AlfalfaGEDB | MS.gene57154

M. sativa cultivar XinJiangDaYe / MS.gene57154

KEGG: K05658
GO: GO:0000166 GO:0003674 GO:0005215 GO:0005488 GO:0005524 GO:0005575 GO:0006810 GO:0008144 GO:0008150 GO:0015399 GO:0016021 GO:0017076 GO:0022804 GO:0022857 GO:0030554 GO:0031224 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042626 GO:0043167 GO:0043168 GO:0051179 GO:0051234 GO:0055085 GO:0097159 GO:0097367 GO:0110165 GO:1901265 GO:1901363
PFAM: PF00664.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57154.t1	XP_003618377.1	93.4	106	7	0	1	106	115	220	1.40E-44	188.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57154.t1	Q9LSJ5	72.6	106	29	0	1	106	101	206	5.8e-36	151.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57154.t1	G7KJG7	93.4	106	7	0	1	106	115	220	9.8e-45	188.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050559	MS.gene57154	0.818589	1.81E-52	-1.69E-46
MS.gene050561	MS.gene57154	0.814196	1.74E-51	-1.69E-46
MS.gene050562	MS.gene57154	0.820426	6.90E-53	-1.69E-46
MS.gene055724	MS.gene57154	0.807165	5.78E-50	-1.69E-46
MS.gene05686	MS.gene57154	0.801773	7.69E-49	-1.69E-46
MS.gene061188	MS.gene57154	0.80457	2.03E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57154.t1	MTR_6g008800	93.396	106	7	0	1	106	115	220	1.35e-63	208
MS.gene57154.t1	MTR_6g009200	85.849	106	15	0	1	106	115	220	2.57e-57	190
MS.gene57154.t1	MTR_6g009090	82.075	106	19	0	1	106	135	240	1.17e-56	184
MS.gene57154.t1	MTR_6g009030	80.189	106	21	0	1	106	115	220	6.98e-55	183
MS.gene57154.t1	MTR_6g009150	80.189	106	21	0	1	106	116	221	1.86e-54	182
MS.gene57154.t1	MTR_6g009110	80.189	106	21	0	1	106	122	227	2.95e-53	178
MS.gene57154.t1	MTR_6g009070	81.132	106	20	0	1	106	55	160	1.16e-52	177
MS.gene57154.t1	MTR_6g008820	74.528	106	27	0	1	106	103	208	4.23e-49	166
MS.gene57154.t1	MTR_7g051100	70.755	106	31	0	1	106	111	216	2.62e-45	155
MS.gene57154.t1	MTR_6g088670	46.226	106	57	0	1	106	112	217	5.42e-27	103
MS.gene57154.t1	MTR_3g086430	42.593	108	60	1	1	106	97	204	4.08e-25	98.2
MS.gene57154.t1	MTR_6g008820	60.563	71	28	0	36	106	8	78	1.34e-24	97.1
MS.gene57154.t1	MTR_4g081190	36.697	109	66	1	1	106	94	202	5.02e-20	84.0
MS.gene57154.t1	MTR_6g011680	40.566	106	62	1	1	106	117	221	9.72e-20	83.2
MS.gene57154.t1	MTR_6g008820	81.250	48	9	0	1	48	115	162	3.58e-19	81.3
MS.gene57154.t1	MTR_8g066690	37.736	106	65	1	1	106	106	210	1.34e-18	79.7
MS.gene57154.t1	MTR_5g029750	35.849	106	67	1	1	106	110	214	1.28e-17	77.0
MS.gene57154.t1	MTR_7g102070	32.075	106	71	1	1	106	180	284	2.26e-17	76.3
MS.gene57154.t1	MTR_1g063170	33.019	106	70	1	1	106	153	257	4.87e-16	72.4
MS.gene57154.t1	MTR_6g078080	33.019	106	70	1	1	106	119	223	8.24e-15	68.9
MS.gene57154.t1	MTR_1g086150	35.849	106	67	1	1	106	138	242	1.38e-14	68.2
MS.gene57154.t1	MTR_1g086080	33.962	106	69	1	1	106	143	247	4.39e-14	67.0
MS.gene57154.t1	MTR_2g018320	33.019	106	70	1	1	106	113	217	1.06e-13	65.9
MS.gene57154.t1	MTR_4g123990	36.082	97	58	2	1	97	132	224	1.54e-13	65.5
MS.gene57154.t1	MTR_1g115430	29.245	106	74	1	1	106	155	259	3.17e-13	64.3
MS.gene57154.t1	MTR_3g080220	35.616	73	47	0	1	73	148	220	4.00e-13	64.3
MS.gene57154.t1	MTR_8g022270	30.189	106	73	1	1	106	352	456	7.86e-13	63.5
MS.gene57154.t1	MTR_2g018350	37.647	85	52	1	1	85	113	196	1.26e-12	62.8
MS.gene57154.t1	MTR_4g124040	31.132	106	72	1	1	106	127	231	1.47e-12	62.4
MS.gene57154.t1	MTR_2g018530	32.075	106	71	1	1	106	123	227	1.60e-12	62.4
MS.gene57154.t1	MTR_4g124040	31.132	106	72	1	1	106	182	286	1.62e-12	62.4
MS.gene57154.t1	MTR_4g124000	33.333	87	57	1	1	87	132	217	3.10e-12	61.6
MS.gene57154.t1	MTR_3g093430	34.375	96	62	1	1	96	119	213	4.86e-12	61.2
MS.gene57154.t1	MTR_7g023340	27.358	106	76	1	1	106	162	266	5.76e-12	60.8
MS.gene57154.t1	MTR_7g023340	27.358	106	76	1	1	106	162	266	5.82e-12	60.8
MS.gene57154.t1	MTR_4g124000	33.333	87	57	1	1	87	132	217	7.84e-12	60.5
MS.gene57154.t1	MTR_3g107800	41.176	68	39	1	1	68	150	216	9.43e-12	60.5
MS.gene57154.t1	MTR_4g124050	35.849	106	67	1	1	106	150	254	2.57e-11	58.9
MS.gene57154.t1	MTR_4g124050	35.849	106	67	1	1	106	150	254	2.68e-11	58.9
MS.gene57154.t1	MTR_4g077930	28.302	106	75	1	1	106	130	234	4.69e-11	58.2
MS.gene57154.t1	MTR_4g077930	28.302	106	75	1	1	106	130	234	6.06e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57154.t1	AT3G28390	72.642	106	29	0	1	106	101	206	3.65e-49	167
MS.gene57154.t1	AT3G28345	72.642	106	29	0	1	106	113	218	5.81e-49	166
MS.gene57154.t1	AT3G28360	70.755	106	31	0	1	106	100	205	2.09e-48	164
MS.gene57154.t1	AT3G28415	69.811	106	32	0	1	106	92	197	1.35e-47	162
MS.gene57154.t1	AT3G28415	69.811	106	32	0	1	106	100	205	1.41e-47	162
MS.gene57154.t1	AT3G28380	67.925	106	34	0	1	106	113	218	1.47e-47	162
MS.gene57154.t1	AT3G28860	38.679	106	64	1	1	106	119	223	5.69e-19	80.9
MS.gene57154.t1	AT4G25960	36.792	106	66	1	1	106	155	259	5.43e-18	78.2
MS.gene57154.t1	AT2G36910	33.962	106	69	1	1	106	122	226	1.68e-17	76.6
MS.gene57154.t1	AT1G10680	33.962	106	69	1	1	106	119	223	1.55e-16	73.9
MS.gene57154.t1	AT1G10680	33.962	106	69	1	1	106	119	223	1.75e-16	73.9
MS.gene57154.t1	AT2G39480	29.245	106	74	1	1	106	168	272	2.85e-13	64.7
MS.gene57154.t1	AT3G55320	28.302	106	75	1	1	106	170	274	5.52e-13	63.9
MS.gene57154.t1	AT3G62150	31.132	106	72	1	1	106	157	261	1.19e-12	62.8
MS.gene57154.t1	AT3G62150	31.132	106	72	1	1	106	157	261	1.19e-12	62.8
MS.gene57154.t1	AT3G62150	31.132	106	72	1	1	106	157	261	1.19e-12	62.8
MS.gene57154.t1	AT2G47000	31.132	106	72	1	1	106	138	242	2.60e-12	62.0
MS.gene57154.t1	AT2G47000	31.132	106	72	1	1	106	138	242	2.60e-12	62.0
MS.gene57154.t1	AT2G47000	31.132	106	72	1	1	106	138	242	2.64e-12	62.0
MS.gene57154.t1	AT2G47000	31.132	106	72	1	1	106	138	242	2.83e-12	62.0
MS.gene57154.t1	AT2G47000	31.132	106	72	1	1	106	138	242	2.93e-12	61.6
MS.gene57154.t1	AT2G47000	31.132	106	72	1	1	106	138	242	2.93e-12	61.6
MS.gene57154.t1	AT2G47000	31.132	106	72	1	1	106	138	242	2.93e-12	61.6
MS.gene57154.t1	AT4G01830	30.189	106	73	1	1	106	107	211	5.25e-11	58.2
MS.gene57154.t1	AT1G02520	36.986	73	45	1	1	73	135	206	5.53e-11	58.2
MS.gene57154.t1	AT1G02520	36.986	73	45	1	1	73	135	206	5.53e-11	58.2
MS.gene57154.t1	AT1G02520	36.986	73	45	1	1	73	135	206	5.53e-11	58.2
MS.gene57154.t1	AT4G01820	30.189	106	73	1	1	106	102	206	6.18e-11	58.2
MS.gene57154.t1	AT1G02530	38.356	73	44	1	1	73	122	193	6.31e-11	58.2
MS.gene57154.t1	AT5G46540	32.075	106	71	1	1	106	111	215	6.77e-11	57.8
MS.gene57154.t1	AT1G02530	38.356	73	44	1	1	73	122	193	7.18e-11	57.8
MS.gene57154.t1	AT1G27940	27.358	106	76	1	1	106	125	229	8.89e-11	57.4

Find 15 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCACAAGTAGAACCATAAA+AGG	0.215344	6.2:-4361816	None:intergenic
TAGTGGCATTTGCATTATTA+TGG	0.226086	6.2:+4361772	MS.gene57154:CDS
ATGGTTCTACTTGTGATTCC+TGG	0.299087	6.2:+4361822	MS.gene57154:CDS
ATTTGCATTATTATGGAGAT+TGG	0.358984	6.2:+4361779	MS.gene57154:CDS
TTATGGAGATTGGCTATTGT+TGG	0.380950	6.2:+4361789	MS.gene57154:CDS
TAAGAACATCTTGAATTACT+AGG	0.514910	6.2:-4361359	None:intergenic
GTTCCTTGCAAGCAATATAG+TGG	0.533254	6.2:+4361755	MS.gene57154:CDS
AAGCAGCAAGAATGAGAGCT+AGG	0.536411	6.2:+4361244	None:intergenic
TAAGAAGACTTCGATGAGGT+TGG	0.557956	6.2:+4361854	MS.gene57154:CDS
ATGCCACTATATTGCTTGCA+AGG	0.565935	6.2:-4361758	None:intergenic
TCACAAGTAGAACCATAAAA+GGG	0.573707	6.2:-4361815	None:intergenic
AGAGAAGAGTACAATCAAGC+TGG	0.595050	6.2:+4361888	MS.gene57154:CDS
TCAAGATGTTCTTAGTGACA+AGG	0.618012	6.2:+4361368	MS.gene57154:CDS
GTCTTCTTATACATGTAACC+AGG	0.622083	6.2:-4361840	None:intergenic
TGTATAAGAAGACTTCGATG+AGG	0.740293	6.2:+4361850	MS.gene57154:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAAATGTGTGTTTATTTTT+CGG	+	chr6.2:4361564-4361583	MS.gene57154:intron	15.0%
!!!	AAAATATGTGTTTATTTTTC+AGG	-	chr6.2:4361639-4361658	None:intergenic	15.0%
!!	TAATTGTTTCAATGTAAAGT+CGG	-	chr6.2:4361677-4361696	None:intergenic	20.0%
!!!	GAAAAATAAACACACATTTT+TGG	-	chr6.2:4361565-4361584	None:intergenic	20.0%
!!!	TCTTCTAACTTTAAAACTAT+AGG	+	chr6.2:4361704-4361723	MS.gene57154:intron	20.0%
!	AAAATAGAACATGTCGTATT+AGG	+	chr6.2:4361446-4361465	MS.gene57154:intron	25.0%
!	ATTTGCATTATTATGGAGAT+TGG	+	chr6.2:4361779-4361798	MS.gene57154:CDS	25.0%
!	GATGCATTCATCAAAAAATT+TGG	-	chr6.2:4361732-4361751	None:intergenic	25.0%
!	TAAGAACATCTTGAATTACT+AGG	-	chr6.2:4361362-4361381	None:intergenic	25.0%
!!!	AATGCAGTATTTTTTTCAGT+TGG	-	chr6.2:4361518-4361537	None:intergenic	25.0%
!!!	ATTTTTTGATGAATGCATCT+AGG	+	chr6.2:4361733-4361752	MS.gene57154:CDS	25.0%
	ATCACAAGTAGAACCATAAA+AGG	-	chr6.2:4361819-4361838	None:intergenic	30.0%
	GTAAAGTCGGTCTTTAAATA+GGG	-	chr6.2:4361664-4361683	None:intergenic	30.0%
	TAGTGGCATTTGCATTATTA+TGG	+	chr6.2:4361772-4361791	MS.gene57154:CDS	30.0%
	TCACAAGTAGAACCATAAAA+GGG	-	chr6.2:4361818-4361837	None:intergenic	30.0%
	TGTAAAGTCGGTCTTTAAAT+AGG	-	chr6.2:4361665-4361684	None:intergenic	30.0%
!	TATTGTTGGATTCCCTTTTA+TGG	+	chr6.2:4361803-4361822	MS.gene57154:CDS	30.0%
!!	ATTTTTCGGAAGTATACTTC+TGG	+	chr6.2:4361578-4361597	MS.gene57154:intron	30.0%
!!!	AAACACACATTTTTGGTCTA+CGG	-	chr6.2:4361558-4361577	None:intergenic	30.0%
!!!	AATGCAGTATTTTTTCGTGT+TGG	+	chr6.2:4361604-4361623	MS.gene57154:intron	30.0%
	GTCTTCTTATACATGTAACC+AGG	-	chr6.2:4361843-4361862	None:intergenic	35.0%
	TCAAGATGTTCTTAGTGACA+AGG	+	chr6.2:4361368-4361387	MS.gene57154:CDS	35.0%
	TGTATAAGAAGACTTCGATG+AGG	+	chr6.2:4361850-4361869	MS.gene57154:CDS	35.0%
!	TTATGGAGATTGGCTATTGT+TGG	+	chr6.2:4361789-4361808	MS.gene57154:CDS	35.0%
	ATGCCACTATATTGCTTGCA+AGG	-	chr6.2:4361761-4361780	None:intergenic	40.0%
	GTTCCTTGCAAGCAATATAG+TGG	+	chr6.2:4361755-4361774	MS.gene57154:CDS	40.0%
	TAAGAAGACTTCGATGAGGT+TGG	+	chr6.2:4361854-4361873	MS.gene57154:CDS	40.0%
!	ATGGTTCTACTTGTGATTCC+TGG	+	chr6.2:4361822-4361841	MS.gene57154:CDS	40.0%
!	GGTCTACGGAAGTATACTTC+TGG	-	chr6.2:4361544-4361563	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	4361255	4361909	4361255	ID=MS.gene57154
chr6.2	mRNA	4361255	4361909	4361255	ID=MS.gene57154.t1;Parent=MS.gene57154
chr6.2	exon	4361255	4361389	4361255	ID=MS.gene57154.t1.exon1;Parent=MS.gene57154.t1
chr6.2	CDS	4361255	4361389	4361255	ID=cds.MS.gene57154.t1;Parent=MS.gene57154.t1
chr6.2	exon	4361726	4361909	4361726	ID=MS.gene57154.t1.exon2;Parent=MS.gene57154.t1
chr6.2	CDS	4361726	4361909	4361726	ID=cds.MS.gene57154.t1;Parent=MS.gene57154.t1

Gene Sequence

>MS.gene57154

ATGAGAGCTAGGTATCTAAAAGCAGTTCTAAGACAAGAAGTAGCATACTTTGATTTGCATGTTACAAGCACAACAGATGTCATCACTAGTGTATCTAGTGATAGCCTAGTAATTCAAGATGTTCTTAGTGACAAGGTATGAAAAAAAAAATTCATTTTACTTTATATAAAAATTTGATTTATATAGAGATGAAAATAGAACATGTCGTATTAGGTCATACTTTTATTAAAAATGTGTTTTTATTTTTCAGAAGTAAACTTCCAACTGAAAAAAATACTGCATTTGTCCAGAAGTATACTTCCGTAGACCAAAAATGTGTGTTTATTTTTCGGAAGTATACTTCTGGACAAATGCAGTATTTTTTCGTGTTGGAAGTTTACTCCTGAAAAATAAACACATATTTTTGCCCTATTTAAAGACCGACTTTACATTGAAACAATTAATTAATTTCTTCTAACTTTAAAACTATAGGTTCCAAATTTTTTGATGAATGCATCTAGGTTCCTTGCAAGCAATATAGTGGCATTTGCATTATTATGGAGATTGGCTATTGTTGGATTCCCTTTTATGGTTCTACTTGTGATTCCTGGTTACATGTATAAGAAGACTTCGATGAGGTTGGCTAGAAAGCTCAGAGAAGAGTACAATCAAGCTG

Protein sequence

>MS.gene57154.t1

MRARYLKAVLRQEVAYFDLHVTSTTDVITSVSSDSLVIQDVLSDKVPNFLMNASRFLASNIVAFALLWRLAIVGFPFMVLLVIPGYMYKKTSMRLARKLREEYNQA