TAGCCTGGAATCTGTAAGAG+AGG - chr1.2:86362925-86362944 MS.gene74229:intron 45.0%
TAGGCGTTTCAAAGGGTTCT+GGG + chr1.2:86363310-86363329 None:intergenic 45.0%
TCAATAGATGGCCTCCAACA+GGG + chr1.2:86361519-86361538 None:intergenic 45.0%
TCAATTGAAGGAAGTCCTCG+AGG - chr1.2:86361693-86361712 MS.gene74229:intron 45.0%
TCGCTCTTGTAAAGAAGCAG+TGG - chr1.2:86362735-86362754 MS.gene74229:intron 45.0%
TGGATGGTACTAAGTTCCAC+GGG - chr1.2:86362311-86362330 MS.gene74229:CDS 45.0%
TGTTGGAGGCCATCTATTGA+GGG - chr1.2:86361519-86361538 MS.gene74229:CDS 45.0%
! CTTTAGATAAGCGTCGCCAA+TGG + chr1.2:86363243-86363262 None:intergenic 45.0%
!! ACACCAACAAAAGTACCTCG+AGG + chr1.2:86361711-86361730 None:intergenic 45.0%
!! AGTCCTCGAGGTACTTTTGT+TGG - chr1.2:86361705-86361724 MS.gene74229:intron 45.0%
!! CTCATGCAGATGATGGGATA+TGG - chr1.2:86362033-86362052 MS.gene74229:CDS 45.0%
!! GACGTTCCGATGGTTTGTTA+TGG - chr1.2:86361565-86361584 MS.gene74229:CDS 45.0%
!! GGAGGTGGTGATTTTGGTAT+TGG - chr1.2:86363777-86363796 MS.gene74229:CDS 45.0%
!! GTCAGTTGGATCTTGCTGTT+TGG - chr1.2:86362778-86362797 MS.gene74229:intron 45.0%
!! GTTGGATCTTGCTGTTTGGT+AGG - chr1.2:86362782-86362801 MS.gene74229:intron 45.0%
!! GTTGGTTCAGCTTTGAGGAT+AGG + chr1.2:86363329-86363348 None:intergenic 45.0%
!! TGAGTTGATGTGCATGTTGG+AGG - chr1.2:86363133-86363152 MS.gene74229:intron 45.0%
ACGAGCAGTGTTAGGTAGAC+TGG - chr1.2:86362850-86362869 MS.gene74229:intron 50.0%
AGATGACATAAGGGAGGAGG+GGG - chr1.2:86362572-86362591 MS.gene74229:CDS 50.0%
AGTGTTAGGTAGACTGGACG+AGG - chr1.2:86362856-86362875 MS.gene74229:intron 50.0%
CAGGCTAGCATTGTGCTCAT+AGG + chr1.2:86362912-86362931 None:intergenic 50.0%
CCGGCCGTTTCAATTAAAGG+AGG - chr1.2:86363759-86363778 MS.gene74229:CDS 50.0%
CTATGAGCACAATGCTAGCC+TGG - chr1.2:86362910-86362929 MS.gene74229:intron 50.0%
CTCAATAGATGGCCTCCAAC+AGG + chr1.2:86361520-86361539 None:intergenic 50.0%
CTGTTGGAGGCCATCTATTG+AGG - chr1.2:86361518-86361537 MS.gene74229:CDS 50.0%
GAAACTGCCCGCTTTGTCAA+TGG - chr1.2:86361753-86361772 MS.gene74229:intron 50.0%
GAATTGTGTCACCCTGTTGG+AGG - chr1.2:86361505-86361524 MS.gene74229:CDS 50.0%
GATCCAACTGACGCTATCTG+TGG + chr1.2:86362770-86362789 None:intergenic 50.0%
GCTCCGGCCGTTTCAATTAA+AGG - chr1.2:86363756-86363775 MS.gene74229:CDS 50.0%
GCTCCTCTCTTACAGATTCC+AGG + chr1.2:86362931-86362950 None:intergenic 50.0%
GGAAATGTCTTCGTACCCCA+CGG + chr1.2:86363686-86363705 None:intergenic 50.0%
GGACCCATGAGCTCAATTGA+AGG - chr1.2:86361681-86361700 MS.gene74229:CDS 50.0%
GGATGGTACTAAGTTCCACG+GGG - chr1.2:86362312-86362331 MS.gene74229:CDS 50.0%
TAGGCGTCCATTGACAAAGC+GGG + chr1.2:86361763-86361782 None:intergenic 50.0%
TAGTGATGAAGCACACGGTC+TGG - chr1.2:86362984-86363003 MS.gene74229:intron 50.0%
TTCGCATAATCCACGTGCTC+CGG - chr1.2:86363740-86363759 MS.gene74229:CDS 50.0%
TTGACAAAGCGGGCAGTTTC+TGG + chr1.2:86361753-86361772 None:intergenic 50.0%
!! GTTGGTGTATATGACGGGCA+TGG - chr1.2:86361723-86361742 MS.gene74229:intron 50.0%
GATGCAAGTGGACGTTCCGA+TGG - chr1.2:86361555-86361574 MS.gene74229:CDS 55.0%
GGAGACTCACGAGCAGTGTT+AGG - chr1.2:86362842-86362861 MS.gene74229:intron 55.0%
GGCGAATTGTGTCACCCTGT+TGG - chr1.2:86361502-86361521 MS.gene74229:CDS 55.0%
!! GGTGTATATGACGGGCATGG+AGG - chr1.2:86361726-86361745 MS.gene74229:intron 55.0%
ATTGAAACGGCCGGAGCACG+TGG + chr1.2:86363753-86363772 None:intergenic 60.0%
GCGCGTGAAGGGTCTCATTC+AGG - chr1.2:86363006-86363025 MS.gene74229:intron 60.0%
CACACGGTCTGGCGCGTGAA+GGG - chr1.2:86362995-86363014 MS.gene74229:intron 65.0%
GCACACGGTCTGGCGCGTGA+AGG - chr1.2:86362994-86363013 MS.gene74229:intron 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 86361483 86363815 86361483 ID=MS.gene74229
chr1.2 mRNA 86361483 86363815 86361483 ID=MS.gene74229.t1;Parent=MS.gene74229
chr1.2 exon 86363500 86363815 86363500 ID=MS.gene74229.t1.exon1;Parent=MS.gene74229.t1
chr1.2 CDS 86363500 86363815 86363500 ID=cds.MS.gene74229.t1;Parent=MS.gene74229.t1
chr1.2 exon 86362271 86362638 86362271 ID=MS.gene74229.t1.exon2;Parent=MS.gene74229.t1
chr1.2 CDS 86362271 86362638 86362271 ID=cds.MS.gene74229.t1;Parent=MS.gene74229.t1
chr1.2 exon 86361832 86362068 86361832 ID=MS.gene74229.t1.exon3;Parent=MS.gene74229.t1
chr1.2 CDS 86361832 86362068 86361832 ID=cds.MS.gene74229.t1;Parent=MS.gene74229.t1
chr1.2 exon 86361483 86361701 86361483 ID=MS.gene74229.t1.exon4;Parent=MS.gene74229.t1
chr1.2 CDS 86361483 86361701 86361483 ID=cds.MS.gene74229.t1;Parent=MS.gene74229.t1
Gene Sequence

>MS.gene74229

ATGGTATCTGCTACAATTAGGCGAATTGTGTCACCCTGTTGGAGGCCATCTATTGAGGGTGAAAATTCTGGCGATGCAAGTGGACGTTCCGATGGTTTGTTATGGTATAAAGATTTTGGAAGACATTCAGATGGTGATTTCTCAATGGCTGTTGTTCAAGCAAACAATTTGTTGGAGGATCAATGTCAAGTTGAATCAGGACCCATGAGCTCAATTGAAGGAAGTCCTCGAGGTACTTTTGTTGGTGTATATGACGGGCATGGAGGTCCAGAAACTGCCCGCTTTGTCAATGGACGCCTATTTAAACATATCAAGAGTATGCTCTTCTTTTCTCGTCACCCTGCAACATAATTTTGTTTTAATAGATAGATATATATTCATGCTTGTCTTAATTGGATTCTTGTTATTTTGCGGCATGACCTTGATCCTTAGCTATAGAGGGATTTTTCCCTTCTTTTCATCTATAAAAATCACGTCCACTAGAAAAACCTTACCCTAAATAGTTTTAGAGGAACTCGGATTTTTGTTAAGAGTTGTTAAACCTATTTAACTCATGCAGATGATGGGATATGGATTAGTTGTTGTTTTTATTAGATGCTAAGAACCATGTAATACAAATAATTGCATATTTTGATTCTTTTTTCTCCACGTTTCTAAAAGCCCAATTTATTTCAGTGTATGATTGTTTTTGTCTTTATTGTTCAAGATCTTTATGATATTCACAATTTATTTCAATGTATGATTGTTTTTGTTTTTATTGTTCAAGATCTTTATGATATTTGTCTTTTATCTTTATGGAATTTCATGTGGATGACATCCAATGGAAGATGGATGGTACTAAGTTCCACGGGGATTAACTGATTTTGTGATCTAATAACTTGATATCCCTGATTAGAAAATTTGTTTTCAAACGGTCACTAGATGGTGCTTGCTTACTCTTTGTAACTAATAATCCCAGATTTTACAAAACTTTTTTTTATGTATTAAACTCGATCAAATTGAAGTATAGTGGCGTGAAGTATTGATAAAATTCAGATTACCTTTTATTCCTTTGCCTGCTTATTTGTTACTCTTTGAAAAAGTATTGTAAGATGACATAAGGGAGGAGGGGGTGATTTTTGTGTATTACTTTACCTGGTTCATATGATTTCTGAATTCTCAGCTGATCTATATGCAGAGTTCACTTCAGAAAATCAGGGAATGTCAGCTGATGTTATCACTAAAGCATTTTTGGCTACAGAAGAAGAATTTCTCGCTCTTGTAAAGAAGCAGTGGCAACACAAACCACAGATAGCGTCAGTTGGATCTTGCTGTTTGGTAGGTGTAATATATAGTGGGGAGCTCTATATTGCAAATGCAGGAGACTCACGAGCAGTGTTAGGTAGACTGGACGAGGCTACAAAAGAGATTAAAGCGGTTCAACTATCCTATGAGCACAATGCTAGCCTGGAATCTGTAAGAGAGGAGCTACGCTCATTGCATCCCAATGATCCACAGATTGTAGTGATGAAGCACACGGTCTGGCGCGTGAAGGGTCTCATTCAGGTGACTATTTAAAAGCTTTATTTCTCAAGTTTCATTTTCTCTTTTCATGTAATGTAAGATACACGTGCACACATTCAATGCCTGTGTTGGTGTTTCCTAAAATTATGAGTTGATGTGCATGTTGGAGGTATGTGTTCATGAACATGAATGTCTTCTGAAAATCATAGAGTTGTGTTGTTTAGATTGTTTATTTACATTGTAGATTTCAAGATCCATTGGCGACGCTTATCTAAAGAAGAAAGAGTTTAACCAAGCACCACTGTTAACAAAATTTCGGCTCCCAGAACCCTTTGAAACGCCTATCCTCAAAGCTGAACCAACAATACAAGTACAGAAACTACAACCTTGTGATCAATTTCTTATATTTGCATCAGATGGATTATGGGAGCACTTGAGCAATCAAGAAGCTGTTGACATTGTCCAGAACTGTCCACGAAATGTATGACTCTAATTTAGTTGAGTTTTTTTTTTTTCTTCCCTATTTCTGTCAGCTCTAAATCTTCTTCTGATCTGCTATATTCAATGTTCACTTCATTGTATAAACAACAACTGTGCTGTCTGTTGTACAGGGAGTAGCGAAGAAACTGATCAAAGCAGCGCTTTGTGAAGCTGCAAAGAAAAGAGAAATGAGATACTCGGACTTGAAAAAGATCGACCGTGGGGTACGAAGACATTTCCATGACGACATAACGGTTATAGTTGTGTATCTTGATTCGCATAATCCACGTGCTCCGGCCGTTTCAATTAAAGGAGGTGGTGATTTTGGTATTGGTATTGTAAATGGCTAG
Protein sequence

>MS.gene74229.t1

MVSATIRRIVSPCWRPSIEGENSGDASGRSDGLLWYKDFGRHSDGDFSMAVVQANNLLEDQCQVESGPMSSIEGSPRGTFVGVYDGHGGPETARFVNGRLFKHIKKFTSENQGMSADVITKAFLATEEEFLALVKKQWQHKPQIASVGSCCLVGVIYSGELYIANAGDSRAVLGRLDEATKEIKAVQLSYEHNASLESVREELRSLHPNDPQIVVMKHTVWRVKGLIQISRSIGDAYLKKKEFNQAPLLTKFRLPEPFETPILKAEPTIQVQKLQPCDQFLIFASDGLWEHLSNQEAVDIVQNCPRNGVAKKLIKAALCEAAKKREMRYSDLKKIDRGVRRHFHDDITVIVVYLDSHNPRAPAVSIKGGGDFGIGIVNG