AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56672

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56672.t1 MTR_5g082070 99.308 289 2 0 1 289 1 289 0.0 582
MS.gene56672.t1 MTR_3g070210 92.388 289 22 0 1 289 1 289 0.0 547
MS.gene56672.t1 MTR_8g098375 87.063 286 35 1 4 289 3 286 0.0 512
MS.gene56672.t1 MTR_5g010150 85.315 286 40 1 4 289 3 286 3.11e-180 498
MS.gene56672.t1 MTR_3g070210 91.603 262 22 0 1 262 1 262 4.24e-178 492
MS.gene56672.t1 MTR_4g415300 81.034 290 53 2 1 289 1 289 1.34e-173 481
MS.gene56672.t1 MTR_3g070210 90.610 213 20 0 1 213 1 213 1.60e-142 400
MS.gene56672.t1 MTR_7g101190 72.868 258 63 2 32 283 16 272 1.54e-135 385
MS.gene56672.t1 MTR_2g094270 68.939 264 74 2 32 288 17 279 8.03e-134 380
MS.gene56672.t1 MTR_8g098375 80.786 229 40 2 4 230 3 229 1.15e-132 375
MS.gene56672.t1 MTR_3g118010 71.595 257 72 1 32 288 14 269 2.53e-132 376
MS.gene56672.t1 MTR_4g059390 68.015 272 80 3 23 288 7 277 1.69e-130 372
MS.gene56672.t1 MTR_5g010150 83.333 210 33 1 4 213 3 210 5.54e-126 358
MS.gene56672.t1 MTR_4g415300 77.570 214 46 2 1 213 1 213 1.19e-119 342
MS.gene56672.t1 MTR_2g094270 70.213 235 62 2 32 259 17 250 4.40e-119 342
MS.gene56672.t1 MTR_1g095070 77.297 185 41 1 99 283 23 206 5.69e-100 292
MS.gene56672.t1 MTR_4g059390 67.513 197 57 3 23 213 7 202 4.14e-88 261
MS.gene56672.t1 MTR_1g095070 70.718 181 40 2 99 279 23 190 2.89e-85 254
MS.gene56672.t1 MTR_6g069040 39.149 235 127 6 55 285 21 243 9.32e-33 121
MS.gene56672.t1 MTR_1g077540 36.522 230 131 5 55 281 21 238 8.30e-32 119
MS.gene56672.t1 MTR_2g101370 36.364 231 132 5 50 277 15 233 1.19e-31 118
MS.gene56672.t1 MTR_8g013680 36.910 233 130 6 50 277 12 232 8.12e-31 116
MS.gene56672.t1 MTR_5g012810 35.841 226 129 5 55 277 19 231 1.38e-27 107
MS.gene56672.t1 MTR_4g063090 33.333 237 143 5 44 277 10 234 8.55e-27 105
MS.gene56672.t1 MTR_1g115405 33.333 243 135 7 44 277 10 234 1.79e-26 104
MS.gene56672.t1 MTR_7g103030 33.878 245 141 8 45 283 11 240 2.74e-26 103
MS.gene56672.t1 MTR_1g006490 34.091 220 130 6 55 273 19 224 3.14e-25 101
MS.gene56672.t1 MTR_1g006590 35.345 232 119 10 55 277 19 228 4.34e-24 98.2
MS.gene56672.t1 MTR_7g023350 34.052 232 126 7 55 277 21 234 6.34e-24 97.8
MS.gene56672.t1 MTR_5g079570 27.863 262 158 6 25 275 2 243 6.21e-22 92.8
MS.gene56672.t1 MTR_2g060360 31.330 233 130 7 55 277 19 231 3.03e-20 87.8
MS.gene56672.t1 MTR_2g017660 30.093 216 129 6 58 270 42 238 9.54e-20 87.0
MS.gene56672.t1 MTR_4g084880 26.496 234 151 6 58 287 38 254 3.34e-19 85.5
MS.gene56672.t1 MTR_5g063930 30.131 229 143 7 50 277 32 244 2.49e-18 82.8
MS.gene56672.t1 MTR_8g087710 30.126 239 144 8 53 287 37 256 2.56e-18 83.2
MS.gene56672.t1 MTR_2g017590 32.407 216 124 6 58 270 39 235 5.95e-18 82.0
MS.gene56672.t1 MTR_3g008920 27.803 223 142 4 58 277 25 231 3.89e-17 79.7
MS.gene56672.t1 MTR_7g010650 28.139 231 143 7 53 280 35 245 7.64e-17 79.0
MS.gene56672.t1 MTR_4g075570 31.720 186 106 7 102 272 95 274 1.30e-16 79.0
MS.gene56672.t1 MTR_2g017620 31.674 221 129 6 53 270 22 223 1.56e-16 77.8
MS.gene56672.t1 MTR_2g017620 31.674 221 129 6 53 270 36 237 1.87e-16 77.8
MS.gene56672.t1 MTR_8g087720 28.448 232 143 8 52 279 35 247 2.92e-16 77.4
MS.gene56672.t1 MTR_2g017610 32.258 217 123 8 58 270 41 237 4.70e-16 76.6
MS.gene56672.t1 MTR_2g017570 31.019 216 127 7 58 270 41 237 8.40e-16 75.9
MS.gene56672.t1 MTR_4g075590 25.532 235 159 5 47 270 29 258 9.57e-16 76.3
MS.gene56672.t1 MTR_4g006730 26.923 234 143 7 37 270 68 273 3.56e-15 74.7
MS.gene56672.t1 MTR_2g104940 28.638 213 133 7 59 270 84 278 6.10e-14 71.2
MS.gene56672.t1 MTR_4g006630 29.050 179 113 4 92 270 22 186 1.22e-12 66.2
MS.gene56672.t1 MTR_4g006730 29.050 179 113 4 92 270 22 186 1.31e-12 66.2
MS.gene56672.t1 MTR_2g017620 33.529 170 94 6 104 270 3 156 1.42e-12 65.5
MS.gene56672.t1 MTR_1g097840 26.667 225 147 6 59 282 78 285 1.77e-12 67.0
MS.gene56672.t1 MTR_1g070385 26.667 225 146 6 52 275 59 265 2.28e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56672.t1 AT4G00430 86.159 289 37 2 1 289 1 286 0.0 502
MS.gene56672.t1 AT2G45960 84.083 289 42 2 1 289 1 285 1.33e-178 494
MS.gene56672.t1 AT4G23400 85.121 289 40 2 1 289 1 286 4.74e-169 469
MS.gene56672.t1 AT3G61430 84.429 289 41 2 1 289 1 285 6.86e-169 469
MS.gene56672.t1 AT3G61430 84.429 289 41 2 1 289 1 285 6.86e-169 469
MS.gene56672.t1 AT1G01620 84.429 289 41 2 1 289 1 285 6.14e-168 467
MS.gene56672.t1 AT2G45960 83.398 259 39 2 1 259 1 255 1.12e-156 438
MS.gene56672.t1 AT2G45960 83.398 259 39 2 1 259 1 255 1.72e-155 436
MS.gene56672.t1 AT2G37180 71.863 263 67 3 32 288 14 275 6.65e-134 380
MS.gene56672.t1 AT2G37170 71.863 263 67 3 32 288 57 318 4.35e-133 380
MS.gene56672.t1 AT4G35100 71.984 257 71 1 32 288 15 270 1.08e-132 377
MS.gene56672.t1 AT4G35100 71.984 257 71 1 32 288 15 270 1.08e-132 377
MS.gene56672.t1 AT2G37170 71.863 263 67 3 32 288 14 275 1.25e-132 377
MS.gene56672.t1 AT3G53420 72.453 265 62 4 32 288 16 277 1.28e-132 377
MS.gene56672.t1 AT3G53420 72.453 265 62 4 32 288 16 277 1.28e-132 377
MS.gene56672.t1 AT2G16850 71.595 257 72 1 32 288 13 268 6.46e-131 373
MS.gene56672.t1 AT1G01620 86.916 214 27 1 76 289 1 213 1.67e-130 369
MS.gene56672.t1 AT3G54820 69.434 265 70 3 32 288 15 276 7.21e-130 370
MS.gene56672.t1 AT4G00430 82.949 217 34 2 1 217 1 214 6.82e-128 363
MS.gene56672.t1 AT5G60660 68.302 265 73 4 32 288 16 277 2.33e-126 362
MS.gene56672.t1 AT2G39010 67.300 263 79 2 32 288 15 276 5.76e-126 360
MS.gene56672.t1 AT2G39010 65.426 188 58 2 32 213 15 201 5.25e-82 246
MS.gene56672.t1 AT3G16240 36.726 226 128 5 55 277 19 232 5.32e-31 116
MS.gene56672.t1 AT5G47450 37.662 231 129 7 50 277 14 232 1.25e-30 115
MS.gene56672.t1 AT4G01470 35.443 237 138 5 44 277 10 234 2.75e-30 114
MS.gene56672.t1 AT1G73190 35.391 243 130 7 55 285 24 251 6.36e-30 114
MS.gene56672.t1 AT1G17810 34.728 239 135 7 55 284 24 250 2.40e-29 112
MS.gene56672.t1 AT4G17340 36.283 226 129 6 55 277 19 232 4.32e-27 106
MS.gene56672.t1 AT2G36830 32.669 251 130 8 44 279 10 236 4.48e-26 103
MS.gene56672.t1 AT3G26520 32.751 229 133 6 55 277 22 235 2.90e-25 101
MS.gene56672.t1 AT2G25810 35.652 230 130 7 55 281 19 233 4.18e-24 98.2
MS.gene56672.t1 AT3G47440 31.687 243 135 8 50 281 18 240 9.98e-23 94.7
MS.gene56672.t1 AT1G31885 29.150 247 154 6 26 270 22 249 5.23e-20 88.6
MS.gene56672.t1 AT4G19030 27.985 268 175 7 26 288 26 280 5.31e-19 85.5
MS.gene56672.t1 AT1G31885 29.082 196 129 4 77 270 8 195 4.91e-16 76.6
MS.gene56672.t1 AT4G18910 27.509 269 175 8 26 287 21 276 5.08e-16 77.0
MS.gene56672.t1 AT5G37820 28.571 217 129 7 58 269 82 277 6.04e-16 77.0
MS.gene56672.t1 AT5G37820 28.571 217 129 7 58 269 46 241 6.13e-16 76.6
MS.gene56672.t1 AT2G34390 26.429 280 177 9 6 277 2 260 3.39e-15 74.7
MS.gene56672.t1 AT4G10380 24.901 253 158 6 33 270 41 276 2.10e-12 66.6
MS.gene56672.t1 AT1G80760 25.301 249 166 6 32 280 60 288 3.22e-12 66.2
MS.gene56672.t1 AT5G37810 26.728 217 133 7 58 269 46 241 4.24e-12 65.5
MS.gene56672.t1 AT2G34390 26.667 225 145 7 58 277 9 218 6.40e-12 64.7

Find 81 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCCAAGTGGTTGTCTTTC+TGG 0.098743 5.1:+64280082 None:intergenic
CAAGGAATTGCTTGGGCTTT+TGG 0.147648 5.1:-64279857 MS.gene56672:CDS
TATTCCTTCAGTGGATCTTC+TGG 0.159475 5.1:-64278214 MS.gene56672:intron
TTAATACCAGTTCCAGTAAT+TGG 0.193062 5.1:+64278407 None:intergenic
TTCTGGGTGGGACCATTTAT+TGG 0.238352 5.1:-64278197 MS.gene56672:CDS
TTTGAAGGAAAGACAAGATT+TGG 0.252728 5.1:-64279101 MS.gene56672:CDS
CGCTGTGTTCTTGGTTCACT+TGG 0.261822 5.1:-64278438 MS.gene56672:CDS
GCAAGAGCTGCTCCAATAAA+TGG 0.306781 5.1:+64278185 None:intergenic
AATTGGGTTCGCTGTGTTCT+TGG 0.323972 5.1:-64278447 MS.gene56672:CDS
AAACAGTGATATAAAGAAAA+AGG 0.328096 5.1:+64279933 None:intergenic
ACAAGATTTGGAGATCTAAA+AGG 0.340417 5.1:-64279089 MS.gene56672:CDS
GGTGGCTCTTGATAGTCCTT+AGG 0.343217 5.1:+64280037 None:intergenic
TCCCAAGTGGTTGTCTTTCT+GGG 0.348961 5.1:+64280083 None:intergenic
CTACATGGTGATGCAATGTT+TGG 0.359716 5.1:-64279156 MS.gene56672:CDS
AAAGAACAGGATGTGTCTTT+AGG 0.370646 5.1:-64280118 MS.gene56672:CDS
ATTCCTTCAGTGGATCTTCT+GGG 0.373293 5.1:-64278213 MS.gene56672:intron
GATAGTCCTTAGGGTCATCT+TGG 0.381110 5.1:+64280047 None:intergenic
TAAATCCAGCAGTGACATTT+GGG 0.385302 5.1:-64279220 MS.gene56672:CDS
ATAAATCCAGCAGTGACATT+TGG 0.387473 5.1:-64279221 MS.gene56672:CDS
TACTGTACTGCTGGAATCTC+AGG 0.389203 5.1:-64279812 MS.gene56672:intron
TTGGGTGCTATTTGTGGTGC+TGG 0.389900 5.1:-64279137 MS.gene56672:CDS
TTGTCTTTCTGGGAATTTGT+TGG 0.391781 5.1:+64280093 None:intergenic
TTGGCCACTATCCCAATTAC+TGG 0.392883 5.1:-64278419 MS.gene56672:CDS
GCTCTTGTTTACTGTACTGC+TGG 0.392947 5.1:-64279821 MS.gene56672:CDS
TGGGGATTCAAGGAATTGCT+TGG 0.394052 5.1:-64279865 MS.gene56672:CDS
TCGTGGTCTTTCTACAGAGC+TGG 0.396210 5.1:-64279980 MS.gene56672:CDS
ATTTGATCACTTTGACTTGA+AGG 0.406054 5.1:+64278127 None:intergenic
CAATGTTTGGGTGCTATTTG+TGG 0.409953 5.1:-64279143 MS.gene56672:CDS
TAATACCAGTTCCAGTAATT+GGG 0.410603 5.1:+64278408 None:intergenic
GGGGATTCAAGGAATTGCTT+GGG 0.411864 5.1:-64279864 MS.gene56672:CDS
TGTAAGTCTGTGGGGATTCA+AGG 0.414562 5.1:-64279875 MS.gene56672:CDS
ACTATCCCAATTACTGGAAC+TGG 0.426227 5.1:-64278413 MS.gene56672:CDS
GGTGCCAACTTTGTTGCTCC+TGG 0.427799 5.1:-64279065 MS.gene56672:CDS
AGCACCAAGACCATCACCTT+TGG 0.435976 5.1:+64279037 None:intergenic
GTGGCTCTTGATAGTCCTTA+GGG 0.463360 5.1:+64280038 None:intergenic
TACATGGTGATGCAATGTTT+GGG 0.464448 5.1:-64279155 MS.gene56672:CDS
ATTAACCCTGCTCGTAGTCT+TGG 0.478309 5.1:-64278389 MS.gene56672:CDS
TGTGGTGCTGGTGTTGTGAA+AGG 0.479519 5.1:-64279125 MS.gene56672:CDS
TCAAACAACGGTGCTGGTGG+TGG 0.481920 5.1:+64280019 None:intergenic
AATAGCCCAAATGTCACTGC+TGG 0.486149 5.1:+64279215 None:intergenic
TCTTCAACAAGGACCTTGGT+TGG 0.486748 5.1:-64278355 MS.gene56672:CDS
GGTCTTGGTGCTGAAATTGT+TGG 0.490732 5.1:-64279026 MS.gene56672:CDS
TACGGGAACATGAGAGTCTC+TGG 0.492319 5.1:+64278950 None:intergenic
AAGAACACAGCGAACCCAAT+TGG 0.494251 5.1:+64278449 None:intergenic
GATGGCTCAAACAACGGTGC+TGG 0.510215 5.1:+64280013 None:intergenic
TCACTTTGACTTGAAGGGAA+TGG 0.512515 5.1:+64278133 None:intergenic
AGCCATCTGAACTGACTTCG+TGG 0.512898 5.1:-64279997 MS.gene56672:CDS
AGTTCCAGTAATTGGGATAG+TGG 0.513529 5.1:+64278415 None:intergenic
TTTGATCACTTTGACTTGAA+GGG 0.520518 5.1:+64278128 None:intergenic
AGTTCAGATGGCTCAAACAA+CGG 0.521397 5.1:+64280007 None:intergenic
ATTATCTTCAACAAGGACCT+TGG 0.525491 5.1:-64278359 MS.gene56672:CDS
CTTCAGTGGATCTTCTGGGT+GGG 0.526287 5.1:-64278209 MS.gene56672:intron
CGTGGTCTTTCTACAGAGCT+GGG 0.526726 5.1:-64279979 MS.gene56672:CDS
GTTGCTCCTGGTTACACCAA+AGG 0.542015 5.1:-64279053 MS.gene56672:CDS
TACACCAAAGGTGATGGTCT+TGG 0.554514 5.1:-64279041 MS.gene56672:CDS
GATTCTAAGTGTAAGTCTGT+GGG 0.554986 5.1:-64279884 MS.gene56672:CDS
GACAAGAGCATTGTTCTACA+TGG 0.557563 5.1:-64279171 MS.gene56672:CDS
GACCACGAAGTCAGTTCAGA+TGG 0.563006 5.1:+64279995 None:intergenic
CCTGGTTACACCAAAGGTGA+TGG 0.563447 5.1:-64279047 MS.gene56672:CDS
CCATCACCTTTGGTGTAACC+AGG 0.566415 5.1:+64279047 None:intergenic
AGCATCGGTGGCTGAGAAGA+CGG 0.570780 5.1:+64278983 None:intergenic
GGCGCTACGTTTAGCATCGG+TGG 0.574723 5.1:+64278971 None:intergenic
GCAGCACCAAGACTACGAGC+AGG 0.585086 5.1:+64278383 None:intergenic
TCAAAGCCAAGATGACCCTA+AGG 0.586870 5.1:-64280053 MS.gene56672:CDS
TGATTCTAAGTGTAAGTCTG+TGG 0.589733 5.1:-64279885 MS.gene56672:CDS
CCTTCAGTGGATCTTCTGGG+TGG 0.594060 5.1:-64278210 MS.gene56672:intron
TCCCAGAAAGACAACCACTT+GGG 0.597140 5.1:-64280084 MS.gene56672:CDS
GTAGAACAATGCTCTTGTCA+AGG 0.610941 5.1:+64279175 None:intergenic
CTTTGAGCTGCAATCCCAAG+TGG 0.615951 5.1:+64280070 None:intergenic
TTCCCAGAAAGACAACCACT+TGG 0.616137 5.1:-64280085 MS.gene56672:CDS
GGCTCAAACAACGGTGCTGG+TGG 0.625378 5.1:+64280016 None:intergenic
GTAACCAGGAGCAACAAAGT+TGG 0.636963 5.1:+64279061 None:intergenic
ACAGCGAACCCAATTGGCAA+TGG 0.642498 5.1:+64278455 None:intergenic
TGCTGCTATTATCTTCAACA+AGG 0.649253 5.1:-64278366 MS.gene56672:CDS
ATTCTAAGTGTAAGTCTGTG+GGG 0.650800 5.1:-64279883 MS.gene56672:CDS
TCTGGCGCTACGTTTAGCAT+CGG 0.662595 5.1:+64278968 None:intergenic
CAGCACCAAGACTACGAGCA+GGG 0.662928 5.1:+64278384 None:intergenic
AGATTTGGAGATCTAAAAGG+TGG 0.683343 5.1:-64279086 MS.gene56672:CDS
TTACATGATCATTCCAACCA+AGG 0.692776 5.1:+64278342 None:intergenic
TGGCTCTGATTACGACCACG+TGG 0.762119 5.1:+64278153 None:intergenic
TCTTGCAGCACTCTACCACG+TGG 0.776974 5.1:-64278168 MS.gene56672:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTTATCAAATTATCA+TGG + chr5.1:64278887-64278906 None:intergenic 10.0%
!!! TATTCTTTTAATTTAACTTT+AGG + chr5.1:64278799-64278818 None:intergenic 10.0%
!!! TATTTTTATTTTTCTGTTAT+AGG - chr5.1:64278581-64278600 MS.gene56672:intron 10.0%
!! AATATAACTAATTATTTGTG+TGG - chr5.1:64279757-64279776 MS.gene56672:intron 15.0%
!! ACTTAATAACTAATTATTAC+AGG - chr5.1:64279731-64279750 MS.gene56672:intron 15.0%
!! ATGTATGTTATTATTATTCA+GGG + chr5.1:64278494-64278513 None:intergenic 15.0%
!!! AATAAATAAGGGTATTATAA+TGG + chr5.1:64279482-64279501 None:intergenic 15.0%
!!! ATTTTGAGAAAAAGAAAAAT+GGG + chr5.1:64279416-64279435 None:intergenic 15.0%
!!! TATTTTGAGAAAAAGAAAAA+TGG + chr5.1:64279417-64279436 None:intergenic 15.0%
!! AAAAATAATAAATGCTGCAT+TGG + chr5.1:64279457-64279476 None:intergenic 20.0%
!! AAACAGTGATATAAAGAAAA+AGG + chr5.1:64278330-64278349 None:intergenic 20.0%
!! AGTTATTAAGTGTATATTGT+CGG + chr5.1:64279722-64279741 None:intergenic 20.0%
!! ATTAATTATTATTCCTTCAG+TGG - chr5.1:64280037-64280056 MS.gene56672:CDS 20.0%
!! CATGTATGTTATTATTATTC+AGG + chr5.1:64278495-64278514 None:intergenic 20.0%
!! GAGTATTTATAGTAATTTGA+TGG + chr5.1:64279365-64279384 None:intergenic 20.0%
!! TACATAAATTTCATTCAAAC+TGG + chr5.1:64278630-64278649 None:intergenic 20.0%
!!! ATTAATTGTGTTGATTTTGA+TGG + chr5.1:64279577-64279596 None:intergenic 20.0%
!!! CTTATTTTATGAGTAGATTT+TGG - chr5.1:64279780-64279799 MS.gene56672:intron 20.0%
! AAACGACTATTATTTAGAGA+CGG - chr5.1:64279626-64279645 MS.gene56672:intron 25.0%
! AGATTGATGCACAATTATTA+AGG + chr5.1:64279605-64279624 None:intergenic 25.0%
! CCACTAACTACAATTAATAA+GGG - chr5.1:64279525-64279544 MS.gene56672:intron 25.0%
! GGATGAAGTTCAATAAATAA+GGG + chr5.1:64279493-64279512 None:intergenic 25.0%
! TCAGATACTGGATATATAAT+TGG - chr5.1:64279686-64279705 MS.gene56672:intron 25.0%
! TCCACTAACTACAATTAATA+AGG - chr5.1:64279524-64279543 MS.gene56672:intron 25.0%
! TGGATGAAGTTCAATAAATA+AGG + chr5.1:64279494-64279513 None:intergenic 25.0%
!!! CTATTTTTATAGTCAGATAC+TGG - chr5.1:64279674-64279693 MS.gene56672:intron 25.0%
AAGAGACAATTATTGTGAGA+CGG + chr5.1:64279391-64279410 None:intergenic 30.0%
AAGTAACAACTACCATGATA+AGG - chr5.1:64278855-64278874 MS.gene56672:intron 30.0%
ACAAGATTTGGAGATCTAAA+AGG - chr5.1:64279171-64279190 MS.gene56672:CDS 30.0%
CCCTTATTAATTGTAGTTAG+TGG + chr5.1:64279528-64279547 None:intergenic 30.0%
GACTAAATTAGTTGTTCCTA+TGG - chr5.1:64278603-64278622 MS.gene56672:intron 30.0%
TAATACCAGTTCCAGTAATT+GGG + chr5.1:64279855-64279874 None:intergenic 30.0%
TGATGGTTATGATTGATTGA+CGG + chr5.1:64279348-64279367 None:intergenic 30.0%
TTAATACCAGTTCCAGTAAT+TGG + chr5.1:64279856-64279875 None:intergenic 30.0%
TTTGAAGGAAAGACAAGATT+TGG - chr5.1:64279159-64279178 MS.gene56672:CDS 30.0%
! GTATCGATCTAATGTTTGTT+TGG - chr5.1:64278949-64278968 MS.gene56672:intron 30.0%
!!! ATCACTGTTTTGACTGTTAT+GGG - chr5.1:64278340-64278359 MS.gene56672:intron 30.0%
!!! TATCACTGTTTTGACTGTTA+TGG - chr5.1:64278339-64278358 MS.gene56672:intron 30.0%
AAAGAACAGGATGTGTCTTT+AGG - chr5.1:64278142-64278161 MS.gene56672:CDS 35.0%
AGATTTGGAGATCTAAAAGG+TGG - chr5.1:64279174-64279193 MS.gene56672:CDS 35.0%
AGTTAGTGGAGTAGTTAAGT+TGG + chr5.1:64279514-64279533 None:intergenic 35.0%
ATAAATCCAGCAGTGACATT+TGG - chr5.1:64279039-64279058 MS.gene56672:CDS 35.0%
ATTATCTTCAACAAGGACCT+TGG - chr5.1:64279901-64279920 MS.gene56672:CDS 35.0%
ATTCTAAGTGTAAGTCTGTG+GGG - chr5.1:64278377-64278396 MS.gene56672:CDS 35.0%
GACTATTATTTAGAGACGGA+GGG - chr5.1:64279630-64279649 MS.gene56672:intron 35.0%
GATTCTAAGTGTAAGTCTGT+GGG - chr5.1:64278376-64278395 MS.gene56672:CDS 35.0%
TAAATCCAGCAGTGACATTT+GGG - chr5.1:64279040-64279059 MS.gene56672:CDS 35.0%
TCATGGATATGTCCTTATCA+TGG + chr5.1:64278870-64278889 None:intergenic 35.0%
TGATTCTAAGTGTAAGTCTG+TGG - chr5.1:64278375-64278394 MS.gene56672:CDS 35.0%
TGCTGCTATTATCTTCAACA+AGG - chr5.1:64279894-64279913 MS.gene56672:CDS 35.0%
TTACATGATCATTCCAACCA+AGG + chr5.1:64279921-64279940 None:intergenic 35.0%
TTTCATTCAAACTGGTCCAT+AGG + chr5.1:64278622-64278641 None:intergenic 35.0%
! TACATGGTGATGCAATGTTT+GGG - chr5.1:64279105-64279124 MS.gene56672:CDS 35.0%
! TTGTCTTTCTGGGAATTTGT+TGG + chr5.1:64278170-64278189 None:intergenic 35.0%
!! AGTGACATTTGGGCTATTTT+TGG - chr5.1:64279050-64279069 MS.gene56672:CDS 35.0%
AATTATGTACCGTGTGTTGC+AGG - chr5.1:64279009-64279028 MS.gene56672:CDS 40.0%
ACTATCCCAATTACTGGAAC+TGG - chr5.1:64279847-64279866 MS.gene56672:CDS 40.0%
AGACAATTATTGTGAGACGG+AGG + chr5.1:64279388-64279407 None:intergenic 40.0%
AGTTCAGATGGCTCAAACAA+CGG + chr5.1:64278256-64278275 None:intergenic 40.0%
AGTTCCAGTAATTGGGATAG+TGG + chr5.1:64279848-64279867 None:intergenic 40.0%
ATTATGTACCGTGTGTTGCA+GGG - chr5.1:64279010-64279029 MS.gene56672:CDS 40.0%
ATTCCTTCAGTGGATCTTCT+GGG - chr5.1:64280047-64280066 MS.gene56672:CDS 40.0%
CGACTATTATTTAGAGACGG+AGG - chr5.1:64279629-64279648 MS.gene56672:intron 40.0%
GACAATTATTGTGAGACGGA+GGG + chr5.1:64279387-64279406 None:intergenic 40.0%
GTAGAACAATGCTCTTGTCA+AGG + chr5.1:64279088-64279107 None:intergenic 40.0%
TATTCCTTCAGTGGATCTTC+TGG - chr5.1:64280046-64280065 MS.gene56672:CDS 40.0%
TGACGGTGTAAAGAGACTTA+CGG + chr5.1:64279331-64279350 None:intergenic 40.0%
! CTACATGGTGATGCAATGTT+TGG - chr5.1:64279104-64279123 MS.gene56672:CDS 40.0%
! TTTTGGCACCATTGCCAATT+GGG - chr5.1:64279797-64279816 MS.gene56672:intron 40.0%
!! ATTTTGGCACCATTGCCAAT+TGG - chr5.1:64279796-64279815 MS.gene56672:intron 40.0%
!! CAATGTTTGGGTGCTATTTG+TGG - chr5.1:64279117-64279136 MS.gene56672:CDS 40.0%
!! GACAAGAGCATTGTTCTACA+TGG - chr5.1:64279089-64279108 MS.gene56672:CDS 40.0%
!! GGTGTTGTGAAAGGTTTTGA+AGG - chr5.1:64279144-64279163 MS.gene56672:CDS 40.0%
!! TCACTTTGACTTGAAGGGAA+TGG + chr5.1:64280130-64280149 None:intergenic 40.0%
AAGAACACAGCGAACCCAAT+TGG + chr5.1:64279814-64279833 None:intergenic 45.0%
AATAGCCCAAATGTCACTGC+TGG + chr5.1:64279048-64279067 None:intergenic 45.0%
AATTGGGTTCGCTGTGTTCT+TGG - chr5.1:64279813-64279832 MS.gene56672:CDS 45.0%
ATCCCAAGTGGTTGTCTTTC+TGG + chr5.1:64278181-64278200 None:intergenic 45.0%
ATTAACCCTGCTCGTAGTCT+TGG - chr5.1:64279871-64279890 MS.gene56672:CDS 45.0%
GACGGTGTAAAGAGACTTAC+GGG + chr5.1:64279330-64279349 None:intergenic 45.0%
GATAGTCCTTAGGGTCATCT+TGG + chr5.1:64278216-64278235 None:intergenic 45.0%
GCAAGAGCTGCTCCAATAAA+TGG + chr5.1:64280078-64280097 None:intergenic 45.0%
GGGGATTCAAGGAATTGCTT+GGG - chr5.1:64278396-64278415 MS.gene56672:CDS 45.0%
GTAACCAGGAGCAACAAAGT+TGG + chr5.1:64279202-64279221 None:intergenic 45.0%
TACTGTACTGCTGGAATCTC+AGG - chr5.1:64278448-64278467 MS.gene56672:CDS 45.0%
TCAAAGCCAAGATGACCCTA+AGG - chr5.1:64278207-64278226 MS.gene56672:intron 45.0%
TCCCAAGTGGTTGTCTTTCT+GGG + chr5.1:64278180-64278199 None:intergenic 45.0%
TCCCAGAAAGACAACCACTT+GGG - chr5.1:64278176-64278195 MS.gene56672:CDS 45.0%
TCTTCAACAAGGACCTTGGT+TGG - chr5.1:64279905-64279924 MS.gene56672:CDS 45.0%
TGGGGATTCAAGGAATTGCT+TGG - chr5.1:64278395-64278414 MS.gene56672:CDS 45.0%
TGTAAGTCTGTGGGGATTCA+AGG - chr5.1:64278385-64278404 MS.gene56672:CDS 45.0%
TTATGTACCGTGTGTTGCAG+GGG - chr5.1:64279011-64279030 MS.gene56672:CDS 45.0%
TTCCCAGAAAGACAACCACT+TGG - chr5.1:64278175-64278194 MS.gene56672:CDS 45.0%
TTCTGGGTGGGACCATTTAT+TGG - chr5.1:64280063-64280082 MS.gene56672:CDS 45.0%
TTGGCCACTATCCCAATTAC+TGG - chr5.1:64279841-64279860 MS.gene56672:CDS 45.0%
! CAAGGAATTGCTTGGGCTTT+TGG - chr5.1:64278403-64278422 MS.gene56672:CDS 45.0%
! GCTCTTGTTTACTGTACTGC+TGG - chr5.1:64278439-64278458 MS.gene56672:CDS 45.0%
! GTGGCTCTTGATAGTCCTTA+GGG + chr5.1:64278225-64278244 None:intergenic 45.0%
! TACACCAAAGGTGATGGTCT+TGG - chr5.1:64279219-64279238 MS.gene56672:CDS 45.0%
!! GGTCTTGGTGCTGAAATTGT+TGG - chr5.1:64279234-64279253 MS.gene56672:intron 45.0%
!!! CATTTGGGCTATTTTTGGCG+AGG - chr5.1:64279055-64279074 MS.gene56672:CDS 45.0%
ACAGCGAACCCAATTGGCAA+TGG + chr5.1:64279808-64279827 None:intergenic 50.0%
AGCACCAAGACCATCACCTT+TGG + chr5.1:64279226-64279245 None:intergenic 50.0%
CCTGGTTACACCAAAGGTGA+TGG - chr5.1:64279213-64279232 MS.gene56672:CDS 50.0%
CGCTGTGTTCTTGGTTCACT+TGG - chr5.1:64279822-64279841 MS.gene56672:CDS 50.0%
CGTGGTCTTTCTACAGAGCT+GGG - chr5.1:64278281-64278300 MS.gene56672:intron 50.0%
CTTCAGTGGATCTTCTGGGT+GGG - chr5.1:64280051-64280070 MS.gene56672:CDS 50.0%
CTTTGAGCTGCAATCCCAAG+TGG + chr5.1:64278193-64278212 None:intergenic 50.0%
GACCACGAAGTCAGTTCAGA+TGG + chr5.1:64278268-64278287 None:intergenic 50.0%
GTTGCTCCTGGTTACACCAA+AGG - chr5.1:64279207-64279226 MS.gene56672:CDS 50.0%
TACGGGAACATGAGAGTCTC+TGG + chr5.1:64279313-64279332 None:intergenic 50.0%
TATGTACCGTGTGTTGCAGG+GGG - chr5.1:64279012-64279031 MS.gene56672:CDS 50.0%
TCGTGGTCTTTCTACAGAGC+TGG - chr5.1:64278280-64278299 MS.gene56672:intron 50.0%
TCTGGCGCTACGTTTAGCAT+CGG + chr5.1:64279295-64279314 None:intergenic 50.0%
! AGCCATCTGAACTGACTTCG+TGG - chr5.1:64278263-64278282 MS.gene56672:intron 50.0%
! CCATCACCTTTGGTGTAACC+AGG + chr5.1:64279216-64279235 None:intergenic 50.0%
! GGAATTGCTTGGGCTTTTGG+TGG - chr5.1:64278406-64278425 MS.gene56672:CDS 50.0%
! GGTGGCTCTTGATAGTCCTT+AGG + chr5.1:64278226-64278245 None:intergenic 50.0%
!! TGTGGTGCTGGTGTTGTGAA+AGG - chr5.1:64279135-64279154 MS.gene56672:CDS 50.0%
!! TTGGGTGCTATTTGTGGTGC+TGG - chr5.1:64279123-64279142 MS.gene56672:CDS 50.0%
ATGTGACCCCCTGCAACACA+CGG + chr5.1:64279021-64279040 None:intergenic 55.0%
CAGCACCAAGACTACGAGCA+GGG + chr5.1:64279879-64279898 None:intergenic 55.0%
CCACCCAGAAGATCCACTGA+AGG + chr5.1:64280053-64280072 None:intergenic 55.0%
CCTTCAGTGGATCTTCTGGG+TGG - chr5.1:64280050-64280069 MS.gene56672:CDS 55.0%
GATGGCTCAAACAACGGTGC+TGG + chr5.1:64278250-64278269 None:intergenic 55.0%
GGTGCCAACTTTGTTGCTCC+TGG - chr5.1:64279195-64279214 MS.gene56672:CDS 55.0%
TCTTGCAGCACTCTACCACG+TGG - chr5.1:64280092-64280111 MS.gene56672:CDS 55.0%
!! AGCATCGGTGGCTGAGAAGA+CGG + chr5.1:64279280-64279299 None:intergenic 55.0%
!! TCAAACAACGGTGCTGGTGG+TGG + chr5.1:64278244-64278263 None:intergenic 55.0%
!! TGGCTCTGATTACGACCACG+TGG + chr5.1:64280110-64280129 None:intergenic 55.0%
GCAGCACCAAGACTACGAGC+AGG + chr5.1:64279880-64279899 None:intergenic 60.0%
! GGCGCTACGTTTAGCATCGG+TGG + chr5.1:64279292-64279311 None:intergenic 60.0%
! GGCTCAAACAACGGTGCTGG+TGG + chr5.1:64278247-64278266 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 64278133 64280149 64278133 ID=MS.gene56672
chr5.1 mRNA 64278133 64280149 64278133 ID=MS.gene56672.t1;Parent=MS.gene56672
chr5.1 exon 64279813 64280149 64279813 ID=MS.gene56672.t1.exon1;Parent=MS.gene56672.t1
chr5.1 CDS 64279813 64280149 64279813 ID=cds.MS.gene56672.t1;Parent=MS.gene56672.t1
chr5.1 exon 64278953 64279251 64278953 ID=MS.gene56672.t1.exon2;Parent=MS.gene56672.t1
chr5.1 CDS 64278953 64279251 64278953 ID=cds.MS.gene56672.t1;Parent=MS.gene56672.t1
chr5.1 exon 64278346 64278486 64278346 ID=MS.gene56672.t1.exon3;Parent=MS.gene56672.t1
chr5.1 CDS 64278346 64278486 64278346 ID=cds.MS.gene56672.t1;Parent=MS.gene56672.t1
chr5.1 exon 64278133 64278225 64278133 ID=MS.gene56672.t1.exon4;Parent=MS.gene56672.t1
chr5.1 CDS 64278133 64278225 64278133 ID=cds.MS.gene56672.t1;Parent=MS.gene56672.t1
Gene Sequence

>MS.gene56672

ATGGAAGCTAAAGAACAGGATGTGTCTTTAGGAGCCAACAAATTCCCAGAAAGACAACCACTTGGGATTGCAGCTCAAAGCCAAGATGACCCTAAGGACTATCAAGAGCCACCACCAGCACCGTTGTTTGAGCCATCTGAACTGACTTCGTGGTCTTTCTACAGAGCTGGGATAGCTGAGTTCGTTGCTACTTTCCTTTTTCTTTATATCACTGTTTTGACTGTTATGGGTGTTGTTAGAGCTGATTCTAAGTGTAAGTCTGTGGGGATTCAAGGAATTGCTTGGGCTTTTGGTGGCATGATCTTTGCTCTTGTTTACTGTACTGCTGGAATCTCAGGTATAGAATATTTTCTATTTTCCCTGAATAATAATAACATACATGTAATACATATTCTTTAGTACTTATAATTTTTGAAAGTTAGTTCATTCATGAATTTTGTTCAATTTTTATTTTTATTTTTCTGTTATAGGACTAAATTAGTTGTTCCTATGGACCAGTTTGAATGAAATTTATGTAAAGAATTAATTTAAGAAAGTGTAATTTTATAGAGATTAAAAACATATTTAACTCTAATAATTCTTTAAAAAAAACTAATAATAATAACGATAATAATAATTTCTGAATATATATTAGCAGTTTTTTGTGAAATTAAGATAAGCTCACCTAAAGTTAAATTAAAAGAATAACATAAACTATGTGCTAGTGACAGTGTGACACTAACAAGTAACAACTACCATGATAAGGACATATCCATGATAATTTGATAAAATATTTCAACAAAAATAAATAATTTGATAAAACAAAAGTAATTAAATGTATCGATCTAATGTTTGTTTGGTCTTTGAATCAATTTTTTATTAGTCAATTTGATCTAAAATTATGTACCGTGTGTTGCAGGGGGTCACATAAATCCAGCAGTGACATTTGGGCTATTTTTGGCGAGGAAATTATCCTTGACAAGAGCATTGTTCTACATGGTGATGCAATGTTTGGGTGCTATTTGTGGTGCTGGTGTTGTGAAAGGTTTTGAAGGAAAGACAAGATTTGGAGATCTAAAAGGTGGTGCCAACTTTGTTGCTCCTGGTTACACCAAAGGTGATGGTCTTGGTGCTGAAATTGTTGGCACTTTCATTCTTGTTTACACCGTCTTCTCAGCCACCGATGCTAAACGTAGCGCCAGAGACTCTCATGTTCCCGTAAGTCTCTTTACACCGTCAATCAATCATAACCATCAAATTACTATAAATACTCCCTCCGTCTCACAATAATTGTCTCTTTACCCATTTTTCTTTTTCTCAAAATAATTGTCACTTTAGAATACCAATGCAGCATTTATTATTTTTTTCCATTATAATACCCTTATTTATTGAACTTCATCCAACTTAACTACTCCACTAACTACAATTAATAAGGGTGTTTTAGTAAATGATACTAATTTTACCATCAAAATCAACACAATTAATCATTTCCTTAATAATTGTGCATCAATCTTAAACGACTATTATTTAGAGACGGAGGGAGTATTTGACTTTAGTCGTGACTATTTTTATAGTCAGATACTGGATATATAATTGGTTGTGATTTACCGACAATATACACTTAATAACTAATTATTACAGGAATAATATAACTAATTATTTGTGTGGCTTATTTTATGAGTAGATTTTGGCACCATTGCCAATTGGGTTCGCTGTGTTCTTGGTTCACTTGGCCACTATCCCAATTACTGGAACTGGTATTAACCCTGCTCGTAGTCTTGGTGCTGCTATTATCTTCAACAAGGACCTTGGTTGGAATGATCATGTAAGTTGAAAAATTGAAATTAGTTATAAATTCGTATTGTTTTTGTAAAATTATATATTTATTTTTCTGAATTTTCTTATCTCATTTATCTTTTGTGTTCATTAATTATTATTCCTTCAGTGGATCTTCTGGGTGGGACCATTTATTGGAGCAGCTCTTGCAGCACTCTACCACGTGGTCGTAATCAGAGCCATTCCCTTCAAGTCAAAGTGA
Protein sequence

>MS.gene56672.t1

MEAKEQDVSLGANKFPERQPLGIAAQSQDDPKDYQEPPPAPLFEPSELTSWSFYRAGIAEFVATFLFLYITVLTVMGVVRADSKCKSVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALFYMVMQCLGAICGAGVVKGFEGKTRFGDLKGGANFVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRSARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIFNKDLGWNDHWIFWVGPFIGAALAALYHVVVIRAIPFKSK