AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99506


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99506.t1 MTR_7g070050 98.925 186 2 0 1 186 1 186 1.25e-136 379
MS.gene99506.t1 MTR_4g014460 68.156 179 51 3 1 178 36 209 5.99e-86 252
MS.gene99506.t1 MTR_3g071740 66.286 175 50 3 4 177 57 223 5.16e-79 235
MS.gene99506.t1 MTR_5g083270 66.667 177 49 4 4 178 37 205 2.92e-77 229
MS.gene99506.t1 MTR_8g027805 55.090 167 69 3 13 179 18 178 3.54e-63 193
MS.gene99506.t1 MTR_1g028380 56.213 169 68 3 11 179 17 179 7.13e-63 192
MS.gene99506.t1 MTR_5g030500 58.788 165 65 2 13 176 44 206 4.76e-62 191
MS.gene99506.t1 MTR_3g090980 54.335 173 73 3 7 179 15 181 1.18e-61 190
MS.gene99506.t1 MTR_1g016480 52.601 173 77 3 13 185 39 206 4.47e-61 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99506.t1 AT2G38310 75.141 177 35 2 5 180 31 199 1.32e-86 253
MS.gene99506.t1 AT2G40330 67.647 170 51 3 9 177 44 210 2.62e-82 243
MS.gene99506.t1 AT5G05440 66.265 166 47 3 9 173 41 198 2.64e-76 227
MS.gene99506.t1 AT1G01360 55.882 170 69 2 16 185 24 187 3.01e-65 199
MS.gene99506.t1 AT5G53160 52.247 178 78 3 2 179 9 179 3.09e-63 194
MS.gene99506.t1 AT4G01026 56.707 164 65 3 17 180 27 184 5.99e-62 191
MS.gene99506.t1 AT2G26040 54.762 168 67 2 8 172 17 178 2.09e-61 189
MS.gene99506.t1 AT4G17870 51.667 180 81 2 1 179 5 179 3.64e-60 186
MS.gene99506.t1 AT4G27920 46.591 176 89 2 11 186 12 182 2.17e-56 176
MS.gene99506.t1 AT1G73000 50.595 168 77 3 11 172 34 201 5.24e-56 176
MS.gene99506.t1 AT5G45860 55.758 165 60 2 15 179 6 157 3.65e-51 162
MS.gene99506.t1 AT5G46790 53.757 173 77 2 7 178 38 208 2.15e-50 162
MS.gene99506.t1 AT5G46790 53.757 173 77 2 7 178 38 208 2.15e-50 162
MS.gene99506.t1 AT5G45870 56.209 153 60 1 26 178 10 155 1.27e-49 158
MS.gene99506.t1 AT4G18620 51.553 161 67 3 24 178 7 162 7.27e-40 133
MS.gene99506.t1 AT5G53160 52.679 112 51 2 2 113 9 118 2.88e-36 124

Find 69 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGAAATTTGCTGAGAATT+TGG 0.108745 7.1:-32178536 MS.gene99506:CDS
TGGTAGCGGGCGATGGCGTC+GGG 0.286221 7.1:+32179021 None:intergenic
GGGACGGTGGTTGTTGGGTT+TGG 0.316747 7.1:+32179042 None:intergenic
GTGGTAGCGGGCGATGGCGT+CGG 0.350975 7.1:+32179020 None:intergenic
GCGTCGGGGACGGTGGTTGT+TGG 0.355449 7.1:+32179036 None:intergenic
ACGGCGGAGCAACACTGGTT+GGG 0.355563 7.1:+32178982 None:intergenic
GTATCTTCAGTGGTGTTTCC+AGG 0.355878 7.1:+32178604 None:intergenic
CGTCGGGGACGGTGGTTGTT+GGG 0.383221 7.1:+32179037 None:intergenic
TCTTCGTCGGAGATCGGACT+AGG 0.424642 7.1:+32178682 None:intergenic
GACTGCGTGGGTGTGGTAGC+GGG 0.430031 7.1:+32179008 None:intergenic
CAAATTTCGCGAGAGATTGA+AGG 0.431244 7.1:+32178548 None:intergenic
AGACTGCGTGGGTGTGGTAG+CGG 0.434046 7.1:+32179007 None:intergenic
GCTGACAGCGGCGGGGAGGC+CGG 0.435041 7.1:+32178810 None:intergenic
AGACGGGTGCGGCGATTTGT+TGG 0.442519 7.1:+32178956 None:intergenic
GCGGGCGATGGCGTCGGGGA+CGG 0.442801 7.1:+32179026 None:intergenic
CGTTCGGTGCTGACAGCGGC+GGG 0.446228 7.1:+32178802 None:intergenic
CGGCGGAGCAACACTGGTTG+GGG 0.450760 7.1:+32178983 None:intergenic
GTGGGTGTGGTAGCGGGCGA+TGG 0.478652 7.1:+32179014 None:intergenic
GTCTAGGACTTCGAGACGTT+CGG 0.489158 7.1:+32178786 None:intergenic
CGTGAAGTCCGTGTCATCTC+CGG 0.492295 7.1:-32178829 MS.gene99506:CDS
CGGAGATGACACGGACTTCA+CGG 0.493471 7.1:+32178830 None:intergenic
GCATGATCGGTGGTGATCAC+CGG 0.500077 7.1:-32178738 MS.gene99506:CDS
CCGCACCCGTCTCCACCGTC+TGG 0.503156 7.1:-32178945 MS.gene99506:CDS
GGCGATGGCGTCGGGGACGG+TGG 0.507167 7.1:+32179029 None:intergenic
TACGTTGTTGATGTTCCACC+TGG 0.511693 7.1:-32178622 MS.gene99506:CDS
GTCATCAGCTTCAGCATGAT+CGG 0.512224 7.1:-32178751 MS.gene99506:CDS
TGTTTGTTGATACTATTCTT+CGG 0.519630 7.1:-32178576 MS.gene99506:CDS
GACGGCGGAGCAACACTGGT+TGG 0.519929 7.1:+32178981 None:intergenic
TTGCCATCTTCGTCGGAGAT+CGG 0.523840 7.1:+32178676 None:intergenic
AGTCCGATCTCCGACGAAGA+TGG 0.524898 7.1:-32178679 MS.gene99506:CDS
ACGAAGTGTTTGTAGGCTTG+TGG 0.526533 7.1:+32178898 None:intergenic
TACGGACCAGACGGTGGAGA+CGG 0.531050 7.1:+32178939 None:intergenic
TGCCGTCACCGAGGATTACA+TGG 0.548078 7.1:+32178866 None:intergenic
ACGGACCAGACGGTGGAGAC+GGG 0.550345 7.1:+32178940 None:intergenic
GCGTTGGTTGCCATCTTCGT+CGG 0.552270 7.1:+32178669 None:intergenic
CAACCACCGTGCCGGAGCGT+TGG 0.554675 7.1:+32178653 None:intergenic
TGACATGACGTTCATCGTCT+AGG 0.555135 7.1:+32178770 None:intergenic
CGGGGAGGCCGGAGATGACA+CGG 0.556039 7.1:+32178821 None:intergenic
TGCGGCGATTTGTTGGATGA+CGG 0.556569 7.1:+32178963 None:intergenic
GAAGATGGCAACCAACGCTC+CGG 0.557103 7.1:-32178664 MS.gene99506:CDS
ATTGTCGAAGCGACGGACTA+CGG 0.565639 7.1:+32178921 None:intergenic
ACGTTCGGTGCTGACAGCGG+CGG 0.570809 7.1:+32178801 None:intergenic
TGCCATGTAATCCTCGGTGA+CGG 0.575327 7.1:-32178868 MS.gene99506:CDS
CGGTGCTGACAGCGGCGGGG+AGG 0.581007 7.1:+32178806 None:intergenic
GGATTCAACAACCACCGTGC+CGG 0.581765 7.1:+32178645 None:intergenic
AAAAGCTGCCATGTAATCCT+CGG 0.586229 7.1:-32178874 MS.gene99506:CDS
GGATGACGGCGGAGCAACAC+TGG 0.592996 7.1:+32178977 None:intergenic
CTTCGTCGGAGATCGGACTA+GGG 0.595738 7.1:+32178683 None:intergenic
CCAGACGGTGGAGACGGGTG+CGG 0.600624 7.1:+32178945 None:intergenic
TCTTCAGTGGTGTTTCCAGG+TGG 0.600688 7.1:+32178607 None:intergenic
GGTGAAGAGTGGTGACAGAA+CGG 0.622514 7.1:+32178704 None:intergenic
GCGACGGACTACGGACCAGA+CGG 0.625454 7.1:+32178930 None:intergenic
TGGCAACCAACGCTCCGGCA+CGG 0.627638 7.1:-32178659 MS.gene99506:CDS
AAACACACATGTATCTTCAG+TGG 0.628787 7.1:+32178594 None:intergenic
TCGTTGATTTGATCTCGTTG+AGG 0.631063 7.1:+32178513 None:intergenic
GGTAGCGGGCGATGGCGTCG+GGG 0.638452 7.1:+32179022 None:intergenic
GGCGGAGCAACACTGGTTGG+GGG 0.641966 7.1:+32178984 None:intergenic
ACGGACTACGGACCAGACGG+TGG 0.644893 7.1:+32178933 None:intergenic
GAGACGTTCGGTGCTGACAG+CGG 0.650293 7.1:+32178798 None:intergenic
ATCCTCGGTGACGGCAACGT+CGG 0.652459 7.1:-32178859 MS.gene99506:CDS
ATCAGCTTCAGCATGATCGG+TGG 0.655710 7.1:-32178748 MS.gene99506:CDS
GTTCGGTGCTGACAGCGGCG+GGG 0.655778 7.1:+32178803 None:intergenic
CAGAACGGTAGTTAGCAAGC+CGG 0.656130 7.1:+32178719 None:intergenic
GATCGGACTAGGGTGAAGAG+TGG 0.660402 7.1:+32178693 None:intergenic
CTTGTGGATTGTCGAAGCGA+CGG 0.660521 7.1:+32178914 None:intergenic
CAACCAACGCTCCGGCACGG+TGG 0.673035 7.1:-32178656 MS.gene99506:CDS
GGCGATTTGTTGGATGACGG+CGG 0.674940 7.1:+32178966 None:intergenic
TGCCGACGTTGCCGTCACCG+AGG 0.697315 7.1:+32178857 None:intergenic
AGGTGGAACATCAACAACGT+AGG 0.714920 7.1:+32178624 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTGTTGATACTATTCTT+CGG - chr7.1:32178982-32179001 MS.gene99506:CDS 25.0%
AAACACACATGTATCTTCAG+TGG + chr7.1:32178967-32178986 None:intergenic 35.0%
AAAAGCTGCCATGTAATCCT+CGG - chr7.1:32178684-32178703 MS.gene99506:CDS 40.0%
CAAATTTCGCGAGAGATTGA+AGG + chr7.1:32179013-32179032 None:intergenic 40.0%
!! CGCGAAATTTGCTGAGAATT+TGG - chr7.1:32179022-32179041 MS.gene99506:CDS 40.0%
!! GCTTTTGACGAAGTGTTTGT+AGG + chr7.1:32178670-32178689 None:intergenic 40.0%
!! TCGTTGATTTGATCTCGTTG+AGG + chr7.1:32179048-32179067 None:intergenic 40.0%
AGGTGGAACATCAACAACGT+AGG + chr7.1:32178937-32178956 None:intergenic 45.0%
GTATCTTCAGTGGTGTTTCC+AGG + chr7.1:32178957-32178976 None:intergenic 45.0%
GTCATCAGCTTCAGCATGAT+CGG - chr7.1:32178807-32178826 MS.gene99506:CDS 45.0%
TACGTTGTTGATGTTCCACC+TGG - chr7.1:32178936-32178955 MS.gene99506:CDS 45.0%
TGACATGACGTTCATCGTCT+AGG + chr7.1:32178791-32178810 None:intergenic 45.0%
! ACGAAGTGTTTGTAGGCTTG+TGG + chr7.1:32178663-32178682 None:intergenic 45.0%
ATCAGCTTCAGCATGATCGG+TGG - chr7.1:32178810-32178829 MS.gene99506:CDS 50.0%
CAGAACGGTAGTTAGCAAGC+CGG + chr7.1:32178842-32178861 None:intergenic 50.0%
GGTGAAGAGTGGTGACAGAA+CGG + chr7.1:32178857-32178876 None:intergenic 50.0%
TCTTCAGTGGTGTTTCCAGG+TGG + chr7.1:32178954-32178973 None:intergenic 50.0%
TGCCATGTAATCCTCGGTGA+CGG - chr7.1:32178690-32178709 MS.gene99506:CDS 50.0%
TTGCCATCTTCGTCGGAGAT+CGG + chr7.1:32178885-32178904 None:intergenic 50.0%
! CTTGTGGATTGTCGAAGCGA+CGG + chr7.1:32178647-32178666 None:intergenic 50.0%
! GTCTAGGACTTCGAGACGTT+CGG + chr7.1:32178775-32178794 None:intergenic 50.0%
!! ATTGTCGAAGCGACGGACTA+CGG + chr7.1:32178640-32178659 None:intergenic 50.0%
!! TGCGGCGATTTGTTGGATGA+CGG + chr7.1:32178598-32178617 None:intergenic 50.0%
AGTCCGATCTCCGACGAAGA+TGG - chr7.1:32178879-32178898 MS.gene99506:CDS 55.0%
CGGAGATGACACGGACTTCA+CGG + chr7.1:32178731-32178750 None:intergenic 55.0%
CGTGAAGTCCGTGTCATCTC+CGG - chr7.1:32178729-32178748 MS.gene99506:CDS 55.0%
CTTCGTCGGAGATCGGACTA+GGG + chr7.1:32178878-32178897 None:intergenic 55.0%
GAAGATGGCAACCAACGCTC+CGG - chr7.1:32178894-32178913 MS.gene99506:CDS 55.0%
GCATGATCGGTGGTGATCAC+CGG - chr7.1:32178820-32178839 MS.gene99506:CDS 55.0%
GGATTCAACAACCACCGTGC+CGG + chr7.1:32178916-32178935 None:intergenic 55.0%
TCTTCGTCGGAGATCGGACT+AGG + chr7.1:32178879-32178898 None:intergenic 55.0%
TGCCGTCACCGAGGATTACA+TGG + chr7.1:32178695-32178714 None:intergenic 55.0%
! GATCGGACTAGGGTGAAGAG+TGG + chr7.1:32178868-32178887 None:intergenic 55.0%
!! GCGTTGGTTGCCATCTTCGT+CGG + chr7.1:32178892-32178911 None:intergenic 55.0%
!! GGCGATTTGTTGGATGACGG+CGG + chr7.1:32178595-32178614 None:intergenic 55.0%
AGACGGGTGCGGCGATTTGT+TGG + chr7.1:32178605-32178624 None:intergenic 60.0%
AGACTGCGTGGGTGTGGTAG+CGG + chr7.1:32178554-32178573 None:intergenic 60.0%
GGGACGGTGGTTGTTGGGTT+TGG + chr7.1:32178519-32178538 None:intergenic 60.0%
TACGGACCAGACGGTGGAGA+CGG + chr7.1:32178622-32178641 None:intergenic 60.0%
! ACGGCGGAGCAACACTGGTT+GGG + chr7.1:32178579-32178598 None:intergenic 60.0%
! ATCCTCGGTGACGGCAACGT+CGG - chr7.1:32178699-32178718 MS.gene99506:CDS 60.0%
!! GAGACGTTCGGTGCTGACAG+CGG + chr7.1:32178763-32178782 None:intergenic 60.0%
ACGGACCAGACGGTGGAGAC+GGG + chr7.1:32178621-32178640 None:intergenic 65.0%
ACTGGTTGGGGGAGACTGCG+TGG + chr7.1:32178566-32178585 None:intergenic 65.0%
CGTCGGGGACGGTGGTTGTT+GGG + chr7.1:32178524-32178543 None:intergenic 65.0%
CTGGTTGGGGGAGACTGCGT+GGG + chr7.1:32178565-32178584 None:intergenic 65.0%
GACGGCGGAGCAACACTGGT+TGG + chr7.1:32178580-32178599 None:intergenic 65.0%
GACTGCGTGGGTGTGGTAGC+GGG + chr7.1:32178553-32178572 None:intergenic 65.0%
TGGCAACCAACGCTCCGGCA+CGG - chr7.1:32178899-32178918 MS.gene99506:CDS 65.0%
! ACGGACTACGGACCAGACGG+TGG + chr7.1:32178628-32178647 None:intergenic 65.0%
! CGGCGGAGCAACACTGGTTG+GGG + chr7.1:32178578-32178597 None:intergenic 65.0%
! GGATGACGGCGGAGCAACAC+TGG + chr7.1:32178584-32178603 None:intergenic 65.0%
! GGCGGAGCAACACTGGTTGG+GGG + chr7.1:32178577-32178596 None:intergenic 65.0%
!! ACGTTCGGTGCTGACAGCGG+CGG + chr7.1:32178760-32178779 None:intergenic 65.0%
!! GCGACGGACTACGGACCAGA+CGG + chr7.1:32178631-32178650 None:intergenic 65.0%
CAACCAACGCTCCGGCACGG+TGG - chr7.1:32178902-32178921 MS.gene99506:CDS 70.0%
CAACCACCGTGCCGGAGCGT+TGG + chr7.1:32178908-32178927 None:intergenic 70.0%
CCAGACGGTGGAGACGGGTG+CGG + chr7.1:32178616-32178635 None:intergenic 70.0%
CGGGGAGGCCGGAGATGACA+CGG + chr7.1:32178740-32178759 None:intergenic 70.0%
GCGTCGGGGACGGTGGTTGT+TGG + chr7.1:32178525-32178544 None:intergenic 70.0%
TGCCGACGTTGCCGTCACCG+AGG + chr7.1:32178704-32178723 None:intergenic 70.0%
TGGGGGAGACTGCGTGGGTG+TGG + chr7.1:32178560-32178579 None:intergenic 70.0%
!! CGTTCGGTGCTGACAGCGGC+GGG + chr7.1:32178759-32178778 None:intergenic 70.0%
!! GTGGGTGTGGTAGCGGGCGA+TGG + chr7.1:32178547-32178566 None:intergenic 70.0%
!! GTGGTAGCGGGCGATGGCGT+CGG + chr7.1:32178541-32178560 None:intergenic 70.0%
!! GTTCGGTGCTGACAGCGGCG+GGG + chr7.1:32178758-32178777 None:intergenic 70.0%
!! TGGTAGCGGGCGATGGCGTC+GGG + chr7.1:32178540-32178559 None:intergenic 70.0%
CCGCACCCGTCTCCACCGTC+TGG - chr7.1:32178613-32178632 MS.gene99506:CDS 75.0%
!! GGTAGCGGGCGATGGCGTCG+GGG + chr7.1:32178539-32178558 None:intergenic 75.0%
! GCTGACAGCGGCGGGGAGGC+CGG + chr7.1:32178751-32178770 None:intergenic 80.0%
!! GCGGGCGATGGCGTCGGGGA+CGG + chr7.1:32178535-32178554 None:intergenic 80.0%
!! GGCGATGGCGTCGGGGACGG+TGG + chr7.1:32178532-32178551 None:intergenic 80.0%
!!! CGGTGCTGACAGCGGCGGGG+AGG + chr7.1:32178755-32178774 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 32178510 32179070 32178510 ID=MS.gene99506
chr7.1 mRNA 32178510 32179070 32178510 ID=MS.gene99506.t1;Parent=MS.gene99506
chr7.1 exon 32178510 32179070 32178510 ID=MS.gene99506.t1.exon1;Parent=MS.gene99506.t1
chr7.1 CDS 32178510 32179070 32178510 ID=cds.MS.gene99506.t1;Parent=MS.gene99506.t1
Gene Sequence

>MS.gene99506

ATGTTACCAAACCCAACAACCACCGTCCCCGACGCCATCGCCCGCTACCACACCCACGCAGTCTCCCCCAACCAGTGTTGCTCCGCCGTCATCCAACAAATCGCCGCACCCGTCTCCACCGTCTGGTCCGTAGTCCGTCGCTTCGACAATCCACAAGCCTACAAACACTTCGTCAAAAGCTGCCATGTAATCCTCGGTGACGGCAACGTCGGCACTCTCCGTGAAGTCCGTGTCATCTCCGGCCTCCCCGCCGCTGTCAGCACCGAACGTCTCGAAGTCCTAGACGATGAACGTCATGTCATCAGCTTCAGCATGATCGGTGGTGATCACCGGCTTGCTAACTACCGTTCTGTCACCACTCTTCACCCTAGTCCGATCTCCGACGAAGATGGCAACCAACGCTCCGGCACGGTGGTTGTTGAATCCTACGTTGTTGATGTTCCACCTGGAAACACCACTGAAGATACATGTGTGTTTGTTGATACTATTCTTCGGTGCAACCTTCAATCTCTCGCGAAATTTGCTGAGAATTTGGCCTCAACGAGATCAAATCAACGATAA

Protein sequence

>MS.gene99506.t1

MLPNPTTTVPDAIARYHTHAVSPNQCCSAVIQQIAAPVSTVWSVVRRFDNPQAYKHFVKSCHVILGDGNVGTLREVRVISGLPAAVSTERLEVLDDERHVISFSMIGGDHRLANYRSVTTLHPSPISDEDGNQRSGTVVVESYVVDVPPGNTTEDTCVFVDTILRCNLQSLAKFAENLASTRSNQR