Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57116.t1 | XP_024641758.1 | 88.8 | 160 | 5 | 1 | 2 | 161 | 1 | 147 | 2.10E-73 | 285 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57116.t1 | P9WLH5 | 39.2 | 120 | 72 | 1 | 17 | 135 | 1 | 120 | 3.7e-18 | 92.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57116.t1 | A0A2Z6NNF5 | 69.0 | 145 | 32 | 1 | 18 | 162 | 4 | 135 | 5.5e-47 | 196.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene57116 | 0.822079 | 2.87E-53 | -1.69E-46 |
| MS.gene051199 | MS.gene57116 | 0.800332 | 1.51E-48 | -1.69E-46 |
| MS.gene051247 | MS.gene57116 | 0.812503 | 4.10E-51 | -1.69E-46 |
| MS.gene05157 | MS.gene57116 | 0.803213 | 3.88E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene57116 | 0.809298 | 2.03E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene57116 | 0.814743 | 1.32E-51 | -1.69E-46 |
| MS.gene052020 | MS.gene57116 | 0.812576 | 3.96E-51 | -1.69E-46 |
| MS.gene055359 | MS.gene57116 | 0.813543 | 2.43E-51 | -1.69E-46 |
| MS.gene055466 | MS.gene57116 | 0.807246 | 5.55E-50 | -1.69E-46 |
| MS.gene056173 | MS.gene57116 | 0.805504 | 1.29E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene57116 | 0.801014 | 1.10E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57116.t1 | AT3G01410 | 51.049 | 143 | 57 | 1 | 18 | 160 | 154 | 283 | 1.98e-44 | 148 |
| MS.gene57116.t1 | AT3G01410 | 51.049 | 143 | 57 | 1 | 18 | 160 | 154 | 283 | 1.98e-44 | 148 |
| MS.gene57116.t1 | AT5G51080 | 48.571 | 140 | 59 | 1 | 18 | 157 | 122 | 248 | 8.92e-41 | 137 |
| MS.gene57116.t1 | AT5G51080 | 47.917 | 144 | 62 | 1 | 18 | 161 | 185 | 315 | 6.45e-40 | 137 |
| MS.gene57116.t1 | AT5G51080 | 47.917 | 144 | 62 | 1 | 18 | 161 | 185 | 315 | 6.45e-40 | 137 |
| MS.gene57116.t1 | AT1G24090 | 49.650 | 143 | 59 | 1 | 18 | 160 | 216 | 345 | 1.86e-39 | 137 |
Find 38 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAACAGTTGAAAGGTTTA+TGG | 0.191806 | 6.2:+3880174 | MS.gene57116:intron |
| GCTATACTGCGTACTGAAAA+TGG | 0.195325 | 6.2:+3879626 | MS.gene57116:CDS |
| ACATCAACAATGCTTATTTA+AGG | 0.208439 | 6.2:+3880198 | MS.gene57116:CDS |
| TCTTGTATCCTTGAGTTTGA+TGG | 0.246738 | 6.2:+3879566 | MS.gene57116:CDS |
| GCAAATCGGGCCATCTTTCT+TGG | 0.285264 | 6.2:+3880590 | MS.gene57116:CDS |
| AGCTCCGGCACCAGCTGGTC+CGG | 0.304765 | 6.2:-3879606 | None:intergenic |
| GAGTATCGCGCATTGATATT+AGG | 0.335191 | 6.2:+3879797 | MS.gene57116:CDS |
| GAACTTATGCAATGAGGCTT+TGG | 0.369368 | 6.2:+3880220 | MS.gene57116:CDS |
| TATCGATTCCGCGAAGGACT+TGG | 0.410439 | 6.2:+3879752 | MS.gene57116:CDS |
| AGCTGATGCTCAAGCAAATC+GGG | 0.429319 | 6.2:+3880577 | MS.gene57116:CDS |
| AAGCTGATGCTCAAGCAAAT+CGG | 0.432683 | 6.2:+3880576 | MS.gene57116:CDS |
| CTCCGAACTTGTTATCAATC+AGG | 0.438341 | 6.2:+3879871 | MS.gene57116:CDS |
| AAGCTGTAAGGAAAGGATAT+AGG | 0.447237 | 6.2:+3879828 | MS.gene57116:CDS |
| AGCTGGTCCGGGATTCCCGC+TGG | 0.458523 | 6.2:-3879594 | None:intergenic |
| GCAATGAGGCTTTGGAGCTA+AGG | 0.469965 | 6.2:+3880228 | MS.gene57116:CDS |
| CTATACTGCGTACTGAAAAT+GGG | 0.472624 | 6.2:+3879627 | MS.gene57116:CDS |
| GGTGCAGCCAGCGGGAATCC+CGG | 0.474643 | 6.2:+3879587 | MS.gene57116:CDS |
| GCGTACTGAAAATGGGAGTC+TGG | 0.474898 | 6.2:+3879634 | MS.gene57116:CDS |
| AGCGGGAATCCCGGACCAGC+TGG | 0.480118 | 6.2:+3879596 | MS.gene57116:CDS |
| TTTGATAACCAAGTCCTTCG+CGG | 0.488713 | 6.2:-3879760 | None:intergenic |
| TTCAGTACGCAGTATAGCTC+CGG | 0.494280 | 6.2:-3879621 | None:intergenic |
| GCTCCGGCACCAGCTGGTCC+GGG | 0.509262 | 6.2:-3879605 | None:intergenic |
| TAGGACTGAAACAAGCTGTA+AGG | 0.513632 | 6.2:+3879816 | MS.gene57116:CDS |
| CAATGAGGCTTTGGAGCTAA+GGG | 0.513996 | 6.2:+3880229 | MS.gene57116:CDS |
| GACCTGATTGATAACAAGTT+CGG | 0.519027 | 6.2:-3879873 | None:intergenic |
| AGTATAGCTCCGGCACCAGC+TGG | 0.523879 | 6.2:-3879611 | None:intergenic |
| AATCGGGCCATCTTTCTTGG+AGG | 0.525253 | 6.2:+3880593 | MS.gene57116:CDS |
| AATCCCGGACCAGCTGGTGC+CGG | 0.539704 | 6.2:+3879602 | MS.gene57116:CDS |
| AGTTTGATGGTGCAGCCAGC+GGG | 0.541634 | 6.2:+3879579 | MS.gene57116:CDS |
| TATAGGAACATCACTGTCTA+CGG | 0.555751 | 6.2:+3879845 | MS.gene57116:CDS |
| GGTCTAACCTCCAAGAAAGA+TGG | 0.555833 | 6.2:-3880600 | None:intergenic |
| TTTAAGGAACTTATGCAATG+AGG | 0.557886 | 6.2:+3880214 | MS.gene57116:CDS |
| CTGAAACAAGCTGTAAGGAA+AGG | 0.589116 | 6.2:+3879821 | MS.gene57116:CDS |
| TAGCTCTATCGATTCCGCGA+AGG | 0.598150 | 6.2:+3879746 | MS.gene57116:intron |
| GAGTTTGATGGTGCAGCCAG+CGG | 0.600495 | 6.2:+3879578 | MS.gene57116:CDS |
| TGGCTGCACCATCAAACTCA+AGG | 0.610487 | 6.2:-3879574 | None:intergenic |
| GTGGTATTGCAATCGTAGAG+AGG | 0.645749 | 6.2:+3878192 | MS.gene57116:CDS |
| CTTGTCCAGATGCATCGTTG+TGG | 0.664152 | 6.2:+3878173 | MS.gene57116:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 3873214 | 3880619 | 3873214 | ID=MS.gene57116 |
| chr6.2 | mRNA | 3873214 | 3880619 | 3873214 | ID=MS.gene57116.t1;Parent=MS.gene57116 |
| chr6.2 | exon | 3873214 | 3873216 | 3873214 | ID=MS.gene57116.t1.exon1;Parent=MS.gene57116.t1 |
| chr6.2 | CDS | 3873214 | 3873216 | 3873214 | ID=cds.MS.gene57116.t1;Parent=MS.gene57116.t1 |
| chr6.2 | exon | 3878182 | 3878226 | 3878182 | ID=MS.gene57116.t1.exon2;Parent=MS.gene57116.t1 |
| chr6.2 | CDS | 3878182 | 3878226 | 3878182 | ID=cds.MS.gene57116.t1;Parent=MS.gene57116.t1 |
| chr6.2 | exon | 3879563 | 3879655 | 3879563 | ID=MS.gene57116.t1.exon3;Parent=MS.gene57116.t1 |
| chr6.2 | CDS | 3879563 | 3879655 | 3879563 | ID=cds.MS.gene57116.t1;Parent=MS.gene57116.t1 |
| chr6.2 | exon | 3879749 | 3879892 | 3879749 | ID=MS.gene57116.t1.exon4;Parent=MS.gene57116.t1 |
| chr6.2 | CDS | 3879749 | 3879892 | 3879749 | ID=cds.MS.gene57116.t1;Parent=MS.gene57116.t1 |
| chr6.2 | exon | 3880182 | 3880289 | 3880182 | ID=MS.gene57116.t1.exon5;Parent=MS.gene57116.t1 |
| chr6.2 | CDS | 3880182 | 3880289 | 3880182 | ID=cds.MS.gene57116.t1;Parent=MS.gene57116.t1 |
| chr6.2 | exon | 3880524 | 3880619 | 3880524 | ID=MS.gene57116.t1.exon6;Parent=MS.gene57116.t1 |
| chr6.2 | CDS | 3880524 | 3880619 | 3880524 | ID=cds.MS.gene57116.t1;Parent=MS.gene57116.t1 |
| Gene Sequence |
| Protein sequence |
