AlfalfaGEDB | MS.gene052015

M. sativa cultivar XinJiangDaYe / MS.gene052015

KEGG: K14004
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00400.35 PF12894.10

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052015.t1	PNX74154.1	98.7	301	4	0	1	301	1	301	3.30E-136	494.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052015.t1	O64740	79.1	302	62	1	1	301	1	302	6.8e-98	358.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052015.t1	A0A2K3L6J4	98.7	301	4	0	1	301	1	301	2.4e-136	494.6

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene052015	-0.812587	3.93E-51	-1.69E-46
MS.gene049222	MS.gene052015	-0.800939	1.14E-48	-1.69E-46
MS.gene050479	MS.gene052015	0.811986	5.32E-51	-1.69E-46
MS.gene050504	MS.gene052015	-0.812776	3.58E-51	-1.69E-46
MS.gene050547	MS.gene052015	0.815587	8.57E-52	-1.69E-46
MS.gene050577	MS.gene052015	-0.804202	2.42E-49	-1.69E-46
MS.gene050699	MS.gene052015	-0.800342	1.51E-48	-1.69E-46
MS.gene050937	MS.gene052015	0.851258	9.98E-61	-1.69E-46
MS.gene051973	MS.gene052015	0.821668	3.57E-53	-1.69E-46
MS.gene052015	MS.gene052494	0.828856	7.12E-55	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene030377	MS.gene052015	PPI
MS.gene032173	MS.gene052015	PPI
MS.gene047763	MS.gene052015	PPI
MS.gene052015	MS.gene010482	PPI
MS.gene052015	MS.gene73118	PPI
MS.gene052015	MS.gene70974	PPI
MS.gene052015	MS.gene98722	PPI
MS.gene052015	MS.gene59349	PPI
MS.gene052015	MS.gene72123	PPI
MS.gene052015	MS.gene048821	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052015.t1	MTR_3g054090	98.339	301	5	0	1	301	1	301	0.0	603
MS.gene052015.t1	MTR_6g072020	26.433	314	194	9	20	301	18	326	1.43e-17	82.0
MS.gene052015.t1	MTR_7g104550	25.397	252	155	10	46	286	7	236	1.63e-12	67.4
MS.gene052015.t1	MTR_5g016370	24.590	305	155	13	23	284	318	590	2.87e-12	67.4
MS.gene052015.t1	MTR_4g095058	27.660	235	134	10	54	284	371	573	5.89e-12	66.2
MS.gene052015.t1	MTR_4g095058	27.660	235	134	10	54	284	369	571	6.21e-12	66.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052015.t1	AT2G30050	84.768	302	45	1	1	301	1	302	0.0	531
MS.gene052015.t1	AT3G01340	82.119	302	53	1	1	301	1	302	0.0	521
MS.gene052015.t1	AT3G01340	82.119	302	53	1	1	301	1	302	0.0	521
MS.gene052015.t1	AT4G02730	27.165	254	149	11	46	298	34	252	1.03e-12	68.2
MS.gene052015.t1	AT1G64350	25.180	278	167	11	55	301	56	323	2.71e-12	66.6
MS.gene052015.t1	AT2G26060	27.490	251	148	12	47	285	12	240	2.58e-11	63.9
MS.gene052015.t1	AT2G26060	27.016	248	149	8	4	240	14	240	8.55e-11	62.4

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Find 108 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGAAGTCACACCAACT+GGG	0.643078	2.2:-3924205	None:intergenic
AAAGAAGGGGATCATTGGGA+AGG	0.667803	2.2:+3924491	MS.gene052015:CDS
AAAGTGATCATATGGACTGT+TGG	0.571303	2.2:+3924467	MS.gene052015:CDS
AAGAAGCTGTAGACGGGGAA+TGG	0.526420	2.2:+3924618	MS.gene052015:CDS
AAGAATGACGCGTCCGTCAT+AGG	0.368314	2.2:-3923988	None:intergenic
AAGACGGTAAAGTGATCATA+TGG	0.528476	2.2:+3924459	MS.gene052015:CDS
AAGCAGAGACCCAACTCATG+AGG	0.602443	2.2:-3924095	None:intergenic
AAGGTGTGGAAGCTTAGCAA+TGG	0.658169	2.2:+3924323	MS.gene052015:CDS
AATATATTGGCTGTGGCTGA+TGG	0.512420	2.2:+3924572	MS.gene052015:CDS
ACCATGATACCCTCCAAACA+GGG	0.587238	2.2:-3924538	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTTCAGATGCATAATGATT+GGG	+	chr2.2:3924373-3924392	MS.gene052015:CDS	30.0%
GAAACAACAATGTCACATTA+TGG	+	chr2.2:3924594-3924613	MS.gene052015:CDS	30.0%
GTCTTTGACAGGAAATATAT+TGG	+	chr2.2:3924559-3924578	MS.gene052015:CDS	30.0%
TATTTCGGTATTTACTGCAA+AGG	+	chr2.2:3924142-3924161	MS.gene052015:CDS	30.0%
AAGACGGTAAAGTGATCATA+TGG	+	chr2.2:3924459-3924478	MS.gene052015:CDS	35.0%
AGTAATCCATTGCAACATCA+TGG	-	chr2.2:3923806-3923825	None:intergenic	35.0%
CACTTCAGATGCATAATGAT+TGG	+	chr2.2:3924372-3924391	MS.gene052015:CDS	35.0%
CATAATCCATGATGTTGCAA+TGG	+	chr2.2:3923797-3923816	MS.gene052015:CDS	35.0%
GATTTAGGAAGTCCCAAATT+CGG	-	chr2.2:3924419-3924438	None:intergenic	35.0%
GCACTAGCAATAGTAGATTT+AGG	-	chr2.2:3924434-3924453	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	3923762	3924667	3923762	ID=MS.gene052015
chr2.2	mRNA	3923762	3924667	3923762	ID=MS.gene052015.t1;Parent=MS.gene052015
chr2.2	exon	3923762	3924667	3923762	ID=MS.gene052015.t1.exon1;Parent=MS.gene052015.t1
chr2.2	CDS	3923762	3924667	3923762	ID=cds.MS.gene052015.t1;Parent=MS.gene052015.t1

Gene Sequence

>MS.gene052015

ATGCCTTCTCAGAAGGTTGAAACCGGTCATCAAGACATAATCCATGATGTTGCAATGGATTACTATGGAAAGAGGCTAGCAACAGCATCATCGGATCACACAATTAAGATAATTGGTGTAAGCAATTCAGCTTCTCAGCATCTAGCAACTTTGACTGGTCACCAAGGGCCTGTTTGGGAGGTTGCGTGGGCTCACCCTAAGTTTGGTTCCCTTCTCGCTTCATGTTCCTATGACGGACGCGTCATTCTTTGGAAGGAGGGTAACCAAAATGAATGGATCCAAGCTCATGTCTTTGATGAGCATAAGTCGTCTGTGAATTCAGTTGCTTGGGCGCCTCATGAGTTGGGTCTCTGCTTGGCTTGTGCTTCGTCTGATGGGAATATTTCGGTATTTACTGCAAAGGCAGATGGTGGATGGGACACCTCAAGGATTGATCAAGCTCACCCAGTTGGTGTGACTTCTGTTTCTTGGGCTCCTTCAATGGCGCCGGGTGCTCTTGTTGGTTCAGGATTACTTGATCCTGTGCAGAAGCTTTGCTCTGGTGGTTGCGATAATACTGTTAAGGTGTGGAAGCTTAGCAATGGACAGTGGAAGATGGATTGCTTCCCTGCACTTCAGATGCATAATGATTGGGTTCGAGATGTTGCTTGGGCACCGAATTTGGGACTTCCTAAATCTACTATTGCTAGTGCGTCGCAAGACGGTAAAGTGATCATATGGACTGTTGGCAAAGAAGGGGATCATTGGGAAGGCAAGGTTTTGAATGATTTCAAGACCCCTGTTTGGAGGGTATCATGGTCTTTGACAGGAAATATATTGGCTGTGGCTGATGGAAACAACAATGTCACATTATGGAAAGAAGCTGTAGACGGGGAATGGCAACAGGTGACAACAGTGGAGCCATAG

Protein sequence

>MS.gene052015.t1

MPSQKVETGHQDIIHDVAMDYYGKRLATASSDHTIKIIGVSNSASQHLATLTGHQGPVWEVAWAHPKFGSLLASCSYDGRVILWKEGNQNEWIQAHVFDEHKSSVNSVAWAPHELGLCLACASSDGNISVFTAKADGGWDTSRIDQAHPVGVTSVSWAPSMAPGALVGSGLLDPVQKLCSGGCDNTVKVWKLSNGQWKMDCFPALQMHNDWVRDVAWAPNLGLPKSTIASASQDGKVIIWTVGKEGDHWEGKVLNDFKTPVWRVSWSLTGNILAVADGNNNVTLWKEAVDGEWQQVTTVEP