AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95677


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95677.t1 MTR_7g070050 99.462 186 1 0 1 186 1 186 3.10e-137 380
MS.gene95677.t1 MTR_4g014460 68.715 179 50 3 1 178 36 209 2.85e-86 253
MS.gene95677.t1 MTR_3g071740 66.857 175 49 3 4 177 57 223 2.17e-79 236
MS.gene95677.t1 MTR_5g083270 66.102 177 50 4 4 178 37 205 6.04e-76 226
MS.gene95677.t1 MTR_8g027805 55.689 167 68 3 13 179 18 178 2.44e-64 196
MS.gene95677.t1 MTR_1g028380 56.805 169 67 3 11 179 17 179 5.66e-64 196
MS.gene95677.t1 MTR_3g090980 54.913 173 72 3 7 179 15 181 9.91e-63 192
MS.gene95677.t1 MTR_5g030500 58.182 165 66 2 13 176 44 206 1.32e-60 188
MS.gene95677.t1 MTR_1g016480 52.023 173 78 3 13 185 39 206 1.84e-59 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95677.t1 AT2G38310 74.576 177 36 2 5 180 31 199 5.70e-85 249
MS.gene95677.t1 AT2G40330 65.882 170 54 2 9 177 44 210 1.24e-80 239
MS.gene95677.t1 AT5G05440 66.867 166 46 3 9 173 41 198 6.79e-77 229
MS.gene95677.t1 AT1G01360 56.471 170 68 2 16 185 24 187 2.29e-66 201
MS.gene95677.t1 AT5G53160 52.809 178 77 3 2 179 9 179 2.89e-64 196
MS.gene95677.t1 AT4G01026 56.707 164 65 3 17 180 27 184 2.19e-62 192
MS.gene95677.t1 AT2G26040 53.571 168 69 2 8 172 17 178 2.80e-59 184
MS.gene95677.t1 AT4G17870 51.111 180 82 2 1 179 5 179 5.56e-59 183
MS.gene95677.t1 AT4G27920 47.159 176 88 2 11 186 12 182 1.18e-57 179
MS.gene95677.t1 AT1G73000 50.000 168 78 3 11 172 34 201 3.87e-54 171
MS.gene95677.t1 AT5G45860 55.758 165 60 2 15 179 6 157 7.25e-51 161
MS.gene95677.t1 AT5G46790 53.179 173 78 2 7 178 38 208 4.96e-49 159
MS.gene95677.t1 AT5G46790 53.179 173 78 2 7 178 38 208 4.96e-49 159
MS.gene95677.t1 AT5G45870 55.556 153 61 1 26 178 10 155 5.21e-48 154
MS.gene95677.t1 AT4G18620 51.553 161 67 3 24 178 7 162 1.44e-39 132
MS.gene95677.t1 AT5G53160 53.571 112 50 2 2 113 9 118 2.37e-37 127

Find 70 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGAAATTTGCTGAGAATT+TGG 0.111678 7.4:-33770581 MS.gene95677:CDS
TGGTAGCGGGCGATGGCGTC+GGG 0.286221 7.4:+33771066 None:intergenic
CGGCGGAGCAACACTGGTTT+GGG 0.304384 7.4:+33771028 None:intergenic
GTGGTAGCGGGCGATGGCGT+CGG 0.350975 7.4:+33771065 None:intergenic
GTATCTTCAGTGGTGTTTCC+AGG 0.355878 7.4:+33770649 None:intergenic
ACGGCGGAGCAACACTGGTT+TGG 0.359142 7.4:+33771027 None:intergenic
GGGACGGTGGTTGTTGGATT+CGG 0.391057 7.4:+33771087 None:intergenic
GCGTCGGGGACGGTGGTTGT+TGG 0.398154 7.4:+33771081 None:intergenic
GTCATCAGCTTCAGCATGAT+TGG 0.416594 7.4:-33770796 MS.gene95677:CDS
CGTTCGGTGCTGACGGCGGC+GGG 0.423682 7.4:+33770847 None:intergenic
TCTTCGTCGGAGATCGGACT+AGG 0.424642 7.4:+33770727 None:intergenic
GACTGCGTGGGTGTGGTAGC+GGG 0.430031 7.4:+33771053 None:intergenic
CAAATTTCGCGAGAGATTGA+AGG 0.431244 7.4:+33770593 None:intergenic
AGACTGCGTGGGTGTGGTAG+CGG 0.434046 7.4:+33771052 None:intergenic
ACGTTCGGTGCTGACGGCGG+CGG 0.440590 7.4:+33770846 None:intergenic
GCGGGCGATGGCGTCGGGGA+CGG 0.442801 7.4:+33771071 None:intergenic
GCTGACGGCGGCGGGGAGGC+CGG 0.449916 7.4:+33770855 None:intergenic
GCATGATTGGCGGTGATCAC+CGG 0.466189 7.4:-33770783 MS.gene95677:CDS
CGTGAAGTCCGCGTCATCTC+CGG 0.470592 7.4:-33770874 MS.gene95677:CDS
TTGGGGAGACTGCGTGGGTG+TGG 0.471898 7.4:+33771046 None:intergenic
GTGGGTGTGGTAGCGGGCGA+TGG 0.478652 7.4:+33771059 None:intergenic
GTCTAGGACTTCGAGACGTT+CGG 0.489158 7.4:+33770831 None:intergenic
CGGAGATGACGCGGACTTCA+CGG 0.493833 7.4:+33770875 None:intergenic
CCGCACCCGTCTCCACCGTC+TGG 0.503156 7.4:-33770990 MS.gene95677:CDS
GGCGATGGCGTCGGGGACGG+TGG 0.507167 7.4:+33771074 None:intergenic
AACGGACCAGACGGTGGAGA+CGG 0.510351 7.4:+33770984 None:intergenic
TACGTTGTTGATGTTCCACC+TGG 0.511693 7.4:-33770667 MS.gene95677:CDS
TGTTTGTTGATACTATTCTT+CGG 0.519630 7.4:-33770621 MS.gene95677:CDS
ACGGACCAGACGGTGGAGAC+GGG 0.521318 7.4:+33770985 None:intergenic
AGTCCGATCTCCGACGAAGA+TGG 0.524898 7.4:-33770724 MS.gene95677:CDS
TTCGAGACGTTCGGTGCTGA+CGG 0.530715 7.4:+33770840 None:intergenic
TTGCCATCTTCGTCGGAGAT+CGG 0.532468 7.4:+33770721 None:intergenic
TGCCATGTCATCCTCGGCGA+CGG 0.538063 7.4:-33770913 MS.gene95677:CDS
CGGGGAGGCCGGAGATGACG+CGG 0.540759 7.4:+33770866 None:intergenic
GAAGATGGCAACCACCGCTC+CGG 0.541680 7.4:-33770709 MS.gene95677:CDS
AGACGGGTGCGGCGATATGT+TGG 0.545462 7.4:+33771001 None:intergenic
CGGTGCTGACGGCGGCGGGG+AGG 0.548394 7.4:+33770851 None:intergenic
GGATGACGGCGGAGCAACAC+TGG 0.549088 7.4:+33771022 None:intergenic
GTTCGGTGCTGACGGCGGCG+GGG 0.549616 7.4:+33770848 None:intergenic
ATCCTCGGCGACGGCAACGT+CGG 0.566845 7.4:-33770904 MS.gene95677:CDS
CAACCACCGTGCCGGAGCGG+TGG 0.569837 7.4:+33770698 None:intergenic
TGACGTGACGTTCATCGTCT+AGG 0.571433 7.4:+33770815 None:intergenic
AAAAGCTGCCATGTCATCCT+CGG 0.573277 7.4:-33770919 MS.gene95677:CDS
ATCAGCTTCAGCATGATTGG+CGG 0.579196 7.4:-33770793 MS.gene95677:CDS
GGATTCAACAACCACCGTGC+CGG 0.581765 7.4:+33770690 None:intergenic
GCGGTGGTTGCCATCTTCGT+CGG 0.582833 7.4:+33770714 None:intergenic
TGCCGTCGCCGAGGATGACA+TGG 0.583389 7.4:+33770911 None:intergenic
TGCCGACGTTGCCGTCGCCG+AGG 0.587984 7.4:+33770902 None:intergenic
ACTGGTTTGGGGAGACTGCG+TGG 0.592349 7.4:+33771040 None:intergenic
CTTCGTCGGAGATCGGACTA+GGG 0.595738 7.4:+33770728 None:intergenic
CCAGACGGTGGAGACGGGTG+CGG 0.600624 7.4:+33770990 None:intergenic
TCTTCAGTGGTGTTTCCAGG+TGG 0.600688 7.4:+33770652 None:intergenic
TGCGGCGATATGTTGGATGA+CGG 0.601558 7.4:+33771008 None:intergenic
ACGGACAACGGACCAGACGG+TGG 0.603928 7.4:+33770978 None:intergenic
TGGCAACCACCGCTCCGGCA+CGG 0.610622 7.4:-33770704 MS.gene95677:CDS
GCGACGGACAACGGACCAGA+CGG 0.611981 7.4:+33770975 None:intergenic
CTGGTTTGGGGAGACTGCGT+GGG 0.619515 7.4:+33771041 None:intergenic
GGTGAAGAGTGGTGACAGAA+CGG 0.622514 7.4:+33770749 None:intergenic
ATTGTCGAAGCGACGGACAA+CGG 0.628400 7.4:+33770966 None:intergenic
AAACACACATGTATCTTCAG+TGG 0.628787 7.4:+33770639 None:intergenic
CAGAACGGTAATTAGCAAGC+CGG 0.629081 7.4:+33770764 None:intergenic
CAACCACCGCTCCGGCACGG+TGG 0.633877 7.4:-33770701 MS.gene95677:CDS
CAACAACCACCGTGCCGGAG+CGG 0.635412 7.4:+33770695 None:intergenic
GGTAGCGGGCGATGGCGTCG+GGG 0.638452 7.4:+33771067 None:intergenic
GAGACGTTCGGTGCTGACGG+CGG 0.644895 7.4:+33770843 None:intergenic
GATCGGACTAGGGTGAAGAG+TGG 0.660402 7.4:+33770738 None:intergenic
CTTGTGGATTGTCGAAGCGA+CGG 0.665023 7.4:+33770959 None:intergenic
GGCGGAGCAACACTGGTTTG+GGG 0.687344 7.4:+33771029 None:intergenic
GGCGATATGTTGGATGACGG+CGG 0.710240 7.4:+33771011 None:intergenic
AGGTGGAACATCAACAACGT+AGG 0.714920 7.4:+33770669 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTGTTGATACTATTCTT+CGG - chr7.4:33771027-33771046 MS.gene95677:CDS 25.0%
AAACACACATGTATCTTCAG+TGG + chr7.4:33771012-33771031 None:intergenic 35.0%
! GCTTTTGACGAAGTTTTTGT+AGG + chr7.4:33770715-33770734 None:intergenic 35.0%
CAAATTTCGCGAGAGATTGA+AGG + chr7.4:33771058-33771077 None:intergenic 40.0%
! ACGAAGTTTTTGTAGGCTTG+TGG + chr7.4:33770708-33770727 None:intergenic 40.0%
!! CGCGAAATTTGCTGAGAATT+TGG - chr7.4:33771067-33771086 MS.gene95677:CDS 40.0%
AAAAGCTGCCATGTCATCCT+CGG - chr7.4:33770729-33770748 MS.gene95677:CDS 45.0%
AGGTGGAACATCAACAACGT+AGG + chr7.4:33770982-33771001 None:intergenic 45.0%
ATCAGCTTCAGCATGATTGG+CGG - chr7.4:33770855-33770874 MS.gene95677:CDS 45.0%
CAGAACGGTAATTAGCAAGC+CGG + chr7.4:33770887-33770906 None:intergenic 45.0%
GTATCTTCAGTGGTGTTTCC+AGG + chr7.4:33771002-33771021 None:intergenic 45.0%
GTCATCAGCTTCAGCATGAT+TGG - chr7.4:33770852-33770871 MS.gene95677:CDS 45.0%
TACGTTGTTGATGTTCCACC+TGG - chr7.4:33770981-33771000 MS.gene95677:CDS 45.0%
ATTGTCGAAGCGACGGACAA+CGG + chr7.4:33770685-33770704 None:intergenic 50.0%
GGTGAAGAGTGGTGACAGAA+CGG + chr7.4:33770902-33770921 None:intergenic 50.0%
TCTTCAGTGGTGTTTCCAGG+TGG + chr7.4:33770999-33771018 None:intergenic 50.0%
TGACGTGACGTTCATCGTCT+AGG + chr7.4:33770836-33770855 None:intergenic 50.0%
TGCGGCGATATGTTGGATGA+CGG + chr7.4:33770643-33770662 None:intergenic 50.0%
TTGCCATCTTCGTCGGAGAT+CGG + chr7.4:33770930-33770949 None:intergenic 50.0%
! CTTGTGGATTGTCGAAGCGA+CGG + chr7.4:33770692-33770711 None:intergenic 50.0%
! GTCTAGGACTTCGAGACGTT+CGG + chr7.4:33770820-33770839 None:intergenic 50.0%
AGTCCGATCTCCGACGAAGA+TGG - chr7.4:33770924-33770943 MS.gene95677:CDS 55.0%
CTTCGTCGGAGATCGGACTA+GGG + chr7.4:33770923-33770942 None:intergenic 55.0%
GCATGATTGGCGGTGATCAC+CGG - chr7.4:33770865-33770884 MS.gene95677:CDS 55.0%
GGATTCAACAACCACCGTGC+CGG + chr7.4:33770961-33770980 None:intergenic 55.0%
GGGACGGTGGTTGTTGGATT+CGG + chr7.4:33770564-33770583 None:intergenic 55.0%
TCTTCGTCGGAGATCGGACT+AGG + chr7.4:33770924-33770943 None:intergenic 55.0%
! GATCGGACTAGGGTGAAGAG+TGG + chr7.4:33770913-33770932 None:intergenic 55.0%
! GGCGATATGTTGGATGACGG+CGG + chr7.4:33770640-33770659 None:intergenic 55.0%
!! TTCGAGACGTTCGGTGCTGA+CGG + chr7.4:33770811-33770830 None:intergenic 55.0%
AACGGACCAGACGGTGGAGA+CGG + chr7.4:33770667-33770686 None:intergenic 60.0%
AGACGGGTGCGGCGATATGT+TGG + chr7.4:33770650-33770669 None:intergenic 60.0%
AGACTGCGTGGGTGTGGTAG+CGG + chr7.4:33770599-33770618 None:intergenic 60.0%
CGGAGATGACGCGGACTTCA+CGG + chr7.4:33770776-33770795 None:intergenic 60.0%
CGTGAAGTCCGCGTCATCTC+CGG - chr7.4:33770774-33770793 MS.gene95677:CDS 60.0%
GAAGATGGCAACCACCGCTC+CGG - chr7.4:33770939-33770958 MS.gene95677:CDS 60.0%
GCGGTGGTTGCCATCTTCGT+CGG + chr7.4:33770937-33770956 None:intergenic 60.0%
TGCCATGTCATCCTCGGCGA+CGG - chr7.4:33770735-33770754 MS.gene95677:CDS 60.0%
! ACGGCGGAGCAACACTGGTT+TGG + chr7.4:33770624-33770643 None:intergenic 60.0%
!! ACTGGTTTGGGGAGACTGCG+TGG + chr7.4:33770611-33770630 None:intergenic 60.0%
!! CGGCGGAGCAACACTGGTTT+GGG + chr7.4:33770623-33770642 None:intergenic 60.0%
!! CTGGTTTGGGGAGACTGCGT+GGG + chr7.4:33770610-33770629 None:intergenic 60.0%
!! GGCGGAGCAACACTGGTTTG+GGG + chr7.4:33770622-33770641 None:intergenic 60.0%
ACGGACAACGGACCAGACGG+TGG + chr7.4:33770673-33770692 None:intergenic 65.0%
ACGGACCAGACGGTGGAGAC+GGG + chr7.4:33770666-33770685 None:intergenic 65.0%
ATCCTCGGCGACGGCAACGT+CGG - chr7.4:33770744-33770763 MS.gene95677:CDS 65.0%
CAACAACCACCGTGCCGGAG+CGG + chr7.4:33770956-33770975 None:intergenic 65.0%
GACTGCGTGGGTGTGGTAGC+GGG + chr7.4:33770598-33770617 None:intergenic 65.0%
GCGACGGACAACGGACCAGA+CGG + chr7.4:33770676-33770695 None:intergenic 65.0%
TGCCGTCGCCGAGGATGACA+TGG + chr7.4:33770740-33770759 None:intergenic 65.0%
TTGGGGAGACTGCGTGGGTG+TGG + chr7.4:33770605-33770624 None:intergenic 65.0%
! GGATGACGGCGGAGCAACAC+TGG + chr7.4:33770629-33770648 None:intergenic 65.0%
!! GAGACGTTCGGTGCTGACGG+CGG + chr7.4:33770808-33770827 None:intergenic 65.0%
CCAGACGGTGGAGACGGGTG+CGG + chr7.4:33770661-33770680 None:intergenic 70.0%
GCGTCGGGGACGGTGGTTGT+TGG + chr7.4:33770570-33770589 None:intergenic 70.0%
TGGCAACCACCGCTCCGGCA+CGG - chr7.4:33770944-33770963 MS.gene95677:CDS 70.0%
!! ACGTTCGGTGCTGACGGCGG+CGG + chr7.4:33770805-33770824 None:intergenic 70.0%
!! GTGGGTGTGGTAGCGGGCGA+TGG + chr7.4:33770592-33770611 None:intergenic 70.0%
!! GTGGTAGCGGGCGATGGCGT+CGG + chr7.4:33770586-33770605 None:intergenic 70.0%
!! TGGTAGCGGGCGATGGCGTC+GGG + chr7.4:33770585-33770604 None:intergenic 70.0%
CAACCACCGCTCCGGCACGG+TGG - chr7.4:33770947-33770966 MS.gene95677:CDS 75.0%
CAACCACCGTGCCGGAGCGG+TGG + chr7.4:33770953-33770972 None:intergenic 75.0%
CCGCACCCGTCTCCACCGTC+TGG - chr7.4:33770658-33770677 MS.gene95677:CDS 75.0%
CGGGGAGGCCGGAGATGACG+CGG + chr7.4:33770785-33770804 None:intergenic 75.0%
TGCCGACGTTGCCGTCGCCG+AGG + chr7.4:33770749-33770768 None:intergenic 75.0%
!! CGTTCGGTGCTGACGGCGGC+GGG + chr7.4:33770804-33770823 None:intergenic 75.0%
!! GGTAGCGGGCGATGGCGTCG+GGG + chr7.4:33770584-33770603 None:intergenic 75.0%
!! GTTCGGTGCTGACGGCGGCG+GGG + chr7.4:33770803-33770822 None:intergenic 75.0%
!! GCGGGCGATGGCGTCGGGGA+CGG + chr7.4:33770580-33770599 None:intergenic 80.0%
!! GGCGATGGCGTCGGGGACGG+TGG + chr7.4:33770577-33770596 None:intergenic 80.0%
! GCTGACGGCGGCGGGGAGGC+CGG + chr7.4:33770796-33770815 None:intergenic 85.0%
!!! CGGTGCTGACGGCGGCGGGG+AGG + chr7.4:33770800-33770819 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 33770555 33771115 33770555 ID=MS.gene95677
chr7.4 mRNA 33770555 33771115 33770555 ID=MS.gene95677.t1;Parent=MS.gene95677
chr7.4 exon 33770555 33771115 33770555 ID=MS.gene95677.t1.exon1;Parent=MS.gene95677.t1
chr7.4 CDS 33770555 33771115 33770555 ID=cds.MS.gene95677.t1;Parent=MS.gene95677.t1
Gene Sequence

>MS.gene95677

ATGTTACCGAATCCAACAACCACCGTCCCCGACGCCATCGCCCGCTACCACACCCACGCAGTCTCCCCAAACCAGTGTTGCTCCGCCGTCATCCAACATATCGCCGCACCCGTCTCCACCGTCTGGTCCGTTGTCCGTCGCTTCGACAATCCACAAGCCTACAAAAACTTCGTCAAAAGCTGCCATGTCATCCTCGGCGACGGCAACGTCGGCACTCTCCGTGAAGTCCGCGTCATCTCCGGCCTCCCCGCCGCCGTCAGCACCGAACGTCTCGAAGTCCTAGACGATGAACGTCACGTCATCAGCTTCAGCATGATTGGCGGTGATCACCGGCTTGCTAATTACCGTTCTGTCACCACTCTTCACCCTAGTCCGATCTCCGACGAAGATGGCAACCACCGCTCCGGCACGGTGGTTGTTGAATCCTACGTTGTTGATGTTCCACCTGGAAACACCACTGAAGATACATGTGTGTTTGTTGATACTATTCTTCGGTGCAACCTTCAATCTCTCGCGAAATTTGCTGAGAATTTGGCTTCAACGAGATCAAATCAACGATAA

Protein sequence

>MS.gene95677.t1

MLPNPTTTVPDAIARYHTHAVSPNQCCSAVIQHIAAPVSTVWSVVRRFDNPQAYKNFVKSCHVILGDGNVGTLREVRVISGLPAAVSTERLEVLDDERHVISFSMIGGDHRLANYRSVTTLHPSPISDEDGNHRSGTVVVESYVVDVPPGNTTEDTCVFVDTILRCNLQSLAKFAENLASTRSNQR